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> Anatomy > Cell > Keratinocyte

Keratinocyte

Keratinocytes are the predominant cell type in the epidermis, the outermost layer of the skin.
These cells play a critical role in maintaining the skin's barrier function and are involved in various processes, such as wound healing and immune response.
Keratinocytes undergo a complex differentiation process, transforming from basal cells to anucleate corneocytes that make up the stratum corneum.
Understanding the biology and regulation of keratinocytes is essential for advancing research in areas like skin diseases, regenerative medicine, and cosmetic science.
Puvcompare.ai offers powerful AI-driven tools to optimize your keratinocyte research, helping you discover the best protocols and products while enhancing reproducibility and accuracy.

Most cited protocols related to «Keratinocyte»

1
Comprehensive Head and Neck Cell Line Database
A total of 85 unique head and neck cell lines were used in our research, including 61 HNSCC cell lines, 11 thyroid cancer cell lines, 3 cutaneous SCC cell lines, 5 immortalized normal keratinocyte cell lines, 3 immortalized normal oral epithelial cell lines, and 2 leukoplakia cell lines. Information regarding each cell line and appropriate culture media is presented in Tables 1 and 2. The FaDu, CAL-27, Detroit562, SCC-4, SCC-9, SCC-15, and SCC-25 cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA). The OSC-19 cell line was obtained from the Health Science Research Resource Bank (Osaka, Japan). The B-CPAP cell line was obtained from the German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; Braunschweig, Germany). The NHEK cell line was purchased from Lonza Rockland, Inc., (Rockland, ME). The Nthy-ori 3-1 cell line was obtained from the European Collection of Cell Cultures (Wiltshire, United Kingdom). The sources for the other cell lines were as listed in Tables 1 and 2.
Adherent monolayer cultures were maintained on plastic and incubated at 37°C in 5% carbon dioxide and 95% air. To maintain the integrity of the collections, we carefully maintained the cell lines in culture and stored stocks of the early-passage cells. The cultures were free of Mycoplasma species and were maintained for no longer than 12 weeks after recovery from frozen stocks in culture.
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.E, & Myers J.N. (2011). Assembly And Initial Characterization Of A Panel Of 85 Genomically Validated Cell Lines From Diverse Head And Neck Tumor Sites. Clinical cancer research : an official journal of the American Association for Cancer Research, 17(23), 7248-7264.
Publication 2011
B-Lymphocytes Carbon dioxide Carcinoma, Thyroid Cell Culture Techniques Cell Lines Cells Continuous Positive Airway Pressure Culture Media Europeans Freezing Head Keratinocyte Leukoplakia Mycoplasma Neck Skin Squamous Cell Carcinoma of the Head and Neck
2
Identifying Overexpressed Genes in Tumors
In a previous study [16 (link)] we calculated using CCLE the number of cell lines that over-express each gene (twofold more than the peak of expression distribution). For generating the signatures we only use genes that have an overexpression rate of less than 5% (less than 32 cell lines of the 634 carcinoma cell lines). We use this stringent threshold to eliminate genes that tend to be overexpressed in tumors, regardless of the cellular composition. Of 18,988 genes analyzed, 9506 were identified as not being overexpressed in tumors. For signatures of cell types that may be the cell of origin of solid tumors, including epithelial cells, sebocytes, keratinocytes, hepatocytes, melanocytes, astrocytes, and neurons, we used all genes.
Aran D., Hu Z, & Butte A.J. (2017). xCell: digitally portraying the tissue cellular heterogeneity landscape. Genome Biology, 18, 220.
Full text: Click here
Publication 2017
Astrocytes Carcinoma Cell Lines Epithelial Cells Genes Genes, Neoplasm Hepatocyte Keratinocyte Melanocyte Neoplasms Neurons
3
Generating Mouse OSCC Cell Lines from Primary Tumors
Primary DMBA induced mouse OSCC were generated as described (26 (link)). Single cell suspensions of individual primary oral cavity tumors were made with Collagenase IA (Sigma-Aldrich) and cultured in IMDM/F12 (2:1) with 5% FCS, penicillin/streptomycin, 1% amphotericin, 5 ng/mL EGF (Millipore), 400 ng/mL hydrocortisone, and 5 μg/mL insulin. Sequential differential trypsinization was then used to clear fibroblast contamination. MOC1, 7, 10, 22 and 23 were derived from primary tumors in C57BL/6 WT mice and MOC2 was derived from a chemokine receptor CXCR3deficient mouse on a pure C57BL/6 background (27 (link)) (of note, no major differences in the incidence of tumor formation were noted between the different genotypes). CXCR3 is not detectable on oral keratinocytes and does not contribute to MOC2 growth (Figure S3). Immunofluorescence staining for cytokeratin was performed to confirm an epithelial phenotype (Figure 1C and Figure S1C). PCI-13 was obtained from Dr. Theresa Whiteside, UPCI:SCC029B and UPCI:SCC068 were obtained from Dr. Suzanne Gollin, and all were used with minimal passaging. The UM-SCC-1 cell line (from Dr. Tom Carey) was genotyped in May, 2011 and concordance with published data was established (27 (link)).
Judd N.P., Winkler A.E., Murillo-Sauca O., Brotman J.J., Law J.H., Lewis JS J.r., Dunn G.P., Bui J.D., Sunwoo J.B, & Uppaluri R. (2011). ERK1/2 regulation of CD44 modulates oral cancer aggressiveness. Cancer Research, 72(1), 365-374.
Publication 2011
9,10-Dimethyl-1,2-benzanthracene Amphotericin Cell Lines Cells Chemokine Receptor Collagenase CXCR3 protein, human Cytokeratin Fibroblasts Hydrocortisone Immunofluorescence Insulin Keratinocyte Mice, Inbred C57BL Mouth Neoplasms Mus Neoplasms Penicillins Phenotype Streptomycin
4
Comprehensive Head and Neck Cancer Cell Panel
A panel of 122 human head and neck cell lines was assembled from a number of different researchers, institutions, and suppliers. This panel was chosen to represent each of the major HNSCC sites: oral cavity, oropharynx, hypopharynx, and larynx. Also chosen for study were anaplastic and papillary thyroid cancer, adenoid cystic carcinoma cell lines, and cell lines derived from lymph node metastases. In some cases isogenic cell line pairs were obtained, which included cells derived from both the primary tumor and lymph node metastases from the same patient. Also included were cell lines from cutaneous SCC, leukoplakia, immortalized primary keratinocytes, and normal epithelium.
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.E, & Myers J.N. (2011). Assembly And Initial Characterization Of A Panel Of 85 Genomically Validated Cell Lines From Diverse Head And Neck Tumor Sites. Clinical cancer research : an official journal of the American Association for Cancer Research, 17(23), 7248-7264.
Publication 2011
Adenoid Cystic Carcinoma Anaplasia Cell Lines Cells Epithelium Head Homo sapiens Hypopharynx Keratinocyte Larynx Leukoplakia Lymph Node Metastasis Neck Neoplasms Oral Cavity Oropharynxs Papillary Thyroid Carcinoma Patients Skin Squamous Cell Carcinoma of the Head and Neck
5
Multistep Skin Carcinogenesis Assays
Multistep skin carcinogenesis assays were performed with mice with keratinocyte-specific deletion of the CnB1 gene (CnB1loxP/loxPxK5-CrePR1)3 (link) in parallel with Cre-negative controls (CnB1loxP/loxP). Keratinocyte grafting assays were performed as previously described29 (link). Intradermal tumourigenicity assays were adapted from hair follicle reconstitution assays30 (link). Detailed conditions for these assays as well as chromatin immunoprecipitation, immunoblotting, immunofluorescence, senescence β-galactosidase staining, biotinylated DNA pull down assays, and sorting can be found in the Method section and supplementary figure legends.
Wu X., Nguyen B.C., Dziunycz P., Chang S., Brooks Y., Lefort K., Hofbauer G.F, & Dotto G.P. (2010). Calcineurin and ATF3: opposite roles in squamous skin cancer. Nature, 465(7296), 368-372.
Publication 2010
Biological Assay Carcinogenesis Gene Deletion GLB1 protein, human Hair Follicle Immunofluorescence Immunoprecipitation, Chromatin Keratinocyte Mice, House Skin

Most recents protocols related to «Keratinocyte»

1
Inhibition of AhR nuclear translocation
Not available on PMC !

Example 2

HaCaT cells, human keratinocyte cells, were seeded on a 6-well plate at a density of 3×105 cells/well and cultured overnight. Subsequently, 10 nM of TCDD and 50 μM of the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 were added to the culture medium. After 30 minutes of reaction, the cells were treated for 1 hour and collected to obtain nuclei and cytoplasmic proteins separated from each other. Westin blotting was performed using an aryl hydrocarbon receptor (AhR) antibody (Santa Cruz Biotechnology, U.S.A.) to identify activated nuclear translocation of AhR, and the results are shown in FIGS. 2A to 2F and Table 3.

TABLE 3
TCDD + Peptide
SEQ ID NO:ControlTCDD5 μM50 μM
11 times5.8 times1.9 times1.9 times
21 times5.7 times2.5 times0.9 times
31 times5.9 times  3 times1.5 times

As shown in FIGS. 2A to 2F and Table 3, it was confirmed that the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 inhibits nuclear translocation of AhR by TCDD.

US11859016B2. Peptide having cytoprotective effect against environmental pollutant and use thereof (2024-01-02). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung-ji Lee [KR].
Full text: Click here
Patent 2024
AHR protein, human Amino Acid Sequence Cardiac Arrest Cell Nucleus Cells Culture Media Cytoplasm Figs Homo sapiens Immunoglobulins Keratinocyte Peptides Proteins Tetrachlorodibenzodioxin Translocation, Chromosomal
2
Peptide Inhibits Particulate Matter-Induced AhR Translocation
Not available on PMC !

Example 6

HaCaT cells, human keratinocyte cells, were seeded on a 6-well plate at a density of 3×105 cells/well and cultured overnight. 10 nM of urban particulate matter (PM, Sigma Aldrich, USA) and 50 μM of the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 were added to the culture medium. After 30 minutes of reaction, the cells were treated for 1 hour and collected to obtain nuclei and cytoplasmic proteins separated from each other. Then, Westin blotting was performed using aryl hydrocarbon receptor (AhR) antibody (Santa Cruz Biotechnology, USA) to identify activated nuclear translocation of AhR, and the results are shown in FIGS. 6A to 6C.

As shown in FIGS. 6A to 6C, the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 inhibited nuclear translocation of AhR by particulate matter.

US11859016B2. Peptide having cytoprotective effect against environmental pollutant and use thereof (2024-01-02). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung-ji Lee [KR].
Full text: Click here
Patent 2024
Amino Acid Sequence Aryl Hydrocarbon Receptor Cell Nucleus Cells Culture Media Cytoplasm Figs Homo sapiens Immunoglobulins Keratinocyte Peptides Proteins Translocation, Chromosomal
3
Peptide Reduces TCDD-Induced ROS in HaCaT Cells
Not available on PMC !

Example 4

HaCaT cells, human keratinocyte cells, were seeded on a 6-well plate at a density of 3×105 cells/well and cultured overnight. Subsequently, 10 nM of TCDD and 50 μM of the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 were added to the culture medium. After 30 minutes of reaction, the cells were treated for 24 hours and further treated with DCFH-DA for 30 minutes. Then, the cells were collected and subjected to FACS analysis to observe changes of average FL1 values, and the results are shown in FIGS. 4A to 4C.

As shown in FIGS. 4A to 4C, the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 reduced ROS levels increased by TCDD in cells.

US11859016B2. Peptide having cytoprotective effect against environmental pollutant and use thereof (2024-01-02). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung-ji Lee [KR].
Full text: Click here
Patent 2024
Amino Acid Sequence Cells Culture Media diacetyldichlorofluorescein Figs HaCaT Cells Homo sapiens Keratinocyte Peptides Tetrachlorodibenzodioxin
4
Optimizing Morphogen Exposure in Stem Cell Differentiation
Not available on PMC !

Example 6

Optimization of Morphogen Exposure

The optimal duration of caudalization and ventralization may vary depending on the parent cell line used, culture conditions, and quality of reagents. For cells with ESC origin both caudalization and ventralization are typically 1 day faster, for hiPSC derived from adult cells, the time can depend on the origin of the somatic cells. Several different types of cells have been used to produce iPSCs, including fibroblasts, neural progenitor cells, keratinocytes, melanocytes, CD34+ cells, hepatocytes, cord blood cells and adipose stem cells. In hiPSC derived from CD34+ cells caudalization and ventralization may be slower for up to 2 days. hiPSC derived from fibroblasts typically follow the time line as explained in the FIG. 1.

US11859206B2. Generation of oligodendrogenic neural progenitor cells (2024-01-02). UNIVERSITY HEALTH NETWORK [CA]. Inventors: Michael George Fehlings [CA], Mohamad Khazaei [CA].
Full text: Click here
Patent 2024
Adipocytes Adult Blood Cells Cell Lines Cells Cone-Rod Dystrophy 2 Fibroblasts Gene Therapy, Somatic Germ Cells Hepatocyte Human Induced Pluripotent Stem Cells Induced Pluripotent Stem Cells Keratinocyte Melanocyte Neural Stem Cells Neurogenesis Parent Stem, Plant TimeLine Umbilical Cord Blood
5
Inhibition of TCDD Cell Uptake by Peptides
Not available on PMC !

Example 3

HaCaT cells, human keratinocyte cells, were seeded on a 6-well plate at a density of 3×105 cells/well and cultured overnight. Subsequently, 50 nM of TCDD and 50 μM of the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 were added to the culture medium. After 30 minutes of reaction, the cells were treated for 5 minutes and immobilized with 4% paraformaldehyde for 30 minutes. Then, after washing three times, the cells were reacted with 0.5% Triton X-100 for 15 minutes and washed three times. Subsequently, the cells were blocked with 3% BSA for 1 hour and reacted with a primary antibody against TCDD conjugated with fluorescein isothiocyanate (FITC) (1:100) at 4° C. overnight. The cells were stained and mounted with 4,6-diamidino-2-phenylindole (DAPI) and observed with a fluorescence microscope. The results are shown in FIGS. 3A to 3C.

As shown in FIGS. 3A to 3C, the peptide consisting of an amino acid sequence of SEQ ID NO: 1, 2, or 3 inhibited introduction of TCDD into cells.

US11859016B2. Peptide having cytoprotective effect against environmental pollutant and use thereof (2024-01-02). CAREGEN CO., LTD. [KR]. Inventors: Yong Ji Chung [KR], Eun Mi Kim [KR], Eung-ji Lee [KR].
Full text: Click here
Patent 2024
Amino Acid Sequence Cells Culture Media Figs Fluorescein HaCaT Cells Homo sapiens Immunoglobulins isothiocyanate Keratinocyte Microscopy, Fluorescence paraform Peptides Tetrachlorodibenzodioxin Triton X-100

Top products related to «Keratinocyte»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Keratinocyte serum free medium by Thermo Fisher Scientific
Sourced in United States, United Kingdom, China, Germany, Canada, Italy, Switzerland
Keratinocyte serum-free medium is a specialized cell culture medium designed to support the growth and maintenance of keratinocyte cells in an in vitro environment. It provides the necessary nutrients and growth factors required for the proliferation and differentiation of keratinocytes without the need for serum supplementation.
5
Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
6
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
7
Bovine pituitary extract by Thermo Fisher Scientific
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Bovine pituitary extract is a cell culture supplement derived from the pituitary glands of bovine (cattle) origin. It is commonly used as a growth-promoting additive in cell culture media to support the proliferation and survival of various cell types.
8
Rpmi 1640 medium by Thermo Fisher Scientific
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
9
Epidermal growth factor (egf) by Thermo Fisher Scientific
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EGF is a lab equipment product from Thermo Fisher Scientific. It is a recombinant human Epidermal Growth Factor (EGF) protein. EGF is a growth factor that plays a role in cell proliferation and differentiation.
10
Rpmi 1640 by Thermo Fisher Scientific
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.

More about "Keratinocyte"

Keratinocytes are the predominant cell type in the epidermis, the outermost layer of the skin.
These vital skin cells play a critical role in maintaining the skin's protective barrier and are involved in various essential processes, such as wound healing and immune response.
The keratinocyte differentiation process is complex, transforming from basal cells to anucleate corneocytes that make up the stratum corneum, the skin's outermost layer.
Understanding the biology and regulation of keratinocytes is crucial for advancing research in areas like skin diseases, regenerative medicine, and cosmetic science.
Researchers often culture keratinocytes in vitro using specialized media, such as keratinocyte serum-free medium (KSFM), which is supplemented with growth factors like epidermal growth factor (EGF) and bovine pituitary extract.
Other common cell culture components include fetal bovine serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), and antibiotics like penicillin and streptomycin.
Optimizing keratinocyte research can be greatly enhanced by utilizing AI-driven tools like PubCompare.ai.
These powerful platforms enable researchers to discover the best protocols and products by leveraging comparisons across the scientific literature, preprints, and patents.
By enhancing reproducibility and accuracy, researchers can accelerate their investigations into the complex biology of these essential skin cells and their applications in fields like dermatology, skin regeneration, and cosmetic formulations.