Animals: C57BL/6 (Jackson Laboratory), C57BL/6 aged mice (National Institutes of Aging), Dcx-Luc20 (link), and C57BL/6J-Act-GFP (Jackson Laboratory). All animal use was in accordance with institutional guidelines approved by the VA Palo Alto Committee on Animal Research. Parabiosis surgery followed previously described procedures19 (link) with the addition that peritonea between animals were surgically connected. Immunohistochemistry followed standard published techniques24 (link). Extracellular electrophysiology was performed as previously described25 (link). Spatial learning and memory was assayed with the RAWM paradigm as previously published26 (link). Contextual fear conditioning was assayed as previously published27 (link). Relative plasma concentrations of cytokines and signaling molecules in mice and humans were measured using antibody-based multiplex immunoassays at Rules Based Medicine, Inc. Statistical analysis was performed with Prism 5.0 software (GraphPad Software). Plasma protein correlations were analyzed with the Significance Analysis of Microarray software (SAM 3.00 algorithm; http://www.stat.stanford.edu/~tibs/SAM/index.htm ). Experiments were carried out by investigators blinded to the treatment of animals.
Peritoneum
The peritoneum is a thin, smooth, serous membrane that lines the abdominal cavity and covers most of the abdominal organs.
It plays a crucial role in the body's immune response and fluid balance.
The peritoneum is composed of a single layer of squamous epithelial cells, known as mesothelial cells, which rest on a basement membrane.
This membrane provides a slippery surface that allows the abdominal organs to move freely during normal bodily functions.
Peritoneal research is essential for understanding various abdominal and pelvic conditions, such as peritonitis, ascites, and abdominal adhesions.
Optimize your peritoneum studies with the innovative PubCompare.ai tool, which can help you locate the best protocols from literature, pre-prints, and patents, ensuring reproducibility and accuracy in your research process.
It plays a crucial role in the body's immune response and fluid balance.
The peritoneum is composed of a single layer of squamous epithelial cells, known as mesothelial cells, which rest on a basement membrane.
This membrane provides a slippery surface that allows the abdominal organs to move freely during normal bodily functions.
Peritoneal research is essential for understanding various abdominal and pelvic conditions, such as peritonitis, ascites, and abdominal adhesions.
Optimize your peritoneum studies with the innovative PubCompare.ai tool, which can help you locate the best protocols from literature, pre-prints, and patents, ensuring reproducibility and accuracy in your research process.
Most cited protocols related to «Peritoneum»
Animals
Cytokine
Fear
Homo sapiens
Immunoassay
Immunoglobulins
Immunohistochemistry
Memory
Mice, House
Mice, Inbred C57BL
Microarray Analysis
Operative Surgical Procedures
Peritoneum
Pharmaceutical Preparations
Plasma
Plasma Proteins
prisma
Cell Lines
Cells
Collagenase
Liver
Lung
MUC1 protein, human
Mus
Neoplasm Metastasis
Neoplasms
Penicillins
Peritoneum
Streptomycin
Protocol full text hidden due to copyright restrictions
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ARID1A protein, human
Blood Vessel
Chemoradiotherapy
Dissection
Ethics Committees, Research
Neoplasms
Operative Surgical Procedures
Pathologists
Patients
Peritoneum
Radiosurgery
Surgeons
Surgical Margins
To establish FAEE-induced AP (FAEE-AP), adult CD1 mice received two intraperitoneal injections of ethanol (1.35 g/kg) and POA (150 mg/kg) at 1 h intervals. 200 µL normal saline was injected immediately prior to ethanol/POA injections to avoid potential local damage by ethanol to peritoneal organs at the injection site. Control adult CD1 mice received either saline, ethanol (1.35 g/kg) or POA (150 mg/kg). In treatment groups, mice also received 10 mg/kg 4-MP or 30 mg/kg 3-BCP simultaneously with the first injection of ethanol/POA. Animals were sacrificed at 24 h after the first injection. Histological assessment of damage was performed after H&E staining of fixed pancreatic slices (5 µm thickness); 10 random fields per slide from all animal groups were graded by two independent, blinded observers according to severity and extent of oedema, inflammatory cell infiltration and acinar necrosis as previously described.15 (link) Further details of FAEE-AP model characterisation are included in online supplementary materials and methods.
Adult
Animals
Cells
Edema
Ethanol
Inflammation
Injections, Intraperitoneal
Mice, House
Necrosis
Normal Saline
Pancreas
Peritoneum
Saline Solution
Buffers
cDNA Library
Cells
Genes
Genome
Macrophage
Macrophages, Alveolar
Mus
Oligonucleotide Primers
Peritoneum
Population Group
RNA, Messenger
RNA-Seq
Single-Cell RNA-Seq
Tromethamine
Most recents protocols related to «Peritoneum»
A pig was anesthetized with a mixture of alfaxalone (5 mg/kg), xylazine (2 mg/kg), and azaperone (6 mg/kg), and the following procedures were performed.
i) The 12-mm trocar was placed in the umbilicus of the pig and inflated with CO2 gas. Pneumoperitoneum was created and the intraabdominal pressure was maintained below 12 mmHg.
ii) Two additional ports were placed.
iii) The liver and gallbladder were identified under white light, and the gallbladder was pulled to the peritoneum.
iv) Five millimeters of SF solution was infused directly into the gallbladder. A small bile leak from the infused site was identified and clipped.
v) The biliary structures were observed under blue light emitted from an LED light source.
vi) The pig was euthanized at the end of the procedures.
Abdominal Cavity
alfaxalone
Azaperone
Gallbladder
Light
Liver
Peritoneum
Pneumoperitoneum
Pressure
Trocar
Umbilicus
Xylazine
All abdominal surgical approaches were performed using the laparoscopic ventral rectopexy method under general anesthesia regardless of the degree of rectal prolapse. All patients were placed in the lithotomy and Trendelenburg position after anesthesia, and a 12-mm trocar was inserted into the umbilicus for laparoscopic camera insertion, and four 5-mm trocars were inserted in each of the left and right upper and lower abdominal quadrants. The bowel was pulled out of the pelvis and the sigmoid colon was retracted to the left lateral side. The peritoneal opening was made in an inverted J-shape from the sacral cape to the left edge of the peritoneal reflex. The sterile polypropylene mesh (Prolene, Ethicon) was designed to have a length of 15 cm and a width of 2 cm. The mesh was properly positioned in the peritoneal opening, the lower end was sutured to the anterior wall of the rectum 2–3 cm from the edge of the anus, and the upper end was fixed to the right side of the periosteum of the sacral cape using ProTack (Covidien). The peritoneum opening was closed with continuous sutures using V-loc (Covidien) to prevent contact of the mesh with other organs in the abdomen.
Abdomen
Abdominal Cavity
Anesthesia
Anus
CM 2-3
General Anesthesia
Intestines
Laparoscopy
Operative Surgical Procedures
Patients
Pelvis
Periosteum
Peritoneum
Polypropylenes
Prolene
Rectal Prolapse
Rectum
Reflex
Sacrum
Sigmoid Colon
Sterility, Reproductive
Sutures
Trocar
Umbilicus
Filtered 1 mL of 10% protease peptone was injected intraperitoneally into the mice on the first day. The second injection was given after 12 h. Four hours later, peritoneal lavage cells were obtained by washing the abdominal cavity with RPMI-1640 supplemented with 10% FBS. After centrifugation, the cell pellets were resuspended and subjected to density gradient centrifugation with Percoll™ PLUS to obtain neutrophils. A flow cytometric assay was performed to detect the purity of the separated neutrophils (Supplement Figure 1 ). The purity of neutrophils = number of Ly6G-positive cells (neutrophils)/total number of living cells × 100%. After treatment, the cells were collected for Western blot analysis and other tests. In a previously published article, our laboratory specifically introduced the method of separating and extracting mouse peritoneal neutrophils34 .
Abdominal Cavity
Biological Assay
Cells
Centrifugation
Centrifugation, Density Gradient
Dietary Supplements
Flow Cytometry
Mus
Neutrophil
Pellets, Drug
Peptide Hydrolases
Peptones
Percoll
Peritoneal Lavage
Peritoneum
Western Blot
Neutrophils isolated from the peritoneal cells of mice were inoculated into 96-well plates (100 μL/well, 5 × 106 cells/well) with or without different concentrations of Sinomenine. The neutrophils were cultured at 37 °C in a 5% CO2 incubator. After four hours of culture, Cell Counting Kit 8 (CCK-8) solution (10 μL/well) was added to each well and incubated for 1 h. The absorbance at 450 nm was measured with a microplate reader (BioTek Instruments, Inc).
Cells
Mus
Neutrophil
Peritoneum
sinomenine
The peritoneal cavity was opened during isoflurane anesthesia, and the cecum was exteriorized. To induce light-grade CLP ~10-15% of the cecum was ligated using a nonabsorbable 7-0 suture (Johnson and Johnson, New Brunswick, NJ, USA). The distal end of the cecum was then perforated using a 23 G needle, and a small drop of feces was extruded through the puncture. The cecum was relocated into the peritoneal cavity and the peritoneum was closed using a nonabsorbable 5-0 suture (Johnson and Johnson). Animals were resuscitated by s.c. injection of 1 mL of saline and pain medication (Buprenorphin, 0.15 mg/kg) was injected sc. (23G Terumo, Leuven, Belgium). Mice were infected with HSV-1 after 7 days of sepsis.
Anesthesia
Animals
Cecum
Feces
Human Herpesvirus 1
Isoflurane
Light
Mice, House
Needles
Pain
Peritoneal Cavity
Peritoneum
Pharmaceutical Preparations
Punctures
Saline Solution
Sepsis
Sutures
Top products related to «Peritoneum»
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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
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Streptomycin is a broad-spectrum antibiotic used in laboratory settings. It functions as a protein synthesis inhibitor, targeting the 30S subunit of bacterial ribosomes, which plays a crucial role in the translation of genetic information into proteins. Streptomycin is commonly used in microbiological research and applications that require selective inhibition of bacterial growth.
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Penicillin is a type of antibiotic used in laboratory settings. It is a broad-spectrum antimicrobial agent effective against a variety of bacteria. Penicillin functions by disrupting the bacterial cell wall, leading to cell death.
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RPMI 1640 medium is a commonly used cell culture medium developed at Roswell Park Memorial Institute. It is a balanced salt solution that provides essential nutrients, vitamins, and amino acids to support the growth and maintenance of a variety of cell types in vitro.
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The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.
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The FACSCanto II is a flow cytometer instrument designed for multi-parameter analysis of single cells. It features a solid-state diode laser and up to four fluorescence detectors for simultaneous measurement of multiple cellular parameters.
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DMEM (Dulbecco's Modified Eagle's Medium) is a cell culture medium formulated to support the growth and maintenance of a variety of cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components necessary for cell proliferation and survival in an in vitro environment.
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RPMI 1640 is a common cell culture medium used for the in vitro cultivation of a variety of cells, including human and animal cells. It provides a balanced salt solution and a source of essential nutrients and growth factors to support cell growth and proliferation.
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The LPS laboratory equipment is a high-precision device used for various applications in scientific research and laboratory settings. It is designed to accurately measure and monitor specific parameters essential for various experimental procedures. The core function of the LPS is to provide reliable and consistent data collection, ensuring the integrity of research results. No further details or interpretations can be provided while maintaining an unbiased and factual approach.
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
More about "Peritoneum"
The peritoneum is a thin, smooth membrane that lines the abdominal cavity and covers the majority of the abdominal organs.
It plays a crucial role in the body's immune response and fluid balance.
The peritoneum is composed of a single layer of squamous epithelial cells, known as mesothelial cells, resting on a basement membrane.
This membrane provides a slippery surface that allows the abdominal organs to move freely during normal bodily functions.
Peritoneal research is essential for understanding various abdominal and pelvic conditions, such as peritonitis (inflammation of the peritoneum), ascites (fluid buildup in the abdominal cavity), and abdominal adhesions (scar tissue that binds organs together).
Understanding the peritoneum's structure and function is crucial for managing these conditions.
When conducting peritoneum studies, researchers may utilize various cell culture media and techniques to maintain and analyze peritoneal cells.
For example, RPMI 1640 medium, DMEM, and Penicillin/streptomycin are commonly used to culture peritoneal cells.
Flow cytometry techniques, such as FACSCalibur and FACSCanto II, can be employed to analyze the phenotype and function of peritoneal cells, including their immune response to stimuli like lipopolysaccharide (LPS).
Optimizing your peritoneum studies with the innovative PubCompare.ai tool can help you locate the best protocols from literature, pre-prints, and patents, ensuring reproducibility and accuracy in your research process.
This AI-driven platform can streamline your research by providing advanced comparisons and analyses, allowing you to make informed decisions and advance your understanding of the peritoneum and its role in various health and disease states.
It plays a crucial role in the body's immune response and fluid balance.
The peritoneum is composed of a single layer of squamous epithelial cells, known as mesothelial cells, resting on a basement membrane.
This membrane provides a slippery surface that allows the abdominal organs to move freely during normal bodily functions.
Peritoneal research is essential for understanding various abdominal and pelvic conditions, such as peritonitis (inflammation of the peritoneum), ascites (fluid buildup in the abdominal cavity), and abdominal adhesions (scar tissue that binds organs together).
Understanding the peritoneum's structure and function is crucial for managing these conditions.
When conducting peritoneum studies, researchers may utilize various cell culture media and techniques to maintain and analyze peritoneal cells.
For example, RPMI 1640 medium, DMEM, and Penicillin/streptomycin are commonly used to culture peritoneal cells.
Flow cytometry techniques, such as FACSCalibur and FACSCanto II, can be employed to analyze the phenotype and function of peritoneal cells, including their immune response to stimuli like lipopolysaccharide (LPS).
Optimizing your peritoneum studies with the innovative PubCompare.ai tool can help you locate the best protocols from literature, pre-prints, and patents, ensuring reproducibility and accuracy in your research process.
This AI-driven platform can streamline your research by providing advanced comparisons and analyses, allowing you to make informed decisions and advance your understanding of the peritoneum and its role in various health and disease states.