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L929 Cells

L929 cells are a commonly used mouse fibroblast cell line that has been extensively studied in biomedical research.
These cells are derivatives of normal subcutaneous areolar and adipose tissue, and are known for their versatility and robust growth characteristics.
Researchers can utilize PubCompare.ai's AI-driven platform to easily locate protocols from literature, preprints, and patents, while leverageing AI-driven comparisons to identify the best protocols and products for optimizing L929 cell research.
This innovative tool enhances reproducibility and research accuaracy, providing a valuable resource for scientists working with this important cell line.

Most cited protocols related to «L929 Cells»

1
Bone Marrow-Derived Macrophage Generation
Fresh or frozen bone marrow cells were used to generate BMDM as previously described [8] (link), using L929-cell conditioned medium (LCCM) as a source of granulocyte/macrophage colony stimulating factor [9] (link). The cells were resuspended in 10 ml bone marrow differentiation media (R20/30), which is RPMI1640 supplemented with 20% fetal bovine serum (Gibco, cat. 12657-029), 30% LCCM, 100 U/ml penicillin, 100 µg/ml streptomycin, and 2 mM L-glutamine. Cells were seeded in non-tissue culture treated Optilux Petri dishes (BD Biosciences) and incubated at 37°C in a 5% CO2 atmosphere. Four days after seeding the cells, an extra 10 ml of fresh R20/30 were added per plate and incubated for an additional 3 days. To obtain the BMDM, the supernatants were discarded and the attached cells were washed with 10 ml of sterile PBS. Ten ml of ice-cold PBS were added to each plate and incubated at 4°C for 10 minutes. The macrophages were detached by gently pipetting the PBS across the dish. The cells were centrifuged at 200× g for 5 minutes and resuspended in 10 ml of BMDM cultivation media (R10/5), which is composed of RPMI 1640, 10% fetal bovine serum, 5% LCCM and 2 mM L-glutamine. The cells were counted, seeded and cultivated in tissue culture plates 12 hours before any further experimental procedure.
Marim F.M., Silveira T.N., Lima DS J.r, & Zamboni D.S. (2010). A Method for Generation of Bone Marrow-Derived Macrophages from Cryopreserved Mouse Bone Marrow Cells. PLoS ONE, 5(12), e15263.
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Publication 2010
Atmosphere Bone Marrow Bone Marrow Cells Cells Common Cold Culture Media, Conditioned Fetal Bovine Serum Freezing Glutamine Granulocyte-Macrophage Colony-Stimulating Factor Hyperostosis, Diffuse Idiopathic Skeletal L929 Cells Macrophage Penicillins Sterility, Reproductive Streptomycin Tissues
2
Synchronization of Centrin-GFP Expressing Cells
L929, NIH 3T3, and HeLa-B cells were grown in DME medium (GIBCO) supplemented with 10% fetal calf serum.
Centrin cDNA was sub-cloned in the pEGFP-N1 vector (Clontech) and cells were transformed by electroporation. Stable clones expressing the centrin/GFP fusion protein were then isolated from each of the three parental cell lines, by using the limited dilution method in the presence of 500 μg/ml G418. Multiple independent clones, expressing ∼20 times the endogenous level of centrin, were kept for each of the three parental cell lines. The centrioles in all of the clones exhibited a similar behavior to that described here.
Synchronization in early G1 was accomplished by a single thymidine block of 20 h (5 mM thymidine for L929 and 2 mM for HeLa). Mitotic cells were then collected by shakeoff 8 or 20 h after releasing the block (for L929 and HeLa, respectively). Cells were then replated on coverslips and used 2 h later. Synchronization at the G1/S border was accomplished using the double-thymidine block technique. To determine the duration of S and G2 we incubated cells at various times after thymidine washout with 30 μM BrdU for 15 min and then analyzed them by FACS®. The maximum content of S cells (by BrdU incorporation) was reached 1–4 h after release. The maximum number of G2 cells (double DNA content and no BrdU incorporation) was observed ∼5 h after release in L929 cells and 9–10 h in HeLa cells.
Piel M., Meyer P., Khodjakov A., Rieder C.L, & Bornens M. (2000). The Respective Contributions of the Mother and Daughter Centrioles to Centrosome Activity and Behavior in Vertebrate Cells. The Journal of Cell Biology, 149(2), 317-330.
Publication 2000
antibiotic G 418 Bromodeoxyuridine Cell Lines Centrioles Clone Cells Cloning Vectors DNA, Complementary Electroporation Fetal Bovine Serum HeLa Cells L929 Cells NIH 3T3 Cells Parent Proteins Technique, Dilution Thymidine Trimethoprim-Sulfamethoxazole Combination
3
Propagation and Titration of West Nile Virus
BHK21 and L929 cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, 1 mM sodium pyruvate, antibiotic-antimycotic solution, and 1× nonessential amino acids (complete DMEM). WNV strain TX 2002-HC (WN-TX) was isolated by as previously described [11] (link). Working stocks of WN-TX were generated by a single round of amplification on Vero-E6 (ccl-81; ATCC) cells, and supernatants were collected, aliquoted, and stored at −80°C. Virus stocks were titered by a standard plaque assay on BHK21 cells as previously described [40] (link).
Suthar M.S., Ma D.Y., Thomas S., Lund J.M., Zhang N., Daffis S., Rudensky A.Y., Bevan M.J., Clark E.A., Kaja M.K., Diamond M.S, & Gale M J.r. (2010). IPS-1 Is Essential for the Control of West Nile Virus Infection and Immunity. PLoS Pathogens, 6(2), e1000757.
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Publication 2010
Amino Acids Antibiotics Biological Assay Cells Dental Plaque Eagle Fetal Bovine Serum Glutamine L929 Cells Pyruvate Sodium Strains Virus
4
Macrophage Isolation and Infection Protocols
wt macrophages were collected in four independent experiments with 8–10-wk-old C57BL/6 mice and two with C57BL/6 × 129Sv mice (six mice per experiment). Basal levels of gene expression in these two strains differed by ≥1.8-fold in only 24 of 13,029 genes 21. Macrophages were also collected in two independent experiments from iNOS-deficient mice 22, phox91-deficient mice 23, and mice deficient in both enzymes 19, all on the C57BL/6 background, and studied side-by-side with wt macrophages. Marrow cells flushed from femurs differentiated into macrophages over 6–8 d in DMEM containing 0.58 g/liter l-glutamine, 1 mM Na-pyruvate, 10% FBS, 10 mM Hepes buffer, 100 U/ml penicillin G, 100 μg/ml streptomycin, and 20% L929 cell-conditioned medium 24. 48 h before infection, the monolayers were washed with PBS and fresh medium (DMEM, 0.58 g/liter l-glutamine, 1 mM Na-pyruvate, 10% FBS, 10 mM Hepes buffer, and 10% L929 cell-conditioned medium) containing 0 or 100 U/ml recombinant mouse IFN-γ (Genentech). 2 × 105 macrophages per well in 24-well plates or 2 × 107 macrophages in 175 cm2 flasks were infected or not with 106 (plates) or 108 (flasks) CFUs of Mtb from early log phase cultures of a low-passage clinical isolate (strain 1254; American Type Culture Collection 51910).
Ehrt S., Schnappinger D., Bekiranov S., Drenkow J., Shi S., Gingeras T.R., Gaasterland T., Schoolnik G, & Nathan C. (2001). Reprogramming of the Macrophage Transcriptome in Response to Interferon-γ and Mycobacterium tuberculosis : Signaling Roles of Nitric Oxide Synthase-2 and Phagocyte Oxidase. The Journal of Experimental Medicine, 194(8), 1123-1140.
Publication 2001
Buffers Cells Culture Media, Conditioned Enzymes Femur Gene Expression Genes Glutamine HEPES IFNG protein, mouse Infection L929 Cells Macrophage Marrow Mice, Inbred C57BL Mus NOS2A protein, human Penicillin G Pyruvate Strains Streptomycin
5
Cytotoxicity Screening of Compounds 1-5
Compounds 1−5 were dissolved as described in the previous section. The cytotoxicity assay was initially performed against the cell lines L929 (mouse fibroblasts), as well as KB 3.1 (human papillomavirus-related endocervical adenocarcinoma), as described previously [16 (link)]. A detailed protocol, as well as sources of the cell lines, is given in the Supporting Information.
After incubating the cell lines with a serial dilution of the test compounds (final range: 37 to 0.6 × 10−3 µg/mL) for five days, the cells were dyed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), which is only converted to its purple formazan derivative by living cells. Then, the intensity of the purple derivative in relation to cells without additive (set to 100% viability) for each concentration of a test compound was quantified. For this, the absorption at 595 nm was measured using a microplate reader to calculate the percentage of cell viability. From this, the half-maximum inhibitory concentration (IC50, in µM) was calculated.
If an inhibition of cell viability with an IC50 < 50 µM was observed, further cell lines were subjected to the test compounds: PC-3 (human prostate adenocarcinoma), SK-OV-3 (human ovary adenocarcinoma), MCF-7 (human breast adenocarcinoma), A431 (human squamous carcinoma) and A549 (human lung carcinoma).
Becker K., Wessel A.C., Luangsa-ard J.J, & Stadler M. (2020). Viridistratins A−C, Antimicrobial and Cytotoxic Benzo[j]fluoranthenes from Stromata of Annulohypoxylon viridistratum (Hypoxylaceae, Ascomycota). Biomolecules, 10(5), 805.
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Publication 2020
Adenocarcinoma Biological Assay Breast Bromides Cell Lines Cells Cell Survival Cytotoxin diphenyl Endocervix Fibroblasts Formazans Homo sapiens Human Papillomavirus L929 Cells Lung Cancer Mus Ovary Prostate Psychological Inhibition Squamous Cell Carcinoma Technique, Dilution Tetrazolium Salts

Most recents protocols related to «L929 Cells»

1
Infecting Mouse Macrophages with Mycobacterium tuberculosis
Mouse hematopoietic stem cells were isolated as described in Banaiee et al., 2006 (link). Hematopoietic cells were differentiated by culturing for 7 days in Dulbecco’s Modified Eagle Medium (DMEM) with 10% FBS, 2 mM L-glutamine, and 1 mM pyruvate (DMEM complete). DMEM complete media was supplemented with 20% L929 cell supernatant (as a source of macrophage colony stimulating factor (M-CSF), 100 U/mL final concentration), 10 units/ml penicillin, and 10 units/ml streptomycin. Following differentiation, BMDMs were washed with PBS, resuspended in DMEM complete with 10% L929 cell supernatant, and plated for infection the following day. Single cell suspensions of Mtb in DMEM complete with 10% L929 added to macrophages at a MOI of 5, 10, 20, or 40, and plates were spun for 5 min at 51 x g. The MOI was verified by plating the inoculum. At 4 hpi, macrophages were washed 3 times with DMEM to remove extracellular bacteria. To enumerate CFU, at specified time points macrophages were lysed with 0.06% sodium dodecyl sulfate (SDS) in water and serially diluted in PBS. The cell lysates were plated on 7H11 agar plates supplemented with OADC and glycerol, and CFU were counted after 14–21 days. For qPCR, macrophages were lysed in TRI Reagent (Zymo Research, R2050-1-50), and total RNA was extracted.
Mittal E., Roth A.T., Seth A., Singamaneni S., Beatty W, & Philips J.A. (2023). Single cell preparations of Mycobacterium tuberculosis damage the mycobacterial envelope and disrupt macrophage interactions. eLife, 12, e85416.
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Publication 2023
Agar Bacteria Cells Eagle Glutamine Glycerin Hematopoietic System Infection L929 Cells Macrophage Macrophage Colony-Stimulating Factor Mus Penicillins Pyruvate Stem Cells, Hematopoietic Streptomycin Sulfate, Sodium Dodecyl
2
Cytotoxicity Evaluation of Titanium Dioxide Membranes
The L929 mouse fibroblast cell
line was used for the cytotoxicity assay. Cells were thawed from the
stock and cultured in the medium consisting of 90% DMEM-F12 and 10%
FBS with a supplement of 2 mM l-glutamine in a humidified
5% CO2/95% air environment at 37 °C. Meanwhile, a
representative titanium dioxide membrane with 2 cm2 surface
area was immersed in the cell culture medium and incubated for 72
h at 37 °C. L929 cells were harvested at 80% confluency and seeded
in 96-well plates at 5000 cells/well and then incubated overnight.
The next day, the medium was removed and replaced with the nanomembrane
extract and then allowed for another overnight incubation. 10% DMSO
in cell culture medium was used as the positive control. The next
day, the medium was replaced with 100 μL of fresh medium and
13 μL of MTT solution (1 mg/mL). After 4 h of incubation in
the dark at 37 °C, the medium was removed again, and 200 μL
of isopropanol-HCl was added to each well to dissolve the blue formazan
crystals. After 10 min, the wells were read at 570 nm wavelength,
and the percentage of cell viability was calculated. Cell viability
was defined as 100% for the MTT assay control, and cell growth was
directly proportional to absorbance at a wavelength of 570 nm.
Kilicarslan B., Sardan Ekiz M, & Bayram C. (2023). Electrostatic Repulsive Features of Free-Standing Titanium Dioxide Nanotube-Based Membranes in Biofiltration Applications. Langmuir, 39(9), 3400-3410.
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Publication 2023
Biological Assay Cell Culture Techniques Cells Cell Survival Cytotoxin Dietary Supplements Fibroblasts Glutamine Isopropyl Alcohol L929 Cells Mus Tissue, Membrane titanium dioxide
3
Cytotoxicity Evaluation of Materials
Cytotoxicity was
evaluated according
to the ISO10993-5-2009 regulation, where the L929 cell line was chosen,
and an MTT cell viability assay was performed. To obtain extraction
solutions, samples were cut into square pieces (2 × 2 cm2) and sterilized by UV irradiation for 40 min. Then, samples
were placed in six-well tissue culture plates and filled with a culture
medium (DMEM) with an extraction ratio of 4 cm2/0.666 mL
at 37 °C in a 5% CO2 incubator for 24 h. L929 cells
were seeded into 96-well culture plates at a density of 6 × 103 cells/well in 100 μL of a culture medium and incubated
for 24 h at 37 °C in a humidified atmosphere containing 5% CO2 in air. After this time, culture media were replaced with
100 μL of the sample and control group extracts (the extraction
medium without the sample and only a glass slide were used as control
groups). Cells were examined microscopically after 24 h of incubation
to assess the general morphology of cells. Then, the cells were incubated
with 10 μL of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide solution (MTT, 5 mg/mL, Sigma-Aldrich, Germany) for 3 h. Formazan
crystals were dissolved in 100 μL of dimethyl sulfoxide, and
the absorbance (OD) was measured with a microplate reader (Promega
Multireader Glomax) at 560 nm. Cell viability was calculated by the
following equation
Experiments
were performed in triplicate,
and mean OD values were normalized to the control group and represented
as cell viability (%).
Celik N., Sahin F., Ozel S.S., Sezer G., Gunaltay N., Ruzi M, & Onses M.S. (2023). Self-Healing of Biocompatible Superhydrophobic Coatings: The Interplay of the Size and Loading of Particles. Langmuir, 39(9), 3194-3203.
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Publication 2023
Atmosphere Biological Assay Cells Cell Survival Culture Media Cytotoxin L929 Cells Sulfoxide, Dimethyl Tissues Ultraviolet Rays
4
Cytotoxicity of Naproxen-based Ionic Liquids
The cytotoxicity of the naproxen-based
ILs of L929 cells in vitro was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide (MTT) assay. Cells were seeded at a density of 1 × 104 cells·mL–1 cells/well (200 μL)
in DMEM containing 10% fetal bovine serum and 1% antibiotic solution
in 96-well plates for 24–48 h at 37 °C. In serum-free
DMEM, wells were rinsed with sterile PBS and treated with various
concentrations of the API-IL samples. Each sample was reproduced three
times, and the cells were cultured for 24 h at 37 °C. Twenty
microliters of MTT (5 mg·mL–1) was added to
each well, and the cells were incubated for 4 h until purple precipitates
were visible under a microscope. Finally, the medium was gently aspirated
from the wells and rinsed with 1× PBS (200 μL). Then we
added dimethylsulfoxide (DMSO) (100 μL) to dissolve the formazan
crystals and shook the plate for 5 min. Using a microplate reader
(Thermo Fisher Scientific, USA), the absorbance value of each well
was measured at 490 nm, and the cell viability and IC50 value were calculated using GraphPad Prism 5.0 (USA).
Tang Y., Yang K., Zhao S., Chen Q., Qin L, & Qin B. (2023). Evaluation of Solubility, Physicochemical Properties, and Cytotoxicity of Naproxen-Based Ionic Liquids. ACS Omega, 8(9), 8332-8340.
Publication 2023
Antibiotics Biological Assay Cells Cell Survival Cytotoxin Fetal Bovine Serum L929 Cells Microscopy Naproxen prisma Serum Sterility, Reproductive Sulfoxide, Dimethyl Tremor
5
Thermally Responsive Gauze Dressing

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Publication 2023
Acetone Animals Biological Assay Biopharmaceuticals Cell Survival Cytotoxin Enzyme-Linked Immunosorbent Assay Factor XII Fingers fluorexon Gossypium hexamethyldisiloxane L929 Cells Lactate Dehydrogenase Males Microcapsules Obstetric Delivery prothrombin fragment 1.2 Rats, Sprague-Dawley sodium silicate Sulfuric Acids Temperature Regulations, Body

Top products related to «L929 Cells»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
5
Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
6
L929 cell by American Type Culture Collection
Sourced in United States, China
L929 cells are a well-established and commonly used mouse fibroblast cell line. They are derived from the subcutaneous areolar and adipose tissue of a 100-day-old male C3H/An mouse. L929 cells are a widely utilized tool in various research applications.
7
L glutamine by Thermo Fisher Scientific
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L-glutamine is an amino acid that is commonly used as a dietary supplement and in cell culture media. It serves as a source of nitrogen and supports cellular growth and metabolism.
8
Rpmi 1640 by Thermo Fisher Scientific
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
9
Rpmi 1640 medium by Thermo Fisher Scientific
Sourced in United States, China, Germany, United Kingdom, Japan, France, Canada, Australia, Italy, Switzerland, Belgium, New Zealand, Spain, Israel, Sweden, Denmark, Macao, Brazil, Ireland, India, Austria, Netherlands, Holy See (Vatican City State), Poland, Norway, Cameroon, Hong Kong, Morocco, Singapore, Thailand, Argentina, Taiwan, Province of China, Palestine, State of, Finland, Colombia, United Arab Emirates
RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
10
Dulbecco modified eagle medium (dmem) by Merck Group
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DMEM (Dulbecco's Modified Eagle's Medium) is a commonly used cell culture medium formulated to support the growth and maintenance of various cell lines. It provides a balanced salt solution and essential nutrients required for cell proliferation. DMEM is suitable for use in a wide range of cell culture applications.

More about "L929 Cells"

L929 cells, a widely used mouse fibroblast cell line, have been extensively studied in biomedical research due to their versatility and robust growth characteristics.
These cells are derivatives of normal subcutaneous areolar and adipose tissue, making them a valuable model for various research applications.
To enhance the reproducibility and accuracy of L929 cell research, scientists can utilize PubCompare.ai's innovative AI-driven platform.
This tool allows researchers to easily locate protocols from literature, preprints, and patents, while leveraging AI-driven comparisons to identify the best protocols and products for optimizing their L929 cell studies.
When working with L929 cells, it is important to consider the culture conditions, including the use of Fetal Bovine Serum (FBS), Dulbecco's Modified Eagle Medium (DMEM), and supplementation with L-glutamine, penicillin, and streptomycin.
These components help support the growth and maintenance of L929 cells in vitro.
Additionally, researchers may also utilize RPMI 1640 medium, another commonly used cell culture medium, for culturing L929 cells.
The combination of these media and supplements ensures a suitable environment for the cells to thrive and enables reliable and reproducible experiments.
By leveraging PubCompare.ai's AI-driven platform, scientists can streamline their L929 cell research, enhance reproducibility, and improve the accuracy of their findings, ultimately advancing the understanding and applications of this important cell line.