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3-nitrotyrosine

3-Nitrotyrosine is a post-translational modification of the amino acid tyrosine, where a nitro group (-NO2) is added to the 3-position of the aromatic ring.
This modification can occur due to nitrosative stress and is a marker of oxidative damage. 3-Nitrotyrosine has been linked to a variety of pathological conditions, including neurodegenerative disorders, cardiovascular disease, and cancer.
Accurate and reproducible detection and quantification of 3-nitrotyrosine levels is crucial for understanding its role in disease processes and developing targeted therapies.
PubCompare.ai's AI-driven protocol comparison tool can help researchers quickly identify the most reliable methods from the literature, preprints, and patents to power thier 3-nitrotyrosine studies with confidence and maximiize the impact of their research.

Most cited protocols related to «3-nitrotyrosine»

1
Detailed Liver Injury and Fibrosis Protocols
Alcoholic and hepatitis B-associated cirrhotic liver samples (stage 3-4 fibrosis) were collected from donor livers during liver transplantation from the Liver Tissue Cell Distribution System (LTCDS), University of Minnesota. The LTCDs were supported by NIH Contract #N01-DK-7-0004 / HHSN267200700004C. Additional information on the sample preparation, age and gender of the donors is provided in the Supplemental Materials.
The details of the induction of liver injury and fibrosis by CCl4 and bile duct ligation (BDL) and the treatment protocols are described in the Supplemental Methods.
The determination of liver function, histology and immunohistochemistry, quantitative analysis of hepatic fibrosis are described in the Supplemental Methods.
The determination of hepatic PARP and myeloperoxidase activities, 4-hydroxynonenal (4-HNE), 3-nitrotyrosine (3-NT), and hydroxyproline contents, real-time PCR, Western immunoblot analysis are described in the Supplemental Methods.
Other procedures such as isolation and treatments of murine hepatic hepatocytes and stellate cells, cell death determination by flow cytometer and activation of hepatic stellate cells are also described in the Supplemental Methods.
Mukhopadhyay P., Rajesh M., Cao Z., Horváth B., Park O., Wang H., Erdelyi K., Holovac E., Wang Y., Liaudet L., Hamdaoui N., Lafdil F., Haskó G., Szabo C., Boulares A.H., Gao B, & Pacher P. (2014). Poly (ADP-ribose) polymerase-1 is a key mediator of liver inflammation and fibrosis. Hepatology (Baltimore, Md.), 59(5), 1998-2009.
Publication 2013
3-nitrotyrosine 4-hydroxy-2-nonenal Alcoholics CCL4 protein, human Cell Death Cells Cell Transplantation Determination of Death Donors Duct, Bile Fibrosis Fibrosis, Liver Hepatic Stellate Cells Hepatitis B Hepatocyte Hydroxyproline Immunohistochemistry Injuries isolation Ligation Liver Mus Peroxidase Portal System Real-Time Polymerase Chain Reaction Tissue Donors Tissues Tissue Transplantation Transplantation Treatment Protocols Western Blot
2
Alcohol-Induced Cardiac Dysfunction

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Publication 2012
3-nitrotyrosine Apoptosis Blood Pressure Caspase 3 Cell Lines Cells Culture Media Cytokinesis dextrin maltose Diet dihydroethidium dihydrorhodamine 123 Echocardiography Enzyme Immunoassay Ethanol Fluorescent Probes Gas Scavengers Heart inhibitors Institutional Animal Care and Use Committees Males Manganese Superoxide Dismutase Mice, Inbred C57BL MnTMPyP Mus NCF1 protein, human Oxide, Nitric Peroxynitrite Plasma Porphyrins Proteins Protein Subunits Real-Time Polymerase Chain Reaction RNA, Messenger RNA, Small Interfering Superoxides Tail Tissues Translocation, Chromosomal Western Blot
3
Immunohistochemical Analysis of Inflammatory Markers
Immunohistochemical analysis was performed as previously described at 4 days after DNBS administration [31 (link)]. The sections were incubated overnight with primary antibodies: anti-ICAM-1 mouse polyclonal antibody (1:100 in PBS, v/v, Santa Cruz Biotechnology SCB, D.B.A, Milan, Italy), anti-P-selectin mouse polyclonal antibody (1:100 in PBS, v/v, SCB, D.B.A, Milan, Italy), anti-PARP mouse polyclonal antibody (1:100 in PBS, v/v, SCB, D.B.A, Milan, Italy), anti-nitrotyrosine rabbit polyclonal antibody (1:200 in PBS, v/v, Millipore, D.B.A, Milan, Italy), anti-MPO (Neomarkers, 1:200 D.B.A, Milan, Italy). All sections were washed with PBS and then treated as previously reported [31 (link)].
Five stained sections from each mouse were scored in a blinded fashion and observed using a Leica DM6 microscope (Leica Microsystems SpA, Milan, Italy) following a typical procedure [36 (link)]. The histogram profile is related to the positive pixel intensity value obtained [37 (link)].
Siracusa R., Fusco R., Peritore A.F., Cordaro M., D’Amico R., Genovese T., Gugliandolo E., Crupi R., Smeriglio A., Mandalari G., Cuzzocrea S., Di Paola R, & Impellizzeri D. (2020). The Antioxidant and Anti-Inflammatory Properties of Anacardium occidentale L. Cashew Nuts in a Mouse Model of Colitis. Nutrients, 12(3), 834.
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Publication 2020
2,4-dinitrofluorobenzene sulfonic acid 3-nitrotyrosine Antibodies Antibodies, Anti-Idiotypic Intercellular Adhesion Molecule-1 Mice, House Microscopy Rabbits SELP protein, human
4
Splenic Cell Nitrotyrosine Analysis
Spleens were harvested under sterile conditions. Single-cell suspensions were prepared, and red cells were removed using ACK lysing buffer. Two million splenocytes were incubated for 30 min on ice in staining media (1% FBS in PBS) with the relevant Abs and then washed with PBS. For intracellular staining of nitrotyrosine, cells were labeled with anti-CD11b-APC, anti-Ly6C-FITC, and anti-Ly6G-PE, fixed and permeabilized with Cytofix/Cytoperm Buffer (BD Biosciences) and washed with a 1× PermWash solution (BD Biosciences). The cells were incubated with rabbit polyclonal anti-nitrotyrosine antibody for 1 hr on ice. After washing, the cells were incubated with the secondary detection reagents, goat anti-rabbit IgG (H+L)-Alexa Fluor 647 for 45 min on ice. After washing, the samples were analyzed using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA) and were analyzed using FlowJo software (Tree Star, Ashland, OR).
Youn J.I., Nagaraj S., Collazo M, & Gabrilovich D.I. (2008). Subsets of Myeloid-Derived Suppressor Cells in Tumor Bearing Mice. Journal of immunology (Baltimore, Md. : 1950), 181(8), 5791-5802.
Publication 2008
3-nitrotyrosine Alexa Fluor 647 anti-IgG Antibodies, Anti-Idiotypic Buffers Cells Erythrocytes Fluorescein-5-isothiocyanate Goat ITGAM protein, human Protoplasm Rabbits Sterility, Reproductive Trees
5
Immunohistochemical Profiling of Tissue Samples
Immunohistochemical analysis was performed as already described [49 (link)]. The sections were incubated overnight with primary antibodies: anti-TGF-β antibody (1:250, Santa Cruz Biotechnology), anti-(α-sma antibody (1:250, Santa Cruz Biotechnology), anti-nitrotyrosine antibody (1:500, Millipore), anti-PARP antibody (1:250, Santa Cruz Biotechnology), anti-CD4 (1:250, Santa Cruz Biotechnology) and anti-CD8 (1:250, Santa Cruz Biotecnology). All sections were washed with PBS and then treated as previously reported [50 (link)]. Stained sections from each mouse were scored in a blinded fashion and observed using a Leica DM6 microscope (Leica Microsystems SpA) following a typical procedure [51 (link)]. The histogram profile is related to the positive pixel intensity value obtained [52 (link)]. The number of positive cells was counted in three sections per animal and presented as the number of positive cells per high-power field.
Fusco R., Siracusa R., D’Amico R., Cordaro M., Genovese T., Gugliandolo E., Peritore A.F., Crupi R., Di Paola R., Cuzzocrea S, & Impellizzeri D. (2020). Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Inhibitor as a Novel Therapeutic Tool for Lung Injury. International Journal of Molecular Sciences, 21(20), 7761.
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Publication 2020
3-nitrotyrosine Animals Antibodies Antibodies, Anti-Idiotypic Immunoglobulins Microscopy Mus Transforming Growth Factor beta

Most recents protocols related to «3-nitrotyrosine»

1
Multiparameter Immunohistochemical Analysis
Mouse monoclonal primary antibodies included: anti-CD66b (80H3, AbD Serotec), anti-CD163 (GHI/61), anti-3 nitrotyrosine (3-NT), and anti-smooth muscle cell actin (SMC-actin) (Santa Cruz Biotechnologies, Inc. CA), HIF-1α (GT10211, Gen Tex). Rabbit primary polyclonal antibodies included: anti-CD68 (ProteinTech, USA), anti-CD36 (SR-B3, Novus), anti- myeloperoxidase (MPO) (ab45977, Abcam, UK), anti-neutrophil elastase (NE) (Calbiochem, San Diego, CA), anti-CD163 (M-96) (Sc-33560, Santa Cruz Biotechnologies, Inc. CA) anti-CD31 (ab32457, Abcam, UK), and VEGF (Abcam, UK). Secondary antibodies included: Cy2 (CF 488A)-conjugated goat anti-rabbit IgG and/or Cy5 (CF 647)-conjugated goat anti-mouse IgG (Biotium, Hayward, CA). Isotype controls included: purified mouse IgG1(clone MG1-45) and IgG2 (clone MOPC-173, BioLegend, San Diego, CA), and rabbit IgG (sc-2027, Santa Cruz Biotechnologies, Santa Cruz, CA).
Lavie L., Si-on E, & Hoffman A. (2023). Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques – An activated phenotype. Frontiers in Immunology, 14, 1101569.
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Publication 2023
3-nitrotyrosine Actins anti-IgG Antibodies CD163 protein, human CEACAM8 protein, human Clone Cells Goat IgG1 IgG2 Immunoglobulin Isotypes Monoclonal Antibodies Mus myeloma protein MOPC 173 Myocytes, Smooth Muscle Neutrophil neutrophil elastase, human Novus Peroxidase Rabbits SERPINA1 protein, human Vascular Endothelial Growth Factors
2
Carotid Plaque Histological Analysis
Carotid plaques were removed by standard surgical techniques and minimal manipulation to the specimens. Immediately after the surgery, plaques were stored in phosphate buffered saline (PBS) at 4°C. The specimens were embedded into an optimum cutting temperature (OCT) compound (LEICA, 020108926) and stored at -80°C for further analysis. Samples were analyzed by immunohistology, immunohistochemistry, and immunofluorescence using confocal microscopy. For immunohistochemistry plaque samples were analyzed for various CD cellular markers including CD66b, CD163, CD68, and lipids. Additional cellular markers were used for confocal microscopy. Quantitative analyses of the expression of various markers were performed as previously described (2 (link), 15 (link)) and as specified below. Mouse monoclonal primary antibodies were used to identify neutrophils (anti-CD66b), macrophages-foam cells (anti-CD163) and anti-3-nitrotyrosine for oxidative-nitrosative stress. Rabbit primary polyclonal antibodies were used for double-labeling the cells with additional markers including scavenger receptors anti-CD68 and anti-CD36, anti-NE, anti-MPO, anti-Vascular Endothelial Growth Factor (VEGF), anti- CD31, and anti- smooth muscle actin (SMC-actin). Polyclonal anti-CD163 was used for double-labeling with anti-CD66b. Intra/extra cellular lipids and lipid crystals were determined by immunohistochemistry with Oil Red O staining (15 (link)).
Lavie L., Si-on E, & Hoffman A. (2023). Giant phagocytes (Gφ) and neutrophil-macrophage hybrids in human carotid atherosclerotic plaques – An activated phenotype. Frontiers in Immunology, 14, 1101569.
Full text: Click here
Publication 2023
3-nitrotyrosine Actins Antibodies Antioxidative Stress Carotid Arteries CD163 protein, human CEACAM8 protein, human Cells Dental Plaque Foam Cells Immunofluorescence Immunohistochemistry Lipids Macrophage Microscopy, Confocal Monoclonal Antibodies Mus Neutrophil Nitrosation Operative Surgical Procedures Phosphates Rabbits Saline Solution Scavenger Receptor Senile Plaques Smooth Muscles Vascular Endothelial Growth Factors
3
Quantifying Hippocampal Neurodegeneration Markers
The optical fractionator method of stereology of the stereo-investigator software
(MBF Biosciences) was used to quantify nitrotyrosine, cleaved caspase 3, and
NeuN immuno-reactive cells in the hippocampal dentate gyrus region. For all
measurements, six sections were obtained to cover the entire impact region,
−1.60 mm to −6.3 mm from Bregma, corresponding to every 12 serial sections for
each brain. For the stereological quantitation, we used a grid spacing of 75  μm
× 75 μm in the x and y-axis and guard zones of 2 μm at the top and bottom of
each section where immuno-positive cell bodies were counted. The total number of
nitrotyrosine, cleaved caspase 3, and NeuN-positive cells in the volume of
interest were automatically determined and expressed as
cells/mm3.
Image J software (NIH) was used to measure immunoblot protein band signal
intensity, the internal length, and the central width of each endothelial
nucleus.
Tchantchou F., Hsia R.C., Puche A, & Fiskum G. (2023). Hippocampal vulnerability to hyperhomocysteinemia worsens pathological outcomes of mild traumatic brain injury in rats. Journal of Central Nervous System Disease, 15, 11795735231160025.
Publication 2023
3-nitrotyrosine Brain Caspase 3 Cell Body Cells Epistropheus Gyrus, Dentate Immunoblotting Proteins Vision
4
Brain Sections Immunohistochemistry Protocol
Brain sections were co-stained for the expression of the oxidative stress
marker nitrotyrosine and the neuron-specific neuronal nuclear antigen
(NeuN), nitrotyrosine, and the astrocyte marker the glial fibrillary acidic
protein (GFAP) or the apoptotic marker cleaved caspase-3 and NeuN as
previously described.34 (link),35 (link),38 (link) Briefly,
free-floating sections were rinsed in phosphate-buffered saline (PBS) and
blocked in 1% horse serum in PBS containing .3% Triton X for 1 h. Sections
were then transferred in an antibody mixture containing rabbit
anti-nitrotyrosine polyclonal antibody (1:2500; Sigma, MO) and chicken
anti-GFAP polyclonal antibody (1:3000; Novus Biologicals, CO), rabbit
anti-nitrotyrosine polyclonal antibody (1:2500; Sigma, MO) and mouse
anti-NeuN monoclonal antibody clone, A60 (1:1500; Millipore/Sigma, MO) or
rabbit anti-cleaved caspase 3 polyclonal antibody (1:7500; Millipore, MA)
and mouse anti-NeuN monoclonal antibody clone, A60 (1:1500 Millipore/Sigma,
MO) and incubated at 4°C overnight. Sections were washed in PBS and
incubated in a mixture of corresponding secondary antibodies containing
Alexa Fluor 594 and Alexa Fluor 488 or Alexa Fluor 547 (1:2000; Invitrogen,
NY) for 1 h at room temperature. Sections were washed with PBS,
counterstained with 4,6-diamidino-2-phenylindole (DAPI), and mounted with an
anti-fade mounting medium (Vector Labs, CA).
Tchantchou F., Hsia R.C., Puche A, & Fiskum G. (2023). Hippocampal vulnerability to hyperhomocysteinemia worsens pathological outcomes of mild traumatic brain injury in rats. Journal of Central Nervous System Disease, 15, 11795735231160025.
Publication 2023
3-nitrotyrosine alexa fluor 488 Antibodies Antibodies, Anti-Idiotypic Antigens, Nuclear Apoptosis Astrocytes Biological Factors Brain Caspase Caspase 3 Clone Cells Cloning Vectors Equus caballus Immunoglobulins Monoclonal Antibodies Mus Neuroglia Neurons Novus Phosphates Saline Solution Serum
5
Biomarker Assessment in Fasting Blood
Fasting blood samples were collected from the jugular vein at baseline and monthly. Blood was collected in 0.1 mol/L citrate-containing EDTA. Fresh whole blood was submitted to Antech Diagnostics for CBC with differential and biochemistry measurements (Superchem w/CBC, SA020). Plasma IGF-1 levels were quantified by human IGF-I Quantikine ELISA Kit (R&D Systems, DG100B) and CRP levels by porcine C-reactive protein/CRP DuoSet ELISA (R&D Systems, DY2648). Quantification of plasma N-tyrosine and TAC assay were performed with OxiSelect Nitrotyrosine ELISA Kit (STA-305) and TAC assay kit (STA-360) (both from Cell Biolabs Inc).
Sukhanov S., Higashi Y., Yoshida T., Danchuk S., Alfortish M., Goodchild T., Scarborough A., Sharp T., Jenkins J.S., Garcia D., Ivey J., Tharp D.L., Schumacher J., Rozenbaum Z., Kolls J.K., Bowles D., Lefer D, & Delafontaine P. (2023). Insulin-like growth factor 1 reduces coronary atherosclerosis in pigs with familial hypercholesterolemia. JCI Insight, 8(4), e165713.
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Publication 2023
3-nitrotyrosine Biological Assay BLOOD Cells Citrates C Reactive Protein Diagnosis Edetic Acid Enzyme-Linked Immunosorbent Assay Homo sapiens IGF1 protein, human Jugular Vein Pigs Plasma Tyrosine

Top products related to «3-nitrotyrosine»

1
Nitrotyrosine by Merck Group
Sourced in United States, United Kingdom
Nitrotyrosine is a laboratory product used for the detection and quantification of nitrated proteins. It is a molecule formed by the nitration of the amino acid tyrosine, which can serve as a marker for oxidative and nitrosative stress in biological samples.
2
Anti nitrotyrosine antibody by Merck Group
Sourced in United States, Italy, Germany
The Anti-nitrotyrosine antibody is a laboratory tool used to detect and measure the presence of nitrotyrosine, a post-translational modification associated with oxidative stress and various pathological conditions. It is a specific and sensitive reagent for immunohistochemical, Western blotting, and other analytical techniques.
3
Anti nitrotyrosine by Merck Group
Sourced in United States, Germany, Italy
Anti-nitrotyrosine is a lab equipment product used for the detection and quantification of nitrotyrosine, a post-translational modification that occurs in proteins under conditions of nitrosative stress. It serves as a marker for oxidative and nitrosative damage.
4
Oxiselect nitrotyrosine elisa kit by Cell Biolabs
Sourced in United States
The OxiSelect™ Nitrotyrosine ELISA Kit is a quantitative assay designed to measure the levels of nitrotyrosine-modified proteins in biological samples. It provides a simple and reliable method for the detection and quantification of nitrotyrosine-containing proteins.
5
Nitrotyrosine by Santa Cruz Biotechnology
Sourced in United States
Nitrotyrosine is a chemical compound that is used to detect and quantify the levels of nitrotyrosine in biological samples. Nitrotyrosine is formed when tyrosine residues in proteins are modified by reactive nitrogen species, such as peroxynitrite, and is considered a marker of nitrosative stress.
6
3 nitrotyrosine elisa kit by Abcam
Sourced in United Kingdom, United States
The 3-Nitrotyrosine ELISA Kit is a quantitative assay designed to measure the levels of 3-nitrotyrosine, a post-translational modification associated with oxidative stress and inflammatory conditions. The kit utilizes an enzyme-linked immunosorbent assay (ELISA) format to detect and quantify the target analyte in biological samples.
7
β actin by Santa Cruz Biotechnology
Sourced in United States, United Kingdom, China, Germany, Canada, Morocco, Japan, Italy, Switzerland, France, Israel, Singapore, Hong Kong, Sweden, Macao, Panama
β-actin is a cytoskeletal protein that is ubiquitously expressed in eukaryotic cells. It is a component of the microfilament system and plays a crucial role in various cellular processes, such as cell motility, maintenance of cell shape, and intracellular trafficking.
8
Ab61392 by Abcam
Sourced in United Kingdom, United States
Ab61392 is a laboratory equipment product. It is a tool used for scientific research and analysis. The core function of this product is to facilitate specific tasks or processes within a laboratory setting.
9
3 nitrotyrosine 3 nt by Merck Group
Sourced in United States
3-nitrotyrosine (3-NT) is a chemical compound used as a laboratory reagent. It is a modified amino acid that serves as a marker for oxidative and nitrative stress in biological samples. 3-NT can be used in various analytical and research applications to study cellular processes and pathological conditions.
10
3 nitrotyrosine by Abcam
Sourced in United States
3-nitrotyrosine is a modified amino acid that can be used as a biomarker for oxidative stress and nitrosative damage. It is a stable end-product of the reaction between nitric oxide, superoxide, and tyrosine residues in proteins.

More about "3-nitrotyrosine"

3-Nitrotyrosine (3-NT), a post-translational modification of the amino acid tyrosine, is a key marker of oxidative and nitrosative stress.
This modification involves the addition of a nitro group (-NO2) to the 3-position of the aromatic ring of tyrosine.
Accurate quantification of 3-nitrotyrosine levels is crucial for understanding its role in a variety of pathological conditions, including neurodegenerative disorders, cardiovascular disease, and cancer.
PubCompare.ai's AI-driven protocol comparison tool can help researchers quickly identify the most reliable and reproducible methods for detecting and quantifying 3-nitrotyrosine from the scientific literature, preprints, and patents.
This ensures researchers can power their 3-nitrotyrosine studies with confidence and maximize the impact of their research.
The detection of 3-nitrotyrosine can be achieved using a variety of techniques, such as ELISA kits (e.g., OxiSelect™ Nitrotyrosine ELISA Kit, 3-Nitrotyrosine ELISA Kit) and antibody-based methods (e.g., anti-nitrotyrosine antibodies like Ab61392).
Additionally, researchers can use β-actin as a housekeeping gene to normalize 3-nitrotyrosine levels.
By leveraging the insights from PubCompare.ai's AI-powered tool, researchers can identify the most reliable and reproducible protocols to advance their understanding of the role of 3-nitrotyrosine in disease processes and develop targeted therapies.