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Buccal Mucosa

The buccal mucosa is the lining of the inside of the cheeks and lips.
It is a specialized type of mucous membrane that facilitates chewing, speaking, and swallowing.
This area is an important site for drug delivery and research, as the buccal mucosa is highly vascularized and permeable, allowing for rapid absorption of medications.
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Most cited protocols related to «Buccal Mucosa»

1
Taizhou Longitudinal Health Study
The TZL study was initiated by Fudan University and the Taizhou government in 2007, and "starting seed funding" came from the latter. The TZL study aims to recruit at least 100,000 adults aged 30–80 years from the general population of Taizhou. A three-stage stratified sampling method will be used.
In stage I, one out of three subdistricts (comprising several communities) or towns (comprising several rural communities) will be sampled from Hailing and Gaogang, representing the geographic and economic characteristics in their regions. In stage II, 50% of communities will be randomly selected from each subdistrict or town. In the third stage of sampling, all individuals aged 30–80 years from each household will be chosen.
The TZL study includes a two-phase baseline survey. In phase I, about 100,000 adults aged 30–80 years will be interviewed through questionnaires and samples of buccal mucosal cells will be collected in the communities mentioned above. In phase II, fasting blood samples will be collected for biochemical measurements (e.g., lipid, glucose, hepatic function, renal function) in half of the communities from phase I. After baseline investigation, continuous monitoring of morbidity and mortality will be conducted through a chronic disease register system. Follow-ups will be conducted every three years for event endpoint (see figure 1). The study will use a 'nested' case-control approach for studies on serological markers or genetic biomarkers if sufficient numbers of subjects have developed or died from the particular diseases of interest. By seeking differences in biomarkers in stored DNA and plasma, a wide range of genetic and environmental correlates and causes can be studied. Such an approach allows many factors to be studied in relation to many diseases at relatively low cost.
Wang X., Lu M., Qian J., Yang Y., Li S., Lu D., Yu S., Meng W., Ye W, & Jin L. (2009). Rationales, design and recruitment of the Taizhou Longitudinal Study. BMC Public Health, 9, 223.
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Publication 2009
Adult Biological Markers BLOOD Buccal Mucosa Cells Disease, Chronic Genes, vif Genetic Markers Glucose Households Kidney Lipids Plasma Rural Communities
2
Rat Model of Temporalis Tendon Injury
Male Sprague-Dawley rats, ≈ 8-week old at the time of surgery, were used (225-250 g, Harlan, Indianapolis, IN). Ligation of the TASM was achieved via an intraoral approach. Animals were anesthetized with pentobarbital sodium (50 mg/kg i.p.). The rat was fixed on a table and the head was supine. The mouth of the rat was kept open with a retractor. On the left side, a three-mm long incision was made posterior-anteriorly along but just lateral to the gingivobuccal margin in the buccal mucosa, beginning immediately next to the first molar. The TASM was gently freed from surrounding connective tissues and clearly visualized. The tendon was tied with two chromic gut (4.0) ligatures, 2-mm apart. The wound was checked for hemostasis and the incision closed with three 4.0 silk sutures. The sham-operated rats received the same procedure except that the tendon was not ligated. Changes in gross behavior and body weight in operated rats were monitored and compared with that in naive rats. Standard hematoxylin and eosin (EMS, Hatfield, PA) staining was performed to examine histological changes of the TASM after ligation, including infiltration of immune cells and morphology of the tendon tissue. For time course of hyperalgesia, 14 rats were used for the TASM ligation and sham groups, respectively. For local anesthesia and analgesic drugs experiments, 5 rats were used for each experimental group. For immunohistochemistry and western blot experiments, 3-4 rats were used for each group. All experiments were carried out in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80-23) and approved by the University of Maryland Dental School Institutional Animal Care and Use Committee. All efforts were made to minimize the number of animals used and their suffering.
Guo W., Wang H., Zou S., Wei F., Dubner R, & Ren K. (2010). Long lasting pain hypersensitivity following ligation of the tendon of the masseter muscle in rats: A model of myogenic orofacial pain. Molecular Pain, 6, 40.
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Publication 2010
Analgesics Animals Animals, Laboratory Body Weight Buccal Mucosa Cells Connective Tissue Eosin Head Hematoxylin Hemostasis Hyperalgesia Immunohistochemistry Institutional Animal Care and Use Committees Ligation Ligature Local Anesthesia Males Molar Oral Cavity Pentobarbital Sodium Rats, Sprague-Dawley Rattus norvegicus Silk Sutures Tendons Tissues Western Blot Wounds
3
16S rRNA Amplicon Sequencing of Sponge and Oral Microbiomes
We constructed amplicon libraries from sponge samples that span the V4-V5 16S rRNA region (Supplementary Methods). Supplementary Table S1 describes the 16S-specific primers and the sequencing adaptors for paired-end sequencing on the Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA) using 2 × 250 cycles. V3-V5 pyrosequencing reads (250 nt in length) from a publicly available oral microbiome study (The Human Microbiome Project Consortium, 2012b (link)) represent samples from nine sites in the human mouth and pharynx (subgingival plaque, supragingival plaque, buccal mucosa, keratinized gingiva, tongue dorsum, hard palate, saliva, palatine tonsils and throat).
Eren A.M., Morrison H.G., Lescault P.J., Reveillaud J., Vineis J.H, & Sogin M.L. (2014). Minimum entropy decomposition: Unsupervised oligotyping for sensitive partitioning of high-throughput marker gene sequences. The ISME Journal, 9(4), 968-979.
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Publication 2014
Buccal Mucosa Dental Plaque Gingiva Hard Palate Homo sapiens Human Microbiome Microbiome Oligonucleotide Primers Oral Cavity Palatine Tonsil Pharynx Porifera RNA, Ribosomal, 16S Saliva Tongue
4
Protein Extraction from Mouse Tissues
Normal buccal mucosa and tongue from 5 pairs of Tgfbr1f/f/Ptenf/f and Tgfbr1/Pten 2cKO mice, together with tumors that developed in Tgfbr1/Pten 2cKO mice, were carefully dissected. A total of 40 μg of protein from each sample were denatured and then loaded in each lane of NuPAGE 4-12% Bis-Tris precast gel. Additional details are provided in the Supplementary Data.
Bian Y., Hall B., Sun Z.J., Molinolo A., Chen W., Gutkind J.S., Van Waes C, & Kulkarni A.B. (2011). Loss of TGF-β Signaling and PTEN Promotes Head and Neck Squamous Cell Carcinoma through Cellular Senescence Evasion and Cancer-related Inflammation. Oncogene, 31(28), 3322-3332.
Publication 2011
Bistris Buccal Mucosa Mus Neoplasms PTEN protein, human Staphylococcal Protein A TGFBR1 protein, human Tongue
5
Chronic Constriction Injury of the Infraorbital Nerve

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Publication 2014
Animals Buccal Mucosa Injuries Ligature Males Mice, House Mice, Inbred C57BL Nervousness Pentobarbital Sodium Stenosis Tissues

Most recents protocols related to «Buccal Mucosa»

1
Buccal Mucosa Sampling for Metabolomic Analysis
Before sample collection, a dentist confirmed that at least 2 h had passed since the previous meal. After removing visible food residue, samples were collected from the buccal mucosa using a Hummingood sponge brush (Molten Corporation, Hiroshima, Japan), which had been dipped into 5 mL saline in a 50 mL tube and squeezed briefly onto the side of the tube. The samples were collected by placing the brush on the buccal mucosa, rubbing “back and forth” 10 times at a rate of 1 rub/second. The sponge was returned to the saline-containing tube, pressed, and squeezed tightly. After collection, the samples were stored at − 20 °C, thawed, and centrifuged at 3,000 × g for 5 min at 4 °C. The supernatants were used for further analysis by LC–MS/MS and multiplex assays.
Ogura K., Endo M., Hase T., Negami H., Tsuchiya K., Nishiuchi T., Suzuki T., Ogai K., Sanada H., Okamoto S, & Sugama J. (2023). Potential biomarker proteins for aspiration pneumonia detected by shotgun proteomics using buccal mucosa samples: a cross-sectional case–control study. Clinical Proteomics, 20, 9.
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Publication 2023
Biological Assay Buccal Mucosa Dentist Food Porifera Saline Solution Tandem Mass Spectrometry
2
Oral Microbiome Sampling Techniques
Samples were taken two times, first within 36 h of intubation (baseline) and again after 7 days of intubation (follow-up).
In each sampling, four oral habitats were sampled: buccal mucosa, tongue, buccal dental surface and gingival pocket. Every sample was taken by a trained dentist.
Sampling the posterior part of the dorsum of the tongue and buccal mucosa, we used ESwab™. ESwab combines a COPAN-invented flocked swab with 1 mL of liquid amine in a plastic, screw cap tube. Dental plaque was collected from the buccal dental surface side using Tooth Cleanic KerrHawe—KWX-OP-SZ-011, and after collection, the brush was placed in 1 mL of Liquid Amies in a plastic screw cap tube. Three pieces of PerioPaper Strips, designed to absorb or carry 0–1.2 µl of fluid, were used to collect gingival cerficular fluid (GCF) samples. The strips were placed in the gingival pocket for 30–45 s until its surface soaked up. To minimize the risk of preanalytical errors during sample collection, sterile gauze was used to remove excess saliva from the mucosae and dry the dental surfaces, preventing salivary contamination of GCF.
Gregorczyk-Maga I., Pałka A., Fiema M., Kania M., Kujawska A., Maga P., Jachowicz-Matczak E., Romaniszyn D., Chmielarczyk A., Żółtowska B, & Wójkowska-Mach J. (2023). Impact of tooth brushing on oral bacteriota and health care-associated infections among ventilated COVID-19 patients: an intervention study. Antimicrobial Resistance and Infection Control, 12, 17.
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Publication 2023
Amines Buccal Mucosa Dental Health Services Dental Plaque Dentist Gingiva Gingival Pocket Intubation Mucous Membrane Saliva Specimen Collection Sterility, Reproductive Tongue Tooth
3
Oral Pathology Retrospective Analysis
The biopsy records and respective anatomopathological reports issued between 1997 and 2021 were obtained from the Oral Pathology Laboratory of the University of Sao Paulo School of Dentistry. In addition, demographic data on the patient were collected, including information on the lesion (i.e. site, evolution time), biopsy type (incisional or excisional), diagnostic hypotheses (benign or malignant lesion) and final diagnosis given by the pathologists.
The final diagnoses were established according to the 2017 World Health Organization classification of head and neck tumours which was used in the studies by Da Silva et al. (2019) (4 (link)), Silva et al. (2018) (9 (link)). De Carvalho et al. (2020) (10 (link)), and De Arruda et al. (2017) (13 (link)). The patients were divided into three age groups as follows: 0-3 years old (babies and toddlers), 4-11 years old (children) and 12-18 years old (adolescents). The anatomical sites involved were classified into maxilla, mandible, palate, jugal mucosa, lips, gingiva, tongue, oropharynx and others.
At the moment of this study, all the histological slides of malignant neoplasms were reviewed by two oral pathologists, except for one case (#7) of a diagnosis of ganglioneuroblastoma whose slide and paraffin block were not found in the records.The collected data were tabulated in electronic spreadsheet (Excel® Microsoft) for descriptive and quantitative analysis by using the Open Source Jamovi® software, version 1.6.23.
da Cunha W.A., Corazza A.C., Rezende K.M., Bönecker M, & Gallottini M. (2023). Paediatric head and neck malignant neoplasms: A brazilian retrospective study. Medicina Oral, Patología Oral y Cirugía Bucal, 28(2), e140-e147.
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Publication 2023
Adolescent Age Groups Biological Evolution Biopsy Body Regions Buccal Mucosa Child Diagnosis Ganglioneuroblastoma Gingiva Head and Neck Neoplasms Infant Lip Malignant Neoplasms Mandible Maxilla Oropharynxs Palate Paraffin Pathologists Patients Tongue
4
Induction of Periodontal Disease in Rats
The treatment and care of the animals was done in accordance with the regulations set forth by the Cairo Agriculture for Experimental Animals’ Animal Ethics Committee, Cairo, Egypt, Approval No (113-10-22). According to the Declaration of Helsinki and its Guiding Principles for the Care and Use of Animals (NIH Publication No. 85-23, 1985 revision), researchers adhered to certain rules. The experimental rats were housed in cages in the laboratory with free access to food and water. In order to reduce suffering, proper care was given to the animals. Prior to the experiment, the animals were acclimated for at least 14 days under typical circumstances of 25 °C temperature and 55.5% relative humidity with a 12-hour cycle of light and darkness. The experiment involved 48 adult rats, which were divided into 16 groups of 3 rats each. Every group received treatment using a formulation chosen based on the study design. The following steps were taken to construct the in-vivo periodontitis model. Chloral hydrate 10% was injected intraperitoneally to anesthetize the rats. After that, they were secured in the supine posture, and a homemade mouth expander was used to open the mouth. A gingival separator was used to separate the gums and totally expose the bilateral maxillary first molars. Each rat in the group had both of their maxillary first teeth tied together for a period of 2 weeks using an orthodontic steel wire (0.2 mm in diameter). By placing the ligation wires in the gingival sulcus, the ligation did not interfere with the rats’ ability to feed or harm their buccal mucosa. In other groups, the periodontitis model was developed in a single day. The buccal, lingual, and mesial gingiva of the maxillary molars, as well as the adjacent tooth space between the maxillary molars, were then injected with variously produced CrO-Tur-SNEDDS (60 µl for each tooth).
Sindi A.M., Hosny K.M., Rizg W.Y., Sabei F.Y., Madkhali O.A., Bakkari M.A., Alfayez E., Alkharobi H., Alghamdi S.A., Banjar A.A., Majrashi M, & Alissa M. (2023). Utilization of experimental design in the formulation and optimization of hyaluronic acid–based nanoemulgel loaded with a turmeric–curry leaf oil nanoemulsion for gingivitis. Drug Delivery, 30(1), 2184311.
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Publication 2023
Adult Animal Ethics Committees Animals Buccal Mucosa Darkness Food Gingiva Humidity Hydrate, Chloral Ligation Maxilla Molar Oral Cavity Orthodontic Wires Periodontitis Rattus norvegicus Steel Tongue Tooth
5
Ex-vivo Permeation of Tur Formulations
Ex-vivo tests were performed on the Tur aqueous suspension and various manufactured formulations (Xiang et al., 2002 (link)), each of which included 37.5 mg/ml of Tur. An automatic Franz diffusing cell (MicroettePlus, Paso Robles, CA, USA) was used as the apparatus, and sheep buccal mucosa from a nearby slaughterhouse was used as the model permeation membrane. The Franz diffusion cell’s donor and receptor chambers were properly positioned to encompass the processed sheep buccal mucosa (2 × 2 cm) (1.75 cm2). The medium was agitated at a rate of 400 to 450 rpm while being kept at a temperature of 37 ± 0.5 °C in the receptor chamber, which contained 8 ml of PBS (pH 6.8). At regular intervals, finite aliquots were automatically taken, and the aforementioned HPLC technique was used to quantify the Tur content. Plotting the cumulative amount of permeated Tur (Q24) per unit of area against time let researchers better understand how the drug was dispersed across the mounted mucosa. From the acquired diffusion data, significant parameters such as the Jss (steady-state flow), Pc (permeability coefficient), EF (enhancement factor), and D (diffusion coefficient) were determined. Plots were made of the comparative permeation patterns for various formulations. The following equation as used to determine the percentage of permeated Tur and the overall amount of Tur distributed across the receptor chamber (Hosny et al., 2021 (link)):
Percentage permeatedTpTi×100
where Ti was the initial quantity of Tur in the donor compartment and Tp was the amount of Tur that permeated the receptor compartment.
Sindi A.M., Hosny K.M., Rizg W.Y., Sabei F.Y., Madkhali O.A., Bakkari M.A., Alfayez E., Alkharobi H., Alghamdi S.A., Banjar A.A., Majrashi M, & Alissa M. (2023). Utilization of experimental design in the formulation and optimization of hyaluronic acid–based nanoemulgel loaded with a turmeric–curry leaf oil nanoemulsion for gingivitis. Drug Delivery, 30(1), 2184311.
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Publication 2023
Buccal Mucosa Cells Diffusion Domestic Sheep High-Performance Liquid Chromatographies Mucous Membrane Permeability Pharmaceutical Preparations Tissue, Membrane Tissue Donors

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Coreexom psychchip by Illumina
The CoreExom PsychChip is a microarray-based genetic analysis tool designed for the detection and analysis of genetic variants associated with psychiatric and neurological disorders. It provides comprehensive coverage of genomic regions and genetic markers relevant to the study of these conditions.
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More about "Buccal Mucosa"

Buccal Mucosa, also known as the oral mucosa or oromucosal membrane, is the lining of the inside of the cheeks and lips.
It is a specialized type of mucous membrane that plays a crucial role in various physiological functions, such as chewing, speaking, and swallowing.
This area is of great interest in the field of drug delivery research and development, as the buccal mucosa is highly vascularized and permeable, allowing for rapid absorption of medications.
One of the key advantages of the buccal mucosa for drug delivery is its accessibility, as it is easily accessible for both topical and systemic administration of pharmaceuticals.
Furthermore, the buccal mucosa avoids first-pass metabolism, which can lead to increased bioavailability of drugs.
Researchers often utilize various techniques and tools to study the buccal mucosa and its potential for drug delivery.
Some commonly used tools and techniques include the QIAamp DNA Mini Kit for DNA extraction, the CoreExom PsychChip for genetic analysis, the QIAamp DNA Blood Mini Kit for DNA extraction from blood samples, the TaqMan 5′ exonuclease assay for gene expression analysis, and the MassARRAY system for high-throughput genotyping.
Additionally, cell culture models, such as those utilizing Dulbecco's Modified Eagle Medium (DMEM) and Fetal Bovine Serum (FBS), are often employed to study the buccal mucosa in vitro.
The NanoDrop 2000c spectrophotometer is a useful tool for quantifying and assessing the purity of nucleic acids extracted from buccal mucosa samples.
Researchers are constantly exploring new ways to optimize buccal mucosa research, and platforms like PubCompare.ai's AI-driven platform can be valuable resources.
These platforms can help researchers easily locate relevant protocols from the literature, pre-prints, and patents, and leverage AI-powered comparisons to identify the best protocols and products for their specific needs, ultimately enhancing the reproducibility and accuracy of their buccal mucosa research.