Cellular Structures
From organelles and membranes to the extracellular matrix, explore the intricate components that enable cellular function and facilitate communication within living organisms.
Understand the importance of cellular structures in biological procesess, and learn how researchers can optimize experimental protocols to enhnace reproducibility and accuracy when studying these fundamental elements of life.
Most cited protocols related to «Cellular Structures»
miRNA:gene interactions are derived from the in silico miRNA target prediction algorithms: DIANA-microT-CDS and TargetScan 6.2, the latter in both Context+ and Conservation modes. DIANA-microT-CDS is the fifth version of the microT algorithm (3 (link)). It is a highly accurate target prediction algorithm trained against CLIP-Seq datasets, enabling target prediction in 3′ UTR and CDS mRNA regions. The user of DIANA-miRPath v3.0 can also utilize experimentally supported interactions from DIANA-TarBase v.7.0. TarBase v7.0 incorporates more than half a million experimentally supported miRNA:gene interactions derived from hundreds of publications and more than 150 CLIP-Seq libraries (17 (link)). The number of indexed interactions is 9–250-fold higher compared to any other manually curated database. The user of miRPath v3.0 can harness this wealth of information and substitute or combine in silico predicted targets with high quality experimentally validated interactions. Currently, this functionality is supported for H. sapiens and M. musculus and C. elegans, since most relevant wet-lab experiments correspond to these species. As more experimental data become available for other organisms in DIANA-TarBase, the experimentally supported functional analysis module will be further extended.
New subcategory SCLs predicted by PSORTb 3.0
SCL subcategories | Description |
---|---|
Host-associated | Any proteins destined to the host cell cytoplasm, cell membrane or nucleus by any of the bacterial secretion systems |
Type III secretion | Components of the Type III secretion apparatus |
Fimbrial | Components of a bacterial or archaeal fimbrium or pilus |
Flagellar | Components of a bacterial or archaeal flagellum |
Spore | Components of a spore |
Most recents protocols related to «Cellular Structures»
Example 3
SLMs were first subjected to X-ray diffraction (XRD) to decipher any order arising due to self-assembly of cellular components. XRD spectra shown in
activities were 60 fourth-year university-level
students from Chemistry and Chemistry & Material Sciences areas.
They were separated into two groups that followed the same activities.
During the sessions, the 30 subjects shared the same classroom and
were instructed by the same teacher. A survey conducted at the beginning
of the semester showed that none of them had any prior experience
with PDB or PyMOL.
The activities were divided into three 2
h class sessions.
Session
1. The students were instructed
in the basic skills of PDB and PyMOL software required to visualize
and manipulate macromolecular structures. As an example for training,
the spike protein of SARS-CoV-2 was employed.
Session 2. PyMOL was used to manipulate
and explain the structure of the ACE2 receptor cell, along with its
complexes with the SARS-CoV-2 spike protein.
Session 3. Structural analysis of several
antibodies in complex with the spike protein were studied. Finally,
students were able to answer the question: how is it explained
chemically that vaccines save lives?
out
in which the students were requested to assess different aspects of
their experience.
The mRNA groups with functional significance in cancer were clustered with the MCODE plug-in, according to the following criteria: MCODE score > 5, degree cut-off = 2, node score cut-off = 0.2, maximum depth = 100, and K-core = 2. We studied the biological functions and signaling mechanisms of the top three DEM clusters. Gene Ontology (GO) annotation included three aspects: BP, molecular function, and cellular component. The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used to identify the possible pathways in which the molecule was involved, and the screening criterion was set at p < 0.05.
Top products related to «Cellular Structures»
More about "Cellular Structures"
These include organelles, membranes, the extracellular matrix, and other fundamental elements that enable cellular function and communication within living organisms.
Understanding the importance of cellular structures is crucial for biological processes and researchers can optimize experimental protocols to enhance reproducibility and accuracy when studying these essential elements of life.
Cellular structures include a wide range of components, such as the nucleus, mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, peroxisomes, and the cytoskeleton.
These structures play crucial roles in cellular activities like energy production, protein synthesis, and intracellular transport.
Additionally, the extracellular matrix provides structural support and facilitates cell-to-cell communication.
Researchers can utilize a variety of techniques and reagents to study cellular structures, such as fluorescence microscopy with DAPI staining, Triton X-100 for membrane permeabilization, and TRIzol reagent for RNA extraction.
Bioinformatics tools like Ingenuity Pathway Analysis (IPA) can also help researchers analyze the complex pathways and interactions involving cellular structures.
To enhance the reproducibility and accuracy of their experiments, researchers can optimize their protocols by comparing different methods and products, such as those found in the comprehensive database of PubCompare.ai.
This AI-driven platform enables researchers to identify the best protocols and products for their specific research needs, improving the efficiency and reliability of their experiments.
Whether you're studying organelle function, extracellular matrix dynamics, or any other aspect of cellular structures, understanding the key components and utilizing the right experimental tools and techniques can be a game-changer in your research.
Stay up-to-date with the latest advancements in this field and leverage the power of AI-driven platforms like PubCompare.ai to enhance the quality and impact of your work.