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> Anatomy > Cell Component > Nissl Bodies

Nissl Bodies

Nissl bodies are distinct granular structures found in the cytoplasm of neurons, composed of rough endoplasmic reticulum and free ribosomes.
They play a crucial role in protein synthesis and are often used as indicators of neuronal health and activity.
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Most cited protocols related to «Nissl Bodies»

1
Immunohistochemical Staining of Macrophages

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Publication 2009
Antibodies Antibody Specificity Biological Assay Calcium Cell Nucleus Cells Cloning Vectors Contusions cresyl violet Frozen Sections Immunoglobulins Immunoprecipitation ITGAM protein, human Macrophage Macrophage-1 Antigen Mus Myeloid Cells Nissl Bodies Proteins Protoplasm Ribosomal RNA Tissue, Membrane Tissues Tissue Stains Western Blotting Zymosan
2
Quantification of Ventricle and Lesion Volumes
On day 3 after ICH, five mice in each group were anesthetized and perfused intracardially with 4% paraformaldehyde in 0.1 mol/L phosphate-buffered saline (pH 7.4). The brains were removed, kept in 4% paraformaldehyde for 24 h, and then immersed in 30% sucrose for 72 h at 4°C. The entire brain of each mouse was cut into 50-μm-thick sections with a cryostat. All sections except for those from the IVH group were stained with Luxol fast blue (for myelin) and Cresyl violet (for Nissl bodies). Sections from the IVH group were stained only with Cresyl violet. SigmaScan Pro software (version 5.0.0 for Windows; Systat, Port Richmond, CA) was used to quantify volumes of the ventricle and lesion. The volume of the ventricles in cubic millimeters was calculated according to a published method [26] (link). The volume of the lesion in cubic millimeters was calculated by multiplying the thickness by the sum of the damaged areas of each section [14] (link). Sections were analyzed by an investigator blinded to the experimental cohort.
Zhu W., Gao Y., Chang C.F., Wan J.R., Zhu S.S, & Wang J. (2014). Mouse Models of Intracerebral Hemorrhage in Ventricle, Cortex, and Hippocampus by Injections of Autologous Blood or Collagenase. PLoS ONE, 9(5), e97423.
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Publication 2014
Brain Cerebral Ventricles cresyl violet Cuboid Bone Heart Ventricle Luxol Fast Blue MBS Mice, House Myelin Sheath Nissl Bodies paraform Phosphates Saline Solution Sucrose
3
High-Resolution MRI and Histology of Formalin-Fixed Hippocampus

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Publication 2013
Autopsy Axon Brain Cells cresyl violet ECHO protocol Epistropheus Face Formalin Hippocampal Formation Longterm Effects Luxol Fast Blue MBS Microtomy Myelin Sheath Neurons Nissl Bodies Paraffin Embedding perfluoropolyether Reconstructive Surgical Procedures Seahorses Susceptibility, Disease Tissues Viola Woman
4
Immunohistochemical Analysis of Spinal Cord
Dissected tissues were post-fixed in 4% paraformaldehyde/PBS or Carnoy’s fixative and embedded in paraffin wax. Eight micrometre thick sections were cut and immunostained as described previously (32 (link)) using Elite plus kits (Vector laboratories) and 3,3′-diaminobenzidine as a substrate. For double immunofluorescence, secondary Alexa Fluor-conjugated antibody (Invitrogen) were used. Images were prepared using laser scanning confocal microscope Leica TCS SP2 (Leica Microsystems) and LEICA CONFOCAL 2.00 software. For detection and counting of amyloid aggregates, spinal cord sections (four to five animals per age/genotype/condition group) were stained in 0.5% Congo red solution in 50% ethanol for 7 min followed by a brief differentiation in potassium hydroxide (0.2% in 80% ethanol) and, after several washes in dH2O, were mounted using Immu-mount (Thermo Electron Corporation). Fluorescent microscopy was used to analyse the number of aggregates within a randomly selected 0.1 mm2 area of the spinal cord gray matter. For assessing number of spinal motor neurons, transverse spinal cord sections were stained in 0.5% cresyl fast violet (CFV) solution in dH2O and dissociated in acidified ethanol (0.25% acetic acid in ethanol). The number of motor neurons (identified by a large cell body containing Nissl granules, with a clear nuclear envelope containing a strongly stained nucleolus) within the ventral horn was counted. Results were expressed as per cent of the average number of motor neurons per ventral horn section in the wild-type animals.
Ninkina N., Peters O., Millership S., Salem H., van der Putten H, & Buchman V.L. (2009). γ-Synucleinopathy: neurodegeneration associated with overexpression of the mouse protein. Human Molecular Genetics, 18(10), 1779-1794.
Publication 2009
Acetic Acid Amyloid Proteins Animal Diseases Animals, Wild Cell Body Cell Nucleolus Cloning Vectors Electrons Ethanol Fixatives Fluorescent Antibody Technique Genotype Gray Matter Horns Immunoglobulins Microscopy Microscopy, Confocal, Laser Scanning Motor Neurons Nile Blue Nissl Bodies Nuclear Envelope Paraffin paraform potassium hydroxide Spinal Cord Tissues
5
Histological Analysis of Viral Transduction in Mouse Brains

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Publication 2017
alexa 568 alexa fluor 488 Anesthesia anti-IgG Antibodies Antibodies, Anti-Idiotypic Brain Buffers Cerebellum Cloning Vectors Equus asinus Fluorescence glutamate decarboxylase 1 (brain, 67kDa), human Herpesvirus 1, Cercopithecine Immunoglobulins Left Ventricles Lentivirus Mice, House Mice, Transgenic Microscopy, Fluorescence Nissl Bodies paraform Parvalbumins Phosphates Rabbits Serum Serum Albumin, Bovine Sodium Azide Triton X-100

Most recents protocols related to «Nissl Bodies»

1
Nissl Staining of Mouse Hippocampus
The CA1, CA3 and DG regions of the mouse hippocampus was assessed by Nissl staining for Nissl body detection. Brain sections were dehydrated as the same as in HE staining, followed by 1% tar violet staining for 1 h, and with distilled water washing followed by 70% alcohol separation for 1 min. Tissues were then dehydrated in 70%, 80% and 90% of alcohol. After transillumination in dimethylbenzene, sections were sealed with neutral adhesive for microscopic observation. Images were captured by a light microscope (Leica, WZ, GER).
Song L.L., Qu Y.Q., Tang Y.P., Chen X., Lo H.H., Qu L.Q., Yun Y.X., Wong V.K., Zhang R.L., Wang H.M., Liu M.H., Zhang W., Zhang H.X., Chan J.T., Wang C.R., Wu J.H, & Law B.Y. (2023). Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice. Redox Biology, 61, 102637.
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Publication 2023
Brain Ethanol Light Microscopy Mice, House Microscopy Nissl Bodies Seahorses Tissues Transillumination Viola Xylene
2
Hippocampal Histology and Quantification
Hematoxylin and eosin (H&E) staining was applied to observe the basic structure of hippocampal tissue. The hippocampal paraffin sections (5 µm) were dewaxed with xylene and rehydrated with gradient alcohols. Then, the sections were stained with hematoxylin, differentiated with hydrochloric acid ethanol, and redyed with eosin. Finally, the sections were dehydrated with gradient alcohol, and vitrified with xylene.
Nissl staining was performed as previously described.16 (link) After deparaffinization, the sections were stained with 1% toluidine blue for 30 min at room temperature. Then, the sections were dehydrated, vitrified and sealed. The whole and enlarged hippocampus were visualized by electron microscopy (DM3000, Leica, Solms, Germany). The mean density of Nissl bodies was calculated by IPP Image-Pro Plus 6.0 software (IPP 6.0, Media Cybernetics, USA).
Yang X.Y., Geng L., Li R., Song J.X., Jia C.L., An J.R., Sun M.F., Xu S., Guo Y.J., Zhao Y, & Ji E.S. (2023). Huperzine A-Liposomes Efficiently Improve Neural Injury in the Hippocampus of Mice with Chronic Intermittent Hypoxia. International Journal of Nanomedicine, 18, 843-859.
Publication 2023
Alcohols Electron Microscopy Eosin Ethanol Hydrochloric acid Nissl Bodies Paraffin Seahorses Tissues Tolonium Chloride Xylene
3
Histological Analysis of Rat Brain Tissue
Rat brain tissues were fixed with 4% neutral formaldehyde for 48 h, then embedded in paraffin and cut into 3 μm-thick slices. Slices were dewaxed in xylene and dehydrated in graded ethanol. The slices were stained with Nissl (Solarbio, Beijing, China) dye solution. The changes of Nissl bodies and the pathological morphology of brain tissues were observed under light microscope (55i, Nikon, Japan).
Ji W., Ren Y., Wei X., Ding X., Dong Y, & Yuan B. (2023). Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway. Scientific Reports, 13, 2788.
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Publication 2023
Brain Ethanol Formaldehyde Light Microscopy Nissl Bodies Paraffin Embedding Tissues Xylene
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Nissl Staining for Neuronal Survival Assessment
Nissl staining was performed to assess neuronal survival after MCAO. Coronal cryosections with a thickness of 3 μm were stained with Nissl staining solution (C0117, Beyotime, Shanghai, China) at 37 °C for 5 min on day 3 after modeling. The samples were washed with 95% ethanol for 5 min and dried. The sections were then washed twice in xylene for 5 min each. After being sealed with resin, the cytoplasm was stained blue–purple (Nissl bodies), and the nuclei were stained light blue–purple when viewed under a light microscope. Normal neurons have relatively large cell bodies, are rich in cytoplasm and have a large number of Nissl bodies, with one or two large round nuclei, whereas damaged cells have shrunken cell bodies, condensed nuclei, reduced or no Nissl bodies, dark cytoplasm and many vacuoles.
Hu R., Liang J., Ding L., Zhang W., Wang Y., Zhang Y., Zhang D., Pei L., Liu X., Xia Z., Xu Y, & Song B. (2023). Gasdermin D inhibition ameliorates neutrophil mediated brain damage in acute ischemic stroke. Cell Death Discovery, 9, 50.
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Publication 2023
Cell Body Cell Nucleus Cells Cryoultramicrotomy Cytoplasm Ethanol Light Light Microscopy Neurons Nissl Bodies Resins, Plant Vacuole Xylene
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Nissl Staining of Hippocampal Neurons
Nissl staining was used to assess the damage to hippocampal neurons [50 (link)]. After dewaxing and dehydration, brain sections were stained in toluidine blue solution in a 56 °C thermostat for 40 min. The sections were then removed and placed in 75%, 80%, and 95% ethanol for 5 min, followed by 100% ethanol twice for 2 min and xylene twice for 5 min. The neutral resin was added dropwise above the tissues. Subsequently, these sections were observed under the microscope and photographed. The number of Nissl bodies in the hippocampus of each animal was calculated by Image J software.
Yang C.Z., Wang S.H., Zhang R.H., Lin J.H., Tian Y.H., Yang Y.Q., Liu J, & Ma Y.X. (2023). Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice. Cell Death Discovery, 9, 15.
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Publication 2023
Animals Brain Dehydration Ethanol Microscopy Neurons Nissl Bodies Resins, Plant Seahorses Tissues Tolonium Chloride Xylene

Top products related to «Nissl Bodies»

1
Nissl staining solution by Beyotime
Sourced in China, United States
Nissl staining solution is a laboratory reagent used to stain the Nissl substance in cells, particularly neurons. The Nissl substance is a collection of rough endoplasmic reticulum and free ribosomes within the cytoplasm of nerve cells. This staining solution allows for the visualization and identification of these structures under a microscope, which is important for various neuroanatomical and neurological studies.
2
Cresyl violet by Merck Group
Sourced in United States, Germany, United Kingdom, Switzerland, China, Japan, Belgium, Sao Tome and Principe, France, India
Cresyl violet is a histological stain used in microscopy to visualize cellular structures. It is a basic aniline dye that selectively binds to nucleic acids, staining the nuclei of cells. This allows for the identification and differentiation of various cell types in tissue samples.
3
Image pro plus 6 by Media Cybernetics
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Image-Pro Plus 6.0 is a comprehensive image analysis software package designed for scientific and industrial applications. It provides a wide range of tools for image capture, enhancement, measurement, analysis, and reporting.
4
C0117 by Beyotime
Sourced in China
The C0117 is a compact and versatile laboratory equipment designed for basic research and experimentation. It serves as a platform for various scientific applications. The core function of the C0117 is to provide a controlled environment for conducting experiments and analyses.
5
Nissl staining solution by Solarbio
Sourced in China
Nissl staining solution is a laboratory reagent used for staining the Nissl substance in cells, particularly nerve cells. It is a commonly used technique in histology and neuroscience to visualize the structure and distribution of neurons in tissue samples.
6
Cresyl violet acetate by Beyotime
Sourced in China
Cresyl violet acetate is a chemical compound commonly used in laboratory settings. It is a synthetic dye that has applications in various staining techniques, particularly in histology and microscopy. Cresyl violet acetate is known for its ability to stain specific cellular structures, which can aid in the visualization and analysis of biological samples.
7
Cv acetate by Merck Group
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CV acetate is a chemical compound used in various laboratory applications. It serves as a key component in the production of buffers and reagents utilized in scientific research and analytical procedures. The core function of CV acetate is to maintain pH levels and facilitate chemical reactions within controlled laboratory environments.
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Microtome by Leica
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A Microtome is a precision instrument used to cut extremely thin sections of material, typically for microscopic examination. It operates by moving a sample through a sharp blade, producing uniform slices of the desired thickness. The core function of a Microtome is to enable the preparation of high-quality samples for various microscopic techniques.
9
Nissl staining kit by Solarbio
Sourced in China
The Nissl staining kit is a set of reagents and solutions used to visualize the Nissl substance in cells, particularly in the nervous system. The kit provides the necessary components to perform the Nissl staining technique, which is a widely used histological method for the identification and analysis of neuronal cell bodies.
10
Nissl staining by Beyotime
Sourced in China
Nissl staining is a histological technique used to visualize the Nissl substance, which consists of rough endoplasmic reticulum and free ribosomes in the cytoplasm of neurons. This staining method is commonly used in neuroscience research to study the structure and organization of the nervous system.

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