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> Anatomy > Tissue > Mesothelium

Mesothelium

Mesothelium is a thin, protective layer of cells that lines the internal body cavities, including the chest (pleura), abdomen (peritoneum), and heart (pericardium).
This specialized epithelial tissue plays a crucial role in reducing friction and facilitating the smooth movement of organs within these cavities.
Mesothelial cells also secrete lubricating fluids that further enhance the efficiency of these internal processes.
Understanding the structure, function, and pathologies of the mesothelium is essential for research into a variety of conditions, such as mesothelioma, a rare and aggresive form of cancer that originates in the mesothelial cells.
PubCompare.ai can help optimize your mesothelium research by providing access to the best available protocols, preprints, and patents, enabling more reproducible and accurate investigations.

Most cited protocols related to «Mesothelium»

1
Single-Cell RNA-seq Data Integration

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Publication 2019
Cells Dendrites Mesothelium
2
Isolation of Mesothelial Cells from Murine Peritoneum
Mesothelial cells were isolated from 4 to 6 months old WT and Nf2+/− mice, all on a C57Bl/6J background using a previously described protocol (Bot et al.2003 (link)). Briefly, mice were killed and the peritoneal cavities were exposed by incision and removal of the fur. The peritoneal cavities were washed with injecting approximately 50 ml of phosphate-buffered saline (PBS) (Sigma, St. Louis) via a syringe equipped with a 25G × 5/8″ needle (BD microlance 3, Becton Dickinson AG, Allschwil, Switzerland) using a peristaltic pump and a second needle (23G, 0.6 × 25 mm) to allow exit of the PBS solution. Perfusion was maintained until the exiting PBS solution was clear, i.e., devoid of mobile and poorly attached cells. Residual PBS was aspired with a syringe, and the peritoneal cavity was filled with 5 ml of 0.25% Trypsin/EDTA solution (Gibco, Basel, Switzerland). The body temperature of mouse corpses was maintained at around 37°C for 5 min via an infrared heating lamp. The suspension containing the detached cells was collected with a syringe; cells were centrifuged for 10 min at 300×g. Cells mostly comprising primary mesothelial cells were grown in modified Connell’s medium composed of Dulbecco’s modified Eagle’s medium (DMEM)/F12 + GlutaMax (Gibco), 15% fetal calf serum (FCS), 0.4 μg/ml hydrocortisone, 10 ng/ml epidermal growth factor, 1% ITS (insulin, transferrin, selenium), 1 mM sodium pyruvate, 0.1 mM beta-mercaptoethanol, 1% non-essential amino acids, 1% penicillin–streptomycin, and 2% Mycokill (PAA) (Connell and Rheinwald 1983 (link)). The Nf2 alleles (WT or mutated) were genotyped using the common forward primer (NF2_FW 5′-GGGGCTTCGGGAAACCTG G-3′), and either NF2_RV WT (5′-GTCTGGGAAGTCTGTGGAGG-3) or NF2_RV mutant (5′-CTATCAGGACATAGCGTTGG-3′) primers. The cell line RN5 was isolated from an Nf2+/− mouse that was repeatedly injected with crocidolite starting at 8 wk of age (7 × 400 μg). Briefly, a clearly discernible tumor localized on the liver was dissected from the mouse 21 wk after the first injection. The tissue was incubated in a 0.25% Trypsin/EDTA solution for 10 min; tumor cells were dissociated by mild trituration and cultured in DMEM, 10% fetal bovine serum (FBS, Gibco, Basel, Switzerland), and 1% PS (100 U/mL penicillin and 100 μg/mL streptomycin).
Blum W., Pecze L., Felley-Bosco E., Worthmüller-Rodriguez J., Wu L., Vrugt B., de Perrot M, & Schwaller B. (2015). Establishment of immortalized murine mesothelial cells and a novel mesothelioma cell line. In Vitro Cellular & Developmental Biology. Animal, 51(7), 714-721.
Publication 2015
2-Mercaptoethanol Alleles Amino Acids, Essential Asbestos, Crocidolite Body Temperature Cadaver Cell Lines Cells Culture Media Eagle Edetic Acid Epidermal growth factor Hydrocortisone Insulin Liver Mesothelium Mus Needles Neoplasms Oligonucleotide Primers Penicillins Perfusion Peristalsis Peritoneal Cavity Phosphates Pyruvate Saline Solution Selenium Sodium Streptomycin Syringes Tissues Transferrin Trypsin
3
Integrative Single-Cell Atlas of Adipose Tissue
Integration, clustering and subclustering analysis were performed using Seurat 3.9.965 . The gene counts were normalized using SCTransform66 , and regressed on mitochondrial read percentage, ribosomal read percentage, and cell cycle score as determined by Seurat. In order to avoid smoothing over depot differences, for integration human and mouse data were grouped by ‘individual’, i.e., if both subcutaneous and visceral adipose tissue for an individual human or mouse were available, they were pooled together during this step. Individuals were integrated with reciprocal PCA, using individuals that had both subcutaneous and visceral samples as references. As a result, the human and mouse references were comprised exclusively from the sNuc seq cohort. To integrate, references were integrated together, then the remaining samples—sNuc seq individuals with only subcutaneous data as well as all Drop-seq samples—were mapped to the reference. For clustering, 5000 variable genes were used, and ribosomal and mitochondrial genes were removed from the variable gene set before running PCA and calculating clusters using a Louvain algorithm, 40 PCs, and a resolution of 0.5. Clusters were identified as adipocytes, preadipocytes, mesothelial cells, vascular cells, or immune cells using marker genes, subset into individual objects, and re-integrated using the above method. Samples with fewer than 50 cells in the subset were removed before re-integration. This led to samples having artificially fewer cells in some instances—for example some Drop-seq samples had cells that clustered with adipocytes, but these cells were removed in subclustering because the small numbers of cells introduced too much variability into the integration. Subclustering was performed using a range of variable genes (1000-2000), PCs (10-40) and resolutions (0.2-0.6). Markers were calculated using a non-parametric Wilcoxon rank sum test with p values adjusted using Bonferroni correction (Supplementary Tables 1, 2), and clusters were evaluated based on the distinctness of called markers to determine the final subclustering conditions. In the subclustered objects, we removed clusters that appeared to represent doublets based on the score assigned by scrublet61 , or that appeared to be driven by high ambient RNA content as determined by percentage of mitochondrial genes and spliced/unspliced RNA ratio. The remaining clusters were annotated based on marker gene expression. In some cases, smaller subclusters (T and NK cells, B cells, monocytes/neutrophils) were further subset and PCA and clustering analysis but not integration was re-run in order to assign clusters. After subcluster annotation, identities were mapped back onto the original object and cells that were removed from the subclustered objects were similarly removed from the all-cell object.
Emont M.P., Jacobs C., Essene A.L., Pant D., Tenen D., Colleluori G., Di Vincenzo A., Jørgensen A.M., Dashti H., Stefek A., McGonagle E., Strobel S., Laber S., Agrawal S., Westcott G.P., Kar A., Veregge M.L., Gulko A., Srinivasan H., Kramer Z., De Filippis E., Merkel E., Ducie J., Boyd C.G., Gourash W., Courcoulas A., Lin S.J., Lee B.T., Morris D., Tobias A., Khera A.V., Claussnitzer M., Pers T.H., Giordano A., Ashenberg O., Regev A., Tsai L.T, & Rosen E.D. (2022). A single cell atlas of human and mouse white adipose tissue. Nature, 603(7903), 926-933.
Publication 2022
Adipocytes B-Lymphocytes Blood Cells Cell Cycle Cells Gene Expression Genes Genes, Mitochondrial Genes, vif Homo sapiens Mesothelium Mitochondrial Inheritance Monocytes Mus Natural Killer Cells Neutrophil Ribosomes Visceral Fat
4
Evaluation of Intraperitoneal Injection Accuracy
Following CHB, each animal was carefully removed from the chamber and positioned in dorsal recumbency for necropsy examination. The skin was incised along the midline and the injection site was identified in the abdominal wall musculature. The abdominal wall was incised and the intestines were reflected out of the abdominal cavity. Distribution of blue injectate and any misinjection into hollow viscera were noted. The liver was reflected cranially and any presence of dye within the biliary vessels caused by uptake of injectate from the peritoneal cavity and subsequent biliary excretion was noted. The GIT from the cardia to the descending colon was removed and any intestinal segments with dye-stained serosa were opened to confirm or rule out intraluminal misinjection. Misinjection was defined as the presence of blue injectate within hollow viscera or subcutaneous tissues, or staining the fur. For each rat, the serosal surfaces of the abdominal wall injection site, the caudate liver lobe, and transverse sections of at least three intestinal sections were examined histologically after formalin fixation for evidence of acute inflammation or swelling of mesothelial cells. Evaluation was performed by a single board-certified veterinary pathologist (CK), who was blinded to treatment group assignments.
Zatroch K.K., Knight C.G., Reimer J.N, & Pang D.S. (2017). Refinement of intraperitoneal injection of sodium pentobarbital for euthanasia in laboratory rats (Rattus norvegicus). BMC Veterinary Research, 13, 60.
Publication 2016
Abdominal Cavity Animals Autopsy Biliary Elimination Blood Vessel Cardia Cells Colon, Descending Formalin Inflammation Intestines Liver Mesothelium Pathologists Peritoneal Cavity Serous Membrane Skin Subcutaneous Tissue Viscera Wall, Abdominal
5
Porcine Model of Ventral Hernia Repair

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Publication 2011
Abdominal Cavity Abdominal Muscles Alloderm Anesthesia Animals Animals, Laboratory Antibiotic Prophylaxis Aponeurosis Areola Biopharmaceuticals Bladder Detrusor Muscle Cattle Cells Cephalexin Creativity Cyanoacrylates Dermis Eosin Euthanasia Fascia Feces Fibrosis Grafts Grasp Hernia Herniorrhaphy Homo sapiens Inflammation Innovativeness Ketamine Light Microscopy Mesothelium Microtomy Operative Surgical Procedures Paraffin Embedding Pathologic Neovascularization Pathologists Pentobarbital Pericardium Peritoneum Permacol Pharmaceutical Preparations Pigs Polydioxanone Postoperative Care Potassium Chloride Prolene Sterility, Reproductive Subcutaneous Fat Sutures Swine, Miniature Telazol Tissues Transversus Abdominis Wall, Abdominal Woman Xylazine

Most recents protocols related to «Mesothelium»

1
Single-cell analysis of ovarian cancer
The data set consists of expression data for single cells obtained from 9 patients (4 with matching primary and metastatic samples) in three locations and thus cellular categories: a primary tumor site (ovary or fallopian tube) with 1,649 cells, metastatic site (omentum) with 1,062 cells, and normal site (ovary) with 355 cells. The normal cells are comprised of fibroblasts, stromal cells, and mesothelial cells. When we perform PCA on all the cells, the normal cells do not cluster by cell type and the primary cells do not cluster by location.
Lambros M., Sella Y, & Bergman A. (2023). Phenotypic pliancy and the breakdown of epigenetic polycomb mechanisms. PLOS Computational Biology, 19(2), e1010889.
Publication 2023
Cells Fallopian Tubes Fibroblasts Mesothelium Neoplasms Omentum Ovary Patients Stromal Cells
2
Ovarian Cancer Cell Line Cultivation
All experiments used ovarian cancer tumor cell lines OV-90, OVCAR-3, and OVCAR-8 (ATCC). Cell lines were authenticated by human short tandem repeat (STR) analysis at the TRIP Lab at the University of Wisconsin–Madison. Cells were cultured in 1:1 Medium 199 (with Earle's salts and L-glutamine, Sigma-Aldrich) and MCDB 105 medium (Sigma-Aldrich). Experiments were conducted in serum-free medium, and maintenance culture included 15% heat-inactivated fetal bovine serum (FBS). LP-9 mesothelial cells (Coriell) were cultured in 1:1 Medium 199 and Ham's F-12 supplemented with 200 ng/mL hydrocortisone, 5 ng/mL epidermal growth factor, 0.75 mM L-glutamine, and 10% FBS. All media included 1% penicillin–streptomycin.
Micek H.M., Rosenstock L., Ma Y., Hielsberg C., Montemorano L., Gari M.K., Ponik S.M, & Kreeger P.K. (2023). Model of collective detachment in high-grade serous ovarian cancer demonstrates that tumor spheroids produce ECM to support metastatic processes. APL Bioengineering, 7(1), 016111.
Publication 2023
Cell Line, Tumor Cell Lines Cells Epidermal growth factor Fetal Bovine Serum Glutamine Homo sapiens Hydrocortisone Mesothelium Ovarian Neoplasm Penicillins Salts Serum Short Tandem Repeat Streptomycin
3
Culturing Peritoneal Tissue Samples
Fresh peritoneal tissue was obtained directly from the operating room, with informed consent from the patients, and transferred immediately to the laboratory in E199 medium (Biochrom AG, Berlin, Germany). The tissue samples were then incubated for 15 min in phosphate-buffered saline (PBS) containing penicillin/streptomycin and amphotericin B (Sigma-Aldrich Chemie, St. Louis, MO, USA). Extraperitoneal fat was removed, and 7 × 7 mm tissue pieces were inserted between two stainless steel rings, which are available in various sizes, and thus enable large variations in the setting of these experiments. Moreover, stainless steel rings are more stable than plastic rings. In this setup, the ring system allows for tissue orientation with the mesothelial cells pointing upwards, thereby better reflecting the peritoneal cavity in the in vivo setting. The peritoneum was cultured in E199 medium containing penicillin/streptomycin, L-glutamine (Biochrom AG), FCS (Thermo Fisher Scientific), hydrocortisone (Sigma-Aldrich Chemie), fibroblast growth factor (PeproTech GmbH, Hamburg, Germany), and heparin (Biochrom AG), as described previously [12 (link)].
Koch J., Beil J., Berchtold S., Mönch D., Maaß A., Smirnow I., Schenk A., Carter M.E., Kloker L.D., Leibold T., Renner P., Dahlke M.H, & Lauer U.M. (2023). Establishing a New Platform to Investigate the Efficacy of Oncolytic Virotherapy in a Human Ex Vivo Peritoneal Carcinomatosis Model. Viruses, 15(2), 363.
Publication 2023
Amphotericin B Cells Culture Media Fibroblast Growth Factor Glutamine Heparin Hydrocortisone Mesothelium Patients Penicillins Peritoneal Cavity Peritoneum Phosphates Retroperitoneal Fat Saline Solution Stainless Steel Streptomycin Tissues
4
Isolation of Membrane-Enriched Fraction from MeT5A Cells
A total of 100 × 106 Met5a cells (a human mesothelial cell line, ATCC CRL-9444) were grown in M199 medium supplemented with 10% heat-inactivated fetal bovine serum (FBS), L-glutamine 2 mM, 100 U mL−1 penicillin and 100 U mL−1 streptomycin, 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin at 37 °C in a 5% CO2 atmosphere in 250 ml flasks. Confluent MeT5A cultures were detached with trypsin, washed, counted in a Beckman Coulter Counter Z1, and stored at −20 °C until re-suspended in 25 mL of Tris-HCl buffer 20 mM pH 7.0 containing proteinase inhibitors (cOmplete Protease Inhibitor Cocktail, one tablet dissolved in 50 ml extract). The cells were disrupted in an N2-cavitator at 350 psi for 20 min at 4 °C and the lysate centrifuged at 1000× g for 20 min at 4 °C. The post-nuclear (PNS) fraction was carefully withdrawn and centrifuged in a himac CS150NX (Hitachi) micro ultracentrifuge for 60 min at 4 °C at 100,000× g. The pellet (p100 MET), a membrane rich fraction, and the cytosol (s100) were carefully separated. P100 MET was extracted in 10 mL of 20 mM Tris-HCl pH 7.0 containing 1.5% β−octyl-glucopiranoside (OG) and 1 M NaCl, overnight at 4 °C. The extract was centrifuged at 1000× g to avoid precipitates and extensively dialyzed against 20 mM Tris-HCl pH 7.0. The dialysate was re-centrifuged at 20,000× g in the Hitachi centrifuge to eliminate few small precipitates of unknown origin and filtered through a 0.2 μm pore size membrane (Minisart filter, Millipore, Sigma-Aldrich, Merck, Darmstadt, Germany).
Bernareggi A., Zangari M., Constanti A., Zacchi P., Borelli V., Mangogna A., Lorenzon P, & Zabucchi G. (2023). Asbestos Fibers Enhance the TMEM16A Channel Activity in Xenopus Oocytes. Membranes, 13(2), 180.
Publication 2023
Atmosphere Cell Lines Cells Cytosol Dialysis Solutions Epidermal growth factor Fetal Bovine Serum Glutamine Homo sapiens Hydrocortisone Insulin Mesothelium Penicillins Protease Inhibitors Reproduction S100 Proteins Sodium Chloride Strains Streptomycin Tablet Tissue, Membrane TPX2 protein, human Tromethamine Trypsin
5
Comparative Culturing of Mesothelioma Cells
A normal human mesothelial cell line (MeT-5A) and a malignant human mesothelioma cell line (JU77) were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). Both cell lines have been cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium supplemented with 10% fetal bovine serum, 1% L-glutamine (Lonza; Walkersville, MD, USA), 1% non-essential amino acids solution (Gibco by Thermo Fisher, Waltham, MA, USA), and 1% penicillin/streptomycin (Lonza; Walkersville, MD, USA). The culture conditions were 37 °C in a humidified atmosphere with 5% CO2. The MeT-5A and JU77 cell lines were split 1:3 and 1:6, respectively, twice a week.
Cells in confluent condition were separated from the culture flask (SPL Life Sciences; Korea) using 0.25% trypsin in 2.21 mM EDTA solution (Corning; Manassas, VA, USA) and counted using Bürker chamber by Trypan Blue Stain 0.4% (Gibco by Life Technologies; New York, NY, USA). The cells used for the experiments were between II/III passages.
Filetti V., Lombardo C., Loreto C., Dounias G., Bracci M., Matera S., Rapisarda L., Rapisarda V., Ledda C, & Vitale E. (2023). Small RNA-Seq Transcriptome Profiling of Mesothelial and Mesothelioma Cell Lines Revealed microRNA Dysregulation after Exposure to Asbestos-like Fibers. Biomedicines, 11(2), 538.
Publication 2023
Amino Acids, Essential Atmosphere Cell Lines Cells Culture Media Edetic Acid Fetal Bovine Serum Glutamine Homo sapiens Malignant Mesothelioma Mesothelium Penicillins PRSS2 protein, human Stains Streptomycin Trypan Blue

Top products related to «Mesothelium»

1
Met 5a by American Type Culture Collection
Sourced in United States, Italy
The MeT-5A is a laboratory equipment product designed for cell culture applications. It serves as an incubator that maintains optimal environmental conditions, such as temperature and humidity, to support the growth and maintenance of cell lines.
2
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
3
Msto 211h by American Type Culture Collection
Sourced in United States, Japan, United Kingdom, Switzerland
MSTO-211H is a human malignant pleural mesothelioma cell line. It is a commonly used in vitro model for the study of mesothelioma.
4
Penicillin by Thermo Fisher Scientific
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
5
Streptomycin by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, France, Canada, Australia, Japan, Switzerland, Italy, Belgium, Israel, Austria, Spain, Netherlands, Poland, Brazil, Denmark, Argentina, Sweden, New Zealand, Ireland, India, Gabon, Macao, Portugal, Czechia, Singapore, Norway, Thailand, Uruguay, Moldova, Republic of, Finland, Panama
Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
6
H2052 by American Type Culture Collection
Sourced in United States
H2052 is a cell culture incubator that provides a controlled environment for the growth and maintenance of cells. It maintains precise temperature, humidity, and gas concentration settings to support optimal cell culture conditions.
7
Hydrocortisone by Merck Group
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Hydrocortisone is a laboratory-grade reagent used in various research and analytical applications. It is a synthetic corticosteroid compound with anti-inflammatory and immunosuppressant properties. Hydrocortisone is commonly utilized as a standard or reference material in analytical procedures, such as assays and chromatographic techniques, to quantify and identify related compounds.
8
Rpmi 1640 by Thermo Fisher Scientific
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
9
H2452 by American Type Culture Collection
Sourced in United States
The H2452 is a laboratory centrifuge designed for general-purpose applications. It is capable of separating samples at high speeds, allowing for the efficient isolation of cellular components, proteins, or other biomolecules. The centrifuge features a robust construction and user-friendly controls for reliable and consistent performance.
10
Nci h2452 by American Type Culture Collection
Sourced in United States, Germany, Japan
The NCI-H2452 cell line is a human mesothelioma cell line derived from the pleural effusion of a 52-year-old male patient. The cell line is routinely used in cancer research and drug development studies.

More about "Mesothelium"

The mesothelium is a thin, specialized layer of epithelial cells that lines the body's internal cavities, including the chest (pleura), abdomen (peritoneum), and heart (pericardium).
This protective tissue plays a crucial role in reducing friction and facilitating the smooth movement of organs within these cavities.
Mesothelial cells secrete lubricating fluids that further enhance the efficiency of these internal processes.
Understanding the structure, function, and pathologies of the mesothelium is essential for research into a variety of conditions, such as mesothelioma, a rare and aggressive form of cancer that originates in the mesothelial cells.
Researchers often utilize cell lines like MeT-5A, MSTO-211H, H2052, H2452, and NCI-H2452 to study mesothelial biology and mesothelioma.
Culturing mesothelial cells typically involves media like RPMI 1640 supplemented with fetal bovine serum (FBS), penicillin, streptomycin, and hydrocortisone.
These components provide the necessary nutrients, antibiotics, and growth factors to maintain and propagate mesothelial cell cultures in the laboratory.
By leveraging the insights gained from the mesothelium's characteristics and the available cell lines, researchers can optimize their investigations and gain a deeper understanding of this important tissue.
This knowledge is crucial for advancing research into mesothelioma and other conditions related to the mesothelium.