Log In Sign up
The largest database of trusted experimental protocols
> Anatomy > Cell > Spermatocytes

Spermatocytes

Spermatocytes are specialized cells found in the testis that undergo meiotic division to produce haploid spermatids.
These cells play a crucial role in male gametogenesis, the process of sperm cell formation.
Spermatocytes are classified into primary and secondary types, each with distinct stages of development.
Understanding the biology and function of spermatocytes is essential for researchers investigating male fertility, reproductive disorders, and the underlying mechanisms of spermatogenesis.
PubCompare.ai's AI-driven platform can optimize your spermatocytes research by helping you effortlessly locate relevant protocols from literature, pre-prints, and patents, and identify the most accurate and reproducilbe methods to enhance the quality of your work.

Most cited protocols related to «Spermatocytes»

1
Identifying Human Meiotic DSB Hotspots
We performed SSDS (27 (link)) using antibodies against DMC1 (Santa Cruz; C-20, sc 8973), to identify meiotic DSB hotspots in testis tissue from five individual human males. The genotype at the PRDM9 locus was established in each of these individuals using the primers and method described in (9 (link)). SSDS samples were compared to a matched control and DSB hotspots were defined using MACS 2.0.10 (59 (link)). Whole genome sequencing libraries were prepared for three individuals according to an established protocol (Illumina). GATK best practices (60 ) were followed to identify variants using the GATK Genome Analysis Toolkit v2.3.9 (61 (link)). Chromatin immunoprecipitation using antibodies against H3K4me3 (Abcam; ab8580) followed by high throughput sequencing was performed to identify sites of trimethylated H3K4 in one individual. Peaks in H3K4me3 data were called using SICER v1.1 (62 (link)). All high throughput sequencing was performed on an Illumina HiSeq 2500. Spermatocyte spreads were prepared for immunofluorescence microscopy using the method described in (63 (link)). Detailed methods are available in Supplementary Materials.
Pratto F., Brick K., Khil P., Smagulova F., Petukhova G.V, & Camerini-Otero R.D. (2014). Recombination initiation maps of individual human genomes. Science (New York, N.Y.), 346(6211), 1256442.
Publication 2014
Antibodies DMC1 protein, human Genome Genotype histone H3 trimethyl Lys4 Immunofluorescence Microscopy Immunoprecipitation, Chromatin Males Miotics Oligonucleotide Primers Spermatocytes SSD Testis Tissues
2
Genome-Wide Recombination Mapping in Village Dogs
DNA sequencing of 51 village dogs was performed using Illumina technology to 8–12 fold coverage, using 101 base-pair paired end reads (Table S1 and Table S2). Reads were aligned to the reference genome using bwa[31] (link). Variant calls were made using GATK[32] (link), and phased using BEAGLE[33] (link). Extensive filters were applied to ensure that only high quality variants were used for the purposes of recombination rate estimation (see Supplementary Text S1 for details). After filtering, recombination rates were estimated using the statistical package, LDhat[15] (link).
For H3K4me3 ChIPseq experiments, spermatocytes of various stages were cell types were purified sedimentation velocity (STA-PUT) of collagenase digested single cell suspensions. Chromatin immunoprecipitation (ChIP) of H3K4me3 was performed using standard procedures, and validated through qPCR (Table S6). Libraries were prepared using Tru-Seq adaptors, with sequencing performed using 150 bp paired-end reads from an Illumina HiSeq 2500 in Rapid Run mode. Reads were mapped to the canine reference genome, and H3K4me3 peaks called using MACS[34] (link).
Detailed methods are available in the Supplementary Information. Genetic maps and called hotspots are available for download from: http://autonlab.einstein.yu.edu/dog_recomb/
Auton A., Rui Li Y., Kidd J., Oliveira K., Nadel J., Holloway J.K., Hayward J.J., Cohen P.E., Greally J.M., Wang J., Bustamante C.D, & Boyko A.R. (2013). Genetic Recombination Is Targeted towards Gene Promoter Regions in Dogs. PLoS Genetics, 9(12), e1003984.
Publication 2013
Base Pairing Cells Chromosome Mapping Collagenase Genome histone H3 trimethyl Lys4 Immunoprecipitation, Chromatin Recombination, Genetic Spermatocytes
3
Whole-Mount Spermatocyte Chromosome Spread Protocol
The standard chromosome spreading protocol has been described previously for our laboratory (Lenzi et al., 2005 (link)). The nuclear contents of whole-mount spermatocytes (or oocytes) were displayed by drying down a cell suspension, in hypotonic buffer, from either testis, or ovary, in 1% paraformaldehyde containing 0.15% Triton X-100 (Peters et al., 1997 (link)). Whole testes or ovaries were incubated on ice for 60 min in hypotonic extraction buffer (HEB; 30 mM Tris, pH 8.2, 50 mM sucrose, 17 mM trisodium citrate dihydrate, 5 mM EDTA, 0.5 mM DTT, and 0.5 mM PMSF). Either a one-inch length of tubule, or a whole ovary, were placed in a 20-μl drop of 100 mM sucrose, pH 8.2, the tissue was macerated, and a second 20-μl drop of sucrose solution was added and the cell suspension was pipetted up and down several times. Remnant pieces of tubule were removed. Cleaned slides were dipped in the paraformaldehyde and Triton X-100 solution, and most liquid was drained off, such that only enough liquid remained to coat the slide. 20 μl of the cell suspension was added in one corner and the cells were slowly dispersed, first in a horizontal direction and then vertical. The remaining 20 μl of cell suspension was used to make a second slide and both were placed in a humid chamber to dry slowly at RT for 2 h. The slides were washed three times for 1 min in 0.4% Kodak Photo-Flo 200 and air dried for at least 15 min. For EM preparations, to make the SCs accessible to immunogold grains, the slides were DNaseI treated (1 μl/ml of DMEM) before being air dried (Moens et al., 2002 (link)). The slides were washed and blocked (three times for 10 min each) in PBS and incubated in primary antibodies overnight at RT in a humid chamber. Primary antibodies were used at varying concentrations, and generally a 10-fold higher concentration was used for EM than immunofluorescence. After washes, slides were incubated in secondary antibodies, conjugated to either fluorochrome or colloidal gold (Jackson ImmunoResearch Laboratories), for 2 h at 37°C. After washes the slides were mounted with ProLong Antifade (Invitrogen) for fluorescence microscopy. Images were captured on a Olympus IX81 microscope attached to a 12-bit Cooke Sensicam CCD instrument and sent to IP Lab software.
For EM, slides were incubated in 4% alcoholic phosphotungstic acid for 15 min, followed by three 1-min washes in 95% ethanol, to enhance visualization of MNs. Slides were air dried and then dipped in 0.25% formvar (Electron Microscopy Sciences) and air dried under glass. The plastic was scored, treated with 25% hydrofluoric acid, and floated off in water with attached cells. Plastic was transferred to EM grids and used for transmission EM (JEOL 1200EX).
Kolas N.K., Svetlanov A., Lenzi M.L., Macaluso F.P., Lipkin S.M., Liskay R.M., Greally J., Edelmann W, & Cohen P.E. (2005). Localization of MMR proteins on meiotic chromosomes in mice indicates distinct functions during prophase I. The Journal of Cell Biology, 171(3), 447-458.
Publication 2005
Alcoholics Antibodies Buffers Cells Cereals Chromosomes Edetic Acid Electron Microscopy Ethanol Fluorescent Antibody Technique Fluorescent Dyes Formvar Gold Colloid Hydrofluoric acid Microscopy Microscopy, Fluorescence Oocytes Ovary paraform Phosphotungstic Acid Sodium Citrate Dihydrate Spermatocytes Sucrose Testis Tissues Transmission, Communicable Disease Triton X-100 Tromethamine
4
Histological Analysis of Meiotic Progression
For histology, testes were fixed in Bouin's solution, embedded in paraffin, sectioned, and stained with hematoxylin and eosin. Surface spread of spermatocyte nuclei was performed as previously described (Peters et al., 1997 (link); Kolas et al., 2005). To obtain fetal oocytes, Sycp2−/− females were mated with Sycp2+/− males. Vaginal copulatory plugs were checked the next morning. Fetal oocytes were collected for analysis at 17.5 dpc. The primary antibodies used for immunofluorescence were as follows: anti-SYCP1 (a gift from P. Moens and B. Spyropoulos [York University, Toronto, Ontario, Canada] and C. Höög [Karolinska Institute, Stockholm, Sweden]; Dobson et al., 1994 (link); Liu et al., 1996 (link); Schmekel et al., 1996 (link)), anti-SYCP2 serum 493 (1:400; a gift from C. Heyting, Wageningen University, Wageningen, Netherlands; Offenberg et al., 1998 (link)), anti-SYCP3 (1:500; a gift from S. Chuma, Kyoto University, Kyoto, Japan; Chuma and Nakatsuji, 2001 (link)), anti-STAG3 (1:500; a gift from J.L. Barbero, Centro Nacional de Biotecnologia, Madrid, Spain; Prieto et al., 2001 (link)), FITC-conjugated anti-γH2AX (1:500; Upstate Biotechnology), and CREST antiserum (1:5,000; a gift from B.R. Brinkley, Baylor College of Medicine, Houston, TX). Tissue sections were visualized under an Axioskop 40 microscope. Images were captured with a digital camera (Evolution QEi; MediaCybernetics) and processed with ImagePro software (Phase 3 Imaging Systems) and Photoshop (Adobe).
Yang F., Fuente R.D., Leu N.A., Baumann C., McLaughlin K.J, & Wang P.J. (2006). Mouse SYCP2 is required for synaptonemal complex assembly and chromosomal synapsis during male meiosis. The Journal of Cell Biology, 173(4), 497-507.
Publication 2006
Antibodies Biological Evolution Bouin's solution Care, Prenatal Cell Nucleus Cola Crista Ampullaris Eosin Females Fingers Fluorescein-5-isothiocyanate Fluorescent Antibody Technique Immune Sera Males Microscopy Ovum Paraffin Embedding Pharmaceutical Preparations Serum Spermatocytes Testis Tissues Vagina
5
Zebrafish Testis Gene Expression Analysis
Testes from males kept at 27°C (control) (n = 7), or at 35°C (n = 5), or treated with busulfan (n = 12) were snap frozen in liquid nitrogen and stored at −80°C until RNA extraction. Total RNA was extracted from testes using the RNAqueous®-Micro Kit (Ambion, Austin, TX, USA, http://www.ambion.com). Further processing to determine the threshold cycle (Cq) values of the reference endogenous control gene elongation factor 1-alpha (ef1α) and β-actin1, as well as of insulin-like 3 (insl3)[35] (link), steroidogenic acute regulatory protein (star), and cytochrome P450, family 17, subfamily A, polypeptide 1 (cyp17a1), androgen receptor (ar), anti-Müllerian hormone (amh), gonadal soma-derived growth factor (gsdf), insulin growth factor 1a (igf1a) and 1b (igf1b)[36] (link), and germ cell genes piwil1 (spermatogonia), and synaptonemal complex protein 3 (sycp3l) (spermatocytes) by qPCR analysis was performed as reported [33] (link), [37] (link), [38] (link). No significant differences (P>0.05) were found among the mean β-actin1 and ef1α Cq values in the different groups (Figure S3) thus validating β-actin1 and ef1α as suitable references for the current experiments. Then, relative mRNA levels of the selected genes were normalized to β-actin1 and ef1α, and expressed as fold of relative control (27°C) mRNA levels. Nomenclature of zebrafish proteins, mRNAs and genes are according to ZFIN (http://zfin.org) rules.
Nóbrega R.H., Greebe C.D., van de Kant H., Bogerd J., de França L.R, & Schulz R.W. (2010). Spermatogonial Stem Cell Niche and Spermatogonial Stem Cell Transplantation in Zebrafish. PLoS ONE, 5(9), e12808.
Publication 2010
AR protein, human austin Busulfan Carisoprodol CYP17A1 protein, human Cytochrome P450 Elongation Factor 1alpha Freezing Genes Germ Cells Growth Factor Insulin Males Mullerian-Inhibiting Hormone Nitrogen Polypeptides Proteins RNA, Messenger Spermatocytes Spermatogonia steroidogenic acute regulatory protein Synaptonemal Complex Testis Zebrafish Proteins

Most recents protocols related to «Spermatocytes»

1
Generation of Tamalin and Cytip Knockout Mice
Tamalin KO mice were kindly provided by Dr. Lino Tessarollo [58 (link)]. We obtained mice harboring the Cytip tm1a “knockout first” allele from the Mutant Mouse Resource and Research Centers (MMRRC) at University of California-Davis. The Cytip tm1a allele has loxP sites flanking exon 4 and 5. Mice heterozygous for Cytip tm1a were bred with mice harboring the Spo11-Cre transgene (C57BL/6-Tg Spo11-cre)Rsw/PecoJ), which express Cre recombinase in spermatocytes shortly after meiotic entry [77 (link)]https://sciwheel.com/work/citation?ids=1181041&pre=&suf=&sa=0&dbf=0. The resulting progeny from this cross harbored the Cytip tm1b KO allele. We subsequently bred mice harboring the Tamalin KO and Cytip KO alleles to create the Tamalin, Cytip DKO mice for analysis. We also bred mice heterozygous for Cytip tm1a allele with mice harboring FLP recombinase transgene (FLP tg/0) to produce progeny with the Cytip tm1c “conditional knockout” (cKO) allele. These mice were used to create the Cytip cKO mice that were homozygous for the Cytip tm1c allele and hemizygous for the Spo11-Cre transgene.
Martinez-Garcia M., Naharro P.R., Skinner M.W., Baran K.A., Lascarez-Lagunas L.I., Nadarajan S., Shin N., Silva-García C.G., Saito T.T., Beese-Sims S., Diaz-Pacheco B.N., Berson E., Castañer A.B., Pacheco S., Martinez-Perez E., Jordan P.W, & Colaiácovo M.P. (2023). GRAS-1 is a novel regulator of early meiotic chromosome dynamics in C. elegans. PLOS Genetics, 19(2), e1010666.
Publication 2023
Alleles Cre recombinase Exons FLP recombinase GRASP protein, human Hemizygote Heterozygote Homozygote Mice, Knockout Mice, Laboratory Miotics Spermatocytes SPO11 protein, human Transgenes
2
Chromatin Spread Preparation and Staining
Spermatocyte and oocyte chromatin spreads were prepared as previously described [78 (link)–80 (link)]. Primary antibodies and dilution used for immunolabeling are presented in S4 Table. Secondary antibodies against human, rabbit, rat, mouse, and guinea pig IgG and conjugated to Alexa 350, 488, 568, or 633 (Life Technologies) were used at a 1:500 dilution.
Images from chromatin spread preparations were captured using a Zeiss CellObserver Z1 microscope linked to an ORCA-Flash 4.0 CMOS camera (Hamamatsu). Testis sections stained with H&E staining were captured using a Zeiss AxioImager A2 microscope linked to an AxioCam ERc5s camera, or Keyence BZ-X800 fluorescence microscope. Images were analyzed and processed using ZEN 2012 blue edition imaging software (Zeiss) or with BZ-X800 Viewer and Analyzer software (Keyence).
Martinez-Garcia M., Naharro P.R., Skinner M.W., Baran K.A., Lascarez-Lagunas L.I., Nadarajan S., Shin N., Silva-García C.G., Saito T.T., Beese-Sims S., Diaz-Pacheco B.N., Berson E., Castañer A.B., Pacheco S., Martinez-Perez E., Jordan P.W, & Colaiácovo M.P. (2023). GRAS-1 is a novel regulator of early meiotic chromosome dynamics in C. elegans. PLOS Genetics, 19(2), e1010666.
Publication 2023
Alexa 350 Antibodies Cavia Chromatin Chronic multifocal osteomyelitis Homo sapiens Mice, House Microscopy Microscopy, Fluorescence Oocytes Orcinus orca Rabbits Spermatocytes Technique, Dilution Testis
3
Evaluation of Testicular Histopathology
The sections were evaluated according to the following morphological criteria [14 (link)]:

Absence of seminiferous tubules (tubular sclerosis);

Absence of germ cells (Sertoli cell only syndrome);

Maturation arrest – spermatogenesis arrested at different stages (spermatogonia, spermatocytes, or spermatids);

Hypospermatogenesis – all cell types up to spermatozoa are present, but there is a distinct decline in reproducing spermatogonia;

Mixed atrophy can be global (present in all tubules) or focal, with a variable percentage of tubules displaying various stages of qualitatively and quantitatively limited spermatogenesis.

Spermatogenesis was assessed using Johnsen's score. The Johnsen criteria convert the cell profile found along the seminiferous tubules into a ten-point score system for assessing spermatogenesis. Johnsen scores range from 1 to 10, with 1 representing the complete absence of germ cells and 10 representing maximum spermatogenesis activity.
Arian I., Dumbraveanu I., Ghenciu V., Machidon D., Ernu I, & Ceban E. (2023). Histological and immunohistochemical outcomes after microdissection TESE in contrast with hormonal profile, testis volume and genetics in patients with azoospermia. Journal of Medicine and Life, 16(1), 144-152.
Publication 2023
Atrophy Cells Germ Cells Oligospermia Sclerosis Seminiferous Tubule Sertoli Cell-Only Syndrome Sperm Spermatid Spermatocytes Spermatogenesis Spermatogonia
4
Testicular Biopsy in Pediatric Cancers
Patient data were identified through AUMC and UZB records, including age at time of testicular biopsy, testicular volume by ultrasound, SD of height- and weight-for-age z-score (WHO, 2006 ), general health condition, potential pre-treatment, and disease diagnosis. Based on diagnoses, we classified patients into four groups: extra-cerebral cancer (solid tumors), central nervous system cancer (CNS tumors), leukemia and lymphoma (blood cancer), and non-malignant hematological disorders.
We analyzed the patient data in the age groups 0 to <4, 4 to <7, 7 to <11, and 11 to <14 years, to reflect the prepubertal testicular and germ cell developmental periods, namely, gonocyte differentiation into spermatogonia and dispersion throughout elongating seminiferous tubules; proliferation of spermatogonia to populate the testes; gradual maturation of somatic testicular environment; and finally Sertoli cell maturation allowing for spermatogonial differentiation into spermatocytes and later spermatids, respectively (Masliukaite et al., 2016 (link)).
Masliukaite I., Ntemou E., Feijen E.A., van de Wetering M., Meissner A., Soufan A.T., Repping S., Kremer L.M., Jahnukainen K., Goossens E, & van Pelt A.M. (2023). Childhood cancer and hematological disorders negatively affect spermatogonial quantity at diagnosis: a retrospective study of a male fertility preservation cohort. Human Reproduction (Oxford, England), 38(3), 359-370.
Publication 2023
Age Groups Biopsy Central Nervous System Neoplasms Diagnosis Diploid Cell Germ Cells Hematological Disease Hematologic Neoplasms Leukemia Lymphoma Malignant Neoplasms Neoplasms Nervous System Neoplasms Patients Seminiferous Tubule Sertoli Cells Spermatid Spermatocytes Spermatogonia Ultrasonography
5
Generation and Characterization of Angptl2 and Mag Knockout Mice
The Angptl2 knockout mice (Angptl2fl/fl) with insertion of loxp in flanks of exon 2 in C57BL/6 background were generated by Nanjing Mouse Model, Lt Corporation. The male Angptl2fl/fl; stra8-cre mice (Angptl2 is specifically deleted in early-stage spermatogonia, spermatocytes and sperm) were crossed with wild-type (WT) female mice to generate Angptl2 knockout mice (Angptl2−/−). The genetic deletion of Angptl2 was further confirmed by PCR. The Mag knockout mice (Mag−/−) in C57BL/6 background were purchased from Mutant Mouse Regional Resource Centers (MMRRC). Mag−/−Angptl2−/− double knockout mice and their littermates Mag−/−Angptl2+/+ mice were also bred for the related experiments. The Guideline for Animal Care at Shanghai Jiao Tong University School of Medicine approved all the animal experimental procedures.
Chen L., Yu Z., Xie L., He X., Mu X., Chen C., Yang W., Tong X., Liu J., Gao Z., Sun S., Xu N., Lu Z., Zheng J, & Zhang Y. (2023). ANGPTL2 binds MAG to efficiently enhance oligodendrocyte differentiation. Cell & Bioscience, 13, 42.
Publication 2023
Animals Exons Females Gene Deletion Males Mice, Knockout Mice, Laboratory Sperm Spermatocytes Spermatogonia

Top products related to «Spermatocytes»

1
Trizol reagent by Thermo Fisher Scientific
Sourced in United States, China, Japan, Germany, United Kingdom, Canada, France, Italy, Australia, Spain, Switzerland, Netherlands, Belgium, Lithuania, Denmark, Singapore, New Zealand, India, Brazil, Argentina, Sweden, Norway, Austria, Poland, Finland, Israel, Hong Kong, Cameroon, Sao Tome and Principe, Macao, Taiwan, Province of China, Thailand
TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA, DNA, and proteins from a variety of biological samples. The reagent maintains the integrity of the RNA while disrupting cells and dissolving cell components.
2
Photo flo by Kodak
Sourced in United States
Photo-Flo is a product designed to facilitate the drying of photographic films and papers. It is a wetting agent that helps to prevent water spots and uneven drying. The core function of Photo-Flo is to improve the drying process of photographic materials.
3
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, France, Australia, Canada, Japan, Italy, Switzerland, Belgium, Austria, Spain, Israel, New Zealand, Ireland, Denmark, India, Poland, Sweden, Argentina, Netherlands, Brazil, Macao, Singapore, Sao Tome and Principe, Cameroon, Hong Kong, Portugal, Morocco, Hungary, Finland, Puerto Rico, Holy See (Vatican City State), Gabon, Bulgaria, Norway, Jamaica
DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
4
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
5
Hiseq 2500 by Illumina
Sourced in United States, China, Germany, United Kingdom, Canada, Switzerland, Sweden, Japan, Australia, France, India, Hong Kong, Spain, Cameroon, Austria, Denmark, Italy, Singapore, Brazil, Finland, Norway, Netherlands, Belgium, Israel
The HiSeq 2500 is a high-throughput DNA sequencing system designed for a wide range of applications, including whole-genome sequencing, targeted sequencing, and transcriptome analysis. The system utilizes Illumina's proprietary sequencing-by-synthesis technology to generate high-quality sequencing data with speed and accuracy.
6
In situ cell death detection kit by Roche
Sourced in Germany, United States, Switzerland, China, United Kingdom, France, Canada, Belgium, Japan, Italy, Spain, Hungary, Australia
The In Situ Cell Death Detection Kit is a laboratory product designed for the detection of programmed cell death, or apoptosis, in cell samples. The kit utilizes a terminal deoxynucleotidyl transferase (TdT) to label DNA strand breaks, allowing for the visualization and quantification of cell death. The core function of this product is to provide researchers with a tool to study and analyze cell death processes.
7
Hoechst 33342 by Thermo Fisher Scientific
Sourced in United States, Germany, United Kingdom, Japan, China, France, Canada, Spain, Belgium, Italy, Australia, Austria, Denmark, Netherlands, Switzerland, Ireland, New Zealand, Portugal, Brazil, Argentina, Singapore, Poland, Ukraine, Macao, Thailand, Finland, Lithuania, Sweden
Hoechst 33342 is a fluorescent dye that binds to DNA. It is commonly used in various applications, such as cell staining and flow cytometry, to identify and analyze cell populations.
8
Ab97672 by Abcam
Sourced in United States, United Kingdom
Ab97672 is a lab equipment product. It is a high-quality tool designed for use in scientific research applications. The core function of this product is to provide a reliable and efficient solution for laboratory procedures.
9
Ab15090 by Abcam
Sourced in United States
Ab15090 is a mouse monoclonal anti-GAPDH antibody. GAPDH is a key enzyme involved in glycolysis and is commonly used as a loading control for western blotting.
10
Agilent 2100 bioanalyzer by Agilent Technologies
Sourced in United States, Germany, Canada, China, France, United Kingdom, Japan, Netherlands, Italy, Spain, Australia, Belgium, Denmark, Switzerland, Singapore, Sweden, Ireland, Lithuania, Austria, Poland, Morocco, Hong Kong, India
The Agilent 2100 Bioanalyzer is a lab instrument that provides automated analysis of DNA, RNA, and protein samples. It uses microfluidic technology to separate and detect these biomolecules with high sensitivity and resolution.

More about "Spermatocytes"

Spermatogenesis, Male Gamete Formation, Meiotic Division, Gametogenesis, Reproductive Biology, Testicular Cells, Sperm Production, Primary Spermatocytes, Secondary Spermatocytes, Spermatid Development, Fertility Research, Reproductive Disorders, Testis Function, TRIzol Reagent, Photo-Flu, DMEM, FBS, HiSeq 2500, In Situ Cell Death Detection Kit, Hoechst 33342, Ab97672, Ab15090, Agilent 2100 Bioanalyzer.
Spermatocytes are the specialized cells found in the testis that undergo meiotic division to produce haploid spermatids, which are the precursors to mature sperm cells.
These cells play a crucial role in male gametogenesis, the process of sperm cell formation.
Spermatocytes are classified into primary and secondary types, each with distinct stages of development.
Understanding the biology and function of spermatocytes is essential for researchers investigating male fertility, reproductive disorders, and the underlying mechanisms of spermatogenesis.
PubCompare.ai's AI-driven platform can optimize your spermatocytes research by helping you effortlessly locate relevant protocols from literature, pre-prints, and patents, and identify the most accurate and reproducible methods to enhance the quality of your work.
Leverage TRIzol reagent, Photo-Flo, DMEM, FBS, HiSeq 2500, In Situ Cell Death Detection Kit, Hoechst 33342, Ab97672, Ab15090, and Agilent 2100 Bioanalyzer to further advance your spermatocytes research and gain deeper insights into this critical aspect of male reproductive biology.