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> Anatomy > Cell > Hepatic Stellate Cells

Hepatic Stellate Cells

Hepatic Stellate Cells are a type of stromal cell found in the liver.
These cells play a crucial role in liver fibrosis and regeneration.
They are responsible for the production and maintenance of the extracellular matrix, which provides structural support for the liver tissue.
Hepatic Stellate Cells can also transdifferentiate into myofibroblast-like cells, contributing to the development of liver cirrhosis and other chronic liver diseases.
Understainding the biology and function of these cells is essential for developing effective therapies to treat liver fibrosis and related disorders.

Most cited protocols related to «Hepatic Stellate Cells»

1
Detailed Liver Injury and Fibrosis Protocols
Alcoholic and hepatitis B-associated cirrhotic liver samples (stage 3-4 fibrosis) were collected from donor livers during liver transplantation from the Liver Tissue Cell Distribution System (LTCDS), University of Minnesota. The LTCDs were supported by NIH Contract #N01-DK-7-0004 / HHSN267200700004C. Additional information on the sample preparation, age and gender of the donors is provided in the Supplemental Materials.
The details of the induction of liver injury and fibrosis by CCl4 and bile duct ligation (BDL) and the treatment protocols are described in the Supplemental Methods.
The determination of liver function, histology and immunohistochemistry, quantitative analysis of hepatic fibrosis are described in the Supplemental Methods.
The determination of hepatic PARP and myeloperoxidase activities, 4-hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), and hydroxyproline contents, real-time PCR, Western immunoblot analysis are described in the Supplemental Methods.
Other procedures such as isolation and treatments of murine hepatic hepatocytes and stellate cells, cell death determination by flow cytometer and activation of hepatic stellate cells are also described in the Supplemental Methods.
Mukhopadhyay P., Rajesh M., Cao Z., Horváth B., Park O., Wang H., Erdelyi K., Holovac E., Wang Y., Liaudet L., Hamdaoui N., Lafdil F., Haskó G., Szabo C., Boulares A.H., Gao B, & Pacher P. (2014). Poly (ADP-ribose) polymerase-1 is a key mediator of liver inflammation and fibrosis. Hepatology (Baltimore, Md.), 59(5), 1998-2009.
Publication 2013
3-nitrotyrosine 4-hydroxy-2-nonenal Alcoholics CCL4 protein, human Cell Death Cells Cell Transplantation Determination of Death Donors Duct, Bile Fibrosis Fibrosis, Liver Hepatic Stellate Cells Hepatitis B Hepatocyte Hydroxyproline Immunohistochemistry Injuries isolation Ligation Liver Mus Peroxidase Portal System Real-Time Polymerase Chain Reaction Tissue Donors Tissues Tissue Transplantation Transplantation Treatment Protocols Western Blot
2
STING-mediated Macrophage-Hepatocyte Crosstalk

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Publication 2018
Bone Marrow Cells Cell Lines Cells Coculture Techniques Culture Media, Conditioned cyclic guanosine monophosphate-adenosine monophosphate Hepatic Stellate Cells Hepatocyte Homo sapiens Inflammation Macrophage Mus TGF-beta1 vadimezan
3
Cholangiocyte isolation and IMCL transfection
Cholangiocytes were obtained by immunoaffinity separation (4 (link)). The in vitro studies were performed in vector or Sct stable-transfected IMCL (4 (link)). The IMCL control (stable transfected with the empty vector) or IMCL lacking Sct was established using SureSilencing short hairpin RNA (Super-Array, Frederick, MD) plasmid for mouse Sct containing a marker for neomycin resistance for the selection of stably transfected cells. The human hepatic stellate cells (HHSteC) were purchased from Sciencell (Carlsbad, CA).
Wu N., Meng F., Invernizzi P., Bernuzzi F., Venter J., Standeford H., Onori P., Marzioni M., Alvaro D., Franchitto A., Gaudio E., Glaser S, & Alpini G. (2016). The secretin/secretin receptor axis modulates liver fibrosis through changes in TGF-β1 biliary secretion. Hepatology (Baltimore, Md.), 64(3), 865-879.
Publication 2016
Cells Cloning Vectors Hepatic Stellate Cells Homo sapiens Mus Neomycin Plasmids Short Hairpin RNA
4
Isolation and Culture of Hepatic Stellate Cells
C57BL/6 mice 8-12 week old were from Charles River. All animals received humane care according to the criteria outlined in the “Guide for the Care and Use of Laboratory Animals”. Hepatic stellate cells (HSC) were isolated from C57BL/6 mice by perfusion with collagenase-pronase as described (29 (link)) with small modifications. HSC were separated from parenchymal cells by 60×g centrifugation, collecting the supernatant for centrifugation at 450×g for 10min. Pellet or non-parenchymal cells were resuspended and purified over a 17.2% Hystodenz density gradient by centrifugation. The cloudy strip was collected and the HSC were cleaned with Krebs-Henseleit buffer by centrifugation of 450×g for 10 minutes. Cells were cultured in DMEM complemented with 10% FBS, and antibiotics at 37°C in a humidified atmosphere of 95% air and 5% CO2. Culture purity was assessed by retinoid autofluorescence. Mouse HSC were not passaged and were used from day-2 to day-10.
Moles A., Tarrats N., Fernández-Checa J.C, & Marí M. (2009). CATHEPSINS B AND D DRIVE HEPATIC STELLATE CELL PROLIFERATION AND PROMOTE THEIR FIBROGENIC POTENTIAL. Hepatology (Baltimore, Md.), 49(4), 1297-1307.
Publication 2009
Animals Animals, Laboratory Antibiotics Atmosphere Cells Centrifugation Collagenase Hepatic Stellate Cells Krebs-Henseleit solution Mice, Inbred C57BL Mus Perfusion Pronase Retinoids Rivers
5
Isolation and Immortalization of Mouse Hepatic Stellate Cells
Mouse hepatic stellate cells (mHSCs) were isolated from C57/Bl6 WT, TLR4−/−, and MyD88−/− mice by enzymatic digestion and Percoll density gradient centrifugation,24 (link) with modifications. Following two to three passages, 50% subconfluent mHSCs were transfected with pCMV Simian Virus 40 Large T antigen22 (link) for 48 hours followed by selection with 100 mg/mL hygromycin B (Invitrogen, San Diego, CA) and grown at 33°C. Immortalized WT, TLR4−/−, and MyD88−/− mHSC lines were generated by single cell clonal expansion and were genotyped as described for the TLR4 knockout mice.25 (link),26 (link)
Guo J., Loke J., Zheng F., Hong F., Yea S., Fukata M., Tarocchi M., Abar O.T., Huang H., Sninsky J.J, & Friedman S.L. (2009). Functional Linkage of Cirrhosis-Predictive Single Nucleotide Polymorphisms of Toll-like Receptor 4 to Hepatic Stellate Cell Responses. Hepatology (Baltimore, Md.), 49(3), 960-968.
Publication 2009
Centrifugation, Density Gradient Clone Cells Digestion Enzymes Hepatic Stellate Cells Hygromycin B Mice, Knockout Mus Percoll Simian virus 40

Most recents protocols related to «Hepatic Stellate Cells»

1
Differential Expression Analysis of Liver and Hepatic Stellate Cells

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Publication 2023
Genes Hepatic Stellate Cells Homo sapiens Liver Transplantations
2
Culturing and Transfecting Cell Lines
Mouse embryonic fibroblast cell line NIH3T3 cells were purchased from American Type Culture Collection. Human hepatic stellate cell line LX-2 cells were purchased from Merck Millipore. NIH3T3 cells, cardiac myofibroblasts, and activated HSCs were cultured at 37 °C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific) and 1% penicillin-streptomycin solution (Nacalai Tesque). LX-2 cells were cultured at 37 °C with 5% CO2 in DMEM supplemented with 2% FBS and 1% penicillin-streptomycin solution.
Lipofectamine RNAiMAX (Thermo Fisher Scientific) was used for siRNA transfection in NIH3T3 cells, LX-2 cells, cardiac myofibroblasts, and activated HSCs, as per the manufacturer’s instructions. For drebrin overexpression in NIH3T3 cells, Lipofectamine 2000 (Thermo Fisher Scientific) was used as per the manufacturer’s instructions.
Hironaka T., Takizawa N., Yamauchi Y., Horii Y, & Nakaya M. (2023). The well-developed actin cytoskeleton and Cthrc1 expression by actin-binding protein drebrin in myofibroblasts promote cardiac and hepatic fibrosis. The Journal of Biological Chemistry, 299(3), 102934.
Publication 2023
Cell Culture Techniques Cell Lines Cells Eagle Embryo Fibroblasts Heart Hepatic Stellate Cells Homo sapiens Lipofectamine lipofectamine 2000 Mus Myofibroblasts NIH 3T3 Cells Penicillins RNA, Small Interfering Stem Cells, Hematopoietic Streptomycin Transfection
3
Isolation and Stimulation of Kupffer Cells
Primary hepatic stellate cells, Kupffer cells and hepatocytes were isolated as previously described and expression of Clec4n was measured by real-time qPCR.25 (link) Kupffer cells were isolated from C57BL/6 and Clec4n–/– mice and cultured with RPMI 1640 (Thermo Fisher Scientific) containing 10% FBS for 4 hours. After 12 hours of starvation in RPMI1640 (without FBS), Kupffer cells were stimulated with M. restricta lysate (40 µg/mL) or PBS (control) for 8 hours. M. restricta lysate was prepared by washing centrifugation pellets of M. restricta with PBS twice, dissolving in 1 mL PBS and then disrupting using 0.5 mm glass beads in a MINI-BEADBEATER BIOSPEC PRODUCTS twice for 30 seconds with 3 minutes cooling on ice between runs. The concentration of M. restricta lysate was 0.15 mg/mL, measured by bicinchoninic acid assay.
Zeng S., Hartmann P., Park M., Duan Y., Lang S., Llorente C., Wang Y., Cabré N., Fouts D.E., Bacher P., Jung W.H., Stärkel P, & Schnabl B. (2023). Malassezia restricta promotes alcohol-induced liver injury. Hepatology Communications, 7(2), e0029.
Publication 2023
bicinchoninic acid Biological Assay Centrifugation Hepatic Stellate Cells Hepatocyte Kupffer Cells Mus Neoplasm Metastasis Pellets, Drug
4
Cell Culture Protocols for Liver Research

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Publication 2023
Cell Lines Cells Culture Media Endothelium Fetal Bovine Serum Glutamine Hepatic Stellate Cells Hepatocyte Mycoplasma Penicillins Sinusoidal Beds Stem Cells, Hematopoietic Streptomycin
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Comparative Adipose Cell Lipid Staining
Three adipose-containing cell lines were used to compare the staining effects of different ORO solutions: human osteosarcoma cell 143B, rat hepatic stellate cell HSC-T6, and human retinal epithelial cell APRE-19, all of which were purchased from the American Type Culture Collection (Manassas, USA). Cells were plated into a 48-well plate and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, Carlsbad, USA) supplemented with 10% foetal bovine serum (FBS, Gibco, Carlsbad, USA), 100 U/mL penicillin, and 100 µg/mL streptomycin (Gibco, Carlsbad, USA) with 5% CO2 at 37°C with 5% CO2. 143B cells were seeded with an initial cell density of 40% and cultured for 24 h after adhesion. HSC-T6 cells were seeded with an initial cell density of 15–20%. After cell adhesion, the medium was changed into DMEM complete medium containing 100 μmol/L sodium oleate and 10 μmol/L all-trans retinoic acid for 3–5 days. APRE-19 cells were seeded with an initial cell density of 20–30%. After cell adhesion, the medium was changed into DMEM/F12 complete medium containing 100 μmol/L sodium oleate for 48 hours. Primary adipocytes were extracted from fat tissue of 5-week old rat and cultured for three days by using a modified ceiling culture method [34 (link)], the slide with adherent adipocytes was put in 12-well plated and fixed for staining. The above cells will be used randomly for subsequent staining.
Du J., Zhao L., Kang Q., He Y, & Bi Y. (2023). An optimized method for Oil Red O staining with the salicylic acid ethanol solution. Adipocyte, 12(1), 2179334.
Publication 2023
Adipocytes Cell Adhesion Cells Culture Techniques Eagle Epithelial Cells Germ Cells Hepatic Stellate Cells Homo sapiens Osteosarcoma osteum Penicillins Retina Streptomycin Tissue, Adipose Tretinoin

Top products related to «Hepatic Stellate Cells»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Stellate cell medium by ScienCell
Sourced in United States
Stellate Cell Medium is a cell culture medium specifically formulated to support the growth and maintenance of stellate cells. It provides the necessary nutrients and supplements for the optimal culture of these specialized cells.
5
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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Dulbecco modified eagle medium (dmem) by Merck Group
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DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium formulated to support the growth and maintenance of various cell lines. It provides a balanced salt solution and essential nutrients required for cell proliferation. DMEM is suitable for use in a wide range of cell culture applications.
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Pronase by Roche
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Pronase is a broad-spectrum proteolytic enzyme derived from the bacterium Streptomyces griseus. It is commonly used in laboratory settings to digest and break down proteins in various applications.
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Lipofectamine 2000 by Thermo Fisher Scientific
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
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L glutamine by Thermo Fisher Scientific
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.

More about "Hepatic Stellate Cells"

Hepatic stellate cells (HSCs), also known as Ito cells or lipocytes, are a specialized type of stromal cell found in the liver.
These cells play a crucial role in liver fibrosis, regeneration, and other chronic liver diseases.
HSCs are responsible for the production and maintenance of the extracellular matrix, which provides structural support for the liver tissue.
In a healthy liver, HSCs are in a quiescent state, storing lipids and vitamin A.
However, in response to liver injury or disease, HSCs can become activated and transdifferentiate into myofibroblast-like cells.
The activation of HSCs is a key driver of liver fibrosis, as these cells produce excessive amounts of collagen and other extracellular matrix proteins, leading to the formation of scar tissue.
This can ultimately result in the development of liver cirrhosis and other chronic liver diseases.
Understanding the biology and function of HSCs is essential for developing effective therapies to treat liver fibrosis and related disorders.
Researchers often use cell culture models, such as those involving fetal bovine serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), penicillin/streptomycin, and specialized stellate cell media, to study the behavior and characteristics of these cells.
In addition, various techniques, such as the use of Pronase for cell isolation and Lipofectamine 2000 for transfection, are employed to manipulate and investigate HSCs in the laboratory.
By elucidating the mechanisms underlying HSC activation and function, scientists can develop targeted therapies to prevent or reverse liver fibrosis and improve patient outcomes.