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Kidney Cortex
The kidney cortex is the outer portion of the kidney, composed of numerous nephrons and blood vessels.
It is responsible for the initial filtration of blood and the reabsorption of water, nutrients, and other substances.
The kidney cortex plays a crucial role in maintaining fluid and electrolyte balance, as well as regulating blood pressure and the production of hormones such as renin and erythropoietin.
Studying the kidney cortex is important for understanding normal kidney function and the pathogenesis of kidney diseases, such as glomerulonephritis, diabetic nephropathy, and chronic kidney disease.
Researchers can utilize PubCompare.ai, an AI-driven platform, to optimisze their kidney cortex research by easily locating the best protocols from literature, preprints, and patents using AI-driven comparisons to enhance reproducibiltiy and accuracy, streamlining the research process and finding the most effective methods.
It is responsible for the initial filtration of blood and the reabsorption of water, nutrients, and other substances.
The kidney cortex plays a crucial role in maintaining fluid and electrolyte balance, as well as regulating blood pressure and the production of hormones such as renin and erythropoietin.
Studying the kidney cortex is important for understanding normal kidney function and the pathogenesis of kidney diseases, such as glomerulonephritis, diabetic nephropathy, and chronic kidney disease.
Researchers can utilize PubCompare.ai, an AI-driven platform, to optimisze their kidney cortex research by easily locating the best protocols from literature, preprints, and patents using AI-driven comparisons to enhance reproducibiltiy and accuracy, streamlining the research process and finding the most effective methods.
Most cited protocols related to «Kidney Cortex»
Cortex, Cerebral
Kidney Cortex
Below we give a brief summary of the datasets used in the construction of the reference databases (see also Table 1 ).
Main Illumina 450k DNAm datasets used. We list the main datasets used in this study, the cell-types/tissue profiled, whether the data was used for reference database construction (if yes, we specify which cell-types were used), whether the data was used for validation/evaluation purposes (if yes, we specify which cell-types were used) and the reference/citation
| Dataset Name | Tissue/cell-types | Use in Reference DNAm Database | Testing/Evaluation | Reference |
|---|---|---|---|---|
| Reinius et al. | WB, PBMC, NK, B, CD4T, CD8T, Monoc, Neutro, Eosino. (n = 6 of each) | NK, B, CD4T, CD8T, Monoc., Neutro., Eosino. | WB & PBMC | [24 (link)] |
| Liu et al. | WB (n = 335 controls, n = 354 rheumathoid arthritis cases) | No | Average Flow Cytometry estimates for cases and controls | [2 (link)] |
| Koestler et al. | WB (n = 18) | No | 12 Reconstructed WB mixtures + 6 WB samples with Flow Cytometry estimates | [20 (link)] |
| Zilbauer et al. | PBMC, CD4T, CD8T, NK, B, Monoc, Neutro. (n = 6 of each) | No | In-silico mixtures of purified blood cell subtypes | [27 (link)] |
| ENCODE | Various | HMEC, HRCE, IMR90, Liver | No | [22 (link)] |
| Slieker et al. | Various | Pancreas | Liver | [26 (link)] |
| SCM2 | Various | No | HRCE, Pancreas, IMR90 | [29 (link)] |
| Lowe et al. | Various | No | HMEC | [28 (link), 35 (link)] |
| Teschendorff et al. | WB (n = 152) | No | Smoking associated DMCs | [31 (link)] |
Abbreviations: DNAm = DNA methylation, WB = whole blood, PBMC = peripheral blood mononuclear cells, HMEC = human mammary epithelial cells, HRCE = human renal cortical epithelia, IMR90 (fetal lung fibroblast), SCM2 = Stem-Cell-Matrix Compendium-2, DMCs = differentially methylated CpGs, NK = natural killer cells, B = B-cell, Monoc = Monocytes, Neutro. = Neutrophils, Eosino = Eosinophils, CD4T = CD4+ T-cells, CD8T = CD8+ T-cells
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Arthritis
B-Lymphocytes
BLOOD
Blood Cells
Breast
CD4 Positive T Lymphocytes
CD8-Positive T-Lymphocytes
cytidylyl-3'-5'-guanosine
DNA Methylation
Eosinophil
Epithelial Cells
Epithelium
Fetus
Fibroblasts
Flow Cytometry
Homo sapiens
Kidney Cortex
Lung
Monocytes
Natural Killer Cells
Neutrophil
Pancreas
PBMC Peripheral Blood Mononuclear Cells
Stem Cells
Tissues
Cortex, Cerebral
Kidney Cortex
Magnetic Resonance Imaging
Microtubule-Associated Proteins
Aldehydes
Autopsy
Biopsy
Body Temperature Changes
Fixatives
Formaldehyde
Glutaral
Homo sapiens
Kidney Cortex
Perfusion
Phosphates
Saline Solution
Submersion
Tissues
Cortex, Cerebral
Eye
Kidney Cortex
Movement
Young Adult
Most recents protocols related to «Kidney Cortex»
Example 70
Heat lauric acid, stearic acid, myristic acid, oleic acid, squalene oil, rosehip oil, lemongrass oil, olive oil, and/or jojoba oil to about 75° C. Then dissolve NaOH in water (or an initial aliquot of water) and heat the solution to about 70° C. To the NaOH solution add glycerin and silk, then add the blend of lauric acid, stearic acid, myristic acid, oleic acid, squalene oil, rosehip oil, lemongrass oil, olive oil, and/or jojoba oil. Allow the mixture to cool. Where applicable, reheat the mixture and add rosehip oil, squalene oil, and/or 2M HCl. Then, where applicable, add an ending aliquot of water and/or olive oil. Finally, add aspen bark and/or sodium anisate to yield the combination cleanser.
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Glycerin
jojoba wax
Kidney Cortex
lauric acid
Myristic Acid
Oil, Olive
Oleic Acid
Rosehips
Silk
Sodium
Squalene
stearic acid
west indian lemongrass oil
Example 96
In an embodiment, an exemplary foot balm formulation that may provide a footbalm product may include:
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beeswax
Butter
Eucalyptus Oil
Foot
Glycerin
glyceryl caprylate
Kidney Cortex
Microbicides
Oil, Coconut
Oil, Sunflower
Oils, Volatile
Pharmaceutical Preservatives
rosemary oil
Silk
Sodium
Sodium Phytate
Tea Tree Oil
Vitamin E
west indian lemongrass oil
Example 61
Dissolve hyaluronic acid in water to prepare a solution and then add silk slowly. Refrigerate the solution. Heat a combination of lauric acid, jojoba oil, myristic acid, and/or stearic acid to about 75° C. Then, heat the hyaluronic acid/silk solution to about 55° C. Add the acid/jojoba oil combination to the hyaluronic acid/silk solution and mix. Allow the solution to cool and then add aspen bark and sodium anisate to provide the cleanser.
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Acids
Hyaluronic acid
jojoba wax
Kidney Cortex
lauric acid
Myristic Acid
Silk
Sodium
stearic acid
Example 95
In an embodiment, a foot balm formulation that may provide a foot balm product may include:
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beeswax
Butter
Eucalyptus Oil
Foot
Glycerin
glyceryl caprylate
Kidney Cortex
Microbicides
Oil, Coconut
Oil, Sunflower
Oils, Volatile
Pharmaceutical Preservatives
rosemary oil
Silk
Sodium
Sodium Phytate
Tea Tree Oil
Vitamin E
west indian lemongrass oil
Total RNA was extracted from the renal cortex and cell using the corresponding detection kits in accordance with the manufacturer’s instructions. cDNA was synthesized using a reverse transcription kit. Real-time quantitative PCR was performed using SYBR Green PCR Master Mix on a Roche Light Cycler 480 system. PCR primer sequences are shown in Table 1 (Chen et al., 2013 (link); Li et al., 2022 (link)). The relative mRNA levels were calculated using the 2−ΔΔCT formula.
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Cells
DNA, Complementary
Kidney Cortex
Methyl Green
Oligonucleotide Primers
Real-Time Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.
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More about "Kidney Cortex"
The kidney cortex, also known as the renal cortex, is the outermost layer of the kidney.
It is composed of numerous nephrons and blood vessels, and is responsible for the initial filtration of blood and the reabsorption of water, nutrients, and other substances.
This crucial structure plays a vital role in maintaining fluid and electrolyte balance, regulating blood pressure, and producing important hormones such as renin and erythropoietin.
Studying the kidney cortex is essential for understanding normal kidney function and the pathogenesis of various kidney diseases, including glomerulonephritis, diabetic nephropathy, and chronic kidney disease.
Researchers can utilize cutting-edge technologies like the PubCompare.ai platform to streamline their kidney cortex research.
This AI-driven platform allows researchers to easily locate the best protocols from literature, preprints, and patents, using AI-driven comparisons to enhance reproducibility and accuracy.
In addition to the PubCompare.ai platform, researchers may also leverage common laboratory techniques and reagents to investigate the kidney cortex.
For example, TRIzol reagent can be used for RNA extraction, Neurobasal medium and Penicillin/streptomycin can be used for cell culture, and FBS, GlutaMAX, and B27 supplement can be used to supplement the culture media.
The RNeasy Mini Kit and TRIzol can be used for RNA purification, while DMEM and PVDF membranes may be utilized in various experimental procedures.
By combining the insights gained from the MeSH term description, the metadescription, and the incorporation of relevant laboratory techniques and reagents, researchers can optimize their kidney cortex studies, leading to a more efficient and effective research process.
It is composed of numerous nephrons and blood vessels, and is responsible for the initial filtration of blood and the reabsorption of water, nutrients, and other substances.
This crucial structure plays a vital role in maintaining fluid and electrolyte balance, regulating blood pressure, and producing important hormones such as renin and erythropoietin.
Studying the kidney cortex is essential for understanding normal kidney function and the pathogenesis of various kidney diseases, including glomerulonephritis, diabetic nephropathy, and chronic kidney disease.
Researchers can utilize cutting-edge technologies like the PubCompare.ai platform to streamline their kidney cortex research.
This AI-driven platform allows researchers to easily locate the best protocols from literature, preprints, and patents, using AI-driven comparisons to enhance reproducibility and accuracy.
In addition to the PubCompare.ai platform, researchers may also leverage common laboratory techniques and reagents to investigate the kidney cortex.
For example, TRIzol reagent can be used for RNA extraction, Neurobasal medium and Penicillin/streptomycin can be used for cell culture, and FBS, GlutaMAX, and B27 supplement can be used to supplement the culture media.
The RNeasy Mini Kit and TRIzol can be used for RNA purification, while DMEM and PVDF membranes may be utilized in various experimental procedures.
By combining the insights gained from the MeSH term description, the metadescription, and the incorporation of relevant laboratory techniques and reagents, researchers can optimize their kidney cortex studies, leading to a more efficient and effective research process.