Synovial tissue was obtained during open joint replacement surgery or arthroscopic synovectomy from a total of 16 patients with the clinical diagnosis of RA (13 knee joints, one hip joint, one wrist, one metacarpo-phalangeal joint) as well as 21 patients with the clinical diagnosis of OA (all knee joints; Table 2 ) from the Department of Orthopedics, University of Leipzig, Germany, the Clinic of Orthopedics, Bad Düben, Germany, and the Clinic of Orthopedics, Eisenberg, Germany, as well as the Department of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA. All RA patients fulfilled the American Rheumatism Association criteria for RA [8 (link)]. The study was approved by the Ethics Committees of the University of Leipzig and the University of Jena, Germany, and the University of Michigan, MI, USA. One portion of each sample was immediately frozen in isopentane (Merck, Darmstadt, Germany), cooled in liquid nitrogen and stored at –70°C for immunohistochemistry. The remaining tissue was placed in cell culture medium at ambient temperature and subjected to tissue digestion within 2 h.
Synovial Membrane
The synovial membrane is a thin, vascular connective tissue that lines the inner surface of joints, tendon sheaths, and bursae.
It serves to reduce friction and facilitate smooth movement within these structures.
The membrane produces synovial fluid, which lubricates and nourishes the cartilage.
Understanding the structure and function of the synovial membrane is crucial for researching joint diseases, such as arthritis, and developing effective therapies.
PubCompare.ai's platform can help streamline your synovial membrane research by providing access to the best protocols and methodolgies from literature, preprints, and patents, enabling you to identify the most accurate and reproducible approaches.
It serves to reduce friction and facilitate smooth movement within these structures.
The membrane produces synovial fluid, which lubricates and nourishes the cartilage.
Understanding the structure and function of the synovial membrane is crucial for researching joint diseases, such as arthritis, and developing effective therapies.
PubCompare.ai's platform can help streamline your synovial membrane research by providing access to the best protocols and methodolgies from literature, preprints, and patents, enabling you to identify the most accurate and reproducible approaches.
Most cited protocols related to «Synovial Membrane»
Arthroplasty, Replacement
Arthroscopy
Bones of Fingers
Cell Culture Techniques
Cells
Collagen Diseases
Culture Media
Diagnosis
Digestion
Ethics Committees
Freezing
Hip Joint
Immunohistochemistry
isopentane
Isotretinoin
Joints
Knee Joint
Nitrogen
Orthopedic Rehabilitation Surgery
Orthopedic Surgical Procedures
Patients
Synovectomy
Synovial Membrane
Tissues
Wrist Joint
The RAAK study is aimed at the biobanking of joint materials as well as mesenchymal stem cells and primary chondrocytes from patients and controls in the Leiden University Medical Center and collaborating outpatient clinics in the Leiden area. In the current study we used paired preserved and OA affected cartilage samples for 33 donors undergoing joint replacement surgery for primary OA (22 hips, 11 knees). Characteristics of the donors are shown in Table S1 .
At the moment of collection (within 2 hours following surgery) tissue was washed extensively with phosphate buffered saline (PBS) to decrease the risk of contamination by blood. Cartilage was classified macroscopically and collected separately from OA affected and preserved regions around the weight-bearing area of the joint (Figure S1 ). Classification was done based on predefined features of OA related damage as described previously [9] (link), [10] (link): color/whiteness of the cartilage, surface integrity as determined by visible fibrillation/crack formation, and depth and hardness of the cartilage upon sampling with a scalpel. Care was taken to avoid contamination with bone or synovium. Collected cartilage was snap frozen in liquid nitrogen and stored at −80°C prior to RNA extraction.
At the moment of collection (within 2 hours following surgery) tissue was washed extensively with phosphate buffered saline (PBS) to decrease the risk of contamination by blood. Cartilage was classified macroscopically and collected separately from OA affected and preserved regions around the weight-bearing area of the joint (
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Arthroplasty, Replacement
BLOOD
Bones
Cartilage
Chondrocyte
Coxa
Donors
Freezing
Joints
Knee
Mesenchymal Stem Cells
Nitrogen
Operative Surgical Procedures
Patients
Phosphates
Saline Solution
Synovial Membrane
Tissues
Synovial membrane samples were obtained either from postmortem joints/traumatic joint injury cases (control group (CG); n = 15 and n = 5, respectively) or from RA/OA patients (all Caucasian) upon joint replacement/synovectomy at the Jena University Hospital, Chair of Orthopedics, Waldkrankenhaus ‘Rudolf Elle’, Eisenberg, Germany (n = 33, dataset ‘Jena’), at the Department of Orthopedics/Institute of Pathology/Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin (n = 30, dataset ‘Berlin’), and at the Department of Orthopedics/Institute of Pathology, University of Leipzig (n = 16, dataset ‘Leipzig’). After removal, tissue samples were frozen and stored at −70°C.
The study was approved by the respective ethics committees (Jena University Hospital: Ethics Committee of the Friedrich Schiller University Jena at the Medical Faculty; Charité-Universitätsmedizin Berlin: Charité Ethics Committee; and University of Leipzig: Ethics Committee at the Medical Faculty of the University of Leipzig) and informed patient consent was obtained. RA patients were classified according to the American College of Rheumatology criteria valid in the sample assessment period [15 (link)], OA patients were classified according to the respective criteria for OA [16 (link)]. The patients/donors were assigned to one of the three terms (categorical values): ‘CG’, ‘RA’, or ‘OA’ (for clinical characteristics of the donors/patients, see Table1 ).
The study was approved by the respective ethics committees (Jena University Hospital: Ethics Committee of the Friedrich Schiller University Jena at the Medical Faculty; Charité-Universitätsmedizin Berlin: Charité Ethics Committee; and University of Leipzig: Ethics Committee at the Medical Faculty of the University of Leipzig) and informed patient consent was obtained. RA patients were classified according to the American College of Rheumatology criteria valid in the sample assessment period [15 (link)], OA patients were classified according to the respective criteria for OA [16 (link)]. The patients/donors were assigned to one of the three terms (categorical values): ‘CG’, ‘RA’, or ‘OA’ (for clinical characteristics of the donors/patients, see Table
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Arthroplasty, Replacement
Autopsy
Caucasoid Races
Donors
Ethics Committees
Faculty, Medical
Freezing
Injuries
Joints
Orthopedic Surgical Procedures
Patients
Synovectomy
Synovial Membrane
Tissues
Synovial tissues were obtained from synovial biopsies of six patients with RA undergoing joint surgery (synovectomy or joint replacement by prosthesis implantation), who all met the criteria of the American College of Rheumatology [27 (link)]. The tissue samples were obtained during routine surgery at the Department of Orthopedics of the University of Regensburg, where approved by the local ethics committee and patients involved gave informed consent. Culture of SF was performed as described recently [5 (link)]. Following enzymatic digestion, fibroblasts were grown in DMEM (Biochrom, Berlin, Germany) containing 10% heat inactivated FCS (Gibco Life Technologies, Grand Island, NY, USA), 100 U/ml penicillin and streptomycin (PAA Laboratories GmbH, Linz, Austria) and cultured for four passages at 37°C in 10% carbon dioxide. The SF were stained for a fibroblast marker by immunohistochemistry. More than 95% could be stained positively for the fibroblast enzyme prolyl 4-hydroxylase and none were positive for the macrophage marker CD68 or the neutrophil marker cathepsin G after the second passage of cultivation with enzymatic digestion equalling passage 0 (data not shown). Routine tests for mycoplasms were negative. At 85 to 95% confluency, cells were passaged 1:2 and a part of the cells was harvested. Total RNA was extracted and stored at -70°C. Culture conditions were (and have to be) kept constant during the experiments. Passaging of the cells was performed at 85 to 95% confluency as fibroblasts exhibit contact inhibition (unpublished observations) and were passaged 1:2 to provide cell-cell contacts between the cells.
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Arthroplasty, Replacement
Biopsy
Carbon dioxide
Cathepsin G
Cells
Contact Inhibition
Digestion
Enzymes
Fibroblasts
Immunohistochemistry
Joints
Macrophage
Neutrophil
Operative Surgical Procedures
Orthopedic Surgical Procedures
Patients
Penicillins
Pleuropneumonia-Like Organisms
Procollagen-Proline Dioxygenase
Regional Ethics Committees
Streptomycin
Synovectomy
Synovial Membrane
Tissues
Cells
Centrifugation
Chemotaxis
Collagenase
Countercurrent Chromatography
Deoxyribonucleases
dispase
Donors
Ethics Committees, Research
Fibroblasts
histopaque
IL17A protein, human
Macrophage
Monocytes
Proteins
RNA, Messenger
Synovial Membrane
Tissues
Most recents protocols related to «Synovial Membrane»
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Adult
Arthropathy
Bone-Implant Interface
Connective Tissue Diseases
Diagnosis
Ethanol
Ethics Committees, Clinical
Females
Femoral Fractures
Femur Heads
Fever
Fracture, Bone
Head
Homo sapiens
Infection
Joints
Males
Necrosis
Pain
Paraffin Embedding
paraform
Patient Participation
Patients
Repeat Surgery
Sinuses, Nasal
Synovial Fluid
Synovial Membrane
System, Immune
Tissue, Membrane
Tissues
Total Hip Arthroplasty
X-Rays, Diagnostic
We downloaded the GSE55235 (26 (link)), GSE55457 (26 (link)), and GSE77298 (25 (link)) RA datasets from the Gene Expression Omnibusdatabase (https://www.ncbi.nlm.nih.gov/geo/ ) using RStudio software (version 4.0.2; URL: https://www.r-project.org/ ). All dataset processing and analysis were performed in RStudio. The GSE55235 dataset (GPL96 platform), which was uploaded in 2014, contains transcriptome analyses of synovial tissue from 10 RA patients and 8 individuals with healthy joints. The GSE55457 dataset (GPL96 platform) identified 13 synovial membrane samples from patients with RA and 10 normal control synovial membrane samples. The samples in GSE55235, GSE55457 datasets were obtained from patients with RA for more than ten years. The RA dataset GSE77298 (GPL570 platform) contained a total of 23 synovial samples, which were obtained from 16 RA patients and 7 healthy individuals. In this dataset, the synovial samples were obtained from early RA at the Department of Rheumatic. Depression-associated transcriptomes (GSE98793) were obtained from the GEO database. In the GSE98793 dataset (27 (link)) (GPL570 platform), whole blood from 128 patients with MDD samples and 64 healthy individuals was collected. MDD was defined as CORE score >=8. According to the different sources of the samples, we categorized the samples into the RA group, depression group, and normal group, respectively. A simplified workflow of the current investigation is presented in Figure 1 .
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BLOOD
Gene Expression
Gene Expression Profiling
Joints
Patients
Synovial Membrane
Transcriptome
The technique follows the general steps of the original technique described by Guimberteau9 (link) with modifications that avoid division of the ulnar artery, making it more attractive to hand surgeons. Through a Bruner incision, the remnants of the FPL are resected from the digital canal in the thumb, preserving the oblique pulley if present, and otherwise reconstructing the annular pulley. The FDS of the fourth finger is exposed from the distal forearm to the proximal interphalangeal joint level through a zigzag incision. The branch of the ulnar artery to the common carpal synovial sheath is identified and preserved (Fig. 1 ). The tendon and its investing synovial tissue are elevated based on this vascular branch. The FDS IV tendon is cut proximally at the myotendinous junction and distally at the proximal interphalangeal joint level. Care is taken not to disrupt the synovial tissue around the tendon. Injuries of the synovial sheath are sutured with 8/0 nylon. A sublimus sling is performed in the donor fourth finger to avoid a swan-neck deformity. The pedicled tendon flap is transferred to the thumb, deep to the median nerve, without dividing the ulnar artery (Fig. 2 ). The distal end of the FDS is pulled through the digital canal with a tendon passer. Care is taken not to strip the synovial envelope of the tendon when passing under the oblique pulley. If an annular pulley is reconstructed, the tension is adjusted to avoid overconstriction of the vascularity of the synovial sheath. Distal fixation is performed with a pull-out transosseous suture to the P2 and a proximal Pulvertaft repair is performed to the FPL at the distal forearm. Full flexion of the thumb with the wrist in neutral is needed to avoid undue tension on the vascular pedicle. If tension is a concern, the ulnar vascular bundle can be separated from the ulnar nerve and mobilized radially. The deep palmar branch of the ulnar artery does not need to be ligated. Enough mobilization is possible through ligation of more proximal minor branches. The volar carpal retinaculum is reconstructed with a retinacular flap (Fig. 3 ).
The hand is immobilized in a dorsal splint with the wrist in neutral position, the thumb metacarpal adducted, and the metacarpophalanx joint fully flexed. Passive range of movement exercises are started on postoperative day two. Protected active range of movement exercises are started postoperatively at week five and continued until week 12. After the 12th week, full active flexion is allowed (Fig.4 ).
The hand is immobilized in a dorsal splint with the wrist in neutral position, the thumb metacarpal adducted, and the metacarpophalanx joint fully flexed. Passive range of movement exercises are started on postoperative day two. Protected active range of movement exercises are started postoperatively at week five and continued until week 12. After the 12th week, full active flexion is allowed (Fig.
Arecaceae
Arteries, Ulnar
Blood Vessel
Bones, Metacarpal
Congenital Abnormality
Forearm
Injuries
Joints
Ligation
Movement
Myotendinous Junction
Neck
Nerves, Median
Nylons
Passive Range of Motion
Pedicled Flap
Pulp Canals
Splints
Surgeons
Surgical Flaps
Sutures
Synovial Membrane
Tendons
Thumb
Tissue Donors
Ulnar Nerve
Wrist
Wrist Joint
We retrospectively analyzed patients with metastatic STS treated with antiangiogenic agents plus PD-1 inhibitors in Henan Cancer Hospital, the Fifth Affiliated Hospital of Zhengzhou University, and Henan Provincial People’s Hospital between June 2019 and May 2022. This study complied with the principles of Helsinki, met the requirements of the ethics committee and was approved by the ethics committees of each institute. All participants provided written informed consent before treatment.
Patients were included according to the main criteria: 1. Age 18 to 70; 2. The performance status of Eastern Tumor Cooperative Group (ECOG) is 0-2; 3. The pathological diagnosis included ASPS, UPS, synovium, LMS, epithelioid sarcoma (ES), fibrosarcoma, etc. 4. At least one measure based on the Response Evaluation Criteria for Solid Tumors (RECIST) 1.1; 5. Complete medical history and follow-up records; 6. Not eligible for or refusing first-line chemotherapy; and 7. Progressive disease within 6 months before combination treatment. Patients were excluded if they presented contraindications of antiangiogenic agents and/or PD-1 inhibitors including coagulation dysfunction, active asthma, etc.
Patients were included according to the main criteria: 1. Age 18 to 70; 2. The performance status of Eastern Tumor Cooperative Group (ECOG) is 0-2; 3. The pathological diagnosis included ASPS, UPS, synovium, LMS, epithelioid sarcoma (ES), fibrosarcoma, etc. 4. At least one measure based on the Response Evaluation Criteria for Solid Tumors (RECIST) 1.1; 5. Complete medical history and follow-up records; 6. Not eligible for or refusing first-line chemotherapy; and 7. Progressive disease within 6 months before combination treatment. Patients were excluded if they presented contraindications of antiangiogenic agents and/or PD-1 inhibitors including coagulation dysfunction, active asthma, etc.
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4-maleimido-2,2,6,6-tetramethylpiperidinooxyl
Angiogenesis Inhibitors
ASIP protein, human
Asthma
Coagulation, Blood
Diagnosis
Electrocorticography
Ethics Committees
Fibrosarcoma
Neoplasms
Patients
Pharmacotherapy
Programmed Cell Death Protein 1 Inhibitor
Sarcoma, Epithelioid
Synovial Membrane
Synovial samples were obtained intra-operatively from 147 OA patients and 60 RA patients. As per the study protocol, orthopedic surgeons were requested to preferentially obtain a research sample from grossly abnormal-looking synovium. Tissue for histological examination was chosen by a pathologist on the basis of gross features including the smoothness and granularity of the synovial surface, red or brown discoloration, and the clarity, dullness, or opacity of the synovial layer, preferentially avoiding regions of electro-cautery effect.
Synovial samples were preferentially obtained from the most grossly inflamed (dull and opaque) area of the synovium. If there was no obviously inflamed synovium, samples were obtained from standard locations: the femoral aspects of the medial and lateral gutters and the central supratrochlear region of the suprapatellar pouch. OA synovial tissue samples were formalin-fixed and paraffin-embedded, and the RA tissues were fresh-frozen in optimal cutting temperature compound. Each tissue biopsy was sectioned at 5-μm thickness and stained with Harris-modified hematoxylin solution and eosin Y (H&E) manufactured by Epredia in Kalamazoo, MI. An expert musculoskeletal pathologist (ED) scored fourteen synovial histologic features in a single section for each patient: lymphocytic inflammation, mucoid change, fibrosis, fibrin, germinal centers, lining hyperplasia, neutrophils, detritus, plasma cells, binucleated plasma cells, Russell bodies, sub-lining giant cells, synovial lining giant cells, and mast cells. Detailed methods for scoring these features are included in theAppendix , some of which are described in prior studies [8 ] and available at www.hss.edu/pathology-synovitis .
Synovial samples were preferentially obtained from the most grossly inflamed (dull and opaque) area of the synovium. If there was no obviously inflamed synovium, samples were obtained from standard locations: the femoral aspects of the medial and lateral gutters and the central supratrochlear region of the suprapatellar pouch. OA synovial tissue samples were formalin-fixed and paraffin-embedded, and the RA tissues were fresh-frozen in optimal cutting temperature compound. Each tissue biopsy was sectioned at 5-μm thickness and stained with Harris-modified hematoxylin solution and eosin Y (H&E) manufactured by Epredia in Kalamazoo, MI. An expert musculoskeletal pathologist (ED) scored fourteen synovial histologic features in a single section for each patient: lymphocytic inflammation, mucoid change, fibrosis, fibrin, germinal centers, lining hyperplasia, neutrophils, detritus, plasma cells, binucleated plasma cells, Russell bodies, sub-lining giant cells, synovial lining giant cells, and mast cells. Detailed methods for scoring these features are included in the
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Biopsy
Cauterization
Cytoplasmic Granules
Eosin
Femur
Fibrin
Fibrosis
Formalin
Freezing
Germinal Center
Giant Cells
Human Body
Hyperplasia
Inflammation
Lymphocyte
Mast Cell
Neutrophil
Orthopedic Surgeons
Paraffin
Pathologists
Patients
Plasma Cells
Synovial Membrane
Synovitis
Tissues
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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Collagenase is an enzyme that breaks down collagen, the primary structural protein found in the extracellular matrix of various tissues. It is commonly used in cell isolation and tissue dissociation procedures.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Collagenase type II is a purified enzyme derived from Clostridium histolyticum. It is used for the dissociation of a variety of cell types, particularly those with a high collagen content, such as cartilage and connective tissue.
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Collagenase type I is an enzyme used in laboratory settings to break down collagen, a structural protein found in various tissues. It is commonly used in cell isolation and tissue dissociation procedures.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
More about "Synovial Membrane"
The synovial membrane, also known as the synovial lining or synovial layer, is a thin, specialized connective tissue that lines the inner surface of joints, tendon sheaths, and bursae.
This highly vascular membrane plays a crucial role in reducing friction and facilitating smooth movement within these structures.
The synovial membrane is responsible for producing synovial fluid, a lubricating and nourishing liquid that helps maintain the health of the surrounding cartilage.
Understanding the structure and function of the synovial membrane is essential for researching joint diseases, such as osteoarthritis and rheumatoid arthritis, and developing effective therapies.
Researchers often utilize various cell culture and tissue engineering techniques to study the synovial membrane, including the use of cell culture media like Fetal Bovine Serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM), as well as reagents like TRIzol for RNA extraction and Collagenase for tissue digestion.
To streamline your synovial membrane research, PubCompare.ai's advanced AI-driven platform can provide you with access to the best protocols and methodologies from literature, preprints, and patents.
By leveraging AI-powered comparisons, you can identify the most accurate and reproducible approaches, helping you drive meaningful insights and accelerate your research progress.
Whether you're studying the synovial membrane's role in joint health or exploring new therapeutic interventions, PubCompare.ai's tools can be invaluable in your research journey.
This highly vascular membrane plays a crucial role in reducing friction and facilitating smooth movement within these structures.
The synovial membrane is responsible for producing synovial fluid, a lubricating and nourishing liquid that helps maintain the health of the surrounding cartilage.
Understanding the structure and function of the synovial membrane is essential for researching joint diseases, such as osteoarthritis and rheumatoid arthritis, and developing effective therapies.
Researchers often utilize various cell culture and tissue engineering techniques to study the synovial membrane, including the use of cell culture media like Fetal Bovine Serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM), as well as reagents like TRIzol for RNA extraction and Collagenase for tissue digestion.
To streamline your synovial membrane research, PubCompare.ai's advanced AI-driven platform can provide you with access to the best protocols and methodologies from literature, preprints, and patents.
By leveraging AI-powered comparisons, you can identify the most accurate and reproducible approaches, helping you drive meaningful insights and accelerate your research progress.
Whether you're studying the synovial membrane's role in joint health or exploring new therapeutic interventions, PubCompare.ai's tools can be invaluable in your research journey.