Log In Sign up
The largest database of trusted experimental protocols
> Anatomy > Cell > Chief Cells, Gastric

Chief Cells, Gastric

Chief cells are the predominant secretory cell type found in the gastric glands of the stomach.
These cells produce and secrete pepsinogen, an inactive enzyme precursor that is converted to the active digestive enzyme pepsin upon exposure to the acidic environment of the stomach.
Chief cells play a crucial role in the process of gastric digestion, contributing to the breakdown of proteins.
Understanding the function and regulation of chief cells is important for research related to gastrointestinal physiology, acid-base balance, and digestive disorders.
PubCompare.ai's platform can help identify the most accurate and reliable protocols for studying chief cells and gastric function from the scientific literatrue, pre-prints, and patents.

Most cited protocols related to «Chief Cells, Gastric»

1
Isolation and Characterization of Intestinal and Splenic Dendritic Cells
For LpDCs, total Lp cells after digestion with Liberase CI were passed through 70- and 40-μm cell strainers. Cells were resuspended in 1.077 g/cm3 iso-osmotic NycoPrep medium (Accurate Chemical & Scientific Corp.), and the low-density fraction was collected after centrifugation at 1,650 g for 15 min. Nycodenz gradient excludes debris and red blood cells and decreases lymphocyte numbers without changing the composition of the different subsets of LpDC (Fig. S5, available at http://www.jem.org/cgi/content/full/jem.20070663/DC1). Cells were washed and incubated with a mixture of mAb containing α−CD11c (HL-3), α−MHC II (AF6-120.1), α−CD16/32 (2.4G2), and α−CD103 mAb (2E7), as well as the non-DC components α−DX5 (DX5), α-NK1.1 (PK136), and α−B220 (RA3-6B2, all from eBioscience). DCs were defined as CD11c+MHCII+ cells and non-DCs were excluded when sorted by flow cytometry on a FACSVantage or FACSAria. In some experiments, CD103+ and CD103− DCs were separated. Purity was verified by flow cytometry on a FACSCalibur. I-Ab+CD11c+ cells were >90%.
For SpDCs, spleens were cut into fragments and digested by 100 mg/ml Liberase CI and 150 mg/ml DNase I, and then dissociated in Ca2+-free medium in the presence of EDTA. IAb+CD11c+ SpDCs were further purified for LpDCs, resulting in >98% purity. For phenotypic analysis, I-Ab+CD11c+ DCs were stained with fluorescent dye-conjugated α-CD11b (M1/70), α-CD103 (2E7; all from eBioscience), α-CD40 (3/23), α-CD80 (16-10A) (1 (link)), and α-CD86 (GL-1; all from BD Biosciences) mAb and analyzed by flow cytometry on a FACSCalibur or LSR II (BD Biosciences).
Sun C.M., Hall J.A., Blank R.B., Bouladoux N., Oukka M., Mora J.R, & Belkaid Y. (2007). Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid. The Journal of Experimental Medicine, 204(8), 1775-1785.
Publication 2007
alpha HML-1 Cells Centrifugation Chief Cells, Gastric Deoxyribonucleases Edetic Acid Erythrocytes Flow Cytometry Fluorescent Dyes ITGAM protein, human Liberase Lymphocyte Count Nycodenz Osmosis Phenotype
2
Isolation and Culture of Rat Cardiomyocytes
Ventricles were dissected from neonatal (1–2 d) Sprague-Dawley rat hearts and dissociated by serial digestion with 0.4 mg/ml collagenase and 0.6 mg/ml pancreatin sterile digestion buffer (116 mM NaCl, 20 mM HEPES, 0.8 mM Na2HPO4, 5.6 mM glucose, 5.4 mM KCl and 0.8 mM MgSO4, pH 7.35). The first digestion supernatant (5 min, 37°C, 160 cycles/min in a shaking waterbath) was removed and discarded. Cell suspensions from subsequent digestions (20 min, 2×25 min, 20 min, 10 min; 37°C 136 cycles/min shaking) were recovered by centrifugation (5 min, 60×g) and the cell pellet resuspended in plating medium (Dulbecco's modified Eagle's medium (DMEM)/medium 199 [4:1 (v/v)], 15% (v/v) FCS, 100 units/ml penicillin and streptomycin). The cells were pre-plated on plastic tissue culture dishes (30 min) to remove non-cardiomyocytes. For biochemistry and molecular biology experiments, non-adherent viable cardiomyocytes were plated at a density of 4×106 cells/dish on 60 mm Primaria dishes pre-coated with sterile 1% (w/v) gelatin (Sigma-Aldrich UK). After 18 h myocytes were confluent and beating spontaneously. For immunostaining experiments, cardiomyocytes were plated at 1.5×106 cells/dish on 35 mm Primaria dishes containing glass coverslips pre-coated with sterile 1% (w/v) gelatin followed by laminin (20 µg/ml in PBS; Sigma-Aldrich UK). The plating medium was withdrawn and cells were incubated in serum-free maintenance medium (DMEM/medium [4:1 (v/v)], 100 units/ml penicillin and streptomycin) for a further 24 h.
Cardiomyocytes were unstimulated (Controls), exposed to PD184352 (2 µM), a cell-permeable form of the exoenzyme C3 transferase from Clostridium botulinum (C3T; 1 or 5 µg/ml) or ET-1 (100 nM), or exposed to ET-1 following pretreatment with PD184352 (10 min) or C3T (120 min). PD184352 (Alexis Biochemicals, Enzo Life Sciences) was prepared as a stock solution in DMSO (4 mM). Cell-permeable C3T (Cytoskeleton Inc., Cat. no. CT03) was resuspended in 66% (v/v) glycerol (0.02 mg/ml). This represents highly purified C3T linked to a cell penetrating moiety though a disulfide bond that allows rapid uptake into cells. In the cytoplasm, the disulfide bond is reduced and the C3T diffuses through the cell. ET-1 (Bachem UK) was prepared as a stock solution in water (0.1 mM). ET-1, PD184352 or C3T were added directly to the tissue culture medium. Unless otherwise stated, incubation times with ET-1 were dependent upon the time at which individual signaling components are substantially activated: 30 s for RhoA.GTP loading [25] (link), 5 min for activation of ERK1/2 [26] (link) or p38-MAPKs [27] (link), 15 min for activation of JNKs [28] (link) and 1 h for expression of immediate early genes [10] (link). For inhibitor studies, cells were pre-treated with inhibitor for a minimum period required to provide adequate inhibition of the pathways (10 min for PD184352; 2 h for C3T) prior to addition of ET-1 for the appropriate times. Cells were also exposed to inhibitor alone for the full duration of the incubation time. All samples were harvested together at the end of the experiment.
Marshall A.K., Barrett O.P., Cullingford T.E., Shanmugasundram A., Sugden P.H, & Clerk A. (2010). ERK1/2 Signaling Dominates Over RhoA Signaling in Regulating Early Changes in RNA Expression Induced by Endothelin-1 in Neonatal Rat Cardiomyocytes. PLoS ONE, 5(4), e10027.
Full text: Click here
Publication 2010
Buffers Cells Centrifugation Chief Cells, Gastric Clostridium botulinum Collagenase Culture Media Cytoplasm Cytoskeleton Digestion Disulfides Gelatins Genes, Immediate-Early Glucose Glycerin Heart Heart Ventricle HEPES Hyperostosis, Diffuse Idiopathic Skeletal Infant, Newborn Laminin Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinase p38 Muscle Cells Myocytes, Cardiac Pancreatin PD 184352 Penicillins Permeability Psychological Inhibition Rats, Sprague-Dawley RHOA protein, human Serum Sodium Chloride Sterility, Reproductive Streptomycin Sulfate, Magnesium Sulfoxide, Dimethyl Tissues Transferase
3
ChIP-seq Protocol for Nucleosome Mapping
ChIPs were essentially carried out as described (Koch et al. 2011 (link)). All antibodies and ChIP conditions are described in Supplemental Table 1. MNase nucleosome digestion of permeabilized cells was optimized to obtain a majority of mononucleosomes (70%–80% as estimated on a Bioanalyzer DNA chip). The corresponding DNA fraction was selected after library preparation. The sequencing procedure was conducted using at least 1 ng of starting material and run on a Genome Analyzer II (Illumina). The in vivo phenanthroline digestion of nucleosomes was adapted from Tsang et al. (1996) (link) and is described in the Supplemental Methods together with the computational processing and analysis pipeline.
Fenouil R., Cauchy P., Koch F., Descostes N., Cabeza J.Z., Innocenti C., Ferrier P., Spicuglia S., Gut M., Gut I, & Andrau J.C. (2012). CpG islands and GC content dictate nucleosome depletion in a transcription-independent manner at mammalian promoters. Genome Research, 22(12), 2399-2408.
Publication 2012
Antibodies Chief Cells, Gastric Digestion DNA Chips DNA Library Genome Nucleosomes Phenanthrolines
4
Prolonged RNA Extraction from Pelleted Cells
After sorting, cells were pelleted by centrifugation at 3000 g for 5′ at 4°C. The supernatant was discarded. Total RNA was isolated from the pellet using the RecoverAll Total Nucleic Acid Isolation kit (Ambion), starting at the protease digestion stage of manufacturer-recommended protocol. The following modification to the isolation procedure was made: instead of incubating cells in digestion buffer for 15 minutes at 50°C and 15 minutes at 80°C, we carried out the incubation for 3 hours at 50°C. Cell lysates were frozen at −80°C overnight before continuing the RNA isolation by the manufacturer's instructions.
Hrvatin S., Deng F., O'Donnell C.W., Gifford D.K, & Melton D.A. (2014). MARIS: Method for Analyzing RNA following Intracellular Sorting. PLoS ONE, 9(3), e89459.
Full text: Click here
Publication 2014
Buffers Cells Centrifugation Chief Cells, Gastric Digestion Freezing isolation Nucleic Acids Peptide Hydrolases
5
Isolation and Characterization of Murine Periosteal Progenitors
Intact, age-matched mouse tibias were dissected free of surrounding tissues and both ends were removed at the growth plate sites. The remaining bone fragments were digested in 2 mg/mL collagenase A and 2.5 mg/mL trypsin. Cells from the first 3 minutes of digestion were discarded and cells from a subsequent 20 minutes of digestion were seeded in the growth medium (αMEM supplemented with 15% fetal bovine serum [FBS] plus 55 μM β-mercaptoethanol, 2mM glutamine, 100IU/ mL penicillin and 100 μg/mL streptomycin) for periosteal mesenchymal progenitor culture. For colony-forming unit fibroblast (CFU-F) assay, cells were seeded at 0.3 × 106 cells/ T25 flask. Seven days later, flasks were stained with 3% crystal violet to quantify CFU-F numbers. To study the radiation effect on progenitor proliferation, cells were seeded at 5600cells/cm2, irradiated (8Gy) the next day, and counted at 0, 1, 2, 4, and 5 days postradiation. To study the radiation effect on differentiation, cells were irradiated (8 Gy) when confluent, then switched to either osteogenic medium (αMEM with 10% FBS, 10 nM dexamethasone, 10 mM β-glycerophosphate, 50 μg/ mL ascorbic acid, 100IU/mL penicillin, and 100 μg/mL streptomycin) for 2 weeks followed by alizarin staining, or adipogenic medium (αMEM with 10% FBS, 0.5 mM isobutylmethylxanthine, 10 mM indomethacin, 1 μM dexamethasone, 10 μg/mL insulin, 100IU/mL penicillin, and 100 μg/mL streptomycin) for 1 week followed by Oil Red O staining. For chondrogenic differentiation, after radiation progenitor cells were suspended at 1.25 × 106 cells/mL in chondrogenic medium (high-glucose DMEM, 100 μg/mL sodium pyruvate, 1% ITS+Premix, 50 μg/mL ascor-bate-2-phosphate, 40 μg/mL L-proline, 0.1 mM dexamethasone, 10 ng/mL TGF-β3,100 IU/mL penicillin, and 100 μg/mL streptomycin). Aliquots of 200 μL cell solution were then distributed to a V-bottomed 96-well plate followed by centrifugation at 300G for 5 minutes. Pellets were cultured for 3 weeks and then harvested for paraffin sections with Alcian blue staining. To culture cells under hypoxia condition, cell culture plates were placed in a Modular Incubator Chamber (Billups-Rothenberg, Del Mar, CA, USA) supplied with 0.1% oxygen.
Wang L., Tower R.J., Chandra A., Yao L., Tong W., Xiong Z., Tang K., Zhang Y., Liu X.S., Boerckel J.D., Guo X., Ahn J, & Qin L. (2019). Periosteal Mesenchymal Progenitor Dysfunction and Extraskeletally-Derived Fibrosis Contribute to Atrophic Fracture Nonunion. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 34(3), 520-532.
Publication 2019
2-Mercaptoethanol Adipogenesis Alcian Blue alizarin Ascorbic Acid beta-glycerol phosphate Biological Assay Bones Cell Culture Techniques Cells Centrifugation Chief Cells, Gastric Chondrogenesis Dexamethasone Electromagnetic Radiation Epiphyseal Cartilage Fetal Bovine Serum Fibroblasts Glucose Glutamine Hypoxia Indomethacin Insulin Mesenchyma Mus Neutrophil Collagenase Osteogenesis Oxygen Paraffin Pellets, Drug Penicillins Periosteum Phosphates Proline Pyruvate Radiation Effects Sodium Stem Cells Streptomycin Tibia Tissues Trypsin Violet, Gentian

Most recents protocols related to «Chief Cells, Gastric»

1
Total RNA Extraction and Real-Time PCR Analysis
Total RNA extraction, RNA-free centrifuge tubes, EP tubes, PCR tubes, and pipetting nozzles were used in this experiment. The macrophages were washed twice with sterile PBS, the PBS was aspirated, an appropriate amount of ACCUTASE cell digestion solution was added, and the cells were shaken gently and placed in a cell incubator. When the cells gradually fell off the bottle, sterile PBS was added, the adherent cells were blown with a pipette gun, and the cell suspension was centrifuged at 1500 RPM/min for 10 min. The temperature of the water bath was maintained at 70°C. The supernatant was discarded and 300 μL of the lysate was added to the cell precipitate and gently aspirated using a pipette gun. Next, 300 μL of RNA diluent was added, mixed well, and placed in a water bath at 70°C for 3 min to improve the RNA yield. The procedure was as follows: 600 μL of RNA wash solution was added to the column, centrifuge at 12000 RPM/min for 1 min, the filtrate obtained in the collection tube was discarded, and 50 μL DNase I incubation solution was added to the middle of the adsorption membrane of each tube of the centrifugation column and incubated at room temperature for 15 min. Add 600 μL RNA solution, centrifuge at 12000 RPM/min for 1 min, discard the filtrate obtained in the collection tube, and repeat the above procedure twice. The concentration and purity of RNA were determined using an ultramicro spectrophotometer, and the RNA was stored at -80°C until further analysis. The mRNA levels of the target genes were detected by real-time PCR as previously described (27 (link)) and the primers were shown in Table 1.
Zhang M., Liu K., Zhang Q., Xu J., Liu J., Lin H., Lin B., Zhu M, & Li M. (2023). Alpha fetoprotein promotes polarization of macrophages towards M2-like phenotype and inhibits macrophages to phagocytize hepatoma cells. Frontiers in Immunology, 14, 1081572.
Full text: Click here
Publication 2023
accutase Adsorption Bath Cells Centrifugation Chief Cells, Gastric Cocaine Deoxyribonuclease I Genes Macrophage Oligonucleotide Primers Place Cells Real-Time Polymerase Chain Reaction RNA, Messenger Sterility, Reproductive Tissue, Membrane
2
Macrophage Migration Assay Protocol
Macrophages in each group were digested with ACCUTASE cell digestion solution, and the number of cells was adjusted to 1×106/mL using medium without serum. A Transwell chamber (pore size, 8 μm) was used, and 500 μL of fresh RPMI 1640 medium containing 20% FBS was added to each well in the lower chamber. Two hundred microliters of cell suspension were added to each upper chamber, and the cells were cultured for 72 h in a cell incubator. The cells in the upper layer of the Transwell chamber were gently wiped with a cotton ball, washed twice with PBS, and treated with 4% paraformaldehyde for 20 min. The fixed Transwell chamber was cleaned twice with PBS and stained with 0.1% crystal violet for 20 min. After staining, the residual staining solution was cleaned with PBS and the plate was allowed to dry. Images were captured under an inverted microscope, and five fields were randomly selected for each group to calculate the average value.
Zhang M., Liu K., Zhang Q., Xu J., Liu J., Lin H., Lin B., Zhu M, & Li M. (2023). Alpha fetoprotein promotes polarization of macrophages towards M2-like phenotype and inhibits macrophages to phagocytize hepatoma cells. Frontiers in Immunology, 14, 1081572.
Full text: Click here
Publication 2023
accutase Chief Cells, Gastric Gossypium Macrophage Microscopy paraform Serum Violet, Gentian
3
Calculating Bacterial Outer Membrane Length
To calculate OM rest length, we used Morphometrics to measure HADA-labeled cell-envelope contours before and after plasmolysis and FM 4–64-labled OM contours of wall-less spheroplasts induced by lysozyme as described above. For each cell, we calculated the surface area of the turgid cell, Aturgid, from its contour by assuming rotational symmetry around its long axis. After digestion of the cell wall by lysozyme, the OM adopted an amorphous shape, while remaining trapped in the flow cell with height assumed to be equal to the cell width before plasmolysis, w. We calculated the surface area of the OM, Aom, using its 2-dimensional area, a, and contour length, s, via the relation Aom=2a+ws. Finally, to calculate the rest length of the OM, lom, defined as the length of the OM if it were reshaped into a rod cell shape of width w, we used the equation lom=lturgid+(Aom−Aturgid)/(πw). Here, we used approximated a rod-shaped cell as a cylinder capped by two hemispheres. Thus, the difference between the length of a turgid cell and the OM rest length is proportional to the difference between the corresponding surface areas by a factor of 1/(πw), the reciprocal of the cross-sectional perimeter.
Mikheyeva I.V., Sun J., Huang K.C, & Silhavy T.J. (2023). Mechanism of outer membrane destabilization by global reduction of protein content. bioRxiv.
Publication Preprint 2023
Cells Cell Shape Chief Cells, Gastric Epistropheus factor A FM 4-64 Muramidase Perimetry Rod Photoreceptors Spheroplasts
4
MNase-seq Protocol for Chromatin Analysis
Cells were grown for three days after induction with 0.5 mM CuSO4 to induce the expression of either PH-WT or PH-ML. Cells were crosslinked in 1% formaldehyde for 10 min at room temperature, tumbling end over end. Crosslinking was quenched by 1 M glycine (from 2.5 M pH 7.9 stock) and cells were tumbled for 10 min at RT. Cells were resuspended in cold PBS (+PI), pelleted through a sucrose cushion (20% sucrose in PBS), resuspended in PBS, and flash frozen in liquid nitrogen at 107 cells per tube and stored at −80°C. For MNase digestion, the cell pellet was resuspended in PBS with 0.1% Triton-X 100 (PBS-TX). Digestion of 106 cells per titration point took place in a volume of 400 μl PBS-TX supplemented with 1 mM CaCl2. Either 1.5 U, 6.25 U, 25 U, 100 U or 400 U of MNase (Worthington Biochemical) was added to pre-warmed cells and incubated at 37°C for 3 min. Digestion was stopped by moving samples to ice and adding of 10 μl of 250 mM EDTA, 250 mM EGTA, and 10 μl of 20% SDS. For DNA cleanup, the digestion products were incubated with RNase (Roche) for 30 min at 37°C, with proteinase K (2.5 μl of 20 mg/ml) (Roche) for 60 min at 55°C, and incubated at 65°C overnight to reverse crosslinks. After phenol–chloroform extraction and ethanol precipitation, the purified DNA was used as input into the library preparation protocol described in Bowman et al (65 (link)), and then sequenced on a HiSeq-2000 sequencer according to the manufacturer’s instructions for paired-end sequencing.
Amin A., Kadam S., Mieczkowski J., Ahmed I., Bhat Y.A., Shah F., Tolstorukov M.Y., Kingston R.E., Padinhateeri R, & Wani A.H. (2023). Disruption of polyhomeotic polymerization decreases nucleosome occupancy and alters genome accessibility. Life Science Alliance, 6(5), e202201768.
Full text: Click here
Publication 2023
cDNA Library Cells Chief Cells, Gastric Chloroform Cold Temperature Digestion Edetic Acid Egtazic Acid Endopeptidase K Endoribonucleases Ethanol Formaldehyde Freezing Glycine Nitrogen Phenol Sucrose Titrimetry Triton X-100
5
HepG2 cell viability assay with digestion supernatant

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2023
Atmosphere Cell Culture Techniques Cells Chief Cells, Gastric Culture Media Digestion Hep G2 Cells Penicillins Sincalide Streptomycin Sulfoxide, Dimethyl Vision

Top products related to «Chief Cells, Gastric»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Collagenase type 2 by Worthington
Sourced in United States, United Kingdom, Jersey, Germany, Japan, Switzerland, Canada, Australia, France
Collagenase type II is an enzyme used in cell and tissue culture applications. It is responsible for the breakdown of collagen, a structural protein found in the extracellular matrix. This enzyme is commonly used to facilitate the dissociation of cells from tissues during cell isolation and harvesting procedures.
3
Trypsin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Germany, Canada, Japan, France, Australia, Italy, Switzerland, Spain, Brazil, Belgium, Netherlands, Ireland, Singapore, Poland, Macao, India, Sweden, Denmark, Austria, Argentina, Israel, Lithuania
Trypsin is a proteolytic enzyme that hydrolyzes peptide bonds in proteins. It is commonly used in cell biology and molecular biology applications to facilitate cell detachment and dissociation.
4
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
5
Collagenase type 1 by Worthington
Sourced in United States, Germany, Japan, Italy, United Kingdom, Canada, China
Collagenase type I is an enzyme used to digest collagen, which is a key structural component of the extracellular matrix. It is commonly used in cell isolation and tissue dissociation procedures.
6
Dnase 1 by Merck Group
Sourced in United States, Germany, Switzerland, United Kingdom, Italy, Japan, Macao, Canada, Sao Tome and Principe, China, France, Australia, Spain, Belgium, Netherlands, Israel, Sweden, India
DNase I is a laboratory enzyme that functions to degrade DNA molecules. It catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, effectively breaking down DNA strands.
7
Triton x 100 by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, China, Japan, Canada, France, Sao Tome and Principe, Switzerland, Macao, Poland, Spain, Australia, India, Belgium, Israel, Sweden, Ireland, Denmark, Brazil, Portugal, Panama, Netherlands, Hungary, Czechia, Austria, Norway, Slovakia, Singapore, Argentina, Mexico, Senegal
Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.
8
Pancreatin by Merck Group
Sourced in United States, Germany, United Kingdom, Poland, China, Italy, France, Spain, Switzerland, Canada, Australia, India, Ireland, Jersey, Belgium, Sao Tome and Principe, Denmark
Pancreatin is a digestive enzyme complex derived from the pancreas of mammals. It contains a mixture of digestive enzymes, including amylase, lipase, and protease, which play a role in the breakdown of carbohydrates, fats, and proteins, respectively.
9
70 μm cell strainer by BD
Sourced in United States, Germany, United Kingdom, Canada, Japan, Sweden
The 70-μm cell strainer is a laboratory equipment item designed to filter and separate cells. It features a mesh screen with 70-micron pores that allow the passage of single cells while retaining larger cellular aggregates or debris.
10
Collagenase by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, Japan, France, Macao, Switzerland, China, Canada, Australia, Spain, Austria, Sao Tome and Principe, Israel, Belgium, Sweden, Ireland, Jersey, India
Collagenase is an enzyme that breaks down collagen, the primary structural protein found in the extracellular matrix of various tissues. It is commonly used in cell isolation and tissue dissociation procedures.

More about "Chief Cells, Gastric"

Chief cells, also known as principal cells, are the predominant secretory cell type found in the gastric glands of the stomach.
These specialized cells play a crucial role in the process of gastric digestion by producing and secreting pepsinogen, an inactive enzyme precursor that is converted to the active digestive enzyme pepsin when exposed to the acidic environment of the stomach.
The function and regulation of chief cells are essential for understanding gastrointestinal physiology, acid-base balance, and digestive disorders.
Researchers often study chief cells using various techniques, such as isolating them from the stomach using enzymes like Collagenase type II, Trypsin, and Pancreatin, followed by purification steps that may involve a 70-μm cell strainer and DNase I.
The isolated chief cells can then be cultured in media like DMEM and analyzed using techniques like Triton X-100 treatment for permeabilization.
Identifying the most accurate and reliable protocols for studying chief cells and gastric function is crucial for advancing research in this field.
PubCompare.ai's AI-driven platform can help researchers locate the best protocols from the scientific literature, preprints, and patents, optimizing research outcomes and improving reproducibility.
By leveraging advanced comparison tools, researchers can discover the most reliable and efficient methods for investigating chief cells and gastric physiology, contributing to a deeper understanding of this important aspect of digestive system function.