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> Anatomy > Tissue > Peritoneum, Parietal

Peritoneum, Parietal

The parietal peritoneum is the portion of the peritoneum lining the abdominal wall.
It plays a key role in the body's immune response and fluid balance.
Disorders of the parietal peritoneum can include inflammation, infection, and damage from trauma or surgery.
Researchers studying the parietal peritoneum may need to optimize and reproducre their research protocols to gain insights into its functions and potential therapeutic targets.
The PubCompare.ai platform can help locate relevant protocols from the literature, preprints, and patents, and leverage AI-driven comparisons to identify the best approaches for your research needs.
Take your work on the parietal peritoneum to new heights with this powerful tool.

Most cited protocols related to «Peritoneum, Parietal»

1
Evaluating Ovarian Cancer Cell Adhesion to Peritoneal Mesothelium
To evaluate adhesion to intact peritoneal mesothelium, an ex vivo peritoneal explant assay was utilized [32 (link),33 (link)]. An optically clear solid support was prepared using Sylgard® 184 silicone elastomer kit (Fisher) according to manufacturer’s specifications. Mice were first euthanized by CO2 inhalation followed by cervical dislocation, then rapidly dissected using a ventral midline incision. After skin removal, the parietal peritoneum lining the ventral abdominal wall was dissected to remove a ~ 1.2×1.2 cm2 square of peritoneal tissue immediately adjacent to the midline in the lower two abdominal quadrants. The tissue explant was then pinned mesothelial-side up to the silastic resin using fine gauge dissecting pin and the explant immersed in PBS. Tissue integrity is maintained for up to 48 h. Fluorescently tagged ovarian cancer cells (2.5 × 105 cells/ml) were added to the explant in culture medium (2.5 ml) and allowed to adhere for 0.5–2 h prior to gently washing and processing the explant for fluorescence microscopy using an EVOS FL microscope or scanning electron microscopy (below).
Liu Y., Metzinger M.N., Lewellen K.A., Cripps S.N., Carey K.D., Harper E.I., Shi Z., Tarwater L., Grisoli A., Lee E., Slusarz A., Yang J., Loughran E.A., Conley K., Johnson J.J., Klymenko Y., Bruney L., Liang Z., Dovichi N.J., Cheatham B., Leevy W.M, & Stack M.S. (2015). Obesity Contributes to Ovarian Cancer Metastatic Success Through Increased Lipogenesis, Enhanced Vascularity, and Decreased Infiltration of M1 Macrophages. Cancer research, 75(23), 5046-5057.
Publication 2015
Abdomen Biological Assay Cells Culture Media Inhalation Joint Dislocations Mesothelium Microscopy Microscopy, Fluorescence Mus Neck Ovarian Neoplasm Peritoneum Peritoneum, Parietal Resins, Plant Scanning Electron Microscopy Silastic Silicone Elastomers Skin Tissues Wall, Abdominal
2
Peritoneal Fibrosis Treatment with 3-DZNeP
Male C57/black mice (Shanghai Super-B&K Laboratory Animal Corp. Ltd) that weighed 20–25g were housed under a 12:12-h light-dark cycle with food and water supplied ad libitum. Two mouse models of peritoneal fibrosis were established. The first peritoneal fibrosis model was created by daily intraperitoneal injection of 100ml/kg peritoneal dialysis solution with 4.25% glucose for 28 days [10 (link)]. The second peritoneal fibrosis model was established by intraperitoneally injection of 0.1% chlorhexidine gluconate (CG) dissolved in saline every other day for 21 days [28 (link),29 (link)]. To investigate the effect of 3-DZNeP in peritoneal fibrosis, mice were injected intraperitoneally with a single dose of 3-DZNeP (1 mg/kg) in DMSO every day. Mice were randomly divided the mice into four groups in each model: (1) mice injected with an equivalent amount of saline intraperitoneally and DMSO (n=6), defined as the sham group; (2) mice injected an equivalent amount of saline intraperitoneally and 3-DZNeP (n=6), defined as sham + 3-DZNeP group; (3) mice injected PDF or CG intraperitoneally and DMSO (n=6), defined as PDF/CG group; and (4) mice injected PDF or CG intraperitoneally and 3-DZNeP, defined as PDF/CG + 3-DZNeP group (n=6). All animals were sacrificed, and parietal peritoneum was collected from each mouse for further protein analysis and histological examination at the end of 28 days in the PDF model or 21 days in the CG model. To examine the therapeutic its effect on established peritoneal fibrosis, 3-DZNeP was administered starting at 28 days in mice after injection with PDF and then given daily for 14 days. At the end of 42 days, all mice were euthanized for collection of peritoneal tissue. All the experiments were conducted in accordance with the animal experimentation guidelines of Tongji University School of Medicine, China.
Shi Y., Tao M., Wang Y., Zang X., Ma X., Qiu A., Zhuang S, & Liu N. (2019). Genetic or pharmacologic blockade of enhancer of zeste homolog 2 inhibits the progression of peritoneal fibrosis. The Journal of pathology, 250(1), 79-94.
Publication 2019
3-deazaneplanocin Animals Animals, Laboratory chlorhexidine gluconate Dialysis Solutions Food Glucose Injections, Intraperitoneal Males Mice, House Peritoneal Dialysis Peritoneal Fibrosis Peritoneum Peritoneum, Parietal Pharmaceutical Preparations Proteins Saline Solution Sulfoxide, Dimethyl Therapeutics
3
Inducing Peritoneal Fibrosis in Mice
The peritoneal fibrosis model was established in C57/BL6 mice weighing 20-25 g (Shanghai Super-B&K Laboratory Animal Corp. Ltd.). Peritoneal fibrosis in mice was created by intraperitoneal injection of 3 ml peritoneal dialysis solution with 4.25% glucose (Baxter) every day for 28 days [1 (link)]. To investigate the effect of HDAC6 inhibition on peritoneal fibrosis, 70 mg/kg TA was delivered by daily intraperitoneal injection in DMSO vehicle. Mice were randomly divided into four groups with 6 mice per group: the sham group with DMSO, sham administered TA group, the peritoneal fibrosis group and mice with peritoneal fibrosis administered TA. At the end of 28 days, all mice were sacrificed and the parietal peritoneum was collected for further analysis. All the experiments were conducted in accordance with the animal experimentation guideline of Tongji University School of Medicine, China.
Xu L., Liu N., Gu H., Wang H., Shi Y., Ma X., Ma S., Ni J., Tao M., Qiu A, & Zhuang S. (2017). Histone deacetylase 6 inhibition counteracts the epithelial–mesenchymal transition of peritoneal mesothelial cells and prevents peritoneal fibrosis. Oncotarget, 8(51), 88730-88750.
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Publication 2017
Animals, Laboratory Dialysis Solutions Glucose Injections, Intraperitoneal Mice, House Peritoneal Dialysis Peritoneal Fibrosis Peritoneum Peritoneum, Parietal Pharmaceutical Preparations Psychological Inhibition Sulfoxide, Dimethyl
4
Peritoneal Membrane Histological Analysis
Parietal peritoneal tissues were biopsied in the standard manner and processed as reported previously [22 (link),23 (link)]. Parietal peritoneal membrane was obtained from the anterior abdominal wall when the PD catheter was removed at the cessation of PD. To avoid detachment of mesothelial cells, all procedures were carried out carefully. Peritoneal membrane tissues fixed with formalin were stained with hematoxylin and eosin (HE) and Masson’s trichrome [23 (link)–26 (link)] and were also stained with phosphotungstic acid hematoxylin (PTAH) reagent to detect fibrin formation, as described previously [27 (link)]. Immunostaining was performed on paraffin-embedded tissues as described previously [23 (link)–25 (link),28 (link)]. The antibodies used in these experiments are summarized in S1 Table. Briefly, 4-μm-thick sections of formalin-fixed, paraffin-embedded tissues were dewaxed and rehydrated. Endogenous peroxidase activity was inhibited with 3% H2O2 in methanol. For antigen retrieval to detect CD31 and CD68, the slides were boiled in a solution of 0.04 M citrate and 0.12 M phosphate (pH 5.8) for 30 min at 98°C. After washing, nonspecific protein-binding sites were blocked with normal goat serum (Dako, Glostrup, Denmark). Then, sections were incubated with primary antibodies, mouse monoclonal antibodies against CD31 (JC/70A; Dako), CD68 (PGM1; Dako), and podoplanin (D2-40; Dako) overnight at 4°C. For advanced glycation end-products (AGEs), sections were incubated with mouse anti-AGE antibody (6D12; TransGenic, Kobe, Japan) for 60 min at room temperature. After washing with phosphate-buffered saline, sections were treated with a conjugate of polyclonal goat anti-mouse immunoglobulin (Ig) G antibodies and horseradish peroxidase-labeled polymer (Histofine® Simple Stain; Nichirei, Tokyo, Japan) as the secondary reagent. Enzyme activity was detected by 3,3'-diaminobenzidine (Nichirei). For analysis of collagen volume fraction (collagen density), we applied the methods described by Morelle et al. [29 (link)]. Briefly, we stained peritoneal membrane tissues with a Picrosirius Red Stain kit (Polyscience, Warrington, PA), then observed the tissues under circularly polarized light microscopy (Zeiss Z1 image microscopy, Carl Zeiss, Oberkochen, Germany). Collagen volume fraction was assessed using ImageJ software version 1.5 (http://imagej.nih.gov/ij/) and was calculated as a percentage of the submesothelial area.
Tawada M., Ito Y., Hamada C., Honda K., Mizuno M., Suzuki Y., Sakata F., Terabayashi T., Matsukawa Y., Maruyama S., Imai E., Matsuo S, & Takei Y. (2016). Vascular Endothelial Cell Injury Is an Important Factor in the Development of Encapsulating Peritoneal Sclerosis in Long-Term Peritoneal Dialysis Patients. PLoS ONE, 11(4), e0154644.
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Publication 2016
Animals, Transgenic Anti-Antibodies Antibodies Antibodies, Anti-Idiotypic Antigens Catheters Cells Citrates Collagen enzyme activity Eosin Fibrin Formalin Glycation End Products, Advanced Goat Horseradish Peroxidase Immunoglobulin G Mesothelium Methanol Microscopy Microscopy, Polarization Monoclonal Antibodies Mus Paraffin Embedding Peritoneum Peritoneum, Parietal Peroxidase Peroxide, Hydrogen Phosphates Phosphotungstic Acid Polymers Saline Solution Serum Stains Tissue, Membrane Tissues Wall, Abdominal
5
Optimized Peritoneal Adhesion Model
General anesthesia was achieved using a weight depending dose of 80 mg/kg body weight Ketamine and 5 mg/kg Xylazine®. Required level of narcosis for surgery was reached if flexor reflexes failed to appear. The abdomen was then shaved and prepared with alcohol and iodine solution. A 4 cm median laparotomy was performed to gain access to the abdominal cavity. In the optimized peritoneal adhesion model group (OPAM) (n = 10), the cecum was delivered and kept moist with a watery gauze swab whilst dry gauze was used to gently abrade the cecal peritoneum in a standard manner. Abrasion was repeated until removal of visceral peritoneum, occurring of sub-serosal bleeding, and creation of a homogenous surface of petechial hemorrhages over a 1 x 2 cm area. An 1 x 2 cm sized patch of parietal peritoneum with the underlying inner muscular layer was sharply resected of the right-lateral abdominal wall (Figure 1A). After replacing the cecum intra-abdominally, both defects were approximated with a 4/0 Prolene® suture to fix the mesentery of the ascending colon to the abdominal wall (Figure 1B). The group without suture fixation (WSFX) (n = 4) represents conventional adhesion models as cecum and abdominal wall, exactly injured as described above, were not approximated. In the sham-OPAM group (n = 5) only the approximating suture was placed without peritoneal injuries. In sham-WSFX group (n = 5) animals underwent only laparotomy without any injury and/or suturing. The abdomen was closed using two-layer closure technique by a consecutive suture.
Poehnert D., Abbas M., Kreipe H.H., Klempnauer J, & Winny M. (2015). High Reproducibility of Adhesion Formation in Rat with Meso-Stitch Approximation of Injured Cecum and Abdominal Wall. International Journal of Medical Sciences, 12(1), 1-6.
Publication 2015
Abdominal Cavity Animals Bladder Detrusor Muscle Body Weight Cecum Ethanol General Anesthesia Hemorrhage Homozygote Injuries Iodine Ketamine Laparotomy Mesocolon Narcosis Operative Surgical Procedures Peritoneum Peritoneum, Parietal Petechiae Prolene Reflex Serous Membrane Suture Techniques Visceral Peritoneum Wall, Abdominal Xylazine

Most recents protocols related to «Peritoneum, Parietal»

1
Minimizing Incisional Hernia in Colon Cancer Laparoscopy
A small incision will be made by extending the periumbilical port for the camera scope in both groups. The size of the small incision will be determined based on the size of the tumour and the physical habits of the patient. The fascial closure methods are standardised as continuous closure using Stratafix (SF Symmetric PDS Plus®) with a 4:1 ratio (4-to-1 suture to wound length ratio) and bites of < 1 cm. The methods for closure of the subcutaneous fat and skin (skin stapler or 3–0 nylon vertical mattress) depend on the surgeon’s discretion. Patients randomly assigned to the midline group will undergo an incision along the midline skin, subcutaneous fat, and linea alba.
In the non-muscle-cutting periumbilical transverse group, the method of small incision is the same as in a previous report (Fig. 2) [6 (link)]. Briefly, the skin incision of the 11-mm periumbilical port will be extended transversely. Using monopolar electrocautery and crossing linea alba, the anterior and posterior rectus sheaths are transversely incised. With lateral traction of the rectus abdominis muscle with an army retractor, the posterior rectus sheath can be seen (Supplemental Video 1). The transversalis fascia and parietal peritoneum are further incised transversely. Continuous fascia closure will be separately performed for the anterior and posterior rectus sheaths. Implementing vertical or transverse incisions will not require alteration to usual care pathways (including the use of any medication), and these will continue for both trial arms.

A Transverse skin incision. B Transverse incision of the anterior fascia of the rectus abdominis muscle. C Transverse incision of the posterior fascia of the rectus abdominis muscle. D Incision completed. Note. This figure was produced by Chang Hyun Kim in 2022. From “Periumbilical Transverse Incision for Reducing Incisional Hernia in Laparoscopic Colon Cancer Surgery,” by Chang Hyun Kim et al., 2022, World Journal of Surgery,46(4): p918. Copyright 2022 by SPRINGER

Park S.Y., Ha G.W., Lee S.Y., Kim C.H, & Son G.M. (2023). Impact of non-muscle cutting periumbilical transverse incision on the risk of incisional hernia as compared to midline incision during laparoscopic colon cancer surgery: a study protocol for a multi-centre randomised controlled trial. Trials, 24, 152.
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Publication 2023
Arm, Upper Bites Cancer of Colon Electrocoagulation Fascia Incisional Hernia Muscle Tissue Myotomy Neoplasms Nylons Operative Surgical Procedures Patients Peritoneum, Parietal Pharmaceutical Preparations Physical Examination Rectus Abdominis Skin Subcutaneous Fat Surgeons Surgical Procedures, Laparoscopic Sutures Traction Vision Wounds
2
Abdominal Imaging Indexes for Pancreatic Surgery

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Open the protocol to access the free full text link

Publication 2023
Abdomen Abdominal Cavity MECA-79 antigen Neck Operative Surgical Procedures Pancreas Pancreatic Duct Patients Peritoneum, Parietal Skin Subcutaneous Fat Surgeons Vein, Mesenteric Veins, Portal Veins, Splenic Wall, Abdominal
3
Peritoneal Surface Sampling Protocol
During the CS realisation each cow was sampled twice. The samples were collected just after replacing the sutured uterus in the abdominal cavity before the closure of the abdominal wall. At this moment, swabs (STERILER®, Piove di Sacco, Italy) were taken by swiping a long line of 10 cm, 2 cm in parallel to the uterus suture (visceral peritoneum of the uterus) and a 10 cm long line perpendicular to and below the abdominal wall incision (parietal peritoneum).
Djebala S., Coria E., Munaut F., Gille L., Eppe J., Moula N., Taminiau B., Daube G, & Bossaert P. (2023). Bacterial Contamination of the Surgical Site at the Time of Elective Caesarean Section in Belgian Blue Cows—Part 2: Identified by 16Sr DNA Amplicon Sequencing. Veterinary Sciences, 10(2), 94.
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Publication 2023
Abdominal Cavity Peritoneum, Parietal Sutures Uterus Visceral Peritoneum Wall, Abdominal
4
Abdominal Adipose Tissue Measurement
The abdominal adipose tissue areas were measured at the level of the umbilicus using a 16-detector row CT scanner (Somatom Sensation 16, Siemens Medical Solutions, Forchheim, Germany), as previously described [22 (link)]. In brief, a 5-mm-thick umbilical-level abdominal section was obtained. The cross-sectional area (cm2) of the abdominal adipose tissue was calculated using Rapidia 2.8 CT software (INFINITT, Seoul, Korea). The VAT area was defined as intra-peritoneal fat bound by the parietal peritoneum or transversalis fascia, and the SAT area was defined as fat areas external to the abdomen and back muscles. The total adipose tissue (TAT) area was calculated based on the summation of VAT and SAT. Because a clear standard for determining the normal abdominal fat amount has not been established, we used the lowest quartile as the reference group after subdividing abdominal fat amounts by quartile. In this study, we defined central obesity as increased VAT above the 75th percentile.
Lee J., Min S., Oh S.W., Oh S., Lee Y.H., Kwon H., Lee C.M., Choi H.C, & Heo N.J. (2023). Association of intraabdominal fat with the risk of incident chronic kidney disease according to body mass index among Korean adults. PLOS ONE, 18(2), e0280766.
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Publication 2023
Abdomen Abdominal Cavity Abdominal Fat CAT SCANNERS X RAY Fascia Muscle, Back Peritoneum Peritoneum, Parietal Tissue, Adipose Umbilicus
5
Histopathological Analysis of Parietal Peritoneum
Parietal peritoneal tissues were fixed in 10% neutral formalin and embedded in paraffin to obtain 3–4-μm-thick serial tissue sections. Deparaffinized sections were stained with hematoxylin and eosin (H&E) and Masson’s trichrome solution to analyze histopathological characteristics. Before immunohistochemistry, tissue sections were heated using citrate buffer (pH 6.0) to unmask antigens. Samples were pretreated with a 3% hydrogen peroxide solution to block endogenous peroxidase activity. Secondary goat anti-rat or anti-rabbit antibodies were applied as appropriate to detect the primary antibody. Primary antibodies were incubated overnight at 4 °C in a humidity chamber after blocking the slides for 30 min with 3% BSA. DAB (Dako) was used as an HRP substrate for signal detection [28 (link)].
Wang Q., Sun J., Wang R, & Sun J. (2023). Inhibition of EZH2 mitigates peritoneal fibrosis and lipid precipitation in peritoneal mesothelial cells mediated by klotho. Renal Failure, 45(1), 2149411.
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Publication 2023
Anti-Antibodies Antibodies Antigens Buffers Citrate Eosin Formalin Goat Humidity Immunoglobulins Immunohistochemistry Paraffin Embedding Peritoneum, Parietal Peroxidase Peroxide, Hydrogen Rabbits Signal Detection (Psychology) Tissues

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Somatom sensation 16 by Siemens
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2
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More about "Peritoneum, Parietal"

The peritoneum is a thin, transparent membrane that lines the abdominal cavity and covers most of the abdominal organs.
It is composed of two main parts: the parietal peritoneum and the visceral peritoneum.
The parietal peritoneum is the portion that lines the abdominal wall, while the visceral peritoneum covers the organs within the abdomen.
The parietal peritoneum plays a crucial role in the body's immune response and fluid balance.
It is a semi-permeable membrane that allows the exchange of fluids and substances between the abdominal cavity and the bloodstream.
Disorders of the parietal peritoneum can include inflammation (peritonitis), infection, and damage from trauma or surgery.
Researchers studying the parietal peritoneum may need to optimize and reproduce their research protocols to gain insights into its functions and potential therapeutic targets.
This can involve the use of various tools and techniques, such as the Somatom Sensation 16 CT scanner, the Brilliance 64 imaging system, and the FV1000 confocal microscope.
The Anti-CD88 Alexa Fluor 594 antibody and the 565DCXR dye may be used to label and visualize specific cells or structures within the parietal peritoneum.
Additionally, the Sylgard 184 Silicone Elastomer Kit may be used to create custom-made experimental models or devices for studying the parietal peritoneum.
The DAB (3,3'-Diaminobenzidine) chromogen can be used for immunohistochemical staining to identify and localize specific proteins or antigens within the parietal peritoneal tissue.
The PubCompare.ai platform can be a valuable tool for researchers studying the parietal peritoneum.
This AI-driven platform can help locate relevant protocols from the literature, preprints, and patents, and leverage AI-driven comparisons to identify the best approaches for your research needs.
By utilizing PubCompare.ai, researchers can take their work on the parietal peritoneum to new heights and uncover valuable insights into its functions and potential therapeutic applications.