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Tibia

Tibia: The larger of the two lower leg bones, extending from the knee to the ankle.
It is responsible for supporting the body's weight and facilitating movement.
Researchers can leverage AI-driven comparisons from PubCompare.ai to identify the best protocols and products for Tibia studies, enhancing reproducibility and accuracy in their research.
This powerful platfrom provides access to published literature, pre-prints, and patents, optimizing Tibia research through data-driven insights.

Most cited protocols related to «Tibia»

Bone marrow cells were collected from the femurs and tibiae of wild-type BALB/c mice (Taconic) by flushing the opened bones with Iscove's Modified Dulbecco's Medium (IMDM; Invitrogen). Red blood cells were lysed in dH2O followed by the addition of 10X PBS. After centrifugation, the cells were washed once in PBS containing 0.1% BSA. The bone marrow cells were cultured at 106/mL in media containing RPMI 1640 (Invitrogen) with 20% FBS (Cambrex), 100 IU/mL penicillin and 10 μg/mL streptomycin (Cellgro), 2 mM glutamine (Invitrogen), 25 mM HEPES and 1x non-essential amino acids and 1 mM sodium pyruvate (Gibco) and 50 μM β-mercaptoethanol (Sigma) and supplemented with 100 ng/mL stem-cell factor (SCF; PeproTech) and 100 ng/mL FLT3-Ligand (FLT3-L; PeproTech) from day 0 to day 4. On day 4, the media containing SCF and FLT3-L was replaced with media containing 10 ng/mL recombinant mouse interleukin-5 (rmIL-5; R&D Systems) only. On day 8, the cells were moved to new flasks and maintained in fresh media supplemented with rmIL-5. Every other day, from this point forward, one-half of the media was replaced with fresh media containing rmIL-5, and the concentration of the cells was adjusted each time to106 /mL. Cells were enumerated at day 0 and on days indicated thereafter in a hemocytometer, and 50,000 cells were subjected to cytospin (Thermo Shandon, Pittsburgh, PA). The cytospin preparations were fixed and stained using a modified Giemsa preparation (Diff Quik, Dade Behring AG, Dudingen, Switzerland).
Publication 2008
2-Mercaptoethanol 3,3'-diallyldiethylstilbestrol Amino Acids, Essential Bone Marrow Cells Bones Cells Centrifugation Diff Quik Erythrocytes Femur flt3 ligand FLT3 protein, human Glutamine HEPES Interleukin-5 Mice, Inbred BALB C Mus Penicillins Pyruvate Sodium Stain, Giemsa Stem Cell Factor Streptomycin Tibia
After IRB approval, we collected tibial plateau specimens from 78 individuals with osteoarthritis that were undergoing total knee replacement surgery. The specimens were processed for μCT, ELISA and histological examination. We purchased healthy knees specimens from the Nation Disease Research Interchange (NDRI) to serves as controls.
Publication 2013
Degenerative Arthritides Enzyme-Linked Immunosorbent Assay Knee Knee Replacement Arthroplasty Operative Surgical Procedures Tibia
After IRB approval, we collected tibial plateau specimens from 78 individuals with osteoarthritis that were undergoing total knee replacement surgery. The specimens were processed for μCT, ELISA and histological examination. We purchased healthy knees specimens from the Nation Disease Research Interchange (NDRI) to serves as controls.
Publication 2013
Degenerative Arthritides Enzyme-Linked Immunosorbent Assay Knee Knee Replacement Arthroplasty Operative Surgical Procedures Tibia

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Publication 2011
Acceleration Adult Biceps Femoris Cadaver Cerebral Palsy Child Epistropheus Femur Foot Generic Drugs Gomphosis Gravitation Gravity Head Hip Joint Joints Joints, Ankle Knee Joint Muscle, Gastrocnemius Muscle Tissue Pelvis Plant Roots Quadriceps Femoris Rectus Femoris Semimembranosus Tibia Torso Vastus Intermedius Vastus Lateralis Vastus Medialis Vertebrae, Lumbar

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Publication 2010
Arecaceae Carbon Fiber CD3EAP protein, human Durapatite Forearm Leg Patients Radionuclide Imaging Radius Skeleton Tibia Tomography

Most recents protocols related to «Tibia»

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Example 1

Provided herein is an exemplary embodiment of workflow for tracking and registering a knee joint using markers that are drilled into the in tibia and femur of the knee joint in the patient and protrude out from their placement site. The placement of the marker in order to track and register the bones of the knee joint is an invasive procedure that damages the tissue at and around the knee joint. The marker is used in marker-based tracking to track and register the knee joint and in robot-assisted surgical systems. Such invasive fixation of markers to bones may lead to complications, infections, nerve injury, and bone fracture. The marker fixation may reduce the flexibility during the procedure as the protruding markers may get in the way during the procedure. The surgical procedure may take longer to fix the marker into place than a markerless approach.

Patent 2024
Bones Femur Fracture, Bone Infection Injuries Knee Joint Nervousness Operative Surgical Procedures Patients Robotic Surgical Procedures Tibia Tissues
Referring to the common experimental animal randomization method and the 3R principle, 36 C57BL/6 male mice (6 weeks old) were randomized into the following three groups: a sham surgery group (incision of the right knee, lack of ACL transection surgery) and two groups in which the right knee articular cartilage underwent anterior cruciate ligament transection (ACLT) to establish an animal model of OA (n = 12). The two OA experimental groups were administered intra-articular injections of either AAV-miR-760 mimic or AAV-miR-760 mimic + AAV-OE HBEGF immediately after ACLT surgery (0 weeks). The injection procedure was repeated after 4 weeks, and the mice were sacrificed at 4 weeks after the second injection. The right knee joints of each group were harvested for the extraction of RNA (n = 3) and proteins (n = 3), with OA progression being assessed based on the expression of HBEGF, MMP3, MMP13, ADAMTS4, COL2A1, and Aggrecan. Briefly, mice were sacrificed with the nape facing up. Then, the front legs were immobilized and the skin and soft tissue were removed on the hind leg to make an incision at the knee joint. After exposing the tibial plateau, the surface resembling a regular translucent sphere (articular cartilage) was severed and processed for RNA and protein studies. In addition, the right knee joints of the remaining mice (n = 6) were dissected and processed for safranin-O/fast green staining and immunohistochemistry staining.
Publication 2023
Aggrecans Animal Model Animals, Laboratory Anterior Cruciate Ligament Cartilages, Articular Disease Progression Fast Green Heparin-binding EGF-like Growth-Factor Intra-Articular Injections Knee Joint Males Mice, Inbred C57BL Mice, Laboratory MMP3 protein, human MMP13 protein, human Operative Surgical Procedures Proteins safranine T Skin Tibia Tissues
The paraffin-embedded tissue sections were deparaffinized and rehydrated following standard procedures. Sections were incubated with 3% H2O2 to block endogenous peroxidase activity and antigen retrieval was performed in citrated buffer at 110 ℃, for 5 min in a pressure cooker. After the citrated buffer reached room temperature, the sections were removed and incubated overnight with the primary antibodies COL2A1 (1:200, bioss, bs-10589R) and SOX9 (1:1000, Abcam, Cat# ab185966) at 4 ℃, followed by incubation with an HRP conjugated secondary antibody (Beyotime Institute of Biotechnology, Inc., Nantong, China) for 2 h at room temperature. Peroxidase binding for both COL2A1 and SOX9 was visualized using diaminobenzidine. Then, the nuclei were counterstained with hematoxylin, while the slides were dehydrated, mounted, and analyzed with a light microscope. For the quantitative analysis, all positively stained cells, including those in the femoral condyle and tibial plateau area, on the articular surface per specimen were counted, and the percentage of positive cells was calculated using Image-Pro Plus 6.0.
Publication 2023
Antibodies Antigens Buffers Cardiac Arrest Cell Nucleus Condyle Femur Hematoxylin Immunoglobulins Joints Light Microscopy Paraffin Peroxidase Peroxide, Hydrogen Pressure SOX9 protein, human Tibia Tissues
As determined by the individual situation, an autogenous tricortical bone graft of appropriate size was harvested from the ipsilateral iliac crest. Cancellous bone was harvested with the smallest osteotome possible. A longitudinal dorsal incision was made lateral to the extensor hallucis longus tendon with an interface between the extensor hallucis longus tendon and the dorsalis pedis artery, both of which were retracted correspondingly. The soft tissue was distracted by a lamina spreader to expose the talonavicular and navicular-cuneiform joints. The talonavicular and navicular-cuneiform joints were distracted using a Hintermann distractor over separate K-wires. The articular surfaces were debrided in situ with cartilage shovels to the subchondral bone. A K-wire was used to drill the subchondral sclerotic bone plate into a favaginous condition for fusion. Then bite off the excess osteophyte from the lateral 4-corners. The plantar ligament and plantar soft tissue of the navicular are loosened with a sharp knife, leaving only the insertion point of the posterior tibial tendon. The whole debridement process created a relative space around the navicular bone. Subsequently, a periosteal detacher was pressed against the lateral bony protrusion of the navicular bone to rotate the bone outwards to the original top location. Once the reduction was deemed satisfactory by direct visualization, two to three crossing K-wires were used for temporary fixation. After the demonstration of the corrected medial longitudinal arch on the C-arm, the lateral half of the navicular bone (including the talonavicular and navicular-cuneiform joints involved in the necrotic lesion) was excised using an osteotome to form a broad dorsal trapezoid laterally and a rectangular longitudinal bone bed. And the modified tricortical iliac bone block was inserted into the space between the talus and the cuneiforms. Finally, two hollow lag screws and a dorsal LCP were used to arthrodese the talonavicular-cuneiform joints. A transverse Herbert screw was used (where needed) to fix the bone block and medial navicular bone. The wound was closed after packing the previously acquired cancellous bone to smooth the defect gaps.
Postoperatively, a protective non-weight bearing short-leg plaster cast was applied for 6 weeks, after which weight-bearing was gradually allowed as tolerated.
Publication 2023
Arteries Arthrodesis Bone Diseases Bones Bone Transplantation Cancellous Bone Cartilage Debridement Dental Occlusion Drill Iliac Crest Ilium Joints Kirschner Wires Navicular Bone of Foot Necrosis Osteophyte Osteotomy Periosteum Plantar Plate Plaster Casts Scaphoid Bone Sclerosis Talus Tendons Tibia Tissues Trapezoid Bones Wounds
Patients were eligible if they presented with a confirmed distal radius, ankle, femoral shaft, tibial shaft, distal femur, or proximal tibia fracture requiring surgery, were aged > 18 years, were able to speak and read the English language, and had capacity to make healthcare decisions independently. Patients were deemed ineligible if they were medically diagnosed with cognitive impairment or were otherwise incapacitated, had limited literacy, or had revision surgery.
Publication 2023
Ankle Disorders, Cognitive Femur Operative Surgical Procedures Patients Radius Repeat Surgery Tibia Tibial Fractures

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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M-CSF is a recombinant human macrophage colony-stimulating factor (M-CSF) that promotes the proliferation and differentiation of macrophages from hematopoietic progenitor cells. It functions as a cytokine and is involved in the regulation of macrophage production and function.
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GM-CSF is a laboratory reagent used for cell culture applications. It is a recombinant human granulocyte-macrophage colony-stimulating factor that promotes the growth and differentiation of hematopoietic cells.
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
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α-MEM is a cell culture medium formulated for the growth and maintenance of mammalian cells. It provides a balanced salt solution, amino acids, vitamins, and other nutrients required for cell proliferation.
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.

More about "Tibia"

The tibia, also known as the shinbone or the larger of the two lower leg bones, plays a crucial role in supporting the body's weight and facilitating movement.
This important bone extends from the knee to the ankle, providing a sturdy foundation for everyday activities.
Researchers studying the tibia can leverage the power of AI-driven comparisons from PubCompare.ai to identify the best protocols and products for their research, enhancing reproducibility and accuracy.
This innovative platform provides access to a wealth of published literature, pre-prints, and patents, allowing researchers to optimize their tibia studies through data-driven insights.
By tapping into PubCompare.ai's capabilities, scientists can compare and contrast various methodologies, reagents, and tools used in tibia-related research, such as cell culture media like DMEM, α-MEM, and RPMI 1640, as well as growth factors like M-CSF and GM-CSF.
Additionally, the use of antibiotics like penicillin and streptomycin, along with the amino acid L-glutamine, can be explored to ensure the best possible outcomes in tibia studies.
Enhancing the reproducibility and accuracy of tibia research is essential, and PubCompare.ai's AI-driven insights can help researchers navigate the vast landscape of scientific literature, pre-prints, and patents, optimizing their workflows and ultimately advancing our understanding of this critical bone structure.
Whether you're investigating the biomechanics, development, or pathologies of the tibia, PubCompare.ai is a powerful tool that can elevate your research to new heights.