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Hemic System

The Hemic System is a complex network of cells, tissues, and organs responsible for the production, transport, and regulation of blood and blood components.
This system plays a crucial role in the body's immune response, circulation, and homeostasis.
It includes the bone marrow, spleen, lymph nodes, and circulatory vessels that carry blood throughout the body.
The Hemic System is essential for the delivery of oxygen, nutrients, and other vital substances, as well as the removal of waste products.
Researchers studying the Hemic System often utilize a variety of analytical techniques, including AI-driven analysis, to optimize protocols, compare products, and identify the best solutions for their research needs.
This approach can help enhance the accuracy and reproducibility of Hemic System research, leading to improved understandig of this critical physiological system.

Most cited protocols related to «Hemic System»

Having undertaken a number of studies27, 28, 29, 30, 31, 32, 33, 34, 35, 36 that generated experience and data from genomic and GEX‐based classification of primary breast cancers, in 2009 the academic group in Lund and the SSBCG formed SCAN‐B, an initiative to move these analyses closer to today's patients and eventually into routine clinical practice37. The mission was that all patients with newly diagnosed breast cancer would be offered these analyses within the clinical context, and that the analysis should be performed on the most modern platforms (NGS) using fresh (not formalin‐fixed) tumour tissue samples in order to utilize the full potential of deep sequencing (Fig. 1). To be clinically meaningful, sampling and shipping of fresh tumour specimens was proposed to be continuous and in real time, and fully integrated into ordinary surgical and pathological practices, with delivery of the results from the analysis to the clinician at the time of the postoperative conference. The initial research programme for the SCAN‐B initiative included consecutive retrieval of fresh tissue, and preoperative and postoperative blood samples.
The SSBCG acknowledged the initiative in 2009. The initiative had to be considered as a research project open to all patients with breast cancer. Ethical approval was given in 2009 and all (now centralized to 7) surgical departments in the South Swedish Health Care Region agreed to put necessary resources at hand for the project. Funding of technical equipment and reagents was granted by the Mrs Berta Kamprad Foundation, which provided a stable basis for the initial years of operation.
In the same interval, six regional cancer centres (RCCs) were established in Sweden to improve cancer care and clinical development38. In 2010, the counties within RCC South, identical to the catchment area of the SSBCG, reached a decision and economic agreement that systematic biobanking of blood and unfixed tumour tissue from all consenting patients with cancer should be implemented as a support for clinical cancer research. To support this, an infrastructure for blood and tumour tissue collection and storage was established at all major hospitals in southern Sweden. This infrastructure was not limited to breast cancer research, and has also been implemented for other cancer diagnoses such as lung cancer39. The decision and support of the regional governing body were instrumental in implementation of the same routines across a large geographical area, encompassing seven major hospitals and covering a population of 1.8 million (Fig. 1).
The start of this project was thus a fruitful partnership between enthusiastic researchers, an established collaborative group, seven hospitals, an important funding body and the regional governing body.
Publication 2018
BLOOD Conferences Diagnosis Formalin Fruit Genome Hemic System Human Body Lung Malignant Neoplasm of Breast Malignant Neoplasms Neoplasms Obstetric Delivery Patients Radionuclide Imaging Tissues
Ae. albopictus mosquitoes (kindly provided by Illia Rochlin, Suffolk County Health Department, Yaphank, NY, USA) were originally collected in Suffolk County in 2014 and subsequently colonized in the NYSDOH Arbovirus Laboratory. F5–F7 female mosquitoes from New York were used for experimental feedings. Ae. aegypti mosquitoes used for preliminary experiments were collected by C. Mangudo in Salta, Argentina, in 2014 and initially colonized by V. Micieli and L.D. Kramer at the Centro de Estudios de Parasitología y Vectores (La Plata, Argentina) before being shipped to the NYSDOH Arbovirus Laboratory for maintenance. F4–F5 females from Argentina were used for experimental feedings. Ae. aegypti mosquitoes (kindly provided by G.D. Ebel, Colorado State University, Fort Collins, CO, USA) were originally collected in Poza Rica, Mexico. F7–F8 females from Mexico were used for experimental feedings. For preliminary blood feeding experiments, Ae. aegypti mosquitoes from Argentina were fed Zika virus PR stock virus diluted 1:1, 1:5, or 1:20 in defibrinated sheep blood (Colorado Serum Co., Denver, CO, USA) with 2.5% sucrose. For feedings with freshly propagated virus, supernatant from infected C6/36 cultures was harvested at 96 h after infection (multiplicity of infection ≈1.0) and diluted 1:1 with blood-sucrose mixture without freezing. Female mosquitoes, 4–7 days of age, were deprived of sucrose for 18–24 h and offered blood meal mixtures by use of a Hemotek membrane feeding system (Discovery Workshops, Acrington, UK) with a porcine sausage casing membrane. For all subsequent experiments assessing dose-dependent vector competence, similarly prepared fresh C6/36 cultures of Zika virus HND and Zika virus CAM were used to feed Ae. aegypti mosquitoes from Mexico and Ae.albopictus mosquitoes from New York. In addition to undiluted supernatant, 1:20, 1:400, and 1:8,000 dilutions were made in C6/36 maintenance media before being mixed with blood.
For all blood feeding experiments, mosquitoes were sedated with CO2 after 1 h of feeding, and fully engorged mosquitoes were transferred to 0.6-L cartons and maintained at 27°C for experimental testing. Infection, dissemination, and transmission rates were determined as previously described (24 (link)) on day 14 or 21 after feeding. After the mosquitoes were sedated, the legs were removed from 12–30 mosquitoes and placed in 1 mL mosquito diluent (20% heat-inactivated fetal bovine serum in Dulbecco phosphate-buffered saline plus 50 μg/mL penicillin/streptomycin, 50 μg/mL gentamicin, and 2 μg/mL Fungizone [Sigma Aldrich, St. Louis, MO, USA]). For 30 minutes, mosquitoes were allowed to expectorate into capillary tubes containing ≈20 μL fetal bovine serum plus 50% sucrose (1:1), at which time the mixture was ejected into 250 μL mosquito diluent. Mosquito bodies were then placed in individual tubes with mosquito diluent. All samples were held at −80°C until tested. To test for infection, dissemination, and transmission, we processed and screened bodies, legs, and salivary secretions, respectively, by Zika virus–specific quantitative reverse transcription PCR (25 (link)). Zika virus body titers were calculated from standard curves based on infectious particle standards created from matched virus stocks. Data were analyzed by using GraphPad Prism version 4.0. Rates were compared by using Fisher exact tests, and dose dependence was evaluated and compared by using linear regression analyses.
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Publication 2017
Arboviruses ARID1A protein, human Blood Capillaries Cloning Vectors Culicidae Females Fetal Bovine Serum Fungizone Gentamicin Hemic System Human Body Infection Leg Penicillins Phosphates Pigs prisma Reverse Transcription Saline Solution Salivation Serum Sheep Streptomycin Sucrose Technique, Dilution Tissue, Membrane Transmission, Communicable Disease Virus Workshops Zika Virus
The data and protocols described in this manuscript are compliant with the minimum information about a microarray experiment (MIAME) and are deposited in the National Center for Biotechnology Information Gene Expression Omnibus (GEO) under accession number GSE13904 (GEO, ). All of the controls and 67 of the patients with septic shock have been previously reported in analyses addressing completely different questions than that addressed in the current study [9 (link),11 (link)-13 (link)]. An additional 31 patients in the septic shock cohort have not been previously reported. Total RNA was isolated from whole blood samples using the PaxGene™ blood RNA system (PreAnalytiX, Qiagen/Becton Dickson, Valencia, CA, USA) according the manufacturer's specifications. Microarray hybridization was performed by the Affymetrix Gene Chip Core facility at the Cincinnati Children's Hospital Research Foundation as previously described using the Human Genome U133 Plus 2.0 GeneChip (Affymetrix, Santa Clara, CA, USA) [9 (link),11 (link)-13 (link)].
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Publication 2009
AH 31 BLOOD Crossbreeding Gene Chips Gene Expression Genome, Human Hemic System Microarray Analysis Patients Septic Shock
Female NMRI or C57BL/6 mice were used. Freshly purified DiR-labelled EVs were injected through the tail vein for intravenous (i.v.) injections for most experiments. Intraperitoneal (i.p.) and subcutaneous (s.c.) injections were carried out as well, where indicated. Biodistribution of DiR-labelled EVs was examined using 3 different doses: 1.5×1010 particles/gram body weight (p/g), 1.0×1010 p/g and 0.25×1010 p/g, the particle count was measured with NTA and the samples were diluted accordingly. For analysis of DiR-EVs distribution, IVIS Spectrum (Perkin Elmer) was used. IVIS spectrum is an instrument that contains a high-sensitive CCD camera, which enables both fluorescence and luminescence measurements. Here, either live (isoflurane sedated) mice were imaged or the animals were sacrificed and the organs harvested prior to analysis. For the perfusion experiment, the mice were sedated and the vascular system was flushed by transcardial perfusion for 5 minutes. The left ventricle was infused with PBS (5 ml/min) and the right atrium was perforated. The outflow liquid, liver and tail were monitored during the procedure to assure successful perfusion. After 5 minutes of perfusion, the organs were harvested and analysed. The live mice or the harvested organs were imaged for 1–2 seconds (excitation 710 nm, emission 760 nm). The data were analysed with the IVIS software (Living Image Software for IVIS®). For fluorescent micro computed tomography (µCT)-images, mice were secured in a mouse imaging shuttle, imaged in the IVIS for 3D fluorescence (FLIT) and transferred to the Quantum FX micro CT Imaging System (PerklinElmer, MA, USA) for CT-imaging. Co-registration of FLIT with the CT-scan was generated using Living Image software (30 (link),31 (link)). Adobe Photoshop CS4 and Adobe Illustrator were used to crop out and align the organ images.
Injection of CD63-EGFP EVs: CD63-EGFP EVs were generated, isolated and characterized by NanoSight as described above. Twenty four hours post-injections (2.9×1010 p/g), the organs were harvested and prepared as described below. The animal experiments were approved by the British and the Swedish local boards for laboratory animals.
Publication 2015
Animals Animals, Laboratory Atrium, Right Body Weight Crop, Avian Fluorescence Hemic System Isoflurane Left Ventricles Liver Luminescent Measurements Magnetic Resonance Imaging Mice, House Mice, Inbred C57BL Neoplasm Metastasis Perfusion Tail Veins Woman X-Ray Computed Tomography X-Ray Microtomography
After purification and refolding, wild-type, K307N and N394K DIII were diluted (5 μg/ml) in 0.1 M Na carbonate buffer (pH 9.3) and adsorbed to 96-well plates overnight at 4 °C. After blocking with PBS, 2% BSA and 0.05% Tween 20 (PBS-BT), wells were preincubated for 1 h at 23 °C with PBS-BT containing no antibody, E16 IgG (50 μg/ml), E16 Fab (50 μg/ml) or E53 IgG (50 μg/ml). E53 serves as a negative control as it recognizes an epitope in domain I and II of WNV E protein. Subsequently, human plasma (1/40 dilution in PBS-BT, heat-inactivated) was directly added for an additional 1 h at 23 °C. We obtained the human samples with informed consent from seven different WNV-infected patients (gift of M. Busch and L. Tobler, Blood Systems Research Institute, San Francisco, CA). Because the samples were sequentially numbered and not linkable back to the original subjects, they satisfied the criteria for exemption from approval from the Human Studies Committee at Washington University. After six washes with PBS-BT, plates were serially incubated with biotin-conjugated goat anti-human IgG (1 μg/ml), streptavidin–horseradish peroxidase (2 μg/ml) and tetramethyl-benzidine developing substrate (DAKO). We determined optical densities at 450 nm with an automatic ELISA plate reader (Tecan) and adjusted them after subtraction of the value obtained from nonimmune human plasma.
Publication 2005
3,3',5,5'-tetramethylbenzidine Anti-Antibodies anti-IgG Biotin Buffers Carbonates Enzyme-Linked Immunosorbent Assay Epitopes Goat Hemic System Homo sapiens Horseradish Peroxidase Immunoglobulins Patients Plasma Proteins Streptavidin Technique, Dilution Tween 20 Vision

Most recents protocols related to «Hemic System»

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Example 1

Whole blood were collected from volunteer donors must be performed by personal trained in phlebotomy/venipuncture using a double blood bag system (about 50 ml) (TerumoBCT, Japan) with anticoagulant (1 ml of Anticoagulant Citrate Dextrose (ACD) Solution Formula/per 10 ml of blood). After blood collection, gently mix the blood by inverting the tube several times to ensure thorough mixing with anticoagulant. For thorough mixing of blood collected into citrate tubes, it is recommended to invert the tube 3-4 times, while ACD tubes should be inverted eight times. Blood samples should be maintained at temperate conditions (20-24° C.) and centrifuged within 4 hours of blood collection. To separate the plasma, centrifuge the blood samples at 1200×g for 10 minutes at 22° C. If needed, RCF for a centrifuge can be calculated. After centrifugation, the plasma layer will be the upper layer of the separated blood and appear a clear, straw-yellow colored fluid.

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Patent 2024
Anticoagulants BLOOD Centrifugation Citrates Donors Glucose Hemic System isolation Phlebotomy Plasma Voluntary Workers
All patients routinely received perianal screening for CRE within 48 hours of each hospital admission. In addition, some patients received perianal bacterial culture tests when they were suspected of infection by a competent physician during hospitalization. Perianal skin and throat swab samples were collected and submitted for examination by specially trained medical staff. Bacterial culture, identification and drug sensitivity test were conducted by special technicians in the microbiology laboratory, and the target bacteria were CRE. All CRE strains were isolated from perianal skin swabs and blood samples. Blood culture was performed using an automatic blood culture system (BD, USA). The isolation and identification of bacteria were carried out strictly following the relevant provisions of the National Clinical Laboratory Procedures. VITEK 2 compact (bioMérieux, France) was used to identify the isolates and MALDI-TOF MS (bioMérieux, France) was used for further confirmation. Antibiotic susceptibility testing was performed in the microbiology laboratory of the hospital using an automated system (VITEK 2 Compact) with the broth microdilution and disk diffusion methods. The following antibiotics were tested: penicillins (ticarcillin, piperacillin), β-lactamase inhibitor combinations (amoxicillin/clavulanic acid, piperacillin/tazobactam, cefoperazone/sulbactam), cephalosporins (cefazolin, cefuroxime, ceftazidime, cefepime, cefotaxime, cefotetan, cefpodoxime, ceftizoxime), quinolones (levofloxacin, moxifloxacin, ciprofloxacin, norfloxacin), carbapenems (imipenem, meropenem, doripenem), aminoglycosides (amikacin, tobramycin), tetracyclines (tetracycline, minocycline), aztreonam, trimethoprim/sulfamethoxazole and tigecycline. The minimum inhibitory concentration (MIC) was measured according to the guidelines of the 31st Edition of the Clinical and Laboratory Standards Institute (CLSI) M100-Performance Standards for Antimicrobial Susceptibility Testing.14 The detection of carbapenemases in CRE according to the modified carbapenem inactivation assay (mCIM and eCIM) provided by the CLSI 31th Edition.
Publication 2023
Amikacin Aminoglycosides Amox clav Antibiotics Aztreonam Bacteria beta-Lactamase Inhibitors Biological Assay Blood Blood Culture carbapenemase Carbapenems Cefazolin Cefepime Cefoperazone Cefotaxime Cefotetan cefpodoxime Ceftazidime Ceftizoxime Cefuroxime Cephalosporins Ciprofloxacin Clinical Laboratory Services Clinical Laboratory Techniques Diffusion Doripenem Hemic System Hospitalization Hypersensitivity Imipenem Infection isolation Levofloxacin Medical Staff Meropenem Microbicides Minimum Inhibitory Concentration Minocycline Moxifloxacin Norfloxacin Patients Penicillins Pharynx Physicians Piperacillin Piperacillin-Tazobactam Combination Product Quinolones Skin Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Strains Substance Abuse Detection Sulbactam Susceptibility, Disease Tetracycline Tetracyclines Ticarcillin Tigecycline Tobramycin Trimethoprim-Sulfamethoxazole Combination
Four-limb blood pressure and ABI measurement was performed by trained technicians using a non-invasive vascular profiling system (Omron VP-1000 vascular profiling system, Japan) [3 (link)]. This system ensured accurate and reliable ABI measurement using advanced oscillometric technology. Simultaneous blood pressure measurement at all four limbs was included, using a dual chamber cuff system and a proprietary algorithm. Measurement was performed after a 10-min rest in the supine position with the upper body as flat as possible. The device simultaneously and automatically measured the blood pressures twice, and then we calculated the means to get final blood pressure values. Bilateral ankle and brachial artery pressures, and bilateral ABI were supplied after measurement. ACC/AHA guidelines recommend ABI ≤ 0.90 as the criterion for the diagnosis of lower extremity PAD [8 (link)]. Meanwhile, IABPD ≥ 15 mmHg was considered as the potential abnormalities of upper extremity arteries according to literatures in this study [9 (link), 10 (link)].
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Publication 2023
Ankle Arteries Blood Pressure Brachial Artery Congenital Abnormality Determination, Blood Pressure Diagnosis Hemic System Human Body Lower Extremity Medical Devices Oscillometry Upper Extremity
This retrospective study used data from a microbiology database to describe BSI incidence from 1 January 2006 to 31 December 2019 in Skåne, southern Sweden, a region with ca 1.4 million inhabitants, see Supplementary material S1 for a description of geography and healthcare in the Skåne region. There is only one database for microbiological diagnostics in the region, which is at the Department of Clinical Microbiology in Lund. During the study period, local routine stated that two sets of blood cultures should be drawn from two separate venepunctures upon suspecting BSI. In the Skåne region, blood cultures are exclusively taken at hospitals providing secondary and tertiary healthcare, at emergency departments, inpatient wards or (rarely) in hospital-based outpatient care. At emergency departments, a nurse may obtain initial blood samples, including blood cultures, if suspecting BSI during triage. Whether the cultures are to be sent for analysis or not is then decided by the treating physician. The BacT/ALERT blood culture system (bioMérieux, Inc., Marcy-l’Étoile, France) was used in the Skåne region until December 2014, when it was replaced by the BACTEC FX (BectonDickinson, Franklin Lakes, United States). Susceptibility testing was performed by disk diffusion according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) standards [11 ].
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Publication 2023
Blood Blood Culture Care, Ambulatory Diagnosis Diffusion Europeans Hemic System Inpatient Microbicides Nurses Physicians Susceptibility, Disease Tertiary Healthcare
SBP was monitored for all animals at the start of the experiment,
second,, fourth, and sixth weeks using a tail-cuff blood pressure
measuring system (Harvard Apparatus Ltd, Edenbridge, Kent, England).13 (link)
Publication 2023
Animals Hemic System Tail

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The BacT/ALERT 3D is an automated microbial detection system designed for the rapid identification of microorganisms in blood cultures and other clinical specimens. The system utilizes colorimetric sensors to monitor the growth of microorganisms, providing a convenient and efficient means of detecting the presence of bacteria and fungi in patient samples.
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More about "Hemic System"

The Hemic System: A Vital Physiological Network for Blood, Immunity, and Homeostasis The Hemic System, also known as the Hematomic or Hematopoietic System, is a complex and dynamic network of cells, tissues, and organs responsible for the production, transport, and regulation of blood and its various components.
This essential system plays a crucial role in the body's immune response, circulation, and overall homeostasis.
At the core of the Hemic System are the bone marrow, spleen, lymph nodes, and the vast network of blood vessels that carry blood throughout the body.
These components work in harmony to deliver oxygen, nutrients, and other vital substances, while also removing waste products and maintaining the delicate balance required for optimal health.
Researchers studying the Hemic System often employ a variety of analytical techniques, including cutting-edge AI-driven analysis, to optimize protocols, compare products, and identify the best solutions for their research needs.
This approach can enhance the accuracy and reproducibility of Hemic System research, leading to a deeper understanding of this critical physiological system.
The Hemic System's importance is underscored by its involvement in numerous related processes, such as the RelaxGene Blood DNA System, CODA non-invasive blood pressure system, Contour Blood Glucose Monitoring System, and the ReliaPrep™ Blood gDNA Miniprep System.
These specialized tools and technologies provide valuable insights and support for researchers and clinicians working to unravel the complexities of the Hemic System.
Additionally, the BACTEC FX, BacT/ALERT 3D, and BACTEC FX blood culture systems play a crucial role in the detection and identification of microorganisms in the bloodstream, which is essential for understanding the Hemic System's immune function.
The CODA tail-cuff blood pressure system and the VaSera VS-1000 further contribute to the comprehensive study of the Hemic System's cardiovascular and circulatory aspects.
By leveraging the power of AI-driven analysis and a deep understanding of the Hemic System's intricate workings, researchers can optimize their protocols, compare products, and identify the best solutions to advance their studies.
This approach can lead to enhanced accuracy, improved reproducibility, and a more comprehensive understanding of this vital physiological network.