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> Anatomy > Body Substance > Pancreatic Juice

Pancreatic Juice

Pancreatic juice is a complex fluid produced by the pancreas that plays a crucial role in the digestive process.
It contains a variety of enzymes, including amylase, lipase, and proteases, which help break down carbohydrates, fats, and proteins, respectively.
Pancreatic juice is secreted into the small intestine, where it mixes with food and other digestive fluids to facilitate nutrient absorption.
Understandng the composition and function of pancreatic juice is essential for researching pancreatic disorders, such as pancreatits and pancreatic cancer.
Studing pancreatic juice can also provide insights into overall gastrointestinal health and digestion.

Most cited protocols related to «Pancreatic Juice»

1
Twin-SHIME: Simulating Gut Microbiome
The SHIME is a dynamic in vitro model of the human intestinal tract, composed of five double‐jacketed vessels, respectively simulating the stomach, small intestine and the three colon regions. In this experiment, only the first colon compartment was used (Fig. 5). Two SHIME units were used in parallel ('Twin‐SHIME') in order to obtain identical environmental conditions and identical microbial composition and activities for both units (Van den Abbeele et al., 2010 (link)). Whereas the first unit consisted of the conventional set‐up that only harbours luminal microbes (= luminal SHIME or L‐SHIME), the second unit was modified by incorporating a mucosal environment (= mucosal SHIME or M‐SHIME). In order to achieve a representative mucosal surface in the M‐SHIME, 100 mucin‐covered microcosms were added per 500 ml luminal suspension. The microcosms (length = 7 mm, diameter = 9 mm, total surface area = 800 m2/m3, AnoxKaldnes K1 carrier, AnoxKaldnes AB, Lund, Sweden) were coated by submerging them in mucin agar. To simulate the renewal of the mucus layer, half of the mucin‐covered microcosms were replaced daily by sterile ones.
The ascending compartment (500 ml) from both SHIME units was inoculated with 40 ml of a 1:5 dilution of fresh stools provided by a healthy human volunteer (25 years) who had no history of antibiotic treatment 6 months before the study. Inoculum preparation was done as previously described by Possemiers and colleagues (2004 (link)). Three times per day, 140 ml SHIME feed and 60 ml pancreatic juice were added to the stomach and small intestine respectively.
Van den Abbeele P., Roos S., Eeckhaut V., MacKenzie D.A., Derde M., Verstraete W., Marzorati M., Possemiers S., Vanhoecke B., Van Immerseel F, & Van de Wiele T. (2011). Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. Microbial biotechnology, 5(1), 106-115.
Publication 2011
Agar Antibiotics Blood Vessel Colon Feces Healthy Volunteers Homo sapiens Intestines Intestines, Small Mucins Mucous Membrane Mucus Pancreatic Juice Sterility, Reproductive Stomach Technique, Dilution Twins
2
Emulsifier Impact on Gut Microbiome: M-SHIME Dynamics
The direct effect of emulsifier on intestinal microbiota was studied in an M-SHIME system, a dynamic in vitro model which simulates the lumen- and mucus-associated human intestinal microbial ecosystem (ProDigest-Ghent University, Ghent, Belgium) [13 (link), 14 (link), 15 ]. This model consists of consecutive pH controlled, stirred (200 rpm), airtight, double-jacketed glass vessels kept at 37°C and under anaerobic conditions by daily flushing with N2 (15 min). The set-up used in this study consisted of a stomach and a small intestine vessel and three proximal colon vessels in parallel. The system was operated and simulation media were prepared as described earlier [13 (link), 14 (link), 15 ]. The colon vessels were inoculated at the start with 40 mL human fecal suspension in 500 mL sterile nutritional medium (per L: 3 g yeast extract, 1 g special peptone (Oxoid, Aalst, Belgium), 4 g commercial porcin gastric mucin (Sigma-Aldrich, Bornem, Belgium) and 0.5 g L-cystein). After an initial static incubation of 18 h, 140 mL sterile nutritional medium at pH 2 per colon vessel was supplemented to the stomach vessel: this procedure was conducted three times a day. Stomach digest suspension was pumped into the small intestine vessel together with 60 mL bile and pancreatic juice [per L: 12.5 g NaHCO3, 6 g dehydrated bile extract (Oxgall, Difco) and 0.9 g pancreatin (Sigma)] per colon vessel. Small intestinal digest suspension was distributed over the different proximal colon vessels, which contained simulated colon microbiota. To simulate colon conditions, the residence time in the colon vessels was 20 h and pH was controlled at pH 6.15–6.40 which are both in the range of in vivo observations [17 (link), 18 (link)]. Mucus microbiota were simulated by adding carriers coated with agar containing commercially available porcine gastric mucin (Sigma-Aldrich) [13 (link)]. Sixty mucin agar-covered microcosms in a polyethylene netting were added to each colon vessel to obtain an appropriate mucus surface area. Every two days, two-third of the mucin agar-covered microcosms were replaced by fresh sterile ones under a flow of N2 to prevent disruption of anaerobic conditions. Seven days after inoculation, colon vessels were treated with either CMC or P80 at a final concentration of 1.00%, 0.50%, 0.25% or 0.10%. Lumen and mucin agar samples were taken every two days. Mucin agar-covered microcosms were washed with sterile PBS to remove lumen bacteria. Mucin agar was removed from microcosms, homogenized and stored immediately at −20°C until further analysis.
Chassaing B., De Bodt J., Marzorati M., Van de Wiele T, & Gewirtz A.T. (2017). Dietary emulsifiers directly alter human microbiota composition and gene expression ex vivo potentiating intestinal inflammation. Gut, 66(8), 1414-1427.
Publication 2017
Administration, Mucosal Agar Bacteria Bicarbonate, Sodium Bile Blood Vessel Colon Colonic Diseases Ecosystem Feces Homo sapiens Intestinal Microbiome Intestinal Mucosa Intestines Intestines, Small Microbial Community Mucins Mucins, Gastric Mucus Pancreatic Juice Pancreatin Peptones Pigs Polyethylene Sterility, Reproductive Stomach Vaccination Yeast, Dried
3
Defined Medium for Gut Microbiome Simulation
The defined medium (DM) was purchased from ProDigest (Ghent, Belgium). It contained (in gL-1) arabinogalactan (1.2), pectin (2.0), xylan (0.5), resistant starch (4.0), glucose (0.4), yeast extract (3.0), peptone (1.0), mucin (3.0), and cysteine (0.5). The defined medium was adjusted to pH 2 prior to autoclaving. Pancreatic juice contained (in gL-1) NaHCO3 (12.5) (Sigma-Aldrich, Saint Louis, MO), bile salts (6.0) (BD, Franklin Lakes, NJ), and pancreatin (0.9) (Sigma-Aldrich, Saint Louis, MO). Mucin-Agar containing carriers were prepared by dipping plastic, hollowed carriers (DI, 5 mm; ProDigest) into a mucin-agar solution. The Mucin-Agar solution was prepared by boiling 1% bacterial agar in autoclaved MilliQ water three times, to dissolve 5% type II porcine mucin (Sigma-Aldrich). The filled carriers were allowed to solidify under laminar flow in a biosafety cabinet at room temperature and stored at -4 °C until use.
Fecal samples (Microbiome Health Research Institute; Boston, MA) were harvested from an individual American, randomly selected from a pool of typical, Western diet consumers, between the ages of 21 and 45 years old, with an average Body Mass Index (BMI), who had been antibiotics-free for at least 1 year. According to the provider, the donor of the fecal sample was required to fill out a medical history questionnaire and interviewed in a process similar to that used for blood donors. A series of tests were performed on both fecal and blood samples to screen for any potentially infectious pathogens before deposition of fecal material. Sixty days post donation, a second round of tests were performed on both the fecal and blood samples. Upon both sets of tests proving negative for the presence of pathogens, the fecal sample thus collected was homogenized to make a 10% feces in glycerol buffer solution. The homogenate was then aliquoted into 250 mL containers, frozen, and stored at -80°C (http://www.Openbiome.org, cited December 01, 2016). The same homogenate was used to inoculate both systems, simultaneously, for each experiment.
Liu L., Firrman J., Tanes C., Bittinger K., Thomas-Gahring A., Wu G.D., Van den Abbeele P, & Tomasula P.M. (2018). Establishing a mucosal gut microbial community in vitro using an artificial simulator. PLoS ONE, 13(7), e0197692.
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Publication 2018
Agar Antibiotics Bacteria Bicarbonate, Sodium BLOOD Buffers Cysteine Donor, Blood Feces Freezing galactoarabinan Glucose Glycerin Index, Body Mass Infection Methoxypectin Microbiome Mucin-3 Mucins Pancreatic Juice Pancreatin pathogenesis Peptones Pigs Resistant Starch Salts, Bile Tissue Donors Xylans Yeast, Dried
4
Pancreatic Fluid Protein Extraction
In brief, pancreatic fluid samples were collected on ice, centrifuged at 4°C at 14,000 rpm to remove cellular debris, and aliquoted (500 μL) prior to storage at -80°C. Protein concentration was determined using the BioRAD protein assay according to the manufacturer's instructions. In preparation for SDS-PAGE analysis, the proteins from pancreatic fluid specimens were isolated by precipitation with the addition of 12.5% trichloroacetic acid (TCA). This process limits protein degradation by instantaneously deactivating enzymes and removing salts that will interfere with the subsequent molecular weight-based fractionation by SDS-PAGE, as described below. Pellets were re-dissolved in 50 μL of reducing Laemmli buffer (with 10 mM dithiothreitol) and alkylated with 1% acrylamide for subsequent GeLC-MS/MS.
Paulo J.A., Lee L.S., Wu B., Repas K., Mortele K.J., Banks P.A., Steen H, & Conwell D.L. (2010). Identification of Pancreas-Specific Proteins in Endoscopic (ePFT) Collected Pancreatic Fluid with Mass Spectrometry (GeLC-MS/MS). Pancreas, 39(6), 889-896.
Publication 2010
Acrylamide Biological Assay Cells Dithiothreitol Enzymes Fractionation, Chemical Laemmli buffer Pancreatic Juice Pellets, Drug Proteins Proteolysis Salts SDS-PAGE Tandem Mass Spectrometry Trichloroacetic Acid
5
Improving Diabetes Risk Prediction with EHR Data

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Publication 2015
A-factor (Streptomyces) Acanthosis Nigricans Angina Pectoris Atherosclerosis Candidiasis, Cutaneous Cataract Childbirth Complications of Diabetes Mellitus Diabetes Mellitus Diabetic Polyneuropathies Diabetic Retinopathy Diagnosis factor A Foot Ulcer Generic Drugs Genitalia, Female Gingiva Glucophage Heart Heart Diseases High Blood Pressures Hyperlipidemia Hypersensitivity Lipid Metabolism Disorders Menstruation Disturbances Metformin Myocardial Infarction Myocardial Ischemia Nails Obesity Pancreatic Cancer Pancreatic Juice Parenteral Nutrition, Total Patients Periodontal Diseases Pharmaceutical Preparations Phenotype Physicians Polycystic Ovary Syndrome Population Group Pregnancy in Diabetics Skin Diseases, Infectious Subcutaneous Fat Trigeminal Nerve Disorder

Most recents protocols related to «Pancreatic Juice»

1
Comprehensive MRI Examination Protocol for Pancreatic Lesions
All of the MRI examinations were performed using 3T MRI systems (Magnetom Vida, Siemens Healthineers, Erlangen, Germany) with a 30-channel surface coil and 32- or 72-channel spine coil. Breath-hold half-Fourier acquisition single-shot turbo spin echo (HASTE) heavily T2-weighted imaging was obtained to evaluate the structures containing fluid such as the pancreatic duct and cyst, respiratory-triggered fast spin echo T2WI with fat suppression was obtained to evaluate the overall anatomical structure, and 3D gradient echo volumetric interpolated breath-hold examination (VIBE) T1-weighted imaging (T1WI) based on in- and opposed-phase was obtained to detect the fat component in the lesion, while diffusion-weighted imaging (DWI) with b values of 0, 50, 400, 800, and 1000 s/mm2 was obtained to acquire information about the cellularity or nature of tissue inside the lesion. For the DCE-MRI, 0.1 mmol/kg of gadoterate meglumine (Dotarem, Guerbet, Paris, France) was injected at a rate of 1.5 mL/s using a power injector, followed by a 20 mL saline flush. DCE-MRI was acquired using free-breathing golden-angle radial sparse parallel (GRASP) imaging. The temporal resolution was 13.5 s for the first 24 s, and then 8.4 s for 180 s and 13.5 s for the last 121 s. After performing the DCE-MRI, we also performed delayed-phase coronal and sagittal contrast-enhanced T1WI with a higher spatial resolution than DCE_MRI to obtain additional information about the lesion or anatomical structure. The detailed MR parameters are summarized in Table 1 and most of the sequences were performed with the commonly used MRI technique.
Choi M., Yoon S., Lee Y, & Han D. (2023). Evaluation of Perfusion Change According to Pancreatic Cancer and Pancreatic Duct Dilatation Using Free-Breathing Golden-Angle Radial Sparse Parallel (GRASP) Magnetic Resonance Imaging. Diagnostics, 13(4), 731.
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Publication 2023
Cells Cyst Diffusion Dotarem ECHO protocol Flushing gadoterate meglumine Pancreatic Juice Physical Examination Respiratory Rate Saline Solution Tissues Vertebral Column
2
Probiotic Potential of β-Galactosidase Isolates
In vitro studies were undertaken to evaluate the probiotic potentiality of β-galactosidase-producing isolates as per FAO/WHO (8 ). All eight isolates were subjected to simulated gastric juice and bile tolerance tests. The simulated gastric juice contained 0.3% w/v pepsin and 0.5% w/v NaCl (Himedia Laboratories Pvt, Ltd.), at pH 2 or 4. A total of 1 ml of cell suspension was centrifuged (12,000 x g, 5 min at 5˚C), inoculated into 10 ml of gastric juice with pH 2 or 4, incubated at 37˚C for 3 h and cell viability was measured as colony-forming units (CFU) by plating technique, and the percentage of survival was calculated as follows:
Bile tolerance was determined by inoculating each strain (1% v/v) into MRS broth with 0.3% (w/v) of bile salt (Oxgall; Himedia Laboratories Pvt, Ltd.) and incubated for 3 h at 37˚C. Viability was measured as CFU by plating technique and compared with the control (without bile salt) (13 (link),21 (link)).
Pancreatic enzyme tolerance was calculated according to a study by Rashmi and Gayathri (13 (link)), with slight modifications. Overnight cultures were centrifuged at 6,000 x g (20 min at 5˚C), inoculated into simulated pancreatic juice (SPJ; bile 3 g/l and pancreatin 0.1 g/l, sodium phosphate dibasic heptahydrate 50.81 g/l, and NaCl 8.5 g/l in a KH2PO4 buffer at pH 8.0; Himedia Laboratories Pvt, Ltd.), incubated at 37˚C for 3 h and the percentage of survival was calculated as follows:
Vasudha M., Prashantkumar C.S., Bellurkar M., Kaveeshwar V, & Gayathri D. (2023). Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization. Biomedical Reports, 18(3), 23.
Publication 2023
beta-Galactosidase Bile Buffers Cells Cell Survival Enzymes Immune Tolerance Juices, Gastric Pancreas Pancreatic Juice Pancreatin Pepsin A Probiotics Salts, Bile Sodium Chloride sodium phosphate Strains
3
Acute Biliary Pancreatitis Assessment and Management
All acute biliary pancreatitis and complications in our hospital were diagnosed according to the Atlanta pancreatitis classification (as revised in 2012). The presence of gallstones with or without choledocholithiasis was confirmed by imaging examination. All patients underwent ERCP lithotomy and preventive pancreatic stent placement, and pancreatic juice was obtained for bacterial culturing and drug sensitivity testing. The included patients were older than 18 years old. We included patients with a first-time AP infection and those with AP who could be followed-up for 6 months.
Zhao C., Yao Y., Yao W., Hao Q., Chen L, & Wang Z. (2023). Distribution analysis of positive and negative pathogenic bacteria in patients with acute pancreatitis and the clinical characteristics and model prediction analysis of positive infection bacteria. Annals of Translational Medicine, 11(2), 98.
Publication 2023
Bacteria Choledocholithiasis Cholelithiasis Endoscopic Retrograde Cholangiopancreatography Hypersensitivity Infection Pancreas Pancreatic Juice Pancreatitis Pancreatitis, Acute Patients Stents
4
Endoscopic Pancreatic Duct Examination Protocol
All endoscopic operations were completed by doctors with more than 10 years of surgical experience. The surgical procedure was as follows. Firstly, the endoscope reached the descending segment of the duodenum through the gastric cavity. Next, the surgeon looked for the great duodenal papilla on the inner side of the duodenum, rinsed the duodenum with normal saline, cut the knife before the sphincterotomy of the duodenal papilla, and then intubated the pancreatic duct through the guide wire. After successful intubation, an X-ray was used to confirm that the guide wire was placed along the direction of the pancreatic duct, and followed-up the incision knife to suck pancreatic juice. During the whole process, the lumen was filled with normal saline to avoid intestinal fluid pollution.
The interrupted pancreatic juice was extracted, placed into a sterile vacuum specimen collection tube, and then stored in an incubator. All specimens were sent to the laboratory for examination within 8 hours. The BacT/Alert3D blood culture and vitek-2compact bacterial identification and drug sensitivity instruments (Biomerie, France) were utilized for bacterial culturing of the pancreatic juice. The reagents applied were specifically for the instrument.
Zhao C., Yao Y., Yao W., Hao Q., Chen L, & Wang Z. (2023). Distribution analysis of positive and negative pathogenic bacteria in patients with acute pancreatitis and the clinical characteristics and model prediction analysis of positive infection bacteria. Annals of Translational Medicine, 11(2), 98.
Publication 2023
Bacteria Blood Culture Dental Caries Duodenum Endoscopes Hypersensitivity Intestines Intubation Nipples Normal Saline Operative Surgical Procedures Pancreatic Duct Pancreatic Juice Pharmaceutical Preparations Physicians Radiography Specimen Collection Sphincterotomy Sterility, Reproductive Stomach Surgeons Surgical Endoscopy Vacuum
5
ERCP Management of Acute Pancreatitis
All patients were treated according to the Chinese guidelines for the diagnosis and treatment of AP: after admission, the patients were fasted, rehydrated, and given analgesia, enzyme inhibition, and other treatments. In addition to the above conservative treatment measures, patients with ERCP indications underwent ERCP examination and treatment within 72 hours after admission. Pancreatic stents were placed in high-risk groups to prevent post-ERCP pancreatitis, and pancreatic juice was collected for bacterial culturing.
Zhao C., Yao Y., Yao W., Hao Q., Chen L, & Wang Z. (2023). Distribution analysis of positive and negative pathogenic bacteria in patients with acute pancreatitis and the clinical characteristics and model prediction analysis of positive infection bacteria. Annals of Translational Medicine, 11(2), 98.
Publication 2023
Bacteria Chinese Conservative Treatment Diagnosis Endoscopic Retrograde Cholangiopancreatography Enzymes Management, Pain Pancreas Pancreatic Juice Pancreatitis Patients Psychological Inhibition Stents

Top products related to «Pancreatic Juice»

1
Jf 260v by Olympus
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2
Pancreatin by Merck Group
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Pancreatin is a digestive enzyme complex derived from the pancreas of mammals. It contains a mixture of digestive enzymes, including amylase, lipase, and protease, which play a role in the breakdown of carbohydrates, fats, and proteins, respectively.
3
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α amylase by Merck Group
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More about "Pancreatic Juice"

Pancreatic secretions, exocrine pancreas fluids, digestive enzymes, pancreatopathy, pancreatic ductal adenocarcinoma (PDAC), pancreatic acinar cells, pancreatic proteome, pancreatobiliary disorders, pancreatic fluid analysis, pancreatic biomarkers, pancreatic enzyme replacement therapy (PERT), pancreatic lipase, pancreatic amylase, pancreatic proteases, pancreatic duct, pancreatic insufficiency, pancreatic exocrine function, pancreatology, TJF-260V duodenoscope, QuantiFluor dsDNA/RNA Systems, MagNA Pure Compact nucleic acid extraction, GloMax Discover luminescence detection, TNF-γ cytokine, Torrent Suite Software v.5.0 for NGS, α-amylase enzyme activity.
Pancreatic juice is a complex, enzyme-rich fluid secreted by the exocrine pancreas that plays a crucial role in the digestive process.
This fluid contains a variety of digestive enzymes, including amylase for carbohydrate breakdown, lipase for fat digestion, and proteases for protein catabolism.
Pancreatic juice is released into the small intestine, where it mixes with food and other digestive fluids to facilitate nutrient absorption.
Understanding the composition and function of pancreatic juice is essential for researching pancreatic disorders, such as pancreatitis and pancreatic cancer (PDAC).
Analyzing pancreatic fluid can also provide insights into overall gastrointestinal health and digestion.
Researchers may employ specialized tools and techniques, like the TJF-260V duodenoscope, QuantiFluor dsDNA/RNA Systems, MagNA Pure Compact, GloMax Discover, and Torrent Suite Software, to study the pancreatic proteome, biomarkers, and enzyme activity.
Optimizing research protocols and identifying the optimal products and procedures can significantly advance the understanding of pancreatic juice and its role in health and disease.
PubCompare.ai's AI-powered tools can assist researchers in locating the best protocols from literature, pre-prints, and patents, enabling them to optimize their pancreatic juice studies and drive progress in pancreatology and gastroenterology.