A ratiometric GCAMP3-based calcium sensor was expressed in cilia to measure ciliary calcium in hRPE1 cells. A transgenic mouse model expressing GFP in cilia was generated to measure ciliary calcium and membrane potential by patch clamp recordings of cilia. Defects in Shh signaling of MEF isolated from PKD2-L1-/- mutant mice were quantified by Western blotting and ciliary localization of Gli1 protein.
Eyelashes
Eyelashes are the specialized hairs that grow on the edges of the eyelids, providing a protective barrier and enhancing the appearance of the eyes.
They play a crucial role in vision, helping to prevent debris and foreign particles from entering the eyes.
Eyelashes also contribute to the overall aesthetics of the face, with their length, thickness, and curl being important factors in beauty and self-expression.
Research on eyelashes covers a wide range of topics, from the biology and development of these structures to their clinical applications in ophthalmology and cosmetic procedures.
PubCompare.ai's AI-driven platform can help researchers optimize their studies on eyelashes, locating the best protocols from the literature, pre-prints, and patents, and enabling reproducibility and accuracy in their work.
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They play a crucial role in vision, helping to prevent debris and foreign particles from entering the eyes.
Eyelashes also contribute to the overall aesthetics of the face, with their length, thickness, and curl being important factors in beauty and self-expression.
Research on eyelashes covers a wide range of topics, from the biology and development of these structures to their clinical applications in ophthalmology and cosmetic procedures.
PubCompare.ai's AI-driven platform can help researchers optimize their studies on eyelashes, locating the best protocols from the literature, pre-prints, and patents, and enabling reproducibility and accuracy in their work.
Experienece the future of research optimization with PubCompare.ai.
Most cited protocols related to «Eyelashes»
Calcium
Cells
Cilia
Eyelashes
GLI1 protein, human
Membrane Potentials
Mice, Laboratory
Mice, Transgenic
5'-N-methylcarboxamideadenosine
Animals
Apoptosis
Bacteria
Blood
Bronchoalveolar Lavage
Cells
DNA, Bacterial
Eyelashes
Flow Cytometry
Head
Immune Sera
Institutional Animal Care and Use Committees
Leukocytes
Light Microscopy
Lung
Macrophage
Middle Ear
MUC5AC protein, human
MUC5B protein, human
Mus
neuro-oncological ventral antigen 2, human
Neutrophil
Otitis Media
Otoscopy
Oxygen
Pellets, Drug
Plethysmography
Pneumonia
prisma
Proteins
Pulse Rate
Rabbits
Respiratory Physiology
RNA, Ribosomal, 16S
Staphylococcus aureus
Adrenal Cortex Hormones
Adult
Antibiotics
Aspirin
Asthma
Conditioning, Psychology
Cystic Fibrosis
Diagnosis
Endoscopy
Ethics Committees, Research
Ethnicity
Eyelashes
Gender
Head
Hypersensitivity
Nasal Polyps
Nasal Sprays
Neck
North American People
Operative Surgical Procedures
Otorhinolaryngologic Surgical Procedures
Patients
Physical Examination
Sinuses, Nasal
Sinusitis
Skin
Sleep Apnea, Obstructive
Steroids
Therapeutics
X-Ray Computed Tomography
Animals
Edema
Eyelashes
Forehead
Gossypium
Lens, Crystalline
Lubricant Eye Drops
Neoplasm Metastasis
Nose
Saline Solution
Tears
Viscosity
The diagnostic criteria used in the UK (UHS and RBH) have previously been described in detail in Jackson et al. [24 ] and Lucas and Leigh [17 (link)]. In brief, a positive diagnosis is usually based on a typical clinical history with at least two abnormal diagnostic tests (“hallmark” transmission electron microscopy (TEM), “hallmark” ciliary beat pattern (CBP), nNO ≤30 nL·min−1). Occasionally patients with a strong history (e.g. sibling with PCD, “full” clinical phenotype (e.g. neonatal respiratory distress at term followed by daily wet cough, persistent rhinitis and glue ear)), are diagnosed based on either “hallmark” TEM or repeated high-speed video microscopy analysis (HSVMA) consistent with PCD. CBP was only considered positive if the pattern was typical of PCD rather than secondary ciliary dyskinesia either from two brushing biopsies or from one brushing biopsy with reanalysis following air–liquid interface culture.
Biopsy
Ciliary Motility Disorders
Cough
Eyelashes
Infant, Newborn
Microscopy, Video
Otitis Media with Effusion
Patients
Phenotype
Respiratory Rate
Rhinitis
Tests, Diagnostic
Transmission Electron Microscopy
Most recents protocols related to «Eyelashes»
U12 genes were identified by running the U12db (22 (link)) annotation pipeline on the GRCh38 version of the human genome, with the gencode38 version of the annotations, or the GRCz11 version of the zebrafish genome. GO term analysis was performed with the TopGO (v2.38.1) R tool (37 (link)), with the default “weight01” algorithm, the Fisher-test and the org.Hs.eg.db (v3.10.0) R database of the genome-wide annotation for humans. Genes without any GO annotation were discarded.
To constitute a list of cilium-related genes, we combined three existing databases and defined a gene as cilium-related if 1) it was part of the Gene_Ontology (GO = Cilium) of the Gold_Standard (SysCilia, a curated list of known ciliary components) (17 (link)) or of the Predicted candidates based on a bayesian integration in CiliaCarta (38 (link)) (last updated version of March, 18th 2018); 2) it was in the CilDB table v3.0 and had the maximum human ciliary evidence stringency level and at least 3 ciliary evidence (39 (link), 40 (link)) (last update June 2014); and 3) it was a ciliopathy-related gene (18 (link)–20 (link, link)).
To constitute a list of cilium-related genes, we combined three existing databases and defined a gene as cilium-related if 1) it was part of the Gene_Ontology (GO = Cilium) of the Gold_Standard (SysCilia, a curated list of known ciliary components) (17 (link)) or of the Predicted candidates based on a bayesian integration in CiliaCarta (38 (link)) (last updated version of March, 18th 2018); 2) it was in the CilDB table v3.0 and had the maximum human ciliary evidence stringency level and at least 3 ciliary evidence (39 (link), 40 (link)) (last update June 2014); and 3) it was a ciliopathy-related gene (18 (link)–
Cilia
Ciliopathies
Eyelashes
Gene Annotation
Genes
Genes, vif
Genome
Genome, Human
Gold
Homo sapiens
Zebrafish
Dechorionated embryos were washed in PBS, fixed in 4% PFA overnight prior to yolk removal. After 3-h incubation in blocking solution (PBS+0.1% Tween20 (PBST), 3% BSA), embryos were washed thrice in PBST and incubated overnight at 4 °C with anti-acetylated α-tubulin antibody in PBST (1:500, T6793, Sigma). Embryos were then extensively washed and incubated with Phalloidin–tetramethylrohdamine B isothiocyanate (1:100, P1951, Sigma) for 20 min at room temperature, before being washed and incubated again overnight at 4 °C with anti-mouse secondary antibody in PBST (1:2,000, A32728, Invitrogen). Finally, embryos were mounted on glass slides using FluoPreserveTM Reagent (Merck) prior to confocal imaging using Zeiss LSM880 microscope. Live embryos were mounted in 0.4 % low-melting agarose mixed with Tricain in glass-bottom Petri dishes (Ibidi). Beating cilia were imaged by optimizing the signal-to-noise ratio and the scan speed, as previously described (43 (link)). Z-stacks were performed to acquire the whole organs and quantification of cilium number, and range of motion was done manually and blinded on images of maximum intensity projection of z-stacks, using ImageJ software.
alpha-Tubulin
Antibodies, Anti-Idiotypic
Cilia
Embryo
Eyelashes
Hyperostosis, Diffuse Idiopathic Skeletal
Isothiocyanates
Microscopy
Mus
Phalloidine
Radionuclide Imaging
Sepharose
Tween 20
A Zeiss LSM 700 microscope was used for imaging of IF samples. The pinhole was set to 1 Airy unit at maximal optical resolution before gain and offset were calibrated. Fluorescent signal intensities were within the linear range of detection. For all IF image analyses, ImageJ software was used. For high resolution IF, a 40x silicone immersion objective (numerical aperture: 1.25) was used at the Nikon AX confocal microscope. Pixel size was set to 100 nm and z sections were obtained at the ventral rim of the embryo to reduce background signal. Images were deconvolved using Nis-Elements 3D Deconvolution. Quantification of the ciliogenesis defects in X. tropicalis MCC cells was performed by immunostaining against acetylated tubulin and phalloidin to identify the ciliary axoneme and MCC border, respectively. MCC motile cilia were classified as ‘Normal’ or ‘Defect’ (showing lower cilia density and shorter cilia) as well as ‘Mild’ (>half the length compared to control), ‘Severe’ (X. tropicalis embryos were examined by calculating the ratio of MCC or Ionocytes to total cells. pLrp6 stainings as well as LRP6 WT, LRP6VA and LRP6(VA)P(PA) stainings were quantified by measuring their IF-positive areas and then normalizing to the AcTub+ area for each MCC using Fiji (ImageJ). Each data point represents one ratio. For the GSK3 ciliary biosensor live cell imaging, the mean signal intensity was measured for one MCC per experiment and time-point. The mean signal intensity for each MCC was normalized to that of the first time point (t = 0) in the representative graph. In fixed ciliary GSK3 biosensor-injected embryos, one full image per embryo was measured using Fiji (ImageJ). Ciliary GSK3 mean intensity was normalized to Arl13b-mKate2 mean intensity in the latter.
Images for phenotypical analyses were taken with the AxioCam MRc 5 microscope (Zeiss). Embryos were scored blind and data are representative images from 2 or more independent experiments. Representative embryos were selected using Magnetic Lasso tool of Adobe Photoshop CS6. Background was adjusted uniformly for presentation. Phenotypical features were analyzed by comparison to control embryos and classified and ‘normal’ and ‘defect’ (showing DV/AP patterning defects).
Images for phenotypical analyses were taken with the AxioCam MRc 5 microscope (Zeiss). Embryos were scored blind and data are representative images from 2 or more independent experiments. Representative embryos were selected using Magnetic Lasso tool of Adobe Photoshop CS6. Background was adjusted uniformly for presentation. Phenotypical features were analyzed by comparison to control embryos and classified and ‘normal’ and ‘defect’ (showing DV/AP patterning defects).
Full text: Click here
Axoneme
Biosensors
Blindness
Cells
Cilia
Embryo
Eyelashes
GART protein, human
Glycogen Synthase Kinase 3
LRP6 protein, human
Microscopy
Microscopy, Confocal
Motile Cilia
Phalloidine
Silicones
Staining
Submersion
Tubulin
Vision
The deciliation procedure was adapted from Werner and Mitchell, 201332 (link). Embryos were cultured until St. 30. Between 70 and 100 embryos were deciliated in 75 mM CaCl2, 0.02% NP-40 (Sigma) in 1/19 MR for 2 min or until no cilia driven movements were observed, and then the buffer containing motile cilia was centrifuged (15.000 g, 7 min). The cilia pellet was reconstituted in 20 µl of NOP + buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2% Triton X-100, 0.2% DTT, and cOmplete Protease Inhibitor Cocktail (Roche; Cat#11697498001)). NuPAGE LDS buffer (Thermo Scientific; Cat#NP0007) (+50 mM DTT) was added in a 1:4 ratio and samples were heated at 70 °C for 10 min before immunoblotting.
Full text: Click here
Buffers
Embryo
Eyelashes
Motile Cilia
Movement
Nonidet P-40
Protease Inhibitors
Sodium Chloride
Triton X-100
Tromethamine
For functionality testing of the implanted microstents, OPT was applied as an existing method to quantify retinal and ciliary blood pressures as well as perfusion pressures in humans.26 (link),27 (link) A suction cup oculopressor (Fa. B. Boucke, Medizin-Elektronik, Tübingen, Germany) approved in human medicine, consisting of a vacuum pump that is connected to the eyeball by a flexible tube, was used. The suction cup was adjusted to the anatomy of the rabbit eye as described elsewhere.28 Briefly, the suction cup oculopressor was used to increase the IOP about 40 mm Hg during an examination period of eight minutes. Before IOP increase using a suction cup oculopressor, the initial baseline IOP was measured. During OPT, the IOP was measured using the rebound tonometer (Fig. 3 ). To record pressure decay during OPT, the IOP was measured every minute. Finally, IOP was recorded eight minutes after termination of OPT. Upon completion of a series of measurements a moisturizing gel (Vidisic; Bausch & Lomb GmbH, Berlin, Germany) was applied to the eyes. In total, OPT was performed under general anesthesia as already described above, at 4 weeks postoperatively and subsequently every 4 weeks until the end of the study.
Eye
General Anesthesia
Homo sapiens
Perfusion
Pharmaceutical Preparations
Pressure
Rabbits
Retina
Suction Drainage
Vacuum
vidisic
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
More about "Eyelashes"
Eyelashes are the specialized hairs that line the edges of the eyelids, providing a protective barrier and enhancing the aesthetic appeal of the eyes.
These delicate structures play a crucial role in vision, helping to ward off debris and foreign particles from entering the ocular region.
Research on eyelashes encompasses a broad spectrum of topics, from the biological mechanisms governing their development and growth patterns to their clinical applications in ophthalmology and cosmetic procedures.
Utilizing advanced imaging techniques, such as inverted microscopy and confocal laser scanning microscopy (LSM 880) with Zen Black software, researchers can delve into the intricate morphology and ultrastructure of eyelashes.
The length, thickness, and curl of eyelashes are not only important factors in beauty and self-expression, but also hold significance in various medical and cosmetic interventions.
For instance, eyelash serums containing active ingredients like prostaglandin analogues (e.g., Latanoprost) or growth factors have been developed to enhance eyelash appearance and health.
In the realm of cell culture and tissue engineering, the use of DMEM (Dulbecco's Modified Eagle Medium) supplemented with penicillin/streptomycin has enabled researchers to study the underlying mechanisms of eyelash growth and differentiation.
Specialized software, such as MetaMorph, has also been employed to quantify and analyze eyelash characteristics, facilitating advancements in both scientific research and clinical applications.
By leveraging the power of AI-driven platforms like PubCompare.ai, researchers can optimize their studies on eyelashes, accessing the best protocols from literature, pre-prints, and patents.
This empowers them to enhance the reproducibility and accuracy of their work, ultimately leading to breakthroughs in understanding the complex biology and clinical relevance of these fascinating structures.
These delicate structures play a crucial role in vision, helping to ward off debris and foreign particles from entering the ocular region.
Research on eyelashes encompasses a broad spectrum of topics, from the biological mechanisms governing their development and growth patterns to their clinical applications in ophthalmology and cosmetic procedures.
Utilizing advanced imaging techniques, such as inverted microscopy and confocal laser scanning microscopy (LSM 880) with Zen Black software, researchers can delve into the intricate morphology and ultrastructure of eyelashes.
The length, thickness, and curl of eyelashes are not only important factors in beauty and self-expression, but also hold significance in various medical and cosmetic interventions.
For instance, eyelash serums containing active ingredients like prostaglandin analogues (e.g., Latanoprost) or growth factors have been developed to enhance eyelash appearance and health.
In the realm of cell culture and tissue engineering, the use of DMEM (Dulbecco's Modified Eagle Medium) supplemented with penicillin/streptomycin has enabled researchers to study the underlying mechanisms of eyelash growth and differentiation.
Specialized software, such as MetaMorph, has also been employed to quantify and analyze eyelash characteristics, facilitating advancements in both scientific research and clinical applications.
By leveraging the power of AI-driven platforms like PubCompare.ai, researchers can optimize their studies on eyelashes, accessing the best protocols from literature, pre-prints, and patents.
This empowers them to enhance the reproducibility and accuracy of their work, ultimately leading to breakthroughs in understanding the complex biology and clinical relevance of these fascinating structures.