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Foreskin
The foreskin is the double-layered fold of skin that covers the glans of the penis.
It protects the glans and facilitates sexual functions.
Foreskin studies investigate the anatomy, development, and clinical aspects of this structure, which can be involved in various medical conditions and procedures.
Researchers can utilize PubCompare.ai's innovative platform to streamline their foreskin-related research, easily accessing optimized protocols from the literature, preprints, and patents, and leveraging AI-powered comparisons to identify the best approaches and products for their studies.
It protects the glans and facilitates sexual functions.
Foreskin studies investigate the anatomy, development, and clinical aspects of this structure, which can be involved in various medical conditions and procedures.
Researchers can utilize PubCompare.ai's innovative platform to streamline their foreskin-related research, easily accessing optimized protocols from the literature, preprints, and patents, and leveraging AI-powered comparisons to identify the best approaches and products for their studies.
Most cited protocols related to «Foreskin»
Biological Assay
Cell Encapsulation
Cell Nucleus
Cells
Cell Survival
Cytotoxin
ethidium homodimer
Fetal Bovine Serum
Fibroblasts
fluorexon
Foreskin
Fungus, Filamentous
Homo sapiens
Hydrogels
Infant, Newborn
Microscopy
Microscopy, Confocal
Phosphates
poly(ethylene glycol)diacrylate
Polymerization
Rubber
Saline Solution
Staining
Tissue, Membrane
Parasites were grown in hTERT-BJ1 (clontech) cells in supplemented Dulbecco's modified Eagle's medium [67] ). Parasite cloning and plaque assays were performed in human foreskin fibroblasts (HFF). For the selection of stable transgenic lines, drugs were added as follow: 1 µM pyrimethamine added one day after transfection for one week, 20 µM chloramphenicol added the day of transfection for three weeks, 5 µM FUDR added two days after transfection for one week. To repress the regulated promoter, parasites were grown in the presence of 0.5 µM anhydrotetracycline (ATc).
Thalassiosira pseudonana (Hustedt) Hasle et Heimdal CCMP1335 was grown in an artificial seawater medium (EASW) according to the North East Pacific Culture Collection protocol (http://www3.botany.ubc.ca/cccm/NEPCC/esaw.html ) at 18°C under constant light. Where indicated, NaNO3 was omitted from the medium (nitrogen-free medium) or replaced by 0.55 mM NH4Cl (ammonium medium).
Thalassiosira pseudonana (Hustedt) Hasle et Heimdal CCMP1335 was grown in an artificial seawater medium (EASW) according to the North East Pacific Culture Collection protocol (
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Ammonium
anhydrotetracycline
Animals, Transgenic
Biological Assay
Cells
Chloramphenicol
Fibroblasts
Floxuridine
Foreskin
Homo sapiens
Light
Nitrogen
Parasites
Pharmaceutical Preparations
Pyrimethamine
Senile Plaques
Transfection
The T. gondii strains used in this study were maintained by growth in human foreskin fibroblasts (HFF) cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 10 µg/ml gentamicin, and 10 mM glutamine (Thermo, Fisher Scientific, Waltham, MA). Wild-type RH and GT1 and the parasite mutant lines RH Δku80 and RH Δku80Δrop18 were described previously (9 (link)). Transgenic parasites were obtained by electroporation of constructs into cells and selection with 3 µM pyrimethamine (Sigma-Aldrich, St. Louis, MO) or 10 µM FUDR (Sigma-Aldrich, St. Louis, MO).
Animals, Transgenic
Cells
Culture Media
Eagle
Electroporation
Fetal Bovine Serum
Fibroblasts
Foreskin
Gentamicin
Glutamine
Homo sapiens
Parasites
Pyrimethamine
Strains
Bos taurus
Calcium
Cells
Epidermal growth factor
Fetal Bovine Serum
Foreskin
Homo sapiens
Keratinocyte
Epidermal keratinocytes were isolated from human foreskin as previously described (Halbert et al., 1992 (link)). The cells were propagated in medium 154 supplemented with human keratinocyte growth supplement, 1,000× gentamycin/amphotericin B solution (Invitrogen), and 0.07 or 0.2 mM CaCl2.
Keratinocytes were transduced with retroviral supernatants produced from Phoenix cells (provided by G. Nolan, Stanford University, Stanford, CA) as previously described (Getsios et al., 2004 (link)). For differentiation of submerged cultures, cells were grown to confluence and switched to E-medium containing 1.8 mM Ca2+ for 1–6 d (Meyers and Laimins, 1994 (link)). For raft cultures, transduced cells were expanded and grown at an air–medium interface according to published protocols (Meyers and Laimins, 1994 (link)). Organotypic cultures were grown for 3–10 d, at which time they were lysed for RNA/protein analysis, embedded in optimal cutting temperature compound for frozen sections, fixed in 10% neutral-buffered formalin, and embedded in paraffin for histology or fixed in 2% paraformaldehyde/2% glutaraldehyde in cacodylate buffer for EM analysis. For some experiments, cultures were treated with 2–5 µg/ml ETA, DMSO (Thermo Fisher Scientific), 10 µM PKI166 (Novartis), 5 µM U0126 (Cell Signaling Technology), or 10 µM SB203580 (EMD).
Keratinocytes were transduced with retroviral supernatants produced from Phoenix cells (provided by G. Nolan, Stanford University, Stanford, CA) as previously described (Getsios et al., 2004 (link)). For differentiation of submerged cultures, cells were grown to confluence and switched to E-medium containing 1.8 mM Ca2+ for 1–6 d (Meyers and Laimins, 1994 (link)). For raft cultures, transduced cells were expanded and grown at an air–medium interface according to published protocols (Meyers and Laimins, 1994 (link)). Organotypic cultures were grown for 3–10 d, at which time they were lysed for RNA/protein analysis, embedded in optimal cutting temperature compound for frozen sections, fixed in 10% neutral-buffered formalin, and embedded in paraffin for histology or fixed in 2% paraformaldehyde/2% glutaraldehyde in cacodylate buffer for EM analysis. For some experiments, cultures were treated with 2–5 µg/ml ETA, DMSO (Thermo Fisher Scientific), 10 µM PKI166 (Novartis), 5 µM U0126 (Cell Signaling Technology), or 10 µM SB203580 (EMD).
Amphotericin
Amphotericin B
Buffers
Cacodylate
Cells
Dietary Supplements
Epidermis
Foreskin
Formalin
Frozen Sections
Gentamicin
gentamicin B
Glutaral
Homo sapiens
Keratinocyte
Microphysiological Systems
Paraffin Embedding
paraform
PKI 166
Proteins
Retroviridae
SB 203580
Sulfoxide, Dimethyl
U 0126
Most recents protocols related to «Foreskin»
Example 1
The pluripotent stem cell line H9 was obtained from NIH line WA 09, supplied by WiCell (Madison, Wis.) and was maintained in an undifferentiated state by passaging on irradiated human foreskin fibroblasts (line HS27, ATCC, Manassas, Va.) and gelatin coated plates. To differentiate the pluripotent stem cells towards a mesodermal and then mesenchymal lineage, the colonies of the pluripotent stem cells were mechanically dissected into small pieces under microscopic guidance and then transferred to tissue culture-treated 6-well plates (Corning). The cells at this stage were considered passage 0 (P0). The cells were cultured in DMEM/F12 supplemented with non-essential amino acids and 10% fetal bovine serum (FBS, Invitrogen-Gibco, Grand Island, N.Y.). When the culture approached confluency, cells were trypsinized and transferred to a new tissue culture flask.
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Amino Acids, Essential
Cells
Fibroblasts
Foreskin
Gelatins
Homo sapiens
Mesenchyma
Mesoderm
Microscopy
Pluripotent Stem Cells
Tissues
Human foreskin fibroblast Hs68 cells (ATCC CRL-1635, BCRC60038) were purchased from Bioresource Collection and Research Center, Hsinchu, Taiwan. Human keratinocyte HaCaT cells (CLS 300,493) were purchased from Cell Line Service GmbH (Eppelheim, Germany). HaCaT was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2 mM sodium pyruvate, and Hs68 was cultured in DMEM. These media contain 10% fetal bovine serum and 100 units/mL antibiotics. The cells were grown at 37 °C in a 5% CO2 incubator. All cell culture media and reagents were of reagent grade or cell-culture grade purchased from Sigma-Aldrich (St. Louis, MO, USA) or Gibco (Thermo Fisher Scientific, Inc., Carlsbad, CA, USA).
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Antibiotics
Cell Culture Techniques
Cell Lines
Cells
Culture Media
Eagle
Fetal Bovine Serum
Fibroblasts
Foreskin
HaCaT Cells
Homo sapiens
Keratinocyte
Pyruvate
Sodium
Psoriatic skin biopsies were taken from untreated adult male and female patients presenting at the Department of Dermatology of the Geneva University Hospitals in Switzerland (age 38–50). Healthy skin biopsies were taken from adult female patients presenting at the Department of Plastic and Reconstructive Surgery and Division of Clinical Pathology of the Geneva University Hospitals in Switzerland (age 34–58). The foreskin biopsies for the isolation of primary keratinocytes were taken from male children (age 1–16) undergoing surgery at the Polish-American Children’s Hospital, Krakow, Poland. This study was conducted according to the Declaration of Helsinki and approved by the local ethical committee of the University Hospitals of Geneva, Switzerland, and the Jagiellonian University according to Polish law (No. 1072.6120.9.2017). Written informed consent was obtained for each individual.
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Adult
Biopsy
Child
Foreskin
isolation
Keratinocyte
Males
Operative Surgical Procedures
Patients
Reconstructive Surgical Procedures
Skin
Woman
Human primary keratinocytes (HEK) were derived from prepuce tissue discarded after surgery in children 3–14 years of age. The study was approved by the ethics committee of the Affiliated Hospital of Guizhou Medical University. All subjects’ parents or guardians provided written informed consent. Purified HEK was obtained through a two-step enzyme digestion method and was grown in EpiLife® medium (MEPI500CA, Invitrogen Cascade Biologics, USA) with human keratinocyte growth supplement (HKGS, S0015, Invitrogen Cascade Biologics, USA), 2 mM L-glutamine (1051024, Gibco, USA), and a 1% penicillin and streptomycin solution (2114092, Biological Industries, Israel). HEK in the second passage was used in this study. Human immortalized keratinocytes (HaCaT) were purchased from Kunming Cell Bank of Type Culture Collection, Chinese Academy of Science (KCB200442YJ, Kunming, China). HaCaT was cultured in Dulbecco’s modified Eagle’s medium (DMEM, 0030034DJ, Gibco, USA) containing 10% fetal bovine serum (FBS, FBSSA500-S, AUS GeneX) and a 1% penicillin and streptomycin solution and cultured in an incubator with 5% CO2 at 37 °C. HaCaT in passages 10–25 was used in this study.
Human primary melanocytes (MC) were obtained from human children’s foreskin with a two-step enzyme digestion method, and MC was immediately established in Medium 254(M254500, Gibco, USA) supplemented with a human melanocyte growth supplement (S0165, Gibco, USA), 2 mM L-glutamine, and 1% penicillin and streptomycin solution.
Human primary melanocytes (MC) were obtained from human children’s foreskin with a two-step enzyme digestion method, and MC was immediately established in Medium 254(M254500, Gibco, USA) supplemented with a human melanocyte growth supplement (S0165, Gibco, USA), 2 mM L-glutamine, and 1% penicillin and streptomycin solution.
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Biological Factors
Biopharmaceuticals
Cell Culture Techniques
Child
Chinese
Dietary Supplements
Digestion
Eagle
Enzymes
Ethics Committees, Clinical
Foreskin
Glutamine
Homo sapiens
Keratinocyte
Legal Guardians
Melanocyte
Operative Surgical Procedures
Parent
Penicillins
Pepsin A
Streptomycin
Tissues
T. gondii tachyzoites ME49 (clone B7–21), ME49-GFP-Luc1 and Pru-Δhxgprt-tdTomato (Pru-tdTomato)2 were propagated in confluent human foreskin fibroblasts (HFF1) in DMEM medium containing high glucose supplemented with 100 U ml−1 penicillin, 100 mg ml−1 streptomycin and 2 % fetal bovine serum.
Clone Cells
Fetal Bovine Serum
Fibroblasts
Foreskin
Glucose
Homo sapiens
Penicillins
Streptomycin
tdTomato
Top products related to «Foreskin»
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
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FBS, or Fetal Bovine Serum, is a commonly used cell culture supplement. It is derived from the blood of bovine fetuses and provides essential growth factors, hormones, and other nutrients to support the growth and proliferation of a wide range of cell types in vitro.
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EpiLife medium is a serum-free, low-calcium cell culture medium designed to support the growth and maintenance of human epidermal keratinocytes. It provides a defined environment for the culture of these cells.
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DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium formulated to support the growth and maintenance of various cell lines. It provides a balanced salt solution and essential nutrients required for cell proliferation. DMEM is suitable for use in a wide range of cell culture applications.
More about "Foreskin"
The foreskin, also known as the prepuce, is a double-layered fold of skin that covers the glans (head) of the penis.
This anatomical structure serves to protect the sensitive glans and facilitates various sexual functions.
Researchers studying the foreskin can delve into its anatomy, development, and clinical aspects, which can be involved in various medical conditions and procedures.
When conducting foreskin-related research, scientists may utilize specialized cell culture media and supplements to support the growth and maintenance of foreskin-derived cells.
These can include Dulbecco's Modified Eagle Medium (DMEM), which provides essential nutrients, and supplements like Penicillin/Streptomycin to prevent bacterial contamination.
L-glutamine and GlutaMAX may also be added to the culture medium to support cell metabolism.
Additionally, EpiLife medium is a specialized formulation designed for the culture of epidermal cells, which can be particularly useful for studies involving the foreskin's epithelial layer.
Researchers can streamline their foreskin studies by utilizing PubCompare.ai's innovative platform.
This tool allows them to easily access optimized research protocols from the scientific literature, preprints, and patents, and leverage AI-powered comparisons to identify the best approaches and products for their specific investigations.
By leveraging this platform, scientists can enhance the efficiency and effectiveness of their foreskin-related research projects.
This anatomical structure serves to protect the sensitive glans and facilitates various sexual functions.
Researchers studying the foreskin can delve into its anatomy, development, and clinical aspects, which can be involved in various medical conditions and procedures.
When conducting foreskin-related research, scientists may utilize specialized cell culture media and supplements to support the growth and maintenance of foreskin-derived cells.
These can include Dulbecco's Modified Eagle Medium (DMEM), which provides essential nutrients, and supplements like Penicillin/Streptomycin to prevent bacterial contamination.
L-glutamine and GlutaMAX may also be added to the culture medium to support cell metabolism.
Additionally, EpiLife medium is a specialized formulation designed for the culture of epidermal cells, which can be particularly useful for studies involving the foreskin's epithelial layer.
Researchers can streamline their foreskin studies by utilizing PubCompare.ai's innovative platform.
This tool allows them to easily access optimized research protocols from the scientific literature, preprints, and patents, and leverage AI-powered comparisons to identify the best approaches and products for their specific investigations.
By leveraging this platform, scientists can enhance the efficiency and effectiveness of their foreskin-related research projects.