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Astrocytes

Astrocytes are a type of glial cell found in the central nervous system.
They perform a variety of essential functions, including providing structural and metabolic support for neurons, regulating the blood-brain barrier, and modulating synaptic transmission.
Astrocytes play a crucial role in maintaining homeostasis and neuronal health within the brain and spinal cord.
Understanding the biology and function of astrocstes is key to advancing research into neurodegenerative diseases, brain injury, and other neurological conditions.
The PubCompare.ai platform offers a powerful AI-driven tool to help researchers optimize their astrocyte research protocols, enhanceing reproducibility and accelerating scientific progress in this critical area.

Most cited protocols related to «Astrocytes»

1
Purification and Culture of Human Brain Cells

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Publication 2015
Antibodies Antibodies, Anti-Idiotypic Astrocytes Brain Cell Culture Techniques Cells Endothelial Cells Fetus Gray Matter Homo sapiens Hybridomas Hyperostosis, Diffuse Idiopathic Skeletal Lectin Lysine Macrophage Microglia Neurons Oligodendrocyte Precursor Cells Oligodendroglia Papain Poly A Protease Inhibitors RNA-Seq Serum Thy-1 Antigens Tissues Trypsin
2
RNA-seq Transcriptome Profiling in Mice

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Publication 2015
Animals Astrocytes Calcium In Situ Hybridization Mice, House RNA-Seq
3
Modeling Alzheimer's Disease in iPSC-derived Neurons
Primary fibroblast cultures were established from dermal punch biopsies taken from individuals following informed consent and Institutional Review Board approval. To generate iPSCs, fibroblasts were transduced with MMLV vectors containing the complementary DNAs for OCT4, SOX2, KLF4, c-MYC and ± EGFP. IPSC-derived NPCs were differentiated for 3 weeks, neurons were purified by FACS, and amyloid-β, p-tau/total tau and aGSK-3β were measured on purified control and mutant neurons from multiple lines cultured in parallel for an additional 5 days by multi-spot electrochemiluminescence assays (Meso Scale Diagnostics). Early endosomes were analysed by confocal microscopy on purified neurons co-cultured with human astrocytes (Lonza) for 12 days. To ensure reproducible and consistent data, we found that it is important to differentiate and evaluate neurons from full sets of mutant and control iPSC lines together.
Israel M.A., Yuan S.H., Bardy C., Reyna S.M., Mu Y., Herrera C., Hefferan M.P., Van Gorp S., Nazor K.L., Boscolo F.S., Carson C.T., Laurent L.C., Marsala M., Gage F.H., Remes A.M., Koo E.H, & Goldstein L.S. (2012). Probing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells. Nature, 482(7384), 216-220.
Publication 2012
Amyloid Astrocytes Biological Assay Biopsy Cloning Vectors Diagnosis DNA, Complementary Endocytic Vesicles Ethics Committees, Research Fibroblasts Homo sapiens Induced Pluripotent Stem Cells KLF4 protein, human Microscopy, Confocal Neurons Oncogenes, myc POU5F1 protein, human SOX2 protein, human
4
Modeling Alzheimer's Disease in iPSC-derived Neurons
Primary fibroblast cultures were established from dermal punch biopsies taken from individuals following informed consent and Institutional Review Board approval. To generate iPSCs, fibroblasts were transduced with MMLV vectors containing the complementary DNAs for OCT4, SOX2, KLF4, c-MYC and ± EGFP. IPSC-derived NPCs were differentiated for 3 weeks, neurons were purified by FACS, and amyloid-β, p-tau/total tau and aGSK-3β were measured on purified control and mutant neurons from multiple lines cultured in parallel for an additional 5 days by multi-spot electrochemiluminescence assays (Meso Scale Diagnostics). Early endosomes were analysed by confocal microscopy on purified neurons co-cultured with human astrocytes (Lonza) for 12 days. To ensure reproducible and consistent data, we found that it is important to differentiate and evaluate neurons from full sets of mutant and control iPSC lines together.
Israel M.A., Yuan S.H., Bardy C., Reyna S.M., Mu Y., Herrera C., Hefferan M.P., Van Gorp S., Nazor K.L., Boscolo F.S., Carson C.T., Laurent L.C., Marsala M., Gage F.H., Remes A.M., Koo E.H, & Goldstein L.S. (2012). Probing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells. Nature, 482(7384), 216-220.
Publication 2012
Amyloid Astrocytes Biological Assay Biopsy Cloning Vectors Diagnosis DNA, Complementary Endocytic Vesicles Ethics Committees, Research Fibroblasts Homo sapiens Induced Pluripotent Stem Cells KLF4 protein, human Microscopy, Confocal Neurons Oncogenes, myc POU5F1 protein, human SOX2 protein, human
5
Visualizing Perivascular Astrocytes in Mice
All experiments were approved by the University Committee on Animal Resources of the University of Rochester Medical Center. Unless otherwise noted, we used 8- to 12-week-old male C57BL/6 mice (Charles River). FVB/N-Tg(GFAPGFP)14Mes/J (GFAP-GFP, JAX) mice were used to visualize perivascular astrocytic endfeet. NG2-DsRed and Tie2-GFP:NG2-DsRed were used to identify arteries/arterioles versus veins/venules by endogenous fluorescence: Arteries and arterioles express endothelial GFP and vascular smooth muscle DsRed, and veins and venules express endothelial GFP but lack vascular smooth muscle DsRed. Aqp4−/− (Aqp4-null) mice were generated as described (53 (link)).
Iliff J.J., Wang M., Liao Y., Plogg B.A., Peng W., Gundersen G.A., Benveniste H., Vates G.E., Deane R., Goldman S.A., Nagelhus E.A, & Nedergaard M. (2012). A Paravascular Pathway Facilitates CSF Flow Through the Brain Parenchyma and the Clearance of Interstitial Solutes, Including Amyloid β. Science translational medicine, 4(147), 147ra111.
Publication 2012
Animals Arteries Arterioles Astrocytes Endothelium Fluorescence Glial Fibrillary Acidic Protein Males Mice, Inbred C57BL Mice, Knockout Mice, Laboratory Muscle, Smooth, Vascular Rivers Veins Venules

Most recents protocols related to «Astrocytes»

1
Xenon Liposome Protects Astrocytes
Not available on PMC !

Example 5

Human brain astrocytes play a key role in maintaining nerve cell function and survival against oxidative stress. Exposure of cultured human brain astrocytes to H2O2 causes significant damages to the cells, and caused them to release large amounts of LDH. However, pretreatment of the brain cells with Xe-ELIP reagents markedly reduced LDH release (FIG. 15), indicating a protective effect of Xe-ELIP on the brain cells injured by the oxidative stress. No or little protective effect was found in the cells treated with ELIP alone or control media (FIG. 15).

US11872312B2. Neuroprotective liposome compositions and methods for treatment of stroke (2024-01-16). The Board of Regents of The University of Texas System [US]. Inventors: Shao-Ling Huang [US], Melvin E. Klegerman [US], Yong-Jian Geng [US], Hyunggun Kim [US], David D. McPherson [US].
Patent 2024
Astrocytes Brain Cells Cytotoxin Homo sapiens Neurons Oxidative Stress Peroxide, Hydrogen
2
Astrocyte Thermal Stability Assay

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Publication 2023
Astrocytes celastrol Centrifugation Nitrogen Cycle Protease Inhibitors Proteins Sulfoxide, Dimethyl
3
Hypoxia-Reoxygenation Neuronal and Astrocyte Assay

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Publication 2023
Aftercare Astrocytes celastrol Cells Culture Media Glucose Hypoxia Neurons Phosphates Saline Solution Sulfoxide, Dimethyl
4
Celastrol Target Identification in Astrocytes

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Publication 2023
Acetone Amines Astrocytes Azides Biotin celastrol Proteins Sepharose Sodium Ascorbate Tandem Mass Spectrometry Tromethamine tyrosyl-alanyl-glycine
5
Neuron-Astrocyte Co-Culture Protocol

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Publication 2023
Astrocytes Coculture Techniques Glucose Neurons

Top products related to «Astrocytes»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
5
Penicillin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco
Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
6
Dmem f12 by Thermo Fisher Scientific
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DMEM/F12 is a cell culture medium developed by Thermo Fisher Scientific. It is a balanced salt solution that provides nutrients and growth factors essential for the cultivation of a variety of cell types, including adherent and suspension cells. The medium is formulated to support the proliferation and maintenance of cells in vitro.
7
Lipofectamine 2000 by Thermo Fisher Scientific
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
8
Glutamax by Thermo Fisher Scientific
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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
9
Neurobasal medium by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, Italy, Canada, Spain, France, Switzerland, China, Australia, Israel, Denmark, Ireland, Sweden, Austria
Neurobasal medium is a cell culture medium designed for the maintenance and growth of primary neuronal cells. It provides a defined, serum-free environment that supports the survival and differentiation of neurons. The medium is optimized to maintain the phenotypic characteristics of neurons and minimizes the growth of non-neuronal cells.
10
Astrocyte medium by ScienCell
Sourced in United States
Astrocyte medium is a specialized cell culture medium designed to support the growth and maintenance of astrocytes, a type of glial cell found in the central nervous system. The medium provides the necessary nutrients and growth factors to sustain astrocyte cultures, ensuring their optimal performance in various research applications.

More about "Astrocytes"

Astrocytes are a crucial component of the central nervous system (CNS), playing vital roles in supporting and maintaining the health and function of neurons.
These star-shaped glial cells, found throughout the brain and spinal cord, perform a wide range of essential functions, including providing structural and metabolic support, regulating the blood-brain barrier, and modulating synaptic transmission.
Astrocytes are known to express various receptors and transporters, allowing them to respond to neurotransmitters and other signaling molecules.
They are also involved in the uptake and recycling of neurotransmitters, such as glutamate, helping to maintain the delicate balance required for proper neuronal communication.
In addition to their supportive roles, astrocytes are actively involved in the immune response within the CNS.
They can become reactive in response to injury, infection, or neurodegeneration, undergoing morphological and functional changes that contribute to the inflammatory process and tissue repair.
Understanding the complex biology and functions of astrocytes is crucial for advancing research into a wide range of neurological conditions, including neurodegenerative diseases (e.g., Alzheimer's, Parkinson's, and Huntington's diseases), brain injuries (e.g., traumatic brain injury and stroke), and neuropsychiatric disorders (e.g., schizophrenia and depression).
Researchers working with astrocytes often utilize specialized cell culture media and reagents, such as Fetal Bovine Serum (FBS), Dulbecco's Modified Eagle's Medium (DMEM), Penicillin/Streptomycin, DMEM/F12, Lipofectamine 2000, GlutaMAX, and Neurobasal medium, to maintain and differentiate these cells in vitro.
The PubCompare.ai platform offers a powerful AI-driven tool to help optimize research protocols, enhance reproducibility, and accelerate scientific progress in this critical area of neuroscience.