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Bronchus, Primary

The primary bronchus, also known as the principal bronchus, is the main airway that branches off from the trachea and leads to the lungs.
It divides into the left and right primary bronchi, which then branch further into smaller secondary and tertiary bronchi.
The primary bronchi play a crucial role in the respiratory system, facilitating the passage of air into and out of the lungs.
Understanding the anatomy and function of the primary bronchus is essential for researchers and scientists studying respiratory health, disease, and interventions.
PubCompare.ai's innovative AI-driven comparisons can help optimize research protocols for reproducibility and accuracy, allowing you to discover the best protocols and products by comparing data from literature, preprints, and patents.

Most cited protocols related to «Bronchus, Primary»

1
Quantifying Lung Radiograph Opacities
Details of the RALE score are provided in Figure 2. To determine the RALE score, each radiograph was divided into quadrants, defined vertically by the vertebral column and horizontally by the first branch of the left main bronchus. Each quadrant was assigned a consolidation score from 0–4 to quantify the extent of alveolar opacities, based on the percentage of the quadrant with opacification and a density score from 1–3 to quantify the overall density of alveolar opacities, unless the consolidation score for that quadrant was 0. The density score (1=hazy, 2=moderate, 3=dense) allows for more quantitative assessment of the density of opacification by quadrant. To calculate the final RALE score, the product of the consolidation and density score for each quadrant were summed for a final RALE score ranging from 0 (no infiltrates) to 48 (dense consolidation in >75% of each quadrant).
In the organ donors, chest radiographs obtained within 24 hours of lung procurement were scored for each donor, using the radiograph obtained closest to the time of procurement. Radiographs were scored by consensus of two physician reviewers without knowledge of clinical data or lung weight. In FACTT, each radiograph was scored independently by two physician reviewers blinded to treatment arm, in order to examine inter-observer reliability.
Warren M.A., Zhao Z., Koyama T., Bastarache J.A., Shaver C.M., Semler M.W., Rice T.W., Matthay M.A., Calfee C.S, & Ware L.B. (2018). Severity Scoring of Lung Edema on the Chest Radiograph Is Associated with Clinical Outcomes in ARDS. Thorax, 73(9), 840-846.
Publication 2018
Bronchus, Primary Donor, Organ Donors Lung Physicians Radiography Radiography, Thoracic Vertebral Column
2
CFTR Function Assays in Cell Lines
The CF bronchial epithelial cells CFBE41o-, with stable expression of F508del-CFTR or wild type CFTR, were obtained by Dr. J.P. Clancy (2 (link)). A549 and FRT cells were stable transfected with a plasmid coding for F508del-CFTR and carrying the resistance gene for zeocin. The three cell types were also transfected with the halide-sensitive yellow fluorescent protein (HS-YFP) YFP-H148Q/I152L (8 (link)). The culture media were: Coon’s modified Ham’s F-12 medium for FRT cells, DMEM/Ham’s F12 (1:1) for A549 cells, and MEM for CFBE41o-. All media were supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin. For fluorescence assays of CFTR activity, FRT, A549 and CFBE41o- cells were plated (50,000 cells/well) on clear-bottom 96-well black microplates (Corning Life Sciences, Acton, MA). For short-circuit current experiments, 500,000 CFBE41o- cells were seeded into Snapwell permeable supports (Corning Life Sciences).
Primary human bronchial epithelial cells from F508del/F508del CF patients were cultured as previously described (9 (link), 28 (link)). Briefly, cells were initially cultured on plastic in proliferative serum-free medium, LHC9/RPMI 1640, and then cultured on Snapwell supports for 10-12 days with a differentiating medium.
Sondo E., Tomati V., Caci E., Esposito A.I., Pfeffer U., Pedemonte N, & Galietta L.J. (2011). RESCUE OF THE MUTANT CFTR CHLORIDE CHANNEL BY PHARMACOLOGICAL CORRECTORS AND LOW TEMPERATURE ANALYZED BY GENE EXPRESSION PROFILING. American journal of physiology. Cell physiology, 301(4), C872-C885.
Publication 2011
A549 Cells Biological Assay Bronchi Bronchus, Primary Cells CFTR protein, human Epithelial Cells Fetal Bovine Serum Fluorescence Genes Glutamine Homo sapiens Patients Penicillins Permeability Plasmids Proteins Serum Streptomycin Zeocin
3
Rigorous Mediastinal Lymph Node Dissection

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Publication 2011
Aortopulmonary Septal Defect Arm, Upper Bronchi Bronchus, Primary Cryoultramicrotomy Dissection Eligibility Determination Esophagus Ethics Committees, Research Ligaments Lung Lung Neoplasms Lymph Node Dissection Lymph Node Excision Lymphoid Tissue Malignant Neoplasms Mediastinal Neoplasms Mediastinoscopy Mediastinum Mucocutaneous Lymph Node Syndrome Muscle Rigidity Neoplasms Nervousness Nodes, Lymph Non-Small Cell Lung Carcinoma Patients Pericardium Stem, Plant Surgeons Teaching Thoracic Surgery, Video-Assisted Thoracotomy Trachea Trunks, Brachiocephalic Vena Cavas, Superior
4
Isolation of Lung-Associated Lymph Nodes
Animals were killed and the pulmonary and systemic circulation was perfused with saline/EDTA to remove the intravascular pool of cells. Paratracheal and parathymic intrathoracic LNs were collected. Lungs were carefully separated from thymic and cardiovascular remnants and removed in toto, including the main bronchi and trachea. Due to the photosensitivity of the FITC material, organs from FITC-macromolecule–instilled animals were protected from direct light throughout the manipulation. Organs were thoroughly minced using iridectomy scissors and incubated for 30 min in digestion medium in a humidified incubator at 37°C and 5% CO2, according to a modified protocol 21. Organ fragments were resuspended, fresh digestion medium was added, and incubation was extended for another 15 min. After a final resuspension, very few organ debris were left. Samples were centrifuged and resuspended in calcium and magnesium–free PBS containing 10 mM EDTA for 5 min at room temperature on a shaker. Finally, the cells were subjected to RBC lysis, washed in FACS-EDTA, passed through a 50-μm cell strainer, and kept on ice until labeling. Cell viability after this procedure was consistently >95%.
Vermaelen K.Y., Carro-Muino I., Lambrecht B.N, & Pauwels R.A. (2001). Specific Migratory Dendritic Cells Rapidly Transport Antigen from the Airways to the Thoracic Lymph Nodes. The Journal of Experimental Medicine, 193(1), 51-60.
Publication 2001
1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-thiazole)-2-methylidene)quinolinium Animal Organs Animals Bronchus, Primary Calcium Cardiovascular System Cells Cell Survival Digestion Edetic Acid Fluorescein-5-isothiocyanate Iridectomy Light Lung Magnesium Pepsin A Photosensitization Saline Solution Thymus Gland Trachea
5
Harvesting Mucus from Human Bronchial Epithelial Cells
Mucus was harvested from primary human bronchial epithelial (HBE) cell cultures as previously described [18] (link), [19] , [21] . Briefly, excess surgical tissue was procured by the UNC Tissue Core Facility. Normal human bronchial epithelial cells were cultured on a 0.4 mm pore-sized Millicell (Millipore, Billerica, MA) coated with collagen and maintained in air-liquid interface media (UNC Tissue Core) as described in [29] (link). Over a period of 6 weeks, confluent cultures developed cilia, generated and established a periciliary liquid (PCL) layer surrounding the cilia, a mucus layer, and the HBE culture transported mucus. Washings from >100 cultures were pooled and then concentrated against Spectra/Gel to the desired wt%. Concentrated mucus was dialyzed against PBS to insure isotonicity as previously described [18] (link), [21] .
Hill D.B., Vasquez P.A., Mellnik J., McKinley S.A., Vose A., Mu F., Henderson A.G., Donaldson S.H., Alexis N.E., Boucher R.C, & Forest M.G. (2014). A Biophysical Basis for Mucus Solids Concentration as a Candidate Biomarker for Airways Disease. PLoS ONE, 9(2), e87681.
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Publication 2014
Bronchi Bronchus, Primary Cilia Collagen Epithelial Cells Homo sapiens Mucus Operative Surgical Procedures Tissues

Most recents protocols related to «Bronchus, Primary»

1
SCLC Patients in SEER Database
Because the PI status has been recorded since 2010 based on the term, cs site-specific factor 2, patients were identified from the SEER database between 2010 and 2018. The inclusion criteria were as follows: (a) malignant tumor located in the main bronchus and lung (Site code: C340-C349); (b) patients diagnosed with primary SCLC (Histology code: 8002, 8041, 8042, 8043, 8044, 8045); (c) diagnostic confirmation based on positive histology or positive microscopic confirmation; (d) the status of PI was recorded clearly; (e) T stage, N stage, and M stage according to the 7th edition AJCC staging system was complete. Besides, clinical variables including age at diagnosis, sex, race, grade, tumor site, laterality, surgery, radiotherapy, and chemotherapy were contained. The exclusion criteria were as follows: (a) survived less than 1 month after diagnosis; (b) patients aged < 18 years; (c) unknown data on race, marital, laterality, surgery, radiotherapy, and chemotherapy. Finally, a total of 1,770 patients met the criteria in the original cohort and 449 patients in the prognostic cohort. Age at diagnosis was divided into under 60 years old, 60–70 years old, and over 70 years old. Tumor grade was grouped into grade I–II, grade III, grade IV, and unknown. All the selected processes of the two study cohorts were exhibited in Figure 1.
Yang J., Yin H., Liu M., Zou G, & Yu B. (2023). Effect of pleural invasion on survival of patients with small cell lung cancer: Propensity score analysis and nomogram establishment based on the SEER database. Frontiers in Surgery, 10, 1108732.
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Publication 2023
Bronchus, Primary Diagnosis Division Phase, Cell Lung Malignant Neoplasms Microscopy Neoplasms Operative Surgical Procedures Patients Pharmacotherapy Radiotherapy Small Cell Lung Carcinoma
2
Differentiation of Primary Human Bronchial Epithelial Cells
Primary human bronchial epithelial cells (HBE) were provided by Nationwide Children’s Hospital Cure Cystic Fibrosis Columbus Epithelial Cell Core (Columbus, OH) without identifiers (exempt status from the Institutional Review Board). Primary HBE cells were cultured as previously reported in our lab [38 (link),70 (link)]. Briefly, primary human airway cells were seeded onto 6.5mm Transwell filters (Corning) coated with collagen Type IV (Sigma) with ~50,000 cells. Cells were seeded and fed with ROCK inhibitor-supplemented air-liquid interface (ALI) media on both sides of the membrane until optimal epithelial integrity was reached, as measured by an epithelial voltohmeter (~7 days; ~300 Ohms·cm2). Basal medium was replaced with PneumaCult-ALI (Stem Cell Technologies) differentiation medium, and cells were fed three times per week for ~3 weeks until fully differentiated. Cell differentiation and integrity of the bronchial epithelium were confirmed before each experiment. Cultures were also checked for absence of mycoplasma contamination.
Fullen A.R., Gutierrez-Ferman J.L., Rayner R.E., Kim S.H., Chen P., Dubey P., Wozniak D.J., Peeples M.E., Cormet-Boyaka E, & Deora R. (2023). Architecture and matrix assembly determinants of Bordetella pertussis biofilms on primary human airway epithelium. PLOS Pathogens, 19(2), e1011193.
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Publication 2023
Bronchi Bronchus, Primary Cells Collagen Type IV Cystic Fibrosis Differentiations, Cell Epithelial Cells Epithelium Ethics Committees, Research Homo sapiens Mycoplasma Stem Cells Tissue, Membrane
3
Isolation and Culture of Primary Bronchial Epithelial Cells
Primary bronchial epithelial cells (PBECs) were obtained from bronchial brushings in six healthy subjects (Additional file 1: Table S1). The Medical Ethics Committee of Huashan Hospital approved the study (KY2019-508), and all subjects gave their written informed consent. After protease digestion and centrifugation (1200 rpm, 5 min), PBECs were cultured in bronchial epithelial cell medium (#3211, ScienCell, San Diego, California, USA), supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin and 1 × bronchial epithelial cell growth supplement (#3262, ScienCell, San Diego, California, USA) using poly-L-lysine-coated flasks and employed for experiments at passage 2–4 without mycoplasma contamination (Additional file 2: Fig. S1). Human bronchial epithelial cells (HBECs, ZQ0001, ScienCell, San Diego, California, USA) were cultured in Keratinocyte Medium (#2111, ScienCell, San Diego, California, USA), supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin and 1 × keratinocyte growth supplement (#2162, ScienCell, San Diego, California, USA). Unless otherwise stated, cell culture reagents were purchased from Gibco (Carlsbad, CA, USA).
Song Y., Fu W., Zhang Y., Huang D., Wu J., Tong S., Zhong M., Cao H, & Wang B. (2023). Azithromycin ameliorated cigarette smoke-induced airway epithelial barrier dysfunction by activating Nrf2/GCL/GSH signaling pathway. Respiratory Research, 24, 69.
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Publication 2023
Bronchi Bronchus, Primary Cell Culture Techniques Centrifugation Culture Media Dietary Supplements Digestion Epithelial Cells Ethics Committees, Clinical Healthy Volunteers Homo sapiens Keratinocyte Lysine Mycoplasma Penicillins Peptide Hydrolases Poly A Streptomycin Toothbrushing
4
Quantification of Neuroendocrine Cells in Developing Lung
Neuroendocrine precursor cells (ASCL1 +) were counted on E14.5, 5 µm thick sections, in a square of 400 µm2 around the first branch of the main bronchi. Of each genotype and each n, 3 sections were counted and the percentage of NE cells were calculated based on the total number of DAPI + nuclei in the airway.
Neuroendocrine cells (ASCL1 + or ASCL1 + /CGRP +) were counted on E18.5, 5 µm thick sections. Of each genotype and each n, 1 or 2 sections of the left lobe were counted and the number of NE cells were normalized to airway (SOX2 +) area present in the section.
Fluorescent intensity of SOX2 and SOX21 was measured in three different location which contained ASCL1 + NEBs, in proximal SOX2 + SOX21 + airway epithelium. The mean fluorescence intensity (MFI) was calculated by dividing the fluorescence intensity of ASCL1 + or ASLC1- region compared to the fluorescence intensity of the total airway epithelium at that specific location.
Fluorescent intensity of TRP63, SOX2 and SOX21 was measured in a 150 µm2 box in the main bronchi, in 3 sections of 3 different mouse lungs. The MFI was calculated by dividing the fluorescence intensity of TRP63 + or TRP63- region compared to the fluorescence intensity of the total airway epithelium at that specific location.
Image J was used to analyze the pictures.
Eenjes E., Benthem F., Boerema-de Munck A., Buscop-van Kempen M., Tibboel D, & Rottier R.J. (2023). Distinct roles for SOX2 and SOX21 in differentiation, distribution and maturation of pulmonary neuroendocrine cells. Cellular and Molecular Life Sciences, 80(3), 79.
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Publication 2023
Acetaminophen Bronchi Bronchus, Primary Cell Nucleus DAPI Epithelium Fluorescence Genotype Lung Mus Neuroendocrine Cells SOX2 protein, human SOX21 protein, human
5
Evaluation of SARS-CoV-2 Antivirals in Rhesus Macaques
Male and female rhesus macaques (2–12 years of age) were obtained from the colony on Morgan Island, South Carolina, owned by the NIH National Institute of Allergy and Infectious Diseases (NIAID). The animals were divided into vehicle (n = 5) or treatment (n = 5 for MK-4482, n = 5 for PF-07321332, and n = 5 for the MK-4482/PF-07321332 combination) groups prior to infection. MK-4482 (DC Chemicals), PF-07321332 (DC Chemicals), and ritonavir (DC Chemicals) were first dissolved in DMSO and then resuspended to 5 mL total volume in food-grade peanut oil for oral delivery. Treated rhesus macaques received 130 mg/kg MK-4482, 20 mg/kg PF-07321332 + 6.5 mg/kg ritonavir, or the combination of all 3 compounds (130 mg/kg MK-4482 + 20 mg/kg PF-07321332 + 6.5 mg ritonavir) every 12 hours beginning 12 hours postinfection (hpi) and ending 84 hpi. Vehicle controls received similar volumes of peanut oil (5 mL) on the same schedule. Animals were infected with a total of 2 × 106 TCID50 of the SARS-CoV-2 Delta VOC by intranasal and intratracheal routes. Intranasal inoculation consisted of a 0.5 mL injection directly into each nare (1.0 mL total) with a Mucosal Atomization Device system (Teleflex). Intratracheal inoculations were performed with the use of a bronchoscope for deposition of 4 mL of virus directly into the main stem bronchi. All procedures were performed on anesthetized animals. Animals were monitored twice daily and scored blindly every morning by the same person for disease signs and progression as previously reported (19 (link)). Clinical exams were performed prior to challenge and at 0, 1, 2, and 4 dpi. Oropharyngeal, nasal, and rectal swabs, as well as whole blood and serum, were collected at every exam. BAL samples were collected at 1, 2, and 4 dpi. Animals were euthanized on 4 dpi and tissues were collected at necropsy.
Rosenke K., Lewis M.C., Feldmann F., Bohrnsen E., Schwarz B., Okumura A., Bohler W.F., Callison J., Shaia C., Bosio C.M., Lovaglio J., Saturday G., Jarvis M.A, & Feldmann H. (2023). Combined molnupiravir-nirmatrelvir treatment improves the inhibitory effect on SARS-CoV-2 in macaques. JCI Insight, 8(4), e166485.
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Publication 2023
Animals Autopsy BLOOD Bronchoscopes Bronchus, Primary Disease Progression Food Infection Macaca mulatta Males Medical Devices MK-4482 Mucous Membrane Nose Obstetric Delivery Oropharynxs Peanut Oil PF-07321332 Rectum Ritonavir SARS-CoV-2 Serum Stem, Plant Sulfoxide, Dimethyl Tissues Vaccination Virus Woman

Top products related to «Bronchus, Primary»

1
Bronchial epithelial growth medium (begm) by Lonza
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BEGM is a basal medium developed for the in vitro culture of normal human bronchial epithelial cells. It provides the essential nutrients and growth factors required for the optimal growth and differentiation of these cells in culture.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Cc 2540 by Lonza
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The CC-2540 is a specialized laboratory equipment designed for cell culture applications. It serves as a reliable incubator for maintaining optimal environmental conditions for cell growth and proliferation. The core function of the CC-2540 is to provide a controlled temperature, humidity, and gas composition within the incubation chamber.
6
Beas 2b by American Type Culture Collection
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The BEAS-2B cell line is a human bronchial epithelial cell line derived from normal human bronchial epithelial cells. It is commonly used in cell biology research and drug discovery applications.
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Begm bullet kit by Lonza
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The BEGM Bullet Kit is a set of laboratory equipment designed for cell culture applications. It includes essential components required for the growth and maintenance of basal epithelial cells in vitro.
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Fetal bovine serum (fbs) by Merck Group
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FBS, or Fetal Bovine Serum, is a commonly used cell culture supplement. It is derived from the blood of bovine fetuses and provides essential growth factors, hormones, and other nutrients to support the growth and proliferation of a wide range of cell types in vitro.
9
Pneumacult ali medium by STEMCELL
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PneumaCult-ALI medium is a cell culture medium designed to support the growth and differentiation of airway epithelial cells in an air-liquid interface (ALI) culture system. The medium is optimized to maintain the phenotypic characteristics and functional properties of these cells.
10
Transwell insert by Corning
Sourced in United States, United Kingdom, Germany, China, Switzerland, Japan, France, Spain
Transwell inserts are a type of laboratory equipment used for cell culture applications. They consist of a porous membrane that separates two chambers, allowing for the study of interactions between cells or the passage of substances across the membrane. The core function of Transwell inserts is to facilitate the creation of a barrier between the two chambers, enabling researchers to analyze various cellular processes and transport mechanisms.

More about "Bronchus, Primary"

The primary bronchus, also known as the principal bronchus, is a crucial component of the respiratory system.
It is the main airway that branches off from the trachea and leads to the lungs, dividing into the left and right primary bronchi, which then branch further into smaller secondary and tertiary bronchi.
Understanding the anatomy and function of the primary bronchus is essential for researchers and scientists studying respiratory health, disease, and interventions.
Primary bronchi play a vital role in facilitating the passage of air into and out of the lungs.
They are responsible for transporting oxygen-rich air into the lungs and removing carbon dioxide from the body.
Researchers often use various cell culture models and media to study the primary bronchus and its associated cellular processes, such as Bronchial Epithelial Growth Medium (BEGM), Fetal Bovine Serum (FBS), Penicillin/Streptomycin, and Dulbecco's Modified Eagle Medium (DMEM).
Cell lines like CC-2540 and BEAS-2B, which are derived from human bronchial epithelial cells, are commonly used in primary bronchus research.
The BEGM Bullet Kit and PneumaCult-ALI medium are also valuable tools for culturing and maintaining bronchial epithelial cells in vitro.
Transwell inserts can be used to create air-liquid interface (ALI) cultures, which mimic the natural environment of the primary bronchus.
PubCompare.ai's innovative AI-driven comparisons can help optimize research protocols for reproducibility and accuracy, allowing you to discover the best protocols and products by comparing data from literature, preprints, and patents.
This cutting-edge tool can streamline your research process and enhance the quality of your findings, leading to a better understanding of the primary bronchus and its role in respiratory health and disease.