Thirty-four neuroblastoma cell lines were grown to subconfluency according to standard culture conditions. RNA was isolated using the RNeasy Midi Kit (Qiagen) according to the manufacturer's instructions. Nine RNA samples from pooled normal human tissues (heart, brain, fetal brain, lung, trachea, kidney, mammary gland, small intestine and uterus) were obtained from Clontech. Blood and fibroblast biopsies were obtained from different normal healthy individuals. Thirteen leukocyte samples were isolated from 5 ml fresh blood using Qiagen's erythrocyte lysis buffer. Fibroblast cells from 20 upper-arm skin biopsies were cultured for a short time (3-4 passages) and harvested at subconfluency as described [22 (link)]. Bone marrow samples were obtained from nine patients with no hematological malignancy. Total RNA of leukocyte, fibroblast and bone marrow samples was extracted using Trizol (Invitrogen), according to the manufacturer's instructions.
Uterus
The uterus is a muscular, hollow organ located in the female pelvis that plays a crucial role in the reproductive system.
It is responsible for the implantation and development of a fertilized egg, as well as the expulsion of the fetus during childbirth.
The uterus is composed of three main layers: the endometrium, the myometrium, and the perimetrium.
The endometrium is the inner lining of the uterus that undergoes cyclic changes during the menstrual cycle.
The myometrium is the middle, muscular layer that contracts during childbirth to facilitate the delivery of the fetus.
The perimetrium is the outer, serous layer that covers the uterus.
The uterus is connected to the fallopian tubes, which transport the egg from the ovaries to the uterus.
Disorders of the uterus can include uterine fibroids, endometriosis, and uterine cancer, among others.
Proper understadning and optimization of uterine function is crucial for women's health and reproductive outcomes.
It is responsible for the implantation and development of a fertilized egg, as well as the expulsion of the fetus during childbirth.
The uterus is composed of three main layers: the endometrium, the myometrium, and the perimetrium.
The endometrium is the inner lining of the uterus that undergoes cyclic changes during the menstrual cycle.
The myometrium is the middle, muscular layer that contracts during childbirth to facilitate the delivery of the fetus.
The perimetrium is the outer, serous layer that covers the uterus.
The uterus is connected to the fallopian tubes, which transport the egg from the ovaries to the uterus.
Disorders of the uterus can include uterine fibroids, endometriosis, and uterine cancer, among others.
Proper understadning and optimization of uterine function is crucial for women's health and reproductive outcomes.
Most cited protocols related to «Uterus»
Arm, Upper
Biopsy
BLOOD
Bone Marrow
Brain
Buffers
Cell Lines
Erythrocytes
Fetus
Fibroblasts
Heart
Hematologic Neoplasms
Homo sapiens
Intestines, Small
Kidney
Leukocytes
Lung
Mammary Gland
Neuroblastoma
Patients
Skin
Tissues
Trachea
trizol
Uterus
Images were kindly provided by Javier Frias Aldeguer and Nicolas Rivron of Hubrecht Institute for Developmental Biology and Stem Cell Research and Li Linfeng of MERLN Institute for Technology-Inspired Regenerative Medicine. As per Rivron and colleagues [33 (link)], mouse embryos (3.5 dpc) were fixed right after isolation from the mother’s uterus. Fixation was performed using 4% PFA in RNAse-free PBS containing 1% acetic acid. ViewRNA ISH Cell Assay kit (cat# QVC0001) was used for performing smFISH on the embryos. The protocol includes steps of permeabilization and protease treatment as well as probes, preamplifier, amplifier, and label hybridizations. Embryos were then mounted in Slowfade reagent (Thermofisher cat# S36937) and directly imaged in a PerkinElmer Ultraview VoX spinning disk microscope in confocal mode by using a 63×/1.40 NA oil immersion lens.
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2-5A-dependent ribonuclease
Acetic Acids
Biological Assay
Cells
Crossbreeding
Embryo
isolation
Lens, Crystalline
Microscopy
Mothers
Mus
Peptide Hydrolases
Submersion
Uterus
A vaginal swab was collected using a cotton tipped swab (Puritan Medical Products Company, LLC Guilford, ME) wetted with ambient temperature physiological saline and inserted into the vagina of the restrained mouse. The swab was gently turned and rolled against the vaginal wall and then removed. Cells were transferred to a dry glass slide by rolling the swab across the slide. The slide was air dried and then stained with approximately 400 µL of stain (Accustain, Sigma-Aldrich, St. Louis, MO) for 45 seconds. The slides were rinsed with water, overlaid with a coverslip, and viewed immediately at 200× magnification under bright field illumination. The stage of the estrous cycle was determined based on the presence or absence of leukocytes, cornified epithelial, and nucleated epithelial cells according to Felicio, et al [9] (link).
When the female is in proestrus, mostly nucleated and some cornified epithelial cells are present. Some leukocytes may be present if the female is in early proestrus. As the stage of the cycle advances to estrus, mostly cornified epithelial cells are present. If the cycle is not interrupted by pregnancy, pseudopregnancy, or other phenomena, metestrus will begin. Metestrus is a brief stage when the corpora lutea form but fail to fully luteinize due to a lack of progesterone. The uterine lining will begin to slough and evidence of this is seen in the form of cornified eipithelial cells and polymorphonuclear leukocytes present in vaginal swabs. Some nucleated epithelia cells will also be present in late metestrus. Diestrus is the longest of the stages lasting more than 2 days. Vaginal swabs during diestrus show primarily polymorphonuclear leukocytes and a few epithelial cells during late diestrus. Leukocytes remain the predominant cell type having removed cellular debris. The cycle then repeats.
When the female is in proestrus, mostly nucleated and some cornified epithelial cells are present. Some leukocytes may be present if the female is in early proestrus. As the stage of the cycle advances to estrus, mostly cornified epithelial cells are present. If the cycle is not interrupted by pregnancy, pseudopregnancy, or other phenomena, metestrus will begin. Metestrus is a brief stage when the corpora lutea form but fail to fully luteinize due to a lack of progesterone. The uterine lining will begin to slough and evidence of this is seen in the form of cornified eipithelial cells and polymorphonuclear leukocytes present in vaginal swabs. Some nucleated epithelia cells will also be present in late metestrus. Diestrus is the longest of the stages lasting more than 2 days. Vaginal swabs during diestrus show primarily polymorphonuclear leukocytes and a few epithelial cells during late diestrus. Leukocytes remain the predominant cell type having removed cellular debris. The cycle then repeats.
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Cells
Corpus Luteum
Diestrus
Epithelial Cells
Epithelioid Cells
Estrous Cycle
Estrus
Gossypium
Granulocyte
Leukocytes
Lighting
Metestrus
Mus
Neoplasm Metastasis
physiology
Pregnancy
Proestrus
Progesterone
Pseudocyesis
Saline Solution
Stains
Uterus
Vagina
Vision
Woman
Results are based in part upon data generated by TCGA Research Network (http://cancergenome.nih.gov/ ). We aggregated TCGA transcriptomic and RPPA data from public repositories, listed in the “Data availability” section. RNA-seq expression data were processed by TCGA at the gene level, rather than at the transcript level. Tumors spanned 32 different TCGA projects, each project representing a specific cancer type, listed as follows: LAML, acute myeloid leukemia; ACC, adrenocortical carcinoma; BLCA, bladder urothelial carcinoma; LGG, lower grade glioma; BRCA, breast invasive carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, cholangiocarcinoma; CRC, colorectal adenocarcinoma (combining COAD and READ projects); ESCA, esophageal carcinoma; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; DLBC, lymphoid neoplasm diffuse large B-cell lymphoma; MESO, mesothelioma; OV, ovarian serous cystadenocarcinoma; PAAD, pancreatic adenocarcinoma; PCPG, pheochromocytoma and paraganglioma; PRAD, prostate adenocarcinoma; SARC, sarcoma; SKCM, skin cutaneous melanoma; STAD, stomach adenocarcinoma; TGCT, testicular germ cell tumors; THYM, thymoma; THCA, thyroid carcinoma; UCS, uterine carcinosarcoma; UCEC, uterine corpus endometrial carcinoma; UVM, uveal melanoma. Cancer molecular profiling data were generated through informed consent as part of previously published studies and analyzed per each original study’s data use guidelines and restrictions.
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4-carboxyphenylglyoxal
Adenocarcinoma
Adenocarcinoma of Lung
Adrenocortical Carcinoma
Breast Carcinoma
Carcinoma, Thyroid
Carcinoma, Transitional Cell
Carcinosarcoma
Cells
Cholangiocarcinoma
Chromophobia
Chronic Obstructive Airway Disease
Diffuse Large B-Cell Lymphoma
Endocervix
Endometrial Carcinoma
Esophageal Cancer
Familial Atypical Mole-Malignant Melanoma Syndrome
Gene Expression Profiling
Genes
Glioblastoma Multiforme
Glioma
Hepatocellular Carcinomas
Hypernephroid Carcinomas
Kidney
Leukemia, Myelocytic, Acute
Lung
Lymph
Malignant Neoplasms
Mesothelioma
Neck
Neoplasms
Ovary
Pancreas
Paraganglioma
Pheochromocytoma
Prostate
Renal Cell Carcinoma
RNA-Seq
Sarcoma
Serous Cystadenocarcinoma
Squamous Cell Carcinoma
Squamous Cell Carcinoma of the Head and Neck
Stomach
Testicular Germ Cell Tumor
Thymoma
Urinary Bladder
Uterus
Uveal melanoma
X-Ray Photoelectron Spectroscopy
2',5'-oligoadenylate
Amino Acids
Anemia, Diamond-Blackfan, 2
Animals
Blastocyst
Cytoplasm
Donors
Embryo
Females
Males
Mice, House
Mice, Inbred ICR
Mothers
Oviducts
RNA, Messenger
Strains
Tissue Donors
Uterus
Zygote
Most recents protocols related to «Uterus»
Example 20
Fertility—Progesterone is one of the most important hormones for pregnancy with myriad functions from ensuring implantation of the egg into a healthy uterine wall, to ensuring embryo survival and prevention of immune rejection of the developing baby. Many other hormones act in concert with progesterone, like Follicular Stimulating Hormone (FSH) and Luteinizing Hormone (LH) and can be used to assess optimal fertility windows on a monthly basis. And in fact an over dominant production of estrogen can lead to progesterone deficiency and thus difficulty getting or staying pregnant. It is important that women not only monitor FSH and LH to determine optimal fertility for getting pregnant, but ensure that sufficient levels or progesterone are being produced to ensure pregnancy and viability of the fetus. A study from the British Medical Journal, 2012, demonstrated that a single progesterone level test can help discriminate between viable and nonviable pregnancies. Among women who had an ultrasound, 73 percent had nonviable pregnancies. But among women with progesterone levels below 3 to 6 nanograms per milliliter, the probability of a nonviable pregnancy rose to more than 99 percent (Gallos L et al. British Medical J, 2012).
Perimenopause—Monitoring hormone levels during the menopausal transition may help women better understand important changes in their body and allow them to make more informed decisions about health, diet, and lifestyle. According to Hale G E (Best Pract Res Clin Obstet Gynaecol, 2009), data from endocrine studies on women throughout the menopausal transition show changes in levels of steroid hormones and gonadotropins (Progesterone, Estrodiol, LH, FSH and AMH) and follicle-stimulating hormone undergoes the first detectable change while menstrual cycles remain regular. Erratic and less predictable changes in steroid hormones follow, especially with the onset of irregular cycles. Later serum hormone studies on the inhibins and anti-Mullerian hormone established that diminishing ovarian follicle number contributes to the endocrine changes with advancing reproductive age.
Many fertility issues revolve around genetic, anatomical or other disorders that may either prevent a woman from becoming pregnant and/or staying pregnant. Some of these disorders include hormonal imbalances, diabetes, a short or insufficient cervix, and acute or chronic infections. A cascade of genes has been implicated in the occurrence of getting and staying pregnant. These genes have been studied using genotyping, gene expression, and proteomic analysis to assess a woman's ability to stay pregnant.
In some embodiments the disclosed device focuses on detecting levels of Progesterone, LH, FSH, Estrodiol, AMH, genotyping, gene expression through RNA and methylome sequencing, qPCR and proteomic analysis for fertility and menopause management from menstrual blood or cervicovaginal fluid.
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BLOOD
Cervix Uteri
Chronic Infection
Diabetes Mellitus
Diet
Embryo
Endocrine System Diseases
Epigenome
Estrogens
Fertility
Fetal Viability
Follicle-stimulating hormone
Gene Expression
Genes
Genes, vif
Gonadotropins
Hormones
Human Body
Human Follicle Stimulating Hormone
Infant
Inhibin
Luteinizing hormone
Medical Devices
Menopause
Menstrual Cycle
Menstruation
Mullerian-Inhibiting Hormone
Ovarian Follicle
Ovum Implantation
Perimenopause
Pregnancy
Progesterone
Reproduction
Steroids
System, Endocrine
Transcription, Genetic
Ultrasonography
Uterus
Woman
At the age of 70 weeks, a total of 120 hens were randomly selected to evaluate dietary interventions of body phosphorus rhythms. The hens were fed with 4 phosphorus regimens: (1) RR, provided with regular phosphorus diet at both 09:00 and 17:00 (conventional feeding without considering daily rhythms of body phosphorus metabolism); (2) RL, provided with regular phosphorus diet at 09:00 and low phosphorus diet at 17:00 (dynamic feeding converse to the body phosphorus rhythms found in Exp. 1); (3) LR, provided with low phosphorus diet at 09:00 and regular phosphorus diet at 17:00 (dynamic feeding consistent with the body phosphorus rhythms found in Exp. 1); (4) LL, provided with low phosphorus diet at both 09:00 and 17:00 (direct restriction without considering daily rhythms of body phosphorus metabolism). Each feeding regimens included 6 replicates, and each replicate contained 5 hens. The regular and the low phosphorus diet contained 0.32% and 0.14% NPP, respectively. The feeding trial lasted for 12 weeks (according to the literature, changes in eggshell and bone mineralization status could be observed in 8 to 12 weeks after dietary phosphorus interventions in laying hens) [26 (link), 27 (link)]. On the last 3 d of the feeding trial, all the eggs were collected for egg quality analysis. On the last day of the feeding trial, two egg-laying hens were randomly selected from each replicate (sampled at 6 and 18 h post-oviposition, respectively). The following samples were collected: blood (for serum), uterine (stored at −80 ℃, for Western-blotting analysis ), femur (left side, stored in 4% paraformaldehyde for histological analysis; right side, stored at −80 ℃ for the determination of mineralization status and gene expressions).
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BLOOD
Bone Mineralization
Dietary Modification
Dietary Phosphorus
DNA Replication
Egg Shell
Femur
Gene Expression
Human Body
Metabolism
Oviposition
paraform
Phosphorus
Physiologic Calcification
Serum
Therapy, Diet
Treatment Protocols
Uterus
Western Blot
Hy-Line Brown laying hens were fed with a regular diet (corn-soybean meal-based; containing 0.32% non-phytate phosphorus (NPP); Table 1 ) start from 35 weeks of age. On the last day of age 40 weeks, a total of 60 hens that laid eggs between 07:30−08:30 were randomly selected to evaluate the daily phosphorus rhythms. Of them, 45 hens were euthanized for sample collection, and the other 15 hens were used to study the feed intake and calcium/phosphorus excretion rhythms. For sample collection, the 45 hens were sampled according the oviposition cycle: at oviposition, at 6, 12, 18 h post-oviposition, and at the next oviposition, respectively, with 9 hens sampled at each of the time point. The following samples were collected: blood (for serum), uterine (stored at −80 ℃, for Western-blotting analysis), femur (in 4% paraformaldehyde, for histological analysis) and kidney (stored at −80 ℃, for Western-blotting analysis). For the other 15 hens, the feed intake was recoded and the excreta was collected at the following intervals: from oviposition to 6 h post-oviposition, from 7 to 12 h post-oviposition, from 13 to 18 h post-oviposition, from 19 h post-oviposition to the next oviposition.
Composition and nutrient concentrations of basal diet (%, unless noted, as-is basis)
| Item | Low phosphorus | Regular phosphorus |
|---|---|---|
| Ingredients | ||
| Corn | 56.69 | 56.69 |
| Soybean meal | 25.77 | 25.77 |
| Distillers dried grains with solubles | 4.00 | 4.00 |
| Calcium carbonate | 9.73 | 9.04 |
| Dicalcium phosphate | - | 1.15 |
| Soybean oil | 1.51 | 1.51 |
| Sodium chloride | 0.26 | 0.26 |
| DL-Methionine | 0.18 | 0.18 |
| Choline chloride | 0.15 | 0.15 |
| Montmorillonite | 0.71 | 0.25 |
| Premix1 | 1 | 1 |
| In total | 100.00 | 100.00 |
| Nutrient levels | ||
| Metabolizable energy, kcal/kg (calculated) | 2,600 | 2,600 |
| Crude protein (calculated) | 16.5 | 16.5 |
| Total phosphorus (calculated/analyzed) | 0.34/0.34 | 0.53/0.49 |
| Non-phytate phosphorus (calculated) | 0.14 | 0.32 |
| Calcium (calculated/analyzed) | 3.50/3.47 | 3.50/3.52 |
1Provided per kilogram of diet: manganese 60 mg, copper 8 mg, zinc 80 mg, iodine 0.35 mg, selenium 0.3 mg, vitamin A 8000 IU, vitamin E 30 mg, vitamin K3 1.5 mg, thiamine 4 mg, riboflavin 13 mg, pantothenic acid 15 mg, nicotinamide 20 mg, pyridoxine 6 mg, biotin 0.15 mg, folic acid 1.5 mg, and cobalamin 0.02 mg
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Biotin
BLOOD
Calcium, Dietary
calcium phosphate
Cereals
Choline
Copper
Corn Flour
Corns
Diet
Eggs
Feed Intake
Femur
Folic Acid
Iodine
Kidney
Manganese
Niacinamide
Nutrients
Oviposition
Pantothenic Acid
paraform
Phosphorus
Phytate
Proteins
Pyridoxine
Riboflavin
Selenium
Serum
Sodium
sodium phosphate
Soybean Flour
Soybeans
Specimen Collection
Thiamine
Uterus
Vitamin A
Vitamin B12
Vitamin E
Vitamin K3
Western Blot
Zinc-80
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Intestines
Patients
Radiotherapy, Intensity-Modulated
Rectum
Tissues
Urinary Bladder
Uterus
X-Ray Computed Tomography
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Acclimatization
Bladder Neoplasm
Cancer of Muscle
Cervix Uteri
Muscle Tissue
Nodes, Lymph
Parametrium
Patients
Pelvis
Radionuclide Imaging
Urinary Bladder
Uterus
Vagina
X-Ray Computed Tomography
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TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
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Fast Green is a laboratory staining dye used in various scientific applications. It is a synthetic, water-soluble dye that provides a green coloration. The core function of Fast Green is to stain and visualize specific components or structures within biological samples during microscopy and other analytical procedures.
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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M2 medium is a cell culture media formulation designed for the maintenance and cultivation of mouse embryos. It provides the necessary nutrients and growth factors to support the development and growth of mouse embryos in an in vitro environment.
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DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.
More about "Uterus"
The uterus is a remarkable and complex organ that plays a crucial role in the female reproductive system.
Also known as the womb, this muscular, hollow structure is responsible for the implantation and development of a fertilized egg, as well as the expulsion of the fetus during childbirth.
The uterus is composed of three main layers: the endometrium, the myometrium, and the perimetrium.
The endometrium is the inner lining of the uterus that undergoes cyclic changes during the menstrual cycle.
This dynamic process is essential for the preparation of the uterus to receive a fertilized egg.
The myometrium, the middle layer, is the muscular component that contracts during childbirth, facilitating the delivery of the fetus.
The perimetrium is the outer, serous layer that covers the uterus.
The uterus is connected to the fallopian tubes, which transport the egg from the ovaries to the uterus.
This intricate network allows for the seamless passage of the egg and the potential for fertilization.
Proper understanding and optimization of uterine function are crucial for women's health and reproductive outcomes.
Researchers often utilize various techniques and tools to study the uterus and its function.
TRIzol reagent, a popular RNA extraction method, can be used to isolate high-quality RNA from uterine tissue samples.
Fast Green, a staining dye, can be employed to visualize specific cellular structures within the uterus.
Fetal bovine serum (FBS) is a commonly used supplement in cell culture media, such as DMEM/F12, to support the growth and maintenance of uterine cells in vitro.
The RNeasy Mini Kit, another RNA extraction method, can be used to purify RNA from uterine samples for downstream analysis.
Penicillin/streptomycin, an antibiotic mixture, is often added to cell culture media to prevent bacterial contamination during uterine cell experiments.
RNAlater, a RNA stabilization solution, can be used to preserve uterine tissue samples for later analysis.
The High-Capacity cDNA Reverse Transcription Kit is a valuable tool for converting extracted RNA into complementary DNA (cDNA), which can then be used for gene expression studies related to uterine function.
By leveraging these research tools and techniques, scientists can gain deeper insights into the complex mechanisms underlying uterine physiology and pathology, ultimately leading to advancements in women's health and reproductive medicine.
Also known as the womb, this muscular, hollow structure is responsible for the implantation and development of a fertilized egg, as well as the expulsion of the fetus during childbirth.
The uterus is composed of three main layers: the endometrium, the myometrium, and the perimetrium.
The endometrium is the inner lining of the uterus that undergoes cyclic changes during the menstrual cycle.
This dynamic process is essential for the preparation of the uterus to receive a fertilized egg.
The myometrium, the middle layer, is the muscular component that contracts during childbirth, facilitating the delivery of the fetus.
The perimetrium is the outer, serous layer that covers the uterus.
The uterus is connected to the fallopian tubes, which transport the egg from the ovaries to the uterus.
This intricate network allows for the seamless passage of the egg and the potential for fertilization.
Proper understanding and optimization of uterine function are crucial for women's health and reproductive outcomes.
Researchers often utilize various techniques and tools to study the uterus and its function.
TRIzol reagent, a popular RNA extraction method, can be used to isolate high-quality RNA from uterine tissue samples.
Fast Green, a staining dye, can be employed to visualize specific cellular structures within the uterus.
Fetal bovine serum (FBS) is a commonly used supplement in cell culture media, such as DMEM/F12, to support the growth and maintenance of uterine cells in vitro.
The RNeasy Mini Kit, another RNA extraction method, can be used to purify RNA from uterine samples for downstream analysis.
Penicillin/streptomycin, an antibiotic mixture, is often added to cell culture media to prevent bacterial contamination during uterine cell experiments.
RNAlater, a RNA stabilization solution, can be used to preserve uterine tissue samples for later analysis.
The High-Capacity cDNA Reverse Transcription Kit is a valuable tool for converting extracted RNA into complementary DNA (cDNA), which can then be used for gene expression studies related to uterine function.
By leveraging these research tools and techniques, scientists can gain deeper insights into the complex mechanisms underlying uterine physiology and pathology, ultimately leading to advancements in women's health and reproductive medicine.