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Thoracic Aorta

The thoracic aorta is the section of the aorta located within the thoracic cavity.
It begins at the aortic arch and extends to the diaphragm, supplying blood to the chest and abdominal organs.
Resarch on the thoracic aorta is critical for understanding and treating conditions like aortic aneurysms, dissection, and stenosis.
PubCompare.ai helps streamline this research by using AI to identify the best protocols from literature, preprints, and patents, enhancign reproducibility and accuracy.
This innovative platform optimizes thoracic aorta studies to advance medical knowledge and improve patient outcomes.

Most cited protocols related to «Thoracic Aorta»

1
Effects of Aircraft Noise on Glucose Levels
All human data was collected in accordance with the declaration of Helsinki and Ethical approval was granted by the Landesärztekammer Rheinland-Pfalz (Mainz, Germany; permit number: 837.190.12 (8291-F)). Written consent was received from all included individuals. Human data are previously unpublished results of the FLuG-Risiko study.7 (link) Serum of each individual was sampled after nights without or with aircraft noise exposure for 6 h. The aircraft noise consisted of 60 repetitive noise events, which had been recorded near Düsseldorf airport, interrupted by random silent periods, yielding a mean sound pressure level of 47 dB(A) (compared to 39 dB(A) in the silent nights). The detailed protocol and health status of included individuals was published previously.7 (link)
All animals were treated in accordance with the Guide for the Care and Use of Laboratory Animals as adopted by the U.S. National Institutes of Health and approval was granted by the Ethical Committee of the University Medical Center Mainz and the Landesuntersuchungsamt Rheinland-Pfalz (Koblenz, Germany; permit number: 23 177-07/G 15-1-094). After the indicated duration and protocol of noise exposure, animals were killed under isoflurane anaesthesia by transection of the diaphragm and removal of the heart and thoracic aorta. Glucose levels were assessed in whole blood using the ACCU-CHEK Sensor system from Roche Diagnostics GmbH (Mannheim, Germany).
For detailed description of all methods, see Supplementary material online.
Kröller-Schön S., Daiber A., Steven S., Oelze M., Frenis K., Kalinovic S., Heimann A., Schmidt F.P., Pinto A., Kvandova M., Vujacic-Mirski K., Filippou K., Dudek M., Bosmann M., Klein M., Bopp T., Hahad O., Wild P.S., Frauenknecht K., Methner A., Schmidt E.R., Rapp S., Mollnau H, & Münzel T. (2018). Crucial role for Nox2 and sleep deprivation in aircraft noise-induced vascular and cerebral oxidative stress, inflammation, and gene regulation. European Heart Journal, 39(38), 3528-3539.
Publication 2018
Anesthesia Animals Animals, Laboratory BLOOD Diagnosis Glucose Heart Homo sapiens Isoflurane Pressure Serum Sound Thoracic Aorta Vaginal Diaphragm
2
Mouse Aortic Ring Angiogenesis Assay
Angiogenesis was studied by culturing rings of mouse aorta in three-dimensional collagen gels with some modifications of the method originally reported for the rat aorta (4 (link)). Thoracic aortas were removed from mice sacrificed by cervical dislocation and immediately transferred to a culture dish containing ice-cold serum-free Minimum Essential Medium (MEM, Life Technologies Ltd., Paisley, Scotland). The peri-aortic fibroadipose tissue was carefully removed with fine microdissecting forceps and iridectomy scissors paying special attention not to damage the aortic wall. One millimeter long aortic rings (approximately 15 per aorta) were sectioned and extensively rinsed in 5 consecutive washes of MEM. Ring-shaped explants of mouse aorta were then embedded in a rat tail interstitial collagen gel (1.5 mg/ml) (15 (link)) prepared by mixing 7.5 volumes of 2 mg/ml collagen (Collagen R, Serva, Heidelberg, Germany), 1 volume of 10 x MEM, 1.5 volume of NaHCO3 (15.6mg/ml) and approximately 0.1 volume of 1M NaOH to adjust the pH to 7.4. The collagen gels containing the aortic rings were polymerized in cylindrical agarose wells prepared as previously described (4 (link)) and kept in triplicate at 37°C in 60 mm diameter Petri dishes (bacteriological polystyrene, Falcon, Becton Dickinson, Lincoln Park, New Jersey). Each dish contained 6 ml of MCDB131 (Life technologies Ltd., Paisley, Scotland) supplemented with 25 mM NaHCO3, 2.5% mouse serum, 1% glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. The cultures were kept at 37°C in a humidified environment for a week and examined every second day with an Olympus microscope at appropriate magnification.
Masson V., Devy L., Grignet-Debrus C., Bernt S., Bajou K., Blacher S., Roland G., Chang Y., Fong T., Carmeliet P., Foidart J.M, & Noël A. (2002). Mouse Aortic Ring Assay: A New Approach of the Molecular Genetics of Angiogenesis. Biological Procedures Online, 4, 24-31.
Publication 2002
angiogen Aorta Attention Bicarbonate, Sodium Collagen Common Cold Culture Media Forceps Gels Glutamine Hyperostosis, Diffuse Idiopathic Skeletal Iridectomy Joint Dislocations Mice, House Microscopy Neck Penicillins Polystyrenes Sepharose Serum Streptomycin Tail Thoracic Aorta Tissues
3
Comprehensive Cardiac Calcium Quantification

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Publication 2016
Aortic Stenosis, Calcific Blood Vessel Calcium Electricity Electron Beam Computed Tomography Heart Mitral Valve Multidetector Computed Tomography Patients Radionuclide Imaging Thoracic Aorta X-Ray Computed Tomography
4
Isolation and Culture of Vascular Smooth Muscle Cells
Under sterile conditions, anaesthetized SD rats were placed in the supine position and their chests were opened. The thoracic aorta was removed and transferred to a culture dish with cold (4°C) DMEM (Figure 1A). After removal of the fat tissue around the artery (Figure 1B), the artery was longitudinally cut and placed in another cell culture dish containing DMEM (Figure 1C). Then, we used a pair of ophthalmic curved tweezers to scrape the intima softly to get rid of endothelial cells (Figure 1D). Later, we did not separate the adventitia or directly cut the artery into small tissue blocks. Two pairs of ophthalmic curved tweezers were used: one to press the artery to fix it and another to separate the media from the artery by pressing and pushing the artery with its blunt back side (Figure 2). After half of the media was removed, the same method was used to get the other half. Then, the media was cut into approximately 1-mm squares and transferred into cell culture plates. The plates were placed in a cell culture chamber for about 4 h to let the small tissue blocks adhere to the plates. DMEM containing 20% FBS was carefully added and the tissue blocks were incubated in the cell culture chamber without disturbance for the first 5 days.
In the traditional tissue explants method, all the steps were similar, and after the fat tissue was removed, the artery was directly cut into small tissue blocks and transferred to cell culture plates without removal of the adventitia.
In the enzyme digestion method, after the fat tissue was removed, the aorta was digested with 1 mg/mL collagenase II and 100 μg/mL elastase at 37°C for 1 h. Later, the cells were pelleted and plated in DMEM with 20% FBS. The next morning, the cells were washed with PBS 3 times and the media were refreshed every 48 h.
The A7r5 cell line has been widely used in vitro to study the physiology and pathophysiology of VSMCs [13 (link),14 (link)]. However, it has lost some VSMCs selectivity and has many differences from primary cultured VSMCs. Therefore, we chose the A7r5 cell line to compare its viability with primary cultured VSMCs.
The VSMCs obtained by the above methods (the new tissue explants method, the traditional tissue explants method, the enzyme digestion method, and A7r5 cell line) were identified through morphology and immunofluorescence detection of SM-actin. The purity of the VSMCs was tested through multiple fluorescent staining with DAPI and SM-actin antibody.
Chi J., Meng L., Pan S., Lin H., Zhai X., Liu L., Zhou C., Jiang C, & Guo H. (2017). Primary Culture of Rat Aortic Vascular Smooth Muscle Cells: A New Method. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 23, 4014-4020.
Publication 2017
Actins Adventitia Aorta Arterial Media Arterial Occlusion Arteries Cell Culture Techniques Cell Lines Cells Chest Collagenase Common Cold Cultural Evolution DAPI Digestion Endothelial Cells Enzymes Fluorescent Antibody Technique Genetic Selection Hyperostosis, Diffuse Idiopathic Skeletal Immunoglobulins Pancreatic Elastase physiology Rattus norvegicus Sterility, Reproductive Thoracic Aorta Tissue, Adipose Tissues Tunica Intima Type II Mucolipidosis
5
Mapping Mechanical Properties of Human Aorta

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Publication 2019
Adventitia Aneurysm Aorta Caucasoid Races Males Normal Saline Phosphates Radius Subclavian Artery Thoracic Aorta Tunica Intima

Most recents protocols related to «Thoracic Aorta»

1
Angiotensin II-Induced Gene Expression
The effects of αv knockout on gene expression were examined at 6 weeks after tamoxifen injection followed or not by a 4-week treatment with Ang II. Anaesthesia was induced by isoflurane inhalation at 3.5% in 1 L/min oxygen, and then maintained at 1.5% in 1 L/min oxygen during the intervention. Angiotensin II was administered at 1.5 mg/kg/day for 28 days via subcutaneous Alzet osmotic minipumps (Charles River, L’Arbresle, France). Prior to pump implantation, mice were conditioned to tail-cuff blood pressure measurement using a computerized mouse tail-cuff sphygmomanometer (Hatteras Instruments, Inc., NC, USA). Systolic arterial pressure (SAP) and heart rate (HR) were measured on the day of surgery and on day 28 prior to euthanasia. Mice were euthanized via exsanguination under isoflurane anaesthesia (1.5% in 1 L/min oxygen). Carotid arteries were used for morphological and histological analyses and thoracic aorta for all other parameters. It is generally accepted including our previous studies that changes in gene and/or protein expression are similar in these two main conductance arteries.14 (link),15 (link)
Li Z., Belozertseva E., Parlakian A., Bascetin R., Louis H., Kawamura Y., Blanc J., Gao-Li J., Pinet F., Lacy-Hulbert A., Challande P., Humphrey J.D., Regnault V, & Lacolley P. (2023). Smooth muscle αv integrins regulate vascular fibrosis via CD109 downregulation of TGF-β signalling. European Heart Journal Open, 3(2), oead010.
Publication 2023
Anesthesia Angiotensin II Arteries Carotid Arteries Determination, Blood Pressure Euthanasia Exsanguination Gene Expression Genes Inhalation Isoflurane Mus Osmosis Ovum Implantation Oxygen Proteins Rate, Heart Rivers Sphygmomanometers Surgery, Day Systolic Pressure Tail Tamoxifen Thoracic Aorta
2
Outcomes of Aortic Arch Repair with Jotec E-vita Open NEO
We reviewed all consecutive patients who underwent TAR FET with the Jotec E-vita Open NEO™ hybrid prosthesis for complex aortic arch disease in two different Asian institutes, Gangnam Severance Hospital (GSH) and Prince of Wales Hospital (PWH), between October 2020 and August 2021. Preoperative characteristics of the patients are shown in Table 1.
Indications of TAR FET in both institutions align with the European Association for Cardio-Thoracic Surgery position statement on the conduct of the FET procedure (10 (link),11 (link)). The indications include: (I) acute type A aortic dissection or intramural hematoma with entry tear in the aortic arch or proximal descending thoracic aorta, (II) descending aortic dissection type B without adequate landing zone and/or when endovascular treatment is contraindicated and/or with non-A non-B dissection (14 (link)), or (III) acute rupture of arch aneurysm, or (IV) ascending thoracic aortic aneurysm or arch aneurysm with descending thoracic aortic pathologies such as dissection, aneurysm . In both institutions, computed tomography (CT) aortogram prior to the surgery and a follow-up CT aortogram after chest drain removal postoperatively were performed.
The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). The institutional review boards of both institutes approved this retrospective cohort study (PWH IRB No. 2021.573, 4/11/2021; GSH IRB No. 2021-0421-010 3-2021-0359), and individual consent for this retrospective analysis was waived.
Ho J.Y., Kim C.H., Chow S.C., Kwok M.W., Lee H., Kim T.H., Fujikawa T., Wong R.H, & Song S.W. (2023). Initial Asian experience of the branched E-vita open NEO in complex aortic pathologies. Journal of Thoracic Disease, 15(2), 484-493.
Publication 2023
Aneurysm Aortic Aneurysm, Thoracic Aortic Diseases Aortography Arch of the Aorta Asian Persons Chest Tubes Descending Thoracic Aortic Aneurysm Dissecting Aneurysms Dissection Europeans Hematoma Hybrids Laceration Limb Prosthesis Operative Surgical Procedures Patients Thoracic Aorta Thoracic Surgical Procedures Vita-E X-Ray Computed Tomography
3
Aortic Tissue Extraction for Cell Culture
Ascending aortic specimens were obtained intra-operatively from 40 consecutive cardiac patients undergoing aortic valve surgery. A 4–5 mm wide circumferential strip of aortic tissue was excised 5–10 mm above the sinotubular junction from the anterior circumference of the thoracic aorta, adjacent to the aortotomy. Aortic valve morphology was determined pre-operatively by either trans-esophageal or trans-thoracic echocardiography, and only normal (i.e., tricuspid) aortic valves were included. Aortic valve morphology was confirmed intra-operatively by the surgeon. Aortic dimensions were determined pre-operatively by computed tomography, and confirmed intra-operatively by trans-esophageal echocardiography. Aortic samples extracted in the operating room were divided and were either immediately fixed in 4% phosphate-buffered formalin for histological studies, or were placed in PBS and directly transferred to a sterile tissue culture hood for immediate extraction of vascular SMCs.
Balint B., Bernstorff I.G., Schwab T, & Schäfers H.J. (2023). Age-dependent phenotypic modulation of smooth muscle cells in the normal ascending aorta. Frontiers in Cardiovascular Medicine, 10, 1114355.
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Publication 2023
Aorta Blood Vessel Echocardiography Echocardiography, Transesophageal Formalin Heart Operative Surgical Procedures Patients Phosphates Sinotubular Junction Sterility, Reproductive Surgeons Thoracic Aorta Tissues Valves, Aortic X-Ray Computed Tomography
4
Evaluating Aortic Endothelial Function in Mice
Wildtype male mice (n = 4, 12–15 weeks of age) were euthanized by overdose inhalation of isoflurane. Descending thoracic aortas were removed from mice and placed in ice-cold Krebs Hepes buffer while cleaned of adherent fat and connective tissues. Krebs Hepes buffer (pH 7.4, 37 °C) was composed of 114 mM NaCl, 4.7 mM KCl, 0.8 mM KH2PO4, 1.2 mM MgCl2 6H2O, 2.5 mM, CaCl2 2H2O, 11.0 mM D-Glucose, 20 mM NaHCO3, and 5 mM Hepes hemisodium salt. Krebs buffer was bubbled continuously with 95% O2/5% CO2 during myograph experiments. In brief, we used DMT 620M myograph chambers with the methods and conditions described in (41 ) for continuous measuring and recording of isometric tension with mouse aortas. The aorta from each mouse was divided into two groups: control (vehicle, PBS) and treatment (rhFABP3, 45 ng/ml). We tested the viability of aorta preparations (1–3 mm lengths) using 90 mM KCl. Viable tissues contractions were >1 mN. We assessed acetylcholine-induced relaxations of phenylephrine-contracted aortas under isometric tension conditions as we described previously (41 ). Aortic rings mounted in the DMT620 M chambers were exposed to treatments for 20 min then contracted with phenylephrine (3 μM) and then acetylcholine dose–responses curves constructed.
Nguyen H.C., Bu S., Nikfarjam S., Rasheed B., Michels D.C., Singh A., Singh S., Marszal C., McGuire J.J., Feng Q., Frisbee J.C., Qadura M, & Singh K.K. (2023). Loss of fatty acid binding protein 3 ameliorates lipopolysaccharide-induced inflammation and endothelial dysfunction. The Journal of Biological Chemistry, 299(3), 102921.
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Publication 2023
Acetylcholine Aorta Bicarbonate, Sodium Buffers Common Cold Connective Tissue Drug Overdose Glucose HEPES Inhalation Isoflurane Magnesium Chloride Males Mus Myography Phenylephrine Sodium Chloride Thoracic Aorta Tissues
5
Post-CABG Anatomy 3D Reconstruction
Post-operative CCTA imaging data of n = 2 patients were retrospectively acquired from the University of Nebraska Medical Center imaging database (S1 Table). To 3D reconstruct the post-CABG anatomy, the path lines of the ascending aorta, aortic arch, descending thoracic aorta, great vessels, coronary arteries (including their major branches), and bypass grafts were extracted. The lumen boundaries were segmented manually using an open-source software (SimVascular, Stanford, California, USA) [26 (link)]. The segmented lumen contours were lofted to create the 3D patient-specific lumen geometries. The 3D reconstructed lumen geometries were discretized into tetrahedral elements using the SimVascular software. Based on a mesh independence analysis that was conducted until a further mesh refinement resulted in less than 5% change in the calculated wall shear stress (WSS), we applied the following mesh resolution: 3 mm in the aorta, 1.5 mm in aortic branches, 0.1–0.3 mm in coronary arteries and grafts. The average total number of elements was 2.1 million.
Wu W., Panagopoulos A.N., Vasa C.H., Sharzehee M., Zhao S., Samant S., Oguz U.M., Khan B., Naser A., Harmouch K.M., Kassab G.S., Siddique A, & Chatzizisis Y.S. (2023). Patient-specific computational simulation of coronary artery bypass grafting. PLOS ONE, 18(3), e0281423.
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Publication 2023
Aorta Arch of the Aorta Artery, Coronary Ascending Aorta Blood Vessel Coronary Artery Bypass Surgery Grafts Patients Thoracic Aorta

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More about "Thoracic Aorta"

The thoracic aorta is the section of the aorta located within the thoracic cavity, also known as the chest or thorax.
It begins at the aortic arch and extends down to the diaphragm, supplying blood to the organs and tissues in the chest and abdomen.
Conditions like aortic aneurysms, dissections, and stenosis (narrowing) can affect the thoracic aorta, and research in this area is crucial for understanding and treating these life-threatening conditions.
PubCompare.ai is an innovative platform that utilizes artificial intelligence (AI) to streamline and optimize thoracic aorta research.
By identifying the best protocols from literature, preprints, and patents, PubCompare.ai can enhance the reproducibility and accuracy of studies, leading to advancements in medical knowledge and improved patient outcomes.
Researchers studying the thoracic aorta may also encounter terms like fetal bovine serum (FBS), Dulbecco's Modified Eagle's Medium (DMEM), TRIzol reagent, Matrigel, penicillin/streptomycin, penicillin, streptomycin, and the RNeasy Mini Kit, which are commonly used in cell culture and molecular biology experiments.
Image-Pro Plus 6.0 is a software tool that can be used for image analysis and quantification.
By leveraging the power of AI and the latest research, PubCompare.ai helps researchers navigate the complexities of thoracic aorta studies, leading to more efficient and effective investigations that can ultimately improve patient care and outcomes.
With its user-friendly interface and robust analytical capabilities, this innovative platform is a valuable resource for clinicians and researchers in the field of cardiovascular medicine.