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Astrocytes

Astrocytes are a type of glial cell found in the central nervous system.
They perform a variety of essential functions, including providing structural and metabolic support for neurons, regulating the blood-brain barrier, and modulating synaptic transmission.
Astrocytes play a crucial role in maintaining homeostasis and neuronal health within the brain and spinal cord.
Understanding the biology and function of astrocstes is key to advancing research into neurodegenerative diseases, brain injury, and other neurological conditions.
The PubCompare.ai platform offers a powerful AI-driven tool to help researchers optimize their astrocyte research protocols, enhanceing reproducibility and accelerating scientific progress in this critical area.

Most cited protocols related to «Astrocytes»

Purification and Culture of Human Brain Cells

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Publication 2015

Antibodies Antibodies, Anti-Idiotypic Astrocytes Brain Cell Culture Techniques Cells Endothelial Cells Fetus Gray Matter Homo sapiens Hybridomas Hyperostosis, Diffuse Idiopathic Skeletal Lectin Lysine Macrophage Microglia Neurons Oligodendrocyte Precursor Cells Oligodendroglia Papain Poly A Protease Inhibitors RNA-Seq Serum Thy-1 Antigens Tissues Trypsin

RNA-seq Transcriptome Profiling in Mice

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Publication 2015

Animals Astrocytes Calcium In Situ Hybridization Mice, House RNA-Seq

Modeling Alzheimer's Disease in iPSC-derived Neurons

Primary fibroblast cultures were established from dermal punch biopsies taken from individuals following informed consent and Institutional Review Board approval. To generate iPSCs, fibroblasts were transduced with MMLV vectors containing the complementary DNAs for OCT4, SOX2, KLF4, c-MYC and ± EGFP. IPSC-derived NPCs were differentiated for 3 weeks, neurons were purified by FACS, and amyloid-β, p-tau/total tau and aGSK-3β were measured on purified control and mutant neurons from multiple lines cultured in parallel for an additional 5 days by multi-spot electrochemiluminescence assays (Meso Scale Diagnostics). Early endosomes were analysed by confocal microscopy on purified neurons co-cultured with human astrocytes (Lonza) for 12 days. To ensure reproducible and consistent data, we found that it is important to differentiate and evaluate neurons from full sets of mutant and control iPSC lines together.

Israel M.A., Yuan S.H., Bardy C., Reyna S.M., Mu Y., Herrera C., Hefferan M.P., Van Gorp S., Nazor K.L., Boscolo F.S., Carson C.T., Laurent L.C., Marsala M., Gage F.H., Remes A.M., Koo E.H, & Goldstein L.S. (2012). Probing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells. Nature, 482(7384), 216-220.

Publication 2012

Amyloid Astrocytes Biological Assay Biopsy Cloning Vectors Diagnosis DNA, Complementary Endocytic Vesicles Ethics Committees, Research Fibroblasts Homo sapiens Induced Pluripotent Stem Cells KLF4 protein, human Microscopy, Confocal Neurons Oncogenes, myc POU5F1 protein, human SOX2 protein, human

Modeling Alzheimer's Disease in iPSC-derived Neurons

Publication 2012

Visualizing Perivascular Astrocytes in Mice

All experiments were approved by the University Committee on Animal Resources of the University of Rochester Medical Center. Unless otherwise noted, we used 8- to 12-week-old male C57BL/6 mice (Charles River). FVB/N-Tg(GFAPGFP)14Mes/J (GFAP-GFP, JAX) mice were used to visualize perivascular astrocytic endfeet. NG2-DsRed and Tie2-GFP:NG2-DsRed were used to identify arteries/arterioles versus veins/venules by endogenous fluorescence: Arteries and arterioles express endothelial GFP and vascular smooth muscle DsRed, and veins and venules express endothelial GFP but lack vascular smooth muscle DsRed. Aqp4^−/− (Aqp4-null) mice were generated as described (53 (link)).

Iliff J.J., Wang M., Liao Y., Plogg B.A., Peng W., Gundersen G.A., Benveniste H., Vates G.E., Deane R., Goldman S.A., Nagelhus E.A, & Nedergaard M. (2012). A Paravascular Pathway Facilitates CSF Flow Through the Brain Parenchyma and the Clearance of Interstitial Solutes, Including Amyloid β. Science translational medicine, 4(147), 147ra111.

Publication 2012

Animals Arteries Arterioles Astrocytes Endothelium Fluorescence Glial Fibrillary Acidic Protein Males Mice, Inbred C57BL Mice, Knockout Mice, Laboratory Muscle, Smooth, Vascular Rivers Veins Venules

Most recents protocols related to «Astrocytes»

Xenon Liposome Protects Astrocytes

Not available on PMC !

Example 5

Human brain astrocytes play a key role in maintaining nerve cell function and survival against oxidative stress. Exposure of cultured human brain astrocytes to H₂O₂causes significant damages to the cells, and caused them to release large amounts of LDH. However, pretreatment of the brain cells with Xe-ELIP reagents markedly reduced LDH release (FIG. 15), indicating a protective effect of Xe-ELIP on the brain cells injured by the oxidative stress. No or little protective effect was found in the cells treated with ELIP alone or control media (FIG. 15).

US11872312B2. Neuroprotective liposome compositions and methods for treatment of stroke (2024-01-16). The Board of Regents of The University of Texas System [US]. Inventors: Shao-Ling Huang [US], Melvin E. Klegerman [US], Yong-Jian Geng [US], Hyunggun Kim [US], David D. McPherson [US].

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Patent 2024

Astrocytes Brain Cells Cytotoxin Homo sapiens Neurons Oxidative Stress Peroxide, Hydrogen

Astrocyte Thermal Stability Assay

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Publication 2023

Astrocytes celastrol Centrifugation Nitrogen Cycle Protease Inhibitors Proteins Sulfoxide, Dimethyl

Hypoxia-Reoxygenation Neuronal and Astrocyte Assay

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Publication 2023

Aftercare Astrocytes celastrol Cells Culture Media Glucose Hypoxia Neurons Phosphates Saline Solution Sulfoxide, Dimethyl

Celastrol Target Identification in Astrocytes

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Publication 2023

Acetone Amines Astrocytes Azides Biotin celastrol Proteins Sepharose Sodium Ascorbate Tandem Mass Spectrometry Tromethamine tyrosyl-alanyl-glycine

Neuron-Astrocyte Co-Culture Protocol

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Publication 2023

Astrocytes Coculture Techniques Glucose Neurons

Top products related to «Astrocytes»

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific

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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

Penicillin streptomycin by Thermo Fisher Scientific

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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Streptomycin by Thermo Fisher Scientific

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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

Penicillin by Thermo Fisher Scientific

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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

Dmem f12 by Thermo Fisher Scientific

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DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.

Lipofectamine 2000 by Thermo Fisher Scientific

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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.

Glutamax by Thermo Fisher Scientific

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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.

Neurobasal medium by Thermo Fisher Scientific

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Neurobasal medium is a cell culture medium designed for the maintenance and growth of primary neuronal cells. It provides a defined, serum-free environment that supports the survival and differentiation of neurons. The medium is optimized to maintain the phenotypic characteristics of neurons and minimizes the growth of non-neuronal cells.

Astrocyte medium by ScienCell

Sourced in United States

Astrocyte medium is a specialized cell culture medium designed to support the growth and maintenance of astrocytes, a type of glial cell found in the central nervous system. The medium provides the necessary nutrients and growth factors to sustain astrocyte cultures, ensuring their optimal performance in various research applications.

What are the key functions of astrocytes in the central nervous system?

Astrocytes are a critical type of glial cell that perform a variety of essential functions in the central nervous system. They provide structural and metabolic support for neurons, regulate the blood-brain barrier, and modulate synaptic transmission. Astrocytes play a crucial role in maintaining homeostasis and neuronal health within the brain and spinal cord, making them a key focus for research into neurodegenerative diseases, brain injury, and other neurological conditions.

How can PubCompare.ai help optimize astrocyte research protocols?

PubCompare.ai is an AI-driven platform that can help researchers optimize their astrocyte research protocols. It allows you to efficiently screen protocol literature, leveraging advanced AI algorithms to pinpoint critical insights. The platform can help you identify the most effective protocols related to astrocytes for your specific research goals, highlighting key differences in protocol effectiveness so you can choose the best option for reproducibility and accruacy.

Are there different types or variations of astrocytes?

Yes, there are several different types and variations of astrocytes. For example, protoplasmic astrocytes are found in the grey matter of the brain, while fibrous astrocytes are predominant in the white matter. There are also specialized subtypes like radial glial cells and Bergmann glia that play unique roles. Understanding these astrocyte diversite is an active area of research, as different subtypes may be involved in distinct neurological processes and pathologies.

What are some common applications of astrocyte research?

Astrocytes have many important applications in neuroscience research and clinical settings. Studying astrcyte biology and function is key to advancing our understanding of neurodegenerative diseases like Alzheimer's, Parkinson's, and ALS, as well as brain injuries and neuroinflammatory conditions. Astrocytes also play a critical role in the blood-brain barrier, making them a target for research on drug delivery to the brain. Additionally, astrocyte transplantation is being explored as a potential therapy for spinal cord injury and other neurological traumas.

How can I use PubCompare.ai to optimize my astrocyte research protocols?

PubCompare.ai can help optimize your astrocyte research protocols in several ways. First, the platfrom allows you to efficiently screen the protocol literature, saving you time and effort. The AI-driven analyis can then help you identify the most effective protocols for your specific research goals, highlighting key differences that impact reproducibility and accuracy. This enables you to choose the best options to advance your astrocyte studies and accelerate scientific progress in this critical area of neuroscience reserach. Give PubCompare.ai a try to experience the power of AI-driven protocol optimization firsthand.

More about "Astrocytes"

Astrocytes are a crucial component of the central nervous system (CNS), playing vital roles in supporting and maintaining the health and function of neurons.
These star-shaped glial cells, found throughout the brain and spinal cord, perform a wide range of essential functions, including providing structural and metabolic support, regulating the blood-brain barrier, and modulating synaptic transmission.
Astrocytes are known to express various receptors and transporters, allowing them to respond to neurotransmitters and other signaling molecules.
They are also involved in the uptake and recycling of neurotransmitters, such as glutamate, helping to maintain the delicate balance required for proper neuronal communication.
In addition to their supportive roles, astrocytes are actively involved in the immune response within the CNS.
They can become reactive in response to injury, infection, or neurodegeneration, undergoing morphological and functional changes that contribute to the inflammatory process and tissue repair.
Understanding the complex biology and functions of astrocytes is crucial for advancing research into a wide range of neurological conditions, including neurodegenerative diseases (e.g., Alzheimer's, Parkinson's, and Huntington's diseases), brain injuries (e.g., traumatic brain injury and stroke), and neuropsychiatric disorders (e.g., schizophrenia and depression).
Researchers working with astrocytes often utilize specialized cell culture media and reagents, such as Fetal Bovine Serum (FBS), Dulbecco's Modified Eagle's Medium (DMEM), Penicillin/Streptomycin, DMEM/F12, Lipofectamine 2000, GlutaMAX, and Neurobasal medium, to maintain and differentiate these cells in vitro.
The PubCompare.ai platform offers a powerful AI-driven tool to help optimize research protocols, enhance reproducibility, and accelerate scientific progress in this critical area of neuroscience.