> Anatomy > Cell > Myocytes, Cardiac

Myocytes, Cardiac

Q: What are the key functions of cardiac myocytes?

Cardiac myocytes, also known as cardiomyocytes, are the highly specialized muscle cells that make up the heart. They are responsible for the pumping action of the heart, allowing it to contract and relax in a coordinated manner to circulate blood throughout the body. Cardiac myocytes are striated, meaning they have a banded appearance under the microscope, and they contain a single, centrally located nucleus. They are also electrically excitable, responding to electrical signals that trigger the contraction of the heart muscle. Understanding the biology and function of cardiac myocytes is essential for the study of cardiac physiology, development, and disease, as well as the development of therapies for cardiovascular conditions.

Q: How can PubCompare.ai assist with research on cardiac myocytes?

PubCompare.ai can help optimize your research on cardiac myocytes in several ways. First, the platform allows you to screen protocol literature more efficiently, leveraging AI to pinpoint critical insights. This can help researchers identify the most effective protocols related to myocytes, cardiac, for their specific research goals. The platform's AI-driven analysis can also highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your cardiac studies. By using PubCompare.ai, you can enhance the quality and impact of your research on cardiac myocytes.

Q: What are some common applications of cardiac myocyte research?

Cardiac myocyte research has a wide range of applications in the field of cardiovascular science and medicine. Researchers often utilize advanced techniques, such as cell culture, electrophysiology, and genetic manipulation, to gain insights into the structure, metabolism, and role of cardiac myocytes in heart function and disease. This knowledge is essential for understanding cardiac physiology, development, and the pathogenesis of cardiovascular conditions. Cardiac myocyte research also plays a crucial role in the development of new therapies for heart-related diseases, such as myocardial infarction, heart failure, and arrhythmias.

Q: Are there different types or variations of cardiac myocytes?

Yes, there are several different types and variations of cardiac myocytes. While the majority of cardiac muscle cells are working myocytes responsible for the pumping action of the heart, there are also specialized cardiac myocytes that play important roles in cardiac conduction and regulation. These include pacemaker cells, which generate the electrical impulses that trigger heart contractions, and Purkinje fibers, which rapidly conduct these impulses throughout the ventricular myocardium. Additionally, there are differences in the characteristics and properties of cardiac myocytes based on their location within the heart (e.g., atrial vs. ventricular myocytes) and their stage of development (e.g., fetal vs. adult myocytes). Understanding these variations is crucial for studying cardiac physiology and pathology in depth.

Cardiac myocytes, also known as cardiomyocytes, are the muscle cells that make up the heart.
These highly specialized cells are responsible for the pumping action of the heart, allowing it to contract and relax in a coordinated manner to circulate blood throughout the body.
Cardiac myocytes are striated, meaning they have a banded appearance under the microscope, and they contain a single, centrally located nucleus.
They are electrically excitable, responding to electrical signals that trigger the contraction of the heart muscle.
Understanding the biology and function of cardiac myocytes is essential for the study of cardiac physiology, development, and disease, as well as the development of therapies for cardiovascular conditions.
Researches on cardiac myocytes often utilize advanced techniques, such as cell culture, electrophysiology, and genetic manipulation, to gain insights into their structure, metabolism, and role in heart function and disease.

Most cited protocols related to «Myocytes, Cardiac»

Culturing Adult Cardiomyocytes for Prolonged Experiments

Protocol full text hidden due to copyright restrictions

Open the protocol to access the free full text link

Publication 2011

Adult Contracture Digestion Enzymes isolation Muscle Cells Muscle Contraction Myocytes, Cardiac Physical Examination Primary Cell Culture Sarcoplasmic Reticulum Stimulations, Electric Therapies, Investigational Trypan Blue

Cardiomyocyte Ablation and Lineage Tracing in Zebrafish

Chamber-specific ablation and reporter lines were generated using the standard I-SceI meganuclease transgenesis technique (details in Methods). To perform ventricular cardiomyocyte ablation, Tg(vmhc:mCherry-NTR) zebrafish were treated with 5 mM MTZ as previously described^{9 (link)}. For lineage tracing experiments, Tg(vmhc:mCherry-NTR;amhc:CreERT2;β-act2:RSG) zebrafish were treated with 10 µM 4-hydroxytamoxifen as previously described^{5 (link)}. For Notch inhibition studies, zebrafish were treated with 100 µM DAPT. Live imaging, heart contraction, immunofluorescence, and whole mount in situ hybridization were performed as described in Methods.

Zhang R., Han P., Yang H., Ouyang K., Lee D., Lin Y.F., Ocorr K., Kang G., Chen J., Stainier D.Y., Yelon D, & Chi N.C. (2013). In Vivo Cardiac Reprogramming Contributes to Zebrafish Heart Regeneration. Nature, 498(7455), 497-501.

Publication 2013

1,2-dilinolenoyl-3-(4-aminobutyryl)propane-1,2,3-triol CCL4 protein, human Fluorescent Antibody Technique Heart Ventricle hydroxytamoxifen In Situ Hybridization Myocardial Contraction Myocytes, Cardiac Psychological Inhibition Zebrafish

Cardiac Fibroblast Reprogramming and In Vivo Validation

All experimental procedures with animals were approved by the Institutional Animal Care and Use Committee at UT Southwestern Medical Center.
Adult TTFs and CFs were isolated by an explanting method in which fibroblasts migrate from minced tissue and grow in fibroblast growth medium. Fibroblasts were transduced with a mixture of polybrene (Sigma; 6μg/ml) and freshly made retroviruses expressing transcription factors generated in Platinum E cells (Cell Biolabs). Twenty-four hours after viral transduction, the viral medium was changed to a cardiac induction medium. Medium was changed every two days. Expression of cardiac genes was analyzed by flow cytometry, microarray, and immunocytochemistry.
Adult mice, 8–10 weeks old, underwent either a sham operation or ligation of the LAD. Concentrated retroviruses (~10^8~9 pfu viruses) were injected into the border zone using a gastight 1710 syringe (Hamilton). Cardiac function was assessed using echocardiography and MRI. Hearts were harvested from euthanized animals for histological studies.
Isolation of cardiomyocytes, electrophysiological measurements, histology and immunohistochemistry were performed as described previously^{2 (link), 33 (link)–37 (link)}.

Song K., Nam Y.J., Luo X., Qi X., Tan W., Huang G.N., Acharya A., Smith C.L., Tallquist M.D., Neilson E.G., Hill J.A., Bassel-Duby R, & Olson E.N. (2012). Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature, 485(7400), 599-604.

Publication 2012

Adult Animals Animals, Laboratory Cells Echocardiography Fibroblasts Flow Cytometry Gene Expression Heart Immunocytochemistry Immunohistochemistry Institutional Animal Care and Use Committees isolation Ligation Microarray Analysis Mus Myocytes, Cardiac Platinum Polybrene Retroviridae Syringes Tissues Transcription Factor Virus

Subpopulation Analysis of Cardiac and Neural Cells

All barcodes labelled in the global object as cardiomyocytes, fibroblasts and neural cells were selected for further subpopulation analyses. Additional cell population-specific filtering criteria were applied to nuclei as follows: cardiomyocyte counts (n_counts <12,500), genes (n_genes <4,000), mitochondrial genes (percent_mito <1%), ribosomal genes (percent_ribo <1%) and scrublet score (scrublet_score <0.25); FB mitochondrial genes (percent_mito <1%), ribosomal genes (percent_ribo <1%); neuronal cell genes (n_genes <4000), mitochondrial genes (percent_mito <1%), ribosomal genes (percent_ribo <1%). Total and CD45⁺ cells were excluded in the atrial and ventricular cardiomyocytes datasets and did not contribute to subpopulation analysis. No further filtering of FBs or neuronal cell total and CD45⁺ cells was applied. Cardiomyocytes and FBs were then further split into two groupings based on the region of origin: (1) left and right atrium, and (2) left and right ventricles, apex and interventricular septum.
Donor effects were aligned as described in step (1) above. For FB and neuronal cells, sources were aligned as described in step (3) above. Leiden clustering and UMAP visualization were performed for identifying subpopulations and visualization^{86 (link)}. Differentially expressed genes were calculated using the Wilcoxon rank sum test. Genes were ranked by score.

Litviňuková M., Talavera-López C., Maatz H., Reichart D., Worth C.L., Lindberg E.L., Kanda M., Polanski K., Heinig M., Lee M., Nadelmann E.R., Roberts K., Tuck L., Fasouli E.S., DeLaughter D.M., McDonough B., Wakimoto H., Gorham J.M., Samari S., Mahbubani K.T., Saeb-Parsy K., Patone G., Boyle J.J., Zhang H., Zhang H., Viveiros A., Oudit G.Y., Bayraktar O.A., Seidman J.G., Seidman C.E., Noseda M., Hubner N, & Teichmann S.A. (2020). Cells of the adult human heart. Nature, 588(7838), 466-472.

Publication 2020

Atrium, Right Cell Nucleus Cells Fibroblasts Genes Genes, Mitochondrial Heart Atrium Heart Ventricle Mitomycin Myocytes, Cardiac Neurons Population Group Reproduction Ribosomes Strains Tissue Donors Ventricles, Right Ventricular Septum

Modeling Myocardial Fiber Orientation

To assign fiber orientation throughout the myocardium according to the rules R1–R6 above, the LDRB algorithm takes four functions as inputs, representing the desired α and β angles within the septum (s) and the ventricular walls (w). The angles α and β are in degrees and d is the transmural depth normalized from 0 to 1.

α_{s} (d) = α_{endo} (1 - d) - α_{endo} * d

α_{w} (d) = α_{endo} (1 - d) + α_{epi} * d

β_{s} (d) = β_{endo} (1 - d) + β_{endo} * d

β_{w} (d) = β_{endo} (1 - d) + β_{epi} * d

If non-linear interpolation functions are desired, these functions need to satisfy α_w(0) = α_s(0) = −α_s(1) modulo 180° and β_w(0) = β_s(0) = −β_s(1) modulo 180°. These conditions ensure that the coordinate systems used for assigning fiber orientation on the endocardium of Ω will be appropriately oriented, regardless of whether orientations are parametrized in the LV or RV wall.
The LDRB algorithm also requires the definition of the following surfaces in order to assign Dirichlet boundary conditions:

∂Ω_apex: the apex of the ventricles

∂Ω_base: the base of the ventricles

∂Ω_epi: the epicardial surface of the ventricles

∂Ω_lv: the endocardial surface of the LV

∂Ω_rv: the endocardial surface of the RV

For most studies, the surface ∂Ω_base can be extracted by taking a cutting plane at the apicobasal junction, and the surface ∂Ω_apex can be extracted by finding the point lying closest to the ventricular apex of Ω. The surfaces ∂Ω_epi, ∂Ω_lv, and ∂Ω_rv can then be extracted by choosing an arbitrary point on the epicardium, LV endocardium, and RV endocardium, then iteratively expanding from these three points along the surface of Ω until intersection with ∂Ω_base.

Bayer J.D., Blake R.C., Plank G, & Trayanova N.A. (2012). A Novel Rule-Based Algorithm for Assigning Myocardial Fiber Orientation to Computational Heart Models. Annals of biomedical engineering, 40(10), 2243-2254.

Publication 2012

Endocardium Epicardium Fibrosis Heart Ventricle Mental Orientation Myocytes, Cardiac

Most recents protocols related to «Myocytes, Cardiac»

Cellular Uptake and Cytotoxicity of Cy5.5-Labeled Nanoparticles

The cellular uptake of Cy5.5-CNPs was investigated in three types of normal cells, such as breast cancer cells (4T1), rat cardiomyocytes (H9C2), mouse fibroblasts (L929) and macrophages (Raw264.7). Each cell was incubated with Cy5.5-CNPs (100, 225 or 900 μg/ml) for 4 or 24 h. After treatment, cells were fixed with 4% paraformaldehyde for 15 min, and stained with 4′,6-diamidino-2-phenylindole (DAPI) for 10 min. The cellular uptake was observed using a Leica TCS SP8 confocal laser-scanning microscope (CLSM; Leica Microsystems GmbH; Wetzlar, Germany). Quantitative analyses of the fluorescence images were performed using ImageJ software (NIH, Bethesda, MD, USA). The cytotoxicity was assessed by the Cell Counting Kit-8 (CCK-8) assay. Briefly, 5 × 10⁴ 4T1, H9C2, L929 and Raw264.7 cells were seeded into 96-well cell culture plates. Then, each cell was treated with different concentration of Cy5.5-CNPs ranging from 0 to 900 μg/ml. After 24 h of incubation, the cells were further incubated with cell culture medium containing 10% of CCK-8 solution for 20 min. Finally, the cell viability was analyzed using a microplate reader (VERSAmaxTM; Molecular Devices Corp., USA) with a wavelength of 450 nm.

Chang H., Yhee J.Y., Jeon S., Shim M.K., Yoon H.Y., Lee S, & Kim K. (2023). In vivo toxicity evaluation of tumor targeted glycol chitosan nanoparticles in healthy mice: repeated high-dose of glycol chitosan nanoparticles potentially induce cardiotoxicity. Journal of Nanobiotechnology, 21, 82.

Publication 2023

Biological Assay Breast Carcinoma Cell Culture Techniques Cells Cell Survival Culture Media CY5.5 cyanine dye Cytotoxin Fibroblasts Fluorescence Macrophage Medical Devices Microscopy, Confocal Mus Myocytes, Cardiac paraform RAW 264.7 Cells

Glycol chitosan-based immunomodulatory nanocarrier

Glycol chitosan (Mw = 250 kDa, 5β-cholanic acid, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma Aldrich (St. Louis, MO, USA). Flamma 648 NHS ester was purchased from BioActs (Incheon, Republic of Korea). Cell counting kit-8 (CCK-8) was purchased from Vitascientific (Beltsville, MD, USA). Tem grid (Carbon Film 200 Mesh copper) was purchased from Electron Microscopy Sciences (Hatfield, PA, USA). H9C2 (Rat cardiomyocyte), L929 (mouse fibroblast) and Raw264.7 (macrophage) cell lines were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA). Fetal bovine serum (FBS), streptomycin, penicillin and RPMI 1640 medium were purchased from WELGENE Inc. (Daegu, Republic of Korea). Antibodies against mouse TNF-α (cat# 109,828), mouse IL1b, mouse IL6 and mouse β-actin were purchased from BioLegend (San Diego, CA, USA).

Publication 2023

Actins Antibodies Carbodiimides Carbon Cell Lines cholanic acid Copper Electron Microscopy Esters Fetal Bovine Serum Fibroblasts glycol-chitosan Interleukin-1 Macrophage Mus Myocytes, Cardiac N-hydroxysuccinimide Penicillins Streptomycin Tumor Necrosis Factor-alpha

Immunofluorescent Characterization of iPSC-Derived Cardiomyocytes

IPSc-derived cardiomyocytes were cultured on glass slides coated with Synthemax II-SC (Corning) in RPMI-1640/B-27 with Y27632 (5 μM) media for 2 days. Cells were fixed with 4% formaldehyde for 15 min at room temperature. Fixed cells were blocked in antibody buffer (5% BSA, 0.1% Tween-20 in PBS) for 1 h at room temperature. Following blocking, cells were incubated overnight at 4 °C with cardiac troponin-T antibody (abcam, ab45932, 1:200), α-Actinin antibody (Sigma, A7811, 1:800), and/or Connexin 43 (Cell Signaling Technology, 1:100) in antibody buffer. After the overnight incubation, cells were washed three times in antibody buffer. Following washing, cells were incubated with Alexa-Fluor conjugated secondary antibodies (Thermo Fisher Scientific, Alexa 488 Donkey anti-Rabbit IgG and Alexa 594 Goat anti-Ms IgG1) at a 1:1,000 dilution in antibody buffer for 1 h at room temperature. Nuclei were stained by DAPI at 1 μg/ml and Wheat Germ Agglutinin (Thermo Fisher Scientific, W21404) was used to stain cell membrane for 10 min at room temperature in antibody buffer. Following washing in PBS to remove unbound complexes, sarcomeres were analyzed using a Zeiss LSM510 confocal microscope. Images were processed with Zeiss software (Axiovision Rel4.8 and Zen Blue). Circularity measurements were made by comparing cardiomyocyte length to width and were expressed as a circularity index whereby circularity index = width/length^{15 (link)}. Sarcomere distance measurements were made with ImageJ^{15 (link)}. All measurements were made with a double-blinding method.

Hsueh Y.C., Pratt R.E., Dzau V.J, & Hodgkinson C.P. (2023). Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Scientific Reports, 13, 3920.

Publication 2023

Actinin Alexa594 anti-IgG Antibodies Buffers Cardiac Arrest Cell Nucleus DAPI Equus asinus Formaldehyde GJA1 protein, human Goat Heart IgG1 Immunoglobulins Induced Pluripotent Stem Cells Microscopy, Confocal Myocytes, Cardiac Plasma Membrane Rabbits Sarcomeres Stains Technique, Dilution Troponin T Tween 20 Wheat Germ Agglutinins Y 27632

Electrophysiological Recordings of iPSC-Derived Cardiomyocytes

IPSc-derived cardiomyocytes were cultured on glass slides coated with BG iMatrix-511 (Biogems, 0.5 μg/cm²) in RPMI-1640/B-27 with Y27632 (5 μM) media for 2 days. The microelectrodes (Sutter Instruments, BF150-110-10) were made via a micropipette puller (Sutter Instrument, P-87) to create electrodes at ~ 3 MΩ in Tyrode's solution (140 mM NaC1, 5.4 mM KCl, 1.8 mM CaCl₂, 1.05 mM MgCl₂, 0.33 mM NaH₂PO₄, 5 mM HEPES and 10 mM glucose; pH was adjusted to 7.4 with NaOH). Current-clamp mode was used to record action potential via an Axopatch 200B amplifier running software Calmpex 8.2.

Hsueh Y.C., Pratt R.E., Dzau V.J, & Hodgkinson C.P. (2023). Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Scientific Reports, 13, 3920.

Publication 2023

Action Potentials Glucose HEPES Induced Pluripotent Stem Cells Magnesium Chloride Microelectrodes Myocytes, Cardiac Tyrode's solution Y 27632

Cardiomyocyte Characterization by Flow Cytometry

iPSc-derived cardiomyocytes were removed from the tissue culture plate with Trypsin–EDTA (Thermo Fisher Scientific, 0.25%) and fixed in a solution containing 1% formaldehyde (Thermo Fisher Scientific) and 90% methanol (Sigma). Fixed cells were incubated with antibodies for cardiac troponin-T (Thermo Fisher Scientific, MA5-12,960, 1:200) or MLC2v-PE (Mitenyi Biotec, 130–119-581, 1:50) in FACS buffer (PBS with 0.5% BSA) for 45 min at room temperature. Where necessary, primary antibodies were removed and the cells incubated with an Alexa 488 Goat anti-Ms IgG1 (Thermo Fisher Scientific, A-21121, 1:1,000) in FACS buffer for an additional 30 min at room temperature. Cells were washed three times in FACS buffer and analyzed on a BD Sciences FACSCantoII. Compensation was performed by FlowJo. Singlets were analyzed via gating on the FSC-H and FSC-L channels.

Hsueh Y.C., Pratt R.E., Dzau V.J, & Hodgkinson C.P. (2023). Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Scientific Reports, 13, 3920.

Publication 2023

Antibodies Buffers Cells Edetic Acid Formaldehyde Goat Heart IgG1 Induced Pluripotent Stem Cells Methanol Myocytes, Cardiac Tissues Troponin T Trypsin

Top products related to «Myocytes, Cardiac»

Fetal bovine serum (fbs) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal

Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific

Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica

DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.

Lipofectamine 2000 by Thermo Fisher Scientific

Sourced in United States, China, Germany, United Kingdom, Canada, Japan, France, Italy, Switzerland, Australia, Spain, Belgium, Denmark, Singapore, India, Netherlands, Sweden, New Zealand, Portugal, Poland, Israel, Lithuania, Hong Kong, Argentina, Ireland, Austria, Czechia, Cameroon, Taiwan, Province of China, Morocco

Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.

Penicillin streptomycin by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom, China, Canada, France, Japan, Australia, Switzerland, Israel, Italy, Belgium, Austria, Spain, Gabon, Ireland, New Zealand, Sweden, Netherlands, Denmark, Brazil, Macao, India, Singapore, Poland, Argentina, Cameroon, Uruguay, Morocco, Panama, Colombia, Holy See (Vatican City State), Hungary, Norway, Portugal, Mexico, Thailand, Palestine, State of, Finland, Moldova, Republic of, Jamaica, Czechia

Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.

Trizol reagent by Thermo Fisher Scientific

Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand

TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.

Collagenase type 2 by Worthington

Sourced in United States, United Kingdom, Jersey, Germany, Japan, Switzerland, Canada, Australia, France

Collagenase type II is an enzyme used in cell and tissue culture applications. It is responsible for the breakdown of collagen, a structural protein found in the extracellular matrix. This enzyme is commonly used to facilitate the dissociation of cells from tissues during cell isolation and harvesting procedures.

In situ cell death detection kit by Roche

Sourced in Germany, United States, Switzerland, China, United Kingdom, France, Canada, Belgium, Japan, Italy, Spain, Hungary, Australia

The In Situ Cell Death Detection Kit is a laboratory product designed for the detection of programmed cell death, or apoptosis, in cell samples. The kit utilizes a terminal deoxynucleotidyl transferase (TdT) to label DNA strand breaks, allowing for the visualization and quantification of cell death. The core function of this product is to provide researchers with a tool to study and analyze cell death processes.

Penicillin by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, China, France, Canada, Japan, Australia, Switzerland, Italy, Israel, Belgium, Austria, Spain, Brazil, Netherlands, Gabon, Denmark, Poland, Ireland, New Zealand, Sweden, Argentina, India, Macao, Uruguay, Portugal, Holy See (Vatican City State), Czechia, Singapore, Panama, Thailand, Moldova, Republic of, Finland, Morocco

Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.

Streptomycin by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama

Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.

Trypsin by Thermo Fisher Scientific

Sourced in United States, United Kingdom, China, Germany, Canada, Japan, France, Australia, Italy, Switzerland, Spain, Brazil, Belgium, Netherlands, Ireland, Singapore, Poland, Macao, India, Sweden, Denmark, Austria, Argentina, Israel, Lithuania

Trypsin is a proteolytic enzyme that hydrolyzes peptide bonds in proteins. It is commonly used in cell biology and molecular biology applications to facilitate cell detachment and dissociation.

What are the key functions of cardiac myocytes?

Cardiac myocytes, also known as cardiomyocytes, are the highly specialized muscle cells that make up the heart. They are responsible for the pumping action of the heart, allowing it to contract and relax in a coordinated manner to circulate blood throughout the body. Cardiac myocytes are striated, meaning they have a banded appearance under the microscope, and they contain a single, centrally located nucleus. They are also electrically excitable, responding to electrical signals that trigger the contraction of the heart muscle. Understanding the biology and function of cardiac myocytes is essential for the study of cardiac physiology, development, and disease, as well as the development of therapies for cardiovascular conditions.

How can PubCompare.ai assist with research on cardiac myocytes?

PubCompare.ai can help optimize your research on cardiac myocytes in several ways. First, the platform allows you to screen protocol literature more efficiently, leveraging AI to pinpoint critical insights. This can help researchers identify the most effective protocols related to myocytes, cardiac, for their specific research goals. The platform's AI-driven analysis can also highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy in your cardiac studies. By using PubCompare.ai, you can enhance the quality and impact of your research on cardiac myocytes.

What are some common applications of cardiac myocyte research?

Cardiac myocyte research has a wide range of applications in the field of cardiovascular science and medicine. Researchers often utilize advanced techniques, such as cell culture, electrophysiology, and genetic manipulation, to gain insights into the structure, metabolism, and role of cardiac myocytes in heart function and disease. This knowledge is essential for understanding cardiac physiology, development, and the pathogenesis of cardiovascular conditions. Cardiac myocyte research also plays a crucial role in the development of new therapies for heart-related diseases, such as myocardial infarction, heart failure, and arrhythmias.

Are there different types or variations of cardiac myocytes?

Yes, there are several different types and variations of cardiac myocytes. While the majority of cardiac muscle cells are working myocytes responsible for the pumping action of the heart, there are also specialized cardiac myocytes that play important roles in cardiac conduction and regulation. These include pacemaker cells, which generate the electrical impulses that trigger heart contractions, and Purkinje fibers, which rapidly conduct these impulses throughout the ventricular myocardium. Additionally, there are differences in the characteristics and properties of cardiac myocytes based on their location within the heart (e.g., atrial vs. ventricular myocytes) and their stage of development (e.g., fetal vs. adult myocytes). Understanding these variations is crucial for studying cardiac physiology and pathology in depth.

More about "Myocytes, Cardiac"

Cardiomyocytes, also known as cardiac muscle cells or myocytes, are the specialized cells that make up the heart's muscular tissue.
These highly intricate cells are responsible for the heart's pumping action, allowing it to contract and relax in a coordinated manner to circulate blood throughout the body.
Cardiomyocytes have a striated, banded appearance under the microscope and contain a single, centrally located nucleus.
They are electrically excitable, responding to electrical signals that trigger the contraction of the heart muscle.
Understanding the biology and function of cardiomyocytes is essential for the study of cardiac physiology, development, and disease, as well as the development of therapies for cardiovascular conditions.
Researchers often utilize advanced techniques, such as cell culture, electrophysiology, and genetic manipulation, to gain insights into the structure, metabolism, and role of cardiomyocytes in heart function and disease.
Techniques like FBS (fetal bovine serum), DMEM (Dulbecco's Modified Eagle Medium), Lipofectamine 2000, Penicillin/Streptomycin, TRIzol reagent, Collagenase type II, and In Situ Cell Death Detection Kit are commonly used in cardiomyocyte research to support cell growth, transfection, and analysis.
Additionally, Penicillin and Streptomycin are commonly used antibiotics to prevent bacterial contamination, while Trypsin is employed for cell detachment and passaging.
By leveraging these advanced tools and techniques, researchers can optimize their myocyte and cardiac studies, enhance reproducibility and accuracy, and drive progress in the understanding and treatment of cardiovascular conditions.