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Hyalin Substance

Hyalin substance, a specialized type of extracellular matrix, plays a crucial role in the developlment and function of various tissues and organs.
This substance is composed of glycoproteins and provides structural support, cell adhesion, and signaling cues essential for proper tissue homeostasis.
Hyalin substance research aims to elucidate its composition, biosynthesis, and interactions with other cellular components, ultimately enhancing our understanding of normal and pathological processes involving this important biological material.
Researchers can leverage AI-powered tools like PubCompare.ai to efficiently locate and analyze the best reproducible protocols for hyalin substance optimization from literature, preprints, and patents, thereby improving the accuracy and efficiency of their investigations.

Most cited protocols related to «Hyalin Substance»

1
Histological Grading of Meniscus Degeneration

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Publication 2011
Calcium Cells Collagen Cyst Donors Fast Green Femur Fibrosis Hyalin Substance Hyperplasia hypoplasia Meniscus Physiologic Calcification safranine T Tears Tibia Tissues Vision
2
Pulmonary Pathology Scoring of SARS-CoV-2 Infection
Animals were anesthetized and perfused transcardially with PBS, followed by zinc formalin. Lungs were fixed in zinc formalin. For routine histology, tissue sections (~4 μm each) were stained with hematoxylin and eosin. The following criteria were used for scoring edema, hyaline membrane formation and necrotic cellular debris: 0- none; 1-uncommon detection in <5% lung fields (200x); 2- detectable in up to 33% of lung fields; 3- detectable in up to 33-66% of lung fields; 4- detectable in >66% of lung fields. For scoring neutrophil infiltration: 0- within normal limits; 1-scattered PMNs sequestered in septa; 2- #1 plus solitary PMNs extravasated in airspaces; 3-#2 plus small aggregates in vessel and airspaces. For scoring mononuclear infiltrates, thrombosis and hemorrhage: 0-none; 1- uncommon detection in <5% lung fields (200x); 2- detectable in up to 33% of lung fields; 3- detectable in up to 33-66% of lung fields; 4- detectable in >66% of lung fields.
For SARS-CoV-2 antigen detection, slides were incubated with blocking reagent (10% normal goat serum x 30 minutes) followed by rabbit monoclonal antibody against SARS-CoV2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako) and diaminobenzidine (Dako) as chromogen. Tissues were examined and scored in a post-examination method of masking by a boarded experimental pathologist32 . Ordinal scores for lesion parameters were assigned using the following tiers: 0 = within expected limits; 1 - uncommon, <5%; 2 - detectable in 5-33%; 3 - detectable in 34-66% and 4 - detectable in >66% of lung fields (200x objective magnification).
Zheng J., Wong L.Y., Li K., Verma A.K., Ortiz M., Wohlford-Lenane C., Leidinger M.R., Knudson C.M., Meyerholz D.K., McCray PB J.r, & Perlman S. (2020). COVID-19 Treatments and Pathogenesis Including Anosmia in K18-hACE2 mice. Nature, 589(7843), 603-607.
Publication 2020
1-(1-pyrrolidinylmethyl)-2-naphthol Animals Antigens azo rubin S Biopharmaceuticals Blood Vessel Cells Edema Eosin Formalin Goat Hemorrhage Hyalin Substance isononanoyl oxybenzene sulfonate Lung Monoclonal Antibodies Necrosis Neutrophil Infiltration nucleoprotein, Measles virus Rabbits SARS-CoV-2 Serum Severe Acute Respiratory Syndrome Technique, Dilution Thrombosis Tissue, Membrane Tissues Zinc
3
Histological Analysis of ACI Cartilage Repair

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Publication 2009
Adult Antibodies Antigens Arthroscopy Autopsy Avidin Biopsy Biotin Bone Marrow Bones Cartilage Cartilages, Articular Cells Cell Shape Clone Cells Cloning Vectors Collagen Collagen Type I Collagen Type II Condyle Eosin Femur Femur Heads Fibrocartilage Fibrosis Formalin Freezing Frozen Sections Glycosaminoglycans Grafts Homozygote Hyaline Cartilage Hyalin Substance Hyaluronidase Hybridomas Immunoglobulins Immunohistochemistry Joints Joints, Ankle Knee Light Methanol Mus Needles Nitrogen Operative Surgical Procedures Oryctolagus cuniculus Paraffin Paraffin Embedding Patella Pathologic Neovascularization Patients Peroxidase Peroxides Phosphates Physiologic Calcification Procollagen PRSS2 protein, human Rabbits Saline Solution Serum Sodium Acetate Sodium Chloride Tissues Tolonium Chloride Tritium Trypsin Wound Healing
4
Histopathological Evaluation of Acute Lung Injury
Whole lungs were harvested on day 5 following RSV challenge and fixed in 10% neutral buffered formalin (Fisher Scientific). Lungs were processed as previously described [68 (link)] and stained with H&E for routine evaluation. Representative images of lung sections were taken at 20X, 200X, and 400X magnification for each immunization regimen. Tissues were examined and scored in a manner masked to experiment groups [69 (link)]. Each sample was assessed for evidence of DAD. Histopathologically, early stages of DAD include alveolar septal injury, such as cellular sloughing, necrosis, hyaline membrane formation, hemorrhage, and early cellular infiltrates. DAD scores were assigned as follows: 1—absence of cellular sloughing and necrosis; 2—Uncommon solitary cell sloughing and necrosis; 3—Multifocal cellular sloughing and necrosis with uncommon septal wall hyalinization; 4—Multifocal cellular sloughing and necrosis with common and prominent hyaline membranes.
Schmidt M.E., Knudson C.J., Hartwig S.M., Pewe L.L., Meyerholz D.K., Langlois R.A., Harty J.T, & Varga S.M. (2018). Memory CD8 T cells mediate severe immunopathology following respiratory syncytial virus infection. PLoS Pathogens, 14(1), e1006810.
Publication 2018
Cells Formalin Hemorrhage Hyalin Substance Injuries Lung Necrosis Tissue, Membrane Tissues Treatment Protocols Vaccination
5
Histopathological Assessment of Lung Injury
The left lower lung from each mouse was fixed in 10% formalin, embedded in paraffin, cut into 5 µm sections, stained with H&E. Lung injury score was measured by a blinded pathologist with a 0 to 4 point scale according to combined assessments of inflammatory cell infiltration in the airspace or vessel wall, alveolar congestion, hemorrhage, alveolar wall thickness and hyaline membrane formation. A score of 0 represented no damage; l represented mild damage; 2 represented moderate damage; 3 represented severe damage and 4 represented very severe histological changes [24] (link).
Zhu T., Wang D.X., Zhang W., Liao X.Q., Guan X., Bo H., Sun J.Y., Huang N.W., He J., Zhang Y.K., Tong J, & Li C.Y. (2013). Andrographolide Protects against LPS-Induced Acute Lung Injury by Inactivation of NF-κB. PLoS ONE, 8(2), e56407.
Publication 2013
Blood Vessel Cells Formalin Hemorrhage Hyalin Substance Inflammation Lung Lung Injury Mus Paraffin Embedding Pathologists Tissue, Membrane

Most recents protocols related to «Hyalin Substance»

1
Histopathological Lung Evaluation in SARS-CoV-2 Hamster Model

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Publication 2023
Alveolar Epithelial Cells Animals Bronchi Bronchiolitis Bronchitis Cells Character COVID 19 Edema Eosin Epithelial Cells Ethanol Fingers Formalin Hamsters Hemorrhage Hyalin Substance Hyperplasia Lung Lymphocyte Lymphoid Tissue Macrophage Microscopy Necrosis Neutrophil Paraffin Embedding Pathologists Pneumonia Pneumonia, Interstitial Tissue, Membrane Tissues Type-II Pneumocytes Xylene
2
Histological Assessment of Murine Lung Pathology
Lungs were dissected from mice euthanized by intraperitoneal sodium pentobarbital injection as described above. The left lung lobe of each animal was removed at necropsy, instilled intratracheally with 4% paraformaldehyde (PFA), and post-fixed for 24 h by immersion in 4% PFA, prior to transfer into 70% ethanol and storage at 4 °C. Lung samples were then routinely processed to paraffin blocks and 5-µm thick serial sections prepared by cutting through the whole paraffin block and mounting sections 1:10 onto glass slides. These slides were stained with hematoxylin and eosin according to standard procedure and examined microscopically by an experienced veterinary pathologist. Microscopic lung findings were scored semi-quantitatively following accepted principles74 (link). Briefly, all serial sections per animal were first evaluated blinded to treatment at low magnification (×40 to ×100) to select the lung level(s) with the most severe and extensive lesions for each animal. The extent of the lesions across this section was then estimated (and scored as 0 = < 5%; 1 = 5–33%; 2 = 33–66%; 3 = >66%) and this parameter taken as a weighing factor to multiply with the sum of all the individually scored lesions, calculating an overall combined lung pathology score per animal. The individual lesions were scored at high magnification (×200–400) and these included alveolar interstitial inflammatory cells (neutrophils, macrophages, and/or lymphocytes/plasma cells), perivascular mixed inflammatory cell infiltrate and edema, necrosis, intra-alveolar neutrophils, macrophages, and/or hemorrhage (each scored as 0 = none; 1 = mild, 2 = moderate, 3 = severe). Alveolar septal thickening (scored as 0 = none, 1 = 2-fold increase, 2 = 2–4-fold increase, 3 = more than 4-fold increase compared with unaffected septa), hyaline membranes, and intra-alveolar proteinaceous fluid (the latter two scored as 0 = none, 1 = 1, 2 = more than 1 per alveolus) were scored as well.
Myeni S.K., Bredenbeek P.J., Knaap R.C., Dalebout T.J., Morales S.T., Sidorov I.A., Linger M.E., Oreshkova N., van Zanen-Gerhardt S., Zander S.A., Enjuanes L., Sola I., Snijder E.J, & Kikkert M. (2023). Engineering potent live attenuated coronavirus vaccines by targeted inactivation of the immune evasive viral deubiquitinase. Nature Communications, 14, 1141.
Publication 2023
Alveolar Epithelial Cells Animals Autopsy Edema Eosin Ethanol factor A Hemorrhage Hyalin Substance Inflammation Injections, Intraperitoneal Lobar Pneumonia Lung Lymphocyte Macrophage Microscopy Mus Necrosis Neutrophil Paraffin paraform Pathologists Pentobarbital Sodium Pericytes Plasma Cells Proteins Submersion Tissue, Membrane Tooth Socket
3
Histopathological Analysis of Diffuse Alveolar Damage
Two experienced pathologists (M.O. and M.K.) reviewed the archived haematoxylin and eosin (HE)-stained slides to confirm the findings of DAD. One representative paraffin block containing the alveolar region, reflecting the acute phase of DAD (Fig. 1a) was selected for immunohistochemical analysis in each case. Most cases showed acute phase DAD lesions as well as organizing phase DAD lesions (Fig. 1b). In some cases, lesions only showing the acute phase of DAD changes were found, with no appreciable lesions showing the organizing phase of DAD changes. At the same time, areas that were thought to be more affected by smoking, such as emphysematous areas, were excluded. All the tissue samples were fixed in formalin and embedded in paraffin. Serial tissue Sects. (3 μm-thick) were prepared for HE staining and immunohistochemical analysis.

Histopathology of autopsy specimens of the acute phase (a) and the organizing phase (b) of diffuse alveolar damage (DAD). Haematoxylin and eosin staining. In the acute phase, oedema and hyaline membrane formation are observed (a). In the organizing phase, granulation tissue forms in the alveolar walls and spaces (b). Original magnification × 100

Ohya M., Tateishi A., Matsumoto Y., Satomi H, & Kobayashi M. (2023). Bystander CD8 + T cells may be involved in the acute phase of diffuse alveolar damage. Virchows Archiv, 482(3), 605-613.
Publication 2023
Autopsy Edema Eosin Formalin Granulation Tissue GZMB protein, human Hematoxylin Hyalin Substance Paraffin Paraffin Embedding Pathologists Pulmonary Emphysema Tissue, Membrane Tissues
4
Histopathological Evaluation of Lung Lesions
Tissue samples were fixed in formalin, processed routinely, and stained with
hematoxylin and eosin. Histologic scores and diagnoses were made by consensus
(LAJH or LS, and JLC) without knowledge of the gross diagnosis or microbiology
data. All lung sections were scored for the presence or absence of specified
histologic lesions (Supplemental Tables S4 and S5). The duration of cranioventral
and caudodorsal lung lesions was classified as acute (absence of fibrosis),
subacute (presence of immature granulation tissue), or chronic (presence of
mature fibrosis that was densely eosinophilic with fewer and smaller fibroblast
nuclei).
Histologic criteria for diagnosis of bronchopneumonia were neutrophils and
macrophages filling the lumen of alveoli and bronchioles. Histologic criteria
for alveolar and bronchiolar damage (a form of interstitial/bronchointerstitial
lung disease) were alveoli lined by hyaline membranes or type II pneumocytes and
loss of bronchiolar epithelium with attenuation of remaining epithelial cells,
respectively. Cases were diagnosed as BIP if bronchopneumonia was a predominant
histologic lesion in sections of cranioventral lung and alveolar and bronchiolar
damage were prominent in sections of caudodorsal lung. However, the lesion types
were not required to be anatomically segregated: The cranioventral lung sections
of some BIP cases had alveolar and bronchiolar damage in addition to the
bronchopneumonia required for the diagnosis of BIP, and the caudodorsal lung
sections of some BIP cases had bronchopneumonia in addition to the alveolar and
bronchiolar damage required for the diagnosis of BIP.
Haydock L.A., Fenton R.K., Sergejewich L., Veldhuizen R.A., Smerek D., Ojkic D, & Caswell J.L. (2023). Bronchopneumonia with interstitial pneumonia in beef feedlot cattle: Characterization and laboratory investigation. Veterinary Pathology, 60(2), 214-225.
Publication 2023
Bronchioles Bronchopneumonia Diagnosis Eosin Eosinophilia Epithelial Cells Epithelium Fibrosis Formalin Granulation Tissue Hyalin Substance Lung Neutrophil Tissue, Membrane Tissues Tooth Socket Type-II Pneumocytes
5
Renal Biopsy Analysis via ISN/RPS Classification
The histological characteristics of each renal biopsy were interpreted according to the ISN/RPS classification [28 (link)]. We divided patients into two groups according to their interstitial inflammation scores (II score)—0, 1 as a low II score; and 2, 3 as a high II score. Their activity and chronicity were compared.
The pathologist (Shu YJ) also checked cropped 2D nuclear/membrane images every 10μm by the ISN/RPS Classification. The checked items contained all components of chronicity and parts of activity, including II, endocapillary hyperplasia, and cellular/fibrocellular crescent. The fibrinoid necrosis, hyaline deposit, and neutrophils/karyorrhexis cannot be interpreted clearly in the cropped 2D nuclear/membrane images.
Huang Y.C., Hsu Y.C., Chen J.P, & Fu L.S. (2023). The Role of New 3D Pathology and Lymphocyte Expression of Interstitial Inflammation in Pediatric-Onset Lupus Nephritis. International Journal of Molecular Sciences, 24(4), 3512.
Publication 2023
Cells Hyalin Substance Hyperplasia Inflammation Kidney Necrosis Neutrophil Nuclear Envelope Pathologists Patients

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1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Hematoxylin and eosin by Merck Group
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Hematoxylin and eosin (H&E) is a commonly used staining method in histology and pathology. Hematoxylin stains cell nuclei blue, while eosin stains the cytoplasm and extracellular matrix in various shades of pink and red. This staining technique is used to provide contrast and enable the visualization of cellular and tissue structures under a microscope.
3
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Hct116 cell line by Shanghai Institute of Biochemistry and Cell Biology
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He staining kit by Solarbio
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The HE staining kit is a laboratory reagent used to stain biological samples for microscopic examination. It consists of two solutions, Hematoxylin and Eosin, which differentially stain cell nuclei and cytoplasm, respectively, allowing for the visual identification of cellular structures.
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Optical microscope by Olympus
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More about "Hyalin Substance"

Hyalin substance, also known as hyaline substance or hyaline matrix, is a specialized type of extracellular matrix that plays a crucial role in the development and function of various tissues and organs.
This biological material is composed of glycoproteins and provides structural support, cell adhesion, and signaling cues essential for proper tissue homeostasis.
Hyalin substance research aims to elucidate its composition, biosynthesis, and interactions with other cellular components, ultimately enhancing our understanding of normal and pathological processes involving this important biological substance.
Researchers can leverage AI-powered tools like PubCompare.ai to efficiently locate and analyze the best reproducible protocols for hyalin substance optimization from literature, preprints, and patents, thereby improving the accuracy and efficiency of their investigations.
The hyalin substance is closely related to other extracellular matrix components, such as those found in the basal lamina (BL), which is a specialized type of extracellular matrix that forms a thin, sheet-like structure underlying epithelial cells and endothelial cells.
The BL is composed of laminin, collagen IV, proteoglycans, and other glycoproteins, and it provides structural support, cell adhesion, and signaling cues essential for tissue organization and function.
Hematoxylin and eosin (H&E) staining is a common histological technique used to visualize the hyalin substance and other extracellular matrix components in tissue sections.
The hematoxylin dye stains nuclei blue, while the eosin dye stains cytoplasmic and extracellular structures, including the hyalin substance, in various shades of pink and red.
Cell lines such as HCT116, SW620, and SW480, which are commonly used in cancer research, often exhibit alterations in the composition and organization of the extracellular matrix, including the hyalin substance.
Understanding the role of the hyalin substance in these cell lines can provide insights into the pathogenesis and progression of various diseases, such as cancer.
Additionally, the HEK 293T cell line, which is a commonly used human embryonic kidney cell line, has been employed in the study of the hyalin substance and its interactions with other cellular components.
The optical microscope is a valuable tool for visualizing and analyzing the hyalin substance and other extracellular matrix structures in cell and tissue samples.