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Quadriceps Femoris
The Quadriceps Femoris is a large muscle group in the anterior thigh, composed of four distinct muscle bellies: the rectus femoris, vastus lateralis, vastus medialis, and vastus intermedius.
This muscle group is responsible for knee extension and hip flexion, playing a critical role in ambulation and functional movement.
Researchers can leverage PubCompare.ai's AI-driven platform to effortlessly locate the best experimental protocols from literature, preprints, and patents, allowing them to identify the most accurate and reproducible methods to enhance their Quadriceps Femoris research outcomes.
With the platform's comparison tools, scientists can optimise their studies and unlock new insights into this important muscle group.
This muscle group is responsible for knee extension and hip flexion, playing a critical role in ambulation and functional movement.
Researchers can leverage PubCompare.ai's AI-driven platform to effortlessly locate the best experimental protocols from literature, preprints, and patents, allowing them to identify the most accurate and reproducible methods to enhance their Quadriceps Femoris research outcomes.
With the platform's comparison tools, scientists can optimise their studies and unlock new insights into this important muscle group.
Most cited protocols related to «Quadriceps Femoris»
Acceleration
Adult
Biceps Femoris
Cadaver
Cerebral Palsy
Child
Epistropheus
Femur
Foot
Generic Drugs
Gomphosis
Gravitation
Gravity
Head
Hip Joint
Joints
Joints, Ankle
Knee Joint
Muscle, Gastrocnemius
Muscle Tissue
Pelvis
Plant Roots
Quadriceps Femoris
Rectus Femoris
Semimembranosus
Tibia
Torso
Vastus Intermedius
Vastus Lateralis
Vastus Medialis
Vertebrae, Lumbar
Mice were anesthetized via inhalation isoflurane (2%). The surgical procedure was modified from previous work [49] (link), [50] (link). A skin incision was made over the right knee (Figure 1A ). The distal right femur was accessed through a medial parapatellar arthrotomy with lateral displacement of the quadriceps-patellar complex (Figure 1B ). After locating the femoral intercondylar notch (Figure 1B ), the femoral intramedullary canal was manually reamed with a 25 gauge needle (Figure 1C ). An orthopaedic-grade stainless steel Kirschner (K)-wire (diameter 0.6 mm) (Synthes) was surgically placed in a retrograde fashion and cut with 1 mm protruding into the joint space (Figure 1D ). An inoculum of S. aureus in 2 µl of normal saline was pipetted into the joint space containing the cut end of the implant (Figure 1E ). The quadriceps-patellar complex was reduced to the midline (Figure 1F ) and the surgical site was closed with Dexon 5-0 sutures (Figure 1G ). A representative radiograph demonstrates the position of the implant with good intramedually fixation of the stem and prominence of the cut surface in the joint (Figure 1H ). Buprenorphine (0.1 mg/kg) was administered subcutaneously every 12 hours as an analgesic for the duration of the experiment.
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Analgesics
Buprenorphine
dexon (fungicide)
Femur
Inhalation
Isoflurane
Joints
Kirschner Wires
Knee Joint
Mus
Needles
Normal Saline
Operative Surgical Procedures
Patella
Pulp Canals
Quadriceps Femoris
Skin
Stainless Steel
Stem, Plant
Sutures
X-Rays, Diagnostic
CASP3 protein, human
CD29 Antigen
Cells
CXCR4 protein, human
isolation
Macrophage-1 Antigen
Mus
Muscle, Gastrocnemius
Muscle Tissue
Population Group
Quadriceps Femoris
Soleus Muscle
Twelve mice (six male, six female) were selected at random from a single litter of CD1 wild-type mice at six weeks of age. Following euthanasia by overdose of inhaled isoflurane, each mouse was weighed, and the chosen muscles from each side were dissected out, within 30 min post-mortem. Muscles were immediately fixed in 4% paraformaldehyde for 30 min, then washed in 1% phosphate buffered saline (PBS). All remaining connective tissue was then removed. Cranial and lumbrical muscles intended for whole-mount were immediately prepared for immunohistochemistry (see below). The triceps and quadriceps were cryoprotected by immersion in 30% sucrose overnight; 100 µm sections were then obtained on a Thermo Scientific Microm HM 450/KS 34 freezing microtome.
Neuromuscular junctions were immunohistochemically labelled using a standard laboratory protocol for visualizing pre-synaptic 2H3/SV2 and post-synaptic AChRs [26 (link)]. Muscle preparations were placed in the following sequence of solutions (made up in 1% PBS unless otherwise specified; antibodies and their concentrations are listed below): α-bungarotoxin (BTX) for 30 min to label post-synaptic AChRs; 4% Triton X for 90 min; a blocking solution of 4% bovine serum albumin (BSA) and 2% Triton X for 30 min; the primary antibodies (made up in blocking solution) for 72 h at 4°C; 1% PBS for 80 min; 4% BSA for 30 min; the secondary antibodies (made up in 1% PBS) for 150 min; 1% PBS for 80 min. Finally, muscles preparations were mounted on glass slides in Mowiol, and stored at −20°C. At all stages, samples were protected from excessive light exposure prior to imaging.
Neuromuscular junctions were immunohistochemically labelled using a standard laboratory protocol for visualizing pre-synaptic 2H3/SV2 and post-synaptic AChRs [26 (link)]. Muscle preparations were placed in the following sequence of solutions (made up in 1% PBS unless otherwise specified; antibodies and their concentrations are listed below): α-bungarotoxin (BTX) for 30 min to label post-synaptic AChRs; 4% Triton X for 90 min; a blocking solution of 4% bovine serum albumin (BSA) and 2% Triton X for 30 min; the primary antibodies (made up in blocking solution) for 72 h at 4°C; 1% PBS for 80 min; 4% BSA for 30 min; the secondary antibodies (made up in 1% PBS) for 150 min; 1% PBS for 80 min. Finally, muscles preparations were mounted on glass slides in Mowiol, and stored at −20°C. At all stages, samples were protected from excessive light exposure prior to imaging.
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alpha-Bungarotoxin
Antibodies
Autopsy
Cardiac Arrest
Connective Tissue
Cranium
Drug Overdose
Euthanasia
Females
Immunohistochemistry
Isoflurane
Light
Males
Mice, House
Microtomy
Muscle Tissue
Neuromuscular Junction
paraform
Phosphates
Quadriceps Femoris
Saline Solution
Serum Albumin, Bovine
Submersion
Sucrose
Homo sapiens
Pharmaceutical Preparations
Physical Therapist
Quadriceps Femoris
Most recents protocols related to «Quadriceps Femoris»
Example 6
Increasing the Rate of Muscle Hypertrophy: Using the standard protocol, described above, subjects are instructed to follow a diet and exercise regimen for 4 weeks, including resistance training three days per week. At the completion of the study, the circumference of subjects' biceps, quadriceps, and chest are measured. The test group shows an average increase in circumference of about 5% relative to the control group.
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Chest
Chromium
Diet
Hypertrophy
Muscle Tissue
Quadriceps Femoris
Treatment Protocols
Example 6
Increasing the Rate of Muscle Hypertrophy: Using the standard protocol, described above, subjects are instructed to follow a diet and exercise regimen for 4 weeks, including resistance training three days per week. At the completion of the study, the circumference of subjects' biceps, quadriceps, and chest are measured. The test group shows an average increase in circumference of about 5% relative to the control group.
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Chest
Chromium
Diet
Hypertrophy
Muscle Tissue
Quadriceps Femoris
Treatment Protocols
The inclusion criteria were primary gout arthritis and were based on the gout diagnostic criteria of the 1977 American College of Rheumatology (ACR). All patients met the criteria for classifying primary gout arthritis. Patients with rheumatoid, reactive, psoriatic, spinal, or other inflammatory arthritis were excluded. Patients with gout arthritis received anti-inflammatory therapy during an acute episode and a standardized ULT in remission. All patients received a small dose of oral anti-inflammatory drugs during ULT to prevent paroxysm of gout arthritis. During treatment, SUA was measured monthly in all participants. SUA values of all participants were plotted as a continuous curve, and all gout patients were divided into treat-to-target (TTG) and treat-to-non-target (TNTG) groups. The TTG standard curve showed a decreasing trend; the lowest SUA was below 300 µmol/l. Other gout patients who did not meet the TTG criteria were included in the TNTG group (Figure 1 ).
All US investigations were performed by a doctor who had received formal musculoskeletal US training, using a Supersonic Imagine Aixplorer machine (French) with an SL15-4 linear array probe. Joints of both lower extremities (bilateral knee, ankle, tarsal, and metatarsophalangeal joints) and some tendons of both lower extremities (bilateral quadriceps tendon, pes anserinus tendon, patellar ligament, biceps femoris tendon, iliotibial band tibial attachment, and pollicis abductor tendon) were regularly checked. The MSUS examination was performed before treatment (M0), three months after treatment (M3), six months after treatment (M6), and 12 months after treatment (M12), and the DCS and tophus ultrasound images were recorded and stored for further use. DCS was scored based on the semiquantitative ultrasound scoring system (SQUS) of the Outcome Measures in Rheumatology Clinical Trials (OMERACT) in 2021 double contour sign [(DCS)-SQUS]. The DCS-SQUS was divided into four grades as follows: 0: not at all; 1: possible; 2: definite, minor; 3: definite, severe. The maximum long diameter and the short diameter of tophus were measured based on two-dimensional ultrasound images, and the maximum area of tophus was determined using Image J. The SQUS-DCS of ultrasound images was assessed separately by two musculoskeletal ultrasonographers using a blinded method, and in case of disagreement, the two physicians consulted and reached a consensus.
All US investigations were performed by a doctor who had received formal musculoskeletal US training, using a Supersonic Imagine Aixplorer machine (French) with an SL15-4 linear array probe. Joints of both lower extremities (bilateral knee, ankle, tarsal, and metatarsophalangeal joints) and some tendons of both lower extremities (bilateral quadriceps tendon, pes anserinus tendon, patellar ligament, biceps femoris tendon, iliotibial band tibial attachment, and pollicis abductor tendon) were regularly checked. The MSUS examination was performed before treatment (M0), three months after treatment (M3), six months after treatment (M6), and 12 months after treatment (M12), and the DCS and tophus ultrasound images were recorded and stored for further use. DCS was scored based on the semiquantitative ultrasound scoring system (SQUS) of the Outcome Measures in Rheumatology Clinical Trials (OMERACT) in 2021 double contour sign [(DCS)-SQUS]. The DCS-SQUS was divided into four grades as follows: 0: not at all; 1: possible; 2: definite, minor; 3: definite, severe. The maximum long diameter and the short diameter of tophus were measured based on two-dimensional ultrasound images, and the maximum area of tophus was determined using Image J. The SQUS-DCS of ultrasound images was assessed separately by two musculoskeletal ultrasonographers using a blinded method, and in case of disagreement, the two physicians consulted and reached a consensus.
Aftercare
Ankle
Anti-Inflammatory Agents
Arthritis
Arthritis, Gouty
Diagnosis
Gout
Joints
Knee
Ligamentum Patellae
Lower Extremity
Maple Syrup Urine Disease
Metatarsophalangeal Joint
Patients
Physicians
Quadriceps Femoris
Tendons
Tendons, Biceps Femoris
Tibia
Ultrasonics
The main objective of this study was to examine the relationship between marathon performance and changes in muscles stiffness from pre-to post-marathon in recreational runners aged 50+ years. The muscle stiffness of the quadriceps was measured in two independent sessions: the day before the marathon and 30 min after the completed marathon. Myoton measurements of each muscle group (12 points) were taken separately for the left and right legs.
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Leg
Marathon composite resin
Muscle Tissue
Quadriceps Femoris
The first muscle stiffness sample was collected on the day before the marathon. The quadriceps muscle of the thigh and the triceps muscle of the calf were measured. All measurements were performed in a designated room. Rigidity measurements were taken at rest the day before the marathon. Subsequent measurements were made 1–2 h before the start and just after the end of the marathon run. All tests were performed by the same trained person to operate the MYOTON device.
The participants were prone on their backs or their stomachs on a unique bed, and they rested for 10 min before muscle stiffness measurements were taken. Testing sites on each muscle were located using a tape measure and marked using a skin-safe pen (Figure 1 ). A pillow was placed under the head, and a unique roller pillow was placed under the lower leg to aid relaxation. One series of three single Myoton measurements of each muscle group (12 points) were measured separately for the left and right legs. In addition, for a better understanding of the problem, the functionality of the lower limb was also determined - the dominant and non-dominant leg. The dominant leg for a particular runner was determined based on the information provided by the marathoner in the questionnaire.
The reliability between trials (within session) of the one selected muscles (two series of 10 single measurements) of each group was tested using intraclass correlation coefficient (ICC) model. Domholdt (1993) classification scales for interpreting ICCs was used: very high =1.00–0.90; high = 0.89–0.70; moderate = 0.69–0.50; low = 0.49–0.26. This indicated that Rectus femoris reach (ICC = 0.82) and Gastrocnemius (ICC = 0.85). The high reliability coefficient indicated that applied tests represent consistent measurement of muscle stiffness data among the runners.
The participants were prone on their backs or their stomachs on a unique bed, and they rested for 10 min before muscle stiffness measurements were taken. Testing sites on each muscle were located using a tape measure and marked using a skin-safe pen (
The reliability between trials (within session) of the one selected muscles (two series of 10 single measurements) of each group was tested using intraclass correlation coefficient (ICC) model. Domholdt (1993) classification scales for interpreting ICCs was used: very high =1.00–0.90; high = 0.89–0.70; moderate = 0.69–0.50; low = 0.49–0.26. This indicated that Rectus femoris reach (ICC = 0.82) and Gastrocnemius (ICC = 0.85). The high reliability coefficient indicated that applied tests represent consistent measurement of muscle stiffness data among the runners.
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Head
inecalcitol
Lower Extremity
Marathon composite resin
Medical Devices
Muscle, Gastrocnemius
Muscle Rigidity
Muscle Tissue
Quadriceps Femoris
Rectus Femoris
Skin
Stomach
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C57BL/6 mice are a widely used inbred mouse strain commonly used in biomedical research. They are known for their black coat color and are a popular model organism due to their well-characterized genetic and physiological traits.
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More about "Quadriceps Femoris"
The Quadriceps Femoris, also known as the quadriceps or quads, is a powerful muscle group located in the anterior thigh.
Composed of four distinct muscle bellies - the rectus femoris, vastus lateralis, vastus medialis, and vastus intermedius - this group plays a crucial role in knee extension and hip flexion, essential for ambulation and functional movement.
Researchers exploring the Quadriceps Femoris can benefit from the advanced tools offered by PubCompare.ai's AI-driven platform.
This innovative solution allows scientists to effortlessly locate the best experimental protocols from a vast pool of literature, preprints, and patents, ensuring they identify the most accurate and reproducible methods to enhance their research outcomes.
With the platform's comparison tools, researchers can optimize their studies and unlock new insights into this important muscle group.
For example, they can leverage techniques such as TRIzol extraction, RNeasy Mini Kit purification, and high-capacity cDNA reverse transcription to analyze gene expression patterns.
Biodex System 3 can be used for precise measurements of muscle function, while MATLAB can aid in data analysis and visualization.
Furthermore, researchers may incorporate the use of C57BL/6 mice, a widely used model in biomedical research, to investigate Quadriceps Femoris-related physiological and pathological processes.
Complementary techniques like the Pierce BCA Protein Assay and the DC Protein Assay can be employed to quantify protein levels in muscle tissue samples.
By harnessing the power of PubCompare.ai's platform and leveraging these advanced techniques, scientists can optimize their Quadriceps Femoris research, leading to more accurate and reproducible findings that contribute to our understanding of this critical muscle group and its role in human movement and function.
Composed of four distinct muscle bellies - the rectus femoris, vastus lateralis, vastus medialis, and vastus intermedius - this group plays a crucial role in knee extension and hip flexion, essential for ambulation and functional movement.
Researchers exploring the Quadriceps Femoris can benefit from the advanced tools offered by PubCompare.ai's AI-driven platform.
This innovative solution allows scientists to effortlessly locate the best experimental protocols from a vast pool of literature, preprints, and patents, ensuring they identify the most accurate and reproducible methods to enhance their research outcomes.
With the platform's comparison tools, researchers can optimize their studies and unlock new insights into this important muscle group.
For example, they can leverage techniques such as TRIzol extraction, RNeasy Mini Kit purification, and high-capacity cDNA reverse transcription to analyze gene expression patterns.
Biodex System 3 can be used for precise measurements of muscle function, while MATLAB can aid in data analysis and visualization.
Furthermore, researchers may incorporate the use of C57BL/6 mice, a widely used model in biomedical research, to investigate Quadriceps Femoris-related physiological and pathological processes.
Complementary techniques like the Pierce BCA Protein Assay and the DC Protein Assay can be employed to quantify protein levels in muscle tissue samples.
By harnessing the power of PubCompare.ai's platform and leveraging these advanced techniques, scientists can optimize their Quadriceps Femoris research, leading to more accurate and reproducible findings that contribute to our understanding of this critical muscle group and its role in human movement and function.