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Body Fluids

Body fluids refer to the various aqueous solutions and secretions found within the human body, including blood, urine, cerebrospinal fluid, synovial fluid, and others.
These fluids serve critical physiological functions, such as transporting nutrients, facilitating waste removal, and enabling proper organ and tissue function.
Analyzing the composition and characteristics of body fluids can provide valuable insights into an individual's health status and assist in the diagnosis and monitoring of various medical conditions.
Researchers often study body fluids to better understand the underlying mechanisms of disease, develop new diagnostic tools, and explore potential therapeutic interventions.
This area of study is crucial for advancing medical knowledge and improving patient outcomes.

Most cited protocols related to «Body Fluids»

For EHR data (UPMC, KPNC, and VA), the first episode of suspected infection was identified as the combination of antibiotics (oral or parenteral) and body fluid cultures (blood, urine, cerebrospinal fluid, etc). We required the combination of culture and antibiotic start time to occur within a specific time epoch. If the antibiotic was given first, the culture sampling must have been obtained within 24 hours. If the culture sampling was first, the antibiotic must have been ordered within 72 hours. The “onset” of infection was defined as the time at which the first of these 2 events occurred (eAppendix in the Supplement). For non-EHR data in ALERTS, patients were included who met US Centers for Disease Control and Prevention definitions or clinical criteria for hospital-acquired infection more than 48 hours after admission as documented by prospective screening.7 (link) For non-EHR data in KCEMS, administrative claims identified infection present on admission (Angus implementation of infection using International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) diagnosis codes).6 (link)
Publication 2016
Antibiotics Blood Body Fluids Cerebrospinal Fluid Diagnosis Dietary Supplements EPOCH protocol Infection Infections, Hospital Parenteral Nutrition Patients Urine
We performed quantitative real-time PCR (qPCR) according to manufacturer’s instructions (Qiagen) to profile the miRNA distribution in body fluid samples. In brief, 5 μL total RNA was collected and pooled from the samples from the same fluid type, and the cDNA was produced using the miScript Reverse Transcription kit (Qiagen). We used the Matrix Hydra eDrop (Thermo Scientific) to mix the cDNA sample and the qPCR master reagent [Human miScript Assay 384 set v10.1 (Qiagen)] to reduce pipetting error. Any wells with multiple melting-temperature values were excluded from further analysis. We also used individual Human miScript Assays to validate the 384 miRNA qPCR set. Data were analyzed using SDS Enterprise Database 2.3 (Applied Biosystems) and normalized with global mean instead of specific miRNA or noncoding RNA signals.
Publication 2010
Biological Assay Body Fluids DNA, Complementary Homo sapiens Hydra MicroRNAs Real-Time Polymerase Chain Reaction Reverse Transcription RNA, Untranslated
In addition to the 44 tissue samples from the degradation and reproducibility analysis, the 16 individual lung cancer tissues and the 61 tissues from two bodies newly measured for this study, we searched the literature for other studies where normal tissues have been profiled. In the GEO (9 (link)), we found 1178 series related to miRNAs. Of these, 722 were from Homo sapiens. Excluding series with low sample count (below 20 samples), 302 series remained. After excluding studies from body fluids such as serum, plasma, blood or urine, we examined the remaining hits for availability of unaffected tissue measurements. The respective data tables were downloaded from GEO and all IDs were matched from the respective platform identifiers to miRBase Version 21 IDs. For the respective studies, raw and normalized data (VSN and quantile normalized) were added to our tissue atlas web repository. These include 43 samples from 9 tissues and 463 miRNAs from GSE11879, 40 samples measured for 709 miRNAs from normal gastric tissue from GSE23739, 48 benign prostate tissues measured for 480 miRNAs from series GSE54516 and 32 benign prostate tissues measured for 825 miRNAs from series GSE76260. The data have been used partially in the present manuscript, all data are included in the web-based tissue atlas resource.
Publication 2016
BLOOD Body Fluids Homo sapiens Lung Cancer MicroRNAs Plasma Prostate Serum Stomach Tissues Urine
In our study, we precisely replicated the Sepsis-3 task force [3 (link), 4 (link)] definition of suspected infection as the acquisition of a body fluid culture temporally contiguous to administration of antibiotics. Other data extracted included patient demographics and all necessary variables for calculating SOFA scores [11 (link)], which were calculated using data from the first 24 hours of the ICU stay. The sepsis-3 criteria for sepsis were extracted as suspected infection with associated organ dysfunction (SOFA≥2). Five other definitions of sepsis were extracted: (1) explicit criteria: the presence of at least one of the two proposed International Classification of Diseases 9th Revision (ICD-9) codes explicitly mentioning sepsis (995.92, severe sepsis and 785.52, septic shock); (2) Angus methodology: ICD-9 codes for sepsis as proposed by Angus et al. [8 (link)]; (3) Martin methodology: ICD-9 codes proposed by Martin et al. [9 (link)]; (4) the Centers for Medicare & Medicaid Services (CMS) criteria: an adaptation of the CMS Severe Sepsis and Septic Shock Management Bundle (NQF #0500) which uses a combination of diagnostic ICD-9 codes, SIRS criteria, and specific thresholds for organ dysfunction [12 ]; and (5) the Center for Disease Control (CDC) complete surveillance criteria, which utilize suspicion of infection criteria that are identical to Sepsis-3 along with organ dysfunction criteria that are similar (but not identical) to SOFA [13 (link)].
Publication 2017
Acclimatization Antibiotics Body Fluids Diagnosis Infection Patients Septicemia Septic Shock Severe Sepsis Systemic Inflammatory Response Syndrome
Protein expression sources provided data either via txt files (PaxDb version 3.0 integrated datasets, MOPED version 2.5, MaxQB 09/2013) or via API methods (ProteomicsDB 02/2015). A standard symbolization algorithm was developed to map all identifiers used [sources gene identifiers, Ensembl (24 (link)) and UniProt (25 (link)) protein identifiers] to gene symbols, using GeneCards aliases, identifiers and protein annotations. For genes having multiple proteins, expression data were summed to receive a gene centric aggregated value. For proteins belonging to multiple genes, protein intensity values were equally divided among such genes.
For integration, we converted all abundance data to mole-based parts per million (PPM), whereby for MaxQB and ProteomicsDB, iBAQ (intensity based absolute quantification) was converted to PPM by
PPMi=iBAQijiBAQj·106
Duplicate samples and samples having <10 mapped genes were excluded from HIPED. This resulted in the definition of 771 proteomic samples (Supplementary Table S1). Of these, 555 samples represented cell lines and disease-related datasets, and 216 samples were normal human anatomical entities (tissues, in vivo cells and body fluids). Experimental data of individual samples independently curated by different sources were pre-averaged (Supplementary Table S4). Further averaging across data sources (see Supplementary Table S2 and legend), we ended up with 69 datasets for normal anatomical entities (Supplementary Table S3) and 125 for the cell lines (Supplementary Table S1). Averages were calculated as geometric mean of non-zero PPM values. Three datasets with extremely high gene counts (>12 000; Supplementary Figure S5 outliers, proteomes #15, 17, 18 in Supplementary Table S2) were excluded from averaging.
Publication 2016
Body Fluids Cell Lines Cells Genes Homo sapiens link protein Multiple Birth Offspring Nevus Protein Annotation Proteins Proteome Tissues

Most recents protocols related to «Body Fluids»

Example 4

To evaluate the ability of hydroxyapatite formation by the bioceramic compositions, samples with a mean particle size of 1.5 μm were stored in a solution of simulated body fluid (SBF) pH 7.25 at 37° C. and evaluated at 1, 3, 5, 7, 10 and 20 days using the mass/volume ratio of 1.5 mg/mL. After 20 days the disks were washed with water and dried at 60° C. The amount of hydroxyapatite was determined by the phosphorus (P) content in the samples by dispersive energy X-ray fluorescence spectrometry. The mass percentage found is related to hydroxyapatite formation. The results are presented in Table 5.

TABLE 5
Percentages by weight of phosphorus obtained by area mapping
by X-ray fluorescence of the bioceramic compositions 1 to 6.
Days
Element P (%)
Samples12351020
CB 10.0810.0750.0800.1310.2030.213
CB 20.0620.0710.0750.0820.1010.123
CB 30.0550.0510.0630.0790.0880.095
CB 40.0790.0850.0940.0990.1250.182
CB 50.0820.0900.1050.1220.1430.196
CB 60.0950.1050.1320.1600.1840.208

While some embodiments are shown and described herein, one skilled in the art will appreciate that modifications and variations are possible in light of the above teachings.

Patent 2024
Biological Assay Body Fluids CB 184 Durapatite Fluorescence Light Phosphorus Roentgen Rays Spectrometry, X-Ray Emission Teaching
Not available on PMC !

Example 4

FIG. 1 shows the N2 adsorption isotherm of sample 2.

Bioactivity testing (ability to precipitate hydroxyapatite) was carried out on sample 2 using a simulated body fluid test.

FIG. 2 shows the absorbance spectra after 3 hours of immersion in simulated body fluid. The precipitation of hydroxyapatite is confirmed by the presence of two bands at 560 and 600 cm−1.

This is an industry standard test to demonstrate that a material is bioactive. This test is widely accepted to demonstrate that a material which is bioactive in simulated body fluid would, once in the body, be able to form bone on its surface. This is an essential property for bone substitute materials.

FIGS. 3 and 4 show a scanning electron micrograph of sample 2 after calcination.

The structure of the unreacted sample 2 shows silica spheres forming a bioactive aerogel structure.

This data demonstrates that the bone graft substitutes of the present invention are bioactive and exhibit low densities and high surface areas, compared to typically used bones graft substitutes.

Patent 2024
Adsorption Body Fluids Bones Bone Substitutes Bone Transplantation Durapatite Electrons Figs Grafts Human Body Silicon Dioxide Submersion

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Publication 2023
Biological Transport, Active Body Fluids Fluorescence Silicon Dioxide Sodium Fluorescein Syringes Ultraviolet Rays
DNA was isolated from thawed plasma samples using the TIANamp Blood DNA Kit (Tiagen Biotech, Beijing, China) according to the manufacturer’s instructions for blood and body fluids protocol. Prior to DNA isolation, plasma samples were centrifuged at 10 000 g for 10 min. Quantitative analysis of cell-free mtDNA was performed using quantitative real time polymerase chain reaction (qPCR). The corresponding number of mitochondrial units was calculated using the following formula according to the previous study [19 (link)]: mtDNAunits=×gramμlDNAPCR-frag(bp)×660×6.022×1023
Publication 2023
BLOOD Body Fluids Cells DNA, Mitochondrial isolation Mitochondria Plasma Real-Time Polymerase Chain Reaction
Volume management recommendations for the 20 selected patients were based on the clinical, laboratory and radiological findings, taking into consideration the IVC US data (Figure 1). Patients with very small IVCmax values (≤0.7 cm) may have small changes in IVC diameters with respiration and still may be hypovolemic [11 (link)]. If the IVC CI was ≥50% or the IVCmax was ≤0.7 cm, suggesting hypovolemia [10 (link),11 (link)], additional volume administration was recommended as clinically indicated and documented as received. If the IVC CI was <20% and the IVCmax was >0.7 cm, suggesting intravascular volume overload [10 (link),11 (link)], volume as saline, albumin and/or blood products was restricted, and diuretics, therapeutic paracentesis, and/or ultrafiltration were recommended and received as clinically indicated. Those with IVC CI ≥20–<50% and IVCmax >0.7 cm were assumed to be neither overtly hypovolemic nor hypervolemic and had ongoing volume losses estimated and replaced taking into account the isonatremic equivalents of body fluid losses and parenteral and enteral inputs [12 ], with clinical, laboratory and radiological findings taken into consideration.
Available serum creatinine levels from the earliest recorded value until the final follow-up time were obtained from medical records. The change in serum creatinine from peak values within 3 days before or after the IVC US until 4–5 days after the IVC US, in the absence of hemodialysis therapy, was used to assess response to post-IVC US volume management. A ≥ 20% decrease in serum creatinine from the peak value within 48–72 h of the therapeutic intervention [9 (link)], not resulting from hemodialysis therapy, was defined as hypovolemic- or hypervolemic-AKI [1 ]. An increasing serum creatinine or a decrease <20% from the peak value, or requirement for dialysis therapy, was defined as AKI not responsive to volume management [1 ,9 (link)].
Publication 2023
Albumins BLOOD Body Fluids Clinical Laboratory Services Creatinine Dialysis Diuretics Hemodialysis Hypovolemic Intestines, Small Paracentesis Parenteral Nutrition Patients Respiration Saline Solution Serum Therapeutics Ultrafiltration X-Rays, Diagnostic

Top products related to «Body Fluids»

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The AxyPrep Body Fluid Viral DNA/RNA Miniprep Kit is a laboratory equipment product designed for the rapid and efficient extraction of viral DNA or RNA from various body fluid samples. The kit utilizes a silica-based membrane technology to purify the target nucleic acids, providing a simple and reliable method for sample preparation.
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The QIAamp DNA Mini Kit is a laboratory equipment product designed for the purification of genomic DNA from a variety of sample types. It utilizes a silica-membrane-based technology to efficiently capture and purify DNA, which can then be used for various downstream applications.
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The QIAamp DNA Blood Mini Kit is a laboratory equipment designed for the extraction and purification of genomic DNA from small volumes of whole blood, buffy coat, plasma, or serum samples. It utilizes a silica-based membrane technology to efficiently capture and wash DNA, while removing contaminants and inhibitors.
Sourced in China, United States
The Body Fluid Viral DNA/RNA Miniprep Kit is a product designed to isolate and purify viral DNA or RNA from various body fluid samples, such as serum, plasma, or saliva. The kit utilizes a simple and efficient process to extract the target genetic material, making it suitable for applications that require high-quality viral nucleic acid samples.
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NaCl is a chemical compound commonly known as sodium chloride. It is a white, crystalline solid that is widely used in various industries, including pharmaceutical and laboratory settings. NaCl's core function is to serve as a basic, inorganic salt that can be used for a variety of applications in the lab environment.
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The S-4800 is a high-resolution scanning electron microscope (SEM) manufactured by Hitachi. It provides a range of imaging and analytical capabilities for various applications. The S-4800 utilizes a field emission electron gun to generate high-quality, high-resolution images of samples.
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Hydrochloric acid is a commonly used laboratory reagent. It is a clear, colorless, and highly corrosive liquid with a pungent odor. Hydrochloric acid is an aqueous solution of hydrogen chloride gas.
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NaHCO3 is a chemical compound that is commonly used as a laboratory reagent. It is a white, crystalline powder with the chemical formula NaHCO3. NaHCO3 is a salt that is composed of sodium (Na+) and bicarbonate (HCO3-) ions.
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The IRAffinity-1 is a Fourier transform infrared (FTIR) spectrometer designed for high-performance infrared analysis. It features a compact and robust design, provides a wide spectral range, and delivers precise and reliable measurements.

More about "Body Fluids"

Body fluids are the various aqueous solutions and secretions found within the human body, including blood, urine, cerebrospinal fluid, synovial fluid, and others.
These biological liquids serve critical physiological functions, such as transporting nutrients, facilitating waste removal, and enabling proper organ and tissue function.
Analyzing the composition and characteristics of corporeal fluids can provide valuable insights into an individual's health status and assist in the diagnosis and monitoring of various medical conditions.
Researchers often study body fluids to better understand the underlying mechanisms of disease, develop new diagnostic tools, and explore potential therapeutic interventions.
This area of study is crucial for advancing medical knowledge and improving patient outcomes.
Synonyms for body fluids include corporeal liquids, physiological solutions, and bodily secretions.
Related terms include biofluids, biospecimens, and biomarkers.
Abbreviations commonly used in this field include BF, CF (cerebrospinal fluid), and SF (synovial fluid).
Key subtopics include fluid composition analysis, disease biomarker identification, and sample collection/preparation techniques.
The AxyPrep Body Fluid Viral DNA/RNA Miniprep Kit, QIAamp DNA Mini Kit, and QIAamp DNA Blood Mini Kit are some examples of products used for extracting and purifying nucleic acids from body fluid samples.
Additionally, sodium chloride (NaCl), hydrochloric acid (HCl), and sodium bicarbonate (NaHCO3) are commonly used reagents in body fluid research and analysis.
Leveraging the power of data-driven insights, tools like PubCompare.ai can help optimize your body fluids research by identifying the most effective protocols from literature, preprints, and patents.
This AI-driven platform enables researchers to make informed decisions, enhancing the accuracy and efficiency of their studies.
Remember, a typo can happen even to the best of us: 'Reseachers' often study body fluids to better understand the underlying mechanisms of disease.