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Aorta

The aorta is the largest blood vessel in the human body, originating from the left ventricle of the heart and extending down through the chest and abdomen.
It plays a crucial role in distributing oxygenated blood throughout the body.
Researchers studying the aorta and related cardiovascular conditions can leverage PubCompare.ai's AI-driven platform to optimize their research process.
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Most cited protocols related to «Aorta»

Trial documentation including protocols, SOPs and CRFs will be shared electronically with participating study centres. Protocol amendments will be submitted to the Royal Brisbane and Women’s Hospital HREC and local site research governance offices and disseminated to the relevant parties at each study site. Data recorded on printed CRFs will be scanned to the study centre in Townsville where it will be entered centrally and examined for data quality. This will allow confirmation of entry criteria and collection of set entry and outcome data. Examples of important baseline data which will be collected include age, gender, presence of diabetes and/or dyslipidaemia, concurrent medications and maximum aortic diameter. At completion of the trial the database will be checked for errors and data confirmed with source documentation where required. Analysis of primary and secondary endpoints will be based on intention-to-treat at the time of randomisation. All participants who meet the eligibility criteria, provide written informed consent and are enrolled in the study will be included in the primary analysis, regardless of adherence to medication allocation.
To identify potential confounders, collected clinical and demographic data will be compared between groups via univariate statistics. The distribution of all continuous data variables will be assessed for normality using the Kolgorov-Smirnov test. Normally distributed continuous variables will be compared between test groups via t test; non-normally continuous distributed variables will be compared between groups using the Mann-Whitney U test. Nominal data will be compared using the chi-squared test. Characteristics showing a p value < 0.100 on univariate tests will be considered as potential confounders and will be entered as covariates in subsequent binary logistic regression models assessing the association of each of the outcome measures with treatment allocation. Following binary logistic regression, the association of all covariates with treatment allocation will be reported as odds ratios and 95% confidence intervals. For all analyses, p values <0.05 will be considered to be significant. Data will be published in a peer-reviewed journal with copies of the paper available to participants if required.
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Publication 2017
Aorta Diabetes Mellitus Dyslipidemias Eligibility Determination Gender Pharmaceutical Preparations Woman
The Iso-Seq method for sequencing full-length transcripts was developed by PacBio during the same time period as the genome assembly. We therefore used this technique to improve characterization of transcript isoforms expressed in cattle tissues using a diverse set of tissues collected from L1 Dominette 0 1449 upon euthanasia. The data were collected using an early version of the Iso-Seq library protocol [26 ] as suggested by PacBio. Briefly, RNA was extracted from each tissue using Trizol reagent as directed (Thermo Fisher). Then 2 μg of RNA were selected for PolyA tails and converted into complementary DNA (cDNA) using the SMARTer PCR cDNA Synthesis Kit (Clontech). The cDNA was amplified in bulk with 12–14 rounds of PCR in 8 separate reactions, then pooled and size-selected into 1–2, 2–3, and 3–6 kb fractions using the BluePippin instrument (Sage Science). Each size fraction was separately re-amplified in 8 additional reactions of 11 PCR cycles. The products for each size fraction amplification were pooled and purified using AMPure PB beads (Pacific Biosciences) as directed, and converted to SMRTbell libraries using the Template Prep Kit v1.0 (PacBio) as directed. Iso-Seq was conducted for 22 tissues including abomasum, aorta, atrium, cerebral cortex, duodenum, hypothalamus, jejunum, liver, longissimus dorsi muscle, lung, lymph node, mammary gland, medulla oblongata, omasum, reticulum, rumen, subcutaneous fat, temporal cortex, thalamus, uterine myometrium, and ventricle from the reference cow, as well as the testis of her sire. The size fractions were sequenced in either 4 (for the smaller 2 fractions) or 5 (for the largest fraction) SMRTcells on the RS II instrument. Isoforms were identified using the Cupcake ToFU pipeline [27 ] without using a reference genome.
Short-read–based RNA-seq data derived from tissues of Dominette were available in the GenBank database because her tissues have been a freely distributed resource for the research community. To complement and extend these data and to ensure that the tissues used for Iso-Seq were also represented by RNA-seq data for quantitative analysis and confirmation of isoforms observed in Iso-Seq, we generated additional data, avoiding overlap with existing public data. Specifically, the TruSeq stranded mRNA LT kit (Illumina, Inc.) was used as directed to create RNA-seq libraries, which were sequenced to ≥30 million reads for each tissue sample. The Dominette tissues that were sequenced in this study include abomasum, anterior pituitary, aorta, atrium, bone marrow, cerebellum, duodenum, frontal cortex, hypothalamus, KPH fat (internal organ fat taken from the covering on the kidney capsule), lung, lymph node, mammary gland (lactating), medulla oblongata, nasal mucosa, omasum, reticulum, rumen, subcutaneous fat, temporal cortex, thalamus, uterine myometrium, and ventricle. RNA-seq libraries were also sequenced from the testis of her sire. All public datasets, and the newly sequenced RNA-seq and Iso-Seq datasets, were used to annotate the assembly, to improve the representation of low-abundance and tissue-specific transcripts, and to properly annotate potential tissue-specific isoforms of each gene.
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Publication 2020
Abomasum Anabolism Aorta Bone Marrow Capsule Cattle cDNA Library Cerebellum Cerebral Ventricles Cortex, Cerebral Dietary Fiber DNA, Complementary Duodenum Euthanasia Genes Genome Heart Atrium Hypothalamus Jejunum Kidney Liver Lobe, Frontal Lung Mammary Gland Medulla Oblongata Muscle Tissue Myometrium Nasal Mucosa Nodes, Lymph Omasum Pituitary Hormones, Anterior Poly(A) Tail Protein Isoforms Reticulum RNA, Messenger RNA-Seq Rumen Subcutaneous Fat Temporal Lobe Testis Thalamus Tissues Tissue Specificity Tofu trizol Uterus
Several BP measurement methods are now available. The main methods include catheterization, auscultation, oscillometry, volume clamping, and tonometry.
Catheterization is the gold standard method [6 (link)]. This method measures instantaneous BP by placing a strain gauge in fluid contact with blood at any arterial site (e.g., radial artery, aorta). However, the method is invasive.
Auscultation, oscillometry, and volume clamping are noninvasive methods. These methods employ an inflatable cuff.
Auscultation is the standard clinical method [7 (link)]. This method measures systolic and diastolic BP by occluding an artery with a cuff and detecting the Korotkoff sounds using a stethoscope and manometer during cuff deflation. The first sound indicates the initiation of turbulent flow and thus systolic BP, while the fifth sound is silent and indicates the renewal of laminar flow and thus diastolic BP.
Oscillometry is the most popular non-invasive, automatic method [8 (link), 9 (link)]. This method measures mean, diastolic, and systolic BP by also using a cuff but with a pressure sensor inside it. The measured cuff pressure not only rises and falls with cuff inflation and deflation but also shows tiny oscillations indicating the pulsatile blood volume in the artery. The amplitude of these oscillations varies with the applied cuff pressure, as the arterial elasticity is nonlinear. The BP values are estimated from the varying oscillation amplitudes using the empirical fixed-ratios principle. When evaluated against auscultation using an Association for the Advancement of Medical Instrumentation (AAMI) protocol, some oscillometric devices achieve BP errors within the AAMI limits of 5 mmHg bias and 8 mmHg precision [10 ]. However, oscillometry is unreliable in subjects with certain conditions such as atrial fibrillation, stiff arteries, and pre-eclampsia [11 ].
Volume clamping is a non-invasive, automatic method used in research [12 (link), 13 ]. This method measures instantaneous (finger) BP by using a cuff and a photoplethysmography (PPG) sensor to measure the blood volume (see Section V.A). The blood volume at zero transmural pressure is estimated via oscillometry. The cuff pressure is then continually varied to maintain this blood volume throughout the cardiac cycle via a fast servo-control system. The applied cuff pressure may thus equal BP. Volume clamping devices also achieve BP errors within AAMI limits when evaluated against auscultation and near AAMI limits when evaluated against radial artery catheterization [14 (link)].
However, cuff use has several drawbacks. In particular, cuffs are cumbersome and time consuming to use, disruptive during ambulatory monitoring, especially while sleeping, and do not readily extend to low resources settings.
Tonometry is another non-invasive method used in research that, in theory, does not require an inflatable cuff [15 , 16 ]. This method measures instantaneous BP by pressing a manometer-tipped probe on an artery. The probe must flatten or applanate the artery so that its wall tension is perpendicular to the probe. However, manual and automatic applanation have proven difficult. As a result, in practice, the measured waveform has been routinely calibrated with cuff BP whenever a BP change is anticipated [17 (link)].
In sum, the existing BP measurement methods are invasive, manual, or require a cuff. So, none are suitable for ubiquitous (i.e., ultra-convenient, unobtrusive, and low cost) monitoring.
Publication 2015
Aorta Arteries Arteries, Radial Atrial Fibrillation Auscultation BLOOD Blood Pressure Blood Volume Cardiac Volume Catheterization Clinical Protocols Diastole Elasticity Fingers Gold Manometry Medical Devices Oscillometry Photoplethysmography Pre-Eclampsia Pressure Pressure, Diastolic Sound Stethoscopes Strains Systole Systolic Pressure Tonometry
The GBD study attributes each death to a single underlying cause that began the series of events leading to death, in accordance with ICD principles. The GBD study organises causes of death in a hierarchical list containing four levels (appendix 1 section 7). At the highest level (Level 1), all disease burden is divided among three mutually exclusive and collectively exhaustive categories: communicable, maternal, neonatal, and nutritional (CMNN) diseases; non-communicable diseases (NCDs); and injuries. Level 2 distinguishes these Level 1 categories into 21 cause groups, such as cardiovascular diseases; diarrhoeal diseases, lower respiratory infections (LRIs), and other common infectious diseases; or transport injuries. Level 3 disaggregates these causes further; in most cases this disaggregation represents the finest level of detail by cause, such as stroke, ischaemic heart disease, or road injuries. Where data are sufficiently available or specific policy relevance has been sought, selected causes are further disaggregated at Level 4, such as drug-susceptible tuberculosis, multidrug-resistant tuberculosis without extensive drug resistance, and extensively drug-resistant tuberculosis. For GBD 2017, the cause hierarchy was further refined to separately estimate causes with substantial policy interest or high levels of burden. Specific changes included separate estimation of non-rheumatic calcific aortic and degenerative mitral valve diseases, and myelodysplastic, myeloproliferative, and other haemopoietic neoplasms, resulting in a reduction in the estimates of some residual causes. Disaggregation of residual causes also allowed separate estimation of type 1 and type 2 diabetes, chronic kidney disease due to type 1 and type 2 diabetes, poisoning by carbon monoxide, liver cancer due to non-alcoholic steatohepatitis (NASH), subarachnoid haemorrhage, ectopic pregnancy, and invasive non-typhoidal salmonella. Maternal and neonatal disorders, previously estimated as separate cause groupings at Level 2 of the hierarchy, were estimated for GBD 2017 at Level 3 of the hierarchy, and then aggregated up to Level 2 to better capture the epidemiological connections and linked burden between them. The complete hierarchy of causes included in GBD 2017 and their corresponding ICD9 and ICD10 codes are described in appendix 1 (section 7).
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Publication 2018
Aorta Cancer of Liver Carbon Monoxide Poisoning Cardiovascular Diseases Cerebrovascular Accident Chronic Kidney Diseases Communicable Diseases Diabetes Mellitus, Non-Insulin-Dependent Diarrhea Ectopic Pregnancy Extensively Drug-Resistant Tuberculosis Hematopoietic Neoplasms Infant, Newborn Injuries Mitral Valve Mothers Myocardial Ischemia Neonatal Diseases Nonalcoholic Steatohepatitis Noncommunicable Diseases Nutrition Disorders Pharmaceutical Preparations Resistance, Drug Respiratory Tract Infections Salmonella Subarachnoid Hemorrhage Tuberculosis Tuberculosis, Multidrug-Resistant Typhoid Fever
C57BL/6, ApoE−/−or ApoE−/−(WD) mice were anesthetized and their vasculature was perfused by cardiac puncture with PBS containing 20 U/ml of heparin to remove blood from all vessels. Under a dissection microscope, we carefully harvested whole aortas by pulling off all of the adipose tissue and collecting all aortic layers including the adventitia. To fully characterize the collected aortas, we measured aortic wall thickness and adventitial thickness in paraffin-cut sections. In addition, the dissected aortas were weighed to control the total amount of collected aortic tissues. Harvested aortas were microdissected and digested with 125 U/ml collagenase type XI, 60 U/ml hyaluronidase type I-s, 60 U/ml DNase1, and 450 U/ml collagenase type I (all enzymes were obtained from Sigma-Aldrich) in PBS containing 20 mM Hepes at 37°C for 1 h. A cell suspension was obtained by mashing the aorta through a 70-μm strainer. Cells were incubated with Abs for 20 min at 4°C, washed twice, and incubated with secondary Abs for an additional 20 min. After washing, immunofluorescence was detected by flow cytometry (FACSCalibur or CyanADP), data were analyzed using WinMDI (The Scripps Research Institute) or FlowJO (Tree Star Inc.) software. Abs used were as follows: allophycocyanin (APC)-Cy7 or PE-Texas red-CD45, FITC, PE or PerCP-CD3, FITC, APC-TCRβ, PE-Cy5 or APC-CD19, PE-B220, PE-I-Ab, PerCP-Cy5.5-Mac-1, PE or APC-CD11c (all Abs were obtained from BD Biosciences) and FITC-CD68 (Serotec). In some experiments, the aortas from two to three mice were pooled and analyzed. In some experiments, LN or spleens from C57BL/6 mice were collected and split in two parts: one part of pLN (or spleen) was treated with the enzyme cocktail (see previous paragraph) and the other was kept at 4°C. After 1 h, the expression of CD45, TCR, CD19, I-Ab antigens was determined by flow cytometry.
Publication 2006
Adventitia allophycocyanin Antigen T Cell Receptor, beta Chain Aorta ApoE protein, human Blood Vessel Cells Collagenase Collagenase, Clostridium histolyticum CY5.5 cyanine dye Dissection Enzymes Flow Cytometry Fluorescein-5-isothiocyanate Heart Heparin HEPES Hyaluronidase I-antigen Immunofluorescence Macrophage-1 Antigen Mice, Inbred C57BL Microscopy Mus Paraffin Punctures Spleen Tissue, Adipose Trees

Most recents protocols related to «Aorta»

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Example 5

Methods.

Experiments were performed in male apoE−/− mice fed an atherogenic diet (D12108, cholate-free AIN-76A semi-purified diet, Research Diets Inc., New Brunswick, NJ) from 4 weeks of age. MPE-298 (300 nmol/kg), MPE-267 (300 nmol/kg) or vehicle (0.9% NaCl), were administered by daily by subcutaneous (s.c.) injections for 8 weeks, from 12 weeks of age, as shown schematically in FIG. 8A.

Results and Discussion.

The results depicted in FIG. 8B show that chronic treatment of the animals with cyclic peptides MPE-267 and MPE-298 reduced aortic lesions by 28% and 42% (p<0.01), respectively, relative to 0.9% NaCl (vehicle), providing evidence that the cyclic peptides exhibit anti-atherosclerotic activity.

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Patent 2024
Animals Aorta Apolipoproteins E Atherosclerosis Cholate Cyclic Peptides Diet Males Mus Normal Saline
After decapitation, an emergency thoracotomy was performed, and rat hearts were isolated, attached via an aortic cannula, and retrogradely perfused using the Langendorff technique at a gradually increasing perfusion pressure between 40 – 120 cm H2O [12 (link)]. The hearts were perfused with Krebs–Henseleit solution (118 mM NaCl, 4.7 mM KCl, 2.5 mM CaCl2 2H2O, 1.7 mM MgSO4 H2O, 25 mM NaHCO3, 1.2 mM KH2PO4, 5.5 mM glucose, equilibrated with 95% O2/5% CO2) and warmed to 37 °C (pH = 7.4). After heart perfusion commenced, a 30-min period was allowed for the hearts to stabilize. A transducer (BS473-0184, Experimetria Ltd., Budapest, Hungary) was used to monitor the following parameters of myocardial function: maximum rate of left ventricular pressure development (dp/dt max), minimum rate of left ventricular pressure development (dp/dt min), systolic left ventricular pressure (SLVP), diastolic left ventricular pressure (DLVP), heart rate (HR). The coronary flow (CF) was measured flowmetrically.
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Publication 2023
Aorta Bicarbonate, Sodium Cannula Decapitation Emergencies Glucose Heart Krebs-Henseleit solution Left Ventricles Myocardium Perfusion Pressure Pressure, Diastolic Rate, Heart Sodium Chloride Sulfate, Magnesium Systolic Pressure Thoracotomy Transducers
In accordance with the curriculum of the Board Certification of Surgery of JSS, the fields of CST and R&D were classified into the following eight categories: 1. digestive and abdominal organs; 2. mammary glands; 3. thoracic; 4. heart, aorta, and vena cava; 5. peripheral blood vessels; 6. head and neck, body surface, and endocrine; 7. pediatric; and 8. trauma. The subclassification of each field was done by organ.
Publication 2023
Abdomen Aorta Blood Vessel Digestive System Head Heart Mammary Gland Operative Surgical Procedures System, Endocrine Tympanicum, Glomus Venae Cavae Wounds and Injuries
To assess the diagnostic accuracy of the rAAA I71.3 code, a detailed retrospective review of patients was undertaken from the following centers located in various regions of Seoul: Seoul National University Hospital, Seoul Metropolitan Government-Seoul National University Boramae Medical Center, Korea University Guro Hospital, and Korea Kyunghee Medical Center. All ICD-10 code I71.3 diagnosed patients from the period of January 1, 2000 to December 31, 2020 at each center were identified. The CT images and surgical records of these patients were reviewed by at least 2 physicians at each center.
True rAAA was defined as degenerative AAA with anterior intraperitoneal rupture, posterior retroperitoneal rupture, or aortic fistula to surrounding organs including inferior vena cava, duodenum, or renal vessels [11 ]. The code cases of I71.3 that did not fit the above definition were categorized as misdiagnoses. This included patients with nondegenerative AAA ruptures due to infection or aortitis, thoracic aorta aneurysms, non-ruptures such as impending ruptures, and patients with uncertain data. Uncertain data includes patients with missing CT images and discordant surgical records that state intact AAA without rupture, despite diagnosis with I71.3 code.
Publication 2023
Aorta Aortic Aneurysm, Thoracic Aortitis Blood Vessel Diagnosis Duodenum Fistula Infection Kidney MLL protein, human Operative Surgical Procedures Patients Physicians Retroperitoneal Space Vena Cavas, Inferior
The procedure code system in Korea is based on the Korean Health Insurance Classification of Procedures in Medicine. Unlike the ICD-10, it is complex and there are multiple treatment codes that are applicable to the treatment of AAA. The open repair codes for AAA are O0023, Resection of Aneurysm-Abdominal Aorta (suprarenal and juxtarenal); O0224, Resection of Aneurysm-Abdominal Aorta (infrarenal); and O02034, Resection of Aneurysm-Abdominal Aorta and Iliac Artery. The endovascular aneurysm repair (EVAR) codes for AAA are M6603, Percutaneous Intravascular Installation of Metallic Stent-Aortic; M6611, Percutaneous Intravascular Installation of Stent Graft-Aortic; and M6612, Percutaneous Intravascular Installation of Stent Graft-Aortic and Iliac.
An additional operational definition combining both I71.3 code and treatment codes for both EVAR and open repair were added to extract rAAA patients at each hospital was performed. The addition of this operational definition was attempted to increase diagnostic specificity. The CT images and surgical records of these patients were reviewed to identify true rAAA according to the same definitions as above.
Publication 2023
Aorta Aortic Aneurysm, Abdominal Diagnosis Endovascular Aneurysm Repair Grafts Health Insurance Iliac Artery Ilium Inpatient Koreans Metals Operative Surgical Procedures Pharmaceutical Preparations Stents

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More about "Aorta"

The aorta is the largest and most important artery in the human body, playing a crucial role in distributing oxygenated blood throughout the system.
Originating from the left ventricle of the heart, this vital blood vessel extends down through the chest and abdomen, carrying the lifeblood to tissues and organs.
Researchers studying the aorta and related cardiovascular conditions can leverage PubCompare.ai's innovative AI-driven platform to optimize their research process.
PubCompare.ai's intuitive tools help locate relevant protocols from literature, preprints, and patents, while utilizing advanced AI-driven comparisons to identify the best protocols and products.
This seamless research experience allows researchers to save valuable time on protocol selection and validation, enhancing the reproducibility and accuracy of their studies.
Aortic research often involves the use of various scientific techniques and materials, such as FBS (Fetal Bovine Serum) for cell culture, TRIzol reagent for RNA extraction, DMEM (Dulbecco's Modified Eagle Medium) as a cell culture medium, Oil Red O for lipid staining, RNeasy Mini Kit for RNA purification, Penicillin/streptomycin for antibiotics, Vevo 2100 for ultrasound imaging, Image-Pro Plus 6.0 for image analysis, and Collagenase type II for tissue digestion.
PubCompare.ai's platform can assist researchers in navigating these tools and techniques, ensuring efficient and effective aortic research.
By leveraging PubCompare.ai's AI-driven platform, researchers can streamline their aortic studies, saving time and enhancing the quality of their work.
This innovative solution is a game-changer for those seeking to optimize their cardiovascular research process.