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> Anatomy > Embryonic Structure > Umbilical Vein

Umbilical Vein

The umbilical vein is a blood vessel that carries oxygenated blood from the placenta to the fetus during pregnancy.
It plays a crucial role in fetal development by facilitating the exchange of nutrients, oxygen, and waste products between the mother and child.
The umbilical vein is an important structure for researchers studying prenatal physiology and the factors that influence fetal growth and well-being.
Optimizing protocols for analyzing the umbilical vein can enhance the reproducibility and accuracy of research in this field, leading to more reliable results that advance our understanding of this vital component of the fetal circulatory system.

Most cited protocols related to «Umbilical Vein»

1
Comprehensive RNA-seq Data Analysis
Publicly available Illumina RNA-seq data sets of D. melanogaster [SRA:SRR166809] and a human melanoma sample [SRA:SRR018261-62] were downloaded from the National Center for Biotechnology Information short read archive. The 454 sequencing data for a human umbilical vein RNA-seq experiment [27 (link)] and RNA capture sequencing experiments of human fibroblasts [24 (link)] were retrieved from the Gene Expression Omnibus under [GEO:GSM951482] and [GEO:GSE29040]. The RNA-seq sample for HEK293 cells investigated in [5 (link)] was retrieved under [GEO:GSE43574]. Finally, we applied our algorithm to a C. elegans data set [SRA:SRX151602] to investigate RNA leader trans-splicing. All of these data sets are non-strand specific. In addition, we analyzed a strand-specific prostate cancer data set (see Additional file 1).
Hoffmann S., Otto C., Doose G., Tanzer A., Langenberger D., Christ S., Kunz M., Holdt L.M., Teupser D., Hackermüller J, & Stadler P.F. (2014). A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection. Genome Biology, 15(2), R34.
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Publication 2014
Fibroblasts Gene Expression HEK293 Cells Homo sapiens Melanoma Prostate Cancer RNA-Seq Umbilical Vein
2
Cell Culture Conditions for Cancer Lines
The human lines colon adenocarcinoma (HT29), colorectal carcinoma (HCT116), human fibroblast (HF), endothelial umbilical vein (E926), and sarcoma (SW872 and SW982, provided by ATCC) were all cultured in DMEM (Dulbecco’s modified Eagle’s medium, Eurobio, Les Ulis, France), supplemented with 10% heat-inactivated FBS (Gibco, Life technologies, Milan, Italy), 1% penicillin/streptomycin and 1% Glutammine (Gibco, Life technologies, Milan, Italy). The myxoid sarcoma lines 402–91 WT [22 (link)] and the resistant counterpart 402–91 ET [23 (link)] were maintained in RPMI medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin (Gibco, Life technologies, Milan, Italy). All lines grow at 37 °C in a humidified atmosphere with 5% CO2.
Loria R., Giliberti C., Bedini A., Palomba R., Caracciolo G., Ceci P., Falvo E., Marconi R., Falcioni R., Bossi G, & Strigari L. (2019). Very low intensity ultrasounds as a new strategy to improve selective delivery of nanoparticles-complexes in cancer cells. Journal of Experimental & Clinical Cancer Research : CR, 38, 1.
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Publication 2019
Atmosphere Colon Adenocarcinomas Colorectal Carcinoma Eagle Endothelium Fibroblasts Homo sapiens HT29 Cells Penicillins Sarcoma Streptomycin Umbilical Vein
3
Isolation and Expansion of Umbilical Cord Blood-Derived Stromal Cells
CB was collected from the umbilical cord vein with informed consent of the mother (21 (link)). USSCs were generated from 94 (40.3%) of 233 CB. The mononuclear cell fraction was obtained by Ficoll (Biochrom) gradient separation followed by ammonium chloride lysis of RBCs. Cells were plated out at 5–7 × 106 cells/ml in T25 culture flasks (Costar). Two different media were used to initiate growth of the adherent USSC colonies: myelocult medium (StemCell Technologies) and low glucose DMEM (Cambrex) with 30% FCS, dexamethasone (10−7 M; Sigma-Aldrich), penicillin (100 U/ml; Grünenthal), streptomycin (0.1 mg/ml; Hefa-pharma), and ultraglutamine (2 mM; Cambrex). Expansion of the cells was performed in the same media but with a lower concentration or in the absence of dexamethasone. Cells were incubated at 37°C in 5% CO2 in a humidified atmosphere. When cells reached 80% confluency, they were detached with 0.25% trypsin (Cambrex) and replated 1:3 as outlined in Table S1 available at http://www.jem.org/cgi/content/full/jem.20040440/DC1. USSC karyotyping was performed by cytogenetic standard protocols.
Kögler G., Sensken S., Airey J.A., Trapp T., Müschen M., Feldhahn N., Liedtke S., Sorg R.V., Fischer J., Rosenbaum C., Greschat S., Knipper A., Bender J., Degistirici Ö., Gao J., Caplan A.I., Colletti E.J., Almeida-Porada G., Müller H.W., Zanjani E, & Wernet P. (2004). A New Human Somatic Stem Cell from Placental Cord Blood with Intrinsic Pluripotent Differentiation Potential. The Journal of Experimental Medicine, 200(2), 123-135.
Publication 2004
Atmosphere Cells Chloride, Ammonium Cone-Rod Dystrophy 2 Dexamethasone Erythrocytes Ficoll Glucose Mothers Penicillins Stem Cells Streptomycin Trypsin Umbilical Cord Umbilical Vein Veins
4
Identifying IL1B-Responsive Gene Sets
In addition to our validation experiment (GSE59671), we curated all series in GEO that included treatment of cells with IL1B and controls. This resulted in nine datasets: GSE13168 (airway smooth muscle), GSE26315 (amnion mesenchymal cells), GSE31679 (trophoblast cells), GSE40007 (endometrial stromal cells), GSE49604 osteoarthritis, GSE7216 (keratinocytes), GSE37624 (umbilical vein endothelial cells), GSE40560 (fibroblasts), and GSE40838 (peripheral blood mononuclear cells). Of these datasets, only GSE7216 was included in the data compendium used for integration. The rest were independent of the integration. To assess our networks’ ability to identify gene sets that would respond to IL1B treatment, we contrasted IL1B treatments with controls using GEO2R10 . We queried the GIANT webserver for neighbors of IL1B in the tissue network that best corresponded to each dataset. In each tissue, genes were ranked based on the connectivity measure described above. We evaluated the mean fold change of the top twenty returned results. We evaluated randomly selected matched size sets of genes from each dataset as controls.
Greene C.S., Krishnan A., Wong A.K., Ricciotti E., Zelaya R.A., Himmelstein D.S., Zhang R., Hartmann B.M., Zaslavsky E., Sealfon S.C., Chasman D.I., FitzGerald G.A., Dolinski K., Grosser T, & Troyanskaya O.G. (2015). Understanding multicellular function and disease with human tissue-specific networks. Nature genetics, 47(6), 569-576.
Publication 2015
Amnion Cells Degenerative Arthritides Endometrium Endothelial Cells Fibroblasts Genes Gigantism Interleukin-1 Keratinocyte Mesenchymal Stem Cells PBMC Peripheral Blood Mononuclear Cells Smooth Muscles Stromal Cells Tissues Trophoblast Umbilical Vein
5
Endothelial Cell Flow Mechanics and Gene Silencing
Human umbilical vein EC and porcine aortic EC were isolated and cultured.32 (link) Gene silencing was performed using 2 different small interfering RNAs (siRNAs) against TWIST1 ((Silencer Select S14523, Ambion, and L-006434-00-0005 ON-TARGETplus SMARTpool; Dharmacon) or GATA4 (Silencer Select s5603, Ambion, and L-008244-00-0005 ON-TARGETplus SMARTpool; Dharmacon). They were exposed to flow using an orbital shaking system or Ibidi parallel-plate system.32 (link),33 (link) Quantitative RT-PCR, immunofluorescent staining and chromatin immunoprecipitation,6 (link) and assays of permeability34 (link) were performed as described.
Mahmoud M.M., Kim H.R., Xing R., Hsiao S., Mammoto A., Chen J., Serbanovic-Canic J., Feng S., Bowden N.P., Maguire R., Ariaans M., Francis S.E., Weinberg P.D., van der Heiden K., Jones E.A., Chico T.J., Ridger V, & Evans P.C. (2016). TWIST1 Integrates Endothelial Responses to Flow in Vascular Dysfunction and Atherosclerosis. Circulation Research, 119(3), 450-462.
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Publication 2016
Aorta Biological Assay Homo sapiens Immunofluorescence Immunoprecipitation, Chromatin Pigs Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering TWIST1 protein, human Umbilical Vein

Most recents protocols related to «Umbilical Vein»

1
Evaluating CTGF Inhibition on HUVEC Viability
The viability of human umbilical vein endothelial cells (HUVECs) in 96-well plate was measured by CCK-8 assay system (CK04, Dojindo). Briefly, HUVECs were plated on 96-well plate (5 × 103 per well), treated with CTGF (50 nM) and with or without anti-CTGF scFvs (1-500 nM). After 8 h intervention, 10 µL CCK-8 solution was added to each well and incubated for 1 h in 37 ℃. The absorbance was measured at 450 nm. The cell proliferation rate was calculated using the following formula: [Absorbance (8 h) – Absorbance (control)] / [Absorbance (0 h) – Absorbance (control)] × 100%, absorbance of well that only contains culture medium and CCK-8 kit was used as Absorbance (control). Inhibition rate was calculated using the following formula: [(cell proliferation rate of only CTGF-treated HUVECs - cell proliferation rate of scFv-treated HUVECs) / (cell proliferation rate of CTGF-treated HUVECs - cell proliferation rate of untreated HUVECs)] ×100%. The experiments were repeated in triplicate.
Qin Y., Wu G., Jin J., Wang H., Zhang J., Liu L., Zhao H., Wang J, & Yang X. (2023). A fully human connective tissue growth factor blocking monoclonal antibody ameliorates experimental rheumatoid arthritis through inhibiting angiogenesis. BMC Biotechnology, 23, 6.
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Publication 2023
Biological Assay Cells Cell Survival Connective Tissue Growth Factor Culture Media Endothelium Homo sapiens Human Umbilical Vein Endothelial Cells Psychological Inhibition Sincalide Umbilical Vein
2
Modulating HUVEC Angiogenic Response to VEGF
Human umbilical vein ECs (HUVECs, ATCC, Manassas) were seeded in confluent conditions (8 × 105 cells/well) in 6-well plates. HUVECs cultured in Human Endothelial Medium (LL-0003, Lifeline Cell Technology, Frederick) for 24 h were pretreated with 100 ng/ml human angiopoietin-1 (ANGPT1, 923-AN/CF, R&D Systems, Minneapolis), 100 ng/ml angiopoietin-2 (ANGPT2, 623-AN/CF, R&D Systems), 30 μg/mL 4E2, or 2.5 μM AKB-9778 for 20 min and were then treated with 100 ng/mL human VEGF-165 protein (293-VE/CF, R&D Systems) for 10 min. When indicated, CHO-K1 cells expressing human Tie2 or mouse Tie2 were maintained in DMEM (Thermo Fisher, Waltham) supplemented with 10% FBS and 200 ng/ml hygromycin (Thermo Fisher).
Lee E., Lee E.A., Kong E., Chon H., Llaiqui-Condori M., Park C.H., Park B.Y., Kang N.R., Yoo J.S., Lee H.S., Kim H.S., Park S.H., Choi S.W., Vestweber D., Lee J.H., Kim P., Lee W.S, & Kim I. (2023). An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone. Experimental & Molecular Medicine, 55(2), 470-484.
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Publication 2023
AKB-9778 Angiopoietin-2 ANGPT1 protein, human ANGPT2 protein, human Cells CHO Cells Culture Media Endothelium Homo sapiens hygromycin A Mus Umbilical Vein VEGF165 protein, human
3
Quantifying Angiogenic Sprout Formation
Fibrin-bead assay was performed as reported by Nakatsu et al.[28 (link), 29 (link)]. Briefly, human umbilical vein ECs were coated onto microcarrier beads and plated overnight. SiRNA-treatment or viral transduction was performed the same day the beads were coated. The following day, the EC-covered microbeads were embedded in a fibrin matrix. Once a clot was formed, media was overlaid along with approximately 100,000 normal human lung fibroblasts. Media was changed daily along with monitoring of sprout development. Sprout characteristics were quantified in the following manner. Sprout numbers were determined by counting the number of multicellular sprouts (sprouts that did not contain at least 3 cells were not counted) emanating from an individual microcarrier beads across multiple beads in each experiment. Sprout lengths were determined by measuring the length of a multicellular sprout beginning from the tip of the sprout to the microcarrier bead surface across multiple beads. Percent of non-lumenized sprouts were determined by quantifying the proportion of multicellular sprouts whose length (microcarrier bead surface to sprout tip) was <80% lumenized across multiple beads. Sprout widths were determined by measuring the sprout width at the midpoint between the tip and the microcarrier bead across multiple beads. Experimental repeats are defined as an independent experiment in which multiple cultures, containing numerous sprouting beads were quantified; this process of quantifying multiple parameters across many beads and several cultures was replicated on different days for each experimental repeat.
Francis C.R., Bell M.L., Skripnichuk M.M, & Kushner E.J. (2023). Arf6 Regulates Endocytosis and Angiogenesis by Promoting Filamentous Actin Assembly. bioRxiv.
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Publication Preprint 2023
Biological Assay Cells Clotrimazole Fibrin Fibroblasts Homo sapiens Lung Microspheres RNA, Small Interfering Umbilical Vein
4
Umbilical Vein EC Culture and Transfection
Pooled human umbilical vein ECs cultured in proprietary media (PromoCell Growth Medium, ready-to-use) for 2 to 5 passages. For experiments, glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine for a minimum of 20 minutes. Small interfering RNA was introduced into primary human umbilical vein ECs using the Neon transfection system (ThermoFisher). See Supplementary Table 5 for sources of siRNA. All siRNA were resuspended to a 20 μmol/L stock concentration and used at 0.5 μmol/L. Normal human lung fibroblasts and HEK-A were maintained in DMEM supplemented with 10% fetal bovine serum and antibiotics. Both normal human lung fibroblasts and HEKs were used up to 15 passages. All cells were maintained in a humidified incubator at 37 °C and 5% CO2.
Francis C.R., Bell M.L., Skripnichuk M.M, & Kushner E.J. (2023). Arf6 Regulates Endocytosis and Angiogenesis by Promoting Filamentous Actin Assembly. bioRxiv.
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Publication Preprint 2023
Antibiotics, Antitubercular Cells Culture Media EPHA3 protein, human Fetal Bovine Serum Fibroblasts Homo sapiens Hyperostosis, Diffuse Idiopathic Skeletal Lung Lysine Neon Poly A RNA, Small Interfering Transfection Ultraviolet Rays Umbilical Vein
5
Vancomycin Pharmacokinetics in Neonates
Whole blood samples were collected from newly inserted peripheral venous cannulas and (if available) from the central (umbilical) venous catheters. Blood samples (0.2 mL) for BDL measurement were collected at t = 0 h, t = 4 h, t = 24 h, and t = 48 h. The samples at t = 0 h were collected before the first vancomycin dose. Plasma samples (0.1 mL) were collected at t = 1 h, t = 2 h, t = 4 h, and t = 12 h for measuring vancomycin concentrations. After collection, the samples were stored at −20°C until analysis.
Additional clinical and anthropomorphic data such as dose information, GA, post-menstrual age (PMA), postnatal age (PNA), birth weight (BW), current weight (WT), length (LT), concomitant medication, C-reactive protein (CRP), and serum creatinine (SCr) were collected from the patients’ electronic medical files.
Samb A., De Kroon R., Dijkstra K., Van Den Brand M., Bos M., Van Den Dungen F., Veldkamp A., Wilhelm B., De Haan T.R., Bijleveld Y.A., Tutu Van Furth M., Savelkoul P., Swart N., Mathot R, & Van Weissenbruch M. (2023). Predicting treatment response to vancomycin using bacterial DNA load as a pharmacodynamic marker in premature and very low birth weight neonates: A population PKPD study. Frontiers in Pharmacology, 14, 1104482.
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Publication 2023
Birth Weight BLOOD Cannula Catheters C Reactive Protein Creatinine Menstruation Patients Pharmaceutical Preparations Plasma Serum Umbilical Vein Vancomycin Veins

Top products related to «Umbilical Vein»

1
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
2
Dulbecco modified eagle medium (dmem) by Thermo Fisher Scientific
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
3
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
4
Endothelial cell medium (ecm) by ScienCell
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ECM is a laboratory product that provides an extracellular matrix for cell culture applications. It serves as a substrate to support the growth and attachment of cells in vitro.
5
Streptomycin by Thermo Fisher Scientific
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
6
Heparin by Merck Group
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Heparin is a pharmaceutical product manufactured by Merck Group. It is a naturally occurring anticoagulant, primarily used as a laboratory reagent to prevent the clotting of blood samples.
7
Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
8
Egm 2 by Lonza
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The EGM-2 is a laboratory instrument designed for the measurement of oxygen concentration in cell culture media. It provides accurate and reliable data on the oxygen levels within the culture environment.
9
Ea hy926 by American Type Culture Collection
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The EA.hy926 is a cell line derived from human umbilical vein endothelial cells. It is commonly used in research as a model for endothelial cells.
10
Rpmi 1640 medium by Thermo Fisher Scientific
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.

More about "Umbilical Vein"

The umbilical vein is a crucial component of the fetal circulatory system, playing a vital role in prenatal physiology and development.
This blood vessel carries oxygenated blood from the placenta to the growing fetus, facilitating the exchange of nutrients, oxygen, and waste products between the mother and child.
Researchers studying fetal growth and well-being often focus on optimizing protocols for analyzing the umbilical vein, as this can enhance the reproducibility and accuracy of their findings.
By utilizing insights gained from the MeSH term description, researchers can better understand the significance of the umbilical vein and the factors that influence its function.
Additionally, techniques like the use of FBS (Fetal Bovine Serum), DMEM (Dulbecco's Modified Eagle Medium), and Penicillin/Streptomycin can be incorporated into umbilical vein analysis protocols to maintain cell viability and prevent contamination.
The extracellular matrix (ECM) and compounds like Streptomycin, Heparin, and Penicillin may also play a role in optimizing umbilical vein research.
For endothelial cell studies, the use of EGM-2 (Endothelial Cell Growth Medium-2) and the EA.hy926 cell line, derived from human umbilical vein endothelial cells, can provide valuable insights.
Meanwhile, the RPMI 1640 medium is a commonly used cell culture medium that may be applicable to various umbilical vein-related experiments.
By incorporating these relevant terms and techniques, researchers can streamline their work, ensure more reliable results, and advance our understanding of this vital component of the fetal circulatory system.
Optimizing umbilical vein protocols can lead to breakthroughs in prenatal physiology and fetal development, ultimately improving outcomes for mothers and children.