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K562 Cells

K562 cells are a well-established human myelogenous leukemia cell line commonly used in biomedical research.
These cells are derived from a chronic myelogenous leukemia (CML) patient in blast crisis and exhibit characteristics of immature granulocytes.
K562 cells are highly versatile, serving as a model for studies related to hematopoiesis, signal transduction, and the evaluation of novel therapeutic agents.
Researchers can leverage the power of PubCompare.ai to optimize their K562 cell experiments, effortlessly locating the most accurate and reprodcible protocols from literature, preprints, and patents.
Utilizing AI-driven comparisons, scientists can identify the best protocols and products for their K562 cell research, ensuring maximum efficiency and accuracy.

Most cited protocols related to «K562 Cells»

1
Efficient CRISPR Editing in Cell Lines
K562 cells (American Type Culture Collection) were cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (FBS, HyClone), 1% penicillin/streptomycin. A pool of K562 cells stably expressing green fluorescent protein (GFP) was generated by transduction with the lentivirus construct pCCLsin.PPT.hPGK.GFP.pre (10 (link)). Because the lentiviral construct integrates randomly, the distribution of GFP expression levels is broad. This cell pool also includes cells that that were not transduced and do not express GFP at all. For transient transfection with CRISPR vectors, 1 × 106 K562 cells were resuspended in Nucleofector Solution V (Lonza) with 1 μg plasmid DNA, and electroporated in an Amaxa 2D Nucleofector using program T-016. In case of LBR editing, a clonal K562 line stably transformed with Cas9 was used.
Human retinal pigment epithelial (RPE) cells were cultured in a 1:1 mixture of Dulbecco's modified Eagle's medium (Gibco) with Nutrient F12 (Gibco) supplemented with 10% FBS (HyClone), 1% penicillin/streptomycin. CRISPR vectors were transfected with 5 μl Lipofectamine 2000 Reagent (Invitrogen) and 2.5 μg plasmid DNA in 250 μl antibiotic-free medium (Gibco).
Kc167 cells were cultured in Shields and Sang M3 Insect Medium (Sigma-Aldrich) with 0.25% Bacto Peptone (BD), 0.1% Yeast Extract (BD), 5% heat-inactivated FBS and 1% penicillin/streptomycin. Note that 1 × 106 cells were electroporated with 1 μg each of Cas9 and sgRNA expression plasmid using a BioRad Gene Pulser II (450 μF, 86 V).
Brinkman E.K., Chen T., Amendola M, & van Steensel B. (2014). Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Research, 42(22), e168.
Publication 2014
5-hydroxyethoxy-N-acetyltryptamine Antibiotics Bacto-peptone Cells Clone Cells Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats Epithelial Cells Genes Genetic Vectors Green Fluorescent Proteins Homo sapiens Insecta K562 Cells Lentivirus lipofectamine 2000 Nutrients Penicillins Plasmids Retinal Pigments Streptomycin Transients Yeast, Dried
2
CUT&RUN protocol for K562 cells
Human K562 cells were purchased from ATCC (Manassas, VA, Catalog #CCL-243). CUT&RUN was performed using a centrifugation-based protocol. Ten million cells were harvested by centrifugation (600 g, 3 min in a swinging bucket rotor) and washed in ice cold phosphate-buffered saline (PBS). Nuclei were isolated by hypotonic lysis in 1 ml NE1 (20 mM HEPES-KOH pH 7.9; 10 mM KCl; 1 mM MgCl2; 0.1% Triton X-100; 20% Glycerol) for 5 min on ice followed by centrifugation as above. (We have found that nucleases in some cells cause Mg++-dependent degradation of DNA, in which case 0.5 mM spermidine can be substituted for 1 mM MgCl2.) Nuclei were briefly washed in 1.5 ml Buffer 1 (20 mM HEPES pH 7.5; 150 mM NaCl; 2 mM EDTA; 0.5 mM Spermidine; 0.1% BSA) and then washed in 1.5 ml Buffer 2 (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 0.1% BSA). Nuclei were resuspended in 500 µl Buffer 2 and 10 µl antibody was added and incubated at 4°C for 2 hr. Nuclei were washed 3 x in 1 ml Buffer 2 to remove unbound antibody. Nuclei were resupended in 300 µl Buffer 2 and 5 µl pA-MN added and incubated at 4°C for 1 hr. Nuclei were washed 3 x in 0.5 ml Buffer 2 to remove unbound pA-MN. Tubes were placed in a metal block in ice-water and quickly mixed with 100 mM CaCl2 to a final concentration of 2 mM. The reaction was quenched by the addition of EDTA and EGTA to a final concentration of 10 mM and 20 mM respectively and 1 ng of mononucleosome-sized DNA fragments from Drosophila DNA added as a spike-in. Cleaved fragments were liberated into the supernatant by incubating the nuclei at 4°C for 1 hr, and nuclei were pelleted by centrifugation as above. DNA fragments were extracted from the supernatant and used for the construction of sequencing libraries. We have also adapted this protocol for use with magnetic beads (Appendix 3).
Skene P.J, & Henikoff S. (2017). An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites. eLife, 6, e21856.
Publication 2017
Buffers Cell Nucleus Cells Centrifugation Cold Temperature Drosophila Edetic Acid Egtazic Acid Glycerin HEPES Homo sapiens Immunoglobulins K562 Cells Magnesium Chloride Metals Phosphates Saline Solution Sodium Chloride Spermidine Triton X-100
3
Natural Killer Cell Lysis Assay Protocol
Browne et al. [1 (link)] analysed the correlation matrix for eight measures of immune system function of 72 females with breast cancer, recorded during investigation of the physiological consequences of a psychological intervention [3 (link),4 (link)]. Four 51Cr-release measures of natural killer cell lysis were obtained using effector (NK cell) to target cell (K562 human myeloid cell) ratios of 100:1, 50:1, 25:1 and 12.5:1. Following Browne et al. [1 (link)] we designate these measures by their effector to target (E:T) ratios, NK100, NK50, NK25 and NK12 respectively. Similarly, natural killer cell lysis measured in the presence of recombinant interferon gamma (rIFNγ) using E:T ratios of 50:1, 25:1, 12.5:1 and 6.25:1, are designated IFN50, IFN25, IFN12, and IFN6 respectively. Lower E:T ratios are used in the presence of rIFNγ because rIFNγ increases NK cells' ability to rupture target cells.
The correlations reported in Browne et al.'s [1 (link)] Table 1 indicate that the four NK measures correlate highly with one another (average r = 0.852), and that the four rIFNγ enhanced NK measures also correlate highly with one another (averaging 0.960). However, the low correlations between the sets of NK and rIFNγ measurements (averaging only .111) indicate that the two sets of measurements reflect relatively distinct aspects of natural killer cell functioning. Browne et al. [1 (link)] viewed this as justifying the use of an exploratory two-factor model (Figure 1) which, unfortunately, was significantly inconsistent with the data (χ2 = 103.59, degrees of freedom (df) = 13, and probability p < 10-15). The small but significant residual differences between the data correlations and the correlations implied by the two-factor model were dismissed by Browne et al.[1 (link)] as "negligible from a practical point of view". SEMNET discussion of this model prompted Hayduk to investigate whether some unrecognized measurement feature was producing the significant, even if seemingly slight, ill fit.
Andersen, Farrar, Golden-Kreutz, Kutz, MacCallum, Courtney & Glaser [3 (link)] provide a description of the reasonably standard procedures used to obtain the Browne et al. [1 (link)] data. Peripheral blood leukocytes (PBLs) were obtained from 60 mL of venous blood, counted so that a known number of PBLs could be suspended in medium and incubated with either additional medium or additional medium plus rIFNγ. K562 target cells (a human myeloid cell line sensitive to NK cell activity) were labelled with 51Cr and aliquoted with the effector cells (either the NK, or the rIFNγ activated NK cells) in the ratios reported above. The cell mixture was centrifuged to ensure cell surface contact, and incubated to provide an opportunity for the NK cells to bind and rupture the target cells, thereby releasing the radioactive target cell cytoplasm. Gamma radioactivity of the supernatant collected from a second centrifuging indicated the effectiveness of the NK or rIFNγ-activated-NK cells at lysing the target cells, with larger measurements corresponding to more effective NK cell activity.
Hayduk L.A., Pazderka-Robinson H., Cummings G.G., Levers M.J, & Beres M.A. (2005). Structural equation model testing and the quality of natural killer cell activity measurements. BMC Medical Research Methodology, 5, 1.
Publication 2005
BLOOD Breast Carcinoma Cell Lines Cells Cytoplasm Females Gamma Rays Homo sapiens Interferon Type II K562 Cells Leukocytes Myeloid Cells Natural Killer Cells Radioactivity System, Immune Veins
4
Mapping Chromatin-Derived Enhancers
We defined silent and active enhancers from ENCODE HeLa-S3, GM12878 and K562 broad peaks (Broad Institute, Bernstein), downloaded from the UCSC ENCODE repository, according to the co-existence of histone modifications H3K4me1, H3K27ac and H3K27me3. Active enhancers were defined as co-localized H3K4me1 and H3K27ac peaks with no H3K27me3 peak, while silent enhancers were considered loci with H3K4me1 and H3K27me3 peaks but no H3K27ac peak. Loci were filtered to be located distant to TSSs (500 bp) and exons (200 bp) of protein-coding genes, multi-exonic non-coding genes and mRNAs (from ENSEMBL, GENCODE (v10), RefSeq and UCSC, downloaded January 12, 2012), and other lncRNAs from a gene-centric set derived from literature40 as well as manually annotated sense-antisense pairs (coding-noncoding and noncoding-noncoding sense-antisense pairs) with 5' EST and cDNA support, and 5' ESTs with no locus protein-coding capacity. Transcriptional differences between active and silent enhancer sets were determined by comparing the average number of FANTOM5 CAGE tag 5’ ends from the same ENCODE cell lines (pooled triplicates) in a window +/− 300 bp around the H3K4me1 peak mid points.
The active enhancer sets of HeLa-S3, GM12878 and K562 cells were then centered on proximal (within 200bp) P300 (Stanford, Snyder) ENCODE binding site peaks (joint P300 and GATA1 (Yale) peaks for K562) to derive center positions. FANTOM5 CAGE data from the same ENCODE cell lines (pooled triplicates) were then overlaid these centered enhancer regions and the absence (0) and presence (1) of (one or more) CAGE tag 5’ ends in 10bp non-overlapping windows were determined and an average profile was calculated to assess the average bidirectional pattern of transcription at chromatin-derived enhancers.
Pooled CAGE data from all FANTOM5 libraries (described above) were further overlaid with these regions and a directionality score based on the aggregate of CAGE tags falling within +/− 300bp from the center positions were calculated to determine potential strand bias. For comparison, we repeated the same calculations for genomic regions +/− 300bp around TSSs of RefSeq protein coding genes. Directionality was calculated as (F − R) / (F + R), where F and R is the sum of CAGE tags aligned on the forward and reverse strand, respectively. Directionality close to −1 or 1 indicates a unidirectional behavior while 0 indicates perfectly balanced bidirectional transcription.
Positional cross correlations were calculated between reverse and forward CAGE tag 5’ ends at ChIP-seq derived active HeLa-S3 and GM12878 enhancer center positions (as determined by P300 peaks) +/−300 bp (max lag 300) to identify their most likely separation. Cross correlations were also calculated in 300 bp windows (max lag 150) flanking the enhancer centers between CAGE 5’ ends and ENCODE H2A.Z signals (from the same cell line) for HeLa-S3 and GM12878 as well as between CAGE 5’ ends and ENCODE GM12878 nucleosome MNase-seq 5’ ends (9 pooled replicates). In the latter analysis, correlations were made using reads on the same strand. Pooled, unique CAGE tags (in which only one CAGE tag per bp was counted) were considered in all correlation analyses and enhancers were weighed according to the aggregated signal before subsequent averaging over lags not to make any library or enhancer have an undue influence.
Andersson R., Gebhard C., Miguel-Escalada I., Hoof I., Bornholdt J., Boyd M., Chen Y., Zhao X., Schmidl C., Suzuki T., Ntini E., Arner E., Valen E., Li K., Schwarzfischer L., Glatz D., Raithel J., Lilje B., Rapin N., Bagger F.O., Jørgensen M., Andersen P.R., Bertin N., Rackham O., Burroughs A.M., Baillie J.K., Ishizu Y., Shimizu Y., Furuhata E., Maeda S., Negishi Y., Mungall C.J., Meehan T.F., Lassmann T., Itoh M., Kawaji H., Kondo N., Kawai J., Lennartsson A., Daub C.O., Heutink P., Hume D.A., Jensen T.H., Suzuki H., Hayashizaki Y., Müller F., Forrest A.R., Carninci P., Rehli M, & Sandelin A. (2014). An atlas of active enhancers across human cell types and tissues. Nature, 507(7493), 455-461.
Publication 2014
Binding Sites cDNA Library Cell Lines Chromatin Chromatin Immunoprecipitation Sequencing DNA, Complementary EP300 protein, human Exons Expressed Sequence Tags GATA1 protein, human Gene Products, Protein Genes Genome HeLa Cells Histone Code Joints K562 Cells Multiple Birth Offspring Nucleosomes Proteins RNA, Long Untranslated RNA, Messenger Toxic Shock Syndrome Transcription, Genetic
5
Pooled CRISPR Screen in K562 Cells
A previously designed 4 sgRNA/gene CRISPR-Cas9 library was used targeting 5′ ends of conserved exons with sgRNAs varying in length between 19 and 25 base-pairs14 . The library was generated first by infecting K562 cells with a SFFV-Cas9-BFP vector to create a stably expressed Cas9 cell line. We then infected the lentiviral genome-wide sgRNA library into approximately 120 million cells following the same protocol as the genome-wide shRNA library to maintain at least 1,000-fold representation in cells. Infected cells were selected with puromycin (0.7 μg/mL, Sigma) for 3 days. Percentage of mCherry positive cells was measured by flow cytometry (BD Accuri C6). Selected cells were spun out of selection and into normal RPMI 1640 media. At T0, 120 million cells were spun down (300g for 5 min). Cells were then split into two populations and grown for 14 days, maintaining logarithmic growth (500,000 cells/mL) each day. After 14 days of growth, cells were pelleted by centrifugation, and genomic DNA was extracted for all three time samples separately following Qiagen’s Blood Maxi Kit instructions. sgRNA encoding constructs were analyzed by deep sequencing.
Morgens D.W., Deans R.M., Li A, & Bassik M.C. (2016). Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes. Nature biotechnology, 34(6), 634-636.
Publication 2016
BLOOD Cell Lines Cells Centrifugation Cloning Vectors Clustered Regularly Interspaced Short Palindromic Repeats DNA Library Exons Flow Cytometry Gene Library Genome Genomic Library K562 Cells Population Group Puromycin Short Hairpin RNA Spleen Focus-Forming Virus

Most recents protocols related to «K562 Cells»

1
Enhancing siRNA Delivery with 3E10 Peptide
Not available on PMC !

Example 3

Materials and Methods

Labeled siRNA (via attachment to fluorescein amidite, FAM) (1 nmole), or siRNA complexed with 3E10 (0.75 mg), was mixed at room temperature for 5 minutes. 200,000 K562 cells were then added to the suspension of 3E10, or siRNA alone, in serum free media. Additional serum free media was added to a final volume of 500 ul. Following incubation with cells at 37° C. for 24 hrs, the cells were centrifuged and washed three times with PBS prior to analysis by flow cytometry.

Results

The results are illustrated in flow cytometry dot plots (FIG. 3A-3C). % uptake was quantified (FIG. 3D).

The results show increased cell uptake of siRNA when mixed with 3E10.

US11872286B2. Compositions and methods for delivery of nucleic acids to cells (2024-01-16). Yale University [US]. Inventors: Elias Quijano [US], Peter Glazer [US].
Patent 2024
Cells Culture Media, Serum-Free Flow Cytometry Fluorescein K562 Cells RNA, Small Interfering
2
Delivery of Labeled mRNA by 3E10 in K562 Cells
Not available on PMC !

Example 4

Materials and Methods

Labeled mRNA (by attachment to cyanine 5, Cy5) (2 ug) alone or labeled mRNA complexed with 3E10 (2.5, 5, and 10 uM), were mixed at room temperature for 5 minutes. The suspensions of 3E10 plus mRNA, or mRNA alone, were added to 200,000 K562 cells in serum free media. Additional serum free media was added to a final volume of 500 ul. Following incubation with cells at 37° C. for 24 hrs, the cells were centrifuged and washed three times with PBS prior to analysis by flow cytometry.

Results

The results are illustrated in flow cytometry dot plots (FIG. 4A-4H). % uptake was quantified (FIG. 4I).

The results show increased uptake of mRNA when mixed with 3E10. Note that delivery of mRNA by the D31N variant of 3E10 resulted in the highest levels of mRNA cell uptake.

Fluorescent microscopy showed functional GFP expression in U2OS cells after translation of the same Cy5 labeled mRNA, which encodes for a green fluorescent protein (GFP) reporter.

US11872286B2. Compositions and methods for delivery of nucleic acids to cells (2024-01-16). Yale University [US]. Inventors: Elias Quijano [US], Peter Glazer [US].
Patent 2024
Cells Culture Media, Serum-Free Flow Cytometry Green Fluorescent Proteins K562 Cells Microscopy Obstetric Delivery RNA, Messenger
3
Plasmid Uptake Efficiency with 3E10
Not available on PMC !

Example 6

Materials and Methods

GFP reporter plasmid DNA (250 ug) was complexed with 3E10 (10 uM) at room temperature for 5 minutes. The suspension of 3E10 plus plasmid DNA, or plasmid DNA alone, were added to 200,000 K562 cells in serum free media. Additional serum free media was added to a final volume of 500 ul. Following incubation with cells at 37° C. for 24 hrs, the cells were centrifuged and washed three times with PBS. 72 hours after the initial treatment, cells were imaged and analyzed for GFP expression.

Results

Results indicate that GFP plasmid was robustly taken up by cells when 3E10 was combined with the plasmid DNA, as measured by green fluorescence, indicating uptake and functional expression of the GFP construct. No uptake or green fluorescence was seen when plasmid DNA alone was used. (FIG. 6).

US11872286B2. Compositions and methods for delivery of nucleic acids to cells (2024-01-16). Yale University [US]. Inventors: Elias Quijano [US], Peter Glazer [US].
Patent 2024
Cells Culture Media, Serum-Free Fluorescence K562 Cells Plasmids Vision
4
Monitoring Signaling Pathways in CML Cell Lines
Not available on PMC !

Example 7

In order to provide a more readily available and reproducible cell system (and to avoid the problems seen with existing methods), experimental systems based on tissue culture cell lines may be utilized to monitor the impact of drugs on signaling pathways.

Flow cytometric methods using tissue culture cells have been routinely used for investigating the effects of drugs, for example, inhibitors of Bcr/Abl kinase that are useful in the therapy of chronic myeloid leukemia (CML). CML is associated with the Philadelphia chromosome, a genetic translocation that fuses the Abl1 gene on chromosome 9 with part of the BCR gene on chromosome 22. The resulting fusion protein contains a receptor tyrosine kinase that constitutively activates several downstream signaling pathways, including P-STAT5, P-Crkl, P-mTOR, and P—HSF. The Abl kinase is the target of several therapeutics currently used clinically, including imatinib (GLEEVEC™), nilotinib, and dasatinib. These compounds act by inhibiting the tyrosine kinase activity at the receptor level, and also concomitantly inhibit all downstream signaling pathways.

As a representative model of CML, human K562 cell line, which expresses the Bcr/Abl fusion protein and constitutively phosphorylates the downstream STAT5 target (Cytometry 54A; 75-88, 2003), was used in the following experiment. As shown in FIG. 10, treatment of K562 cells for 30 min with 2 μM GLEEVEC™ (imatinib, or STI571) results in >95% inhibition of the phosphorylation of the downstream STAT5 target. Also, as shown in FIG. 10, although the phosphorylation of STAT5 is inhibited after 30 min imatinib exposure, there is no change in the cell cycle, as measured by DNA content.

Phosphorylated STAT5 (P-STAT5) acts as a transcriptional activator of several target proteins, including Cyclin D. Constitutive expression of Cyclin D (induced by P-STAT5) maintains K562 cells in cell cycle. It was found that exposure to imatinib for 24 hr decreases S-phase (as a marker of cell proliferation) by ˜50%, and further exposure to imatinib for an additional 24 hr decreases S-phase by an additional 50-70% (data not shown).

US11867690B2. Identification of functional cell states (2024-01-09). ASEDASCIENCES AG [CH]. Inventors: Bartlomiej Rajwa [US], Vincent T Shankey [US].
Patent 2024
Cell Culture Techniques Cell Cycle Cell Lines Cell Proliferation Cells Chromosomes, Human, Pair 9 Chromosomes, Human, Pair 22 CRKL protein Cyclin D Cyclins Dasatinib Flow Cytometry FRAP1 protein, human Fusion Proteins, bcr-abl Genes Gleevec Homo sapiens Imatinib inhibitors K562 Cells Leukemias, Chronic Granulocytic nilotinib Pharmaceutical Preparations Philadelphia Chromosome Phosphorylation Phosphotransferases Proteins Protein Targeting, Cellular Psychological Inhibition Receptor Protein-Tyrosine Kinases SERPINA3 protein, human Signal Transduction Pathways Staphylococcal Protein A STAT5A protein, human STI571 Tissues Transcription, Genetic Translocation, Chromosomal Vision
5
Enhancing PNA Cellular Uptake with 3E10
Not available on PMC !

Example 1

Materials and Methods

PNA alone (1 nmole) (MW=9984.39; 29 nucleotides in length), or PNA complexed with 3E10 (0.75 mg), was mixed at room temperature for 5 minutes. 200,000 K562 cells were then added to the suspension of 3E10, or PNA alone, in serum free media. Additional serum free media was added to a final volume of 500 ul. Following incubation with cells at 37° C. for 1 hr, the cells were centrifuged and washed three times with PBS prior to analysis by flow cytometry. The PNA was labeled by attachment to the fluorescent dye, tetramethylrhodamine (TAMRA).

Results

The results are illustrated in flow cytometry dot plots (FIG. 1A-1C). % uptake was quantified (FIG. 1D).

The results show increased uptake of PNA when mixed with 3E10.

US11872286B2. Compositions and methods for delivery of nucleic acids to cells (2024-01-16). Yale University [US]. Inventors: Elias Quijano [US], Peter Glazer [US].
Patent 2024
Cells Culture Media, Serum-Free Flow Cytometry Fluorescent Dyes K562 Cells Nucleotides tetramethylrhodamine

Top products related to «K562 Cells»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Rpmi 1640 medium by Thermo Fisher Scientific
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
3
Rpmi 1640 by Thermo Fisher Scientific
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
4
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
5
K562 cell by American Type Culture Collection
Sourced in United States, United Kingdom
K562 cells are a human immortalized myelogenous leukemia cell line derived from a 53-year-old female patient. They are suspension cells and grow in single-cell or small cluster formation. K562 cells are a commonly used model for studying cellular processes in leukemia.
6
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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Lipofectamine 2000 by Thermo Fisher Scientific
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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.
8
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
10
L glutamine by Thermo Fisher Scientific
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.

More about "K562 Cells"

K562 cells are a well-established human myelogenous leukemia cell line that are widely used in biomedical research.
These cells are derived from a chronic myelogenous leukemia (CML) patient in blast crisis and exhibit characteristics of immature granulocytes.
K562 cells are highly versatile and serve as a valuable model for studies related to hematopoiesis, signal transduction, and the evaluation of novel therapeutic agents.
Researchers can leverage the power of PubCompare.ai to optimize their K562 cell experiments.
This AI-powered platform enables scientists to effortlessly locate the most accurate and reproducible protocols from literature, preprints, and patents.
By utilizing AI-driven comparisons, researchers can identify the best protocols and products for their K562 cell research, ensuring maximum efficiency and accuracy.
When working with K562 cells, it is important to consider the culture media and supplements.
Commonly used media include RPMI 1640 and DMEM, often supplemented with fetal bovine serum (FBS), L-glutamine, and antibiotics such as penicillin and streptomycin.
The choice of media and supplements can significantly impact the growth, differentiation, and experimental outcomes of K562 cells.
Furthermore, transfection reagents like Lipofectamine 2000 can be utilized to introduce plasmids, siRNA, or other genetic materials into K562 cells, enabling researchers to investigate gene expression, signaling pathways, and cellular responses.
By leveraging the insights and capabilities of PubCompare.ai, scientists can streamline their K562 cell research, optimizing protocols, identifying the best reagents, and ultimately enhancing the accuracy and reproducibility of their findings.
Expereincing the power of this AI-driven platform can be a game-changer for researchers working with K562 cells.