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Acetylcysteine

Acetylcysteine is a mucolytic agent and antioxidant used to treat a variety of respiratory conditions and for acetaminophen overdose.
It helps thin out and loosen mucus, making it easier to cough up.
Acetylcysteine also has antioxidant properties that can protect cells from damage.
It is commonly used to treat chronic bronchitis, COPD, and to prevent kidney damage from acetaminophen poisoning.
Reserachers can use PubCompare.ai to easily find and compare the best acetylcysteine research protocols from literature, preprints, and patents, optimizing their studies and improving reproducibility.

Most cited protocols related to «Acetylcysteine»

1
Maintaining Healthy Adult Brain Slices
Cited 143 times
The adult brain slice method we have described has been successfully implemented in a variety of experimental contexts for analysis of diverse brain regions and cell types. However, we would encourage adopters to view this method as a work in progress, and we believe there is still substantial room for systematic improvement. As a case in point, we have observed that mature adult brain slices experience high levels of oxidative stress due in large part to rapid depletion of cellular antioxidants including ascorbate and reduced glutathione (GSH). This can lead to lipid peroxidation, neuronal membrane rigidity, and tissue deterioration. There appears to be a nonuniform susceptibility to this form of oxidative damage, for example, CA1 and CA3 pyramidal neurons are particularly vulnerable, making patch clamp recording of these cells difficult in brain slices from adult and aging animals in spite of the protective recovery method.
The specific restoration of intracellular pools of neuronal GSH (e.g. supplementation with the cell-permeable GSH-ethyl ester) is highly effective at curbing deterioration and prolonging slice viability under these circumstances. Thus, we have been able to further improve the NMDG recovery method by devising strategies for stimulating de novo synthesis of glutathione during acute brain slice preparation and incubation. This is most readily accomplished by adding the inexpensive GSH precursor N-acetyl-L-cysteine (NAC, 5–12 mM) to the NMDG aCSF and HEPES holding aCSF formulas, but not the recording aCSF (seeNote 14). NAC is cell-permeable and has been shown to specifically increase neuronal glutathione levels in brain tissue (26 (link)). Within 1–2 hours of slice preparation we are able to observe notable improvements in the general appearance of neurons and in the ease of patch clamp recording, and the slices are able to be maintained in a healthy state for extended time periods.
Although these more advanced methods are not absolutely required for successful adult brain slice patch clamp recordings (as demonstrated by the specific application we have described in this chapter), we include this information in hopes of providing more options to extend the versatility of our method for particularly challenging applications. Glutathione restoration is highly effective at maintaining healthy brain slices but may not be appropriate in every experimental context, e.g. investigations focusing on oxidative stress in the aging brain. On the other hand, without implementing the NMDG protective recovery method together with glutathione restoration strategy, targeted patch clamp analysis in brain slices from very old animals is prohibitively challenging.
Ting J.T., Daigle T.L., Chen Q, & Feng G. (2014). Acute brain slice methods for adult and aging animals: application of targeted patch clampanalysis and optogenetics. Methods in molecular biology (Clifton, N.J.), 1183, 221-242.
Publication 2014
Acetylcysteine Adult Anabolism Animals Antioxidants Brain Cells Diet, Formula Esters Gastrin-Secreting Cells Glutathione HEPES Lipid Peroxidation Muscle Rigidity Neurons Oxidative Damage Oxidative Stress Permeability Protoplasm Pyramidal Cells Reduced Glutathione Susceptibility, Disease Tissue, Membrane Tissues
2
Culturing Primary Tumor Organoids
Cited 119 times

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Publication 2014
A-83-01 Acetylcysteine Cells Collagenase Culture Media Culture Media, Conditioned Digestion Dinoprostone Epidermal growth factor FGF10 protein, human Gastrins HEPES Homo sapiens matrigel Neoplasms Niacinamide noggin protein Penicillins Recombinant Proteins Repifermin Soybeans Streptomycin Tissues Trypsin Inhibitors
3
Culturing Intestinal Organoids with HISC
Cited 80 times

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Publication 2015
A-83-01 Acetylcysteine Culture Media, Conditioned Gastrin Homo sapiens Intestines MDFIC protein, human Neoplasms Niacinamide noggin protein Organoids SB 202190 Stem, Plant Stem Cells Tissues
4
Isolation and Culture of Intestinal Organoids
Cited 43 times
Described previously 2 (link), 4 (link). For culture/sorting experiments, at least 3 independent experiments were performed. For each experiment, crypts/cells were pooled from 3 intestines. For microarray, sorted cells from 10 intestines were pooled. Crypts were directly cultured as previously described (100 crypts/well on 24-well plate) 4 (link). For single-cell or doublet-cell culture, crypts were dissociated with TrypLE express (Invitrogen) including 2000 U/ml DNase (Sigma) for 30 min at 37 °C or 2 hr at room temperature. For reassociation assay from established crypt organoids, the samples were dissociated with TrypLE express for 15 min at 37 °C. Dissociated cells were passed through 20- μm cell strainer (Celltrix) and washed with PBS. Cells were stained with PE- conjugated anti-CD24 antibody (eBioscience) and APC- conjugated anti-Epcam antibody (eBioscience) for 15 min at 4 °C, and analyzed by MoFlo (DakoCytomation). Viable epithelial single-cells or doublets were gated by forward scatter, side scatter and pulse-width parameter, and negative staining for propidium iodide or 7-ADD (eBioscience). Sorted cells were collected, pelleted, and embedded in Matrigel (BD bioscience), followed by seeding on 96-well plate (30-50 singlets or doublets/10 μl Matrigel/well. Culture medium (Advanced DMEM/F12 supplemented with Penicillin/Streptomycin, 10 mM Hepes, Glutamax, 1× N2, 1× B27 (all from Invitrogen), and 1 μM N-acetylcysteine (Sigma) containing growth factors: 50 ng/ml EGF, 100 ng/ml noggin, 1 μg/ml R-spondin) were overlaid. Y-27632 (10 μM) was included for the first 2 days to avoid anoikis. Growth factors were added every other day and the entire medium was changed every 4 days. In some experiments, 100 ng/ml Wnt-3a (Millipore) was added in the culture medium. Sorted cells were manually inspected by inverted microscopy, and the numbers of viable organoids in triplicate were calculated.
Sato T., van Es J.H., Snippert H.J., Stange D.E., Vries R.G., van den Born M., Barker N., Shroyer N.F., van de Wetering M, & Clevers H. (2010). Paneth cells constitute the niche for Lgr5 stem cells in intestinal crypts. Nature, 469(7330), 415-418.
Publication 2010
Acetylcysteine Anoikis Antibodies, Anti-Idiotypic Biological Assay Cell Culture Techniques Cells CFC1 protein, human Culture Media Deoxyribonucleases Epithelial Cells Growth Factor HEPES Intestines matrigel Microarray Analysis Microscopy noggin protein Organoids Penicillins Propidium Iodide Pulse Rate Streptomycin TACSTD1 protein, human Y 27632
5
Culturing Lgr5+ Cells in 3D Matrix
Cited 42 times
Biliary ducts or sorted Lgr5-LacZ+ cells were isolated, mixed with Matrigel (BD Bioscience) and cultured as described in ref. 2 (link). Medium composition was AdDMEM/F12 (Invitrogen) supplemented with B27 and N2 (Invitrogen), N-Acetylcysteine (1.25 μM, Sigma), gastrin (10 nM, Sigma), EGF (50 ng/ml, Peprotech), 10% RSPO1 Conditioned Medium (kindly provided by Calvin Kuo), FGF10 (100 ng/ml, Peprotech), nicotinamide (10 mM, Sigma) and HGF (50 ng/ml, Peprotech). Detailed protocols are provided in Supplementary Methods.
Huch M., Dorrell C., Boj S.F., van Es J.H., van de Wetering M., Li V.S., Hamer K., Sasaki N., Finegold M.J., Haft A., Grompe M, & Clevers H. (2013). In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature, 494(7436), 247-250.
Publication 2013
Acetylcysteine Cells Culture Media, Conditioned Duct, Bile FGF10 protein, human Gastrin LacZ Genes matrigel Niacinamide

Most recents protocols related to «Acetylcysteine»

1
Montmorillonite Synthesis and Cytotoxicity Evaluation
Commercially available Na-montmorillonite was used (purity 97%; Zhejiang Sanding Group Co. Ltd., China). Sulfuric acid and other chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd. All reagents used in this preparation of Mts are of analytical grade purity and were used without any further purification. Dulbecco’s modified eagle medium (DMEM), penicillin/streptomycin, and fetal bovine serum (FBS) were obtained from Life Technologies (Carlsbad, CA, USA). The 2′,7′-dichlorofluorescin diacetate (H2DCF-DA), N-acetylcysteine (NAC), p38 MAPK inhibitor, extracellular signal-regulated kinases (ERK) inhibitor and c-Jun N-terminal kinases (JNK) inhibitor were bought from Sigma-Aldrich (St. Louis, MO, USA). Dihydroethidium (DHE) came from the Beyotime Biotechnology Co., Ltd. (Shanghai, China), while fluorescein sodium was obtained from Macklin Biochemical Co., Ltd (Shanghai, China).
Liu J., Yang S., Zhao L., Jiang F., Sun J., Peng S., Zhao R., Huang Y., Fu X., Luo R., Jiang Y., Li Z., Wang N., Fang T, & Zhang Z. (2023). ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo. Particle and Fibre Toxicology, 20, 8.
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Publication 2023
Acetylcysteine dichlorofluorescin dihydroethidium Eagle Extracellular Signal Regulated Kinases Fetal Bovine Serum JNK Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinase p38 Montmorrillonite Penicillins Sodium Fluorescein Streptomycin Sulfuric Acids
2
Evaluating PDTO Responses to Diverse Treatments
The evaluation of the response to treatments will be performed when PDTO have reached a diameter of 150 μm in « PDTO treatment medium », corresponding to the PDTO culture medium lacking N-Acetylcysteine, Y-27632 and primocin.
PDTO will be collected, resuspended in 2% extracellular matrix/PDTO culture medium and then platted in white and clear bottom 96-well plates previously coated with a 1:1 volume mix of PDTO treatment medium with extracellular matrix. In the case of evaluation of the response to radiotherapy, PDTO will be before irradiated using the CellRad System (FAXITRON Bioptics). In the case of evaluation of the response to chemotherapy or PARP inhibitors, drugs are prepared in 2% extracellular matrix/PDTO culture medium and added 1 hour after PDTO have been plated.
In the case of evaluation of the response to immunotherapies, PDTO will be co-cultured with PDTO specific T cells previously generated (see co-culture of PDTO with immune cells) at a 5:1 ratio. Treatments (such as Nivolumab or Pembrolizumab) will be added directly in the co-culture. A condition containing an MHC-I blocking antibody will be added to control for antigen specific killing.
PDTO morphology will be monitored by taking images during the required time using Incucyte S3 (Sartorius). At the endpoint, PDTO response will be assessed using CellTiter-Glo 3D cell viability assay (Promega) according to the manufacturer’s instruction and luminescence will be measured using GloMax Discover GM3000 (Promega) with the associated software. Results will be normalized to the control condition. IC50 will be calculated with GraphPad software. The ability of T cells to recognize and induce lysis of PDTO will be monitored via analysis of caspase 3 cleavage within PDTO and visualization of LAMP-1 on the membrane of CD8+ T cells.
The treatment response of the PDTO will be finally compared to the clinical response (PFS/DFS/OS) of the patient from whom they are derived in order to validate the predictive value of this model for HNSCC.
Perréard M., Florent R., Divoux J., Grellard J.M., Lequesne J., Briand M., Clarisse B., Rousseau N., Lebreton E., Dubois B., Harter V., Lasne-Cardon A., Drouet J., Johnson A., Le Page A.L., Bazille C., Jeanne C., Figeac M., Goardon N., Vaur D., Micault E., Humbert M., Thariat J., Babin E., Poulain L., Weiswald L.B, & Bastit V. (2023). ORGAVADS: establishment of tumor organoids from head and neck squamous cell carcinoma to assess their response to innovative therapies. BMC Cancer, 23, 223.
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Publication 2023
Acetylcysteine Antibodies, Blocking Antigens Biological Assay Caspase 3 CD8-Positive T-Lymphocytes Cells Cell Survival Coculture Techniques Cultured Cells Culture Media Cytokinesis Extracellular Matrix Immunotherapy Luminescence lysosomal-associated membrane protein 1, human Nivolumab Patients pembrolizumab Pharmaceutical Preparations Pharmacotherapy Poly(ADP-ribose) Polymerase Inhibitors Promega Squamous Cell Carcinoma of the Head and Neck T-Lymphocyte Tissue, Membrane Y 27632
3
Isolation and RNA-Seq of Mouse Intestinal Paneth Cells
Crypts were isolated from the small intestine of mice fed with SD or CDAHFD for 3 wk as previously described17 (link). Briefly, the ileum segments were shaken in cold HBSS containing 30 mM EDTA. After vigorous shaking for ~ 300 times in HBSS, the isolated crypts were resuspended in HBSS containing 300 U/mL collagenase (Sigma), 10 µM Y-27632 (Sigma), and 1 mM N-acetylcysteine (Sigma), and shaken at 180 rpm for 5 min at 37 °C on a horizontal shaker (TAITEC). Then, 50 μg/μL Dnase I (Roche) was added, and the sample was mixed by pipetting. Cells were pelleted at 500 g for 5 min at 4 °C and resuspended in washing buffer (DMEM/F12 containing 10 μM Y-27632 and 1 mM N-acetylcysteine), then passed through 40-μm cell strainer (BD Falcon). Paneth cells were stained with Zinpyr-1 (Santa Cruz) and Allophycocyanin (APC) anti-CD24 (clone M1/69,)Abcam) in washing buffer for 10 min at 37 °C. After Paneth cell labeling, the cells were sorted by flow cytometry using a cell sorter (JSAN, Bay Bioscience). Single cells were gated by forward scatter and side scatter. Cells were sorted directly into lysis buffer for RNA isolation (PureLink RNA Mini Kit, Invitrogen). To make a pooled sample, at least 10,000 Paneth cells were sorted from 2 separate mice, and sorting experiment was repeated three times. 2 pools (each pool contains Paneth cells from 6 separate mice) were prepared for each group. Total RNA was isolated using Invitrogen® PureLink RNA Purification System according to manufacturer’s instructions, and cDNA was synthesized using a SMART-Seq. Sequencing libraries were built with the TruSeq RNA Library Prep Kit (Illumina) and then submitted to Illumina NovaSeq 6000 for 100-bp PE reads sequencing. Fragments per kilobase of transcript per million mapped reads (FPKM) values were used, genes with FPKM values below 1 were not included in the analysis, and fold change ≥ 1.5 or ≤ 0.67 was considered differentially expressed.
Nakamura S., Nakamura K., Yokoi Y., Shimizu Y., Ohira S., Hagiwara M., Song Z., Gan L., Aizawa T., Hashimoto D., Teshima T., Ouellette A.J, & Ayabe T. (2023). Decreased Paneth cell α-defensins promote fibrosis in a choline-deficient L-amino acid-defined high-fat diet-induced mouse model of nonalcoholic steatohepatitis via disrupting intestinal microbiota. Scientific Reports, 13, 3953.
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Publication 2023
Acetylcysteine allophycocyanin Buffers cDNA Library Cells CFC1 protein, human Clone Cells Cold Temperature Collagenase Deoxyribonuclease I DNA, Complementary Edetic Acid Flow Cytometry Genes Hemoglobin, Sickle Ileum Intestines, Small isolation Mus Paneth Cells Y 27632 zinpyr-1
4
Synthesis and Characterization of PI-103 Compound
RIDR-PI-103 was synthesized as described [29 (link)]. PI-103 (Cat# S1038) was obtained from Selleckchem (Houston, Texas, USA). All drugs were dissolved in dimethyl sulfoxide (DMSO) (Fisher Scientific, Waltham, Massachusetts, USA) at desired concentrations and stored at −20°C. Glutathione was obtained from Fisher Scientific (Cat# BP25211). N-acetyl cysteine (NAC) was obtained from Fisher Scientific (Cat# AAA1540936). TBHP was obtained from Fisher Scientific (Cat# AC180340050). Dabrafenib (Cat# D-5699) and trametinib (Cat# T-8123) were purchased from LC Laboratories (Woburn, Massachusetts, USA).
Patel H., Mishra R., Wier A., Mokhtarpour N., Merino E.J, & Garrett J.T. (2023). RIDR-PI-103, ROS-activated prodrug PI3K inhibitor inhibits cell growth and impairs the PI3K/Akt pathway in BRAF and MEK inhibitor-resistant BRAF-mutant melanoma cells. Anti-Cancer Drugs, 34(4), 519-531.
Publication 2023
Acetylcysteine dabrafenib Glutathione Pharmaceutical Preparations PI103 Sulfoxide, Dimethyl trametinib
5
Hypertonic Stress Response in H9c2 Cells
H9c2, a rat ventricular myoblast cell line (Shanghai Cell Bank, Chinese Academy of Science) was maintained at 37˚C and 5% CO2 in Dulbecco's modified Eagle's medium (HyClone; Cytiva) supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% glutamine. H9c2 cells were seeded onto six-well plates and incubated with culture media containing NaCl (50 mM) or mannitol (100 mM), which yielded hypertonic state, equaling 400 mOsm/l, and treated with or without ROS scavenger N-acetylcysteine (NAC; 0.5 and 2 mM). Normal culture medium (isotonic, 300 mOsm/l) served as the control for the experiment. After exposure for desired time periods, the monolayer cultures were washed with D-Hanks and the cells were removed by 0.25% trypsin.
Bi Y., Yang G.H., Guo Z.Z., Cai W., Chen S.B., Zhou X, & Li Y.M. (2023). Chronic high‑salt intake induces cardiomyocyte autophagic vacuolization during left ventricular maladaptive remodeling in spontaneously hypertensive rats. Experimental and Therapeutic Medicine, 25(4), 148.
Publication 2023
Acetylcysteine Cell Lines Cells Chinese Culture Media Gas Scavengers Glutamine Heart Ventricle Mannitol Myoblasts Sodium Chloride Trypsin

Top products related to «Acetylcysteine»

1
N acetylcysteine by Merck Group
Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, Switzerland, Macao, Italy, China, France, Japan, Spain, Canada, Sweden
N-acetylcysteine is a chemical compound that is commonly used in laboratory settings. It is a precursor to the antioxidant glutathione and has various applications in research and analysis.
2
N acetyl l cysteine by Merck Group
Sourced in United States, Germany, Sao Tome and Principe, Italy, United Kingdom, Canada, Macao, Japan, China, Hungary, France
N-acetyl-L-cysteine is a chemical compound used as a lab equipment product. It is a derivative of the amino acid cysteine and has antioxidant properties.
3
N acetylcysteine nac by Merck Group
Sourced in United States, Germany, China, Italy, United Kingdom, Sao Tome and Principe, Japan, Macao, Spain, Canada, Morocco
N-acetylcysteine (NAC) is a versatile laboratory reagent commonly used in various scientific applications. It is a chemical compound that serves as a precursor to the antioxidant glutathione, playing a crucial role in cellular processes. NAC is widely utilized in biochemical research, analytical chemistry, and pharmaceutical development due to its ability to modulate redox reactions and protect against oxidative stress.
4
N acetyl l cysteine nac by Merck Group
Sourced in United States, Germany, China, Macao, Sao Tome and Principe, Canada, Japan, Italy, United Kingdom, France
N-acetyl-L-cysteine (NAC) is a chemical compound that serves as a precursor to the amino acid cysteine. It is a white, crystalline powder that is soluble in water and used in various laboratory applications.
5
Fetal bovine serum (fbs) by Thermo Fisher Scientific
Sourced in United States, China, United Kingdom, Germany, Australia, Japan, Canada, Italy, France, Switzerland, New Zealand, Brazil, Belgium, India, Spain, Israel, Austria, Poland, Ireland, Sweden, Macao, Netherlands, Denmark, Cameroon, Singapore, Portugal, Argentina, Holy See (Vatican City State), Morocco, Uruguay, Mexico, Thailand, Sao Tome and Principe, Hungary, Panama, Hong Kong, Norway, United Arab Emirates, Czechia, Russian Federation, Chile, Moldova, Republic of, Gabon, Palestine, State of, Saudi Arabia, Senegal
Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.
6
Penicillin streptomycin by Thermo Fisher Scientific
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Penicillin/streptomycin is a commonly used antibiotic solution for cell culture applications. It contains a combination of penicillin and streptomycin, which are broad-spectrum antibiotics that inhibit the growth of both Gram-positive and Gram-negative bacteria.
7
Glutamax by Thermo Fisher Scientific
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GlutaMAX is a chemically defined, L-glutamine substitute for cell culture media. It is a stable source of L-glutamine that does not degrade over time like L-glutamine. GlutaMAX helps maintain consistent cell growth and performance in cell culture applications.
8
Dimethyl sulfoxide (dmso) by Merck Group
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DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.
9
Epidermal growth factor (egf) by Thermo Fisher Scientific
Sourced in United States, United Kingdom, Germany, China, Canada, France, Italy, Japan, Israel, Switzerland, Australia, Macao, Belgium, Spain, Denmark, Jersey
EGF is a lab equipment product from Thermo Fisher Scientific. It is a recombinant human Epidermal Growth Factor (EGF) protein. EGF is a growth factor that plays a role in cell proliferation and differentiation.
10
Nicotinamide by Merck Group
Sourced in United States, Germany, United Kingdom, Japan, Switzerland, France, Sao Tome and Principe, Israel, India, Canada, Belgium, Sweden, China
Nicotinamide is a form of vitamin B3 that serves as a precursor for the coenzyme nicotinamide adenine dinucleotide (NAD) in biological systems. NAD is essential for various metabolic processes within cells, including energy production and DNA repair.

More about "Acetylcysteine"

Acetylcysteine, also known as N-acetylcysteine (NAC), is a versatile compound with a wide range of applications in the medical and scientific fields.
As a mucolytic agent and antioxidant, it is commonly used to treat various respiratory conditions, such as chronic bronchitis and COPD (Chronic Obstructive Pulmonary Disease).
Acetylcysteine helps thin and loosen mucus, making it easier for patients to cough up and clear their airways.
In addition to its respiratory benefits, acetylcysteine is also used to prevent kidney damage from acetaminophen (paracetamol) overdose.
Its antioxidant properties can protect cells from damage, making it a valuable tool in the management of acetaminophen toxicity.
Researchers often utilize acetylcysteine in their studies, either as a standalone treatment or in combination with other compounds, such as FBS (Fetal Bovine Serum), Penicillin/Streptomycin, GlutaMAX, DMSO (Dimethyl Sulfoxide), EGF (Epidermal Growth Factor), and Nicotinamide.
These additives can help maintain cell health and optimize experimental conditions.
When conducting acetylcysteine-related research, scientists can benefit from using tools like PubCompare.ai.
This AI-driven protocol comparison platform allows researchers to easily locate and compare the best protocols from literature, preprints, and patents, helping to optimize their studies and improve reproducibility.
By leveraging the insights and resources provided by PubCompare.ai, researchers can enhance the efficacy and reliability of their acetylcysteine-focused experiments.