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Cumulus Cells

Cumulus Cells: Specialised cells that surround and support the oocyte within the ovarian follicle.
They play a crucial role in oocyte maturation, ovulation, and fertilization.
Cumulus cells provide nutrients, signaling molecules, and physical support to the developing oocyte, ensuring its proper growth and development.
Optimizing research protocols for Cumulus Cells is essential for advancing our understanding of reproductive biology and improving assisted reproductive technologies.
PubCompare.ai can help researchers easily find, compare, and select the most appropriate Cumulus Cell protocols from literature, preprints, and patents, enhancing reproducibility and accuracy in their studies.

Most cited protocols related to «Cumulus Cells»

1
Porcine Somatic Cell Nuclear Transfer
SCNT was performed as previously described [23] (link), [25] (link). After culturing for 38 h to 42 h, oocytes with expanded cumulus cells were briefly treated with 0.1% (w/v) hyaluronidase and denuded of cumulus cells using a finely drawn glass capillary pipette. Oocytes extruding the first polar body with uniform cytoplasm were cultured in NCSU23 medium supplemented with 0.1 µg/mL demecolcine, 0.05 M sucrose, and 4 mg/mL bovine serum albumin (BSA) for 0.5 h to 1 h. The oocytes were enucleated by aspirating the first polar body and adjacent cytoplasm using a bevelled pipette (approximately 20 µm in diameter) in Tyrode’s lactate medium supplemented with 10 µM hydroxyethyl piperazineethanesulfonic acid (HEPES), 0.3% (w/v) polyvinylpyrrolidone, and 10% FBS in the presence of 0.1 µg/mL demecolcine and 5 µg/mL cytochalasin B. Any protrusion observed on the surface of an oocyte was removed along with the polar body. Fetal, newborn, and adult fibroblasts of the fourth to ninth passages were used as nuclear donors after cell cycle synchronization by 0.5% FBS serum starvation for 48 h. A single donor cell was inserted into the perivitelline space of an enucleated oocyte.
Donor cells were fused with the recipient cytoplasts with a single direct current pulse of 200 V/mm for 20 µs using an embryonic cell fusion system (ET3, Fujihira Industry Co. Ltd., Tokyo, Japan) in fusion medium [0.25 M d-sorbic alcohol, 0.05 mM Mg(C2H3O2)2, 20 mg/mL BSA, and 0.5 mM HEPES (free acid)]. The reconstructed embryos were cultured for 2 h in PZM-3 and then activated with a single pulse of 150 V/mm for 100 µs in an activation medium containing 0.25 M d-sorbic alcohol, 0.01 mM Ca(C2H3O2)2, 0.05 mM Mg(C2H3O2)2, and 0.1 mg/mL BSA. The reconstructed embryos were equilibrated in PZM-3 supplemented with 5 µg/mL cytochalasin B for 2 h at 38.5°C in humidified atmosphere of 5% CO2, 5% O2, and 90% N2 (APM-30D, ASTEC, Japan).
Wei H., Qing Y., Pan W., Zhao H., Li H., Cheng W., Zhao L., Xu C., Li H., Li S., Ye L., Wei T., Li X., Fu G., Li W., Xin J, & Zeng Y. (2013). Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells. PLoS ONE, 8(2), e57728.
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Publication 2013
Acids Adult Atmosphere Capillaries Cell Cycle Cells Cumulus Cells Cytochalasin B Cytoplasm Demecolcine Donors Embryo Ethanol Fetus Fibroblasts Fusions, Cell Hyaluronidase Infant, Newborn Lactate Oocytes Polar Bodies Povidone Pulse Rate Serum Serum Albumin, Bovine Sucrose Tissue Donors
2
Mitochondrial Isolation from IVM Oocytes
Mitochondrial isolation from in vitro matured metaphase II BCB+ oocytes was performed using a drill-fitted Teflon pestle57 (link). Briefly, after hyaluronidase treatment, mechanical stripping and multiple PBS-washes to eliminate all cumulus cells, denuded oocytes were resuspended in 5 mL mitochondrial isolation buffer +2 mg/ml BSA (20 mM Hepes pH 7.6, 220 mM Mannitol, 70 mM sucrose, 1 mM EDTA) and homogenised by no more than 10 strokes of the pestle, at 4 °C or on ice. The oocyte homogenate was centrifuged at 800 g for 10 minutes to remove cell debris and the supernatant was centrifuged at 10,000 g for 20 minutes to pellet the mitochondrial fraction. The pellet was resuspended in isolation buffer without BSA then centrifuged at 10,000 g for 20 minutes. The supernatant was removed and the mitochondrial pellet resuspended in isolation buffer without BSA. The mitochondrial suspension was further concentrated by transferring it to a sealed straw (Flexipet, Cook Australia) followed by centrifugation for 25 seconds at 10,000 g. The mitochondrial suspension was dispensed onto an ICSI plate. At injection, a single sperm along with 3pl of mitochondrial isolate was drawn up into the injection pipette. The 3 pl of mitochondrial isolate along with the sperm was injected into the oocyte.
The mitochondrial isolate was quantified for mtDNA copy number by injecting an identical volume to that injected into oocytes into 2 μl of H2O within a PCR tube, and analysed in triplicate by real time PCR using mtDNA-specific primers, as described in ‘Analysis of mtDNA copy number’. Furthermore, the non-concentrated mitochondrial suspension (10 μl) underwent respiration analysis, as described in ‘Mitochondrial respiration analysis’.
Cagnone G.L., Tsai T.S., Makanji Y., Matthews P., Gould J., Bonkowski M.S., Elgass K.D., Wong A.S., Wu L.E., McKenzie M., Sinclair D.A, & John J.C. (2016). Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency. Scientific Reports, 6, 23229.
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Publication 2016
Buffers Cell Respiration Cells Centrifugation Cerebrovascular Accident Cumulus Cells DNA, Mitochondrial Drill Edetic Acid G-800 HEPES Hyaluronidase isolation Mannitol Metaphase Mitochondrial Inheritance Neoplasm Metastasis Oligonucleotide Primers Oocytes Real-Time Polymerase Chain Reaction Sperm Sperm Injections, Intracytoplasmic Sucrose Teflon
3
Mouse Embryo Genome Editing
We used ICR and B6D2F1 (C57BL/6 x DBA2 F1) female mice in this study. The ICR strain was mainly used for determining the optimal conditions for electroporation, and the B6D2F1 strain was used for genome editing.
Fertilized eggs were collected from the oviducts of E0.5 (12 hours after the midpoint of the day of vaginal plug) ICR or B6D2F1 females naturally intercrossed with males of the same strain. The covering cumulus cells were removed by incubating in 1% hyaluronidase/M2 medium (Sigma). For the genome editing experiments targeting H2b-mCherry, the eggs were obtained from B6D2F1 females intercrossed with R26-H2b-mCherry males. The collected eggs were pre-cultured in mWM medium until electroporation.
Hashimoto M, & Takemoto T. (2015). Electroporation enables the efficient mRNA delivery into the mouse zygotes and facilitates CRISPR/Cas9-based genome editing. Scientific Reports, 5, 11315.
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Publication 2015
Anemia, Diamond-Blackfan, 2 Culture Media Cumulus Cells Eggs Electroporation Therapy Females Hyaluronidase Males Mice, House Oviducts Strains Vagina Zygote
4
Porcine Oocyte Isolation and Culture
Porcine ovaries were collected from Hongteng slaughterhouse (Chenggong Ruide Food Co., Ltd, Kunming, Yunnan Province, China) with the permission to use animal parts for this study. The ovaries were transported to the laboratory at 25°C to 30°C in 0.9% (w/v) NaCl solution supplemented with 75 mg/mL potassium penicillin G and 50 mg/mL streptomycin sulfate. Cumulus-oocyte complexes were obtained from follicles 3 mm to 6 mm in diameter using an 18-gauge needle connected to a 10 mL disposable syringe. Cumulus-oocyte complexes with at least three layers of compacted cumulus cells were selected, and approximately 50 oocytes were cultured in 200 µL drops of TCM-199 medium supplemented with 0.1 mg/mL pyruvic acid, 0.1 mg/mL l-cysteine hydrochloride monohydrate, 10 ng/mL epidermal growth factor, 10% (v/v) porcine follicular fluid, 75 mg/mL potassium penicillin G, 50 mg/mL streptomycin sulfate, and 10 IU/mL eCG and hCG (Teikoku Zouki Co., Tokyo, Japan) at 38.5°C in an atmosphere with 5% CO2 (100% humidity) (APC-30D, ASTEC, Japan).
Wei H., Qing Y., Pan W., Zhao H., Li H., Cheng W., Zhao L., Xu C., Li H., Li S., Ye L., Wei T., Li X., Fu G., Li W., Xin J, & Zeng Y. (2013). Comparison of the Efficiency of Banna Miniature Inbred Pig Somatic Cell Nuclear Transfer among Different Donor Cells. PLoS ONE, 8(2), e57728.
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Publication 2013
Animals Atmosphere Cumulus Cells Cysteine Hydrochloride Epidermal growth factor Follicular Fluid Food Hair Follicle Humidity Needles Oocytes Ovary Penicillin G Potassium Pigs Pyruvic Acid Sodium Chloride Streptomycin Sulfate Syringes
5
Porcine Oocyte Maturation and Embryo Collection

In vitro maturation (IVM) was performed according to established methods [26] (link). Briefly, porcine ovaries were collected from a local slaughterhouse and kept in saline at 32–37°C. Antral follicles (3–5 mm in diameter) were aspirated with an 18-gauge needle. Aspirated oocytes with an evenly granulated cytoplasm and three uniform layers of compact cumulus cells were selected and cultured in four-well plates containing 500 µl of TCM199 (Gibco) based medium for 42 h. At the end of IVM, the cumulus cells of cumulus oocyte complexes (COCs) were removed by vortexing for 2 m in TCM199 based medium supplemented with 0.5% hyaluronidase (Sigma).
Oocytes at the germinal vesicle (GV) and MII stages and preimplantation embryos were collected. For preimplantation embryos, one-cell, two-cell, four-cell, 8-cell, morula and blastocyst stage embryos were collected at 6 h, 24 h, 48 h, 72 h, 120 h and 168 h post-activation, respectively. To exclude the possible contamination of remaining cumulus cells or sperm, the zona pellucida was removed by treatment with warm Tyrode's acidic solution for 20 sec.
Wei Y., Huan Y., Shi Y., Liu Z., Bou G., Luo Y., Zhang L., Yang C., Kong Q., Tian J., Xia P., Sun Q.Y, & Liu Z. (2011). Unfaithful Maintenance of Methylation Imprints Due to Loss of Maternal Nuclear Dnmt1 during Somatic Cell Nuclear Transfer. PLoS ONE, 6(5), e20154.
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Publication 2011
Acids Blastocyst Cells Cumulus Cells Cytoplasm Embryo Graafian Follicle Hyaluronidase Morula Needles Oocytes Ovary Pigs Saline Solution Sperm Tyrode's solution Zona Pellucida

Most recents protocols related to «Cumulus Cells»

1
Bovine Embryo In Vitro Production
In vitro production of bovine embryos, including in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC), was performed as published before (Cui et al. 2016 (link), Li et al. 2021 (link), Shi et al. 2021 (link)). Briefly, cumulus–oocyte complexes (COCs) containing intact cumulus cells were collected from bovine ovaries obtained from a local abattoir and these COCs were matured in the IVM medium at 38.5°C under 5% CO2 in humidified air for 22–24 h. The IVM medium consists of 10% fetal bovine serum (FBS; Gibco-BRL), 1 IU/mL follicle-stimulating hormone (Sansheng Biological Technology, Ningbo, China), 0.1 IU/mL luteinizing hormone (Solarbio, Beijing, China), 1 mM sodium pyruvate (Thermo Fisher Scientific), 2.5 mM GlutaMAX™ (Thermo Fisher Scientific), and 10 μg/mL gentamicin in Medium-199 (M4530). After maturation, COCs (60–100 COCs per well in 4-well plates) were then incubated with spermatozoa (1 × 106–5 × 106) purified from frozen-thawed semen using a percoll gradient in BO-IVF medium (IVF Bioscience, Falmouth, Cornwall, UK). The IVF condition was 38.5°C under 5% CO2 for 9–12 h. Putative zygotes were then removed from cumulus cells by pipetting up and down in Medium-199 (M7528) supplemented with 2% FBS (Gibco-BRL). Embryos were incubated in BO-IVC medium (IVF Bioscience) at 38.5°C under 5% CO2 in humidified air until use.
Shi Y., Hu B., Wang Z., Wu X., Luo L., Li S., Wang S., Zhang K, & Wang H. (2023). Functional role of GATA3 and CDX2 in lineage specification during bovine early embryonic development. Reproduction (Cambridge, England), 165(3), 325-333.
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Publication 2023
Biopharmaceuticals Bos taurus Cumulus Cells Embryo Fertilization in Vitro Frozen Semen Gentamicin Human Follicle Stimulating Hormone Luteinizing hormone Oocytes Ovary Percoll Pyruvate Sodium Sperm Zygote
2
Bovine Oocyte In Vitro Maturation Protocol
For in vitro maturation (IVM), bovine ovaries, collected from a local slaughterhouse, were kept in normal saline and transported to the laboratory within 2 h. Once in the laboratory, ovaries were thoroughly washed with 70% ethanol and PBS solution to avoid contamination. Subsequently, cumulus-oocyte complexes (COCs) with a diameter of about 100–130 μm and a central oocyte surrounded by at least three layers of cumulus cells were collected according to the department's protocols (32 (link)).
After collection, COCs were transferred to 500 μl of IVM droplets (40–50 COCs per droplet) covered by mineral oil and cultured for a subsequent 24 h in a CO2 incubator (38.5°C and 5% CO2). IVM medium applied in this study was TCM-199 medium supplemented with 0.3 mM sodium pyruvate, 1 μg/ml β-2-oestradiol, 2 mM GlutaMAX, 10 ng/ml EGF, 10% fetal bovine serum, 10 U/ml FSH, 10 U/ml LH and 100 μM cysteamine (67 (link)–69 (link)).
Li S., Ren J., Zhang W., Wang B., Ma Y., Su L., Dai Y, & Liu G. (2023). Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen. Frontiers in Veterinary Science, 10, 1093274.
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Publication 2023
Bos taurus Cumulus Cells Cysteamine Estradiol Ethanol Fetal Bovine Serum Normal Saline Oil, Mineral Oocytes Ovary Pyruvate Sodium
3
Minimal Stimulation IVF with Clomiphene Citrate
A detailed protocol for minimal stimulation with CC has been reported previously.21 (link), 22 , 23 (link) Briefly, CC (50–100 mg/d) (Fuji Pharma Co, Ltd, Tokyo, Japan) was administered orally on an extended regimen from day 3 of the retrieval cycle to the day before induction of final oocyte maturation. EMT was measured using an ultrasound on the day of the trigger. When the dominant follicle developed to a size >18 mm, ovulation triggering was performed using a nasal spray containing the gonadotropin-releasing hormone agonist buserelin (Suprecur; Mochida Pharmaceutical Co, Ltd, Tokyo, Japan; or Buserecur, Fuji Pharma Co, Ltd).24 (link)
At 34 to 36 hours after triggering, oocyte retrieval was performed using a 21-gauge needle (Kitazato Corporation, Shizuoka, Japan), generally without anesthesia or follicular flushing.25 (link) Cumulus-oocyte complexes (COCs) were collected, washed, and transferred to a human tubal fluid (HTF) medium (Kitazato Corporation) with paraffin oil at 5% CO2 in air at 37°C for culturing. Conventional IVF was performed 3 hours later. In cases of intracytoplasmic sperm injection (ICSI), denudation was performed at 4 hours after oocyte retrieval.26 (link),27 (link) For ICSI, cumulus cells surrounding the oocytes were removed, and the denuded oocytes were cultured in the HTF medium covered with paraffin oil for 1 hour before ICSI.
Sperm samples were collected by ejaculation and washed by centrifugation through 70% and 90% density gradients (Isolate; Irvine Scientific, Santa Ana, CA). The prepared sperm were cultured in HTF medium at 5% CO2 in air at 37°C until use.
Nishii S., Ezoe K., Nishihara S., Onogi S., Takeshima K., Karakida S., Fukuda J, & Kato K. (2023). Association between endometrial thickness before ovulation, live birth, and placenta previa rates in clomiphene citrate-treated cycles. AJOG Global Reports, 3(1), 100161.
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Publication 2023
Anesthesia Buserelin Centrifugation Culture Media Cumulus Cells Ejaculation Gonadorelin Homo sapiens Nasal Sprays Needles Oocyte Retrieval Oocytes Ovarian Follicle Ovulation paraffin oils Pharmaceutical Preparations Sperm Sperm Injections, Intracytoplasmic Suprecur Treatment Protocols Ultrasonics
4
Conventional IVF and ICSI Protocols
For conventional IVF, HTF medium supplemented with 10% serum substitute (Irvine Scientific) was used as the fertilization medium.26 (link) COCs were cultured with sperm (100,000 sperm/mL) at 5% CO2 in air at 37°C. Extrusion of the second polar body was confirmed at 5 hours after insemination (day 0) following the removal of cumulus cells. The oocytes were individually cultured in EmbryoSlide (Vitrolife, Göteborg, Sweden), which is suitable for group cultures in 180 µL of medium (ONESTEP medium; Nakamedical Inc, Tokyo, Japan) under paraffin oil. In cases of ICSI, oocytes were inseminated in HEPES-buffered HTF medium.28 (link) Injected oocytes were immediately placed onto EmbryoSlides and cultured individually in 180 µL of medium in paraffin oil. Embryos were cultured at 37°C (gas phase, 5% O2, 5% CO2, and 90% N2) in an Embryoscope+ time-lapse incubator (Vitrolife) for 2 days. Fertilization was observed using the EmbryoViewer software (Vitrolife). Embryo vitrification and warming were performed using Cryotop (Kitazato Corporation) as described previously.29 (link)
Nishii S., Ezoe K., Nishihara S., Onogi S., Takeshima K., Karakida S., Fukuda J, & Kato K. (2023). Association between endometrial thickness before ovulation, live birth, and placenta previa rates in clomiphene citrate-treated cycles. AJOG Global Reports, 3(1), 100161.
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Publication 2023
Cumulus Cells Embryo Embryoscopes Fertilization HEPES Insemination Ovum paraffin oils Polar Bodies Serum Sperm Sperm Injections, Intracytoplasmic Vitrification
5
Porcine Ovary Oocyte Maturation and Analysis
Porcine ovaries were collected from a local abattoir and transported to our laboratory within 2 h in PBS at 4 °C. The immature COCs were aspirated from the follicles with 3–6 mm in diameter, and then 100–120 COCs were cultured in 500 μL TCM-199 medium with EGF (10 ng/mL) for 44 h to acquire the mature COCs. The denuded oocytes (DOs) were obtained by removing the cumulus cells from the mature COCs with 1 mg/mL hyaluronidase. One ampulla at follicular phase was cultured in 200 μL TCM-199 medium, supplemented with 150 immature or mature COCs, 150 DOs, 10 ng/mL TGFB1, and/or 1 μM SD208 (TGFBR1 inhibitor) for 3 h. The ampullae were collected at the end of culture, and the epithelial cells were scraped by the edge of a microscope slide. In some experiments, the epithelial cells were isolated from the ampullae at follicular and corpus rubrum phases. The samples were stored at -80 °C for mRNA and protein analysis.
OOX cumulus cells were produced by microsurgically removing the oocytes, but not the zona pellucida, from the immature COCs. Groups of 100–120 COCs or OOX cumulus cells were cultured in the medium, supplemented with EGF, growth differentiation factor 9 (GDF9, 500 ng/mL, human), bone morphogenetic protein 15 (BMP15, 500 ng/mL, human), and/or fibroblast growth factor 8B (FGF8, 100 ng/mL, human) in 500 μL medium. After 44 h culture, cumulus cells were collected for gene and protein analysis. All the cultures were incubated at 39 °C under 5% CO2.
Wu Z., Li B., Yu K., Zheng N., Yuan F., Miao J., Zhang M, & Wang Z. (2023). The Mature COC Promotes the Ampullary NPPC Required for Sperm Release from Porcine Oviduct Cells. International Journal of Molecular Sciences, 24(4), 3118.
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Publication 2023
BMP15 protein, human Bone Morphogenetic Protein 15 Cumulus Cells Epithelial Cells FGF8 protein, human Fibroblast Growth Factor 8b GDF9 protein, human Genes Hair Follicle Homo sapiens Hyaluronidase Menstrual Cycle, Proliferative Phase Microscopy Oocytes Ovary Pigs Proteins Receptor, Transforming Growth Factor-beta Type I RNA, Messenger TGFB1 protein, human Zona Pellucida

Top products related to «Cumulus Cells»

1
Hyaluronidase by Merck Group
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Hyaluronidase is an enzyme used in laboratory settings. It functions by breaking down hyaluronic acid, a component of the extracellular matrix.
2
Human chorionic gonadotropin (hcg) by Merck Group
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The HCG (Human Chorionic Gonadotropin) is a laboratory equipment product by Merck Group. It is a hormone typically used in various diagnostic and research applications. The core function of HCG is to detect and measure the levels of this hormone in biological samples.
3
M2 medium by Merck Group
Sourced in United States, Germany, United Kingdom, France, Japan
M2 medium is a cell culture media formulation designed for the maintenance and cultivation of mouse embryos. It provides the necessary nutrients and growth factors to support the development and growth of mouse embryos in an in vitro environment.
4
Tcm 199 by Thermo Fisher Scientific
Sourced in United States, Spain, Canada, Denmark, Belgium, Japan
TCM-199 is a cell culture medium developed for the in vitro cultivation of various cell types, including mammalian cells. It provides the necessary nutrients and growth factors to support cell growth and maintenance. The formulation is designed to maintain the physiological pH and osmolality required for optimal cell performance.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Bovine serum albumin (bsa) by Merck Group
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Bovine serum albumin (BSA) is a common laboratory reagent derived from bovine blood plasma. It is a protein that serves as a stabilizer and blocking agent in various biochemical and immunological applications. BSA is widely used to maintain the activity and solubility of enzymes, proteins, and other biomolecules in experimental settings.
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M16 medium by Merck Group
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M16 medium is a culture medium used for the isolation and cultivation of microorganisms. It provides the necessary nutrients and growth factors for the growth and maintenance of microbial cultures in a laboratory setting.
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Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
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Mineral oil by Merck Group
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Mineral oil is a clear, odorless, and colorless liquid derived from petroleum. It is commonly used as a lubricant, solvent, and base for various personal care and pharmaceutical products.
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Milrinone by Merck Group
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Milrinone is a type of lab equipment manufactured by Merck Group. It is a phosphodiesterase III inhibitor used to improve cardiac contractility and increase cardiac output. The core function of Milrinone is to enhance myocardial function in patients with congestive heart failure.

More about "Cumulus Cells"

Cumulus cells are specialized cells that surround and support the oocyte (egg cell) within the ovarian follicle.
These cells play a crucial role in the maturation, ovulation, and fertilization of the oocyte.
Cumulus cells provide essential nutrients, signaling molecules, and physical support to the developing oocyte, ensuring its proper growth and development.
Optimizing research protocols for cumulus cells is essential for advancing our understanding of reproductive biology and improving assisted reproductive technologies (ART), such as in vitro fertilization (IVF) and embryo transfer.
Some key components and related terms associated with cumulus cell research and protocols include: - Hyaluronidase: An enzyme that can be used to disperse cumulus cells from the oocyte, allowing for easier manipulation and analysis. - HCG (Human Chorionic Gonadotropin): A hormone that can be used to trigger the final maturation and ovulation of the oocyte, in conjunction with cumulus cell support. - M2 medium and TCM-199: Culture media commonly used to support the growth and development of cumulus-oocyte complexes in vitro. - FBS (Fetal Bovine Serum) and Bovine Serum Albumin (BSA): Protein supplements added to culture media to provide additional nutrients and support for the cumulus-oocyte complex. - M16 medium: Another culture medium used for the in vitro maturation and culture of cumulus-oocyte complexes. - Penicillin/Streptomycin: Antibiotics added to culture media to prevent microbial contamination and support the health of the cumulus-oocyte complex. - Mineral oil: A protective layer added to culture media to prevent evaporation and maintain optimal conditions for the cumulus-oocyte complex. - Milrinone: A compound that can be used to temporarily arrest the maturation of the oocyte, allowing for more precise control over the timing of cumulus cell interactions and oocyte development.
PubCompare.ai can help researchers easily find, compare, and select the most appropriate cumulus cell protocols from literature, preprints, and patents, enhancing the reproducibility and accuracy of their studies in this crucial area of reproductive biology and assisted reproductive technologies.