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Extracellular Matrix

The extracellular matrix (ECM) is a complex, three-dimensional network of macromolecules that provides structural and functional support to cells within tissues.
The ECM is composed of a variety of proteins, including collagen, fibronectin, and laminin, as well as proteoglycans and glycosaminoglycans.
This dynamic microenvironment plays a crucial role in cell adhesion, migration, proliferation, and differentiation, and is essential for tissue homeostasis and repair.
Reserach on the ECM is critical for understanding fundamental biological processes, as well as developing therapies for a wide range of diseases, including cancer, fibrosis, and regenerative medicine.
PubCompare.ai offers a powerful platform to streamline ECM research, leveraging AI-driven comparisons to identify the best protocols and products from literature, pre-prints, and patents, enhacing reproducibility and accuracy.

Most cited protocols related to «Extracellular Matrix»

1
Mapping Protein Conservation Scores Using ConSurf
After the calculation begins, ConSurf produces a status page indicating the computation parameters along with the different stages of the server activity. The main result of a ConSurf calculation is under the link ‘View ConSurf Results with Protein Explorer’, which leads to the graphic visualization of the query protein, color coded by conservation scores, through the Protein Explorer interface (9 (link)). The continuous conservation scores of each of the amino acid positions are available under the link ‘Amino Acid Conservation Scores’, along with the color grades and additional data. The script command for viewing the 3D structure of the query protein, color coded by conservation scores, is available under the link ‘RasMol coloring script source’. This file can be downloaded and used locally with the RasMol program (10 (link)), thus producing the same color-coded scheme generated by the server. A PDB file, in which the conservation scores are specified in the temperature (B) factor field, can be downloaded through the link: ‘The PDB file updated with the conservation scores in the tempFactor field’. Thus, any 3D protein viewer, such as the RasMol program (10 (link)), which is capable of presenting the B factors, is suitable for mapping the conservation scores on the structure.
The ConSurf output also includes links to the PSI-BLAST results, the homologous sequences along with a link to their SWISS-PROT entry page, the MSA and the phylogenetic tree used in the calculation.
As an example, we provide in Figure 2 the main output of a ConSurf run of the Kcsa potassium-channel (11 (link)), a transmembrane protein from Streptomyces Lividans. Kcsa is a homotetramer with a 4-fold symmetry axis about its pore. The ConSurf calculations demonstrate the high level of conservation of the pore region as compared with the rest of the protein. The pore architecture provides the unique stereochemistry which is required for efficient and selective conduction of potassium ions (11 (link)). The biological importance of this stereochemistry is reflected by a strong evolutionary pressure to resist amino acid replacements in the pore. In contrast, the regions that surround the pore and face the extracellular matrix are highly variable.
Landau M., Mayrose I., Rosenberg Y., Glaser F., Martz E., Pupko T, & Ben-Tal N. (2005). ConSurf 2005: the projection of evolutionary conservation scores of residues on protein structures. Nucleic Acids Research, 33(Web Server issue), W299-W302.
Publication 2005
Amino Acids Biological Evolution Biopharmaceuticals Complement Factor B Electric Conductivity Epistropheus Extracellular Matrix Face Homologous Sequences Integral Membrane Proteins Ions Potassium Potassium Channel Pressure Proteins Streptomyces lividans Surgical Replantation
2
Optimized Isolation of Mouse Hepatocytes

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Publication 2020
Berries BLOOD Calcium Cannulation Cell Cycle Checkpoints Cells ChIP-Chip Collagenase Dental Anesthesia Digestion Extracellular Matrix Friend Hepatocyte Liberase Liver Mice, House Percoll Perfusion Veins, Portal Venae Cavae
3
Osteocyte-like phenotype culture
Tissue culture media were purchased from GIBCO BRL, fetal bovine serum (FBS) was from BioWhittaker. Rat tail collagen type 1, 99% pure, was purchased from Becton Dickinson Laboratories. All other reagents were purchased from Sigma Chemical Co. unless otherwise stated. Cells were expanded in permissive conditions (33°C in αMEM with 10% FBS, 100 units/ml penicillin, 50 µg/ml streptomycin, and 50 U/ml IFN-γ) on rat tail type I collagen-coated plates or gels or bovine type I collagen sponges. To induce osteogenesis, cells were plated at 80,000 cells/cm2 in osteogenic conditions (37°C with 50 µg/ml ascorbic acid and 4 mM β-glycerophosphate in the absence of IFN-γ). Collagen-coated surfaces were used because they were found to be effective at maintaining an osteocyte-like phenotype (10 (link)).
MLO-A5 cells, used as controls, are an established model of late osteoblasts with the ability to rapidly synthesize mineralized extracellular matrix (1 (link)). MLO-A5 cells are highly responsive to mechanical loading in 3D culture (15 (link)). MLO-Y4 cells, also used as controls, are an established model of osteocytes.
Woo S.M., Rosser J., Dusevich V., Kalajzic I, & Bonewald L.F. (2011). Cell Line IDG-SW3 Replicates Osteoblast-to-Late-Osteocyte Differentiation in vitro and Accelerates Bone Formation in vivo. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 26(11), 2634-2646.
Publication 2011
Ascorbic Acid beta-glycerol phosphate Bos taurus Cells Collagen Collagen Type I Culture Media Extracellular Matrix Fetal Bovine Serum Gels Interferon Type II Osteoblasts Osteocytes Osteogenesis Penicillins Phenotype Porifera Streptomycin Tail Tissues
4
3D Biofilm Matrix Visualization
The sequential assembly of the matrix was determined by directly incorporating a fluorescent marker during synthesis of the extracellular polysaccharide (EPS)-matrix, allowing examination of the three-dimensional (3D) structure within intact biofilms [63] (link), [64] . Briefly, 1 µM Alexa Fluor 647-labeled dextran conjugate (molecular weight, 10 kDa; absorbance/fluorescence emission maxima of 647/668 nm; Molecular Probes, Invitrogen Corp., Carlsbad, CA) was added to the culture medium from the beginning of and during the development of the biofilms. This technique is based on the observation that (fluorescently-labeled) dextran serves as a primer and acceptor for Gtfs (particularly GtfB), and is incorporated into newly formed glucan by the exoenzyme during synthesis of the EPS-matrix over the course of biofilm development [63] (link); it does not stain the bacterial cells at the concentration used in this study [63] (link). All the bacterial species in the biofilms were labeled by means of SYTO 9 green fluorescent nucleic acid stain (485/498 nm; Molecular Probes) using standard protocols [63] (link), [64] . The imaging was performed using an Olympus FV 1000 two photon laser scanning microscope (Olympus, Tokyo, Japan) equipped with a 10× (0.45 numerical aperture) or 25× LPlan N (1.05 numerical aperture) water immersion objective lens. The excitation wavelength was 810 nm, and the emission wavelength filter for SYTO 9 was a 495/540 OlyMPFC1 filter, while the filter for Alexa Fluor 647 was an HQ655/40M-2P filter [22] (link). Each biofilm was scanned at 5 positions randomly selected at the microscope stage [65] (link), and confocal image series were generated by optical sectioning at each of these positions. Three independent biofilm experiments were performed, and 10 image stacks (512×512 pixel for quantification or 1024×1024 pixel for visualization in tagged image file format) were collected for each experiment.
Xiao J., Klein M.I., Falsetta M.L., Lu B., Delahunty C.M., Yates JR I.I.I., Heydorn A, & Koo H. (2012). The Exopolysaccharide Matrix Modulates the Interaction between 3D Architecture and Virulence of a Mixed-Species Oral Biofilm. PLoS Pathogens, 8(4), e1002623.
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Publication 2012
Alexa Fluor 647 Anabolism Bacteria Biofilms Cell Nucleus Culture Media Dextran Extracellular Matrix Fluorescence Glucans Immersion Laser Scanning Microscopy Lens, Crystalline Light green SF Microscopy Molecular Probes Oligonucleotide Primers Polysaccharides Stains SYTO 9 Vision
5
Generation and Characterization of CFTR-Expressing Bronchial Epithelial Cells
Experiments were performed with CF (CFBE41o−) [51 (link)] and normal (16HBE14o−) [20 (link)] human bronchial epithelial cell lines. The CFBE41o− cell line was originally derived from a bronchial tissue isolate of a CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the pSVori− plasmid that contained a replication-deficient simian virus 40 (SV40) genome [22 (link),25 (link),52 (link),53 (link)]. For the generation of CF cells complemented with wtCFTR and ΔF508CFTR, the parental CFBE41o− cell line was transfected by electroporation (nucleofection; Amaxa Biosystems, Germany) with an Epstein-Barr virus (EBV)-based episomal expression vector, pCEP4β (InVitrogen, Carlsbad, CA) containing either the 6.2 kb full-length wtCFTR cDNA (derived from pBQ6.2, a gift from L-C Tsui and J Rommens) [33 (link)] or the 4.7 kb ΔF508CFTR cDNA, respectively. The 4.7 kb ΔF508CFTR cDNA contained a TTT deletion at the ΔF508 locus rather than the naturally occurring CTT [54 (link),55 (link)] thereby making it possible to differentiate between the expression of endogenous ΔF508CFTR and the plasmid derived ΔF508CFTR. Transfected CFBE41o− cells were grown in the presence of 200–500 µg/ml hygromycin B to select for clones of cells that contained the transfected plasmid. Resistant clones were isolated, expanded and characterized. PCR, reverse transcriptase PCR (RT-PCR), and quantitative PCR and RT-PCR (Q-PCR and QRT-PCR, respectively) were used to confirm the presence and amount of the CFTR transgene and its expression, respectively. Several stable clones were identified and two clones expressing the 6.2 kb wtCFTR cDNA (CFBE41o− c7-6.2wt and CFBE41o− c10-6.2wt) and one expressing the 4.7 kb ΔF508CFTR cDNA (CFBE41o− c4-4.7ΔF) were characterized further. The clones were selected based on their level of transgene derived CFTR mRNA expression. The 16HBE14o− cell line was used as a reference for the expression of endogenous wtCFTR that results in cAMP-dependent Cl transport observed in the normal airway epithelium. Cells were grown in flasks coated with an extracellular matrix cocktail comprised of human fibronectin (BD Biosciences), Vitrogen (Cohesion, Inc.), and bovine serum albumin (Biosource/Biofluids) [12 (link),56 ] in MEM cell culture medium supplemented with 10% fetal calf serum (FCS), 2 mM glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin sulfate under 5% CO2 at 37°C.
Illek B., Maurisse R., Wahler L., Kunzelmann K., Fischer H, & Gruenert D.C. (2008). Cl Transport in Complemented CF Bronchial Epithelial Cells Correlates with CFTR mRNA Expression Levels. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 22(1-4), 57-68.
Publication 2008
Bronchi Cell Culture Techniques Cell Lines Cells Clone Cells Cloning Vectors Culture Media Cystic Fibrosis Transmembrane Conductance Regulator Deletion Mutation DNA, Complementary DNA Replication Electroporation Episomes Epithelium Epstein-Barr Virus Extracellular Matrix Fetal Bovine Serum Fibronectins Genome Glutamine Homo sapiens Homozygote Hygromycin B Mutation Parent Patients Penicillins Plasmids Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Serum Albumin, Bovine Simian virus 40 Streptomycin Sulfate Tissues Transgenes Vitrogen

Most recents protocols related to «Extracellular Matrix»

1
Chitosan Nanofiber Membranes for Tissue Regeneration
Not available on PMC !

Example 12

There has been a growing interest in the fabrication of nanofibers derived from natural polymers due to their ability to mimic the structure and function of extracellular matrix. Electrospinning is a simple technique to obtain nano-micro fibers with customized fiber topology and composition (FIGS. 33A and 33B). The chitosan electrospun nanofibers have recently been extensively studied due to the favorable properties of chitosan such as controllable biodegradation, good biocompatibility and high mechanical strength. Currently, chitosan can be electrospun from a solution of chitosan dissolved in either trifluoroacetic acid (TFA) or acetic acid (HAc). However, processes to remove residual acid and acid salts from the electrospun material generally resulted in a swelling of fibers and deterioration of the nano-fibrous structure. Crosslinking in combination with neutralization methods also had not been effective at preventing loss of nano-fibrous structure.

The current study aimed to improve and maintain nano-fibrous and porous structure of the electrospun membranes by introducing a new post electrospinning chemical treatment. Membrane thickness was tripled in this research in order to increase the general tearing strength. Scanning electron micrograph (SEM) examination (FIG. 33C) and transmission electron micrograph (TEM) examination (FIG. 33D) showed Fiber diameters of the triethanolamine/N-tert-butoxycarbonyl (TEA/t-BoC) treated membranes ranged from 40 nm to 130 nm while fiber diameters were not able to be determined for the Na2CO3 group. Membranes treated by TEA/tboc (FIG. 34A) exhibited more nano-scale fibrous structure than membranes treated by saturated Na2CO3 (FIGS. 35B-35D, as seen demonstrated in scanning electron micrographs. After immersion in PBS for 24 hours, membranes treated by TEA/tboc exhibited less than 30% swelling (FIG. 34B) and retained their nanofibrous structure, compared with membranes treated by Na2CO3 (FIGS. 35B-35D) or compared with the non-treated chitosan membrane (FIG. 35A). After soaking the TEA/tBoc treated membranes in water overnight, membranes still kept the porous structure. In both, the before and after water status, fibers kept diameters in the nanometer range (FIG. 35C). TEA/tBoC modified nanofiber membranes also well preserved their fibrous structure over 4 weeks in physiological solution compared with Na2CO3 treated membranes (FIG. 35D).

Chitosan membranes treated by TEA/tboc showed better nano-fiber morphology characteristics than membranes neutralized by saturated Na2CO3 solution before and after being soaked in PBS. Retention of the nanofibrous structure for guided tissue regeneration applications may be of benefit for enabling nutrient exchange between soft gingival tissue and bone compartments and for mimicking the natural nanofibrillar components of the extracellular matrix during regeneration.

US11878088B2. Chitosan nanofiber compositions, compositions comprising modified chitosan, and methods of use (2024-01-23). The University of Memphis Research Foundation [US]. Inventors: Joel D. Bumgardner [US], Hengjie Su [US], Tomoko Fujiwara [US], Daniel G. Abebe [US], Kwei-Yu Liu [US].
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Patent 2024
Acetic Acid Acids Bones Chitosan Electrons Environmental Biodegradation Extracellular Matrix Fibrosis Gingiva Guided Tissue Regeneration Hydrochloric acid Nutrients physiology Polymers Regeneration Retention (Psychology) Submersion TERT protein, human Tissue, Membrane Tissues Transmission, Communicable Disease triethanolamine Trifluoroacetic Acid Vision
2
BMP-7 Mimicking Peptide Enhances Cartilage ECM
Not available on PMC !

Example 7

Cartilage explants obtained from 2 patients were cultured for 14 days in the presence of BMP-7 (1 nM) or BMP-7 mimicking peptide GYAAYYSEGESAFPLNSYMN (SEQ ID NO: 8) at 10 nM. Glycosaminoglycans (GAGs), an important component of the extracellular matrix (ECM), were stained with Safranin-O (in red) and other tissues are counterstained with Fast green (in green/blue).

Both patients showed an increased Safranin-O intensity in BMP7 and peptide GYAAYYSEGESAFPLNSYMN (SEQ ID NO: 8) treated explants compared to control.

These results are in line with the effects described above and show the BMP-7 mimicking bioactivity of the peptides according to the invention.

US11872264B2. Method for the treatment or prevention of osteoarthritis (2024-01-16). Chondropeptix B.V. [NL]. Inventors: Tim Johannes Maria Welting [NL], Marjolein Maria Johanna Caron [NL].
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Patent 2024
BMP7 protein, human Cartilage Extracellular Matrix Fast Green Glycosaminoglycans Patients Peptides safranine T Tissues
3
Migratory and Invasive Abilities of Cancer Cells
Not available on PMC !

Example 3

Cell migration is a highly-integrated and multi-step process that plays an important role in the progression of late-stage cancer. Cell invasion is involved in extracellular matrix degradation and proteolysis. In the study, wound healing assay and transwell invasion assay were used to examine migratory and invasive abilities of PDV cells, respectively, with or without PLX4032 stimulation. In invasion assay, PLX4032 promoted the invasive ability of PDV cells (FIG. 3). Further, in the presence or absence of PLX4032, KWM-EO, LM-EO and L+C treatment for 24 h reduced invaded cells on concentration-dependence.

In wound healing assay, 50 μg/mL KWM-EO, 50 μg/mL LM-EO and 40 μg/mL L+C reduced PDV cell migratory ability at 24 h treatment, and LM-EO had a better effect than the others (FIG. 4). On the other hand, 2 μM PLX4032 treatment strongly promoted cell migration of PDV cells within 24 h treatment, KWM-EO, LM-EO and L+C combination, similarly both EOs and compounds only, significantly suppressed PLX4032-stimulated migratory ability of PDV cells.

US11872260B2. Mint essential oils inhibit HRASQ61L mutant keratinocyte activity and prevent skin carcinogenesis (2024-01-16). ACADEMIA SINICA [TW]. Inventors: Lie-Fen Shyur [TW], Chih-Ting Chang [TW], Yuhsin Chen [TW].
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Patent 2024
Biological Assay Cells Disease Progression Extracellular Matrix Mentha Migration, Cell Oils, Volatile PLX4032 Proteolysis Staging, Cancer
4
Establishment and Characterization of PDTO
Tumor pieces will be dissociated enzymatically and mechanically to obtain isolated cells or small cell clusters (Fig. 2). Cells will be embedded in an extracellular matrix (growth factor-reduced Matrigel or BME II) and cultured in a medium supplemented with growth factors and signal pathway inhibitors [Advanced DMEM (Gibco) supplemented with 100 UI/mL of penicillin and streptomycin (Gibco), 1% GlutaMAX (Gibco), 1X B27 (Gibco), 1.25 mM NAC (Sigma-Aldrich), 50 ng/mL EGF (PeproTech), 10 ng/mL FGF-10 (PeproTech), 5 ng/mL FGF-b (PeproTech), 500 nM A-83-01 (PeproTech), 10 μM Y27632 (Interchim), 10 mM Nicotinamide (Sigma-Aldrich), 1 μM PGE2 (PeproTech), 1 μM Forskolin (Peprotech), 0.3 μM CHIR99021 (Biogems), 100 μg/mL Primocin (InvivoGen), 50% Wnt3a, RSPO3, Noggin-conditioned media (L-WRN, ATCC), and 10% RSPO1-conditioned media (Cultrex HA-R-Spondin-1-Fc 293 T, Amsbio)]. Culture medium will be changed twice a week. Once formed, PDTO will be dissociated and reseeded to amplify them for experimental purposes. Cryovials will be prepared at regular intervals by dissociating and resuspending PDTO in Recovery Cell Culture Freezing Medium (Gibco) prior to be biobanked in liquid nitrogen. It should be noted that PDTO line will be considered as established when it will be maintained for more than 3 passages. For each PDTO line, samples will be kept frozen for DNA/RNA/protein analysis and others will be embedded in paraffin for histopathological analysis. This will allow comparisons between the characteristics of the PDTO and the tumor from which they are derived in order to validate their correspondence.

Establishment and characterization of PDTO derived from HNSCC and evaluation of response to treatments to assess its predictive value

Perréard M., Florent R., Divoux J., Grellard J.M., Lequesne J., Briand M., Clarisse B., Rousseau N., Lebreton E., Dubois B., Harter V., Lasne-Cardon A., Drouet J., Johnson A., Le Page A.L., Bazille C., Jeanne C., Figeac M., Goardon N., Vaur D., Micault E., Humbert M., Thariat J., Babin E., Poulain L., Weiswald L.B, & Bastit V. (2023). ORGAVADS: establishment of tumor organoids from head and neck squamous cell carcinoma to assess their response to innovative therapies. BMC Cancer, 23, 223.
Full text: Click here
Publication 2023
Cell Culture Techniques Cells Chir 99021 Colforsin Culture Media Culture Media, Conditioned Dinoprostone Extracellular Matrix Freezing Growth Factor HSP40 Heat-Shock Proteins inhibitors matrigel Neoplasms Niacinamide Nitrogen noggin protein Paraffin Embedding Penicillins Signal Pathways Squamous Cell Carcinoma of the Head and Neck Streptomycin Y 27632
5
Evaluating PDTO Responses to Diverse Treatments
The evaluation of the response to treatments will be performed when PDTO have reached a diameter of 150 μm in « PDTO treatment medium », corresponding to the PDTO culture medium lacking N-Acetylcysteine, Y-27632 and primocin.
PDTO will be collected, resuspended in 2% extracellular matrix/PDTO culture medium and then platted in white and clear bottom 96-well plates previously coated with a 1:1 volume mix of PDTO treatment medium with extracellular matrix. In the case of evaluation of the response to radiotherapy, PDTO will be before irradiated using the CellRad System (FAXITRON Bioptics). In the case of evaluation of the response to chemotherapy or PARP inhibitors, drugs are prepared in 2% extracellular matrix/PDTO culture medium and added 1 hour after PDTO have been plated.
In the case of evaluation of the response to immunotherapies, PDTO will be co-cultured with PDTO specific T cells previously generated (see co-culture of PDTO with immune cells) at a 5:1 ratio. Treatments (such as Nivolumab or Pembrolizumab) will be added directly in the co-culture. A condition containing an MHC-I blocking antibody will be added to control for antigen specific killing.
PDTO morphology will be monitored by taking images during the required time using Incucyte S3 (Sartorius). At the endpoint, PDTO response will be assessed using CellTiter-Glo 3D cell viability assay (Promega) according to the manufacturer’s instruction and luminescence will be measured using GloMax Discover GM3000 (Promega) with the associated software. Results will be normalized to the control condition. IC50 will be calculated with GraphPad software. The ability of T cells to recognize and induce lysis of PDTO will be monitored via analysis of caspase 3 cleavage within PDTO and visualization of LAMP-1 on the membrane of CD8+ T cells.
The treatment response of the PDTO will be finally compared to the clinical response (PFS/DFS/OS) of the patient from whom they are derived in order to validate the predictive value of this model for HNSCC.
Perréard M., Florent R., Divoux J., Grellard J.M., Lequesne J., Briand M., Clarisse B., Rousseau N., Lebreton E., Dubois B., Harter V., Lasne-Cardon A., Drouet J., Johnson A., Le Page A.L., Bazille C., Jeanne C., Figeac M., Goardon N., Vaur D., Micault E., Humbert M., Thariat J., Babin E., Poulain L., Weiswald L.B, & Bastit V. (2023). ORGAVADS: establishment of tumor organoids from head and neck squamous cell carcinoma to assess their response to innovative therapies. BMC Cancer, 23, 223.
Full text: Click here
Publication 2023
Acetylcysteine Antibodies, Blocking Antigens Biological Assay Caspase 3 CD8-Positive T-Lymphocytes Cells Cell Survival Coculture Techniques Cultured Cells Culture Media Cytokinesis Extracellular Matrix Immunotherapy Luminescence lysosomal-associated membrane protein 1, human Nivolumab Patients pembrolizumab Pharmaceutical Preparations Pharmacotherapy Poly(ADP-ribose) Polymerase Inhibitors Promega Squamous Cell Carcinoma of the Head and Neck T-Lymphocyte Tissue, Membrane Y 27632

Top products related to «Extracellular Matrix»

1
Matrigel by BD
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Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
2
Alizarin red s by Merck Group
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Alizarin Red S is a chemical compound used as a dye and a stain in laboratory procedures. It is a red-orange powder that is soluble in water and alcohol. Alizarin Red S is commonly used to stain calcium deposits in histological samples, such as bone and cartilage.
3
Fetal bovine serum (fbs) by Thermo Fisher Scientific
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
4
Matrigel by Corning
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Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
5
Dexamethasone (dex) by Merck Group
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Dexamethasone is a synthetic glucocorticoid medication used in a variety of medical applications. It is primarily used as an anti-inflammatory and immunosuppressant agent.
6
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
7
Rt2 first strand kit by Qiagen
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The RT2 First Strand Kit is a laboratory reagent used for the reverse transcription of RNA to cDNA. It provides the necessary components for the conversion of RNA to cDNA, which is a crucial step in various molecular biology applications, such as gene expression analysis and real-time PCR.
8
Ascorbic acid by Merck Group
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Ascorbic acid is a chemical compound commonly known as Vitamin C. It is a water-soluble vitamin that plays a role in various physiological processes. As a laboratory product, ascorbic acid is used as a reducing agent, antioxidant, and pH regulator in various applications.
9
Fibronectin by Merck Group
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Fibronectin is an extracellular matrix glycoprotein that plays a role in cell adhesion, growth, migration, and differentiation. It is a key component of the cellular microenvironment and is involved in various biological processes.
10
Transwell chamber by Corning
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Transwell chambers are a type of lab equipment used for cell culture and biological assays. They consist of a permeable membrane insert placed inside a well, allowing for the study of cell-cell interactions and the movement of molecules across a barrier. The core function of Transwell chambers is to provide a controlled environment for culturing cells and monitoring their behavior and permeability.

More about "Extracellular Matrix"

Extracellular Matrix (ECM) is a complex, three-dimensional network of macromolecules that provides structural and functional support to cells within tissues.
This dynamic microenvironment is composed of a variety of proteins, including collagen, fibronectin, laminin, and proteoglycans, as well as glycosaminoglycans.
The ECM plays a crucial role in cell adhesion, migration, proliferation, and differentiation, and is essential for tissue homeostasis and repair.
Research on the ECM is critical for understanding fundamental biological processes, as well as developing therapies for a wide range of diseases, including cancer, fibrosis, and regenerative medicine.
Matrigel, a widely used basement membrane extract, is a popular ECM-mimicking material that supports cell growth and differentiation.
Alizarin Red S, a staining agent, is often used to detect mineralized extracellular matrix, such as in bone and cartilage studies.
Serum components like Fetal Bovine Serum (FBS) and supplements like Dexamethasone, Penicillin/Streptomycin, and Ascorbic acid can influence the composition and behavior of the ECM.
The RT2 First Strand Kit is a tool used for gene expression analysis related to the ECM.
Fibronectin, a key ECM protein, supports cell adhesion and migration, and is often used in cell culture systems.
Transwell chambers are commonly employed to study cell migration and invasion through the ECM.
PubCompare.ai offers a powerful platform to streamline ECM research, leveraging AI-driven comparisons to identify the best protocols and products from literature, pre-prints, and patents, enhaning reproducibility and accuracy.
Optimize your extracellular matrix research with PubCompare.ai and make data-driven decisions with our platform.