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Synovial Membrane

The synovial membrane is a thin, vascular connective tissue that lines the inner surface of joints, tendon sheaths, and bursae.
It serves to reduce friction and facilitate smooth movement within these structures.
The membrane produces synovial fluid, which lubricates and nourishes the cartilage.
Understanding the structure and function of the synovial membrane is crucial for researching joint diseases, such as arthritis, and developing effective therapies.
PubCompare.ai's platform can help streamline your synovial membrane research by providing access to the best protocols and methodolgies from literature, preprints, and patents, enabling you to identify the most accurate and reproducible approaches.

Most cited protocols related to «Synovial Membrane»

Synovial tissue was obtained during open joint replacement surgery or arthroscopic synovectomy from a total of 16 patients with the clinical diagnosis of RA (13 knee joints, one hip joint, one wrist, one metacarpo-phalangeal joint) as well as 21 patients with the clinical diagnosis of OA (all knee joints; Table 2) from the Department of Orthopedics, University of Leipzig, Germany, the Clinic of Orthopedics, Bad Düben, Germany, and the Clinic of Orthopedics, Eisenberg, Germany, as well as the Department of Orthopedic Surgery, University of Michigan, Ann Arbor, MI, USA. All RA patients fulfilled the American Rheumatism Association criteria for RA [8 (link)]. The study was approved by the Ethics Committees of the University of Leipzig and the University of Jena, Germany, and the University of Michigan, MI, USA. One portion of each sample was immediately frozen in isopentane (Merck, Darmstadt, Germany), cooled in liquid nitrogen and stored at –70°C for immunohistochemistry. The remaining tissue was placed in cell culture medium at ambient temperature and subjected to tissue digestion within 2 h.
Publication 2000
Arthroplasty, Replacement Arthroscopy Bones of Fingers Cell Culture Techniques Cells Collagen Diseases Culture Media Diagnosis Digestion Ethics Committees Freezing Hip Joint Immunohistochemistry isopentane Isotretinoin Joints Knee Joint Nitrogen Orthopedic Rehabilitation Surgery Orthopedic Surgical Procedures Patients Synovectomy Synovial Membrane Tissues Wrist Joint
The RAAK study is aimed at the biobanking of joint materials as well as mesenchymal stem cells and primary chondrocytes from patients and controls in the Leiden University Medical Center and collaborating outpatient clinics in the Leiden area. In the current study we used paired preserved and OA affected cartilage samples for 33 donors undergoing joint replacement surgery for primary OA (22 hips, 11 knees). Characteristics of the donors are shown in Table S1.
At the moment of collection (within 2 hours following surgery) tissue was washed extensively with phosphate buffered saline (PBS) to decrease the risk of contamination by blood. Cartilage was classified macroscopically and collected separately from OA affected and preserved regions around the weight-bearing area of the joint (Figure S1). Classification was done based on predefined features of OA related damage as described previously [9] (link), [10] (link): color/whiteness of the cartilage, surface integrity as determined by visible fibrillation/crack formation, and depth and hardness of the cartilage upon sampling with a scalpel. Care was taken to avoid contamination with bone or synovium. Collected cartilage was snap frozen in liquid nitrogen and stored at −80°C prior to RNA extraction.
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Publication 2014
Arthroplasty, Replacement BLOOD Bones Cartilage Chondrocyte Coxa Donors Freezing Joints Knee Mesenchymal Stem Cells Nitrogen Operative Surgical Procedures Patients Phosphates Saline Solution Synovial Membrane Tissues
Synovial membrane samples were obtained either from postmortem joints/traumatic joint injury cases (control group (CG); n = 15 and n = 5, respectively) or from RA/OA patients (all Caucasian) upon joint replacement/synovectomy at the Jena University Hospital, Chair of Orthopedics, Waldkrankenhaus ‘Rudolf Elle’, Eisenberg, Germany (n = 33, dataset ‘Jena’), at the Department of Orthopedics/Institute of Pathology/Department of Rheumatology and Clinical Immunology, Charité-Universitätsmedizin Berlin (n = 30, dataset ‘Berlin’), and at the Department of Orthopedics/Institute of Pathology, University of Leipzig (n = 16, dataset ‘Leipzig’). After removal, tissue samples were frozen and stored at −70°C.
The study was approved by the respective ethics committees (Jena University Hospital: Ethics Committee of the Friedrich Schiller University Jena at the Medical Faculty; Charité-Universitätsmedizin Berlin: Charité Ethics Committee; and University of Leipzig: Ethics Committee at the Medical Faculty of the University of Leipzig) and informed patient consent was obtained. RA patients were classified according to the American College of Rheumatology criteria valid in the sample assessment period [15 (link)], OA patients were classified according to the respective criteria for OA [16 (link)]. The patients/donors were assigned to one of the three terms (categorical values): ‘CG’, ‘RA’, or ‘OA’ (for clinical characteristics of the donors/patients, see Table 1).
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Publication 2014
Arthroplasty, Replacement Autopsy Caucasoid Races Donors Ethics Committees Faculty, Medical Freezing Injuries Joints Orthopedic Surgical Procedures Patients Synovectomy Synovial Membrane Tissues
Synovial tissues were obtained from synovial biopsies of six patients with RA undergoing joint surgery (synovectomy or joint replacement by prosthesis implantation), who all met the criteria of the American College of Rheumatology [27 (link)]. The tissue samples were obtained during routine surgery at the Department of Orthopedics of the University of Regensburg, where approved by the local ethics committee and patients involved gave informed consent. Culture of SF was performed as described recently [5 (link)]. Following enzymatic digestion, fibroblasts were grown in DMEM (Biochrom, Berlin, Germany) containing 10% heat inactivated FCS (Gibco Life Technologies, Grand Island, NY, USA), 100 U/ml penicillin and streptomycin (PAA Laboratories GmbH, Linz, Austria) and cultured for four passages at 37°C in 10% carbon dioxide. The SF were stained for a fibroblast marker by immunohistochemistry. More than 95% could be stained positively for the fibroblast enzyme prolyl 4-hydroxylase and none were positive for the macrophage marker CD68 or the neutrophil marker cathepsin G after the second passage of cultivation with enzymatic digestion equalling passage 0 (data not shown). Routine tests for mycoplasms were negative. At 85 to 95% confluency, cells were passaged 1:2 and a part of the cells was harvested. Total RNA was extracted and stored at -70°C. Culture conditions were (and have to be) kept constant during the experiments. Passaging of the cells was performed at 85 to 95% confluency as fibroblasts exhibit contact inhibition (unpublished observations) and were passaged 1:2 to provide cell-cell contacts between the cells.
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Publication 2010
Arthroplasty, Replacement Biopsy Carbon dioxide Cathepsin G Cells Contact Inhibition Digestion Enzymes Fibroblasts Immunohistochemistry Joints Macrophage Neutrophil Operative Surgical Procedures Orthopedic Surgical Procedures Patients Penicillins Pleuropneumonia-Like Organisms Procollagen-Proline Dioxygenase Regional Ethics Committees Streptomycin Synovectomy Synovial Membrane Tissues
The studies were approved by the Northwestern University Institutional Review Board, and all donors gave informed written consent. RA and normal synovial tissue fibroblasts were isolated from fresh synovial tissues by mincing and digesting in a solutionof dispase, collagenase, and DNase (20 (link), 21 (link)). Cells were used between passages 3–9. RA and normal synovial tissue fibroblasts were treated with IL-17 for 0–8h for mRNA studies and cell supernatants were harvested after 24h for protein studies. Monocytes were separated from buffy coats (Lifesource, Chicago, IL) obtained from healthy donors. Mononuclear cells, isolated by Histopaque (Sigma-Aldrich, St. Louis, MO) gradient centrifugation, were separated by countercurrent centrifugal elutriation. Monocytes were used for chemotaxis or allowed to differentiate to macrophages as previously described (22 (link), 23 (link)). Macrophages were treated for 0–8h for mRNA studies and 24h for protein studies.
Publication 2010
Cells Centrifugation Chemotaxis Collagenase Countercurrent Chromatography Deoxyribonucleases dispase Donors Ethics Committees, Research Fibroblasts histopaque IL17A protein, human Macrophage Monocytes Proteins RNA, Messenger Synovial Membrane Tissues

Most recents protocols related to «Synovial Membrane»

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Publication 2023
Adult Arthropathy Bone-Implant Interface Connective Tissue Diseases Diagnosis Ethanol Ethics Committees, Clinical Females Femoral Fractures Femur Heads Fever Fracture, Bone Head Homo sapiens Infection Joints Males Necrosis Pain Paraffin Embedding paraform Patient Participation Patients Repeat Surgery Sinuses, Nasal Synovial Fluid Synovial Membrane System, Immune Tissue, Membrane Tissues Total Hip Arthroplasty X-Rays, Diagnostic
We downloaded the GSE55235 (26 (link)), GSE55457 (26 (link)), and GSE77298 (25 (link)) RA datasets from the Gene Expression Omnibusdatabase (https://www.ncbi.nlm.nih.gov/geo/) using RStudio software (version 4.0.2; URL: https://www.r-project.org/). All dataset processing and analysis were performed in RStudio. The GSE55235 dataset (GPL96 platform), which was uploaded in 2014, contains transcriptome analyses of synovial tissue from 10 RA patients and 8 individuals with healthy joints. The GSE55457 dataset (GPL96 platform) identified 13 synovial membrane samples from patients with RA and 10 normal control synovial membrane samples. The samples in GSE55235, GSE55457 datasets were obtained from patients with RA for more than ten years. The RA dataset GSE77298 (GPL570 platform) contained a total of 23 synovial samples, which were obtained from 16 RA patients and 7 healthy individuals. In this dataset, the synovial samples were obtained from early RA at the Department of Rheumatic. Depression-associated transcriptomes (GSE98793) were obtained from the GEO database. In the GSE98793 dataset (27 (link)) (GPL570 platform), whole blood from 128 patients with MDD samples and 64 healthy individuals was collected. MDD was defined as CORE score >=8. According to the different sources of the samples, we categorized the samples into the RA group, depression group, and normal group, respectively. A simplified workflow of the current investigation is presented in Figure 1.
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Publication 2023
BLOOD Gene Expression Gene Expression Profiling Joints Patients Synovial Membrane Transcriptome
The technique follows the general steps of the original technique described by Guimberteau9 (link) with modifications that avoid division of the ulnar artery, making it more attractive to hand surgeons. Through a Bruner incision, the remnants of the FPL are resected from the digital canal in the thumb, preserving the oblique pulley if present, and otherwise reconstructing the annular pulley. The FDS of the fourth finger is exposed from the distal forearm to the proximal interphalangeal joint level through a zigzag incision. The branch of the ulnar artery to the common carpal synovial sheath is identified and preserved (Fig. 1). The tendon and its investing synovial tissue are elevated based on this vascular branch. The FDS IV tendon is cut proximally at the myotendinous junction and distally at the proximal interphalangeal joint level. Care is taken not to disrupt the synovial tissue around the tendon. Injuries of the synovial sheath are sutured with 8/0 nylon. A sublimus sling is performed in the donor fourth finger to avoid a swan-neck deformity. The pedicled tendon flap is transferred to the thumb, deep to the median nerve, without dividing the ulnar artery (Fig. 2). The distal end of the FDS is pulled through the digital canal with a tendon passer. Care is taken not to strip the synovial envelope of the tendon when passing under the oblique pulley. If an annular pulley is reconstructed, the tension is adjusted to avoid overconstriction of the vascularity of the synovial sheath. Distal fixation is performed with a pull-out transosseous suture to the P2 and a proximal Pulvertaft repair is performed to the FPL at the distal forearm. Full flexion of the thumb with the wrist in neutral is needed to avoid undue tension on the vascular pedicle. If tension is a concern, the ulnar vascular bundle can be separated from the ulnar nerve and mobilized radially. The deep palmar branch of the ulnar artery does not need to be ligated. Enough mobilization is possible through ligation of more proximal minor branches. The volar carpal retinaculum is reconstructed with a retinacular flap (Fig. 3).
The hand is immobilized in a dorsal splint with the wrist in neutral position, the thumb metacarpal adducted, and the metacarpophalanx joint fully flexed. Passive range of movement exercises are started on postoperative day two. Protected active range of movement exercises are started postoperatively at week five and continued until week 12. After the 12th week, full active flexion is allowed (Fig. 4).
Publication 2023
Arecaceae Arteries, Ulnar Blood Vessel Bones, Metacarpal Congenital Abnormality Forearm Injuries Joints Ligation Movement Myotendinous Junction Neck Nerves, Median Nylons Passive Range of Motion Pedicled Flap Pulp Canals Splints Surgeons Surgical Flaps Sutures Synovial Membrane Tendons Thumb Tissue Donors Ulnar Nerve Wrist Wrist Joint
We retrospectively analyzed patients with metastatic STS treated with antiangiogenic agents plus PD-1 inhibitors in Henan Cancer Hospital, the Fifth Affiliated Hospital of Zhengzhou University, and Henan Provincial People’s Hospital between June 2019 and May 2022. This study complied with the principles of Helsinki, met the requirements of the ethics committee and was approved by the ethics committees of each institute. All participants provided written informed consent before treatment.
Patients were included according to the main criteria: 1. Age 18 to 70; 2. The performance status of Eastern Tumor Cooperative Group (ECOG) is 0-2; 3. The pathological diagnosis included ASPS, UPS, synovium, LMS, epithelioid sarcoma (ES), fibrosarcoma, etc. 4. At least one measure based on the Response Evaluation Criteria for Solid Tumors (RECIST) 1.1; 5. Complete medical history and follow-up records; 6. Not eligible for or refusing first-line chemotherapy; and 7. Progressive disease within 6 months before combination treatment. Patients were excluded if they presented contraindications of antiangiogenic agents and/or PD-1 inhibitors including coagulation dysfunction, active asthma, etc.
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Publication 2023
4-maleimido-2,2,6,6-tetramethylpiperidinooxyl Angiogenesis Inhibitors ASIP protein, human Asthma Coagulation, Blood Diagnosis Electrocorticography Ethics Committees Fibrosarcoma Neoplasms Patients Pharmacotherapy Programmed Cell Death Protein 1 Inhibitor Sarcoma, Epithelioid Synovial Membrane
Synovial samples were obtained intra-operatively from 147 OA patients and 60 RA patients. As per the study protocol, orthopedic surgeons were requested to preferentially obtain a research sample from grossly abnormal-looking synovium. Tissue for histological examination was chosen by a pathologist on the basis of gross features including the smoothness and granularity of the synovial surface, red or brown discoloration, and the clarity, dullness, or opacity of the synovial layer, preferentially avoiding regions of electro-cautery effect.
Synovial samples were preferentially obtained from the most grossly inflamed (dull and opaque) area of the synovium. If there was no obviously inflamed synovium, samples were obtained from standard locations: the femoral aspects of the medial and lateral gutters and the central supratrochlear region of the suprapatellar pouch. OA synovial tissue samples were formalin-fixed and paraffin-embedded, and the RA tissues were fresh-frozen in optimal cutting temperature compound. Each tissue biopsy was sectioned at 5-μm thickness and stained with Harris-modified hematoxylin solution and eosin Y (H&E) manufactured by Epredia in Kalamazoo, MI. An expert musculoskeletal pathologist (ED) scored fourteen synovial histologic features in a single section for each patient: lymphocytic inflammation, mucoid change, fibrosis, fibrin, germinal centers, lining hyperplasia, neutrophils, detritus, plasma cells, binucleated plasma cells, Russell bodies, sub-lining giant cells, synovial lining giant cells, and mast cells. Detailed methods for scoring these features are included in the Appendix, some of which are described in prior studies [8 ] and available at www.hss.edu/pathology-synovitis.
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Publication 2023
Biopsy Cauterization Cytoplasmic Granules Eosin Femur Fibrin Fibrosis Formalin Freezing Germinal Center Giant Cells Human Body Hyperplasia Inflammation Lymphocyte Mast Cell Neutrophil Orthopedic Surgeons Paraffin Pathologists Patients Plasma Cells Synovial Membrane Synovitis Tissues

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Collagenase type II is a purified enzyme derived from Clostridium histolyticum. It is used for the dissociation of a variety of cell types, particularly those with a high collagen content, such as cartilage and connective tissue.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

More about "Synovial Membrane"

The synovial membrane, also known as the synovial lining or synovial layer, is a thin, specialized connective tissue that lines the inner surface of joints, tendon sheaths, and bursae.
This highly vascular membrane plays a crucial role in reducing friction and facilitating smooth movement within these structures.
The synovial membrane is responsible for producing synovial fluid, a lubricating and nourishing liquid that helps maintain the health of the surrounding cartilage.
Understanding the structure and function of the synovial membrane is essential for researching joint diseases, such as osteoarthritis and rheumatoid arthritis, and developing effective therapies.
Researchers often utilize various cell culture and tissue engineering techniques to study the synovial membrane, including the use of cell culture media like Fetal Bovine Serum (FBS) and Dulbecco's Modified Eagle Medium (DMEM), as well as reagents like TRIzol for RNA extraction and Collagenase for tissue digestion.
To streamline your synovial membrane research, PubCompare.ai's advanced AI-driven platform can provide you with access to the best protocols and methodologies from literature, preprints, and patents.
By leveraging AI-powered comparisons, you can identify the most accurate and reproducible approaches, helping you drive meaningful insights and accelerate your research progress.
Whether you're studying the synovial membrane's role in joint health or exploring new therapeutic interventions, PubCompare.ai's tools can be invaluable in your research journey.