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ABCC1 protein, human

The ABCC1 protein is a member of the ATP-binding cassette (ABC) transporter superfamily, which plays a crucial role in the transport of various molecules across cell membranes.
In humans, ABCC1, also known as the multidrug resistance-associated protein 1 (MRP1), is expressed in many tissues and is involved in the efflux of a wide range of substrates, including drugs, toxins, and signaling molecules.
This protein is particularly important in the regulation of cellular detoxification processes and can contribute to drug resistance in certain cancers.
Understanding the function and regulation of ABCC1 is of significant interest in the fields of pharmacology, toxicology, and cancer biology.
Reserach on the ABCC1 protein can provide valuable insights into its role in human health and disease, and help develop targeted therapies and improve patient outcomes.

Most cited protocols related to «ABCC1 protein, human»

Measuring ABC Transporter Efflux Activity

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Publication 2013

ABCB1 protein, human ABCC1 protein, human BI 2536 Biological Assay Cells fluorexon MK 0571 pheophorbide a tariquidar

Quantitative Assessment of miRNA and Transporters

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Publication 2011

ABCB1 protein, human ABCC1 protein, human ATP-Binding Cassette Transporters Biological Markers Cultured Cells Dehydrogenase, Aldehyde DNA, Complementary Genes Glycoproteins MicroRNAs multidrug resistance-associated protein 1 Oligonucleotide Primers Reverse Transcription RNA RNA, Messenger Small Nucleolar RNA Stem, Plant Stem Cells SYBR Green I Technique, Dilution trizol

MTT Assay for Anticancer Drug Sensitivity

The MTT colorimetric assay was used to detect the sensitivity of the cells against anticancer drugs. Cells (5 × 10³/well) were seeded evenly into (160 μl/well) 96-well plates and cultured overnight. For the reversal experiments, ORA, OSA, and parallel control modulators (20 μl/well) were added 1 h prior. Different concentrations of the chemotherapeutic drugs (20 μl/well) were then added into the designated wells. After 72 h of incubation, 20 μl of MTT solution (4 mg/ml) was added to each well, and the plate was further incubated for 4 h. Subsequently, the medium was discarded, and 100 μl of dimethylsulfoxide (DMSO) was added into each well to dissolve the formazan crystals. The absorbance was determined at 570 nm by the OPSYS microplate reader (DYNEX Technology, Inc., Chantilly, VA). The IC₅₀ value was calculated from the survival curves using the modified Bliss method [35 (link)]. Verapamil was used at a nontoxic concentration of 3 μM as a positive control for ABCB1 overexpressing cell lines. FTC at 3 μM and PAK-104P at 10 μM were used as positive control for ABCG2 and ABCC1 overexpressing cell lines, respectively.

Zhang Y.K., Zhang H., Zhang G.N., Wang Y.J., Kathawala R.J., Si R., Patel B.A., Xu J, & Chen Z.S. (2015). Semi-synthetic ocotillol analogues as selective ABCB1-mediated drug resistance reversal agents. Oncotarget, 6(27), 24277-24290.

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Publication 2015

ABCB1 protein, human ABCC1 protein, human Biological Assay Cell Lines Cells Colorimetry Formazans Hypersensitivity PAK 104P Pharmaceutical Preparations Pharmacotherapy Sulfoxide, Dimethyl Verapamil

CRISPR-Mediated Gene Disruption in Cell Lines

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Publication 2019

A549 Cells ABCC1 protein, human Cells Clone Cells Clustered Regularly Interspaced Short Palindromic Repeats Frameshift Mutation Freezing Genes Genome INDEL Mutation Lipofectamine Oligonucleotide Primers Phenotype Plasmids Transfection

Establishing HEK293-Derived Cell Lines

HEK293/pcDNA3.1, HEK/ABCB1 and HEK/ABCC1 cells were established by transfecting HEK293 with either the empty pcDNA3.1 or vector containing the full length ABCB1 (HEK/ABCB1) or ABCC1 (HEK/ABCC1), and were cultured in a medium containing 2 mg/ml of G418 [34 ]. The human colon cancer cell line SW620 and its doxorubicin-selected ABCB1-overexpressing SW620/Ad300 cell line were used for reversal study. Non-small cell lung cancer NCI-H460 cells and mitoxantrone-selected ABCG2-overexpressing NCI-H460/MX20 cells were kindly provided by Drs. Susan Bates and Robert Robey (NCI, NIH, Bethesda, MD). All the cell lines were grown as adherent monolayers in flasks with DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin in a humidified incubator containing of 5% CO₂ at 37°C.

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Publication 2015

ABCB1 protein, human ABCC1 protein, human antibiotic G 418 Cancer of Colon Cell Lines Cells Cloning Vectors Culture Media Doxorubicin Fetal Bovine Serum Homo sapiens Mitoxantrone Non-Small Cell Lung Carcinoma Penicillins Streptomycin

Most recents protocols related to «ABCC1 protein, human»

Biomarker Profiling in Depression

Venous blood samples will be collected for serum, plasma, DNA, and RNA extraction. Identifying biomarkers relevant to the course of depression is an area of research that is evolving rapidly. Thus, based on an ongoing critical literature review, the search for and analysis of specific biomarkers may change during the study period. Currently, the blood biomarkers include inflammation parameters (e.g., high sensitivity CRP) [61 (link), 62 (link)] and neurotrophic factors (e.g., BDNF and S100B) [63 , 64 (link)].
DNA from blood samples will be used for microarray-based genotyping of MDD candidate genes, genes of relevance for MDD (e.g., rs41271330, 5-HTTLPR, COMT, and BDNFval66met), drug metabolism (e.g., CYP2D6, CYP2C19, UGT1A1, ABCB1, ABCC1) and to compute polygenic risk scores in all participants after genome-wide genotyping in the future. DNA will also be used for epigenetic analysis, and circular extrachromosomal DNA, a form of decomposed free DNA [65 (link)], will be extracted and characterised. RNA will be extracted for gene transcription profiles using microarray or TAG-based methods (mRNA and microRNA).
DNA from blood samples will be used for microarray-based genotyping of MDD candidate genes, genes of relevance for MDD (e.g., rs41271330, 5-HTTLPR, COMT, and BDNFval66met), drug metabolism (e.g., CYP2D6, CYP2C19, UGT1A1, ABCB1, ABCC1) and to compute polygenic risk scores in all participants after genome-wide genotyping in the future. DNA will also be used for epigenetic analysis, and circular extrachromosomal DNA, a form of decomposed free DNA [65 (link)], will be extracted and characterised. RNA will be extracted for gene transcription profiles using microarray or TAG-based methods (mRNA and microRNA).
Gene analyses will be based on a priori models of genetic variations known to modulate pharmacotherapy and psychotherapy responses. The results will be used to calculate a polygenic risk score for diagnosis and treatment response and meta-analyses with established polygenic risk scores for MDD and those currently developed for anxiety and anxiety disorders, including treatment response [66 (link)].

Jensen K.H., Dam V.H., Ganz M., Fisher P.M., Ip C.T., Sankar A., Marstrand-Joergensen M.R., Ozenne B., Osler M., Penninx B.W., Pinborg L.H., Frokjaer V.G., Knudsen G.M, & Jørgensen M.B. (2023). Deep phenotyping towards precision psychiatry of first-episode depression — the Brain Drugs-Depression cohort. BMC Psychiatry, 23, 151.

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Publication 2023

ABCB1 protein, human ABCC1 protein, human Anxiety Anxiety Disorders Biological Markers BLOOD COMT protein, human CYP2C19 protein, human Cytochrome P-450 CYP2D6 Diagnosis DNA, A-Form DNA, Circular Genes Genetic Diversity Genome Hypersensitivity Inflammation Metabolism Microarray Analysis MicroRNAs Nerve Growth Factors Pharmaceutical Preparations Pharmacotherapy Plasma Psychotherapy RNA, Messenger Serum Transcription, Genetic UGT1A1 protein, human Veins

Colorectal Cancer Xenograft Mouse Model

The mice were repeatedly treated and weighed every 2 days, and the tumor volume was measured with a vernier caliper according to the equation: volume (mm³) = (length × width²)/2. On day 14, all mice were euthanized, and an autopsy was conducted. The tumors were detached, weighed, and fixed with 10% neutral formalin. Tumor tissues were embedded with paraffin, sectioned at a thickness of 5 μm, and mounted onto slides for histological examination. Tumor growth status and organ tissue lesions were observed using HE staining. Ki-67 assay was used to detect the proliferation of colorectal cancer cells in tumor tissues. The cleaved caspase-3 was investigated by immunohistochemical analysis according to a previously reported method [39 (link)]. The expression of MDR protein ABCB1, ABCC1, and ABCG2 were investigated by immunohistochemical analysis according to a previously reported method.

Pei M., Liu K., Qu X., Wang K., Chen Q., Zhang Y., Wang X., Wang Z., Li X., Chen F., Qin H, & Zhang Y. (2023). Enzyme-catalyzed synthesis of selenium-doped manganese phosphate for synergistic therapy of drug-resistant colorectal cancer. Journal of Nanobiotechnology, 21, 72.

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Publication 2023

ABCC1 protein, human Autopsy Biological Assay Caspase 3 Cell Proliferation Colorectal Carcinoma Formalin Mus Neoplasms P-Glycoprotein Paraffin Embedding Tissues

Quantitative Gene Expression Analysis of Drug Transporters

Total RNA was isolated using TRIzol^® (Invitrogen Life Technologies, Carlsbad, CA, USA) followed by complementary DNA (cDNA) synthesis using random hexamers (GeneWorks, Hindmarsh, SA, Australia) and Superscript^® II Reverse Transcriptase (Invitrogen Life Technologies).
A 27-gene Taqman^® transporter gene assay plate (Thermo Fisher Scientific, Waltham, MA, USA) was designed and carried out according to the manufacturer’s instructions. Quantitative PCR was performed on the QuantStudio 7 (Applied Biosciences, Waltham, MA, USA). Results were analyzed with the QuantStudio 7 instrument as previously described [41 (link)]. The raw data were normalized against endogenous control gene TATA-Box Binding Protein (TBP) (Life Technologies, Hs99999910_m1) using the delta-delta Ct (ΔΔCt) method as implemented in the HTqPCR Bioconductor package [42 (link)]. Triplicate gene expression values were averaged for each gene for each sample before subsequent analyses. Taqman^® FAM-NFQ-MGT labeled primers for SLC22A1, SLC22A2. SLC22A3, ABCB11, SLC2A3, ABCC1, SLC19A1, SLC29A3, SLC29A4, ABCC4, ABCF1, VDAC1, SLC25A13, ABCA2, ABCB4, ATP7A, ATP7B, TAP1, SLC7A8, ABCD1, SLCO3A1, SLC28A3, SLC29A1, SLC29A2, UCK1, UCK2, and CDA (Life Technologies, Hs00427552_ml, Hs01010723_ml, Hs01009568_ml, Hs00184824_m1, Hs00359840_m1, Hs00219905_m1, Hs00953342_m1, Hs00217911_m1, Hs00928283_m1, Hs00988734_m1, Hs00153703_m1, Hs01631624_ml, Hs00185185_m1, Hs00242232_m1, Hs00240956_m1, Hs00163707_m1, He00163739_m1, Hs00388675_m1, Hs00794796_m1, Hs00163610_m1, Hs00203184_m1, Hs00910439_m1, Hs01085706_m1, Hs00155426_m1, Hs01075618_m1, Hs00367072_m1, Hs0015601_m1, respectively) were used in this study.

Kutyna M.M., Loone S., Saunders V.A., White D.L., Kok C.H, & Hiwase D.K. (2023). Solute Carrier Family 29A1 Mediates In Vitro Resistance to Azacitidine in Acute Myeloid Leukemia Cell Lines. International Journal of Molecular Sciences, 24(4), 3553.

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Publication 2023

ABCB4 protein, human ABCB11 protein, human ABCC1 protein, human ABCD1 protein, human Anabolism ATP7A protein, human ATP7B protein, human Biological Assay DNA, Complementary Gene Expression Genes Membrane Transport Proteins Oligonucleotide Primers RNA-Directed DNA Polymerase SLC19A1 protein, human SLC29A1 protein, human SLC29A2 protein, human TATA-Box Binding Protein trizol VDAC1 protein, human

Gene expression analysis of multidrug resistance markers

RNA isolation of cell lines was performed using the NucleoSpin^® RNA Plus kit (Machery-Nagel (Allentown, PA, USA); 740984). RNA was converted to cDNA using superscript II reverse transcriptase (Invitrogen (Waltham, MA, USA); 1080-044). Gene expression of the resultant cDNA template was assessed by quantitative reverse transcription PCR (CFX384 RT-PCR machine; BIORAD (Hercules, CA, USA)) using iQ SYBR SuperMix (BIORAD; 1708884). Primer sequences were utilised as follows: YB-1 forward (5′ AAG AAG GTC ATC GCA ACG AAG 3′) and YB-1 reverse (5′ CTC CTA CAC TGC GAA GGT ACT 3′); ABCB1 forward (5′ CCC ATC ATT GCA ATA GCA GG 3′) and ABCB1 reverse (5′ GTT CAA ACT TCT GCT CCT GA 3′); ABCC1 forward (5′ TTC TCG GAA ACC ATC CAC GA 3′) and ABCC1 reverse (5′ CCT GTG ATC CAC CAG AAG GT 3′); GAPDH forward (5′ ATG TTC GTC ATG GGT GTG AA 3′) and GAPDH reverse (5′ CTC TTC TGG GTG GCA GTG AT 3′). The housekeeping gene GAPDH was used as a control to normalize the data and the relative mRNA expression level was calculated using ΔC_q/ΔΔC_q methodology [14 (link)].

Taylor L., Wade P.K., Johnson J.E., Aldighieri M., Morlando S., Di Leva G., Kerr I.D, & Coyle B. (2023). Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug Signature. Cancers, 15(4), 1086.

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Publication 2023

ABCB1 protein, human ABCC1 protein, human Cell Lines DNA, Complementary GAPDH protein, human Gene Expression Genes, Housekeeping isolation Oligonucleotide Primers Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription RNA, Messenger RNA-Directed DNA Polymerase

RNA Isolation and Real-Time PCR Analysis

RNA isolation was carried out by using guanidine isothiocyanate (TRIzol reagent, Invitrogen Corporation, Carlsbad, CA, USA). Cells were lysed in 1 mL of TRIzol reagent and then 200 µL of chloroform was added. The mixture was vigorously shaken, incubated at room temperature for 10–15 min and centrifuged at 12,000× g for 15 min. The aqueous phase was collected, and the RNA was precipitated by isopropanol addition. The pellet, previously washed in 75% ethanol, was dissolved in 10 mM Tris-HCl pH 7.5, 1 mM EDTA. Purified RNA samples were processed with the DNA-free DNA Removal Kit (Invitrogen, Thermo Fisher Scientific, Milan, Italy) to remove contaminating DNA. RNAs (2 µg) were reverse transcribed (RT) with or without the ImProm-II™ Reverse Transcriptase (Promega Italia, Milan, Italy) using oligo-dT primers in a standard 20 µL reaction. Then, 1 µL of this reaction mixture was used as a template in gene-specific amplifications performed on a StepOnePlus Real-Time PCR System using the StepOne software v2.3 (Thermo Fisher Scientific, Milan, Italy). The sequence of primers used in the amplification of FOXN3, CDKN1A and ACTB mRNAs have been previously described [27 (link)]. The sequence of primers used in the amplification of ABCB1, ABCC1, and ABCG2 mRNAs have been previously described [54 (link)]. PCR amplifications were performed in a 50 µL volume containing 25 µL SYBR green PCR master mix (Thermo Fisher Scientific, Milan, Italy) containing the ROX internal passive reference dye, 0.5 µM of each primer, and optimized MgCl₂ concentration between 1.5 and 3 mM. All determinations were performed in triplicate wells. Samples in which the RNA was not reverse-transcribed gave CT values comparable to those obtained in the no-template control well. Endpoint amplified products were subjected to melt-curve analysis. The relative quantity of the target transcript in the sample was calculated with respect to the reference ACTB mRNA using a comparative CT (ΔΔCT) method. The relative value was expressed as 2^−ΔΔCT.

Feriotto G., Marchetti P., Rondanin R., Tagliati F., Aguzzi S., Beninati S., Casciano F., Tabolacci C, & Mischiati C. (2023). Cytotoxicity of Isoxazole Curcumin Analogs on Chronic Myeloid Leukemia-Derived K562 Cell Lines Sensitive and Resistant to Imatinib. International Journal of Molecular Sciences, 24(3), 2356.

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Publication 2023

ABCB1 protein, human ABCC1 protein, human CDKN1A protein, human Cells Chloroform Edetic Acid Ethanol Gene Amplification guanidine isothiocyanate isolation Isopropyl Alcohol Magnesium Chloride oligo (dT) Oligonucleotide Primers Promega RNA, Messenger RNA-Directed DNA Polymerase SYBR Green I trizol Tromethamine

Top products related to «ABCC1 protein, human»

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The RNeasy Mini Kit is a laboratory equipment designed for the purification of total RNA from a variety of sample types, including animal cells, tissues, and other biological materials. The kit utilizes a silica-based membrane technology to selectively bind and isolate RNA molecules, allowing for efficient extraction and recovery of high-quality RNA.

Trizol reagent by Thermo Fisher Scientific

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Lipofectamine 2000 is a cationic lipid-based transfection reagent designed for efficient and reliable delivery of nucleic acids, such as plasmid DNA and small interfering RNA (siRNA), into a wide range of eukaryotic cell types. It facilitates the formation of complexes between the nucleic acid and the lipid components, which can then be introduced into cells to enable gene expression or gene silencing studies.

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The IScript cDNA Synthesis Kit is a reagent kit used for the reverse transcription of RNA into complementary DNA (cDNA). The kit contains all the necessary components to perform this reaction, including a reverse transcriptase enzyme, reaction buffer, and oligo(dT) primers.

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Dual luciferase reporter assay system by Promega

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The Dual-Luciferase Reporter Assay System is a laboratory tool designed to measure and compare the activity of two different luciferase reporter genes simultaneously. The system provides a quantitative method for analyzing gene expression and regulation in transfected or transduced cells.

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The CFX96 Real-Time System is a qPCR (quantitative Polymerase Chain Reaction) instrument designed for accurate and reliable gene expression analysis. It features a 96-well format and supports a range of fluorescent detection chemistries to enable precise quantification of target sequences.

What is the ABCC1 protein and what is its role in the human body?

The ABCC1 protein, also known as the multidrug resistance-associated protein 1 (MRP1), is a member of the ATP-binding cassette (ABC) transporter superfamily. It plays a crucial role in the transport of various molecules, including drugs, toxins, and signaling compounds, across cell membranes in humans. ABCC1 is expressed in many tissues and is involved in cellular detoxification processes, making it an important player in regulating the efflux of these substrates. Understanding the function and regulation of ABCC1 is vital in fields like pharmacology, toxicology, and cancer biology.

How can the ABCC1 protein contribute to drug resistance in certain cancers?

The ABCC1 protein can contribute to drug resistance in some cancers by actively transporting chemotherapeutic drugs out of cancer cells, reducing their effectiveness. This efflux mechanism can make certain cancer cells less susceptible to the cytotoxic effects of these drugs, leading to a reduction in their therapeutic efficacy. Researching the role of ABCC1 in drug resistance is an important area of study, as it could help develop more targeted therapies and improve patient outcomes.

What are some common research applications for the ABCC1 protein in humans?

The ABCC1 protein has a wide range of research applications in the fields of pharmacology, toxicology, and cancer biology. Researchers may study the expression, regulation, and substrate specificity of ABCC1 to understand its role in cellular detoxification processes and drug resistance. Additionally, investigating the interaction between ABCC1 and various compounds can provide insights into the development of new therapies that can overcome drug resistance mediated by this transporter. PubCompare.ai can assist researchers in identifying the most effective protocols and products related to ABCC1 protein research through its AI-driven analysis and comparisons.

Are there different variants or isoforms of the ABCC1 protein in humans?

Yes, there are multiple isoforms of the ABCC1 protein that can be found in humans. These isoforms may have slightly different structural or functional characteristics, which can impact their substrate specificity, cellular localization, and overall physiological role. Researchers investigating the ABCC1 protein should be aware of these variations and their potential implications for their specific research goals. PubCompare.ai can help researchers identify the most relevant isoforms and protocols for their studies, ensuring they are using the most appropriate tools and techniques.

How can PubCompare.ai assist researchers working with the ABCC1 protein?

PubCompare.ai can be a valuable tool for researchers working with the ABCC1 protein in humans. The platform's AI-driven analysis can help researchers screen protocol literature more efficiently and identify the most effective protocols related to ABCC1 protein research. By leveraging AI to pinpoint critical insights, PubCompare.ai can assist researchers in choosing the best protocols and products for their specific research goals, improving reproducibility and accuracy. This can be particularly helpful when working with the ABCC1 protein, as its diverse functions and potential isoforms require careful consideration of the most appropriate experimental approaches.

More about "ABCC1 protein, human"

The ABCC1 protein, also known as the multidrug resistance-associated protein 1 (MRP1), is a crucial member of the ATP-binding cassette (ABC) transporter superfamily in humans.
This protein plays a vital role in the transport of various molecules, including drugs, toxins, and signaling molecules, across cell membranes.
ABCC1 is expressed in numerous tissues and is particularly important in cellular detoxification processes, making it a key factor in understanding drug resistance in certain cancers.
Researchers studying the ABCC1 protein can utilize a variety of techniques and tools to uncover its function and regulation.
For example, the RNeasy Mini Kit and TRIzol reagent can be used for RNA extraction, while the StepOnePlus Real-Time PCR System and TaqMan Gene Expression Assays can be employed for gene expression analysis.
Transfection experiments with Lipofectamine 2000 or the IScript cDNA synthesis kit and PrimeScript RT reagent kit can provide insights into the regulation of ABCC1 expression.
Additionally, the Dual-Luciferase Reporter Assay System and CFX96 Real-Time System can be utilized to investigate the transcriptional regulation of the ABCC1 gene.
Understanding the ABCC1 protein and its role in human health and disease is of great significance in the fields of pharmacology, toxicology, and cancer biology.
Research in this area can lead to the development of targeted therapies and improved patient outcomes, making it a crucial area of study.