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> Anatomy > Body System > Lymphatic System

Lymphatic System

The lymphatic system is a complex network of vessels, tissues, and organs that play a crucial role in the body's immune response and fluid balance.
This system is responsible for transporting lymph, a fluid containing white blood cells, throughout the body, helping to fight infection and remove waste.
The lymphatic system includes the lymph nodes, spleen, thymus, and bone marrow, as well as the lymphatic vessels that connect these structures.
Proper function of the lymphatic system is essential for maintaining overall health and well-being.
Reasrchers can explore the latest advancements in lymphatic system research using AI-driven optimization tools like PubCompare.ai to find the most reliable and effective solutions for their studies.

Most cited protocols related to «Lymphatic System»

1
In vivo Visualization of Collecting Lymphatic Vessels

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Publication 2013
Blood Vessel Fistula Fluorescein-5-isothiocyanate Gossypium Gravity Hypersensitivity Intradermal Injection Ketamine Light Liposomes Lower Extremity Lymphatic System Medetomidine Mice, House Skin Vessel, Lymphatic
2
Tumor Microenvironment Drug Transport Modeling
Details of the mathematical model can be found in the appendix. Briefly, a Krogh cylinder geometry was used where a cylindrical blood vessel segment is surrounded by tissue with a radius approximately equal to half the local inter-capillary distance (Figure 1B). Blood flows from the arterial end, where a systemic two-compartment model with biexponential decay defines the concentration. The local blood concentration is determined by the blood velocity, permeability of the vessel wall, and fraction of free drug (not bound to blood cells or plasma proteins). Cellular uptake in the blood was ignored (File S1) given the slower kinetics relative to blood flow [22] (link). A mixed boundary condition is used at the capillary interface, where the flux at the capillary wall determined by the permeability is equal to the diffusive flux into the tissue. In the tissue, the free drug undergoes radial and axial diffusion along with agent specific reaction terms. For small molecules, this involves cellular uptake and metabolism (e.g. oxygen utilization, irreversible trapping over short time scales by FDG phosphorylation, reversible uptake for doxorubicin). For antibodies, this involves reversible binding and dissociation with irreversible internalization. Due to the lack of functional lymphatics in tumors, lymphatic drainage was ignored [23] (link). The following equations defined the plasma concentration, plasma tissue interface, and tissue concentration (for first order kinetics):

where [C]plasma is the total concentration of drug in the plasma, t is time, v is the local blood velocity, L is the length along the vessel segment, Rcap is the capillary radius, H is the hematocrit, P is the vessel wall permeability, ffree is the fraction of drug that is unbound, [C]tissue,free is the unbound concentration in the tissue (overall/pseudohomogenous concentration), and epsilon the void fraction. D is the effective diffusion coefficient in tissue, r is the radial distance from a vessel, and krxn defines the local reaction rate (which is first order in this example equation).
The method of lines was used with axial and radial variations and solved with a stiff solver using Matlab (The Mathworks; Natick, MA). A sparse Jacobian was defined to decrease simulation times.
Thurber G.M, & Weissleder R. (2011). A Systems Approach for Tumor Pharmacokinetics. PLoS ONE, 6(9), e24696.
Publication 2011
Antibodies Arteries BLOOD Blood Cells Blood Circulation Blood Vessel Capillaries Cells Diffusion Doxorubicin Drainage Kinetics Lymphatic System Metabolism Neoplasms Oxygen Permeability Pharmaceutical Preparations Phosphorylation Plasma Plasma Proteins Radius Tissues Urination Vascular Permeability Volumes, Packed Erythrocyte
3
Near-infrared Lymphatic Imaging in Mice
A Spy1000 system (Novadaq Technologies Inc Mississauga, Ontario, Canada) was used. This system consists of an 806nm laser providing maximum illumination intensity approximately 30 mW/cm2 at a fixed focal length. This instrument was modified to provide continuous operation and adjustment of laser intensity, and was equipped with a second PAL camera. Filters permitted the measurement of light above 815nm. The video outputs of the camera were attached to the network using an Axis 241SA video server (Axis, Sweden, Lund) that was monitored using SecuritySpy (Ben Bird, www.bensoftware.com) that converted motion JPEG image streams to QuickTime movies (Apple Computer Inc). Individual JPEG image sequences were then exported and read for further analysis with ImageJ.
ICG (Acorn or Pulsion) was dissolved in the distilled water at 0.1 μg/μl and 10 μl (1 μg) of ICG solution was intra-dermally injected into the footpad using a 30G needle. Before ICG injection, fur was removed from legs with hair removal lotion. Animals were placed in recumbent position and legs were restrained with tape on an isothermal gel pad during imaging sessions. ICG fluorescence was recorded for 1–2 hours immediately and again 24 hours for 5 min after the ICG injection. The QuickTime movies were examined and the time for ICG appearance in popliteal lymph node (PLNs) after the footpad injection was recorded. Sequential images from the movie file were exported and the intensity of ICG fluorescence of PLNs and footpads were determined using ImageJ. A fixed circular region of interest (ROI) was drawn over the vessels or PLNs and nearby background tissue. The signal intensity was defined as Signal intensity – background intensity. The following outcome measures were derived from the ICG-NIR images: 1) T-initial (T-in), which is the time that it takes for the ICG to be detected in vessels; 2) S-max, which is the maximum ICG signal intensity observed in the PLN during the first day imaging session; 3) T-max, which is the time it takes for a PLN to achieve maximal ICG signal intensity; 4) % Clearance, which is an assessment of ICG wash out through the lymphatics and is quantified as the percent difference of ICG signal intensity between the two ICG-NIR images from the ROI of the PLNs or footpad at a) S-max (first day) and b) 24 hours post ICG injection; and 5) pulse, which is the ICG pulses that pass the ROI within 400 seconds.
Zhou Q., Wood R., Schwarz E.M., Wang Y.J, & Xing L. (2010). Near infrared lymphatic imaging demonstrates the dynamics of lymph flow and lymphangiogenesis during the acute vs. chronic phases of arthritis in mice. Arthritis and rheumatism, 62(7), 1881-1889.
Publication 2010
Animals Aves Blood Vessel Depilation Epistropheus Fluorescence Leg Light Lymphatic System Needles Neoplasm Metastasis Nodes, Lymph Pulse Rate Tissues
4
Quantifying Metastatic Cancer Cells
The first step of metastatic spread involves movement of cancer cells from the primary site into the bloodstream (intravasation) either directly or indirectly through the lymphatics (17 (link)). Measurement of MDA-MB-231/eGFP cells in the blood of tumor-bearing SCID mice was determined by three methods in two separate experiments. In one experiment (113007), untreated (n = 3) and 200 mmol/L bicarbonate–treated (n = 7) animals bearing primary tumors were euthanized after 36 d of tumor growth. At this time point, the primary tumors averaged 463 ± 33.5 mm3 in size in both groups. Blood was extracted by cardiac puncture into microfuge tubes and mixed with an equal volume of 100 mmol/L EDTA to prevent clotting. A blood volume of 10 μL was smeared on glass slides and dried. Green-fluorescing cells were counted manually under a fluorescent microscope at ×40 magnification. Nucleated cells from the remaining blood volume (∼ 300 μL) were obtained by centrifugation with Histopaque (Sigma), and resulting cells were resuspended in 96-well plates in 100 μL of PBS and measured on a Victor3 with excitation wavelength at 485 nm and emission at 535 nm. In another experiment (011508), blood was extracted by heart puncture from untreated and bicarbonate-treated mice (n = 8 each) by the same methods as above. Average tumor size was 121.8 ± 16.4 mm3. RBC were lysed with fluorescence-activated cell sorting lysing solution (BD Sciences) according to the manufacturer's instructions. Cells were counter-labeled with LDS-751 nucleic acid dye and analyzed by flow cytometry on a FACScan (BD Biosciences) with a 488-nm argon laser. LDS-751 emits at 670 nm upon excitation at 488 nm and is detectable with the fluorescence 3 detector. Nonspecific fluorescence was differentiated from the green fluorescent protein (GFP) signal by gating on cellular light scattering properties and LDS-751.
Robey I.F., Baggett B.K., Kirkpatrick N.D., Roe D.J., Dosescu J., Sloane B.F., Hashim A.I., Morse D.L., Raghunand N., Gatenby R.A, & Gillies R.J. (2009). Bicarbonate Increases Tumor pH and Inhibits Spontaneous Metastases. Cancer research, 69(6), 2260-2268.
Publication 2009
Animals Argon Ion Lasers Bicarbonates BLOOD Blood Cells Blood Circulation Blood Volume Cells Centrifugation Edetic Acid Enzyme Multiplied Immunoassay Technique Flow Cytometry Fluorescence Green Fluorescent Proteins Heart histopaque LDS-751 Lymphatic System Malignant Neoplasms MDA-MB-231 Cells Microscopy Motility, Cell Mus Neoplasms Nucleic Acids Punctures SCID Mice TNFSF14 protein, human
5
Promoting Lymph Flow and Optimal BMI
The-Optimal-Lymph-Flow is a patient-centered educational and behavioral program focusing on self-care risk reduction strategies to promote lymph flow and optimize BMI by targeting known lymphedma risk of compromised lymphatic system and high BMI. Easy-to-learn self-care strategies include shoulder mobility exercises to promote shoulder function, muscle-tightening-breathing, muscle-tightening-pumping exercises, and large muscle exercises to promote lymph flow and drainage, as well as general instructions to encourage nutrition-balanced (more vegetables and fruits) and portion-appropriate diet (feeling 75% full for each meal) to strive for maintaining pre-operative BMI. Table 1 describes the self-care strategies and physiological rationales. Trained nurses delivered the intervention during a 30-minute face-to-face meeting with each patient. Patients demonstrated back the shoulder exercises, muscle-tightening-breathing, and muscle-tightening-pumping exercises.
Fu M.R., Axelrod D., Guth A.A., Cartwright F., Qiu Z., Goldberg J.D., Kim J., Scagliola J., Kleinman R, & Haber J. (2014). Proactive Approach to Lymphedema Risk Reduction: A Prospective Study. Annals of surgical oncology, 21(11), 3481-3489.
Publication 2014
Diet Drainage Education of Patients Face Fruit Lymph Lymphatic System Muscle Tissue Nurses Patients physiology Range of Motion, Articular Shoulder Vegetables

Most recents protocols related to «Lymphatic System»

1
Canine Contrast-Enhanced CT and ICTL
All dogs were anesthetized through the anesthesia service overseen by a board-certified anesthesiologist. All dogs were positioned for CT in sternal recumbency, with the head positioned on a 3D printed bite block (Fig 1) to elevate the oral cavity and limit pressure on draining lymphatics. CT images were acquired using a helical 64-slice scanner (Aquilion 64 CFX, Toshiba Medical Systems, Tustin, CA with maximum field of view of 70 cm) with the following parameters suitable to dog size: 120kVp, 40-80mA, 20-100mAs, rotation time 0.5 seconds. Intravenous contrast medium (770 mg of Iodine/kg [2 mL/kg]; Optiray 350 [Ioversol], Mallinckrodt Inc, Hazelwood, MO) was administered intravenously to all dogs following acquisition of pre-contrast data. All examinations consisted of reconstructed 2- or 3-mm thick transverse images and datasets were stored to allow analysis.
After post-contrast scan, ICTL was performed as previously described [36 (link),37 (link),39 (link)] with minor modifications. Briefly, 1 ml of Ioversol was diluted with 1 ml of saline. Four site peritumoral injections (0.5 ml per site) were performed with a 23-gauge butterfly catheter. Each injection was performed over 30 seconds followed by massage of the region. Scans were acquired 3 minutes (min) and 6 min from the start of the peritumoral injection. If no SLN was noted on the 6-min scan, an additional 1 ml of undiluted Ioversol was injected peritumorally in the same fashion. A repeat scan was performed 3 min later, at 12 min from the initial injection. Complications associated with the ICTL procedure were recorded.
Goldschmidt S., Stewart N., Ober C., Bell C., Wolf-Ringwall A., Kent M, & Lawrence J. (2023). Contrast-enhanced and indirect computed tomography lymphangiography accurately identifies the cervical lymphocenter at risk for metastasis in pet dogs with spontaneously occurring oral neoplasia. PLOS ONE, 18(3), e0282500.
Publication 2023
Anesthesia Anesthesiologist Butterflies Canis familiaris Cardiac Arrest Catheters Head Helix (Snails) Iodine ioversol Lymphatic System Massage Neoplasm Metastasis Optiray Oral Cavity Physical Examination Pressure Radionuclide Imaging Saline Solution Sternum
2
Indocyanine Green Cervical Mapping
Briefly, 25 mg of ICG were diluted in 20 cc of sterile water in order to obtain a concentration of 1.25 mg/mL. Just before starting surgery, we performed a slow superficial (1–3 mm) and deep (1–2 cm) infiltration of 1 cc of ICG solution at 3 and 9 o’clock positions on the cervix with a spinal needle (20 G—90 mm). With this technique, the ICG was injected in the submucosal space and in the cervical stroma, near the blood vessels, an area with a rich lymphatic drainage. To see the diffusion of ICG, we used specific laparoscopic equipment: an optic with an infrared filter, a light source, and a high-definition camera. The diffusion of ICG can be divided in three phases: the first, which lasts a few seconds and shows the site of inoculation and the parametrium; the second (20–30 min later), in which the lymphatic system draining the site of inoculum is visible; and the third (1–2 h later), called the vascular phase, when the ICG goes in the vascular circulation, with a partial contamination of the surgical field. The best phase to perform the lymphadenectomy is the second one.
All patients underwent pelvic lymphadenectomy (with or without ICG), with complete removal of external and internal iliac lymph nodes up to the level of common iliac bifurcation and obturator lymph nodes. During procedures with ICG, a careful identification and isolation of lymphatic vessels and mapped lymph nodes was performed to avoid the extravasation of the ICG.
Chiofalo B., Laganà A.S., Ghezzi F., Certelli C., Casarin J., Bruno V., Sperduti I., Chiantera V., Peitsidis P, & Vizza E. (2023). Beyond Sentinel Lymph Node: Outcomes of Indocyanine Green-Guided Pelvic Lymphadenectomy in Endometrial and Cervical Cancer. International Journal of Environmental Research and Public Health, 20(4), 3476.
Publication 2023
Blood Vessel Cervix Uteri Diffusion Drainage Eye Ilium isolation Laparoscopy Light Lymphatic System Lymph Node Excision Neck Needles Nodes, Lymph Operative Surgical Procedures Parametrium Patients Pelvis Sterility, Reproductive Vaccination Vessel, Lymphatic
3
Lymphedema and Wound Healing in Rats
The study was approved by the Institutional Animal Care and Use Committee of Taipei Veterans General Hospital. All animal care complied with the Guide for the Care and Use of Laboratory Animals (No. IACUC 2019-108, IACUC 2020-155, IACUC 2020-240). Fifteen male Sprague Dawley rats ((BioLASCO, Yilan, Taiwan)) aged 20 to 24 weeks were used for this research. Animals were divided into three groups randomly, which were as follows: (i) secondary healing (control) (n = 5); (ii) decellularized Wharton’s jelly (dWJ) (n = 5); and (iii) ASC-decellularized Wharton’s jelly (ASC/dWJ) (n = 5). Sprague Dawley rats were anesthetized with an intraperitoneal injection of 50 mg/kg body weight of Zoletil 50 (Virbac, Carros cedex, France). The anesthetized rats were placed in a prone position over a warm pad. The diameter of the tail and the excision wound was calculated by a Vernier scale. Evan’s blue dye (E2129, Sigma-Aldrich, St. Louis, MO, USA) was injected into the tail base before operation in order to identify the lymph vessels. After shaving and sterilization, a circumferential excision (1 cm in width) of skin at a 5 mm distance from the base of the tail was made (Figure 1A). The superficial lymphatic network was removed, and the deep lymphatic system and lateral tail veins were preserved (Figure 1B). The rats recovered from anesthesia in a separate cage after the operation and received food and water ad libitum.
Tail volume and wound width for each group were evaluated to track tail lymphedema and wound-healing. The data for each group were compared from week 1 to week 5 after surgery. The tail volumes were calculated using a truncated cone formula according to previously published methods (Figure 1C) [40 (link)]. For this purpose, tail diameter at 2 cm and 16 cm from the tail’s end were measured by two blinded investigators using a digital caliper. The increase in volume, which represents lymphedema, was defined as post- vs. pre-operative volumes of the same animal for each week.
The digital images of the wound were captured weekly in order to quantify the wound width. Width was determined by the average of three locations (the edge of the wound on both sides and the center of the wound). The values were measured from digital pictures using ImageJ software (version 1.53t).
Lu J.H., Hsia K., Su C.K., Pan Y.H., Ma H., Chiou S.H, & Lin C.H. (2023). A Novel Dressing Composed of Adipose Stem Cells and Decellularized Wharton’s Jelly Facilitated Wound Healing and Relieved Lymphedema by Enhancing Angiogenesis and Lymphangiogenesis in a Rat Model. Journal of Functional Biomaterials, 14(2), 104.
Publication 2023
Anesthesia Animals Animals, Laboratory Body Weight Cedax Evans Blue Finger Injuries Fingers Food Injections, Intraperitoneal Institutional Animal Care and Use Committees Lymphatic System Lymphedema Males Operative Surgical Procedures Rats, Sprague-Dawley Rattus norvegicus Retinal Cone Skin Sterilization, Reproductive Tail Veins Vessel, Lymphatic Wharton Jelly Wounds Zoletil
4
Lymphatic Metastasis Dynamics of Breast Cancer
The lymphatic system is an extensive vascular network that can be considered the primary way to spread metastatic breast cancer cells (BCCs). The dynamics by which BCCs travel to distant sites in the lymph nodes has just been well understood. Particle tracking techniques were used to analyze the behavior of BCCs and standard solid particles of different diameters that were used to simulate cell flow in the lymph. Distinct differences between BCC and particle behavior indicate that morphology and size affect their response to lymphatic flow conditions64 (link).
The BCCs adhered together and formed aggregate particles whose behavior was irregular. At the lymph flow rate, the MCF-7s were uniformly distributed across the channel compared to MDA-MB-231 cells that moved in the central region, indicating that metastatic MDA-MB-231 cells are subjected to a lower range of shear stresses in vivo. This suggests that size and deformability must be considered when modeling BCC behavior in the lymphatics. Human breast cell lines MDA-MB-231 (11–22 μm), MCF-7 (11–19 μm), and WBCs (6–16 μm), were used in this study64 (link),65 (link). Since about two-thirds of WBCs are in the range of 12–14 μm and about one-third of WBCs are in the range of 6–9 μm, the average diameter is assumed to be about 12 μm. Table 1 shows the characteristics of the cells used in this research; these cells were obtained from the Biotechnology Center of Tarbiat Modares University.

Characteristics of cells floating in the lymphatic system.

Cell typemcf-7 (μm)mda-mb-231 (μm)wbc (μm)
Min diameter11116
Max diameter192216
Average diameter151812
Omrani V., Targhi M.Z., Rahbarizadeh F, & Nosrati R. (2023). High-throughput isolation of cancer cells in spiral microchannel by changing the direction, magnitude and location of the maximum velocity. Scientific Reports, 13, 3213.
Publication 2023
Blood Vessel Breast Breast Carcinoma Cell Lines Cells Homo sapiens Lymph Lymphatic System Lymphocyte MDA-MB-231 Cells Neoplasm Metastasis Nodes, Lymph
5
RNA Isolation and Real-Time PCR Analysis
The total RNA was extracted from the cells treated as above using the PureLink™ RNA Mini Kit (Invitrogen, Waltham, MA, USA) following the manufacturer’s protocol. For RNA isolation from mice liver LNs, the tissues were rapidly snap frozen in liquid N2 immediately after their isolation and stored at −80 °C in a freezer until further processing. RNA isolation was done using a PureLink™ RNA Mini Kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s protocol. Briefly, snap frozen tissues were homogenized with an appropriate volume of RNA lysis buffer provided with the kit and homogenized by rotor-stator, in a chilled, 4 mL round bottom RNase-free tube for 30–40 s, followed by centrifugation at 2600× g for 5 min at room temperature. The supernantant was carefully transferred to a fresh RNase-free tube and proceeded to subsequnt binding, washing, and elution steps following the manufacturer’s protocol. The RNA quality and quantity were measured using NanoDrop Technologies (NanoDrop Technologies, Wilmington, DE, USA) and, subsequently, cDNA was prepared using the Maxima H Minus cDNA Synthesis Kit from Life Technologies (Carlsbad, CA, USA). Real-time PCR was performed for the genes for chemokines, cytokines, cellular stress, markers for the lymphatic system, and lymphangiogenesis using the PowerUp™ SYBR™ Green Master Mix (Applied Biosystems, Foster City, CA, USA) in the real-time thermal cycler (ABI Prism 7900HT sequence detection system; Applied Biosystems) and each reaction was performed in triplicate. The data analysis was conducted using the 2−ddCt method [29 (link)]. Ubiquitin/ribosomal protein L19 (RPL19) genes were used as the housekeeping genes for the data analysis. The sequences of the primers (Sigma Aldrich) used for the specific genes are listed in Table 1. Premade primers for FXR, TGR5, VDR were purchased from Sigma Aldrich.
Banerjee P., Kumaravel S., Roy S., Gaddam N., Odeh J., Bayless K.J., Glaser S, & Chakraborty S. (2023). Conjugated Bile Acids Promote Lymphangiogenesis by Modulation of the Reactive Oxygen Species–p90RSK–Vascular Endothelial Growth Factor Receptor 3 Pathway. Cells, 12(4), 526.
Publication 2023
2-5A-dependent ribonuclease Anabolism Buffers Cells Centrifugation Chemokine Cytokine DNA, Complementary Endoribonucleases Freezing Genes Genes, Housekeeping isolation Liver Lymphangiogenesis Lymphatic System Mus Oligonucleotide Primers prisma Real-Time Polymerase Chain Reaction Ribosomal Proteins SYBR Green I Tissues Ubiquitin

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1
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2
Isoflurane by Abbott
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Isoflurane is an inhaled anesthetic agent used to induce and maintain general anesthesia in medical and veterinary settings. It is a clear, colorless, and volatile liquid. Isoflurane functions as a potent and effective anesthetic by depressing the central nervous system, resulting in unconsciousness, analgesia, and muscle relaxation.
3
Bodipy fl c16 by Thermo Fisher Scientific
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BODIPY FL C16 is a fluorescent lipid analog used to label and track lipids in biological systems. It consists of a BODIPY fluorophore conjugated to a 16-carbon fatty acid chain. This product can be used to visualize lipid uptake, trafficking, and distribution in cells and tissues.
4
Hrp second antibody by Thermo Fisher Scientific
Sourced in United States
The HRP second antibody is a laboratory reagent used in immunoassays and other applications that require the detection and quantification of target molecules. It consists of a secondary antibody conjugated with the enzyme horseradish peroxidase (HRP), which catalyzes a colorimetric or chemiluminescent reaction for signal detection. The core function of the HRP second antibody is to bind to a primary antibody that has been used to capture the target analyte, thereby allowing for the visualization and quantification of the target through the HRP-catalyzed reaction.
5
Dab substrate liquid by Gene Tech
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DAB substrate liquid is a solution used in immunohistochemistry and immunocytochemistry techniques. It serves as a chromogenic substrate that enables the visualization of target proteins or antigens in biological samples.
6
Total nucleic isolation kit by Roche
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Lsm 780 by Zeiss
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The LSM 780 is a laser scanning microscope developed by Zeiss. It is designed for high-resolution imaging and analysis of biological samples. The instrument utilizes advanced confocal technology to provide detailed, three-dimensional images of specimens.
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Quantitect reverse transcription kit by Qiagen
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The QuantiTect Reverse Transcription Kit is a laboratory tool designed for the reverse transcription of RNA into complementary DNA (cDNA). It enables the conversion of RNA samples into cDNA, which can then be used for various downstream applications, such as real-time PCR or gene expression analysis.
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Alexa fluor 555 by Thermo Fisher Scientific
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Alexa Fluor 555 is a fluorescent dye used in various biological applications. It has an excitation maximum at 555 nm and an emission maximum at 565 nm, making it suitable for detection and labeling in a range of assays and imaging techniques.

More about "Lymphatic System"

The lymphatic system is a vital network of vessels, tissues, and organs that play a crucial role in the body's immune response and fluid balance.
Also known as the lymphoid system, this intricate network is responsible for transporting lymph, a fluid containing white blood cells, throughout the body to help fight infection and remove waste.
Key components of the lymphatic system include the lymph nodes, spleen, thymus, and bone marrow, as well as the lymphatic vessels that connect these structures.
Proper function of the lymphatic system is essential for maintaining overall health and well-being.
Researchers can explore the latest advancements in lymphatic system research using AI-driven optimization tools like PubCompare.ai to find the most reliable and effective solutions for their studies.
This cutting-edge platform allows researchers to compare literature, pre-prints, and patents, unlocking the power of the lymphatic system with AI-driven optimization for reproducible and accurate research.
When conducting lymphatic system studies, researchers may utilize various techniques and tools, such as Sylgard 184 (a silicone elastomer used for microfluidic device fabrication), Isoflurane (an anesthetic agent), BODIPY FL C16 (a fluorescent lipid probe), HRP second antibody (a secondary antibody conjugated with horseradish peroxidase), DAB substrate liquid (a chromogenic substrate for HRP), Total Nucleic Isolation Kit (for extracting DNA and RNA), LSM 780 (a confocal laser scanning microscope), QuantiTect Reverse Transcription Kit (for cDNA synthesis), and Alexa Fluor 555 (a fluorescent dye).
These specialized tools and techniques can provide valuable insights into the structure, function, and dynamics of the lymphatic system.
By incorporating the latest advancements and utilizing cutting-edge technologies, researchers can unlock new discoveries and advance our understanding of the lymphatic system, ultimately leading to improved healthcare solutions and better patient outcomes.