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Annexin A1

Annexin A1 is a calcium-dependent phospholipid-binding protein that plays a key role in regulating inflammation and cell signaling.
This membran-assocaited protein is involved in a variety of cellular processes, including exocytosis, endocytosis, and apoptosis.
Annexin A1 has emerged as a promising therapeutic target for managing inflammatory disorders, cancer, and other diseases.
Researchers can leverage PubCompare.ai's AI-driven platform to identify the most reproducible and accurate experimental protocols for studying Annexin A1, enabling them to elevate their research and accelerate scientific discoveries.

Most cited protocols related to «Annexin A1»

Apoptosis and Mitochondrial Potential Evaluation

Cited 7 times

Flow cytometric analyzed of apoptosis were used the FITC Annexin V Apoptosis Detection Kit I (BD, USA), and was presented as protocol described. Briefly, cells were dissociated with trypsin and resuspended at 1 × 10⁶ cells/mL in binding buffer with 50 μg/mL FITC Annexin V and 50 μg/mL PI. The cells were subsequently incubated for 15 minutes at room temperature, and then were analyzed by Gallios flow cytometer (Beckman Coulter, USA). The cell's inner mitochondrial membrane potential (Δψm) was detected by flow cytometric using MitoScreen JC-1 staining kit (BD), and was presented as protocol described. Briefly, cells were dissociated with trypsin and resuspended at 1 × 10⁶ cells/mL in Assay Buffer, and then incubated at 37°C for 15 minutes with 10 μg/mL JC-1. Before analyzed by flow cytometer, cells were washed twice by Assay Buffer. Flow cytometry data were analyzed using FlowJo 7.6 software (TreeStar Inc., USA).

Ren D., Lin B., Zhang X., Peng Y., Ye Z., Ma Y., Liang Y., Cao L., Li X., Li R., Sun L., Liu Q., Wu J., Zhou K, & Zeng J. (2017). Maintenance of cancer stemness by miR-196b-5p contributes to chemoresistance of colorectal cancer cells via activating STAT3 signaling pathway. Oncotarget, 8(30), 49807-49823.

Publication 2017

Annexin A1 Annexin A5 Apoptosis Biological Assay Buffers Cells Flow Cytometry Fluorescein-5-isothiocyanate Membrane Potential, Mitochondrial Trypsin

Colonic Gene Expression Analysis in Mice

Cited 5 times

Mice colons were dissected at 5^th and 15^th day of the experimental period. After wash with PBS to remove the presence of feces, colons were immediately frozen in liquid nitrogen. Total RNA was extracted using TRIzol Reagent (Bio-Rad Laboratories) and following a specific RNA extraction kit (NucleoSpin®, MACHEREY-NAGEL GmbH & Co, Düren, Germany), according to the manufacturer’s instructions. cDna was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) from 4 µg total RNA. The applied PCR settings were previously described^{45 (link)}. The used gene primers were: Il1b (for IL-1β), Il6 (for IL-6), Tnfa (for TNF-α), Prostaglandin-endoperoxide synthase (Ptgs) 2 (for COX-2), Ifng (for IFN-γ), AnxA1 (for Annexin A1), Il10 (for IL-10), Tff3 (for TFF3), Muc2 (for mucin 2), Ocln (for Occludin), Tlr2 (for TLR2), MyD88 (for MYD88), Hdac9 (for HDAC9), Slc16a1 (for MCT1) and Nlrp3 (for NALP3) (Qiagen, Hilden, Germany) in a final volume of 25 μl. All studied mRNAs were normalized to Gapdh (for GAPDH) or Actb (for β-actin) as housekeeping gene, and data were analyzed according to the 2^−ΔΔCT method.

Lama A., Annunziata C., Coretti L., Pirozzi C., Di Guida F., Nitrato Izzo A., Cristiano C., Mollica M.P., Chiariotti L., Pelagalli A., Lembo F., Meli R, & Mattace Raso G. (2019). N-(1-carbamoyl-2-phenylethyl) butyramide reduces antibiotic-induced intestinal injury, innate immune activation and modulates microbiota composition. Scientific Reports, 9, 4832.

Publication 2019

Actins Annexin A1 Colon DNA, Complementary Feces Freezing GAPDH protein, human Genes Genes, Housekeeping HDAC9 protein, human IL1B protein, human IL10 protein, human Interferon Type II Interleukin-1 beta MCTS1 protein, human MUC2 protein, human Mucin-2 Mus Nitrogen Occludin Oligonucleotide Primers PTGS2 protein, human Reverse Transcription RNA, Messenger RNA Interference TLR2 protein, human trizol Tumor Necrosis Factor-alpha

Cell Cycle and Apoptosis Analysis

Cited 4 times

For cell cycle analysis, cells were collected and fixed using 70% ethanol. After washing with PBS and subsequently washing with stain buffer, 1 × 10⁶ cells were resuspended in 0.5 mL of PI/RNase Staining Buffer (BD biosciences, San Jose, CA, USA), and cells were incubated for 15 min at room temperature (RT), protected from light. A FACSCanto II flow cytometer (BD biosciences) was used to analyze cell cycle distribution. For apoptosis assay, the PE Annexin V Apoptosis Detection Kit I (BD biosciences) was used following the manufacturer’s protocols. Cells were harvested and washed with pre-cold PBS buffer twice. Then, 5 μl of PE Annexin V and 5 μl of 7-AAD solution were added to each sample, and cells were incubated for 15 min at RT. FACSCanto II flow cytometer was used to measure the cell apoptosis.

Wang Y., Yang L., Chen T., Liu X., Guo Y., Zhu Q., Tong X., Yang W., Xu Q., Huang D, & Tu K. (2019). A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis. Molecular Cancer, 18, 28.

Publication 2019

angiogenin Annexin A1 Annexin A5 Apoptosis Biological Assay Buffers Cell Cycle Cells Cold Temperature Endoribonucleases Ethanol Light Stains

Annexin V/Propidium Iodide Apoptosis Detection

Cited 4 times

For annexin V/propidium iodide (PI) staining the FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen, San Diego, CA) was used according to the manufacturer's instructions. Staining for PI was performed as previously described [51 (link)]. The data were analysed with the FlowJo software (version 8.7.1; Tree Star, Ashland, OR, U.S.A.).

Karpel-Massler G., Ramani D., Shu C., Halatsch M.E., Westhoff M.A., Bruce J.N., Canoll P, & Siegelin M.D. (2016). Metabolic reprogramming of glioblastoma cells by L-asparaginase sensitizes for apoptosis in vitro and in vivo. Oncotarget, 7(23), 33512-33528.

Publication 2016

Annexin A1 Annexin A5 Apoptosis Fluorescein-5-isothiocyanate Propidium Iodide Trees

Isolation and Characterization of Brainstem Progenitors

Cited 4 times

Normal brainstem was isolated from Ntv-a or Ntva;p53^fl/fl postnatal day 3 (P3) pups and was digested and dissociated as described above for Olig2-eGFP-L10a tumors. Cells were cultured in DMEM supplemented with 10% FBS, 2 mM L-glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin at 37°C and 5% CO₂, and passaged a maximum of 3 times for experiments. Cells were plated in clear 6-well plates for Annexin V assays, clear 96-well plates for BrdU assays, and white-walled, clear-bottomed 96-well plates for Caspase 3/7 assays.
To concentrate RCAS viruses, DF1 cells transfected with RCAS plasmids were passaged a minimum of 6 times from transfection, and then passaged 1:12. After 3 days, virus-containing media was harvested, centrifuged to remove cell debris, filtered through 0.45 μm pores, and concentrated 100-fold using Retro-X Concentrator (Clontech) per the manufacturer’s instructions.
Brainstem progenitors were plated and infected with RCAS viruses at 1:100. Assays were conducted 3–5 days post-infection, or were split when confluent and subsequently plated for assays. For BrdU assays, the Cell Proliferation ELISA, BrdU Colorimetric kit (Roche) was utilized, per the manufacturer’s instructions using a Molecular Devices Versa Max Tunable Microplate reader. Caspase 3/7 activity was measured using the ApoToxGlo Triplex assay (Promega) per the manufacturer’s instructions using a Turner Biosystems Modulus Microplate Reader. To measure the percentage of cells staining positive for Annexin V, the FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen) was utilized per the manufacturer’s instructions. For cell counting experiments, 50,000 infected cells were plated in 6 well plates in duplicate. For each time point, 2 wells of each line were trypsinized and counted with a Sceptor 2.0 Cell Counter (Millipore). All experiments were done a minimum of three times on at least three independent preparations of progenitor cells.

Misuraca K.L., Barton K.L., Chung A., Diaz A.K., Conway S.J., Corcoran D.L., Baker S.J, & Becher O.J. (2014). Pax3 expression enhances PDGF-B-induced brainstem gliomagenesis and characterizes a subset of brainstem glioma. Acta Neuropathologica Communications, 2, 134.

Publication 2014

Annexin A1 Annexin A5 Apoptosis Biological Assay Brain Stem Bromodeoxyuridine Caspase-7 Cell Proliferation Cells Colorimetry Enzyme-Linked Immunosorbent Assay Fluorescein-5-isothiocyanate Glutamine Infection Medical Devices Neoplasms OLIG2 protein, human Penicillins Plasmids Promega Stem Cells Streptomycin Transfection Virus

Most recents protocols related to «Annexin A1»

Apoptosis Evaluation by Flow Cytometry

Apoptosis was evaluated by flow cytometry (BD FACSCanto) using the FITC Annexin V Apoptosis Detection Kit I (BD Pharmingen™), following the instructions provided by the manufacturer. Briefly, differentiated CAD cells (48 h) were treated with the ACM media or Etoposide 10 µM for 24 h [50 (link)], and then harvested by trypsinization for 1 min at 37 °C. Following two rounds of washes with PBS, cells were resuspended in the binding buffer and incubated with PI and Annexin V-Alexa Fluor 488 for 30 min. Data were processed using the FlowJo v10.8.0 Software [51 (link)].

Palacios E., Lobos-González L., Guerrero S., Kogan M.J., Shao B., Heinecke J.W., Quest A.F., Leyton L, & Valenzuela-Valderrama M. (2023). Helicobacter pylori outer membrane vesicles induce astrocyte reactivity through nuclear factor-κappa B activation and cause neuronal damage in vivo in a murine model. Journal of Neuroinflammation, 20, 66.

Publication 2023

alexa fluor 488 Annexin A1 Annexin A5 Apoptosis Buffers Cells Etoposide Flow Cytometry Fluorescein-5-isothiocyanate

Quantifying Apoptosis in Burkitt's Lymphoma

Externalization of phosphatidylserine, an early marker of apoptosis, was monitored by Annexin V-PE and 7AAD staining to measure cell death (PE Annexin V Apoptosis Detection Kit I, 559763; BD Biosciences). BL cell lines treated with rituximab, in vitro, and BL tumor cells harvested from NSG-BL tumors treated with rituximab and PBS were first stained with human antibodies to CD20, CD19, and CD45, followed by Annexin V/7AAD staining. Briefly, the cells were first washed with ice-cold PBS and resuspended in 1X Annexin V binding buffer at 1 × 10⁶ cells/ml. 100 μl of this cell suspension (1 × 10⁵ cells) was then incubated with 2.5 µl of Annexin V-PE and 7AAD each, in the dark for 20 min. After incubation, cells were added to 400 µl of 1X Annexin V binding buffer and analyzed by flow cytometry. Isotype IgG antibody was tested as a negative control even though there was no difference between the media-only condition and IgG isotype.

Saikumar Lakshmi P., Oduor C.I., Forconi C.S., M’Bana V., Bly C., Gerstein R.M., Otieno J.A., Ong’echa J.M., Münz C., Luftig M.A., Brehm M.A., Bailey J.A, & Moormann A.M. (2023). Endemic Burkitt lymphoma avatar mouse models for exploring inter-patient tumor variation and testing targeted therapies. Life Science Alliance, 6(5), e202101355.

Publication 2023

7-aminoactinomycin D Annexin A1 Annexin A5 Antibodies ANXA2 protein, human Apoptosis Buffers Cell Death Cell Lines Cells Common Cold Culture Media, Conditioned Flow Cytometry Homo sapiens Immunoglobulin G Immunoglobulin Isotypes Neoplasms Phosphatidylserines Rituximab

Quantifying Cell Death via Flow Cytometry

Cell death rate was determined by flow cytometry with the FITC Annexin V Apoptosis Detection Kit I (BD). The assay was performed as follows: 2x10⁵ cells were seeded in a 6 well plate and cultured for 12 hours. Then the cells were treated with indicated concentration of H₂O₂, TRAIL for 24 hours or PAC-1 for 8 hours to induce cell death. All the cells, including those in supernatant, were harvested for further analysis. The cells were washed with cold PBS and resuspended in 100μl binding buffer. The solutions were transferred to a 5ml round bottom tube (Falcon) and the cells were stained with 5μl of FITC Annexin V and 5μl PI. The system was gently mixed and incubated for 15 min at room temperature in the dark. Before running on the flow cytometry, 200μl of PBS was added to each tube. The analysis on flow cytometry was finished within 1 hour.

Zhang F.F., Jiang C., Jiang D.P., Cui Y.Z., Wang X.Y., Sun L.Z., Chen M., Lam K.O., Wu S.Y., Verhoeft K., Kwong D.L, & Guan X.Y. (2023). ARHGAP15 promotes metastatic colonization in gastric cancer by suppressing RAC1-ROS pathway. PLOS Genetics, 19(2), e1010640.

Publication 2023

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Annexin V and PI Apoptosis Assay

Apoptosis rate was evaluated by Annexin V and PI co‐staining using FITC Annexin V Apoptosis Detection Kit I (BD Biosciences) according to the manufacturer's instructions. Briefly, 1 × 10⁵ cells were resuspended in 100 µL of 1X binding buffer and stained with 5 µL of FITC Annexin V and 5 µL PI for 15 min at room temperature. Apoptotic rates were analyzed by FACS Canto II analyzer.

Wang S., Chen J., Jiang Y., Lei Z., Ruan Y.C., Pan Y., Yam J.W., Wong M.P, & Xiao Z. (2023). Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors. Advanced Science, 10(7), 2205262.

Publication 2023

Annexin A1 Annexin A5 Apoptosis Buffers Cells FITC-annexin A5 Fluorescein-5-isothiocyanate

Quantifying Cell Apoptosis with Annexin V-FITC

The dead cells were quantitated using the FITC Annexin V Apoptosis Detection Kit I (BD Biosciences, 556 547) according to the manufacturer's instructions as described previously.^[⁵¹^] In brief, cells in the binding buffer were incubated with Annexin V/propidium iodide (PI) for 15 min before analysis using a flow cytometer (FACSCanto II; BD Biosciences).

La T., Chen S., Zhao X.H., Zhou S., Xu R., Teng L., Zhang Y.Y., Ye K., Xu L., Guo T., Jamaluddin M.F., Feng Y.C., Tang H.J., Wang Y., Xu Q., Gu Y., Cao H., Liu T., Thorne R.F., Shao F., Zhang X.D, & Jin L. (2023). LncRNA LIMp27 Regulates the DNA Damage Response through p27 in p53‐Defective Cancer Cells. Advanced Science, 10(7), 2204599.

Publication 2023

Annexin A1 Annexin A5 Apoptosis Buffers Cells Fluorescein-5-isothiocyanate Propidium Iodide

Top products related to «Annexin A1»

Fitc annexin 5 apoptosis detection kit 1 by BD

Sourced in United States, Germany, China, United Kingdom, France, Portugal, Canada, Spain, Japan, Belgium

The FITC Annexin V Apoptosis Detection Kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes the binding properties of the protein Annexin V, which has a high affinity for the phospholipid phosphatidylserine, which becomes exposed on the cell surface during apoptosis. The Annexin V is conjugated with the fluorescent dye FITC, allowing for the detection of apoptotic cells through fluorescence microscopy or flow cytometry.

Pe annexin 5 apoptosis detection kit 1 by BD

Sourced in United States, France, Belgium, Germany, China, United Kingdom, Japan

The PE Annexin V Apoptosis Detection Kit is a laboratory equipment product designed for the detection of apoptosis. It utilizes the binding properties of Annexin V, a calcium-dependent phospholipid-binding protein, which has a high affinity for phosphatidylserine (PS). The kit provides a method to identify apoptotic cells by flow cytometry.

Facscalibur by BD

Sourced in United States, Germany, United Kingdom, China, Canada, Japan, Italy, France, Belgium, Switzerland, Singapore, Uruguay, Australia, Spain, Poland, India, Austria, Denmark, Netherlands, Jersey, Finland, Sweden

The FACSCalibur is a flow cytometry system designed for multi-parameter analysis of cells and other particles. It features a blue (488 nm) and a red (635 nm) laser for excitation of fluorescent dyes. The instrument is capable of detecting forward scatter, side scatter, and up to four fluorescent parameters simultaneously.

Annexin 5 apoptosis detection kit 1 by BD

Sourced in United States

The Annexin V Apoptosis Detection Kit I is a laboratory product designed to detect and measure apoptosis, a form of programmed cell death. The kit provides the necessary reagents to label and quantify apoptotic cells using flow cytometry or fluorescence microscopy techniques.

Facscalibur flow cytometer by BD

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The FACSCalibur flow cytometer is a compact and versatile instrument designed for multiparameter analysis of cells and particles. It employs laser-based technology to rapidly measure and analyze the physical and fluorescent characteristics of cells or other particles as they flow in a fluid stream. The FACSCalibur can detect and quantify a wide range of cellular properties, making it a valuable tool for various applications in biology, immunology, and clinical research.

Facscanto 2 by BD

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The FACSCanto II is a flow cytometer instrument designed for multi-parameter analysis of single cells. It features a solid-state diode laser and up to four fluorescence detectors for simultaneous measurement of multiple cellular parameters.

Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit 1 by BD

Sourced in United States

The Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit is a laboratory reagent used for the detection and quantification of apoptosis in cell populations. It contains Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. Annexin V is conjugated to the fluorescent dye FITC, allowing for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

Fetal bovine serum (fbs) by Thermo Fisher Scientific

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Fetal Bovine Serum (FBS) is a cell culture supplement derived from the blood of bovine fetuses. FBS provides a source of proteins, growth factors, and other components that support the growth and maintenance of various cell types in in vitro cell culture applications.

Cellquest software by BD

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CellQuest software is a data acquisition and analysis software designed for flow cytometry applications. It provides tools for acquiring, processing, and analyzing flow cytometry data.

Propidium iodide by Merck Group

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Propidium iodide is a fluorescent dye commonly used in molecular biology and flow cytometry applications. It binds to DNA and is used to stain cell nuclei, allowing for the identification and quantification of cells in various stages of the cell cycle.

What is Annexin A1 and what are its key functions?

Annexin A1 is a calcium-dependent phospholipid-binding protein that plays a crucial role in regulating inflammation and cell signaling. As a membrane-associated protein, it is involved in a variety of cellular processes, including exocytosis, endocytosis, and apoptosis. Annexin A1 has emerged as a promising therapeutic target for managing inflammatory disorders, cancer, and other diseases.

How can PubCompare.ai help with Annexin A1 research?

PubCompare.ai's AI-driven platform can enhance your Annexin A1 research in several ways. First, it allows you to screen protocol literature more efficiently, helping you identify the most reproducible and accurate experimental approaches. Second, the platform's intelligent comparisons can highlight key differences in protocol effectiveness, enabling you to choose the best option for your specific research goals and ensure optimal reproducibility and accuracy.

What are some common usage challenges with Annexin A1 research?

One of the key challenges in Annexin A1 research is identifying the most effective and reproducible experimental protocols from the vast amount of scientific literature. Researchers may also encounter difficulties in comparing and evaluating different protocol variations to determine the optimal approach for their specific research objectives. PubCompare.ai's platform can help address these challenges by leveraging AI to pinpoitnt critical insights and assist researchers in selecting the most effective Annexin A1 protocols.

Are there different variations or types of Annexin A1?

Yes, there are several variations and isoforms of Annexin A1 that have been identified. These different forms can exhibit slightly different functional properties and may be involved in specific cellular processes or disease states. Understanding the unique characteristics and applications of these Annexin A1 variants can be important for designing targeted experiments and therapies.

What are some of the key applications of Annexin A1?

Annexin A1 has a wide range of applications in various fields of research and medicine. It is a pivotal player in regulating inflammatory responses, making it a promising target for managing inflammatory disorders. Additionally, Annexin A1 has been implicated in cancer development and progression, suggesting its potential use in oncology research and therapy. Furthermore, its role in cellular processes like exocytosis and endocytosis has led to investigations into its use in membrane trafficking and signaling studies.

More about "Annexin A1"

Annexin A1, also known as lipocortin 1, is a calcium-dependent phospholipid-binding protein that plays a crucial role in regulating inflammation and cell signaling.
This membrane-associated protein is involved in a variety of cellular processes, including exocytosis, endocytosis, and apoptosis.
Annexin A1 has emerged as a promising therapeutic target for managing inflammatory disorders, cancer, and other diseases.
Researchers can leverage the power of PubCompare.ai's AI-driven platform to identify the most reproducible and accurate experimental protocols for studying Annexin A1, enabling them to elevate their research and accelerate scientific discoveries.
The platform helps researchers locate the most reliable and reproducible protocols from scientific literature, pre-prints, and patents, allowing them to make informed decisions about their experimental approaches.
The FITC Annexin V Apoptosis Detection Kit I and PE Annexin V Apoptosis Detection Kit I are commonly used tools for detecting and quantifying apoptosis, a process in which Annexin A1 plays a key role.
These kits utilize Annexin V, a protein that binds to phosphatidylserine, a marker of apoptotic cells.
When combined with a DNA-binding dye like propidium iodide, these kits can distinguish between early apoptotic, late apoptotic, and necrotic cells.
Researchers often employ flow cytometry techniques, such as the FACSCalibur, FACSCanto II, and CellQuest software, to analyze and quantify Annexin A1-related processes, including apoptosis and cell signaling.
These powerful tools allow for the precise measurement and characterization of cellular events, enabling researchers to gain valuable insights into the role of Annexin A1 in various biological systems.
By leveraging the comprehensive resources and AI-driven capabilities of PubCompare.ai, researchers can elevate their Annexin A1 research, identify the most reliable experimental protocols, and accelerate their scientific discoveries, ultimately contributing to advancements in the understanding and treatment of inflammatory disorders, cancer, and other diseases.