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Bone Marrow Mesenchymal Stem Cells

Mesenchymal stem cells derived from bone marrow.
These multipotent progenitor cells can differentiate into various cell types including osteoblasts, chondrocytes, myocytes, and adipocytes.
Bone marrow mesenchymal stem cells have potential applications in regenerative medicine and tissue engineering.

Most cited protocols related to «Bone Marrow Mesenchymal Stem Cells»

1
Chondrogenic Differentiation of Mesenchymal Stem Cells
Bone marrow-derived mesenchymal stem cells can be induced to differentiate into chondrocytes under specific culture conditions. These conditions include three-dimensional conformation of the cells in aggregates where high cell density and cell–cell interaction play an important role in the mechanism of chondrogenesis. Together with these physical culture conditions, a defined culture medium containing TGF-β1 is required to achieve chondrogenic differentiation (18 (link), 19 (link), 78 (link)). The culture conditions described below were initially developed for a small-scale chondrogenic differentiation assay, although we have also found that they work well for larger-scale bioreactor-based tissue engineering. Traditionally, these assays were performed in 15-ml polypropylene centrifuge tubes, but we have developed an improved method for preparing cell aggregates for in vitro chondrogenesis studies (79 (link)). This method replaces the original 15-ml polypropylene tubes with 96-well plates (see Fig. 8). These modifications allow a high-throughput approach to chondrogenic cultures, which reduces both the cost and time with no detrimental effects on the histological and histochemical qualities of the aggregates.

Centrifuge harvested cells (see Subheading 3.2 steps 1–11). in a benchtop centrifuge at 500 × g for 5 min.

Resuspend cells at a density of 1.25 × 106 cells/ml in chondrogenic differentiation medium.

Using a repeater pipette, dispense 0.2-ml aliquots of the cell suspension (2.5 × 105 cells/well) into polypropylene 96-well plates.

Spin aliquots in a benchtop centrifuge at 500 × g for 5 min.

Place the multiwell plates in the incubator at 37°C in a humidified atmosphere of 95% air and 5% CO2 for up to 3 weeks.

Twenty four hours after centrifugation, ensure that the aggregates can float freely by releasing them from the bottom of the wells by aspirating 100 μl of media and gently releasing it back into the wells using an eight-channel pipette.

Change chondrogenic medium every other day. Carefully aspirate expired medium using a sterile 200-μl pipette tip affixed to a vacuum system.

Aliquot 0.2 ml of fresh chondrogenic medium to each well.

Solchaga L.A., Penick K.J, & Welter J.F. (2011). Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks. Methods in molecular biology (Clifton, N.J.), 698, 253-278.
Publication 2011
Atmosphere Biological Assay Bioreactors Bone Marrow Mesenchymal Stem Cells Cell Communication Cells Centrifugation Chondrocyte Chondrogenesis Physical Examination Polypropylenes Sterility, Reproductive TGF-beta1 Vacuum
2
Isolation and Culture of Mouse Bone Marrow-Derived Macrophages and Osteoclasts
We cultured primary bone marrow cells isolated from C57/BL6 mice as described40 (link)41 (link). Briefly, Bone marrow cells were isolated from 8-week-old C57/BL6 mice by flushing femurs and tibias with α-MEM and cultured in α-MEM with 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin and M-CSF (30 ng/ml) overnight. Non-adherent cells were collected and further cultured in the presence of M-CSF (30 ng/ml) for 3 days. Floating cells were discarded and adherent cells were used as bone marrow-derived macrophages (BMMs). Preparation of mouse osteoclasts was carried out as described previously42 (link)43 (link). In brief, BMMs (4 × 104 cells/well) were seeded on a 0.2% collagen-gel coated 12-well plate and induced by RANKL (100 ng/mL) and M-CSF (30 ng/mL) for 6 days. Then osteoclasts were recovered by treatment with 0.2% collagenase, suspended in a-MEM containing 10% FBS, and used for osteoclast function assays.
Rat bone marrow mesenchymal stem cells (BMSCs) were isolated from 4-week-old Sprague–Dawley rats (male or female 80–100 g) and expanded in accordance with published techniques44 (link)45 (link)46 (link). The cells were maintained in expansion medium consisting of Dulbecco’s modified Eagle’s medium (DMEM)/F12, 10% FBS.
Zhang Y., Guan H., Li J., Fang Z., Chen W, & Li F. (2015). Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss. Scientific Reports, 5, 13575.
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Publication 2015
Biological Assay Bone Marrow Cells Bone Marrow Mesenchymal Stem Cells Cells Collagen Collagenase Eagle Females Femur Macrophage Macrophage Colony-Stimulating Factor Males Mus Osteoclasts Penicillins Rats, Sprague-Dawley Streptomycin Tibia TNFSF11 protein, human
3
Chondrogenic Differentiation of hBMSCs
3D pellets were formed using 2.5×105 human bone marrow-derived mesenchymal stem cells (hBMSCs). Chondrogenesis was initiated in serum-free chondrogenic differentiation medium. From day 14 onwards, cell pellets were maintained either in the standard chondrogenic differentiation medium or in the presence of T3 (10 nM) or IOP (10 μM). For additional details, see online supplemental methods.
Bomer N., den Hollander W., Ramos Y.F., Bos S.D., van der Breggen R., Lakenberg N., Pepers B.A., van Eeden A.E., Darvishan A., Tobi E.W., Duijnisveld B.J., van den Akker E.B., Heijmans B.T., van Roon-Mom W.M., Verbeek F.J., van Osch G.J., Nelissen R.G., Slagboom P.E, & Meulenbelt I. (2014). Underlying molecular mechanisms of DIO2 susceptibility in symptomatic osteoarthritis. Annals of the Rheumatic Diseases, 74(8), 1571-1579.
Publication 2014
Bone Marrow Mesenchymal Stem Cells Cells Chondrogenesis Homo sapiens Pellets, Drug Serum
4
Isolation of Human Bone Marrow-Derived Mesenchymal Stem Cells
Human bone marrow-mesenchymal stem cells (hBM-MSCs) were isolated as described by our group [28 (link),36 (link)]. hBM-MSCs were isolated from waste samples (washouts of the medullary cavities of patients’ femurs) from surgery to which adult donor subjects were subjected. The procedure was occasional and is not part of a specific project. All procedures were done with the consent of donors and in accordance with the Declaration of Helsinki. hBM-MSCs were isolated from the mononuclear cells of the density gradient (Lympholyte; Cedarlane Laboratories Limited, Hornby, ON, Canada) component of the bone marrow, by seeding in culture flasks in growth culture medium: Dulbecco’s Modified Eagle Medium (DMEM) high glucose (Euroclone S.p.A, Pero (MI), Italy) medium containing FBS 10%, 2 mM L-glutamine, and 1% penicillin–streptomycin (Euroclone S.p.A, Pero (MI), Italy) in a humidified atmosphere and 5% CO2 at 37 °C. Non-adherent cells were removed after 5 to 7 days, and the fresh medium was added to the flasks. After 15 days, a fibroblast-like colony started to grow. Isolated were seeded in TCP culture flasks and kept in culture using containing 10% FBS, 1% 2 mM L-glutamine, 1% penicillin/streptomycin (Euroclone S.p.A, Pero (MI), Italy) in humidified atmosphere at 37 °C, 5% CO2. Medium change occurred every three days.
Luzi F., Tortorella I., Di Michele A., Dominici F., Argentati C., Morena F., Torre L., Puglia D, & Martino S. (2020). Novel Nanocomposite PLA Films with Lignin/Zinc Oxide Hybrids: Design, Characterization, Interaction with Mesenchymal Stem Cells. Nanomaterials, 10(11), 2176.
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Publication 2020
Adult Atmosphere Bone Marrow Bone Marrow Mesenchymal Stem Cells Cells Dental Caries Donors Eagle Femur Fibroblasts Glucose Glutamine Homo sapiens Medulla Oblongata Operative Surgical Procedures Patients Penicillins Streptomycin Tissue Donors
5
Pluripotent Stem Cell Protocol

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Publication 2014
Bone Marrow Mesenchymal Stem Cells Flow Cytometry Human Embryonic Stem Cells Human Induced Pluripotent Stem Cells Immunofluorescence Myocytes, Cardiac

Most recents protocols related to «Bone Marrow Mesenchymal Stem Cells»

1
Seeding Mesenchymal Stem Cells in 3D Inserts
Not available on PMC !

Example 4

Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length by 10 mm height) were seeded with 5,000, or 10,000, or 20,000 P6 human bone marrow-derived mesenchymal stem cells per channel in 200 μl of culture media in a tissue culture dish on a rocking platform. Rocking frequency was 5 rpm and 15° tilt. FIGS. 6A-6F show cell aggregates with different cell seeding density at 12 and 24 hours of cell culture.

US11873472B2. Three-dimensional culture device and methods for dynamic culture of cell aggregates (2024-01-16). FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC. [US], None [US]. Inventors: Teng Ma [US], Ang-Chen Tsai [US], Xuegang Yuan [US].
Full text: Click here
Patent 2024
Bone Marrow Mesenchymal Stem Cells Cell Culture Techniques Cells Culture Media Figs Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Medical Devices Tissues
2
Mesenchymal Stem Cell Seeding in 3D Inserts
Not available on PMC !

Example 2

Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length or 25 mm length by 10 mm height) or square 3-D inserts (having three micro-channels of 15 mm length) were seeded with 50,000 or 100,000 P6 human bone marrow-derived mesenchymal stem cells per channel in 200 μl of culture media in a tissue culture dish on a rocking platform. Rocking frequency was 5 rpm and 15° tilt. Cells were cultured for two days. FIGS. 4A-4D show cell aggregates at 24 and 48 hours.

US11873472B2. Three-dimensional culture device and methods for dynamic culture of cell aggregates (2024-01-16). FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC. [US], None [US]. Inventors: Teng Ma [US], Ang-Chen Tsai [US], Xuegang Yuan [US].
Full text: Click here
Patent 2024
Bone Marrow Mesenchymal Stem Cells Cell Culture Techniques Cells Culture Media Figs Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Medical Devices Tissues
3
Stem Cell Aggregation in 3D Inserts
Not available on PMC !

Example 5

Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length by 10 mm height) were seeded with 10,000 P6 human bone marrow-derived mesenchymal stem cells per channel in 200 μl of culture media in a tissue culture dish on a rocking platform. Rocking frequency was 5 rpm and 15° tilt. FIGS. 7A-7C show cell aggregates dynamics with different time points of 6, 12 and 24 hours of cell culture.

US11873472B2. Three-dimensional culture device and methods for dynamic culture of cell aggregates (2024-01-16). FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC. [US], None [US]. Inventors: Teng Ma [US], Ang-Chen Tsai [US], Xuegang Yuan [US].
Full text: Click here
Patent 2024
Bone Marrow Mesenchymal Stem Cells Cell Culture Techniques Cells Culture Media Figs Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Medical Devices Tissues
4
3D Culture of Mesenchymal Stem Cells
Not available on PMC !

Example 1

A 3-D insert built in a 100 mm diameter non-adherent culture dish (Corning, Corning, NY) was used to culture human bone marrow-derived mesenchymal stem cells. Dimensions of the micro-channels were 3 mm width by 40 mm length by 10 mm height. 50,000 cells were seeded in each microchannel. Final volume of culture medium was adjusted to 400 uL. The culture dish was placed on a programmed rocking platform and cultured for 3 days with the setting at 5 rpm and 20° tilt. FIGS. 3A-3C shows cell aggregates at 24 hours, 48 hours, and 72 hours of culture. Aggregates were collected and replated on standard tissue culture plates for further culture (see FIGS. 3D and 3E).

US11873472B2. Three-dimensional culture device and methods for dynamic culture of cell aggregates (2024-01-16). FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC. [US], None [US]. Inventors: Teng Ma [US], Ang-Chen Tsai [US], Xuegang Yuan [US].
Full text: Click here
Patent 2024
Bone Marrow Mesenchymal Stem Cells Cell Culture Techniques Cells Culture Media Figs Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Medical Devices Tissues
5
Bone Marrow Stem Cell Culture
Not available on PMC !

Example 3

Round 3-D inserts (having three micro-channels of 3 mm width by 20 mm length by 10 mm height) were seeded with 10,000 or 20,000 P7 human bone marrow-derived mesenchymal stem cells per channel in a tissue culture dish on a rocking platform. Final media volume was adjusted to 200 uL. Rocking frequency was 5 rpm and 15° tilt. FIGS. 5A-5D show the cell aggregates generated at 24 and 48 hours of culture.

US11873472B2. Three-dimensional culture device and methods for dynamic culture of cell aggregates (2024-01-16). FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC. [US], None [US]. Inventors: Teng Ma [US], Ang-Chen Tsai [US], Xuegang Yuan [US].
Full text: Click here
Patent 2024
Bone Marrow Mesenchymal Stem Cells Cell Culture Techniques Cells Figs Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Medical Devices Tissues

Top products related to «Bone Marrow Mesenchymal Stem Cells»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
3
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
4
α mem by Thermo Fisher Scientific
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α-MEM is a cell culture medium formulated for the growth and maintenance of mammalian cells. It provides a balanced salt solution, amino acids, vitamins, and other nutrients required for cell proliferation.
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Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
6
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Dexamethasone (dex) by Merck Group
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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
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L glutamine by Thermo Fisher Scientific
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
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β glycerophosphate by Merck Group
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β-glycerophosphate is a chemical compound that serves as a buffering agent and source of phosphate for cell culture media. It helps maintain a stable pH environment for cell growth and proliferation.
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Ascorbic acid by Merck Group
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Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.

More about "Bone Marrow Mesenchymal Stem Cells"

Bone Marrow Mesenchymal Stem Cells (BM-MSCs) are a type of multipotent progenitor cells derived from the bone marrow.
These cells have the ability to differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells), and adipocytes (fat cells).
BM-MSCs have garnered significant interest in the field of regenerative medicine and tissue engineering due to their remarkable regenerative potential.
Culturing and maintaining BM-MSCs typically involves the use of various media supplements, such as Fetal Bovine Serum (FBS), Penicillin/Streptomycin, Dulbecco's Modified Eagle Medium (DMEM), and α-Minimum Essential Medium (α-MEM).
These components provide the necessary nutrients, growth factors, and antibiotics to support the growth and differentiation of BM-MSCs in vitro.
In addition to the culture media, other key substances used in BM-MSC research and applications include Penicillin, Streptomycin (antibiotics), Dexamethasone (a synthetic glucocorticoid), L-glutamine (an essential amino acid), β-glycerophosphate (a source of phosphate), and Ascorbic acid (vitamin C).
These compounds play crucial roles in various aspects of BM-MSC biology, such as proliferation, differentiation, and extracellular matrix formation.
By leveraging the insights provided by PubCompare.ai's AI-powered platform, researchers can optimize their protocols for working with Bone Marrow Mesenchymal Stem Cells.
The platform enables the identification of the best protocols from literature, preprints, and patents, and provides AI-driven comparisons to enhance reproducibility and drive research forward in this exciting field of regenerative medicine.