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> Anatomy > Tissue > Serous Membrane

Serous Membrane

Serous Membranes: Thin, delicate layers of tissue that line various cavities within the body and cover the organs they contain.
Theses membranes secrete a lubricating serous fluid, allowing for smooth movement and protection of the enclosed structures.
Serous membranes play a crucial role in the normal functioning of the body's organ systems, such as the cardiovascular, respiratory, and reproductive systems.
Understanding the structure and function of serous membranes is essential for medical research and clinical practice, particularly in the areas of disease diagnosis, treatment, and prevention.
Explore PubCompare.ai's AI-driven platform to discover optimized protocols, compare products, and unlock the potential of Seorus Membrane studies.

Most cited protocols related to «Serous Membrane»

1
Colon Histopathological Scoring Protocol
Following euthanasia, mouse colons were fixed in 10% buffered formalin for 24 hours at room temperature and then embedded in paraffin. Tissues were sectioned at 5-µm thickness and stained with hematoxylin & eosin (H&E) using standard protocols. H&E stained slides were scored by two pathologists (A.N.Y. and M.A.D.). Each colon was assigned four scores based on the degree of epithelial damage and inflammatory infiltrate in the mucosa, submucosa and muscularis/serosa, as previously described [25] (link). A slight modification was made to this scoring system; each of the four scores was multiplied by 1 if the change was focal, 2 if it was patchy and 3 if it was diffuse. The 4 individual scores per colon were added, resulting in a total scoring range of 0–36 per mouse. The scores for each of five mice per treatment group (i.e., exposed to 0, 0.25, 0.5, 1.0 and 4.0% DSS) were averaged.
Chassaing B., Srinivasan G., Delgado M.A., Young A.N., Gewirtz A.T, & Vijay-Kumar M. (2012). Fecal Lipocalin 2, a Sensitive and Broadly Dynamic Non-Invasive Biomarker for Intestinal Inflammation. PLoS ONE, 7(9), e44328.
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Publication 2012
Bladder Detrusor Muscle Colon Eosin Euthanasia Formalin Mucositis Mus Paraffin Embedding Pathologists Serous Membrane Tissues
2
Intestinal Inflammation Scoring Protocol
Formalin-fixed and paraffin-embedded intestinal tissue was sectioned and stained with hematoxylin and eosin (H&E) as previously described4 (link). A semi-quantitative composite scoring system was used for the assessment of spontaneous intestinal inflammation, computed as a sum of five histological subscores, multiplied by a factor based on the extent of the inflammation. Histological subscores (for each parameter: 0, absent; 1, mild; 2, moderate; 3, severe): mononuclear cell infiltrate (0-3), crypt hyperplasia (0-3), epithelial injury/erosion (0-3), polymorphonuclear cell infiltrates (0-3) and transmural inflammation (0, absent; 1, submucosal; 2, one focus extending into muscularis and serosa; 3 up to five foci extending into muscularis and serosa; 4, diffuse). Extent factor was derived according to the fraction of bowel length involved by inflammation: 1, < 10%, 2, 10-25%, 3, 25-50% and 4, >50%. Ileal inflammation was assessed by an expert gastrointestinal pathologist (J.N.G.) who was blinded to the genotype and experimental conditions of the samples. No spontaneous colonic inflammation was detected in any of the reported genotypes.
Adolph T.E., Tomczak M.F., Niederreiter L., Ko H.J., Böck J., Martinez-Naves E., Glickman J.N., Tschurtschenthaler M., Hartwig J., Hosomi S., Flak M.B., Cusick J.L., Kohno K., Iwawaki T., Billmann-Born S., Raine T., Bharti R., Lucius R., Kweon M.N., Marciniak S.J., Choi A., Hagen S.J., Schreiber S., Rosenstiel P., Kaser A, & Blumberg R.S. (2013). Paneth cells as a site of origin for intestinal inflammation. Nature, 503(7475), 272-276.
Publication 2013
Bladder Detrusor Muscle Cells CFC1 protein, human Colon Eosin factor A Formalin Genotype Granulocyte Hyperplasia Ileum Inflammation Injuries Intestines Paraffin Pathologists Serous Membrane Tissues
3
Comparative Evaluation of Digital Filters for Gastric Electrophysiology
Ethical approval was granted by our institutional and national review panels. Digital filters were evaluated on raw unipolar recordings acquired using the ActiveTwo system (Biosemi, The Netherlands), at a sampling frequency of 512 Hz. The data acquisition was performed using a large dynamic range (24 bit delta-sigma analog to digital convertor, resolution 31.2 nV) with no high pass filtering, and a low pass filter by the ADC’s decimation filter due to hardware bandwidth limitations (effective bandwidth from DC (0Hz) to 400Hz at -3dB). Recordings were taken from the gastric serosa of a pig and human using flexible arrays (16 (link)) according to our previously published methods (2 (link), 3 (link)), and ten representative data segments were analyzed (855 s for pig, 500 s for human).
Four different filters with distinct specifications were identified from recent literature for comparison: Bandpass FIR (Finite impulse response) filter (0.05–1 Hz) (17 (link), 18 (link)); SG (Savitzky-Golay) filter (low pass filter with cutoff frequency of 1.98 Hz) (9 (link), 13 (link)); Bandpass Bessel filter (2–100 Hz) (15 (link)), and a Bandpass Butterworth filter (5–100 Hz) (15 (link)). These four filters were applied after the removal of baseline wander (via a moving median window of 20 seconds (9 (link))) and notch filters to remove power line interference for consistent comparison. Data processing and analysis was performed in MATLAB v7.11 (Natick, Massachusetts).
After filtering, the resultant signals were evaluated in both the time and frequency domains. Two measures were used to quantify the filter effects: average slow wave amplitude in the time domain, and maximum spectral component in the frequency domain (computed via the Fourier transform). Amplitude in the time domain was computed by the difference between the minimum and maximum of a running window of two minutes and averaged. In the frequency domain, the spectral component with the highest amplitude was acquired. For statistical analyses, t-tests were performed between the amplitude and frequency of the baseline removed signal, and the filtered signals.
Paskaranandavadivel N., O’Grady G., Du P, & Cheng L.K. (2012). Comparison of Filtering Methods for Extracellular Gastric Slow Wave Recordings. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, 25(1), 79-83.
Publication 2012
Fingers Homo sapiens Neoplasm Metastasis Serous Membrane Stomach
4
Decellularization of Porcine Tissues

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Publication 2012
Animals Bath Brain Cells Connective Tissue Decellularized Extracellular Matrix Deoxycholate Edetic Acid Ethanol Freeze Drying Freezing Membrane, Basement Muscularis Mucosae Peracetic Acid Pigs Serous Membrane Spinal Cord Sucrose Tissues Tissue Specificity Triton X-100 Trypsin Urinary Bladder Urothelium
5
Isolation of Intestinal Cell Subtypes

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Publication 2009
Bladder Detrusor Muscle Cecum Cells Colon Digestion Dissection Edetic Acid Epithelial Cells Flow Cytometry Hemoglobin, Sickle Ileum Intestines Intestines, Small Jejunum Large Intestine Matrix Metalloproteinase 2 Mesentery Microscopy Monoclonal Antibodies Mucous Membrane Serous Membrane Spleen Surgical Clamps Tissues

Most recents protocols related to «Serous Membrane»

1
5HTR4 Receptor Role in Tryptamine Signaling
Not available on PMC !

Example 5

The response to 5-hydroxytryptophan (5HT; 0.003-300 μM) on the mucosal or serosal side and tryptamine (0.003-3000 μM) on the mucosal or serosal side was determined in segments of proximal colon, stripped of external muscle layers, from both 5HTR4 KO and WT mice.

Colon segments from 5HTR4 KO mice displayed decreased responsiveness to serosal serotonin and no response to mucosal serotonin when compared with colon segments from WT mice. Cumulative concentration response curves induced by serosal tryptamine were significantly different between 5HTR WT (Emax: 110±17 μA/cm2; n=6-7) and KO mice (no response). While Δlsc did not reach maximum response following mucosal application of 3000 μM tryptamine, responses were seen in 5HTR4 WT mice (99.5±30.7 n=5) while no response was elicited in 5HTR4 KO (FIG. 18).

These results show that tryptamine acts as a 5HTR4 mimetic with effects on gut epithelial function independent of serotonin.

US11878002B2. Methods and materials for using Ruminococcus gnavus or Clostridium sporogenes to treat gastrointestinal disorders (2024-01-23). Mayo Foundation for Medical Education and Research [US], The Regents of the University of California [US]. Inventors: Purna C. Kashyap [US], Michael Fischbach [US], Brianna B. Williams [US].
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Patent 2024
5-Hydroxytryptophan Bladder Detrusor Muscle Colon Mice, Knockout Mucous Membrane Mus Serotonin Serous Membrane SERPINA3 protein, human tryptamine Vision
2
Ussing Chamber Assay for Intestinal Permeability
Intestinal permeability was determined using Ussing chamber analysis. The colonic mucosa was intactly scraped from the distal colon specimens, installed in a slider with a 0.3 cm2 rectangular hole in the center, fixed in the U-shaped chamber, and immersed in oxygen-containing Krebs’ solution on both the serosal and mucosal sides. Then, the chamber was mounted on Ussing Chamber System (World Precision Instruments, USA). The transepithelial resistance (TER) of the colonic mucosa was recorded by an automatic voltage clamp model after a 20 min equilibration. In addition, mucosal-to-serosal permeability was assessed by fluorescein isothiocyanate conjugated dextran (FD4, FITC-dextran, molecular weight: 4 kD, Sigma-Aldrich, Madrid, Spain). After the TER recording, 1 mg/ml FD4 was added to the mucosal side of the chamber, and the same volume of Krebs’ solution was added to the serosal side without light. One hundred microliters of solution was sampled from the serosal side every 30 min over a 2 h period, and the fluorescence intensity was detected by a fluorescence spectrophotometer (485 nm/528 nm, Ex/Em, BioTek, Winooski, VT, USA). The FD4 concentration in the serosal side was evaluated by a standard curve of continuous dilutions of FD4 in Krebs’ solution.
Yang J., Wang L., Mei M., Guo J., Yang X, & Liu S. (2023). Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis. Chinese Medicine, 18, 24.
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Publication 2023
Colon fluorescein isothiocyanate dextran Fluorescence Intestines Krebs-Ringer solution Light Mucous Membrane Oxygen Permeability Serous Membrane Technique, Dilution
3
Histological Evaluation of Colon Tissue Damage
Colon tissues were fixed in 10% neutral buffered formalin (G2161, Solarbio, Beijing, China) at 4 °C for 24 h, dehydrated, immersed in wax, embedded, and then sliced. The slices were deparaffinized using xylene for 5–10 min, and subsequently deparaffinized for another 5–10 min with fresh xylene. Next, the slices were subjected to treatment with absolute ethanol for 5 min, 90% ethanol for 2 min, 80% ethanol for 2 min, 70% ethanol for 2 min, distilled water for 2 min, and stained with the hematoxylin staining solution for 5–10 min. The excess staining solution was rinsed with deionized water for 10 min. Next, slices were stained with the eosin staining solution for 30 s–2 min. The slices were then dehydrated with 70% ethanol for 10 s, 80% ethanol for 10 s, 90% ethanol for 10 s, and absolute ethanol for 10 s, cleared using xylene for 5 min, and again for another 5 min with fresh xylene. Subsequently, slices were mounted with neutral gum, while the observations were documented under an inverted microscope (IX73, Olympus, Tokyo, Japan).
According to the criteria of Dieleman, the severity of the lesion was scored based on the depth and extent of the ulcer as well as the degree of inflammation, and the depth of the lesion, the scoring was as follows: 0 points, no disease; 1 point, the invasion of salina layer by the lesion; 2 points, invasion of the sub-anvil membrane by the lesion; 3 points, invasion of the muscle layer by the lesion; 4 points, invasion of the serosal layer by the lesion. The extent of lesions was scored as follows: 0 points, no lesions. 1 point, 0–25%; 2 points, 26–50%; 3 points: 51–75%; 4 points, >75%. The degree of inflammation was scored as follows: 0 points, no inflammation; 1 point, mild inflammation; 2 points, moderate inflammation; 3 points, severe inflammation.
Ning L., Ye N., Ye B., Miao Z., Cao T., Lu W., Xu D., Tan C., Xu Y, & Yan J. (2023). Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarization. Cell Death Discovery, 9, 84.
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Publication 2023
Bladder Detrusor Muscle Colon Eosin Ethanol Formalin Inflammation Microscopy Serous Membrane Tissue, Membrane Tissues Ulcer Xylene
4
Localization Accuracy: Endoscopic vs. Colonoscopy
The primary objective is to compare the localization accuracy of preoperative endoscopic localization with autologous blood versus intraoperative colonoscopy localization for small, serosa-negative lesion which will be resected by laparoscopic colectomy. The secondary objective is adverse events related to endoscopic tattooing.
Zhang K.H., Li J.Z., Zhang H.B., Hu R.H., Cui X.M., Du T., Zheng L., Zhang S., Song C., Xu M.D, & Jiang X.H. (2023). Assessment of Autologous Blood marker localIzation and intraoperative coLonoscopy localIzation in laparoscopic colorecTal cancer surgery (ABILITY): a randomized controlled trial. BMC Cancer, 23, 204.
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Publication 2023
BLOOD Colectomy Colonoscopy Endoscopy Laparoscopy Serous Membrane
5
Localization of Small Colonic Lesions
Preoperative endoscopic localization with autologous blood is no inferiority to intraoperative colonoscopy localization in small, serosa-negative lesion which will be performed by laparoscopic colectomy.
Zhang K.H., Li J.Z., Zhang H.B., Hu R.H., Cui X.M., Du T., Zheng L., Zhang S., Song C., Xu M.D, & Jiang X.H. (2023). Assessment of Autologous Blood marker localIzation and intraoperative coLonoscopy localIzation in laparoscopic colorecTal cancer surgery (ABILITY): a randomized controlled trial. BMC Cancer, 23, 204.
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Publication 2023
BLOOD Colectomy Colonoscopy Endoscopy Laparoscopy Serous Membrane

Top products related to «Serous Membrane»

1
Ussing chamber by Physiologic Instruments
Sourced in United States
Ussing chambers are specialized laboratory equipment used to measure the transepithelial transport of ions, solutes, and water across epithelial cell layers. They provide a controlled environment for the study of physiological processes in biological membranes.
2
Fitc dextran by Merck Group
Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, Japan, China, Macao, Spain, Switzerland, France, Italy, Australia, Israel, Sweden, Belgium, Canada
FITC-dextran is a fluorescent labeled dextran compound. It is a water-soluble carbohydrate polymer that is covalently linked to the fluorescent dye fluorescein isothiocyanate (FITC). FITC-dextran is commonly used as a tracer or marker in various biological applications.
3
Ussing chamber by Harvard Apparatus
Sourced in United States
Ussing chambers are specialized laboratory equipment used for the study of ion and molecule transport across biological membranes. They provide a controlled environment to measure the movement of substances through epithelial or endothelial cell layers.
4
Matrigel by BD
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Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins. It is widely used as a substrate for the in vitro cultivation of cells, particularly those that require a more physiologically relevant microenvironment for growth and differentiation.
5
Forskolin by Merck Group
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Forskolin is a lab equipment product manufactured by Merck Group. It is a compound derived from the roots of the Coleus forskohlii plant. Forskolin is used as a tool for research purposes in the laboratory setting.
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Carbachol by Merck Group
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Carbachol is a chemical compound that acts as an acetylcholine receptor agonist. It is commonly used in laboratory settings as a research tool to study the effects of acetylcholine on various biological systems.
7
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Matrigel is a complex mixture of extracellular matrix proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is widely used as a basement membrane matrix to support the growth, differentiation, and morphogenesis of various cell types in cell culture applications.
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Image pro plus 4 by Media Cybernetics
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Image-Pro Plus 4.5 is a comprehensive image analysis software that provides a wide range of tools for processing, measuring, and analyzing digital images. The software supports a variety of image file formats and is designed to work with a wide range of imaging equipment and devices.
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Vcc 600 by Physiologic Instruments
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The VCC 600 is a versatile laboratory instrument designed for a range of applications. It features a compact and robust construction, with a range of connectivity options to integrate into various experimental setups. The core function of the VCC 600 is to provide reliable and stable voltage and current control for research and testing purposes.
10
Triton x 100 by Merck Group
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Triton X-100 is a non-ionic surfactant commonly used in various laboratory applications. It functions as a detergent and solubilizing agent, facilitating the solubilization and extraction of proteins and other biomolecules from biological samples.

More about "Serous Membrane"

Serous membranes, also known as serosas or serosae, are thin, delicate layers of tissue that line various cavities within the body and cover the organs they contain.
These membranes play a crucial role in the normal functioning of the body's organ systems, such as the cardiovascular, respiratory, and reproductive systems.
They secrete a lubricating serous fluid, allowing for smooth movement and protection of the enclosed structures.
Understanding the structure and function of serous membranes is essential for medical research and clinical practice, particularly in the areas of disease diagnosis, treatment, and prevention.
Researchers often utilize various tools and techniques to study serous membranes, including Ussing chambers, FITC-dextran, Matrigel, Forskolin, Carbachol, and Image-Pro Plus 4.5 software.
Ussing chambers are widely used to measure the transport properties of serous membranes, such as transepithelial electrical resistance (TEER) and permeability.
FITC-dextran is a fluorescent tracer commonly used to assess the permeability of serous membranes.
Matrigel, a basement membrane extract, is often used to create in vitro models of serous membranes.
Forskolin and Carbachol are pharmacological agents that can be used to modulate the function of serous membranes.
Image-Pro Plus 4.5 is a powerful software tool that allows researchers to analyze and quantify various aspects of serous membrane structure and function, such as cell morphology, tight junction formation, and cytoskeletal organization.
Additionally, the VCC 600 is a specialized device used to measure the transepithelial electrical resistance of serous membranes.
Researchers may also utilize Triton X-100, a non-ionic detergent, to study the effects of membrane disruption on serous membrane properties.
By exploring the insights gained from the MeSH term description and the metadescription, researchers can leverage the power of PubCompare.ai's AI-driven platform to discover optimized protocols, compare products, and unlock the potential of serous membrane studies.