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Bone Marrow Cells

Bone marrow cells are a diverse population of cells found within the bone marrow, which serves as the primary site of hematopoiesis in adults.
These cells include hematopoietic stem cells, progenitor cells, and a variety of mature blood cell types such as erythrocytes, leukocytes, and platelets.
Bone marrow cells play a crucial role in the maintenance and replenishment of the body's blood and immune systems.
They are of great interest in the field of regenerative medicine due to their potential for differentiation into various cell types, including osteoblasts, adipocytes, and chondrocytes.
Researchers utilize bone marrow cells in a variety of applications, such as cell therapies, tissue engineering, and the study of hematological disorders.
PubCompare.ai's AI-driven platform can help streamline research on bone marrow cells by locating the best protocols from literature, preprints, and patents, and identifying the most effective approaches and products.

Most cited protocols related to «Bone Marrow Cells»

Mass Cytometry Data Analysis Workflow

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Publication 2015

Bone Marrow Cells

Validating CD69+ T-cell Population by Bulk RNA-seq

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Publication 2019

Bone Marrow Cells Buffers CD4 Positive T Lymphocytes CD8-Positive T-Lymphocytes Cells Clone Cells DAPI Dietary Fiber Division, Cell DNA, Complementary Edetic Acid Fluorescein-5-isothiocyanate Genes Immunoglobulins Love Population Group Reverse Transcription RNA-Seq

Bone Marrow-Derived Macrophage Generation

Fresh or frozen bone marrow cells were used to generate BMDM as previously described [8] (link), using L929-cell conditioned medium (LCCM) as a source of granulocyte/macrophage colony stimulating factor [9] (link). The cells were resuspended in 10 ml bone marrow differentiation media (R20/30), which is RPMI1640 supplemented with 20% fetal bovine serum (Gibco, cat. 12657-029), 30% LCCM, 100 U/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. Cells were seeded in non-tissue culture treated Optilux Petri dishes (BD Biosciences) and incubated at 37°C in a 5% CO₂ atmosphere. Four days after seeding the cells, an extra 10 ml of fresh R20/30 were added per plate and incubated for an additional 3 days. To obtain the BMDM, the supernatants were discarded and the attached cells were washed with 10 ml of sterile PBS. Ten ml of ice-cold PBS were added to each plate and incubated at 4°C for 10 minutes. The macrophages were detached by gently pipetting the PBS across the dish. The cells were centrifuged at 200× g for 5 minutes and resuspended in 10 ml of BMDM cultivation media (R10/5), which is composed of RPMI 1640, 10% fetal bovine serum, 5% LCCM and 2 mM L-glutamine. The cells were counted, seeded and cultivated in tissue culture plates 12 hours before any further experimental procedure.

Marim F.M., Silveira T.N., Lima DS J.r, & Zamboni D.S. (2010). A Method for Generation of Bone Marrow-Derived Macrophages from Cryopreserved Mouse Bone Marrow Cells. PLoS ONE, 5(12), e15263.

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Publication 2010

Atmosphere Bone Marrow Bone Marrow Cells Cells Common Cold Culture Media, Conditioned Fetal Bovine Serum Freezing Glutamine Granulocyte-Macrophage Colony-Stimulating Factor Hyperostosis, Diffuse Idiopathic Skeletal L929 Cells Macrophage Penicillins Sterility, Reproductive Streptomycin Tissues

Differentiation of Murine Bone Marrow Cells

Bone marrow cells were collected from the femurs and tibiae of wild-type BALB/c mice (Taconic) by flushing the opened bones with Iscove's Modified Dulbecco's Medium (IMDM; Invitrogen). Red blood cells were lysed in dH₂O followed by the addition of 10X PBS. After centrifugation, the cells were washed once in PBS containing 0.1% BSA. The bone marrow cells were cultured at 10⁶/mL in media containing RPMI 1640 (Invitrogen) with 20% FBS (Cambrex), 100 IU/mL penicillin and 10 μg/mL streptomycin (Cellgro), 2 mM glutamine (Invitrogen), 25 mM HEPES and 1x non-essential amino acids and 1 mM sodium pyruvate (Gibco) and 50 μM β-mercaptoethanol (Sigma) and supplemented with 100 ng/mL stem-cell factor (SCF; PeproTech) and 100 ng/mL FLT3-Ligand (FLT3-L; PeproTech) from day 0 to day 4. On day 4, the media containing SCF and FLT3-L was replaced with media containing 10 ng/mL recombinant mouse interleukin-5 (rmIL-5; R&D Systems) only. On day 8, the cells were moved to new flasks and maintained in fresh media supplemented with rmIL-5. Every other day, from this point forward, one-half of the media was replaced with fresh media containing rmIL-5, and the concentration of the cells was adjusted each time to10⁶ /mL. Cells were enumerated at day 0 and on days indicated thereafter in a hemocytometer, and 50,000 cells were subjected to cytospin (Thermo Shandon, Pittsburgh, PA). The cytospin preparations were fixed and stained using a modified Giemsa preparation (Diff Quik, Dade Behring AG, Dudingen, Switzerland).

Dyer K.D., Moser J.M., Czapiga M., Siegel S.J., Percopo C.M, & Rosenberg H.F. (2008). Functionally competent eosinophils differentiated ex vivo in high purity from normal mouse bone marrow. Journal of immunology (Baltimore, Md. : 1950), 181(6), 4004-4009.

Publication 2008

2-Mercaptoethanol 3,3'-diallyldiethylstilbestrol Amino Acids, Essential Bone Marrow Cells Bones Cells Centrifugation Diff Quik Erythrocytes Femur flt3 ligand FLT3 protein, human Glutamine HEPES Interleukin-5 Mice, Inbred BALB C Mus Penicillins Pyruvate Sodium Stain, Giemsa Stem Cell Factor Streptomycin Tibia

Immunological Genome Project Data Analysis

Immunological Genome Project (ImmGen) raw expression data of phase 1 and 2 were downloaded as CEL files from GEO (GSE15907 and GSE37448), processed and normalized using the Robust Multi-array Average (RMA) procedure on probe-level data using Matlab functions. The analysis was performed using custom CDF file obtained from Brainarray^{50 (link)}. Blueprint and Encode data were processed as we described previously^{51 (link)}. Raw counts from the lung reference dataset were obtained from Gibbings et al.^{27 (link)} (GSE94135) and Altboum et al.^{26 (link)} (GSE49932). Counts were normalized and converted to TPM using R functions. Gene expression profile of GM-CSF derived bone marrow dendritic cell subsets (available at GSE62361¹⁸) was analyzed using the GEO2R tool for differentially expressed genes. Top 50 upregulated by fold-change of GM-DCs genes and top 50 GM-Macs were used in Figure 1b. Single-cell RNA-seq counts from Hashimshony et al.^{15 (link)} used in Figure 1b were downloaded from the ARCHS project^{52 (link)}. 10X PBMC single-cell counts were downloaded directly from the 10X website (https://support.10xgenomics.com/single-cell-gene-expression/datasets ).

Aran D., Looney A.P., Liu L., Wu E., Fong V., Hsu A., Chak S., Naikawadi R.P., Wolters P.J., Abate A.R., Butte A.J, & Bhattacharya M. (2019). Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nature immunology, 20(2), 163-172.

Publication 2018

Bone Marrow Cells Cells Dendrites Gene Expression Genes Genome Granulocyte-Macrophage Colony-Stimulating Factor Lung RNA-Seq

Most recents protocols related to «Bone Marrow Cells»

Osteoclast Differentiation from Murine Bone Marrow

Bone marrow cells extracted from femurs and tibiae of 10–12-wk old mice were cultured in α-MEM containing 10% FBS, 100 IU/ml penicillin, and 100 μg/ml streptomycin with 20 ng/ml recombinant mouse M-CSF (416-ML; R&D Systems) in plastic petri dishes. Cells were incubated at 37°C in 95% air/5% CO₂ for 4 d and then lifted with 5 mM EDTA in PBS. Recovered BMDMs were cultured in α-MEM containing 10% FBS supplemented with 20 ng/ml mouse M-CSF and 30 ng/ml mouse RANKL (462-TEC; R&D Systems) for 5 d in tissue-culture dishes to induce osteoclast formation (Tang et al., 2009 (link); Zhu et al., 2020 (link)). Mature osteoclasts were characterized by staining for TRAP activity using an Acid Phosphatase Leukocyte Kit (387A; Sigma-Aldrich) and TRAP-positive MNCs (>3 nuclei/cell) counted.

Zhu L., Tang Y., Li X.Y., Kerk S.A., Lyssiotis C.A., Sun X., Wang Z., Cho J.S., Ma J, & Weiss S.J. (2023). Proteolytic regulation of a galectin-3/Lrp1 axis controls osteoclast-mediated bone resorption. The Journal of Cell Biology, 222(4), e202206121.

Publication 2023

Acid Phosphatase Bone Marrow Cells Cell Nucleus Cells Edetic Acid Femur Hyperostosis, Diffuse Idiopathic Skeletal Leukocytes Macrophage Colony-Stimulating Factor Mus Osteoclasts Penicillins Streptomycin Tibia Tissues TNFSF11 protein, human

Mouse Bone Marrow-Derived Macrophage Isolation

Bone marrow cells were isolated by flushing the femur and tibia of 8-week-old male C57BL/6 mice. The isolated bone marrow cells were incubated with macrophage colony-stimulating factor (M-CSF; Peprotech, Cranbury, NJ, USA), and were differentiated (7 days) into BMDMs. Bone marrow cells were incubated in RPMI medium containing 30 ng/ml M-CSF, 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, and 25 mM HEPES. The media were changed every 3 days.

Kim H.Y., Otgontenger U., Kim J.W., Lee Y.J., Kim S.B., Lim S.C., Kim Y.M, & Kang K.W. (2023). Anti-fibrotic effect of aurocyanide, the active metabolite of auranofin. Archives of Pharmacal Research, 46(3), 149-159.

Publication 2023

Bone Marrow Cells Femur Fetal Bovine Serum HEPES Macrophage Colony-Stimulating Factor Males Mice, Inbred C57BL Penicillins Streptomycin Tibia

Sample Preparation Timing Across Cell Types

The time required to perform sample preparation
from the intact organ or cell culture all the way to the cell extract
depends on the number of replicates that are prepared in parallel,
the experience of the person performing the experiment, and the sample
preparation protocol at hand. We estimate the times that were required
for preparing the samples for this work as follows: peripheral blood
cells: 60 min, stem cells and progenitor cells from bone marrow: 5
hours, tissue-resident immune cells from skin: 4 hours, and splenocyte
and cultured Jurkat cells: 30 min.

Schönberger K., Mitterer M., Glaser K., Stecher M., Hobitz S., Schain-Zota D., Schuldes K., Lämmermann T., Rambold A.S., Cabezas-Wallscheid N, & Buescher J.M. (2023). LC-MS-Based Targeted Metabolomics for FACS-Purified Rare Cells. Analytical Chemistry, 95(9), 4325-4334.

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Publication 2023

Bone Marrow Cells Cell Culture Techniques Cells Cultured Cells Skin Stem Cells

Activation of Aged Murine BMDCs

The effects of combinations of polymeric nanoparticles, micelles, and various small molecule agonists on the activation of bone marrow derived DCs (BMDCs) harvested from the femur and tibia of aged (≥20 months) C57BL/6 male mice (n = 6) were studied. Bone marrow cells were cultured as outlined in Lutz et al. [101 (link)]. Briefly, cells were cultured in 10 mL RPMI 1640 medium supplemented with 100 U/mL penicillin, 100 mg/mL streptomycin, 2 mM glutamine, 10% FBS, and 20 ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF, Cat. #FB0875711Z, Peprotech, Rocky Hill, NJ) at approximately 5 × 10⁶ cells per 100 mm plate. On day 3 of culture, 10 mL of medium containing GM-CSF was added. On days 6 and 8 of the culture period, approximately half of the total volume of medium was removed and replaced with freshly supplemented RPMI. On day 10 plates were gently rinsed to harvest non-adherent DCs for assessment of activation and costimulatory expression. Animals were obtained from Jackson Laboratory (Bar Harbor, ME) and maintained at Iowa State University following IACUC protocol #IACUC-20-199.

Ananya A., Holden K.G., Gu Z., Nettleton D., Mallapragada S.K., Wannemuehler M.J., Kohut M.L, & Narasimhan B. (2023). “Just right” combinations of adjuvants with nanoscale carriers activate aged dendritic cells without overt inflammation. Immunity & Ageing : I & A, 20, 10.

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Publication 2023

agonists Animals Bone Marrow Bone Marrow Cells Cells Femur Glutamine Granulocyte-Macrophage Colony-Stimulating Factor Institutional Animal Care and Use Committees Males Mice, Inbred C57BL Micelles Penicillins Polymers Streptomycin Tibia

Assessing Tumor Burden in Leukemia Xenograft

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Publication 2023

Animals, Laboratory Bone Marrow Bone Marrow Cells Culture Media Ethics Committees, Clinical Femur Flow Cytometry HL-60 Cells Mice, Inbred NOD Mus SCID Mice Tail Transplantation Tumor Burden Veins Whole-Body Irradiation WISP2 protein, human Woman

Top products related to «Bone Marrow Cells»

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Gm csf by Thermo Fisher Scientific

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GM-CSF is a laboratory reagent used for cell culture applications. It is a recombinant human granulocyte-macrophage colony-stimulating factor that promotes the growth and differentiation of hematopoietic cells.

Penicillin streptomycin by Thermo Fisher Scientific

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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

M csf by Thermo Fisher Scientific

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M-CSF is a recombinant human macrophage colony-stimulating factor (M-CSF) that promotes the proliferation and differentiation of macrophages from hematopoietic progenitor cells. It functions as a cytokine and is involved in the regulation of macrophage production and function.

Penicillin by Thermo Fisher Scientific

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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

Streptomycin by Thermo Fisher Scientific

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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific

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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

Rpmi 1640 by Thermo Fisher Scientific

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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.

Lipopolysaccharides (lps) by Merck Group

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The LPS laboratory equipment is a high-precision device used for various applications in scientific research and laboratory settings. It is designed to accurately measure and monitor specific parameters essential for various experimental procedures. The core function of the LPS is to provide reliable and consistent data collection, ensuring the integrity of research results. No further details or interpretations can be provided while maintaining an unbiased and factual approach.

L glutamine by Thermo Fisher Scientific

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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.

What are bone marrow cells?

Bone marrow cells are a diverse population of cells found within the bone marrow, which serves as the primary site of hematopoiesis (blood cell production) in adults. These cells include hematopoietic stem cells, progenitor cells, and a variety of mature blood cell types such as erythrocytes (red blood cells), leukocytes (white blood cells), and platelets. Bone marrow cells play a crucial role in the maintenance and replenishment of the body's blood and immune systems.

What are the potential applications of bone marrow cells?

Bone marrow cells are of great interest in the field of regenerative medicine due to their potential for differentiation into various cell types, including osteoblasts (bone cells), adipocytes (fat cells), and chondrocytes (cartilage cells). Researchers utilize bone marrow cells in a variety of applications, such as cell therapies, tissue engineering, and the study of hematological (blood) disorders.

How can PubCompare.ai help with bone marrow cell research?

PubCompare.ai's AI-driven platform can help streamline your research on bone marrow cells. The platform allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. By identifying the most effective protocols related to bone marrow cells for your specific research goals, PubCompare.ai can help you choose the best option for reproducibility and accuracy, ultimately enhancing the quality of your research.

What are some common challenges in working with bone marrow cells?

One of the main challenges in working with bone marrow cells is the inherent diversity of the cell population. Bone marrow contains a mix of different cell types, each with their own unique properties and functions. Effectively isolating and purifying the desired cell type, such as hematopoietic stem cells or progenitor cells, can be a technical hurtle. Additionally, maintaining the viability and differentiation potential of bone marrow cells during in vitro culture and expansion can be a delicate process that requires careful optimization.

More about "Bone Marrow Cells"

Bone marrow cells, also known as hematopoietic cells or myeloid cells, are a diverse population of cells found within the bone marrow, which serves as the primary site of blood cell formation (hematopoiesis) in adults.
These cells include hematopoietic stem cells, progenitor cells, and a variety of mature blood cell types such as erythrocytes (red blood cells), leukocytes (white blood cells), and platelets.
Bone marrow cells play a crucial role in the maintenance and replenishment of the body's blood and immune systems.
They are of great interest in the field of regenerative medicine due to their potential for differentiation into various cell types, including osteoblasts (bone cells), adipocytes (fat cells), and chondrocytes (cartilage cells).
Researchers utilize bone marrow cells in a variety of applications, such as cell therapies, tissue engineering, and the study of hematological disorders.
Common cell culture media and supplements used in bone marrow cell research include Fetal Bovine Serum (FBS), Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Penicillin/Streptomycin, Macrophage Colony-Stimulating Factor (M-CSF), Penicillin, Streptomycin, Dulbecco's Modified Eagle Medium (DMEM), RPMI 1640, Lipopolysaccharide (LPS), and L-glutamine.
PubCompare.ai's AI-driven platform can help streamline research on bone marrow cells by locating the best protocols from literature, preprints, and patents, and identifying the most effective approaches and products.
This can help researchers optimize their studies and improve the reproducibility of their findings.