Animal behavior was monitored in the head/chest region and the torso/arm/leg region using two video cameras (MTC-9272, Mother Tool, Ueda, Japan; WAT-232S, Watec, Tsuruoka, Japan). Videos were stored in a multi-channel video recorder (RD4304, ARUCOM, Fukuoka, Japan). Stored movies were viewed offline with video software (ifileplaypack, ARUCOM). We noted the following convulsive or voluntary movements, and their timing and frequency were analyzed by an observer familiar with monkey behavior: “Twitch” (a sudden jerk of the hand/arm or leg), “Tremor” (continuous and rhythmical shaking of the hand/arm that lasted for longer than 2 s), clonic seizure in the “Body” (large scale shaking of the body, including torso and legs), clonic seizure in the “Head” (whole-face movements ranging from small twitches in the unilateral lip and/or eyebrows to large scale jerking), “Voluntary movements” (non-periodical large movement of the arms and/or body).
Miyakawa N., Nagai Y., Hori Y., Mimura K., Orihara A., Oyama K., Matsuo T., Inoue K.I., Suzuki T., Hirabayashi T., Suhara T., Takada M., Higuchi M., Kawasaki K, & Minamimoto T. (2023). Chemogenetic attenuation of cortical seizures in nonhuman primates. Nature Communications, 14, 971.