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Lamina 1

Lamina 1 is a critical region of the spinal cord involved in the processing and transmission of sensory information.
It is the most superficial layer of the dorsal horn and plays a key role in pain perception, temperature sensation, and touch.
Researchers studying Lamina 1 physiology and function can utilize PubCompare.ai to identify the best experimental protocols and products from the literature, preprints, and patents.
This AI-driven platform enhances reproducibility and accuracy, helping advancse Lamina 1 research with ease.
PubCompare.ai provides a convinient way to find the right tools and methods to optimize Lamina 1 studies.

Most cited protocols related to «Lamina 1»

1
Quality Control for Single-Cell RNA-Seq
Nuclei were included for clustering analysis if they passed all of the following QC thresholds:
>30% cDNA longer than 400 base pairs
>500,000 reads aligned to exonic or intronic sequence
>40% of total reads aligned
>50% unique reads
TA nucleotide ratio > 0.7
After clustering (see below), clusters were identified as outliers if more than half of nuclei co-expressed markers of inhibitory (GAD1, GAD2) and excitatory (SLC17A7) neurons or were NeuN+ but did not express the pan-neuronal marker SNAP25. Median values of QC metrics listed above were calculated for each cluster and used to compute the median and inter-quartile range (IQR) of all cluster medians. Clusters were also identified as outliers if the cluster median QC metrics deviated by more than three times the IQRs from the median of all clusters. In total, 15,928 nuclei passed QC criteria and were split into three broad classes of cells (10,708 excitatory neurons, 4,297 inhibitory neurons, and 923 non-neuronal cells) based on NeuN staining and cell class marker gene expression
Clusters were identified as donor-specific if they included fewer nuclei sampled from donors than expected by chance. For each cluster, the expected proportion of nuclei from each donor was calculated based on the laminar composition of the cluster and laminar sampling of the donor. For example, if 30% of layer 3 nuclei were sampled from a donor, then a layer 3-enriched cluster should contain approximately 30% of nuclei from this donor. In contrast, if only layer 5 were sampled from a donor, then the expected sampling from this donor for a layer 1-enriched cluster was zero. If the difference between the observed and expected sampling was greater than 50% of the number of nuclei in the cluster, then the cluster was flagged as donor-specific and excluded. In total, 325 nuclei were assigned to donor-specific or outlier clusters that contained marginal quality nuclei and were excluded from further analysis. Three donor-specific clusters came from neurosurgical donors (n=95 nuclei) and were similar to other layer 5 types reported in our analysis, but had higher expression of activity-dependent genes.
To confirm exclusion, clusters automatically flagged as outliers or donor-specific were manually inspected for expression of broad cell class marker genes, mitochondrial genes related to quality, and known activity-dependent genes.
Hodge R.D., Bakken T.E., Miller J.A., Smith K.A., Barkan E.R., Graybuck L.T., Close J.L., Long B., Johansen N., Penn O., Yao Z., Eggermont J., Hollt T., Levi B.P., Shehata S.I., Aevermann B., Beller A., Bertagnolli D., Brouner K., Casper T., Cobbs C., Dalley R., Dee N., Ding S.L., Ellenbogen R.G., Fong O., Garren E., Goldy J., Gwinn R.P., Hirschstein D., Keene C.D., Keshk M., Ko A.L., Lathia K., Mahfouz A., Maltzer Z., McGraw M., Nguyen T.N., Nyhus J., Ojemann J.G., Oldre A., Parry S., Reynolds S., Rimorin C., Shapovalova N.V., Somasundaram S., Szafer A., Thomsen E.R., Tieu M., Quon G., Scheuermann R.H., Yuste R., Sunkin S.M., Lelieveldt B., Feng D., Ng L., Bernard A., Hawrylycz M., Phillips J.W., Tasic B., Zeng H., Jones A.R., Koch C, & Lein E.S. (2019). Conserved cell types with divergent features in human versus mouse cortex. Nature, 573(7772), 61-68.
Publication 2019
Cell Nucleus Cells DNA, Complementary Donors Exons Gene Expression Genes Genes, Mitochondrial glutamate decarboxylase 1 (brain, 67kDa), human Introns Lamina 1 Neuroglia Neurons Nucleotides Psychological Inhibition SNAP25 protein, human Tissue Donors
2
Quantifying NK1R Internalization in Lamina I Neurons

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Publication 2009
Animals Carisoprodol Endosomes Lamina 1 Microscopy, Fluorescence Nervousness Neurons Plant Roots Posterior Horn of Spinal Cord
3
Demineralization and Mechanical Testing of Dentin

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Publication 2010
Copper Dental Enamel Dentin Diamond Digital Radiography Homo sapiens Lamina 1 Matrix Metalloproteinases Minerals MMP2 protein, human Normal Saline phosphoric acid Sodium Azide Strains Third Molars Tooth
4
Ovarian Hormone Modulation of Itch Sensitivity
All experimental procedures were approved in accordance with the Guide for the Care and Use of Laboratory Animals (44 ) prepared by Okayama University (Okayama, Japan), by Kyoto Prefectural University of Medicine (Kyoto, Japan), by Toyama University (Toyama, Japan), and by the National Institute of Genetics (Shizuoka, Japan) and performed in accordance with the NIH guidelines on animal care. Adult wild-type Wistar rats and GRPR-mRFP transgenic rats were used in this study. To examine the effects of female sex steroid hormones, ovariectomized females were implanted with a blank capsule, a capsule containing physiological levels of 17β-estradiol, a capsule containing physiological levels of progesterone, or a capsule containing physiological levels of both hormones, for 1 to 2 mo. For itch behavioral analysis, rats received either saline, 3% histamine, or 10% CQ diphosphate salt diluted in saline via intradermal injection in the nape of the neck, cheek, or hind paw. Immediately after the injection, the rat was placed into the arena and videotaped from above for 60 min for scratching behavior. Mechanical sensitivity was assessed by the von Frey filament test, and thermal pain sensitivity was assessed by the Hargreaves test. For RT-PCR, enzyme-linked immunosorbent assay, Western blot, and the ChIP assay, the dorsal horns of the cervical spinal cords were collected and used for analysis. Complete methods are described in SI Appendix. Brain and spinal cord sections were used for immunofluorescence and immunoperoxidase histochemistry after perfusion with physiological saline followed by 4% paraformaldehyde in 0.1 M phosphate buffer. Antibody information is provided in SI Appendix, Fig. S2. In vivo extracellular single-unit recordings of superficial spinal dorsal horn (lamina I and II) neurons were performed in female rats. Statistical analyses were performed using SPSS Statistics version 27 (IBM). Graphs were made using GraphPad Prism 8 (GraphPad Software). Statistical analyses for each study are indicated in the figure legends. More detailed information on materials and methods is provided in SI Appendix.
Takanami K., Uta D., Matsuda K.I., Kawata M., Carstens E., Sakamoto T, & Sakamoto H. (2021). Estrogens influence female itch sensitivity via the spinal gastrin-releasing peptide receptor neurons. Proceedings of the National Academy of Sciences of the United States of America, 118(31), e2103536118.
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Publication 2021
Adult Animals Animals, Laboratory Brain Buffers Capsule Cheek Cytoskeletal Filaments Diphosphates Enzyme-Linked Immunosorbent Assay Estradiol Females Gonadal Steroid Hormones Histamine Histocytochemistry Hormones Hypersensitivity Immunofluorescence Immunoglobulins Immunoperoxidase Techniques Immunoprecipitation, Chromatin Intradermal Injection Lamina 1 Neck Neurons paraform Perfusion Pharmaceutical Preparations Phosphates physiology Posterior Horn of Spinal Cord prisma Progesterone Pruritus Rats, Transgenic Rats, Wistar Rattus norvegicus Reverse Transcriptase Polymerase Chain Reaction Saline Solution Sodium Chloride, Dietary Spinal Cord Western Blotting
5
Optimizing 3D Printed Tensile Sample Design
The dogbone samples for tensile tests were printed on PRUSA i3 MK2 3D printer (Prusa Research s.r.o., Praha, Czech Republic) by the FDM technology from PHB/PLA/plasticizer filaments with defined diameter, as mentioned above. AutoCAD (ver. 2018, Autodesk Inc., San Rafael, CA, USA) and Slic3r (software version 1.3.0, free software, developed by Alessandro Ranellucci) program were used for 3D virtual modeling and mathematical slicing. The dogbones were printed at 190 °C (195 °C during the first layers printing for better adhesion to the printing bed), at ambient conditions, without additional air cooling and bed heating. 3D printing was executed approximately six months after the filaments preparation.
The evolution of 3D printed dogbone models that were prepared for further tensile tests can be seen in Figure 2.
The Version I was created by simple import of fabricated model to Slic3r program and uploading to the printer. But, during the tensile test with dogbones of Version I, the delamination of perimeters occurred (illustrated by yellow color), and so the measured samples did not provide relevant results. To solve this problem, Version II was designed, where the perimeters of dogbone neck were elongated up to the upper part. The paddleboards were created from two parts. Unfortunately, during the tensile test, the defect at the point of paddleboard and neck connection occurred, because the printer created an inclusion in the paddleboard corner by releasing a large amount of melting. The inclusion acted as a disruption initiator and cracked untimely. The aim of Version III was to eliminate this problem, but the pressure of grips was not strong enough to hold the sample. The deformation then occurred in upper cross-sectional part and disagreed with real dogbone strength. The final Version (that could not be illustrated in Figure 2) was created by the combination of 10 laminas of Version I and IV, which alternate regularly (five from each Version). The delamination of the neck in its extending part was eliminated by the insertion of vertical infill (illustrated by red color) and the vertical fracture of samples was eliminated by the combination with horizontal infill.
Menčík P., Přikryl R., Stehnová I., Melčová V., Kontárová S., Figalla S., Alexy P, & Bočkaj J. (2018). Effect of Selected Commercial Plasticizers on Mechanical, Thermal, and Morphological Properties of Poly(3-hydroxybutyrate)/Poly(lactic acid)/Plasticizer Biodegradable Blends for Three-Dimensional (3D) Print. Materials, 11(10), 1893.
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Publication 2018
Biological Evolution Cytoskeletal Filaments Fracture, Bone Grasp Lamina 1 Neck Perimetry Plasticizers Pressure Vision

Most recents protocols related to «Lamina 1»

1
Isolation and Characterization of Adipocytes
Tissue samples of EAT and SAT were obtained in the course of CABG in the amount of 0.2–1 g as described before [21 (link)]. Briefly, the samples were placed in M199 medium preheated to 37 °C in advance and delivered to the laboratory within 15 min. Adipocytes were isolated enzymatically in sterile conditions (laminar box BAVp-01- “Laminar-s” −1.5, ZAO “Laminar systems”, Miass, Russia) [22 (link)]. Adipose tissue was minced and incubated during 35–40 min at 37 °C and constant mild shaking (10 rpm) in 5 mL of collagenase type I solution (PanEco, Moscow, Russia) 1 mg/mL in Krebs–Ringer buffer (2 mM D-glucose, 135 mM NaCl, 2.2 mM CaCl2·2H2O, 1.25 mM MgSO4·7H2O, 0.45 mM KH2PO4, 2.17 mM Na2HPO4, 25 mM HEPES, 3.5% BSA, 0.2 mM adenosine). Krebs–Ringer buffer (37 °C) was added to the digested tissue to neutralize collagenase in 1:1 ratio. The suspension of cells was filtered through the nylon mesh (Falcon™Cell strainer, Glendale, AZ, USA, 100 μm) and rinsed three times with warm Krebs–Ringer buffer. The numbers and size of the obtained adipocytes were calculated in a Goryaev cell chamber by light microscopy (Axio Observer.Z1, Carl Zeiss Surgical GmbH, Oberkochen, Germany). The representative images of EAT adipocytes are displayed in Figure 1. Cells were stained with Hoechst 33,342 (5 μg/mL, stains nucleus of viable cells) and propidium iodide (10 μg/mL, Sigma-Aldrich, St. Louis, MO, USA, stains nucleus of dead cells) to distinguish viable cells from dead cells [23 (link)]. Samples with viability lower than 95% were excluded from the study. The remaining samples did not differ significantly in the percentage of viable cells.
Kologrivova I.V., Naryzhnaya N.V., Koshelskaya O.A., Suslova T.E., Kravchenko E.S., Kharitonova O.A., Evtushenko V.V, & Boshchenko A.A. (2023). Association of Epicardial Adipose Tissue Adipocytes Hypertrophy with Biomarkers of Low-Grade Inflammation and Extracellular Matrix Remodeling in Patients with Coronary Artery Disease. Biomedicines, 11(2), 241.
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Publication 2023
Adenosine Adipocytes Buffers Cell Nucleus Cells Collagenase collagenase 1 Coronary Artery Bypass Surgery Glucose HEPES Lamina 1 Light Microscopy Nylons Operative Surgical Procedures Propidium Iodide Sodium Chloride Staining Sterility, Reproductive Sulfate, Magnesium Tissue, Adipose Tissues
2
Micromanipulation of TSPAN4-GFP GPMVs
Polydimethylsiloxane (PDMS) walls were placed on the bottom cover slip (Thorlabs CG15KH1) and mounted onto an automated XY-stage. The GPMVs sample was added to the chamber and after about 15 min, a few drops of oil were put on the sample surface to prevent evaporation. A micropipette aspiration setup including micromanipulator (Sensapex) holding a micropipette with diameter of 5 μm (BioMedical instruments) connected to a Fluigent EZ-25 pump was integrated into our optical tweezers instrument. Before each experiment, the zero-suction pressure was found by aspirating a 3.43 μm polystyrene bead (Spherotech) into the pipette and reducing the suction pressure until the bead stopped moving. A membrane tube was pulled from aspirated GPMVs using beads trapped by the optical tweezers. First, a membrane tube was pulled at relatively low suction pressure (0.05–0.1 mbar, correspond to 1.2–2 × 10−5 N/m membrane tension), then the suction pressure was reduced to zero (corresponds to zero applied membrane tension) for about 15 s. Then, we increased instantaneously the suction pressure to values in the range of 0.2–0.8 mbar (correspond to 2–15 × 10−5 N/m membrane tension) for HEK293T-GPMVs or 0.5–1.1 mbar (correspond to 10–25 × 10−5 N/m membrane tension). The control experiments were conducted on HEK293T-GPMVs and NRK-GPMVs (which did not contain TSPAN4-GFP). The same tension jumps used for TSPAN4-GPMVs were done on the GPMVs from the control group (±5 × 10−6 N/m). Microfluidics: To induce shear forces on TSPAN4-GPMVs, the GPMVs were injected at 1.5 bar into a 5-channel laminar flow cell (LUMICKS, Amsterdam, the Netherlands), which was on the C-trap® confocal fluorescence optical tweezers stage. The pressure was reduced to zero and following the settlement of the GPMVs at the bottom of the flow cell, they were scanned using a 488 nm laser at 5% laser power.
Dharan R., Huang Y., Cheppali S.K., Goren S., Shendrik P., Wang W., Qiao J., Kozlov M.M., Yu L, & Sorkin R. (2023). Tetraspanin 4 stabilizes membrane swellings and facilitates their maturation into migrasomes. Nature Communications, 14, 1037.
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Publication 2023
Cells Fluorescence Lamina 1 polydimethylsiloxane Polystyrenes Pressure Suction Drainage Tissue, Membrane
3
Electrophysiological Recordings in Neuroscience
All signals were first passed to a PZ-5 pre-amplifier (Tucker-Davis Technologies Inc., Alachua, FL, USA). A 128-channel RZ-2 Neurophysiological Recording System (Tucker-Davis Technologies Inc., Alachua, FL, USA) was then used to acquire tethered electrophysiological recordings. EEG and EMG signals were continuously sampled at 305 Hz and bandpass filtered between 0.1–100 Hz. Signals were then downsampled offline to 256 Hz via spline interpolation. Laminar probe channel signals were sampled at 25 kHz. Two signals were extracted from the laminar probe channels: decimated multiunit activity (MUA) and local field potential (LFP). Multiunit activity is the high frequency component of neural activity that contains the spiking of multiple neurons within the vicinity of an electrode. Decimation is a process for downsampling the MUA whilst retaining spiking activity by storing only the highest amplitude value, either negative or positive, recorded during a set time period. This means that if multiple neurons spike during that period, only the largest is stored, thus the majority of spikes in the decimated signal will originate from nearby neurons. The resulting signal will therefore have a high amplitude when nearby neurons are spiking and a low amplitude during periods of quiescence or when distant neurons are spiking. MUA was generated by bandpass filtering the laminar signals between 300 Hz and 5 kHz then decimating to 498 Hz by splitting the signal into segments of ~ 50 samples and storing the maximum/minimum amplitude of alternating segments as integers. LFP was generated by zero-phase distortion bandpass filtering the laminar signal between 0.1 and 100 Hz and downsampling to 256 Hz via spline interpolation. All offline manipulations and analyses were performed using MATLAB (version R2020a; The MathWorks Inc, Natick, MA, USA). Prior to vigilance state scoring, signals were transformed into European Data Format as previously reported (see [30 (link)]).
Harding C.D., Guillaumin M.C., Krone L.B., Kahn M.C., Blanco-Duque C., Mikutta C, & Vyazovskiy V.V. (2023). Detection of neuronal OFF periods as low amplitude neural activity segments. BMC Neuroscience, 24, 13.
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Publication 2023
Europeans Lamina 1 Nervousness Neurons Wakefulness
4
Transient Expression of BrERF2 and BrERF109 in Tobacco
The coding sequences (CDS) of BrERF2 and BrERF109 full-length without the stop codon were amplified and fused with the pCAMBIA1300 vector. The recombinant plasmids (35S:BrERF2-GFP and 35S:BrERF109-GFP) or the control vector (35S: GFP), and nuclear marker NLS-RFP (NLS, nuclear localization sequence) were co-transformed into Agrobacterium. Then, the Agrobacterium containing 35S:BrERF2-GFP, 35S:BrERF109-GFP or 35S: GFP was injected into the suction of 4- to 6-week-old tobacco (Nicotiana benthamiana) blade with a 1 mL syringe without needle. The GFP fluorescence was observed by Axioskop 2 Plus fluorescence microscope (Zeiss, Oberkochen, Germany) at 48 h of infiltration [20 (link)].
Yue L., Kang Y., Zhong M., Kang D., Zhao P., Chai X, & Yang X. (2023). Melatonin Delays Postharvest Senescence through Suppressing the Inhibition of BrERF2/BrERF109 on Flavonoid Biosynthesis in Flowering Chinese Cabbage. International Journal of Molecular Sciences, 24(3), 2933.
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Publication 2023
Agrobacterium Cloning Vectors Codon, Terminator Exons Fluorescence Lamina 1 Microscopy, Fluorescence Needles Nicotiana Plasmids Suction Drainage Syringes
5
Volumetric Muscle Loss Repair in Rats
Adult Sprague Dawley rats (n = 20) weighing ~ 350 g, were subjected to an established VML injury model67 (link). VML defects were created in the middle third of the Tibialis anterior (TA) muscle in the left leg by excising about 20% of the TA weight using an 8 mm biopsy punch. VML defect plugs were minced using a scalpel blade into 1 mm3 fragments and used to repair the VML injuries. Fascia and skin were closed using interrupted stiches with 5–0 Vicryl sutures (J463G, Ethicon). The contralateral limb was not injured and served as an uninjured control. Buprenorphine (0.1 mL at 0.3 mg/mL) (LIST 7571, Buprenex) was administered to all rats subcutaneously for postoperative analgesia and access to Rimadyl (MD150-2, Bio-Serv) was provided at up to 1 mg per day for seven days post-injury + repair (DPI). Food and water were provided ad libitum. All animal procedures were approved by the Institutional Animal Care and Use Committee of the University of Arkansas. All experiments were performed in accordance with all guidelines and ARRIVE guidelines. Animals were randomly assigned to one of two treatment groups (n = 10/treatment group): either rat recombinant IL-10 (400-19, PeproTech) in sterile phosphate buffered solution (PBS) (2000 ng/ml) or PBS injection (IM at site of repair) beginning 7 days after the initial surgery and continuing until day 14 (100 µL every other day = 4 injections total) (Fig. 1a,b). Animals (n = 5/treatment/time point) from each treatment group were allowed either 14 or 56 days of recovery.
Huynh T., Reed C., Blackwell Z., Phelps P., Herrera L.C., Almodovar J., Zaharoff D.A, & Wolchok J. (2023). Local IL-10 delivery modulates the immune response and enhances repair of volumetric muscle loss muscle injury. Scientific Reports, 13, 1983.
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Publication 2023
Adult Animals Biopsy Buprenex Buprenorphine Fascia Food IL10 protein, human Injuries Institutional Animal Care and Use Committees Lamina 1 Management, Pain Phosphates Rats, Sprague-Dawley Skin Sterility, Reproductive Sutures Tibial Muscle, Anterior Vicryl Wound Healing

Top products related to «Lamina 1»

1
Axopatch 200b amplifier by Molecular Devices
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The Axopatch 200B is a high-performance patch-clamp amplifier designed for electrophysiology research. It is capable of amplifying and filtering electrical signals from single-cell preparations, providing researchers with a tool to study ion channel and membrane properties.
2
Bx51wi by Olympus
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The BX51WI is an upright microscope designed for upright water immersion imaging. It features a infinity-corrected optical system and a bright LED illumination system. The BX51WI is suitable for a variety of applications requiring high-resolution imaging in an aqueous environment.
3
Digidata 1322a by Molecular Devices
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Axio imager a1 by Zeiss
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The Axio Imager A1 is a light microscope designed for advanced imaging and analysis applications. It features a robust and ergonomic design, providing users with a reliable and efficient tool for their research and investigation needs.
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Bx51 microscope by Olympus
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Clampex version 10 by Molecular Devices
Clampex version 10 is a data acquisition and analysis software developed by Molecular Devices for electrophysiology experiments. It provides a user-friendly interface for recording, analyzing, and managing electrophysiological data.
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Multiclamp 700b amplifier by Molecular Devices
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The Multiclamp 700B amplifier is a versatile instrument designed for electrophysiology research. It provides high-quality amplification and signal conditioning for a wide range of intracellular and extracellular recording applications. The Multiclamp 700B offers advanced features and precise control over signal acquisition, enabling researchers to obtain reliable and accurate data from their experiments.
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More about "Lamina 1"

Lamina I, dorsal horn layer 1, superficial dorsal horn, nociceptive processing, somatosensory integration, Axopatch 200B amplifier, BX51WI, Digidata 1322A, Axio Imager A1, BX51 microscope, Clampex version 10, Multiclamp 700B amplifier, VT1000S, ORCA-ER, Alexa Fluor 488.
Lamina 1, the most superficial layer of the dorsal horn in the spinal cord, plays a critical role in the processing and transmission of sensory information, including pain perception, temperature sensation, and touch.
Researchers studying the physiology and function of this important region can leverage advanced tools and techniques to optimize their investigations.
The Axopatch 200B amplifier, for example, is a widely used instrument for electrophysiological recordings, providing high-quality data capture from Lamina 1 neurons.
Paired with the BX51WI microscope and Digidata 1322A data acquisition system, researchers can precisely monitor and analyze the electrical activity of individual cells within this laminar structure.
Additionally, the Axio Imager A1 and BX51 microscope systems, coupled with Clampex version 10 software, enable detailed visualization and imaging of Lamina 1 anatomy and neuronal morphology.
The Multiclamp 700B amplifier, meanwhile, allows for sophisticated voltage- and current-clamp recordings, further advancing our understanding of the intricate signaling mechanisms within this critical spinal cord region.
Fluorescent labeling with Alexa Fluor 488 can also be employed to track and identify specific neuronal populations within Lamina 1, leveraging tools like the VT1000S vibratome and ORCA-ER camera for high-resolution imaging.
By utilizing these state-of-the-art techniques and instrumentation, researchers can unlock new insights into the complex sensory processing and integration happening within Lamina 1, ultimately driving progress in pain management, temperature regulation, and somatosensory function.