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Muscularis Mucosae

The Muscularis Mucosae is a thin layer of smooth muscle located within the mucosa of the gastrointestinal tract.
This layer plays a key role in regulating the movement and mixing of contents within the gut.
Disturbances in the Muscularis Mucosae can contribute to various gastrointestinal disorders, makeing it an important target for research.
PubCompare.ai's AI-driven analysis can enhance your Muscularis Mucosae studies by helping you easily locate relevant protocols from literature, preprints, and patents, while leveraging AI comparisons to identify the best methodologies and products.
Improve reproducibility and accuaracy in your Muscularis Mucosae research with PubCompare.ai's powerful tools.

Most cited protocols related to «Muscularis Mucosae»

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Publication 2012
Animals Bath Brain Cells Connective Tissue Decellularized Extracellular Matrix Deoxycholate Edetic Acid Ethanol Freeze Drying Freezing Membrane, Basement Muscularis Mucosae Peracetic Acid Pigs Serous Membrane Spinal Cord Sucrose Tissues Tissue Specificity Triton X-100 Trypsin Urinary Bladder Urothelium
UBM was prepared as previously described (66 (link)). Porcine urinary bladders from market-weight animals were acquired from Tissue Source, LLC. Briefly, the tunica serosa, tunica muscularis externa, tunica submucosa, and tunica muscularis mucosa were mechanically removed. The luminal urothelial cells of the tunica mucosa were dissociated from the basement membrane by washing with deionized water. The remaining tissue consisted of basement membrane and subjacent lamina propria of the tunica mucosa and was decellularized by agitation in 0.1% peracetic acid with 4% ethanol for 2 hours at 300 rpm. The tissue was then extensively rinsed with PBS and sterile water. The UBM was then lyophilized and milled into particulate form using a Wiley Mill with a #60 mesh screen.
Publication 2016
Adventitia Animals Bladder Detrusor Muscle Ethanol Lamina Propria Membrane, Basement Mucous Membrane Muscularis Mucosae Peracetic Acid Serous Membrane Sterility, Reproductive Tissues Urinary Bladder Urine Urothelium

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Publication 2005
CFC1 protein, human Enterocytes Formalin Groin In Situ Hybridization Jejunum Muscularis Mucosae Nodes, Lymph Virus
Specimens of the distal ileum were collected from each group of animals at the end of the reperfusion period to determine the level of tissue damage. Following overnight fixation in 10% formalin, samples of ileum were embedded in paraffin. Sections (4 µm) were stained with hematoxylin and eosin. Microscopic histological damage score was evaluated by a person unaware of the treatments and was based on a semi-quantitative scoring system in which the following features were graded: damage of epithelium (0 morphologically normal; 1 development of subepithelial space; 2 presence of subepithelial space with moderate lifting of epithelial layer; 3 severe epithelial lifting), inflammatory cells infiltration (0 absence of infiltrate or less than five cells; 1 mild infiltration to the lamina propria; 2 moderate infiltration to the muscularis mucosae and submucosa; 3 severe transmural infiltration involving the muscle layer), extent of muscle thickening (0 normal; 1 moderate; 2 severe), edema (0 no edema; 1 zonal edema in lamina propria and submucosa; 2 diffuse edema in lamina propria and submucosa). Data were expressed as sum of scores for each feature (total score).
Publication 2012
Animals Bladder Detrusor Muscle Cells Edema Eosin Epithelium Formalin Ileum Inflammation Lamina 1 Lamina Propria Microscopy Muscle Tissue Muscularis Mucosae Paraffin Embedding Reperfusion Tissues Zonal
On the basis of a comprehensive literature review, including a prior systematic review,20 (link) and clinical relevance, we identified that the following variables were potentially associated with bleeding after ESD: sex, comorbidities (ischaemic heart disease, liver cirrhosis and chronic kidney disease (CKD) with haemodialysis), AT therapy (aspirin, P2Y12RA, cilostazol, warfarin and DOAC), the tumour characteristics (multiple tumours, lower-third in tumour location, tumour size >30 mm, undifferentiated type, submucosal invasion ≥500 µm from the muscularis mucosa (SM2) and ulceration) and procedure (ESD procedure time >120 min and SLE). We also selected characteristics that may influence bleeding, including age (≥75 years), piecemeal resection and mild/no endoscopic gastric atrophy according to the Kimura and Takemoto classification.21 (link) With regard to the AT agents, the variables for the interruption of AT agents, heparin bridging and replacement of APAs were included in the model because of their possible influence on bleeding.
Publication 2020
Aspirin Chronic Kidney Diseases Cilostazol Endoscopy Gastritis, Atrophic Hemodialysis Heparin Liver Cirrhosis Muscularis Mucosae Myocardial Ischemia Neoplasms Neoplasms by Site Therapeutics Ulcer Warfarin

Most recents protocols related to «Muscularis Mucosae»

Subcutaneous adipose tissue depots were fixed in 4% paraformaldehyde for 24 h at room temperature. Samples were then immersed in ethanol 100% before processing for paraffin embedding. To determine the adipocyte diameter, paraffin sections of 5 µm were stained with hematoxylin and eosin. Images were obtained using a SCN400 slide scanner and digital Image Hub software 561 (Leica Biosystems, Wetzlar, Germany). Adipocyte diameter was determined using ImageJ (National institutes of health, Bethesda, MD, USA). F4/80 positive areas in the adipose tissue were randomly counted after immunostaining with F4/80 antibody (Ab6640, Abcam, Cambridge, UK). All histological observations were analyzed in a blinded manner by three individuals. At least 5 high-magnification fields/mice were randomly selected and obtained using SCN400 slide scanner and digital image hub software (Leica Biosystems, Wetzlar, Germany).
Analysis of the mucus layer and goblet cells was made as previously described.89 (link) Briefly, paraffin sections of 5 μm were stained with alcian blue. Images were captured at ×20 magnification and obtained using a SNC400 slide scanner and digital Image Hub software 561 (Leica Biosystems, Wetzlar, Germany). Analyses were performed using ImageJ (version 1.48 r, National Institutes of Health, Bethesda, Maryland, USA) in a blinded manner. For the mucus layer thickness, two to four fields were used for each mouse and a minimum of 50 different measurements (up to 200) were made perpendicular to the inner mucus layer per field. Each value represents the mean of the different measurements per field. For the goblet cells, the luminal side, muscularis mucosae, submucosa, and muscle layer were removed using ImageJ software and the blue area and the total area were measured separately in the remaining mucosal part of the colon. The proportion of the goblet cells was quantified based on the ratio between the blue area over the total area.
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Publication 2023
Adipocytes Alcian Blue Bladder Detrusor Muscle Colon Eosin Ethanol Goblet Cells Immunoglobulins Mucous Membrane Mucus Mus Muscularis Mucosae Paraffin paraform Phenobarbital Subcutaneous Fat Tissue, Adipose
For histological analysis, cross sectional images of the proximal, mid, and distal small intestine and colon were captured by an EVOSTM 7000 Imaging System at a 10× magnification. Invitrogen Celleste Image Analysis Software (SKU# AMEP4816) was then used for villi and crypt morphometric evaluation. The lines feature was used to measure villi height from the top of villus to the top border of muscularis mucosae, while the crypt depth was measured form the top to the base of the crypt. Eleven to eighteen measurements were taken per mouse. An average for each mouse was then determined. The number of goblet cells was counted in both villi and crypt of distal small intestine and crypt of colon. The stained goblet cells were counted from three individual regions for each mouse and the average cell number per villi or crypt was then calculated.
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Publication 2023
CFC1 protein, human Colon Goblet Cells Intestines, Small Mus Muscularis Mucosae
Endoscopic submucosal dissection (ESD) is the most common treatment option for gastrointestinal neoplasm, including EGC.22, 23 ESD was performed in patients with absolute indications, including intramucosal differentiated‐type adenocarcinoma measuring <2 cm without ulceration, and also in those with expanded indications. The expanded indications included (i) mucosal cancer without ulcer findings, irrespective of tumor size; (ii) mucosal cancer with an ulcer ≤3 cm in diameter; and (iii) minimal (≤500 μm from the muscularis mucosa) submucosal invasive cancer ≤3 cm in size.24, 25 The shape and margin of these lesions were determined, and the endoscopic procedures were performed using a single‐channel endoscope (GIF H260; Olympus, Tokyo, Japan). Using argon plasma coagulation, the lesion boundary was marked with dotted lines. Isotonic saline with dilute epinephrine (1:10 000) was then injected into the submucosal layer to elevate the lesion. For ESD, a circumferential incision was made around the lesion, which was dissected using an insulated tipped knife (or dual knife; Olympus). For sedation, 3–5 mg of midazolam was administered intravenously. All patients were monitored for cardiopulmonary functions.
Publication 2023
Adenocarcinoma Argon Plasma Coagulation Endoscopes Endoscopic Submucosal Dissection Epinephrine Gastrointestinal Neoplasms Malignant Neoplasms Midazolam Mucous Membrane Muscularis Mucosae Neoplasms Patients Saline Solution Sedatives Surgical Endoscopy Technique, Dilution Ulcer
We obtained partial sections of lung tissues and colon tissues. We routinely fixed the tissue with 4% paraformaldehyde and then gradient dehydration. The samples were cut, embedded in paraffin, bleached, and stained in hematoxylin and eosin (H&E). The score of lung injury and colon injury was performed by other investigators who were blinded to the study. For the lung injury, scoring principles were as follows: (1) alveolar congestion, (2) hemorrhage, (3) infiltration of neutrophils in the airspace or vessel wall, and (4) thickness of the alveolar wall formation. Each lung injury item was scored as follows: no damage or minimal damage = 0, mild damage = 1, moderate damage = 2, severe damage = 3, and diffuse injury = 4. Then, the lung injury was scored according to previous study and all scores were added up to obtain a total score [23 (link), 24 (link)]. Besides, the total histological score for colon injury ranges from 0 to 8 according to previous study as follows: epithelium: normal morphology = 0, loss of goblet cells = 1, loss of goblet cells in large areas = 2, loss of crypts = 3, and loss of crypts in large areas = 4; infiltration: no infiltrate = 0, infiltrate around crypts = 1, infiltrate into the lamina muscularis mucosae = 2, extensive infiltration into the lamina muscularis mucosae and thickening of the mucosa = 3, and infiltration of the submucosal layer = 4 [25 (link)].
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Publication 2023
Blood Vessel CFC1 protein, human Colon Dehydration Eosin Epithelium Goblet Cells Hemorrhage Injuries Lung Lung Injury Mucous Membrane Muscularis Mucosae Neutrophil Infiltration Paraffin Embedding paraform Tissues
All pathological slides of neoplasia were re-reviewed and classified in accordance with the most recent World Health Organization classification [30 ] by two expert gastrointestinal pathologists (YA and SC); one of whom (YA) was highly specialized in UC-associated dysplasia or cancer.
We classified neoplasia into five categories on the basis of the histological findings and whether the neoplasia was located in colitis-affected segments, as follows: serrated polyps in colitis-affected segments, conventional dysplasia (intestinal-type dysplasia in colitis-affected segments), serrated polyps in colitis-unaffected segments (usually proximal to the extent of the colitis), sporadic adenomas in colitis-unaffected segments, and invasive carcinoma. Additionally, serrated polyps in colitis-affected segments were histologically classified into three subtypes in accordance with previous studies [27 (link)–29 (link)], as follows: SSL-like dysplasia, TSA-like dysplasia, and serrated SD NOS. Briefly, SSL-like dysplasia is characterized by distorted serrated crypts with prominent basal crypt dilatation (i.e., dilated L- or inverted T-shaped crypts) at the interface with the muscularis mucosa. TSA-like dysplasia is characterized by a villiform growth pattern with columnar cells with intensely eosinophilic cytoplasm and ectopic crypts, creating a prominent serrated profile. SD NOS was defined as serrated dysplasia without definite features of SSL-like or TSA-like dysplasia, with a complex serrated architecture and evidence of dysplasia [27 (link)–29 (link)]. The representative endoscopic and histological features of SSL-like dysplasia, TSA-like dysplasia, and SD NOS are shown in S1 Fig. Serrated polyps in colitis-unaffected segments were classified as hyperplastic polyps, SSLs, TSAs, or unclassified serrated adenomas [30 ].
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Publication 2023
Adenoma Carcinoma Cells CFC1 protein, human Colitis Cytoplasm Endoscopy Eosinophilia Hyperplasia Intestines Malignant Neoplasms Muscularis Mucosae Neoplasms Pathologists Polyps theasinensin A

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More about "Muscularis Mucosae"

The Muscularis Mucosae is a thin layer of smooth muscle located within the mucosal lining of the gastrointestinal tract.
This important structure plays a crucial role in regulating the movement and mixing of contents within the gut.
Disturbances or abnormalities in the Muscularis Mucosae can contribute to various gastrointestinal disorders, making it a key target for research and investigation.
Researchers studying the Muscularis Mucosae may leverage a variety of microscopy techniques and tools, such as the BX51 microscope, HALO 2.3 platform, Microcomp, NanoZoomer, BX43 light microscope, and Eclipse 80i.
These instruments can be used to visualize and analyze the structure and function of the Muscularis Mucosae at different scales.
Additionally, sample preparation techniques involving microtomes, Sodium deoxycholate, SDS, and Triton X-100 may be employed to prepare tissue specimens for examination.
By utilizing the insights and capabilities of PubCompare.ai's AI-driven analysis, researchers can enhance their Muscularis Mucosae studies.
The platform can help easily locate relevant protocols, methodologies, and products from literature, preprints, and patents, while also providing AI-powered comparisons to identify the most effective approaches.
This can ultimately improve the reproducibility and accuracy of Muscularis Mucosae research, leading to a better understanding of this critical gastrointestinal structure and its role in various health and disease states.