> Anatomy > Body Part > Fallopian Tubes

Fascia →

Fallopian Tubes

Fallopian Tubes: The hidden pathways of fertility.
Discover the secrets of these intricate structures that play a crucial role in human reproduction.
PubCompare.ai, your AI-powered platform, offers a seamless way to optimize your research protocols and identify the most effective products and procedures for your Fallopian Tube studies.
Effortlessly locate the best protocols from literature, pre-prints, and patents using our intelligent comparison tools.
Unleash the power of AI to improve your workflow and uncover the true potential of these remarkable anatomical features.
Explore the world of Fallopian Tubes with PubCompare.ai today!

Most cited protocols related to «Fallopian Tubes»

Transgenic Mice Generation via CRISPR

B6D2F1 female mice were superovulated and mated with B6D2F1 males, and fertilized eggs were collected from the oviduct. The pronuclear stage eggs were injected with pX330 plasmids, hCas9 mRNA, and sgRNAs at indicated concentrations. The eggs were cultivated in kSOM overnight then transferred into the oviducts of pseudopregnant ICR females.

Mashiko D., Fujihara Y., Satouh Y., Miyata H., Isotani A, & Ikawa M. (2013). Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Scientific Reports, 3, 3355.

Publication 2013

Eggs Fallopian Tubes Females Males Mice, House Oviducts Plasmids RNA, Messenger Zygote

Deconvolving Tumor Cell Purity from DNA Methylation

DNA methylation data for 489 high stage, high grade serous ovarian tumors and eight normal fallopian tube samples was obtained from http://tcga.cancer.gov/dataportal/. In addition, buffy coat samples from two female individuals were obtained. All data were generated with Illumina Infinium HumanMethylation 27 arrays, which interrogate 27,578 CpG sites located in proximity to the transcription start sites of 14,475 consensus coding sequencing in the NCBI Database (Genome Build 36). The level of DNA methylation at each probe was summarized with beta values ranging from 0 (unmethylated) to 1 (methylated) ^{58 (link)}.
The leukocyte methylation signature was derived as follows. Each probe was ranked by the difference in mean beta value in buffy coat and fallopian tube samples. We retained the 100 probes with the largest positive difference and the 100 with the largest negative difference between mean DNA methylation in normal fallopian tube tissues and peripheral blood leukocytes, designated BC and FT, (buffy coat and fallopian tube enriched, respectively). Let T_ik denote the beta value for probe k in tumor sample i. Let B_k denote the average beta value of buffy coat samples for each probe. Let T_k denote the minimum observed beta value across all tumor samples for the BC probes and the maximum for the FT probes. Denote by f_B the fraction of buffy coat components in the sample, then we have the following equation for each probe: T_ik = B_kf_B + T_k(l−f_B). Solving this equation for f_B gives: f_B = (T_ik − T_k)/(B_k − T_k). The values of f_B for each of the 200 probes in the signature were calculated and a kernel density estimate was obtained. The leukocyte signature was then calculated as the mode of this density estimate.

Carter S.L., Cibulskis K., Helman E., McKenna A., Shen H., Zack T., Laird P.W., Onofrio R.C., Winckler W., Weir B.A., Beroukhim R., Pellman D., Levine D.A., Lander E.S., Meyerson M, & Getz G. (2012). Absolute quantification of somatic DNA alterations in human cancer. Nature biotechnology, 30(5), 413-421.

Publication 2012

DNA Methylation Fallopian Tubes Females Genome Leukocytes Malignant Neoplasms Methylation Neoplasms Ovary Serous Neoplasms Tissues Transcription Initiation Site

Multicenter RCT on Unexplained Infertility

The Assessment of Multiple Intrauterine Gestations from Ovarian Stimulation (AMIGOS) clinical trial was conducted by the National Institute of Child Health and Human Development (NICHD) Cooperative Reproductive Medicine Network; the Collaborative Center for Statistics and Science at Yale University served as the data coordinating center. The trial was conducted at 12 clinical sites throughout the United States.
The trial design has been published previously.^{22 (link)} Briefly, this was a multicenter, randomized clinical trial involving 900 couples with unexplained infertility. Women were between 18 and 40 years of age with regular menses (nine or more cycles per year), had a normal uterine cavity with at least one patent fallopian tube, and had a male partner with a semen specimen of at least 5 million sperm per milliliter. This threshold for the sperm count was used because the protocol incorporated a sperm wash with intrauterine insemination. (World Health Organization [WHO] thresholds for normal sperm counts, which are higher, are based on intercourse rather than insemination.) In addition, a sperm wash was performed to concentrate motile sperm; this approach has been shown to improve pregnancy rates with sperm counts below the WHO threshold.^{23 (link)}Approval for the study was obtained from the institutional review board at each site. Written informed consent was provided by all female and male participants. The first and last authors assume responsibility for the accuracy and completeness of the data reporting and for the fidelity of the report to the study protocol, which is available with the full text of this article at NEJM.org.

Diamond M.P., Legro R.S., Coutifaris C., Alvero R., Robinson R.D., Casson P., Christman G.M., Ager J., Huang H., Hansen K.R., Baker V., Usadi R., Seungdamrong A., Bates G.W., Rosen R.M., Haisenleder D., Krawetz S.A., Barnhart K., Trussell J.C., Ohl D., Jin Y., Santoro N., Eisenberg E, & Zhang H. (2015). Letrozole, Gonadotropin, or Clomiphene for Unexplained Infertility. The New England journal of medicine, 373(13), 1230-1240.

Publication 2015

Coitus Dental Caries Ethics Committees, Research Fallopian Tubes Females Insemination Males Menstruation Ovarian Stimulation Pharmaceutical Preparations Semen Sperm Sterility, Reproductive Uterus Woman

Compiling Comprehensive Ovarian Cancer Datasets

As our purpose was to compile a database of broader generalizations and larger sample size, we adopted less stringent eligibility criteria [28 (link)]. Three private, one in-house and 44 publicly available microarray ovarian cancer datasets from Gene Omnibus (GEO; http://www.ncbi.nlm.nih.gov/gds), ArrayExpress (http://www.ebi.ac.uk/arrayexpress/), Expression Project for Oncology (ExpO; http://www.intgen.org/), and The Cancel Genome Atlas (TCGA; http://cancergenome.nih.gov/) were downloaded by Jan 2015 (Figure 1; Suppl. Table 1). Only datasets obtained using Affymetrix Microarrays HG-U133A (16.38%), HG-U133A2 (2.88%), HG-HT-U133A (17.25%), HG-U133-Plus2 (49.2%), and human gene 1.0 ST (14.29%) were used. These datasets are inclusive of primary and metastatic ovarian cancers, fallopian tube carcinoma, peritoneal carcinoma, ovarian cancer stroma, and normal ovarian surface epithelium, fallopian tube, and stroma tissues (Figure 2). No limit was imposed on the race, pre-treatment history or medical condition, stage, grade, or histology of the disease.

Tan T.Z., Yang H., Ye J., Low J., Choolani M., Tan D.S., Thiery J.P, & Huang R.Y. (2015). CSIOVDB: a microarray gene expression database of epithelial ovarian cancer subtype. Oncotarget, 6(41), 43843-43852.

Full text: Click here

Publication 2015

Cancer of the Fallopian Tube Carcinoma Eligibility Determination Epithelium Fallopian Tubes Generalization, Psychological Genes Genome Inclusion Bodies Microarray Analysis Neoplasms Ovarian Cancer Ovary Peritoneum Tissues

Generation of mt-Keima Transgenic Mice

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2015

Animals Animals, Transgenic Biopsy Cells Childbirth Cloning Vectors DNA, Complementary Embryo Fallopian Tubes Integrase Mice, Laboratory Mice, Transgenic Mothers Oligonucleotide Primers Recombination, Genetic RNA, Messenger Stem Cells Tail Zygote

Most recents protocols related to «Fallopian Tubes»

Fallopian Tube Luminal Fluid Collection

The sample collection process in the current study was approved by the Institute Review Board of Tongji Hospital (No. TJ-IRB20210838). Fallopian tubes were obtained from 23 premenopausal women (34–42 years old) undergoing hysterectomy. Luminal fluids were collected immediately after the uterus and the Fallopian tubes were resected, by flushing the lumen of the Fallopian tubes with 50 ml sterile PBS, as previously described (Bathala et al., 2018 ).

Li Y., Liu C., Guo N., Cai L., Wang M., Zhu L., Li F., Jin L, & Sui C. (2023). Extracellular vesicles from human Fallopian tubal fluid benefit embryo development in vitro. Human Reproduction Open, 2023(2), hoad006.

Publication 2023

Fallopian Tubes Hysterectomy Phenobarbital Specimen Collection Sterility, Reproductive Uterus Woman

Murine Embryo Isolation via Hormonal Induction

The animal experiment was approved by the ethical committee of Tongji Hospital (TJ-202111004). Eighty female (6–8 weeks of age) and ten male (8–10 weeks of age) Institute of Cancer Research (ICR) mice were used in the study. Female mice were injected i.p. with 10 IU pregnant mare’s serum gonadotrophin, followed by 10 IU hCG 48 h later. After the injection of hCG, two female mice were mated with one male mouse, for one night. Thirty-six hours after the hCG injection, the pregnant mice were identified and killed by cervical dislocation, and their Fallopian tubes were removed for two-cell embryo collection.

Publication 2023

Cells Embryo Fallopian Tubes Females Human Chorionic Gonadotropin Joint Dislocations Males Malignant Neoplasms Mice, House Neck Pregnant Mare Serum Gonadotropins

Profiling Fallopian Tube-Derived oEVs miRNAs

A total of eight oEVs samples (isolated from the Fallopian tubes of eight women) were used for total RNA extraction, using the exoRNeasy Maxi Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The quality of the RNA samples was examined using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA) and standard denaturing agarose gel electrophoresis. Small RNA library preparation was performed using TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, CA, USA). The quality-ensured RNA-seq libraries were then sequenced using Illumina Hiseq2000/2500. Identification of known miRNAs (mapped to the miRbase database) and read counting were processed using ACGT101-miR (LC Sciences, Houston, TX, USA). A modified normalization was used to correct copy number among different samples, as described previously (Li et al., 2016 ), and a miRNA was considered present when the normalized read count was >0 in all the samples. A heatmap was constructed using the normalized read counts of the known miRNAs in each oEV sample, using R (R version 4.0.3) with a heatmap via a custom written R script.

Publication 2023

DNA Library Electrophoresis, Agar Gel Fallopian Tubes MicroRNAs RNA-Seq Woman

Tissue-specific expression regulation in PTB

The Genotype-Tissue Expression (GTEx) database cataloged a large number of tissue-specific genotypes and shared regulatory expression quantitative trait loci (eQTL) variants. We used GTEx to identify the best expressed gene in the female reproductive tissues (bladder, cervix–ectocervix, endocerivx, fallopian tubes, ovaries, uterus, and vagina) that could impact PTB status at protein level [41 (link)]. After investigating the concerned genes/proteins with identified tissue-specific regulatory expression in female reproductive tissues, the Protein Data Bank (PDB) structural coverage details from previous reported studies were investigated from PDB [42 (link)].

Azmi M.B., Khan W., Azim M.K., Nisar M.I, & Jehan F. (2023). Identification of potential therapeutic intervening targets by in-silico analysis of nsSNPs in preterm birth-related genes. PLOS ONE, 18(3), e0280305.

Full text: Click here

Publication 2023

Cervix Uteri Ectocervix Fallopian Tubes Females Gene Expression Regulation Genes Genotype Ovary Polypyrimidine Tract-Binding Protein Proteins Quantitative Trait Loci Reproduction Tissues Tissue Specificity Urinary Bladder Uterus Vagina

Isolation of Mesenchymal Stem Cells from Fallopian Tubes

MSCs were isolated from healthy fallopian tubes (hFTs) collected in DMEM/Hams F-12 medium (Invitrogen, Carlsbad, CA) and kept at 4 °C for processing within 24 h as described previously [15] (link). Briefly, hFTs were opened, washed twice in PBS (Gibco, Invitrogen, Carlsbad, CA) and incubated at 37 °C for 30 min in a 50 mL Falcon tube containing 5 mL of pure TrypLE™ Express (Invitrogen, Carlsbad, CA) with shaking. The supernatant was removed, washed once with 7 mL of advanced DMEM/Hams F12 in a 15 mL Falcon tube and centrifuged at 1000 rpm for 5 min at room temperature. The cells were then plated in advanced DMEM/Hams F12 (12 mL) supplemented with penicillin/streptomycin (100 µg/mL) in 75 cm³ polystyrene culture flasks and incubated at 37 °C in a humidified atmosphere containing 5.0% CO₂ (Figure 1).

Abu-Lubad M.A., Al-Zereini W, & Al-Zeer M.A. (2023). Deregulation of the cyclin-dependent kinase inhibitor p27 as a putative candidate for transformation in Chlamydia trachomatis infected mesenchymal stem cells. AIMS Microbiology, 9(1), 131-150.

Full text: Click here

Publication 2023

ATF7IP protein, human Atmosphere Cells Fallopian Tubes Penicillins Polystyrenes Streptomycin

Top products related to «Fallopian Tubes»

Hyaluronidase by Merck Group

Sourced in United States, Germany, Switzerland, United Kingdom, Japan, China, Italy, France, Macao, Israel, Australia, Sao Tome and Principe, Canada, Spain, Netherlands, Czechia

Hyaluronidase is an enzyme used in laboratory settings. It functions by breaking down hyaluronic acid, a component of the extracellular matrix.

M2 medium by Merck Group

Sourced in United States, Germany, United Kingdom, France, Japan

M2 medium is a cell culture media formulation designed for the maintenance and cultivation of mouse embryos. It provides the necessary nutrients and growth factors to support the development and growth of mouse embryos in an in vitro environment.

Human chorionic gonadotropin (hcg) by Merck Group

Sourced in United States, Switzerland, Germany, China, United Kingdom, Canada, Israel, Macao, Sao Tome and Principe, Japan

The HCG (Human Chorionic Gonadotropin) is a laboratory equipment product by Merck Group. It is a hormone typically used in various diagnostic and research applications. The core function of HCG is to detect and measure the levels of this hormone in biological samples.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

M16 medium by Merck Group

Sourced in United States

M16 medium is a culture medium used for the isolation and cultivation of microorganisms. It provides the necessary nutrients and growth factors for the growth and maintenance of microbial cultures in a laboratory setting.

Ksom medium by Merck Group

Sourced in United States, United Kingdom, Germany

KSOM medium is a synthetic culture medium designed for the in vitro culture of mammalian embryos. It provides a defined and optimized environment for the growth and development of early-stage embryos.

Bovine serum albumin (bsa) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Japan, France, Sao Tome and Principe, Canada, Macao, Spain, Switzerland, Australia, India, Israel, Belgium, Poland, Sweden, Denmark, Ireland, Hungary, Netherlands, Czechia, Brazil, Austria, Singapore, Portugal, Panama, Chile, Senegal, Morocco, Slovenia, New Zealand, Finland, Thailand, Uruguay, Argentina, Saudi Arabia, Romania, Greece, Mexico

Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Ovcar3 by American Type Culture Collection

Sourced in United States, China, United Kingdom, Germany

OVCAR3 is a cell line derived from a human ovarian adenocarcinoma. It is commonly used in research for the study of ovarian cancer.

Trizol reagent by Thermo Fisher Scientific

Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand

TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.

What are the main functions of the Fallopian Tubes?

The Fallopian Tubes play a crucial role in human reproduction. They serve as the conduits for the egg to travel from the ovary to the uterus, and also provide the site for fertilization where the egg and sperm meet. The tubes have specialized ciliated cells that help propel the egg along the journey, and their muscular walls assist in the transport of the fertilized egg to the uterus for implantation.

What are some common medical conditions that can affect the Fallopian Tubes?

Some common conditions that can impact the Fallopian Tubes include tubal pregnancy, where the fertilized egg implants in the tube instead of the uterus, as well as pelvic inflammatory disease, which can lead to scarring and blockages in the tubes. Tubal ligation, a form of permanent birth control, involves surgically tying or sealing the Fallopian Tubes to prevent pregnancy.

How can PubCompare.ai help with Fallopian Tube research?

PubCompare.ai's AI-powered platform can assist researchers studying the Fallopian Tubes in several ways. The platform allows you to screen protocol literature more efficiently, leveraging AI to pinpoint critical insights. This can help you identify the most effective protocols related to Fallopian Tubes for your specific research goals. The platform's analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your studies.

Are there different types or variations of Fallopian Tubes?

While the basic structure of the Fallopian Tubes is generally consistent, there can be some natural variations. For example, the length of the tubes can vary, ranging from around 10 to 15 centimeters. The diameter of the tubes may also differ slightly from person to person. Additionally, the number of coils or twists in the tubes can vary, which can impact the path the egg takes during ovulation and fertilization.

What are some practical considerations when working with Fallopian Tube samples or models?

When conducting research involving Fallopian Tube samples or models, it's important to consider factors like tissue handling, preservation, and cultural differences. Proper sample collection and storage procedures are crucial to maintain the integrity of the specimens. Additionally, researchers should be mindful of any cultural or religious sensitivities that may surround the use of reproductive tissues. PubCompare.ai can help you navigate these practical considerations by providing access to the most relevant and effective protocols from the literature.

More about "Fallopian Tubes"

Fallopian Tubes: These intricate anatomical structures are essential for human reproduction, serving as the hidden pathways that transport the egg from the ovary to the uterus.
These remarkable features play a crucial role in fertility and deserve further exploration.
Discover the secrets of the Fallopian Tubes, an area of great scientific interest.
These tubular structures are lined with ciliated epithelial cells that help guide the egg on its journey.
Hyaluronidase, an enzyme found in the Fallopian Tubes, aids in the fertilization process by breaking down the protective layers surrounding the egg.
Optimizing research protocols is key to unlocking the full potential of Fallopian Tube studies.
PubCompare.ai, an AI-powered platform, offers a seamless way to identify the most effective products and procedures for your research needs.
Explore a vast database of literature, pre-prints, and patents to locate the best protocols, effortlessly comparing and contrasting different approaches.
Enhance your workflow with the power of AI.
PubCompare.ai can help you uncover the true secrets of the Fallopian Tubes, from the role of M2 medium and HCG in supporting egg development, to the importance of FBS and M16 medium in cell culture, to the use of Bovine serum albumin and Penicillin/streptomycin in maintaining a healthy environment.
Delve deeper into the world of Fallopian Tubes and their connection to fertility.
Discover how OVCAR3 cell lines and TRIzol reagent can be utilized in your research.
Unleash the power of AI to improve your research outcomes and uncover the extraordinary potential of these remarkable anatomical features.