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> Anatomy > Body Part > Saphenous Vein

Saphenous Vein

The saphenous vein is a prominent superficial vein in the leg that runs from the foot to the groin.
It is commonly used in cardiovascular and other medical procedures, such as coronary artery bypass grafting.
PubCompare.ai's AI-driven platform can help researchers enhance the reproducibility and accuracy of saphenous vein analysis by locating the best protocols and products from literature, preprints, and pattents with ease.
Its AI-powered comparisons identify the optimal solutions for your research needs, improving the quality and efficiency of your saphenous vein studies.

Most cited protocols related to «Saphenous Vein»

1
Modeling Castration-Resistant Prostate Cancer

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Publication 2012
Animals bicalutamide BLOOD Cells Fingers Males Mice, Inbred NOD Mus Neoplasms Operative Surgical Procedures Orchiectomy Recurrence Saphenous Vein SCID Mice Serum
2
Isolation and Expansion of CD34+ Endothelial Progenitor Cells
Venous samples were minced and digested with Liberase 2 (Roche, Basel, Switzerland). For culture selection, 2×105 cells/mL were plated in the presence of Human Medium (HM; complete NeuroCult medium, StemCell Technologies Inc, Vancouver, British Columbia, Canada) that contained basic fibroblast growth factor (10 ng/mL) and epidermal growth factor (20 ng/mL; both from R&D Systems, Minneapolis, Minn) on uncoated wells (n=5).
CD34pos/CD31-negative (CD31neg) cells were isolated from saphenous vein digests either by magnetic bead–assisted cell sorting (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany; n=6) or by fluorescence-activated cell sorting (n 2). Fluorescence-activated cell sorting was performed with a high-speed cell sorter (MoFlo, Beckman Coulter, Fullerton, Colo). Purity of the preparations was assessed by flow cytometry analysis. Differentiation/expansion was performed by seeding CD34posCD31neg cells obtained with each method on fibronectin-coated plates in endothelial growth medium (EGM2, Lonza, Basel, Switzerland) that contained 2% fetal bovine serum (Lonza).
Campagnolo P., Cesselli D., Zen A.A., Beltrami A.P., Kränkel N., Katare R., Angelini G., Emanueli C, & Madeddu P. (2010). Human Adult Vena Saphena Contains Perivascular Progenitor Cells Endowed With Clonogenic and Proangiogenic Potential. Circulation, 121(15), 1735-1745.
Publication 2010
Cells Endothelium Epidermal growth factor Epiphyseal Cartilage Fetal Bovine Serum Fibroblast Growth Factor 2 Fibronectins Flow Cytometry Homo sapiens Liberase Saphenous Vein Stem Cells Veins
3
Evaluation of Phlebotomy Techniques in Rats
To compare the volume and quality of samples obtained by various phlebotomy techniques, we collected blood from eight male rats using first saphenous, then retro-orbital and finally terminal intracardiac bleeding. These rats were not irradiated or treated by any toxic agent. We attempted to obtain 1 ml of blood by each method.
One BRC staff member performed the saphenous bleeds, a procedure with which she had experience. Each unanesthetized rat was manually restrained in a conical plastic bag, and the fur was plucked from its lower leg and thigh. The leg was wiped with ethanol and the saphenous vein was punctured with a 23-gauge needle. The vein was massaged and blood collected into a 2-ml conical tube. ROB was performed as described above by the same experienced Radiation Biology technician who had done most of the ROB procedures that were evaluated in our retrospective analysis. The BRC veterinary staff performed the cardiac bleeds. Each rat was anesthetized with isoflurane and euthanized by cervical dislocation. Its thorax was then opened and blood was taken directly from the heart with a 3-ml syringe and a 22-gauge needle.
We recorded the sample volume obtained using each procedure and tested all the blood samples for alteration by hemolysis, which we assessed using the serum potassium level3 (link), and for blood urea nitrogen (BUN) concentration, because this is a major endpoint for renal injury. Samples were sent to the Clinical Pathology laboratories of the Marshfield Clinic (Marshfield, WI) for testing.
Sharma A., Fish B.L., Moulder J.E., Medhora M., Baker J.E., Mader M, & Cohen E.P. (2014). Safety and blood sample volume and quality of a refined retro-orbital bleeding technique in rats using a lateral approach. Lab animal, 43(2), 63-66.
Publication 2014
BLOOD Chest Clinical Laboratory Services Ethanol Heart Hemolysis Hemorrhage Injuries Isoflurane Joint Dislocations Kidney Males Neck Needles Phlebotomy Potassium Retinal Cone Saphenous Vein Serum Syringes Thigh Urea Nitrogen, Blood Veins
4
Vascular Smooth Muscle Contractility Assay

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Publication 2010
agonists Bath Blood Vessel Buffers Connective Tissue Endothelium Forceps Glucose Ion, Bicarbonate Muscle Contraction Muscle Tissue Natural Springs Norepinephrine Phenobarbital physiology Potassium Saphenous Vein Smooth Muscles Sodium Chloride Sulfate, Magnesium Tissue, Membrane Tissues Transducers
5
Zika virus inoculation in vivo
Zika virus (strain Zika virus/H.sapiens-tc/BRA/2015/Brazil_SPH2015; genbank accession number KU321639.1) isolated from the plasma of a human in Brazil in 2015 was passaged once on Vero cells and then titrated by Vero cell plaque assay. The inoculum was adjusted to 5.0 log10 PFU (corresponding to 7.8 log10 RNA) in 1 ml of RPMI-1640 medium and injected intravenously in the saphenous vein. Inocula were back-titrated by plaque assay to verify the administered dose. The inoculum tested negative for mycoplasma contamination by deep sequencing (not shown).
Coffey L.L., Pesavento P.A., Keesler R.I., Singapuri A., Watanabe J., Watanabe R., Yee J., Bliss-Moreau E., Cruzen C., Christe K.L., Reader J.R., von Morgenland W., Gibbons A.M., Allen A.M., Linnen J., Gao K., Delwart E., Simmons G., Stone M., Lanteri M., Bakkour S., Busch M., Morrison J, & Van Rompay K.K. (2017). Zika Virus Tissue and Blood Compartmentalization in Acute Infection of Rhesus Macaques. PLoS ONE, 12(1), e0171148.
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Publication 2017
Biological Assay Dental Plaque Homo sapiens Mycoplasma Plasma Saphenous Vein Strains Vero Cells Zika Virus

Most recents protocols related to «Saphenous Vein»

1
SARS-CoV-2 Challenge in Transgenic Mice
8–10-week-old BALB/c female mice (Charles River) were immunised with DNA or MVA constructs bearing the gene of interest, and serial bleeds were taken from the saphenous vein. Terminal bleeds were taken via cardiac puncture under isofluorane anaesthesia. For challenge studies, animals were transduced with 1x107 PFU of the ad5-huACE-2 vector in a volume of 75ul by intranasal route (University of Iowa, Viral Vector Core) five days before infection with SARS-CoV-2. Mice were then moved to hermetically sealed isocages at containment level 3 and administered 1x104 PFU of Australia/VIC01/2020 (SARS-CoV-2) by intranasal route under light isofluorane anaesthesia, in a total volume of 40 µl PBS. Animals were weighed and checked twice daily for clinical symptoms and culled on days 3 and 6 post infection by terminal bleed under non recovery anaesthesia. All animal work was approved by the Home Office under project licence P8143424B and approved by the Animal Welfare Ethical Review Body (AWERB). Animal experiments were performed in early 2020 when K18-huACE2 mice colonies were being expanded and were not available.
Carnell G.W., Billmeier M., Vishwanath S., Suau Sans M., Wein H., George C.L., Neckermann P., Del Rosario J.M., Sampson A.T., Einhauser S., Aguinam E.T., Ferrari M., Tonks P., Nadesalingam A., Schütz A., Huang C.Q., Wells D.A., Paloniemi M., Jordan I., Cantoni D., Peterhoff D., Asbach B., Sandig V., Temperton N., Kinsley R., Wagner R, & Heeney J.L. (2023). Glycan masking of a non-neutralising epitope enhances neutralising antibodies targeting the RBD of SARS-CoV-2 and its variants. Frontiers in Immunology, 14, 1118523.
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Publication 2023
Anesthesia Animals Cloning Vectors Ethical Review Females Genes Heart Human Body Infection Light Mice, House Mice, Inbred BALB C Punctures Rivers Saphenous Vein SARS-CoV-2
2
Surgical Management of Inguinal Lymph Nodes
Patients who received simultaneous resection of primary tumor and inguinal lymph nodes were assigned to the immediate group, while other patients were assigned to the delayed group. No palpable or visibly enlarged inguinal lymph nodes when they received prophylactic surgical treatment. The cases were treated in accordance with modern treatment protocol, including standard preoperative imaging, primary tumor treatment, and standard surgical templates. Nodal staging was accomplished by physical examination and imaging. Inguinal computed tomography (CT) examination was used in obese patients in whom palpation was unreliable to exclude lymph nodes enlargement. To other patients, a physical examination of both groins was performed in order to record the number, laterality, and characteristics of inguinal nodes. If nodes were not palpable, inguinal B-ultrasound was performed first.
All these following boundaries constituted the extent of modified ILND: the spermatic cord formed the upper boundary, and the fossa ovalis formed the lower boundary; the inner and outer boundaries were the lateralis of the long adductor muscle and the femoral artery, respectively. Compared with radical ILND, the modified procedure decreased the length of the skin incision and the scope of dissection, preserved the saphenous vein and fascia lata, and avoided the transposition of the sartorius muscle, decreasing morbidity related to groin dissection (14 (link)–16 (link)).
Before 2015 all procedures were open ILNDs, and since 2015 we have performed video-endoscopic ILND. Additional ipsilateral pelvic LND (pLND) (external iliac and obturator) was performed if two or more inguinal lymph nodes were involved after ILND and adjuvant chemotherapy was provided. The Clavien-Dindo classification system was used to judge operation-related complications.
Ma S., Zhao J., Liu Z., Wu T., Wang S., Wu C., Pan L., Jiang X., Guan Z., Wang Y., Jiao D., Yan F., Zhang K., Tang Q, & Ma J. (2023). Prophylactic inguinal lymphadenectomy for high-risk cN0 penile cancer: The optimal surgical timing. Frontiers in Oncology, 13, 1069284.
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Publication 2023
Chemotherapy, Adjuvant Dissection Endoscopy Femoral Artery Functional Laterality Groin Ilium Lata, Fascia Lymphadenopathy Muscle Tissue Neoplasms Nodes, Lymph Obesity Palpation Patients Pelvis Physical Examination Prophylactic Surgical Procedures Saphenous Vein Skin Spermatic Cord Treatment Protocols Ultrasonics X-Ray Computed Tomography
3
Diabetic Mouse Glucose Monitoring
All experiments were carried out in accordance with the UBC Animal Care Committee and Canadian Council on Animal Care. Male non-diabetic 8–14 week old NSG mice (005557 NOD.Cg-Prkdc Il2rg/SzJ) were maintained on a 12-h light/dark cycle with ad libitum access to irradiated standard chow diet (#2919) and regular xGVHD monitoring19 . Blood glucose was measured in saphenous vein samples using a OneTouch Verio glucometer (LifeScan) or in intraperitoneal glucose-stimulated insulin secretion tests (ipGSIS), in which mice were fasted for 6 hours then challenged with 2 g/kg glucose. Human C-peptide and engrafted cell phenotype were measured weekly.
Ellis C.E., Mojibian M., Ida S., Fung V.C., Skovsø S., McIver E., O’Dwyer S., Webber T.D., Braam M.J., Saber N., Kieffer T.J, & Levings M.K. (2023). Human A2-CAR T cells reject HLA-A2+ human islets transplanted into mice without inducing graft versus host disease. bioRxiv.
Publication Preprint 2023
Animal Care Committees Animals Blood Glucose C-Peptide Cells Glucose Homo sapiens IL2RG protein, human Insulin Secretion Males Mice, Laboratory Phenotype PRKDC protein, human Saphenous Vein Therapy, Diet
4
Chest PET-CT Imaging of Antigen Recall
Chest PET-CT was performed at baseline prior to antigen recall (D0) and on days 3 (D3) and 7 (D7) post recall. Animals were fasted for at least 8 h before each imaging session. All imaging acquisition was performed using the Digital Photon Counting (DPC) PET-CT system (Vereos-Ingenuity, Philips) implemented in a BSL-3 laboratory. These sessions were always performed in the same experimental conditions (acquisition time and animal order) to limit [18F]-FDG-PET experimental bias. Animals were first anesthetized with ketamine hydrochloride (5 mg/kg, IM) associated with medetomidine hydrochloride (0.05mg/kg IM), intubated, and then maintained under 0.5–1.5% isoflurane and placed in a supine position on a warming blanket (Bear Hugger, 3M) on the machine bed with monitoring of the cardiac rate, oxygen saturation, and body temperature.
Computed Tomography (CT) was performed 5 minutes prior to PET acquisition for attenuation correction and anatomical localization. The CT detector collimation used was 64 × 0.6 mm, the tube voltage was 120 kV, and the intensity was approximately 150 mAs. Chest-CT images were reconstructed with a slice thickness of 1.25 mm and an interval of 0.63 mm. A whole-body PET scan (3 bed positions, 3 min/bed position) was performed approximately 45 min post-injection of 3.5 ± 0.4 MBq kg-1 of [18F]-FDG via the saphenous vein. PET images were reconstructed onto a 256 × 256 matrix using OSEM (3 iterations, 15 subsets). After PET acquisition, animals were resuscitate using atipamezole hydrochloride (0.25mg/kg).
PET images were analyzed using 3DSlicer software (open-source tool). For segmentation, various regions of interest (entire lung and separated lung lobes) were semi-automatically contoured according to anatomical information from CT. A 3D volume of interest (VOI) was interpolated from several ROIs in different image slices to cover each lung lobe excluding background signal (heart and liver). For quantification, [18F]-FDG accumulation in the VOIs was given as a standardized uptake value (SUVmean, SUVmax).
Woodworth J.S., Contreras V., Christensen D., Naninck T., Kahlaoui N., Gallouët A.S., Langlois S., Burban E., Joly C., Gros W., Dereuddre-Bosquet N., Morin J., Olsen M.L., Rosenkrands I., Stein A.K., Wood G.K., Follmann F., Lindenstrøm T., LeGrand R., Pedersen G.K, & Mortensen R. (2023). A novel adjuvant formulation induces robust Th1/Th17 memory and mucosal recall responses in Non-Human Primates. bioRxiv.
Publication Preprint 2023
Animals Antigens atipamezole Bears Body Temperature Chest F18, Fluorodeoxyglucose Heart Isoflurane Ketamine Hydrochloride Liver Lung Medetomidine Hydrochloride Mental Recall Oxygen Saturation Rate, Heart Saphenous Vein Whole Body Imaging X-Ray Computed Tomography
5
Serum Collection in ADPKD Monkeys
Before the ADPKD monkeys died, we collected serum by venipuncture of the saphenous vein using a 5-mm animal prick needle (Goldenrod Animal Lancet, Braintree Scientific). Then, 2 ml of blood was collected in an anticoagulant-free blood collection tube (BD vacutainer, SST II Advance). The monkeys were carefully monitored for 30 min following blood withdrawal to watch for signs of distress. An additional 0.5 ml of blood was collected into an EDTA-K2 tube. Biochemical index levels were determined by Kunming Biomed International Laboratory using a Roche Cobas C501 (Roche, Germany). Hemocytometry was performed on a Sysmex XT-2000i (Sysmex, Japan).
Wu R., Bai B., Li F., Bai R., Zhuo Y., Zhu Z., Jia R., Li S., Chen Y, & Lan X. (2023). Phenotypes and genetic etiology of spontaneous polycystic kidney and liver disease in cynomolgus monkey. Frontiers in Veterinary Science, 10, 1106016.
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Publication 2023
Animals Anticoagulants BLOOD Edetic Acid golden rod extract Monkeys Needles Phlebotomy Polycystic Kidney, Autosomal Dominant Saphenous Vein Serum

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More about "Saphenous Vein"

The saphenous vein, a prominent superficial vein in the leg, plays a crucial role in various medical procedures, including cardiovascular surgeries like coronary artery bypass grafting.
This vein, which runs from the foot to the groin, is commonly utilized in these procedures.
To enhance the reproducibility and accuracy of saphenous vein analysis, researchers can leverage the power of PubCompare.ai's AI-driven platform.
This innovative solution helps researchers locate the best protocols and products from the literature, preprints, and patents, making it easier to identify the optimal solutions for their research needs.
The saphenous vein is also relevant to other medical devices and products, such as the Accu-Chek Aviva blood glucose monitor, EDTA-coated tubes for blood collection, and BD Vacutainer blood collection systems.
Additionally, diabetes management tools like the OneTouch UltraMini and insulin products like Humulin R and Novolin ge Toronto may be associated with conditions or procedures involving the saphenous vein.
By leveraging the insights and capabilities of PubCompare.ai, researchers can improve the quality and efficiency of their saphenous vein studies, ultimately enhancing the reproducibility and accuracy of their findings.
This comprehensive approach to vein analysis can lead to advancements in cardiovascular and other medical fields, benefiting both researchers and patients.