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Erythroid Cells

Erythroid cells, also known as red blood cells or erythrocytes, are the most abundant type of blood cells in the human body.
These specialized cells are responsible for carrying oxygen from the lungs to the body's tissues and carbon dioxide back to the lungs.
Erythroid cells are produced in the bone marrow through a process called erythropoiesis, which is regulated by the hormone erythropoietin (EPO).
They lack a nucleus and organelles, and their cytoplasm is filled with hemoglobin, a protein that binds to oxygen.
Erythroid cells play a crucial role in maintaining the body's oxygenation and are essential for overall health and well-being.
Reserach into the biology and function of these cells is crucial for understanding and treating a variety of hematopoietic disorders.

Most cited protocols related to «Erythroid Cells»

1
Erythroid Differentiation of Progenitor Cells
The immortalized erythroid progenitor cell lines were induced to differentiate into more mature erythroid cells by culture in erythroid differentiation medium; IMDM (Sigma) containing 10% human AB serum (Kohjin Bio, Saitama, Japan or TAKARA BIO), α-tocopherol (20 ng/ml; Sigma), linoleic acid (4 ng/ml; Sigma), cholesterol (200 ng/ml; Sigma), sodium selenite (2 ng/ml; Sigma), holo-transferrin (200 µg/ml; Sigma), human insulin (10 µg/ml; Sigma), ethanolamine (10 µM; Sigma), 2-ME (0.1 mM; Sigma), D-mannitol (14.57 mg/ml; Sigma), mifepristone (an antagonist of glucocorticoid receptor, 1 µM; Sigma) and EPO (5 IU/ml).
Kurita R., Suda N., Sudo K., Miharada K., Hiroyama T., Miyoshi H., Tani K, & Nakamura Y. (2013). Establishment of Immortalized Human Erythroid Progenitor Cell Lines Able to Produce Enucleated Red Blood Cells. PLoS ONE, 8(3), e59890.
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Publication 2013
alpha-Tocopherol Cell Lines Cholesterol Culture Media Erythroid Cells Ethanolamines Homo sapiens Insulin Linoleic Acid Mannitol Mifepristone NR3C1 protein, human Selenite, Sodium Serum Transferrin
2
Cell Labeling and Trafficking Analysis
C57BL/6J and tcrα−/− mice were purchased from The Jackson Laboratory and breeding colonies were maintained in our animal facility according to institutional animal care and use guidelines. Cells to be labeled were either LN (axillary, brachial, inguinal, and mesenteric) cells from C57BL/6J mice, splenocytes from tcrα−/− mice (after erythroid cell lysis and washing as described earlier), or preactivated T cells. The latter were prepared by culturing LN and spleen cells from C57BL/6J mice (after erythroid cell lysis and washing as described above) for 3 d at 5 × 106 cells/ml in DMEM supplemented with 2 mM glutamine, 50 μM 2-mercaptoethanol, 100 IU/ml penicillin, 0.1 mg/ml streptomycin, 5% FCS, 1 μg/ml anti-CD3 antibody (clone 2C11; Southern Biotechnology Associates, Inc.), and 50 U/ml IL-2. Cells were then washed and rested for a further 3 d in the same medium but without anti-CD3 antibody. Finally, dead cells were removed by centrifugation through lymphocyte separation medium (BioWhittaker).
Cells were labeled with 1 μM CMFDA succinimidyl ester (Molecular Probes) in PBS/0.1% BSA for 5 min at 37°C, and then washed three times with PBS by centrifugation at 1,000 rpm for 5–10 min. Finally, labeled cells were made to 2 × 108/ml in PBS and each 8–10-wk-old recipient was given 0.1 ml intravenously. 2 h later, peripheral blood, spleen, PLNs (axillary, brachial, and inguinal LNs), MLNs, and BM (femurs and tibias) were harvested for fluorocytometry as described above, with 250,000 events being counted per sample.
Koni P.A., Joshi S.K., Temann U.A., Olson D., Burkly L, & Flavell R.A. (2001). Conditional Vascular Cell Adhesion Molecule 1 Deletion in Mice: Impaired Lymphocyte Migration to Bone Marrow. The Journal of Experimental Medicine, 193(6), 741-754.
Publication 2001
2-Mercaptoethanol 5-chloromethylfluorescein diacetate Animals Antibodies, Anti-Idiotypic Arm, Upper BLOOD Cells Centrifugation Clone Cells Erythroid Cells Esters Femur Glutamine Groin Lymphocyte Mesentery Mice, Inbred C57BL Molecular Probes Mucocutaneous Lymph Node Syndrome Mus Penicillins Somatostatin-Secreting Cells Spleen Streptomycin T-Lymphocyte Tibia
3
Immuno-profiling of AML patient samples
Data from six samples were used: two healthy controls, AML027 pre- and post-transplant, and AML035 pre- and post-transplant. Each sample was downsampled to ∼10k confidently mapped reads per cell. Then the gene-cell barcode matrix from each sample was concatenated. PCA, tSNE and k-means clustering were performed on the pooled matrix, following the same steps outlined in PCA and tSNE analysis of PBMCs. For k-means clustering, K=10 was used based on the bend in the sum of squared error scree plot.
Cluster-specific genes were identified following the steps outlined in ‘Identification of cluster-specific genes and marker-based classification'. Classification was assigned based on cluster-specific genes, and based on expression of some well-known markers of immune cell types. ‘Blasts and Immature Ery 1' refers to cluster 4, which expresses CD34, a marker of hematopoietic progenitors39 (link), and Gata2, a marker for early erythroids40 (link). ‘Immature Ery 2' refers to clusters 5 and 8, which show expression of Gata1, a transcription factor essential for erythropoiesis41 (link), but not CD71, which are often found in more committed erythroid cells39 (link). ‘Immature Ery 3' refers to cluster 1, which show expression of CD71. ‘Mature Ery' refers to cluster 2. HBA1, a marker of mature erythroid cells, is preferentially detected in cluster 2. Cluster 3 was assigned as ‘Immature Granulocytes' because of the expression of early granulocyte markers such as AZU1 and IL8 (ref. 42 (link)), and the lack of expression of CD16. Cluster 7 was assigned as ‘Monocytes' because of the expression of CD14 and FCN1, for example. ‘B' refers clusters 6 and 9 because of markers such as CD19 and CD79A. ‘T' refers to cluster 10, because of markers such as CD3D and CD8A.
Zheng G.X., Terry J.M., Belgrader P., Ryvkin P., Bent Z.W., Wilson R., Ziraldo S.B., Wheeler T.D., McDermott G.P., Zhu J., Gregory M.T., Shuga J., Montesclaros L., Underwood J.G., Masquelier D.A., Nishimura S.Y., Schnall-Levin M., Wyatt P.W., Hindson C.M., Bharadwaj R., Wong A., Ness K.D., Beppu L.W., Deeg H.J., McFarland C., Loeb K.R., Valente W.J., Ericson N.G., Stevens E.A., Radich J.P., Mikkelsen T.S., Hindson B.J, & Bielas J.H. (2017). Massively parallel digital transcriptional profiling of single cells. Nature Communications, 8, 14049.
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Publication 2017
cationic antimicrobial protein CAP 37 CD79A protein, human Cells Cytosol Decompression Sickness Erythroid Cells GATA1 protein, human GATA2 protein, human Gene Clusters Genes Grafts Granulocyte Hematopoietic System Hemoglobin A, Glycosylated Monocytes TFRC protein, human Transcription Factor
4
Fluorescent Labeling of Endothelial Cells

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Publication 2013
Alexa594 alexa fluor 488 Alexa Fluor 647 Antibodies cadherin 5 Cells Collagenase Deoxyribonucleases dispase II Dyes Erythroid Cells Flow Cytometry Hematopoietic System Immunoglobulins Institutional Animal Care and Use Committees Isolectins ITGAM protein, human Microscopy Microspheres Molecular Probes Mus Organ Harvesting paraform Sucrose vegfr3 protein, human
5
Lentiviral Transduction of CD34+ Cells for Erythroid Differentiation

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Publication 2018
beta-Globins BLOOD Cells Culture Media, Serum-Free Dexamethasone Diabetes Mellitus Digestive System Donors Erythroid Cells Estradiol Ethics Committees, Research FER protein, human Fibronectins Genes Globin Granulocyte Colony-Stimulating Factor Heart HIV-1 Homo sapiens Insulin Kidney Diseases Ligands Lipids Lung MPL protein, human Mus Osteocytes Patients Quercus retronectin Serum Stem Cells Thrombopoietin Tissue Donors Transferrin Virus

Most recents protocols related to «Erythroid Cells»

1
Bone Marrow Endothelial Cell Isolation
CD31hiEMCNhi cells in the femur and tibia were measured by flow cytometry as previously described (38 (link)). Briefly, we dissected the bones, made small cuts at both sides, and placed them in the sectioned tips within the centrifuge tubes. Then, the tubes were centrifuged at 10,000g for 15 s to get whole bone marrow cells. After ACK lysis, cells were incubated with phycoerythrin/Cy7 anti-mouse CD31 antibody (BioLegend), EMCN monoclonal antibody (eBioscience), fluorescein isothiocyanate (FITC) anti-mouse TER-119/erythroid cell antibody (BioLegend), and FITC anti-mouse CD45 antibody (BioLegend). Cell sorting was performed with CyAn ADP Analyzer (Beckman Coulter), and CD31+CD45−Ter119− cells were considered bone marrow endothelial cells.
Zhang Z., Ding P., Meng Y., Lin T., Zhang Z., Shu H., Ma J., Cohen Stuart M., Gao Y., Wang J, & Zhou X. (2023). Rational polyelectrolyte nanoparticles endow preosteoclast-targeted siRNA transfection for anabolic therapy of osteoporosis. Science Advances, 9(10), eade7379.
Publication 2023
Antibodies, Anti-Idiotypic Bone Marrow Cells Bones Cells Endothelium Erythroid Cells Femur Flow Cytometry Fluorescein isothiocyanate Monoclonal Antibodies Mus Phycoerythrin Tibia
2
Quantification of Hemoglobin Variants
The remaining erythroid cells on day 14 were all collected and washed once with 1X DPBS. The cell pellet was lyzed for hemoglobin measurement by HPLC using the VARIANT™ II β-thalassemia Short Program (Biorad, Hercules, CA, USA). This cation exchange HPLC technique uses an increasing sodium phosphate gradient buffer to separate different hemoglobin variants. Hemoglobin was measured using absorbance at 415 nm, and correction for turbidity was done by the absorbance at 690 nm [21 (link)]. Each 6.5-minute assay detects the most prevalent aberrant hemoglobin variations while also providing quantitative findings for the percentages of HbA2/E and HbF.
Chumchuen S., Sripichai O., Jearawiriyapaisarn N., Fucharoen S, & Peerapittayamongkol C. (2023). Induction of fetal hemoglobin: Lentiviral shRNA knockdown of HBS1L in β0-thalassemia/HbE erythroid cells. PLOS ONE, 18(3), e0281059.
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Publication 2023
Biological Assay Buffers Cells Erythroid Cells Hemoglobin Hemoglobin A2 High-Performance Liquid Chromatographies sodium phosphate Thalassemia
3
Erythroid Cell Morphology Evaluation
Cell morphology was evaluated from 5X104 of erythroid cells. The cells were spun by cytospin (StatSpin CytoFuge 2, Beckman Culter, Inc., CA, USA), stained with modified Wright-Giemsa dye (Sigma-Aldrich, Inc., MO, USA) for 5 minutes, and washed twice with water for two minutes. The slides were dried and cleaned with 70% ethanol before cell morphology observation under an Olympus CX31 light microscope.
Chumchuen S., Sripichai O., Jearawiriyapaisarn N., Fucharoen S, & Peerapittayamongkol C. (2023). Induction of fetal hemoglobin: Lentiviral shRNA knockdown of HBS1L in β0-thalassemia/HbE erythroid cells. PLOS ONE, 18(3), e0281059.
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Publication 2023
Cells Erythroid Cells Ethanol Light Microscopy
4
Protein Expression Analysis of HBS1L
According to the manufacturer’s protocol, approximately 1X106 erythroid cells were collected for protein extraction by NE-PER (Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific, Inc, MA, USA). Protein concentrations were measured by Quick Start Bradford Protein Assay (Bio-Rad Laboratories, Inc.). The extracts were electrophoresed onto 12% SDS-polyacrylamide gel, transferred to polyvinylidene difluoride (PVDF) membrane, and blocked with 5% skim milk. The membranes were reacted with HBS1L specific primary antibody (NBP1-85123; Novus Biologicals, CO, USA), followed by goat anti-rabbit secondary antibody conjugated with horseradish peroxidase (ab97051; Abcam, Cambridge, UK). Enhanced chemiluminescence (ECL; Amersham GE Healthcare, Little Chalfont, UK) was utilized as a substrate for protein visualization by Azure™ c400 Imaging System (Azure Biosystems, Inc., CA, USA). Anti-actin (ab49900) 1:50,000 (Abcam®, Cambridge, UK) and anti-lamin A (L1293) 1:5000 (Sigma-Aldrich, Inc., MO, USA) were utilized as cytoplasmic and nuclear loading controls, respectively.
Chumchuen S., Sripichai O., Jearawiriyapaisarn N., Fucharoen S, & Peerapittayamongkol C. (2023). Induction of fetal hemoglobin: Lentiviral shRNA knockdown of HBS1L in β0-thalassemia/HbE erythroid cells. PLOS ONE, 18(3), e0281059.
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Publication 2023
Actins Antibodies, Anti-Idiotypic Azure A Biological Assay Biological Factors Chemiluminescence Cytoplasm Erythroid Cells Goat Horseradish Peroxidase Immunoglobulins Lamin Type A Milk, Cow's Novus polyacrylamide gels polyvinylidene fluoride Proteins Rabbits Tissue, Membrane
5
Isolation and Purification of Murine Megakaryocyte-Erythroid Progenitors
Female C57BL/6 or GFP mice (8–10 weeks of age) were sacrificed, and femurs and tibiae were flushed with sterile PBS to obtain bone marrow. Subsequently, bone marrow cells were filtered through a 70 µm pore size cell strainer (BD Biosciences, Bedford, MA). Red blood cells (RBC) were lysed using a sterile lysis buffer (8.26 g/L ammonium chloride, 1 g/L sodium bicarbonate and 0.01 M EDTA). Lineage-positive (Lin + ) cells were isolated by magnetic-activated cell sorting beads (MACS) using the MagCellect mouse hematopoietic cell lineage depletion kit in accordance with the manufacturer’s protocol (R&D Systems, Minneapolis, MN)s. The Lin+ cells were stored and used separately. MEPs were isolated from the remaining Lin- cells using erythroid membrane-associated protein (ERMAP)-biotin antibody (Bioss antibodies, bs-12333R-Biotin). ERMAP-positive cells were collected and re-suspended in sterile PBS. In some experiments, Lin- cells depleted from MEPs were used. The depletion was carried out by magnetic beads conjugated with ERMAP biotinylated antibody. The cell populations were used for different purposes as described below.
Vorontsova A., Cooper T.J., Haj-Shomaly J., Benguigui M., Levin S., Manobla B., Menachem R., Timaner M., Raviv Z, & Shaked Y. (2023). Chemotherapy-induced tumor immunogenicity is mediated in part by megakaryocyte-erythroid progenitors. Oncogene, 42(10), 771-781.
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Publication 2023
Antibodies Bicarbonate, Sodium Biotin Bone Marrow Bone Marrow Cells Buffers Cells Chloride, Ammonium CTSL protein, human Edetic Acid Erythrocytes Erythroid Cells Females Femur Hematopoietic System Immunoglobulins Membrane Proteins Mus Population Group Sterility, Reproductive Strains Tibia

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1
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The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
2
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The FACSAria III is a high-performance cell sorter designed for advanced flow cytometry applications. It features a robust and flexible optical system, enabling precise cell sorting and analysis. The core function of the FACSAria III is to provide users with the ability to sort and analyze complex cell samples with high accuracy and efficiency.
3
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FACSDiva software is a user-friendly flow cytometry analysis and data management platform. It provides intuitive tools for data acquisition, analysis, and reporting. The software enables researchers to efficiently process and interpret flow cytometry data.
4
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The FACSAria is a flow cytometry instrument manufactured by BD. It is used for the analysis and sorting of cells and other particles. The FACSAria is designed to provide high-performance cell sorting capabilities, enabling researchers to isolate specific cell populations for further analysis or experimentation.

More about "Erythroid Cells"

Erythroid cells, also known as red blood cells (RBCs) or erythrocytes, are the most abundant type of blood cells in the human body.
These specialized cells play a crucial role in the body's oxygenation by transporting oxygen from the lungs to the tissues and carbon dioxide back to the lungs.
Erythroid cells are produced in the bone marrow through a process called erythropoiesis, which is regulated by the hormone erythropoietin (EPO).
Erythroid cells are unique in that they lack a nucleus and organelles, and their cytoplasm is filled with hemoglobin, a protein that binds to oxygen.
This specialized structure allows them to efficiently carry and deliver oxygen throughout the body.
Researchers studying erythroid cells often utilize tools such as the FACSCalibur, FACSAria III, FACSDiva software, FACSCanto II, and FACSAria II flow cytometers to analyze and sort these cells.
The Hoechst 33342 dye is commonly used to stain and identify erythroid cells.
Additionally, the IMDM culture medium is frequently used for erythroid cell culture and expansion.
The FlowJo software is a popular tool for the analysis and visualization of flow cytometry data, including data related to erythroid cells.
The RNeasy Mini Kit is a common method for extracting and purifying RNA from erythroid cells, enabling further molecular analysis.
Understanding the biology and function of erythroid cells is crucial for the study and treatment of hematopoietic disorders, such as anemia, polycythemia, and various blood cancers.
Ongoing research in this field, facilitated by the use of advanced technologies and techniques, is essential for advancing our knowledge and improving healthcare outcomes.