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Pulp Canals

Pulp Canals, also known as root canals, are the hollow, central cavities within a tooth that contain the dental pulp.
The pulp is composed of soft connective tissue, blood vessels, and nerves that nourish and innervate the tooth.
Proper identification and treatment of the pulp canal is crucial for maintaining dental health, as infection or inflammation of the pulp (pulpitis) can lead to tooth loss if left untreated.
Dentists and endodontists utilize specialized techniques and procedures to access, clean, and seal the pulp canal, preserving the natural tooth structure.
Reasearching best practices and protocols for pulp canal treatment can help optimize outcomes and improve patient care.
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Most cited protocols related to «Pulp Canals»

Since MD trajectories now typically produce tens of thousands of conformational snapshots it is necessary to be able to analyse such large datasets quickly. Curves+ can read MD trajectory files directly without the need for creating PDB format files. It currently deals with AMBER format trajectory files. It could be adapted to other formats, but it is also relatively easy to modify trajectory files to the AMBER format (see, for example, Simulaid by M. Mezei http://atlas.physbio.mssm.edu/∼mezei/simulaid/or CatDCD by J. Gullingsrud http://www.ks.uiuc.edu/Development/MDTools/catdcd/).
Curves+ can be used to pick out and analyse a single snapshot or to analyse snapshots at chosen time intervals for the whole trajectory or extract information on a given sequence fragment from an ensemble of trajectories. The present version of Curves+ can analyse roughly 100 conformational snapshots of a 20-bp double-stranded DNA oligomer per second on a 2.5 GHz processor. When multiple snapshots are treated, printed output is suppressed and the program creates an unformatted file which can be treated with a supplementary program named Canal (Curves+ analysis). This program calculates the maxima, minima, mean and standard deviations of any chosen conformational parameters which are output in a list file. It can also generate time series and histograms which are output in flat files that can be used for producing graphics and will optionally calculate linear correlation coefficients between all helical, backbone and groove parameters, which can again be output in files for checking correlations graphically. The simple format of all files output by Canal makes them useable in any common graphic program. We have used both Gnuplot and MatLab (Mathworks Inc.) in preparing the illustrations of Canal data for this article. Canal can lastly be applied to the analysis of files produced by Curves+ from single structures. In this case, it can be used to plot the variation of chosen parameters along an oligomer or, as with trajectories, to look at parameter distributions and correlations. The use of Canal will be further illustrated in an article (manuscript in preparation) concerning the analysis of multiple MD trajectories from the ABC dataset (40 (link),41 (link)). A full user guide for Canal is available at the web site cited below.
Publication 2009
Amber DNA Conformation Helix (Snails) Pulp Canals Toxic Epidermal Necrolysis Vertebral Column
Mice were anesthetized via inhalation isoflurane (2%). The surgical procedure was modified from previous work [49] (link), [50] (link). A skin incision was made over the right knee (Figure 1A). The distal right femur was accessed through a medial parapatellar arthrotomy with lateral displacement of the quadriceps-patellar complex (Figure 1B). After locating the femoral intercondylar notch (Figure 1B), the femoral intramedullary canal was manually reamed with a 25 gauge needle (Figure 1C). An orthopaedic-grade stainless steel Kirschner (K)-wire (diameter 0.6 mm) (Synthes) was surgically placed in a retrograde fashion and cut with 1 mm protruding into the joint space (Figure 1D). An inoculum of S. aureus in 2 µl of normal saline was pipetted into the joint space containing the cut end of the implant (Figure 1E). The quadriceps-patellar complex was reduced to the midline (Figure 1F) and the surgical site was closed with Dexon 5-0 sutures (Figure 1G). A representative radiograph demonstrates the position of the implant with good intramedually fixation of the stem and prominence of the cut surface in the joint (Figure 1H). Buprenorphine (0.1 mg/kg) was administered subcutaneously every 12 hours as an analgesic for the duration of the experiment.
Publication 2010
Analgesics Buprenorphine dexon (fungicide) Femur Inhalation Isoflurane Joints Kirschner Wires Knee Joint Mus Needles Normal Saline Operative Surgical Procedures Patella Pulp Canals Quadriceps Femoris Skin Stainless Steel Stem, Plant Sutures X-Rays, Diagnostic
Prior to any gain calculations, each individual head and eye velocity record was checked to ensure that an acceptable head impulse movement was achieved, free of any artifact (12 (link)). In the head velocity component, this meant no head movement prior to the impulse, and as abrupt a stop as possible with a maximum “bounce-back” velocity of <25% of peak head velocity. For the eye velocity, it meant no eye movement prior to the onset of the head movement, and no obvious goggle movement or eyelid artifacts. Any impulse with these artifacts was eliminated prior to gain calculations. The remaining average number of impulses in each direction after removing the traces with artifacts ranged from a low of 32 impulses per subject (right anterior, 50–59 age group) to a maximum of 57 impulses per subject (right horizontal, 80–89 age group).
Vestibulo-ocular reflex gain was calculated as follows. The time of peak onset head acceleration was determined for each impulse (2 (link)), and head impulse onset was defined as occurring 60 ms before this time. Head impulse offset was defined as the moment when head velocity crossed zero velocity again (1 (link), 3 (link)). Following the methods previously described (1 (link), 3 (link)), the eye velocity time series were first desaccaded: saccades were identified by an eye acceleration criterion, and linear interpolation was used to replace the removed saccade. Then, the area under the desaccaded eye velocity curve from the start to the end of the head impulse was calculated and compared to the area under the head velocity curve during the same interval. VOR gain was defined as the ratio of these two areas. This is a position gain rather than the traditional slope gain (velocity) calculation, because our measurements and simulations (3 (link)) have shown that this method of calculating VOR gain with vHIT is more resistant to artifact – due, e.g., to slippage of the goggles – than the instantaneous VOR gain calculations using velocity. It is also a more functional measure of vestibulo-ocular performance, as it is the eye position error at the end of the head impulse (how far fixation is from the fixation target), which is the driver for the corrective saccade in the case of vestibular loss. VOR position gain and the corrective saccades are complementary (5 (link)).
For each subject, the VOR gain of each impulse was plotted as a function of peak head velocity (Figure 2A). A line of best fit [using the lowess procedure (13 ) to perform a robust locally weighted regression] was fitted to those values using a smoothing fraction f, which depended on the range in head velocity covered, being chosen to correspond to an interval of 50°/s in peak head velocity. A cubic spline interpolation (using natural splines, where the second derivative was equal to zero at the endpoints) applied to the lowess-fitted data then provided a vector of VOR gain values at each 0.2°/s increment of peak head velocity (Figure 2A). The VOR gain vectors across all the subjects in that decade age group (Figure 2B) were averaged to form a vector of average VOR gain as a function of peak head velocity for that decade age group together with a band of ±95% confidence intervals of the mean (Figure 2C) and a band of the mean ± 2 SDs (Figure 2D) to show the band across velocities in which the results of 95% of the healthy population in that decade age group are expected to fall (8 ). This data processing procedure was repeated for each decade band for horizontal, anterior, and posterior canals. Plots of two-tailed 95% confidence intervals were important for showing whether the VOR data for an age band included the VOR gain of 1.0.
Publication 2015
Acceleration Age Groups Cloning Vectors Cuboid Bone Eyelids Eye Movements Functional Performance Head Head Movements Movement Population Health Pulp Canals Vestibular Labyrinth Vision
The MEDLINE (1966–2010), EMBASE (1980–2010), and CINAHL (1982–2010) databases were searched for English-language diagnostic accuracy studies of the clinical syndrome of LSS in adults (eMethods available at www.jama.com). Two reviewers (P.S. and L.K.) reviewed all abstracts to assess adherence to review criteria. Inclusion criteria consisted of the following: (1) diagnostic accuracy study of the history, physical examination, or both for the diagnosis of the clinical syndrome of LSS, with or without spondylolisthesis (a displacement of 1 vertebra atop another); (2) reporting of sensitivity, specificity, accuracy, predictive values, likelihood ratios, or prevalence in cases and controls; (3) index tests that were either clearly specified or described or that were used in common practice and could be performed in a routine clinic visit without specialized equipment; and (4) use of an appropriate reference standard that was clearly specified or described. Studies were excluded if they consisted of a mixed population including stenosis in nonlumbar areas or red flag conditions31 (link) (ie, trauma, infection, or malignancy), if they included only patients with scoliosis or congenital stenosis, or if they consisted of case series. We determined quality using levels of evidence for the Rational Clinical Examination.32 (link)The gold standard for diagnosis of the clinical syndrome of LSS is the impression of an expert clinician, with radiographic or anatomic corroboration of spinal canal narrowing. The expert clinician impression is a common reference standard in musculoskeletal medicine because the production of pain cannot be assessed by a single laboratory or imaging test.33 (link) We required expert opinion based on a combination of clinical assessment and imaging evaluation by computed tomography (CT), MRI, or myelography, or expert opinion based on a combination of clinical assessment and a clearly defined, prospectively established protocol for intraoperative evaluation. Simple surgical confirmation or verification of a prior diagnosis without clearly stated pre hoc criteria for inclusion and exclusion was considered insufficient as an intraoperative diagnosis.
Sensitivities and specificities were calculated from the raw data where presented, and contingency tables were created using reported prevalence, sensitivities, and specificities where raw data were not available. Likelihood ratios (LRs) for the diagnosis of the clinical syndrome of LSS were calculated for positive test results [LR+ = (sensitivity/(1 − specificity)] and negative test results [LR− =(1 − sensitivity)/specificity]. We calculated 95% confidence intervals (CIs) according to the method of Simel et al.34 (link) We used Excel 2007 (Microsoft, Redmond, Washington) for statistical analyses and checked these values using SAS version 9.2 (SAS Institute Inc, Cary, North Carolina).
Publication 2010
Adult Clinic Visits Diagnosis Gold Hypersensitivity Infection Malignant Neoplasms Myelography Operative Surgical Procedures Pain Patients Pharmaceutical Preparations Physical Examination Pulp Canals Scoliosis Spondylolisthesis Stenosis Syndrome Tests, Diagnostic Vertebra Wounds and Injuries X-Rays, Diagnostic
Backbone parameters comprise the single bond torsions along the phosphodiester chain and the conformation of the sugar ring. In a conventional DNA strand, the backbone segment associated with each nucleotide (in the 5′→3′ direction) is described by the torsions α (03′-P-O5′-C5′), β (P-O5′-C5′-C4′), γ (O5′-C5′-C4′-C3′), δ (C5′-C4′-C3′-O3′), ϵ (C4′-C3′-O3′-P) and ζ (C3′-O3′-P-O5′), to which we must add the glycosidic angle χ (O4′-C1′-N1-C2 for pyrimidines and O4′-C1′-N9-C4 for purines) joining the sugar to the base and the ribose OH torsion (C1′-C2′-O2′-H2′) in the case of RNA.
We remark that calculating averages and standard deviations of angular variables is not trivial, unless they cover restricted angular ranges. There is also no simple definition of maximal and minimal values. This problem occurs in many branches of science with broadly distributed angular variables, for example, in analysing wind directions (30 ). While angular helical variables generally lie within limited ranges, backbone dihedrals can easily span the full range of 360°. In this case, maximal and minimal values in the Curves+ analysis are replaced with the parameter ‘range’ and angular averages and standard deviations are calculated using a vectorial approach. Range is defined as the number of 1° bins visited by a given variable in the interval 0–360°. This gives a good idea of the angular spread of variables. Note that when analysing molecular dynamics trajectories, this value may increase with sampling, giving an indication that more sampling probably needs to be done. However, the details of the angular distribution can be checked using the histogram output option of the supplementary program Canal (see below). For averages, angles are added as vectors in 2D space (with an angle θ having components x = Cos θ and y = Sin θ). The result is converted to a unit vector, whose X and Y components yield the average. Other approaches require assuming that the angles obey a presupposed type of distribution. We have checked our values against one such model (31 ), and found negligible differences for standard deviations up to roughly 20°. Larger values differ more significantly (5–10°), but in these cases it is the qualitative result that the variables in question fluctuate very strongly that is the most important.
The sugar ring is usefully described using pseudorotation parameters. Although strictly speaking there are four pseudorotation parameters for a five-membered ring (32 (link)), only two of these, the so-called phase (Pha) and amplitude (Amp), are generally useful. While the amplitude describes the degree of ring puckering, the phase describes which atoms are most displaced from the mean ring plane. We calculate these parameters using the formulae given below (33 ), which have the advantage of treating the ring dihedrals ν1 (C1′-C2′-C3′-C4′) to ν5 (O4′-C1′-C2′-C3′) in an equivalent manner. In this approach:

where and b =−0.4 note, if then .
Conventionally, sugar ring puckers are divided into 10 families described by the atom which is most displaced from the mean ring plane (C1′, C2′, C3′, C4′ or O4′) and the direction of this displacement (endo for displacements on the side of the C5′ atom and exo for displacements on the other side). These pucker families can be easily calculated from the phase angle and are also output by the Curves+ program.
In order to deal with non-standard nucleic acids the backbone parameters are not hard-wired into the program, but are contained in a data file (standard_s.lib) which can be modified or extended by this user. This makes it easy to analyse chemically modified backbones such as those, for example, in PNA (34 (link)).
Publication 2009
Carbohydrates Cloning Vectors Displacement, Psychology Endometriosis Glycosides Helix (Snails) Molecular Dynamics Nucleic Acids Nucleotides Pulp Canals purine Pyrimidines Ribose single bond Vertebral Column Wind

Most recents protocols related to «Pulp Canals»

All surgeries were performed by a single surgeon (WFK), using a posterolateral approach to the hip. The proximal femur was serially broached in 2.5 mm increments until a press fit was achieved. The rasping of the femoral canal was performed to match the native version of the proximal femur. The femoral component used was the same size as the last rasp. Antibiotics were administered preoperatively and continued for 48 hours following surgery. Anticoagulation consisted of warfarin administered orally the day of surgery, and continued for 30 days following surgery.
Publication 2023
Antibiotics, Antitubercular Femur Operative Surgical Procedures Pulp Canals Surgeons Surgery, Day Warfarin
Following the institutional review board approval for the study (number: 119/2019; Muğla Sıtkı Koçman University Ethical Committee), a retrospective cohort analysis was performed using the medical records of patients. For the current study, patient consent is not required. All procedures executed involving human participants were in accordance with the ethical standards of the institutional ethical committee and with the 1964 Helsinki declaration.
A total of 146 patients who applied to the neurosurgery outpatient clinic with a recent abdominal CT (max three months) because of a lower back pain complaint were included in the study. Patients with a previous history of surgery or a vertebral fracture were excluded. After excluded patients, a total of 146 patients were included in the study, of whom 90 were female (61.6%) and 56 were male (38.4%). The mean age of the patients was 51.42±13.91 (20-82) years.
Lumbar vertebra CT scans of all patients were reviewed retrospectively. CT images at the level from L3-L4 intervertebral disc were analyzed for body composition of fat tissue and muscle mass volume through the dedicated CT software (Syngo.via, SOMATOM Definition Flash: Siemens Healthcare, Forchheim, Germany). The L3-L4 level was selected in sagittal reformat CT images with the software (Figure 1).
The density range of -200, -40 HU was selected for the fat density measurement in the cross-section with the "region grooving" application in the angled axial images obtained parallel to the disc plane at this level. First, the fat volume in the whole section was measured (visceral and subcutaneous). Then, only the visceral adipose tissue volume was calculated by drawing borders to exclude subcutaneous adipose tissue (Figure 2). The subcutaneous fat tissue volume was obtained by subtracting the visceral fat tissue volume from the total fat volume (Figure 3).
With the same application, muscle density was selected and paravertebral muscle tissue volume was calculated (bilateral musculus psoas major, musculus quadratus lumborum, musculus iliocostalis, musculus longissimus, musculus multifidus volumes). A Spearman correlation model was used to analyze visceral adiposity, subcutaneous fat, and muscle mass.
In CT images, each intervertebral disc space was evaluated in terms of the presence of osteophytes, loss of disc height, sclerosis in the end plates, and spinal stenosis (spinal canal narrowing under 15 mm AP diameter) to investigate the presence of degeneration. Each level was scored according to the presence of findings, with 1 point for the presence of osteophytes, loss of disc height, sclerosis in the end plates, and spinal stenosis. The total score at all levels (L1-S1) was calculated for each patient.
Statistical analyses were performed using IBM SPSS version 20.0 software (IBM Corp., Armonk, NY). The conformity of the data to normal distribution was assessed using the Shapiro-Wilk test. Normally distributed variables were presented as mean±standard deviation and those not showing normal distribution as median (minimum-maximum) values. Categorical variables were presented as numbers (n) and percentages (%). The Spearman's rank correlation coefficient test was used to determine the correlation between the measured parameters in various vertebral pathologies. Continuous variables were compared using the Mann-Whitney U test. The receiver operating characteristic (ROC) analysis was used to detect the area under the curve (AUC) and define the cutoff values with their sensitivities and specificities of the measurements. An alpha value of p<0.05 was accepted as statistically significant.
Publication 2023
Abdomen Cone-Beam Computed Tomography Ethics Committees, Research Homo sapiens Intervertebral Disc Low Back Pain Males Multifidus Muscle Tissue Neurosurgical Procedures Obesity, Visceral Operative Surgical Procedures Osteophyte Patients Psoas Muscles Pulp Canals Sclerosis Spinal Fractures Spinal Stenosis Subcutaneous Fat Vertebra Vertebrae, Lumbar Visceral Fat Woman X-Ray Computed Tomography
In Yuan et al study,[29 (link)] patients received standardized general anesthesia and basic analgesic protocol. Intraoperatively, all patients received general anesthesia which was induced by sufentanil 0.5 μg/kg, midazolam 0.04 mg/kg, propofol 1 to 2 mg/kg, and Cisatracurium 2 μg/kg intravenously, followed by continuous intravenous infusion of remifentanil 0.1 to 0.3 μg/(kg·min), propofol 2 to 5 mg/(kg·hr) and inhalation of sevoflurane to maintain anesthesia. Since postoperative day 1, the protocol of oral celecoxib restarted till postoperative 3 weeks when the patients came back to the hospital for taking out the stitches. In Yadeau et al 2016 study,[6 (link)] patients received a standardized anesthetic and multimodal analgesic protocol. In Yadeau et al 2022 study,[28 (link)] patients received a standard intraoperative and postoperative multimodal anesthetic protocol: a spinal-epidural (subarachnoid mepivacaine, 45–60 mg); adductor canal block (ultrasound-guided; 15 cc bupivacaine, 0.25%, with 2 mg preservative-free dexamethasone). For postoperative pain management, patients were scheduled to receive the study medication once daily for 14 days; 4 doses of 1000 mg IV acetaminophen every 6 hours followed by 1000 mg oral acetaminophen every 8 hours; 4 doses of 15 mg IV ketorolac followed by 15 mg meloxicam every 24 hours; and 5 to 10 mg oral oxycodone was given as needed for pain. Patients could have pain medications adjusted as indicated. In Koh et al study,[12 (link)] all patients had a postoperative intravenous patient-controlled anesthesia (PCA) pump that administered 1 mL of a 100-mL mixture containing 2000 mg of fentanyl on demand. In Kim et al study,[27 ] all patients received intravenous PCA encompassing delivery of 1 mL of a 100 mL solution containing 2000 µg of fentanyl postoperatively. In Ho et al study,[26 (link)] patients were routinely offered a single shot spinal anesthesia consisting of an intrathecal dose of bupivacaine 10 to 12.5 mg with fentanyl 10 mg. After surgery, pain treatment consisted of PCA with intravenous injection of morphine. The settings were 1 mg bolus, 5 minutes lockout time, and a maximum hourly limit of 8 mg. All patients were also given acetaminophen 1 g 6 hourly.
Publication 2023
Acetaminophen Analgesics Anesthesia Anesthesia, Intravenous Anesthetics Bupivacaine Cardiac Arrest Celecoxib cisatracurium Dexamethasone Fentanyl General Anesthesia Inhalation Intravenous Infusion Ketorolac Management, Pain Meloxicam Mepivacaine Midazolam Morphine Multimodal Imaging Obstetric Delivery Operative Surgical Procedures Oxycodone Pain Pain, Postoperative Patients Pharmaceutical Preparations Pharmaceutical Preservatives Propofol Pulp Canals Remifentanil Sevoflurane Spinal Anesthesia Subarachnoid Space Sufentanil Ultrasonography
Another subgroup of SHR-S, SHR-T, Wistar-S, and Wistar-T received, after the functional measurements, an overdose of ketamine + xylazine. Immediately after the respiratory arrest, the thorax was opened and the left ventricle cannulated for sterile saline perfusion (∼30 mL/min, Daigger pump, Vernon Hills IL United States) followed by modified Karnovsky solution (2.5% glutaraldehyde +2% paraformaldehyde in 0.1 M PBS, pH 7.3). Brain was removed and placed on a coronal brain matrix (72–5029, Harvard Apparatus) to obtain hypothalamic and brainstem slices. PVN, NTS, and RVLM nuclei were microdissected with the aid of a magnifying lens, using as anatomic markers the third ventricle and optic chiasma, the central canal and 4th ventricle, and, the nucleus ambiguous, raphe obscurus and inferior olive, respectively. The nuclei were immersed in a 2.5% glutaraldehyde solution for 2 h, washed in PBS and post-fixed in a 2% osmium tetroxide solution for 2 h at 4°C. Tissues were then stained overnight with uranyl acetate, dehydrated in 60% up to 100% ethanol series and immersed in pure resin. Semi-thin slices (400 nm, ultra-microtome Leica EMUC6) were obtained, placed in glass slides and stained with Toluidine Blue in order to select adequate areas for further processing. Ultra-thin slices (60 nm) were obtained with diamond knife, contrasted with 4% uranyl acetate and 0.4% lead acetate and disposed in 200 copper mesh screens.
Transverse sections of PVN, NTS, and RVLM capillaries of the 4 experimental groups were acquired in a transmission electron microscope (FEI Tecnai G20, 200 KV) and analyzed by a blind observer using the ImageJ software. The following parameters were analyzed in 9–11 capillaries/area/rat, 3 rats/experimental group: luminal and abluminal perimeter, lumen diameter, area of the endothelial cell, thickness of the basement membrane, pericytes’ coverage of capillaries, extension of capillary border between adjacent endothelial cells, the occurrence/extension of tight junctions, and, the counting of transcellular vesicles/capillary. To avoid the inclusion of non-transcytotic vesicles such as lysosomes, endosomes, peroxisomes, only the vesicles being formed at the luminal, and abluminal membranes were counted. Vesicle counting was expressed as number/capillary. Using the zoom to expand acquired images, the whole extension of capillaries was analyzed.
Publication 2023
Brain Brain Stem Capillaries Cell Nucleus Chest Copper Diamond Drug Overdose Endosomes Endothelial Cells Ethanol Glutaral Hypothalamus Ketamine lead acetate Left Ventricles Lens, Crystalline Lysosomes Membrane, Basement Microtomy Nucleus Raphe Obscurus Olivary Nucleus Optic Chiasms Osmium Tetroxide paraform Perfusion Pericytes Perimetry Peroxisome Phenobarbital Pulp Canals Rattus norvegicus Resins, Plant Respiratory Rate Saline Solution Sterility, Reproductive Tight Junctions Tissue, Membrane Tissues Tolonium Chloride Transcytosis Transmission Electron Microscopy uranyl acetate Ventricles, Fourth Ventricles, Third Visually Impaired Persons Xylazine

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Publication 2023
Bones Cancellous Bone Epiphyseal Cartilage Femur Medulla Oblongata Osteopenia Pulp Canals Radiography Radionuclide Imaging X-Ray Microtomography

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