Log In Sign up
The largest database of trusted experimental protocols
> Anatomy > Body Part > Mesangiums, Glomerular

Mesangiums, Glomerular

Mesangiums, Glomerular: The mesangium is a specialized region within the glomerulus of the kidney.
It consists of mesangial cells and their surrounding extracellular matrix.
The mesangium plays a crucial role in regulating glomerular filtration and maintaining the structural integrity of the glomerulus.
Understanding the biology and pathology of the mesangium is important for the study of various kidney diseases, such as glomerulonephritis and diabetic nephropathy.
Reserchers can leverage PubCompare.ai's AI-powered platform to optimize their research protocols focused on this key component of the renal glomerulus.

Most cited protocols related to «Mesangiums, Glomerular»

1
Podocyte-Specific Gene Expression in Mice
The genome-wide expression data of murine podocytes, mesangial, and endothelial cells were obtained from http://www.gudmap.org/. The identifications of data sets that have been utilized in our study are listed in Supplemental Table 5. The detailed protocol of data generation is described in a recently published paper by Brunskill et al. (2011) (link) and can also be found in our Supplemental Methods. We preprocessed and normalized data as described in Microdissected Human Kidney Data. By comparing the expression level in podocytes versus the other two major cell types in glomeruli, we define a gene to be podocyte-specific if its expression in podocytes is 4.76-fold over mesangial cells and 4.65-fold over glomerular capillary endothelial cells. The cut-off values represent three standard deviations of the average difference between podocytes and mesangial/endothelial cell transcripts, respectively. By use of HomoloGene from NCBI Entrez (Maglott et al. 2011 (link)), 102 murine genes could be mapped to their Homo sapiens ortholog (Supplemental Table 6).
Ju W., Greene C.S., Eichinger F., Nair V., Hodgin J.B., Bitzer M., Lee Y.S., Zhu Q., Kehata M., Li M., Jiang S., Rastaldi M.P., Cohen C.D., Troyanskaya O.G, & Kretzler M. (2013). Defining cell-type specificity at the transcriptional level in human disease. Genome Research, 23(11), 1862-1873.
Publication 2013
Capillary Endothelial Cells Cells Endothelial Cells Endothelium Genes Genome Homo sapiens Kidney Kidney Glomerulus Mesangial Cells, Kidney Mesangiums, Glomerular Mus Podocytes
2
Histological Analysis of Kidney and Heart
Kidney and heart specimens (4 mm) fixed in 10% formalin, paraffin-embedded, and stained with Masson's trichrome and Periodic acid-Schiff (PAS) reagent (Sigma-Aldrich). Semi-qualitiative histologic changes were assessed by a masked observer. The total numbers of kidney glomeruli were counted, and the degree of glomerular damage was estimated at 400× magnification from the degree of mesangial expansion in PAS stained tissue as follow: <25%, 25–50%, 50–80%, >80% [79 (link)]. Kidney interstitial fibrosis was estimated at 200× magnification on Masson's trichrome-stained sections using 10 randomly selected fields for each animal by the following semiquantitative criteria: 0, area of damage <5%; 1, areas of damage 5–10%; 2, damage involving 10–25%; 3, damage involving 25–50%; 4, >50% of the area being affected [73 (link)]. The cardiac fibrosis area was determined at 200× magnification in Masson's trichrome stained sections using ImageJ 1.36b (National Institutes of Health, USA).
Leelahavanichkul A., Yan Q., Hu X., Eisner C., Huang Y., Chen R., Mizel D., Zhou H., Wright E.C., Kopp J.B., Schnermann J., Yuen P.S, & Star R.A. (2010). Rapid CKD progression in a new mouse kidney remnant model: strain-dependent resistance is overcome by angiotensin II. Kidney international, 78(11), 1136-1153.
Publication 2010
Animals Fibrosis Formalin Heart Kidney Kidney Glomerulus Mesangiums, Glomerular Paraffin Periodic Acid Schiff's reagent Tissue Stains
3
Quantitative Kidney Biopsy Analysis
A subset of subjects underwent percutaneous kidney biopsy at the end of the treatment period to determine whether treatment with losartan was associated with structural differences. Baseline kidney biopsies were not performed because of safety concerns. Tissue was processed and embedded in epoxy resin (Epon 812) and prepared for microscopy as described previously (12 (link)). Light and electron microscopy were performed either in the Beckman Center for Electron Microscopy at Stanford University or in the Division of Nephrology at the University of Minnesota. Digital light and electron micrographs were used to make measurements using formal stereologic methods to account for two-dimensional sampling of three-dimensional objects (13 ). Predefined morphometric variables included glomerular volume, percent globally sclerotic glomeruli, fractional interstitial area, mesangial fractional volume, filtration surface area density, glomerular basement membrane width, number of endothelial cells, mesangial cells and podocytes per glomerulus, filtration slit frequency, and foot process width (12 (link),14 (link)–16 (link)).
Weil E.J., Fufaa G., Jones L.I., Lovato T., Lemley K.V., Hanson R.L., Knowler W.C., Bennett P.H., Yee B., Myers B.D, & Nelson R.G. (2013). Effect of Losartan on Prevention and Progression of Early Diabetic Nephropathy in American Indians With Type 2 Diabetes. Diabetes, 62(9), 3224-3231.
Publication 2013
Biopsy Electron Microscopy Electrons Endothelium Epon 812 Epoxy Resins Filtration Fingers Foot Glomerular Basement Membrane Kidney Kidney Glomerulus Losartan Mesangial Cells, Kidney Mesangiums, Glomerular Microscopy Percutaneous Administration Podocytes Safety Sclerosis Tissues
4
Comprehensive Renal Histopathology Evaluation
Proteinuria was determined with Albustix strips (Bayer) using a 0–4 scale. Transverse sections of kidney were processed and stained with hemotoxylin and eosin (H&E) and periodic acid Schiff (PAS). Renal lesions were classified as previously described based on a modified International Society of Nephrology/Renal Pathology Societyclassification (15 (link)). Briefly glomerular lesions were classified: negative, mesangial matrix (PAS+) expansion (Mm), mesangial expansion with increased mesangial cellularity (Mc), capillary hyaline (immune complex type) deposits (HD) and proliferative glomerulonephritis (glomerular hypercellularity with capillary loop involvement, which was global [Pg] or segmental [Ps]). Extent of involvement was graded on a 1–4 scale. Glomerular sclerosis was graded separately. Chronic (mononuclear cell) inflammation was classified as perivascular or interstitial (ICI) and graded as for glomerular inflammation. Glomerular macrophages and CD4+ T cells weredetected on re-hydrated 8 µM sections first incubated with 0.6% H2O2 and 0.2% NaN3 to inhibit endogenous peroxidase activity and stained with biotin-conjugated anti-CD68 (Serotec) or anti-CD4 (BD Pharmingen). HRP-streptavidin (Vector) and DAB substrate were added and positive cells developed a brown color. Sections were counterstained with Mayer's hematoxylin solution (Sigma). The numbers of CD68+ or CD4+ cells were averaged from 10 randomly selected glomeruli under high power field for each kidney section. Detection of glomerular immune complex deposits were assessed on frozen sections stained with anti-IgG2a or anti-C3 FITC as previously described(15 (link)).Formalin-fixed inter-scapular skin sections were stained with H&E.
Xu Z., Cuda C.M., Croker B.P, & Morel L. (2011). The NZM2410-derived lupus susceptibility locus Sle2c1 increases TH17 polarization and induces nephritis in Fas-deficient mice. Arthritis and rheumatism, 63(3), 764-774.
Publication 2010
Biotin Capillaries Cardiac Arrest CD4 Positive T Lymphocytes Cells Cloning Vectors Complex, Immune Eosin Fluorescein-5-isothiocyanate Formalin Frozen Sections Glomerulonephritis Hyalin Substance Hyperplasia IgG2A Inflammation Kidney Kidney Glomerulus Macrophage Mesangial Cells, Kidney Mesangiums, Glomerular Periodic Acid Peroxidase Peroxide, Hydrogen Scapula Sclerosis Skin Sodium Azide Streptavidin
5
Kidney Histopathology and Spleen Apoptosis Assessment
Kidney (4 mm) was fixed in 10% formalin, paraffin embedded, and stained with Periodic acid–Schiff (PAS) stain (Sigma-Aldrich) for the semi-quantitative evaluation21 (link),22 (link). Glomerular injury was determined by percentage of moderate-severe glomerular injury (mesangial expansion >50%, crescentic formation and/or glomerulosclerosis) at 400x magnification and interstitial injury was semi-quantitatively estimated at 200x magnification using 10 randomly selected fields by the criteria of damage-area (cell infiltration, interstitial edema and tubular injuries) as following: 0, <5% area; 1, 5–10% area; 2, 10–25% area; 3, 25–50% area; and 4, >50% area. The immune complex deposition in glomeruli was visualized by immunofluorescence prepared in Cryogel (Leica Biosystems, Richmond, IL, USA), stained with goat anti-mouse IgG (Alexa Fluor 488, Abcam, Cambridge, MA, USA) and detected by ZEISS LSM 800 (Carl Zeiss, Germany). Spleen apoptosis was detected by immunohistochemistry with anti-active caspase 3 antibody (Cell Signaling Technology, Beverly, MA, USA) (expressed as positive cells per high-power field)22 (link) and flow cytometry. The fluorochrome-conjugated antibodies against different molecules were used including; i) apoptosis indicators, annexin V and propidium iodide (PI), ii) B220 (B cell) and iii) F4/80 (macrophage) (BioLegend, San Diego, CA, USA) with FlowJo software23 (link).
Thim-uam A., Surawut S., Issara-Amphorn J., Jaroonwitchawan T., Hiengrach P., Chatthanathon P., Wilantho A., Somboonna N., Palaga T., Pisitkun P, & Leelahavanichkul A. (2020). Leaky-gut enhanced lupus progression in the Fc gamma receptor-IIb deficient and pristane-induced mouse models of lupus. Scientific Reports, 10, 777.
Full text: Click here
Publication 2020
alexa fluor 488 Annexin A5 anti-IgG Antibodies Antibodies, Anti-Idiotypic Apoptosis B-Lymphocytes Caspase 3 Cells Complex, Immune Cryogels Edema Flow Cytometry Fluorescent Dyes Formalin Goat Immunofluorescence Immunohistochemistry Injuries Kidney Kidney Glomerulus Macrophage Mesangiums, Glomerular Mus Paraffin Periodic Acid Propidium Iodide Spleen

Most recents protocols related to «Mesangiums, Glomerular»

1
Immunohistochemical Analysis of Mesangial Cell Proliferation
Sections of 4–5 µm thickness were processed for immunohistochemistry as described by [30 (link)]. Briefly, the sections were deparaffinized, rehydrated, and rinsed in tap water. Then the slides were embedded in 3% hydrogen peroxide for 10 minutes, immersed in antigen retrieval solution and later blocking of nonspecific protein binding was done by incubation in 10% normal goat serum in PBS for 1 hours at room temperature. The slides were incubated with the diluted primary antibody against α- smooth muscle actin (Rabbit Polyclonal antibody, Cat: RB-9010-P0; Lab-Vision, Fremont, CA, USA) at dilution of 1:200 for 2 hours to identify mesangial cell proliferation in paraffin sections.
Moustafa A.H., Pasha H.F., Abas M.A, & Aboregela A.M. (2023). The ameliorating role of sofosbuvir and daclatasvir on thioacetamide-induced kidney injury in adult albino rats. Anatomy & Cell Biology, 56(1), 109-121.
Publication 2023
Actins Antigens Cardiac Arrest Cell Proliferation Goat Immunoglobulins Immunohistochemistry Mesangiums, Glomerular Paraffin Peroxide, Hydrogen Rabbits Serum Smooth Muscles Technique, Dilution Vision
2
Histological Assessment of Kidney Tissue
Kidney tissues were fixed in formalin, and the paraffin blocks were sliced into 4 µm sections. The kidney section was stained with PAS stain Kit (395B, Sigma-Aldrich) or H&E staining Kit (ab245880, Abcam, Cambridge, MA). Glomeruli area and mesangial matrix area were observed under a digital microscope. The mesangial matrix index was calculated by 100 × mesangial matrix area/glomeruli area. For assessing H&E staining results, the following factors were used to estimate tubulointerstitial lesions for HE staining: a, atrophy, expansion, and casts of tubules; b, tubulointerstitial fibrosis and infiltration of inflammatory cells. The specific grades varied from zero to four points: zero for no changes, one for changes of 25% or less in the section, two for changes of 25–50%, three for changes of 50–75%, and four for changes of 75–100%.
Hong Y., Wang J., Sun W., Zhang L., Xu X, & Zhang K. (2023). Gallic acid improves the metformin effects on diabetic kidney disease in mice. Renal Failure, 45(1), 2183726.
Full text: Click here
Publication 2023
Atrophy CD3EAP protein, human Cells Fibrosis Formalin Inflammation Kidney Kidney Glomerulus Mesangiums, Glomerular Microscopy Paraffin Stains Tissues
3
Gastric Bypass Surgical Procedures
The SPGJ procedure is illustrated in Figure 1. A separate part of the stomach at the junction of the gastric corpus and antrum or about 5 cm from the upper edge of the tumor with a linear stapler, leaving the 2–3 cm wide gastric corpus near the lesser curvature. Cut an opening at the margin of the greater curvature of the proximal stomach. Cut another opening on the opposing mesangial limbus of the jejunum 5–10 cm away from the ligament of flexion. Then a linear stapler is inserted, and the greater curvature and the jejunum are side-to-side anastomosed at the back of the colon. The opening was closed by continuous suture with barbed wire.
The CGJ procedure is illustrated in Figure 2. Cut an opening at the lowermost point of the greater curvature or about 5 cm from the upper edge of the tumor. Cut another opening on the opposing mesangial limbus of the jejunum 5–10 cm away from the ligament of flexion. Then a linear stapler is inserted, and the greater curvature and the jejunum are side-to-side anastomosed at the back of the colon. The opening was closed by continuous suture with barbed wire.
Zhang H., Xu F., Zheng Z., Liu X., Yin J., Fan Z, & Zhang J. (2023). Gastric emptying performance of stomach-partitioning gastrojejunostomy versus conventional gastrojejunostomy for treating gastric outlet obstruction: A retrospective clinical and numerical simulation study. Frontiers in Bioengineering and Biotechnology, 11, 1109295.
Full text: Click here
Publication 2023
Antral CM 2-3 Colon Jejunum Ligaments Mesangiums, Glomerular Neoplasms Stomach Sutures
4
Renal Biopsy and Histological Assessment of IgA Nephropathy
Renal biopsy specimens were collected via percutaneous needle biopsy. Paraffin-embedded 3-μm thick sections of renal specimens were stained with periodic acid Schiff, silver methenamine, and Masson trichrome. For the immunofluorescence analysis, frozen sections were subjected to fluorescence by fluorescein-conjugated goat IgG fraction to human IgG (F110FC, American Qualex, San Clemente, CA, USA), fluorescein-conjugated goat IgG fraction to human IgA (55077, MP Biomedicals, Solon, OH, USA), fluorescein-conjugated goat IgG fraction to human IgM (55153, MP Biomedicals), fluorescein-conjugated goat IgG fraction to human C3 (55167, MP Biomedicals), fluorescein-conjugated rabbit anti-human C1q (F0254, DAKO Japan Inc., Kyoto, Japan), and fluorescein-conjugated goat IgG fraction to human fibrinogen (55169, MP Biomedicals). IgAN or IgA vasculitis was diagnosed on the basis of mesangial cell proliferation in light microscopic findings, mesangial IgA deposition in immunofluorescence findings, and mesangial electron dense deposits in electron microscopic findings. Histological findings were evaluated according to the Oxford classification [39 (link)–41 (link)]. The Oxford classification of IgAN includes the following five histological variables: mesangial hypercellularity (M0/M1 lesion), segmental glomerulosclerosis (S0/S1 lesion), endocapillary hypercellularity (E0/E1 lesion), tubular atrophy/interstitial fibrosis (T0/T1/T2 lesion), and crescents (C0/C1/C2 lesion) [41 (link)]. We compared the renal histology in the cnm-positive S. mutans group with that in the cmn-negative S. mutans group using the Oxford classification in a blind test.
Misaki T., Naka S., Suzuki H., Lee M., Aoki R., Nagasawa Y., Matsuoka D., Ito S., Nomura R., Matsumoto-Nakano M., Suzuki Y, & Nakano K. (2023). cnm-positive Streptococcus mutans is associated with galactose-deficient IgA in patients with IgA nephropathy. PLOS ONE, 18(3), e0282367.
Full text: Click here
Publication 2023
Atrophy Biopsy Blindness Electron Microscopy Fibrinogen Fibrosis Fluorescein Fluorescence Fluorescent Antibody Technique Frozen Sections Goat Henoch-Schoenlein Purpura Homo sapiens Hyperplasia IGA Glomerulonephritis Kidney Light Microscopy Mesangial Cells, Kidney Mesangiums, Glomerular Paraffin Embedding Periodic Acid Puncture Biopsy Rabbits Segmental glomerulosclerosis Solon
5
Mesangial Cell Culture Conditions
Human renal mesangial cells (HRMCs) were purchased from ScienCell Research (San Diego, United States), and the mouse mesangial cell (MMC) line SV40 MES-13 was purchased from the American Type Culture Collection (ATCC). All cells were cultured in DMEM (Gibco, United States) containing 10% fetal bovine serum (Gibco, Australia), streptomycin (100 μg/mL) and penicillin (100 U/mL) at 37°C and 5% CO2. Standard medium containing 5 mM D-glucose was used for the normal glucose (NG) condition. We added 20 mM glucose (yielding a final concentration of 25 mM) or mannitol for the high glucose (HG) condition or osmotic control (MA) and stimulated the cells for 48 h. The concentration was chosen based on our previous studies (Lu et al., 2015 (link); Wang et al., 2018a (link); Ma et al., 2019 (link)).
Li X., Ma T.K., Wang M., Zhang X.D., Liu T.Y., Liu Y., Huang Z.H., Zhu Y.H., Zhang S., Yin L., Xu Y.Y., Ding H., Liu C., Shi H, & Fan Q.L. (2023). YY1-induced upregulation of LncRNA-ARAP1-AS2 and ARAP1 promotes diabetic kidney fibrosis via aberrant glycolysis associated with EGFR/PKM2/HIF-1α pathway. Frontiers in Pharmacology, 14, 1069348.
Full text: Click here
Publication 2023
Cell Lines Fetal Bovine Serum Glucose Homo sapiens Mannitol Mesangial Cells, Kidney Mesangiums, Glomerular Mus Osmosis Penicillins Simian virus 40 Streptomycin

Top products related to «Mesangiums, Glomerular»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Image pro plus 6 by Media Cybernetics
Sourced in United States, Japan, Germany, United Kingdom, China, Hungary, Singapore, Canada, Switzerland
Image-Pro Plus 6.0 is a comprehensive image analysis software package designed for scientific and industrial applications. It provides a wide range of tools for image capture, enhancement, measurement, analysis, and reporting.
3
Sv40 mes 13 by American Type Culture Collection
Sourced in United States, Japan
The SV40 MES 13 is a laboratory equipment product offered by American Type Culture Collection. It is designed for cell culture applications, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.
4
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
5
Ax10 microscope by Zeiss
Sourced in Germany, Canada, United States, United Kingdom
The Zeiss AX10 microscope is a high-performance optical instrument designed for various laboratory applications. It features a sturdy, ergonomic design and delivers precise imaging capabilities. The AX10 microscope is equipped with advanced optics and illumination systems to enable clear and detailed observations of samples.
6
Image pro plus by Media Cybernetics
Sourced in United States, Japan, United Kingdom, Germany, China, Canada, Switzerland, Moldova, Republic of
Image-Pro Plus is a comprehensive software solution for advanced image analysis and processing. It provides a suite of tools for image capture, enhancement, measurement, and analysis. The software is designed to work with a wide range of microscopy, imaging, and digital photography equipment, making it a versatile tool for researchers, scientists, and professionals in various fields.
7
Lipofectamine 2000 by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Lithuania, Hong Kong, Argentina, Ireland, Austria, Israel, Czechia, Cameroon, Taiwan, Province of China, Morocco
Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
8
Bx43 microscope by Olympus
Sourced in Japan, United States, Germany, China, United Kingdom, Spain
The Olympus BX43 is a high-performance optical microscope designed for a wide range of applications. It features a sturdy and durable construction, with a built-in illumination system and a range of objective lenses to accommodate various magnification requirements. The BX43 is equipped with a sturdy, ergonomic design that promotes comfortable operation during extended use.
9
Cryo nhmc by Cambrex
Sourced in United States
The Cryo NHMC is a laboratory instrument designed for the cryogenic cooling and temperature control of samples. It provides precise temperature regulation and maintains samples at low temperatures, enabling research and analysis in a wide range of scientific applications.
10
Penicillin streptomycin by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia
Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

More about "Mesangiums, Glomerular"

Mesangial cells play a vital role in the glomerular filtration process within the kidneys.
This specialized region, known as the mesangium, consists of mesangial cells and the surrounding extracellular matrix.
The mesangium is crucial for regulating glomerular filtration and maintaining the structural integrity of the glomerulus, the functional unit of the kidney.
Understanding the biology and pathology of the mesangium is essential for the study of various kidney diseases, such as glomerulonephritis and diabetic nephropathy.
Researchers can leverage the power of PubCompare.ai's AI-powered platform to optimize their research protocols focused on this key component of the renal glomerulus.
PubCompare.ai's innovative tool helps researchers locate the best protocols from literature, pre-prints, and patents through intelligent comparisons and analysis.
This cutting-edge technology can unlock the potential of research by providing researchers with the ability to explore and compare various experimental methodologies, including the use of FBS, Image-Pro Plus 6.0, SV40 MES 13 cells, DMEM, AX10 microscope, Image-Pro Plus software, Lipofectamine 2000, BX43 microscope, Cryo NHMC, and Penicillin/streptomycin.
By leveraging PubCompare.ai's AI-powered platform, researchers can streamline their investigations and gain valuable insights into the mesangial cells and the glomerular filtration process, ultimately advancing our understanding of kidney health and disease.