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L Cells

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Most cited protocols related to «L Cells»

Nicotinic Receptor Purification and Characterization

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Publication 2016

Cells Centrifugation cholesterol-hemisuccinate Crystallization Endoglycosidases Enzymes G-800 Gel Chromatography L Cells Mass Spectrometry Nicotine Proteins SDS-PAGE Sodium Butyrate Sodium Chloride Streptococcal Infections Tissue, Membrane Tromethamine Trypsin Virus

Wnt Signaling Reporter Assays

Reporter assays have been described earlier [30] (link). While LS/L cells [24] (link) had been stably transfected with pSuperTOPFlash and pEF1/Myc-His/LacZ (Invitrogen), HEK293A and HEK293T cells were transfected with pMegaTOPFlash [28] (link) and pEF1/Myc-His/LacZ or pEF1α-LacZ-IRES-Puro^R one day prior to the addition of Wnt3A. Reporter assays were performed with the dual-light combined reporter gene assay system (Applied Biosystems) with a Centro LB960 luminometer (Berthold) according to the manufacturer's instructions. β-galactosidase activity was used to normalize the values, which are represented with their standard deviation.

Fuerer C, & Nusse R. (2010). Lentiviral Vectors to Probe and Manipulate the Wnt Signaling Pathway. PLoS ONE, 5(2), e9370.

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Publication 2010

beta-Galactosidase Biological Assay Cells Genes, Reporter Internal Ribosome Entry Sites LacZ Genes L Cells Light

In Vitro Cultivation and Genetic Manipulation of Malaria Parasite P. falciparum

Asexual blood-stage cultures of P. falciparum clone 3D7 were maintained in vitro and synchronized according to standard procedures (Blackman, 1994 (link); Yeoh et al., 2007 (link)) in RPMI 1640 medium containing the serum substitute Albumax (Invitrogen). For introduction of transfection constructs, mature schizonts were enriched from highly synchronous cultures using Percoll (GE Healthcare) as described previously (Harris et al., 2005 (link)), and transfected by electroporation with 10 μg of circular plasmid DNA using the Amaxa 4D electroporator (Lonza) and the P3 Primary cell 4D Nucleofector X Kit L (Lonza) and program FP158, exactly as recently described for P. knowlesi (Moon et al., 2013 (link)). Selection for parasites harbouring the plasmid was performed by culture in medium containing 2.5 nM WR99210. Selection for parasites in which integration of transfected DNA into the genome had taken place was promoted by cycles of culture in the absence and presence of WR99210, as described previously (Harris et al., 2005 (link)). When integration was detected by diagnostic PCR, integrant clones were obtained by limiting dilution and maintained in medium containing WR99210. Parasite growth rates were assessed by microscopic examination of Giemsa-stained thin films at 2-day intervals, and expressed as percentage parasitaemia (percentage of parasitized erythrocytes in the population)

Collins C.R., Das S., Wong E.H., Andenmatten N., Stallmach R., Hackett F., Herman J.P., Müller S., Meissner M, & Blackman M.J. (2013). Robust inducible Cre recombinase activity in the human malaria parasite Plasmodium falciparum enables efficient gene deletion within a single asexual erythrocytic growth cycle. Molecular Microbiology, 88(4), 687-701.

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Publication 2013

BLOOD Clone Cells Diagnosis DNA, Circular Electroporation Erythrocytes Genome L Cells Microscopy Parasitemia Parasites Percoll Plasmids Schizonts Serum Stain, Giemsa Technique, Dilution Transfection

Purification and Labeling of Reovirus Strains

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Publication 2007

bacteriophage T7 RNA polymerase Centrifugation cesium chloride Chick Embryo Fibroblasts HeLa Cells L Cells Methionine Reoviridae Strains Vaccinia virus Virion Virus

Efficient Genetic Manipulation in Leishmania

Unless otherwise stated, cells were transfected in 1× Tb-BSF buffer [30 (link)] using one pulse with program X-001 in the Amaxa Nucleofector IIb (Lonza).
For calculation of recombination rate, 10⁷L. mex Cas9 cells were transfected with 10⁵ molecules of respective DNA fragment per cell and 40 µg in vitro transcribed RNA. Cells were recovered in M199 for 8 h, then blasticidin was added and cells were plated on a 96-well plate. The mean rate of resistance from three independent transfections was calculated from the positive wells on plates seeded with 5 × 10⁶ cells and 5 × 10⁵ cells.
For double tagging of PF16, 10⁷L. mex Cas9 cells were co-transfected with 3 µg of donor DNA and 30 µg in vitro transcribed sgRNA for single tags or with 5 µg of each donor DNA and 100 µg sgRNA for double tagging.
For tests of in vivo sgRNA transcription, L. mex Cas9 T7 cells were transfected with 4 µg donor DNA and 4 µg sgRNA template DNA. Transfections with the Amaxa Nucleofector IIb used 3 × 10⁶ cells. For transfections with the BTX ECM 830 square wave electroporation system, 10⁷ cells were pulsed three times with 1.5 kV (unipolar, 100 µs, 10s interval) in 0.4 cm gap Gene Pulser electroporation cuvettes (BioRad).
For gene tagging with pPLOT constructs, 3 × 10⁶ cells were transfected with the PCR reactions for the sgRNA and donor DNA (combined volume approx. 50 µl) in a total volume of 250 µl. For gene KOs with pT constructs, 10⁷ cells were transfected with the PCR reactions for the two sgRNAs and two donor DNAs (combined volume approx. 100 µl) in a total volume of 250 µl.
For T. brucei gene tagging, PCR products were ethanol precipitated, DNA pellets resuspended in a total volume of 100 µl Tb-BSF buffer and mixed with 10⁷ cells in 100 µl Tb-BSF buffer.
Electroporated cells were immediately transferred into pre-warmed medium and left to recover for 4–16 h before adding selection drugs. Survival of drug-resistant transfectants typically became apparent 5–7 days after transfection.

Beneke T., Madden R., Makin L., Valli J., Sunter J, & Gluenz E. (2017). A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids. Royal Society Open Science, 4(5), 170095.

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Publication 2017

Buffers Cells Electroporation Ethanol Genes Interleukin-4 L Cells Pellets, Drug Pharmaceutical Preparations Recombination, Genetic Tissue Donors Transcription, Genetic Transfection

Most recents protocols related to «L Cells»

Preparation of Cell-free Lactobacillus Fractions

Not available on PMC !

Example 1

Cell-free fractions were prepared as previously described (25). Briefly, Lactobacillus acidophilus strain La-5 was grown overnight in modified DeMann, Rogosa and Sharpe medium. (mMRS; 10 g peptone from casein, 8 g meat extract, 4 g yeast extract, 8 g D(+)-glucose, 2 g dipotassium hydrogen phosphate, 2 g di-ammonium hydrogen citrate, 5 g sodium acetate, 0.2 g magnesium sulfate, 0.04 g manganese sulfate in 1 L distilled water) (MRS; BD Diagnostic Systems, Sparks, MD). The overnight culture was diluted 1:100 in fresh medium. When the culture grew to an optical density at 600 nm (OD₆₀₀) of 1.6 (1.2×10⁸cells/ml), the cells were harvested by centrifugation at 6,000×g for 10 min at 4° C. The supernatant was sterilized by filtering through a 0.2-μm-pore-size filter (Millipore, Bioscience Division, Mississauga, ON, Canada) and will be referred to as cell-free spent medium (CFSM). Two litres of L. acidophilus La-5 CFSM was collected and freeze-dried (Unitop 600 SL, VirTis Co., Inc. Gardiner, NY., USA). The freeze-dried CFSM was reconstituted with 200 ml of 18-Ω water. The total protein content of the reconstituted CFSM was quantified using the BioRad DC protein assay kit II (Bio-Rad Laboratories Ltd., Mississauga, ON, Canada). Freeze-dried CFSM was stored at −20° C. prior to the assays.

US11857581B2. Antiviral methods and compositions comprising probiotic bacterial molecules (2024-01-02). MicroSintesis Inc. [CA]. Inventors: Mansel Griffiths [CA].

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Patent 2024

ammonium citrate Biological Assay casein peptone Cells Centrifugation Diagnosis Freezing Glucose Hydrogen Lactobacillus acidophilus L Cells manganese sulfate Meat potassium phosphate, dibasic Proteins Sodium Acetate Sulfate, Magnesium Unitop Yeast, Dried

Quantitative ER-Mitochondria Interaction Assay

Mouse monoclonal anti ACTIN, Sigma-Aldrich, A5441; Mouse monoclonal anti c-myc, Sigma-Aldrich, M4439; Rabbit polyclonal anti Calnexin, Enzo, ADI-SPA-865-F; Mouse monoclonal anti CYTC, BD Bioscience, 556433; Rabbit DyLight 680, Thermo Fisher Scientific, 35569; Mouse DyLight 680 Thermo Fisher Scientific, 35519; Mouse DyLight 800 Thermo Fisher Scientific, 35521; Rabbit DyLight 800 Thermo Fisher Scientific, 35571; Mouse monoclonal anti eIF2α, Cell Signaling, 2103S; Rabbit polyclonal anti-E-Syt1, Sigma-Aldrich, HPA016858; Rabbit polyclonal anti-GFP, Cell Signaling, 2555S; Mouse monoclonal anti-GFP,Life technologies, A11122; HRP Mouse Bioké, Cell Signaling, 7076; HRP Rabbit Bioké, Cell Signaling, 7074; Mouse monoclonal anti IP3R3, BD Bioscience, 610312; Rabbit polyclonal anti-PERK, Cell signaling, 3192S; Rabbit polyclonal anti PERK, Cell signaling, 5683S; Rabbit monoclonal anti Phospho-eIF2α (Ser51), Cell signaling, 3597S; Rabbit polyclonal anti PDI Genetex, GTX30716; Mouse monoclonal anti PSD, Santa Cruz, sc-390070; Rabbit polyclonal anti, PSS1 (B-5), Santa Cruz, sc-515376; Rabbit polyclonal anti PSS2, Sigma-Aldrich, SAB1303408; Rabbit polyclonal VDAC1, Cell Signaling, 4866S; Rabbit polyclonal VDAC1, Abcam, ab15895; Veriblot antibody Abcam, ab131366.
The reagents used were: Antimycin A, Sigma-Aldrich, A8674; Calcium Chloride dihydrate, Sigma-Aldrich, C3881; CHAPS hydrate, Sigma-Aldrich, C3023; Conjugated GFP antibody beads, Laboratory of Chris Ulens; D-Galactose, Sigma-Aldrich, G0750; D-glucose, Sigma-Aldrich, G7021-1KG; DAPI, Thermo Fisher Scientific, 62248; Dulbecco’s Modified Eagle’s Medium - high glucose, Sigma-Aldrich, D0422; EGTA, AppliChem, A0878; FCCP, Sigma-Aldrich, C2920; Gibco DMEM/F-12, Thermo Fisher Scientific, 11320074; Glucose, Agilent Seahorse, 103577; Glutamine, Sigma-Aldrich, G7513; Glutamine, Agilent Seahorse, 103579; GSK PERK Inhibitor, Toronto Research Company, G797800; Hygromycin B, Invivogen, ant-hg-1; Lipofectamine 2000 Transfection Reagent, Thermo Fisher Scientific, 11668019; MitoTracker FarRed, Thermo Fisher Scientific, M22426; NBD-PS, Avanti Polar Lipids, 810194C; SE Cell Line 4D-Nucleofector X Kit L, V4XC-1024; Oligomycin, Sigma-Aldrich, 75351; Penicillin and streptomycin, Sigma-Aldrich, P0781; Percoll, Sigma-Aldrich, P1644; Pierce ECL Western Blotting Substrate, Thermo Fisher Scientific, 32106X4; Pierce Protein A/G Magnetic Beads, Thermo Fisher Scientific, 88802; Pierce Protease Inhibitor Tablets, EDTA-free, Thermo Fisher Scientific, 88266; Potassium Chloride, Janssen Chimica, 7447407; Protease inhibitor, Thermo Fisher Scientific, A32953; Puromycin, Thermo Fisher Scientific, A11138-03; Protein A/G PLUS-Agarose, Santa Cruz, sc-2003; XF DMEM pH7 7.4, Agilent Seahorse, 103575; Sodium Chloride, Sigma-Aldrich, A0431796; Sodium Pyruvate Solution, Agilent Seahorse, 103578; Sucrose, Acros, A0333146; Thapsigargin, Enzo Life Sciences, BML-PE180; TransIT-X2 Dynamic Delivery System, Mirus Bio, MIR 6000; Tris base, Sigma-Aldrich, 77861; Triton, Sigma-Aldrich, T9234; Tween, Sigma Aldrich, P4780.

Sassano M.L., van Vliet A.R., Vervoort E., Van Eygen S., Van den Haute C., Pavie B., Roels J., Swinnen J.V., Spinazzi M., Moens L., Casteels K., Meyts I., Pinton P., Marchi S., Rochin L., Giordano F., Felipe-Abrio B, & Agostinis P. (2023). PERK recruits E-Syt1 at ER–mitochondria contacts for mitochondrial lipid transport and respiration. The Journal of Cell Biology, 222(3), e202206008.

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Publication 2023

3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate Actins Antimycin A Calcium Chloride Dihydrate Calnexin Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone DAPI Eagle Edetic Acid Egtazic Acid G-substrate Galactose Glucose Glutamine Hygromycin B Immunoglobulins L Cells Lipids lipofectamine 2000 Mus N-(7-nitro-2,1,3-benzoxadiazol-4-yl)phosphatidylserine Obstetric Delivery Oligomycins Peeling Skin Syndrome Peeling skin syndrome, acral type Penicillins Percoll Potassium Chloride Protease Inhibitors Puromycin Pyruvate Rabbits Seahorses Sepharose Sodium Sodium Chloride Staphylococcal Protein A Streptomycin Sucrose SYT1 protein, human Thapsigargin Transfection Tromethamine Tweens VDAC1 protein, human

RNA Extraction and Sequencing of L. paraplantarum RX-8

Cell samples of L. paraplantarum RX-8 were obtained in co-culture and mono-culture at 24 h by centrifugation (10,000 rpm, 10 min, 4°C). After removing the supernatant, cells were immediately cleaned by phosphate buffered saline (PBS) two times, frozen in liquid nitrogen for 15 min, and then stored at −80°C.
Total RNA extractions were performed using Magen HiPure Universal RNA Kit (Magen, China). RNA concentration and purity were measured using Qubit 3.0 (Thermo Fisher Scientific, MA, USA) and Nanodrop One (Thermo Fisher Scientific, MA, USA), and integrity was confirmed using the Agilent 4200 system (Agilent Technologies, Waldbron, Germany). The RNA samples with an RNA integrity number (RIN) >6.5, and 280/260 ratios >1.5 were further used for RNA-sequencing purposes. Libraries were generated from three replicates using NEB Next^®Ultra™ Directional RNA Library Prep Kit for Illumina^® (New England Biolabs, MA, USA). From these libraries, the PE150 reads were produced with the Illumina Novaseq6000 platform by Guangdong Magigene Biotechnology Co., Ltd. (Guangzhou, China). All raw RNA-Seq data were submitted to NCBI under BioProject PRJNA901346.

Nie R., Zhu Z., Qi Y., Wang Z., Sun H, & Liu G. (2023). Bacteriocin production enhancing mechanism of Lactiplantibacillus paraplantarum RX-8 response to Wickerhamomyces anomalus Y-5 by transcriptomic and proteomic analyses. Frontiers in Microbiology, 14, 1111516.

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Publication 2023

cDNA Library Cells Centrifugation Coculture Techniques Freezing L Cells Nitrogen Phosphates RNA-Seq Saline Solution

Plantaricin and AI-2 Synthesis Genes Expression

To determine the expression level of plantaricin biosynthesis gene clusters (plnABCDEF) and AI-2 synthesis-related genes (pfs and luxS), cells of L. paraplantarum RX-8 were harvested through centrifugation (10,000 rpm, 2 min, 4°C) at every 4 h in mono-culture/co-culture (4–32 h). RNA was extracted from cells using the RNAprep Pure Cell/Bacteria Kit according to the manufacturer's instructions (DP430, Tiangen Biotech, China). The extracted RNA was reverse-transcribed into cDNA using the FastQuant RT Kit (with gDNase) (KR116, Tiangen Biotech, China). The transcriptional levels of plnABCDEF, pfs, and luxS were realized by CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) using SYBR Green PCR master mix (FP205, Tiangen Biotech, China). The reaction mixture consists of 10 μl 2×SuperReal PreMix Plus, 0.6 μl each of the forward and reverse primer, 1.0 μl cDNA (100 ng), and 7.8 μl nuclease-free H₂O. The amplification program was performed at 95°C for 15 min, followed by 39 cycles of 95°C for 10 s, 55°C for 20 s, and 72°C for 30 s. Primers were designed based on the genome sequence of L. paraplantarum RX-8 and synthesized by Beijing Genomics Institute (Table 1). 16S rRNA genes were used as the reference gene. The relative expression levels of target genes at each sampling point for L. paraplantarum RX-8 in mono-culture/co-culture were calculated by the ΔΔCt method.

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Publication 2023

Anabolism Bacteria Base Sequence Centrifugation Coculture Techniques DNA, Complementary Gene Expression Genes Genome L Cells Oligonucleotide Primers Ribosomal RNA Genes RNA, Ribosomal, 16S SYBR Green I Synthetic Genes Transcription, Genetic

Protein Extraction and Trypsin Digestion of L. paraplantarum RX-8

Cell samples of L. paraplantarum RX-8 in co-culture and mono-culture were collected at 24 h according to Section 2.5.1. The protein was extracted by using a lysis buffer (8 M urea, 50 mM Tris8.0, 1% NP40, 1% sodium deoxycholate, 5 mM dithiothreitol (DTT), 2 mM EDTA, 30 mM nicotinamide, and 3 μm trichostatin A), and, after sonication on ice, the total protein concentration of the supernatant, which was obtained by centrifugation (20,000 rpm, 10 min, 4°C), was determined by using a BCA Protein Assay kit. The protein sample was reduced by DTT (5 mM, 45 min, 30°C), later alkylated with 30 mM iodoacetamide (30 mM, 1 h, RT) in darkness, and then precipitated with ice-cold acetone. After being washed thrice with acetone, the precipitate was suspended in 0.1 M triethylammonium bicarbonate (TEAB) and digested with trypsin (1/25 protein mass, Promega) for 12 h at 37°C. Finally, the reaction was ended with 1% trifluoroacetic acid (TFA), and the resulting peptide was desalted with Strata X C18 SPE column (Phenomenex, Torrance, CA, USA) and vacuum-dried in Scanvac maxi-beta (Labogene, Alleroed, Denmark).

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Publication 2023

Acetone Biological Assay Buffers Centrifugation Coculture Techniques Cold Temperature Darkness Deoxycholic Acid, Monosodium Salt Dithiothreitol Edetic Acid Iodoacetamide L Cells Niacinamide Peptides Promega Proteins trichostatin A triethylammonium bicarbonate Trifluoroacetic Acid Trypsin Urea Vacuum

Top products related to «L Cells»

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific

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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

Lipofectamine 2000 by Thermo Fisher Scientific

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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.

Penicillin by Thermo Fisher Scientific

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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

Streptomycin by Thermo Fisher Scientific

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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

Penicillin streptomycin by Thermo Fisher Scientific

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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Rpmi 1640 by Thermo Fisher Scientific

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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.

L glutamine by Thermo Fisher Scientific

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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.

Rpmi 1640 medium by Thermo Fisher Scientific

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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.

Glutamax by Thermo Fisher Scientific

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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.

What are L Cells and how can they be used in research?

L Cells are a type of fibroblast-like cell line derived from mouse connective tissue. They are commonly used in cell biology research to study cellular processes, signaling pathways, and the effects of various treatments or stimuli. L Cells are versatile and can be used to investigate a wide range of topics related to cell function, differentiation, and interaction.

How can PubCompare.ai help optimize my L Cell research protocols?

PubComapre.ai allows you to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. The platform's AI-driven analysis can help researchers identify the most effective protocols related to L Cells for their specific research goals. By highlighting key differences in protocol effectiveness, PubCompare.ai enables you to choose the best option for reproducibility and accuracy, streamlining your L Cell research process and helping you get results faster.

What are some common challenges in working with L Cells, and how can PubCompare.ai address them?

Working with L Cells can present some challenges, such as ensuring consistent cell line characteristics, maintaining optimal culture conditions, and identifying the most effective protocols for your specific research needs. PubCompare.ai can assist by allowing you to compare a wide range of L Cell protocols from the literature, preprints, and patents, helping you identify the most reliable and efficient methods. This can save you time, reduce the risk of experimental variability, and ultimately improve the quality and reproducibility of your L Cell research.

Are there different types or variations of L Cells, and how can PubCompare.ai help me choose the right one?

Yes, there are several variations of L Cells, including L929 cells, L-M(TK-) cells, and others. These cell lines may have slightly different characteristics and be better suited for certain applications. PubCompare.ai's AI-driven insights can help you identify the most appropriate L Cell type for your research objectives, based on a comprehensive analysis of the literature and data. This can ensure you select the optimal L Cell line and protocols, leading to more reliable and meaningful results.

How can PubCompare.ai assist in specific applications of L Cells, such as in drug screening or tissue engineering?

L Cells have a variety of applications in research, including drug screening, tissue engineering, and the study of cellular processes. PubCompare.ai can help you navigate these different applications by providing a detailed comparison of the most effective protocols and products for your L Cell research. For example, in drug screening, the platform can identify the best L Cell-based assays and experimental designs to evaluate drug candidates. In tissue engineering, PubCompare.ai can assist in finding the optimal L Cell culture conditions and differentiation protocols to support tissue development and regeneration. By leveraging the power of AI, PubCompare.ai can streamline your L Cell research and help you achieve your specific goals more efficiently.

More about "L Cells"

L cells, also known as fibroblast-like cells, are a type of immortalized cell line derived from mouse connective tissue.
These cells are commonly used in cell biology research, particularly in the study of cell-cell interactions, signal transduction pathways, and cellular responses to various stimuli.
The culture and maintenance of L cells often involve the use of various cell culture media and supplements.
Fetal bovine serum (FBS) is a common additive that provides essential growth factors, while Dulbecco's Modified Eagle's Medium (DMEM) is a widely used basal medium that supports the growth and proliferation of L cells.
Lipofectamine 2000, a transfection reagent, can be used to introduce genetic material into L cells, enabling the study of gene expression and function.
Antibiotics, such as penicillin and streptomycin, are often included in L cell culture media to prevent bacterial contamination.
RPMI 1640 medium, another commonly used cell culture medium, can also be supplemented with L-glutamine to provide an essential amino acid for cell growth and metabolism.
Researchers working with L cells may leverage the power of AI-driven tools like PubCompare.ai to optimize their research protocols, locate the most effective protocols from the literature, preprints, and patents, and streamline their research processes.
By harnessing the insights provided by these AI-powered platforms, scientists can identify the most suitable protocols and products for their L cell research, ultimately leading to faster and more efficient results.