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Antibodies, Anti-DNA

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Most cited protocols related to «Antibodies, Anti-DNA»

1
Improved Molecular Dynamics Force Field for DNA
Cited 15 times
The AMBER simulations were carried out with the ff9931 (link), bsc08 (link), χOL39 ,10 (link) and χOL4 (this work) versions of the Cornell et al. force field.5 Bsc0 introduced significant modification to α/γ backbone torsional parameters essential for stability of B-DNA simulations. The χOL3 parametrization modified the χ glycosidic torsions to stabilize RNA simulations. While χOL3 modification can be combined with either ff99 or bsc0 for RNA, it works best in combination with bsc0. All DNA simulations must include bsc0.
ParmχOL4 is a new version of the χ-profile which aims to improve description of the syn region and syn-anti balance while not deteriorating the B-DNA simulations by the anti to high-anti region. The syn region of χOL4 profile differs from that of the RNA χOL3 force field in that it provides narrower and somewhat deeper syn valley than χOL3, thus suppressing the excessive population of χ syn angles in the region of 90–110°. This is a result of using a deoxyribonucleoside model compound instead of the ribonucleoside one for fitting, i.e., the difference is consistent with the primary QM data. When compared to the ff99 force field, χOL4 shifts the syn minimum to the higher χ values by about 10° and apparent are also differences in the barrier heights (Figure 2). Also, the syn minimum is somewhat deeper, which is an opposite trend compared to the χOL3 modification. While the syn region was fitted to the deoxyribonucleoside QM data, the anti to high-anti region has been modified empirically. The reason is that subtle increase of the slope of the χ correction between the anti and high-anti regions as compared to the ff99 supports helical twist of B-DNA. It subtly increases the helical twist of B-DNA (see below) though it remains to be seen if this change can be significant for B-DNA modeling. However, the change is probably in the right direction.
Krepl M., Zgarbová M., Stadlbauer P., Otyepka M., Banáš P., Koča J., Cheatham TE I.I.I., Jurečka P, & Šponer J. (2012). Reference simulations of noncanonical nucleic acids with different χ variants of the AMBER force field: quadruplex DNA, quadruplex RNA and Z-DNA. Journal of chemical theory and computation, 8(7), 2506-2520.
Publication 2012
11-dehydrocorticosterone Amber Antibodies, Anti-DNA Cardiac Glycosides Deoxyribonucleosides Helix (Snails) Ribonucleosides Vertebral Column Vision
2
Affinity Purification of Antibodies
Cited 13 times

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Publication 2016
2-Mercaptoethanol Antibodies Antibodies, Anti-DNA Antibody Affinity Antigens Chromatography, Affinity Cysteine Glycine Gravity Immune Sera Immunoglobulins lysyl-arginyl-alanyl-lysyl-alanyl-lysyl-threonyl-threonyl-lysyl-lysyl-arginine Oryctolagus cuniculus Peptides PRKDC protein, human Rabbits Resins, Plant Serum Staphylococcal protein A-sepharose Tromethamine Urea Xenopus laevis XRCC4 protein, human
3
Neutrophil Imaging and Characterization
Cited 11 times
Neutrophils were seeded on glass coverslips treated with 0.001% polylysine, allowed to settle, and treated with PMA (25 nM) or left unstimulated. Cells were fixed with 4% PFA, blocked (3% normal donkey serum, 3% cold water fish gelatin, 1% bovine serum albumin, and 0.05% Tween 20 in PBS) and incubated with primary antibodies antinuclear membrane (ab12365; Abcam); anti–H2A–H2B–DNA complex (Losman et al., 1992 (link)); anti-neutrophil elastase (in-house); anti–S. aureus (Biodesign), which were detected with secondary antibodies coupled to Cy2 or Cy3 (Dianova). Controls were done with isotype-matched controls. For DNA detection, DRAQ5 (shown) Bisbenzimide 33342 (Hoechst 33342), Sytox, and ToPro3 were used. Specimens were mounted in Mowiol and analyzed with a PlanApo 63×/1.32 NA objective on a confocal microscope (TCS-SP; Leica).
Fuchs T.A., Abed U., Goosmann C., Hurwitz R., Schulze I., Wahn V., Weinrauch Y., Brinkmann V, & Zychlinsky A. (2007). Novel cell death program leads to neutrophil extracellular traps. The Journal of Cell Biology, 176(2), 231-241.
Publication 2007
Antibodies Antibodies, Anti-DNA Antibodies, Antinuclear Bisbenzimidazole Trihydrochloride Cells Common Cold Equus asinus Fishes Gelatins HOE 33342 Immunoglobulin Isotypes Microscopy, Confocal Neutrophil neutrophil elastase, human Polylysine SERPINA1 protein, human Serum Serum Albumin, Bovine Tissue, Membrane Tween 20
4
Genetic Associations in Anti-HMGCR Myopathy
Cited 10 times
Between May 2002 and April 2010, 750 patients with suspected myopathy as defined by proximal muscle weakness, elevated CK levels, myopathic EMG findings, muscle edema on magnetic resonance imaging (MRI), and/or myopathic features on muscle biopsy were enrolled in a longitudinal study. Patients were defined as having DM if they had probable or definite disease by Bohan and Peter criteria (12 (link), 13 (link)) and IBM if they met Grigg’s criteria for possible disease (14 (link)). Serum was available from each subject and DNA samples were available from 260 subjects. Serum samples from 20 healthy controls without prior statin exposure were also obtained. All subjects were enrolled in protocols approved by the Johns Hopkins Institutional Review Board. Genotyping of the rs4149046 C allele was performed using the appropriate verified TaqMan Drug Metabolism Genotyping Assay (Applied Biosystems) on all 17 anti-HMGCR positive patients for whom DNA samples were available (see Table 1 for detailed clinical information).
Mammen A.L., Chung T., Christopher-Stine L., Rosen P., Rosen A, & Casciola-Rosen L.A. (2011). Autoantibodies against 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase (HMGCR) in Patients with Statin-Associated Autoimmune Myopathy. Arthritis and Rheumatism, 63(3), 713-721.
Publication 2010
Alleles Antibodies, Anti-DNA Biological Assay Biopsy Edema Ethics Committees, Research HMGCR protein, human Hydroxymethylglutaryl-CoA Reductase Inhibitors Metabolism Muscle Tissue Muscle Weakness Myopathy Patients Pharmaceutical Preparations Serum
5
Comprehensive SLE Patient Cohort Analysis
Cited 6 times
Two hundred and twenty-two patients with SLE (198 women and 24 men; mean age 51 years; range 18–88) taking part in the prospective follow-up programme KLURING (a Swedish acronym for ‘Clinical LUpus Register In Northeastern Gothia’) at the Rheumatology Clinic, Linköping University Hospital, Sweden were included between September 2008 and November 2012. This corresponds to about 95% of the expected SLE cases in the catchment area of Linköping and ≥98% of all known SLE cases. The patient material was recently described in detail.35 (link) A total of 178 patients (80%) met the ACR-82 criteria,3 (link) and 44 (20%) had a clinical diagnosis of SLE based on a history of abnormal ANA titre (specified below), and at least two typical organ manifestations at the time of diagnosis (referred to as the Fries’ criteria).36
37 (link) The presence of anticardiolipin antibodies of IgG and/or IgM class detected by ELISA and/or positive lupus anticoagulant test (not classified as an immunological criterion according to ACR-82) was found in 31 of the 44 individuals (70%) in the Fries’ group.
Patients were consecutively recruited; most were prevalent cases (85%), but some (15%) had newly diagnosed SLE at the time of enrolment. Distribution of age at disease onset is demonstrated in figure 1. The median disease duration by year 2012 was 12 years (mean 13.4; range 0–49). Disease severity/organ damage was estimated using the SLICC/ACR damage index (SDI) at the end of year 2011 or from the last observation made.38 (link) Two hundred and six (93%) of the patients were Caucasians. Ninety-two (41%) of the patients were prescribed antimalarials (AM) alone, 68 (31%) other disease-modifying antirheumatic drugs±AM and 128 (58%) oral glucocorticoids. IF-ANA staining patterns, anti-ENA reactivity and dsDNA antibodies were analysed on a routine basis at the Clinical immunology laboratory, Linköping university hospital and were extracted from medical records. In many patients, IF-ANA analysis was performed at several occasions over time but discrepant staining patterns were achieved in less than 5% of these cases. Herein, IF-ANA staining pattern from the time-point most adjacent to SLE onset was used for comparisons with clinical and laboratory features.
Frodlund M., Dahlström Ö., Kastbom A., Skogh T, & Sjöwall C. (2013). Associations between antinuclear antibody staining patterns and clinical features of systemic lupus erythematosus: analysis of a regional Swedish register. BMJ Open, 3(10), e003608.
Publication 2013
Antibodies, Anti-DNA Antibodies, Anticardiolipin Antimalarials Antirheumatic Drugs, Disease-Modifying Caucasoid Races Clinical Laboratory Services Enzyme-Linked Immunosorbent Assay Glucocorticoids Lupus Coagulation Inhibitor Lupus Vulgaris Patients Woman

Most recents protocols related to «Antibodies, Anti-DNA»

1
Antibody-based Protein Analysis in DNA Damage Response
The following commercial antibodies were used to immunoblotting: anti-phospho-H2AX (S139) (EMD Millipore, 05–636), anti-H2AX (Cell Signaling Technology, 7631), anti-DNA-PKcs phospho-S2056 (Abcam, ab124918), anti-DNA-PKcs (Abcam, ab70250), Ku80 (Santa Cruz, sc5280), Ku70 (Santa Cruz, sc17789), anti-APE1(Abcam, 189474), anti-APE1(Abcam, ab192), anti-ATM phospho-S1981 (Abcam, ab81292), anti-KAP1 phospho-S824 (Abcam, ab70369), anti-LIG4 antibody (Cell Signaling Technology, 14649), cleaved-PARP(ab32064), cleaved-Caspase3(ab32042), Artemis (Cell Signaling Technology,13381), Artemis(Invitrogen, PA5-102814), Ubb (Cell Signaling Technology, 3936), Anti-DDDDK tag (Abcam, ab1162). Secondary antibodies included anti-mouse IgG (HRP-linked) and anti-rabbit IgG (HRP-linked), which were purchased from Bio-Rad. anti-tubulin (Cell Signaling Technology, 2144), anti-actin (Abcam, ab8227), anti-Histone H3 antibody (Cell Signaling Technology, 9715). The following commercial antibodies were used to fluorescent immunostaining: γ-H2AX (EMD Millipore, 05–636) or 53BP1 (Santa Cruz, sc517218) or Cyclin A2 (Abcam, ab181591). Secondary antibodies included Fluor 488 (Cell Signaling Technology, 4412) and Alexa Fluor 562 (Cell Signaling Technology, 8889S). The following commercial antibodies were used to co-immunoprecipitation: anti-APE1 (Abcam, ab192). Immunoblotting [18 (link)], fluorescent immunostaining [19 ] analysis and co-immunoprecipitation [20 ] was performed following procedures described previously. All quantified data are derived from at least three independent experiments.
Zhang Q., Yang L., Gao H., Kuang X., Xiao H., Yang C., Cheng Y., Zhang L., Guo X., Zhong Y, & Li M. (2023). APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress. Journal of Translational Medicine, 21, 183.
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Publication 2023
AB192 compound Actins anti-IgG Antibodies Antibodies, Anti-DNA Antibodies, Anti-Idiotypic APEX1 protein, human Caspase 3 Co-Immunoprecipitation Cyclin A2 Histone H3 LIG4 protein, human lysyl-arginyl-alanyl-lysyl-alanyl-lysyl-threonyl-threonyl-lysyl-lysyl-arginine Mus PRKDC protein, human Rabbits TP53BP1 protein, human TRIM28 protein, human Tubulin Xrcc6 protein, human
2
Retrospective Study of Primary Sjögren's Syndrome
Cited 1 time
Based on computerised and paper medical files, we retrospectively collected sex, age at beginning of symptoms and age at diagnosis of pSS. We gathered Sjögren’s-related symptoms such as sicca, parotid gland enlargement and extraglandular manifestations of pSS including occurrence of lymphoma at any time before or after diagnosis. Biological and immunological features included anti-nuclear antibodies (ANA), anti-SSA/Ro, anti- SSB/La, anti-DNA, anti-Sm and anti-RNP, gammaglobulins and β2-microglobulinaemia titres, complement levels and presence of cryoglobulinaemia. We gathered histological data of accessory salivary glands biopsies. We assessed EULAR Sjögren’s Syndrome Disease Activity Index (ESSDAI) at each consult with available information and retained each category’s maximum ESSDAI score during the follow-up. We then calculated a cumulative ESSDAI (cumESSDAI) score per patient as the sum of each category’s maximum score during the follow-up. Using the same method, we calculated a cumulative ClinESSDAI (cumClinESSDAI) score, a variant which excludes the biological domain.11 (link) We additionally calculated a median EULAR Sjögren’s Syndrome Patient Reported Index score per patient when possible. Lastly, we gathered any pSS-related treatments prescribed at diagnosis and onward.
Beydon M., Seror R., Le Guern V., Chretien P., Mariette X, & Nocturne G. (2023). Impact of patient ancestry on heterogeneity of Sjögren’s disease. RMD Open, 9(1), e002955.
Publication 2023
Antibodies, Anti-DNA Antibodies, Antinuclear Biopharmaceuticals Biopsy Cryoglobulinemia Diagnosis Hypertrophy Lymphoma Parotid Gland Patients Salivary Glands Sjogren's Syndrome
3
Fecal Microbiome Profiling via 16S rDNA Sequencing
Fecal specimens were collected from patients at onset and also from HCs and stored at -80°C in a sterile preservation tube containing an anti-DNA degradation solution.
Following the manufacturer’s instructions, DNA was extracted from fecal samples using the QIAamp Fast DNA Stool Mini Kit (Qiagen, CA, USA). Detection of the isolated DNAs was determined by spectrophotometry (Multiskan™ GO, Thermo Fisher Scientific, USA). The DNA extracts also were checked by 1.5% agarose gel electrophoresis in 1× Tris-Acetate-EDTA buffer. Reaction volumes for polymerase chain reaction (PCR)in a total of 20 μL consisted of 10 μL KAPA HiFi Hot Start Ready Mix (KAPA Biosystems, MA, USA), 2 μL DNA of approximately 30 ng/μL and 1 μL forward and reverse primers of 10 μM. The 16S rDNA primer sequences were forward 5’-GTGCCAGCMGCCGCGGTAA-3’; reverse 5′-GGACTACNVGGGTWTCTAAT-3′. PCR reaction conditions were configured as required. Products were analyzed using the Qubit 3.0 (Thermo Fisher Scientific, MA, USA) to determine concentrations and then mixed in equal amounts to create a sequencing library. Insert fragments and library molar concentrations were detected and quantified using a QSEP100 (Bioptic, Taiwan) and an ABI7300 fluorescence quantitative PCR instrument (Thermo Fisher Scientific, MA, USA) was used to generate a qualified library for sequencing on the MiniSeq Illumina platform (Illumina, CA, USA). Public access to the original datasets is accessible. This data can be found at https://www.ncbi.nlm.nih.gov/sra/PRJNA906033.
The raw reads were assembled with Flash (22 (link)). Primers were removed and clean tags were generated by deleting lower reads with the use of cutadapt (23 (link)). The sequences were clustered into operational taxonomic units (OTUs) at 97% similarity using UCHIME in reference mode (24 (link)). Representative OTU sequences were aligned in the Silva_132_97_16S database (25 (link)) for taxonomic classification using the RDP Classifier (26 (link)).
Lin Z., Mao D., Jin C., Wang J., Lai Y., Zhang Y., Zhou M., Ge Q., Zhang P., Sun Y., Xu K., Wang Y., Zhu H., Lai B., Wu H., Mu Q., Ouyang G, & Sheng L. (2023). The gut microbiota correlate with the disease characteristics and immune status of patients with untreated diffuse large B-cell lymphoma. Frontiers in Immunology, 14, 1105293.
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Publication 2023
Antibodies, Anti-DNA Biologic Preservation Biopsy DNA, Ribosomal DNA Library Electrophoresis, Agar Gel Feces Fluorescence Molar Oligonucleotide Primers Patients Polymerase Chain Reaction Spectrophotometry Sterility, Reproductive tris-acetate-EDTA buffer
4
Analysis of RECQ4 and APC Complexes
Primary antibodies used were rabbit anti-RECQ4 (4-11) generated against residues 71–80 of human RECQ4 (WB 1:1000)10 (link), rabbit anti-RECQ4 (17008-1-AP, Proteintech, IP 1:200), mouse anti-RECQ4 (sc-518189, Santa Cruz, WB 1:1000), mouse anti-alpha tubulin (sc-5286, Santa Cruz, WB 1:1000), rabbit anti-APC1 (21748-1-AP, Proteintech, WB 1:1000), rabbit anti-APC5 (AP7109, Abclonal, WB 1:1000), mouse anti-APC11 (sc-517142, Santa Cruz, WB 1:1000), rabbit anti-beta actin (20536-1-AP, Proteintech, WB 1:1000), rabbit anti-BUB3 (27073-1-AP, Proteintech, WB 1:1000), mouse anti-BUB3 (sc-376506, Santa Cruz, WB 1:1000), rabbit anti-C1QBP (P32) (5734S, Cell Signaling, WB 1:1000), rabbit anti-CDC20 (10252-1-AP, Proteintech, WB 1:1000), rabbit anti-CDC45 (A2047, Abclonal, WB 1:1000), mouse anti-CDC6 (sc-9964, Santa Cruz, WB 1:1000), rabbit anti-CDT1 (A16576, Abclonal, WB 1:1000), rabbit anti-Cyclin A (PA5-16519, Thermo Fisher Scientific, WB 1:1000), mouse anti-Cyclin E (ab3927, Abcam, WB 1:1000), rabbit anti-DNA pol δ (sc-10784, Santa Cruz, WB 1:1000), rabbit anti-FBXO5 (EMI1) (10872-1-AP, Proteintech, WB 1:1000), mouse anti-FLAG (66008-3-Ig, Proteintech, WB 1:1000), rabbit anti-FLAG (20543-1-AP, Proteintech, WB 1:3000), rabbit anti-FZR1 (CDH1) (16368-1-AP, Proteintech, WB 1:1000), rabbit anti-Geminin (10802-1-AP, Proteintech, WB 1:1000), rabbit anti-GINS4 (A8592, Abclonal, WB 1:1000), rabbit anti-Histone H3 (sc-10809, Santa Cruz, WB 1:1000), rabbit anti-Lamin A/C (sc-20681, Santa Cruz, WB 1:1000), rabbit anti-MCM7 (ab52489, Abcam, WB 1:1000), rabbit anti-MCM2 (10513-1-AP, Proteintech, WB 1:1000), mouse anti-MCM5 (sc-165994, Santa Cruz, WB 1:1000), rabbit anti-ORC2 (A302-734A, Bethyl, WB 1:1000), rabbit anti-PSF2 (GINS2) (16247-1-AP, Proteintech, WB 1:1000), mouse anti-GINS2 (sc-376595, Santa Cruz, WB 1:1000), rabbit anti-PP2A (2039S, Cell Signaling, WB 1:1000), rabbit anti-SKP2 (A302-436A, Bethyl, WB 1:1000), rabbit anti-UBE1 (UBA1) (671981-1-Ig, Proteintech, WB 1:1000), rat anti-BrdU/CldU (MCA2060T, Bio-Rad, IF 1:200), mouse anti-BrdU/IdU (347580, BD Biosciences, IF 1:200), goat anti-rat IgG (H + L) Alexa Fluor Plus 488 conjugated (A48262, Invitrogen, IF 1:200), goat anti-mouse IgG (H + L) Alexa Fluor 568 conjugated (A-11004, Invitrogen, IF 1:200), goat anti-rat IgG (H + L) DyLight 488 (SA5-10018, Invitrogen, Flow Cytometry 1:100).
Xu X., Chang C.W., Li M., Omabe K., Le N., Chen Y.H., Liang F, & Liu Y. (2023). DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation. Nature Communications, 14, 1233.
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Publication 2023
alexa 568 alexa fluor 488 alpha-Tubulin anti-IgG Antibodies Antibodies, Anti-DNA beta-Actin Bromodeoxyuridine CCNE1 protein, human CDH1 protein, human Cyclin A FBXO5 protein, human Flow Cytometry FZR1 protein, human GMNN protein, human Goat Histone H3 Homo sapiens LMNA protein, human MCM2 protein, human MCM5 protein, human Mice, House ORC2L protein, human Protein Phosphatase 2A Rabbits RECQL4 protein, human SKP2 protein, human UBA1 protein, human
5
Immunoprecipitation and Immunoblotting of NLRP3 Inflammasome
Following treatment, the harvested cells were lysed in RIPA buffer with phosphatase and protease inhibitors as previously described [5 (link)]. Immunoprecipitations from specimen plasma were isolated using Protein A and G Agarose Fast Flow beads (Millipore Sigma, Burlington, MA, USA) according to the manufacturer’s protocol and using 4 µg of anti-ox-DNA antibody. The following antibodies were used: caspase-1, NF-κB p65, LC3, TLR9, Histone H3, caspase-3, PARP, IRF7, phospho-TBK1, phospho-IRF7, phospho-IRF3 (Cell Signaling Technology, Inc., Danvers, MA, USA), Human IL-1 beta/IL-1F2 (R&D Systems, Inc., Minneapolis, MN, USA), anti-oxDNA/RNA (Abcam), Anti-NLRP3 Antibody (Millipore Sigma), ASC (Santa Cruz), β-actin (Sigma-Aldrich), and appropriate Amersham ECL HRP Conjugated Antibodies (Thomas Scientific, Swedesboro, NJ, USA). Apoptosis positive control was A431 Whole Cell Lysate EGF Stimulated (Rockland Immunochemicals, Inc., Limerick, PA, USA).
To assess ASC oligomerization, cell pellets were harvested and lysed. The pellet was then resuspended in PBS and fresh 2 mM Disuccinimidyl suberate (DSS, Thermo Scientific), and incubated on a rotator for 30 min at room temperature. Following this DSS-crosslinking, the resultant was again pelleted for 10 m at 5000 rpm at 4 °C prior to blotting.
Ward G.A., Dalton RP I.I.I., Meyer B.S., McLemore A.F., Aldrich A.L., Lam N.B., Onimus A.H., Vincelette N.D., Trinh T.L., Chen X., Calescibetta A.R., Christiansen S.M., Hou H.A., Johnson J.O., Wright K.L., Padron E., Eksioglu E.A, & List A.F. (2023). Oxidized Mitochondrial DNA Engages TLR9 to Activate the NLRP3 Inflammasome in Myelodysplastic Syndromes. International Journal of Molecular Sciences, 24(4), 3896.
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Publication 2023
Actins Antibodies Antibodies, Anti-DNA Antibodies, Anti-Idiotypic Apoptosis Buffers Caspase 1 Caspase 3 Cells disuccinimidyl suberate Histone H3 Homo sapiens Immunoprecipitation Interleukin-1 beta IRF3 protein, human IRF7 protein, human Pellets, Drug Phosphoric Monoester Hydrolases Plasma Protease Inhibitors Radioimmunoprecipitation Assay Sepharose Staphylococcal Protein A TBK1 protein, human Transcription Factor RelA

Top products related to «Antibodies, Anti-DNA»

1
Cell death detection elisa kit by Roche
Sourced in United States, Germany, Switzerland, Spain, France
The Cell Death Detection ELISA kit is a quantitative in vitro assay for the detection and quantification of DNA fragmentation, a hallmark of apoptosis. The kit uses an enzyme-linked immunosorbent assay (ELISA) technique to detect and measure cytoplasmic histone-associated DNA fragments.
2
Cell death detection elisaplus kit by Roche
Sourced in Germany, United States, Switzerland, Spain, France, Italy, United Kingdom, China
The Cell Death Detection ELISAPLUS kit is a quantitative in vitro assay used for the detection and quantification of cytoplasmic histone-associated DNA fragments, which are indicative of apoptosis. The kit provides a simple, fast, and reliable method for the measurement of cell death.
3
Cell death elisaplus by Roche
Sourced in United States, Switzerland
The Cell Death ELISAPLUS is a laboratory assay used to quantify cell death. It measures the amount of cytoplasmic histone-associated DNA fragments in the cell lysate, which are indicators of apoptosis or cell death. The assay provides a sensitive and specific method for the detection and quantification of cell death.
4
Cell death detection elisaplus by Roche
Sourced in United States, Switzerland, Germany, Japan, Denmark, United Kingdom
The Cell Death Detection ELISAPLUS is an in vitro diagnostic kit that is used for the quantitative determination of cytoplasmic histone-associated DNA fragments (mono- and oligonucleosomes) in cell lysates. It is based on a sandwich-enzyme-immunoassay principle.
5
Cell death detection elisa by Roche
Sourced in United States, Germany, Switzerland
The Cell Death Detection ELISA is a quantitative in vitro assay for the detection and quantitation of DNA fragmentation, which is a hallmark of apoptosis. The assay is based on the measurement of mono- and oligonucleosomes in the cytoplasmic fraction of cell lysates.
6
Anti dna pkcs by Abcam
Sourced in United Kingdom
Anti-DNA-PKcs is a laboratory product used for the detection and analysis of DNA-PKcs, a protein involved in DNA repair processes. This product is intended for research use only and its core function is to provide a tool for researchers to study the role and regulation of DNA-PKcs in various biological systems.
7
Anti dna pkcs by Santa Cruz Biotechnology
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Anti-DNA-PKcs is a monoclonal antibody that recognizes the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a critical component of the non-homologous end joining (NHEJ) pathway, which is responsible for the repair of DNA double-strand breaks. The antibody can be used for applications such as immunoprecipitation, immunoblotting, and immunohistochemistry to study the role of DNA-PKcs in various cellular processes.
8
Quest 5 hmc dna elisa kit by Zymo Research
Sourced in United States
The Quest 5-hmC DNA ELISA Kit is a laboratory product designed for the quantitative detection of 5-hydroxymethylcytosine (5-hmC) in DNA samples. It utilizes an enzyme-linked immunosorbent assay (ELISA) format to measure the levels of 5-hmC present in the DNA.
9
Anti dna pkcs by Thermo Fisher Scientific
Sourced in United Kingdom
Anti-DNA-PKcs is a monoclonal antibody that specifically binds to the DNA-dependent protein kinase catalytic subunit (DNA-PKcs), a critical component of the non-homologous end-joining (NHEJ) DNA repair pathway. The antibody can be used to detect and quantify DNA-PKcs in various research applications.
10
Anti β actin by Merck Group
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Anti-β-actin is a laboratory reagent used to detect and quantify the presence of the β-actin protein, which is a widely expressed cytoskeletal protein found in eukaryotic cells. It is commonly used as a control or reference protein in various biochemical and cell biology techniques, such as Western blotting and immunocytochemistry.

More about "Antibodies, Anti-DNA"

Antibodies, Anti-DNA research is a critical field in biomedical science, focusing on the development and utilization of antibodies that specifically target DNA molecules.
These antibodies play a crucial role in the detection, diagnosis, and understanding of various diseases and biological processes.
One key aspect of Antibodies, Anti-DNA research is the use of ELISA (Enzyme-Linked Immunosorbent Assay) kits, such as the Cell Death Detection ELISA kit, Cell Death Detection ELISAPLUS kit, and Cell Death ELISAPLUS.
These kits allow for the quantitative measurement of DNA fragmentation, a hallmark of cell death, and are widely used in apoptosis (programmed cell death) studies.
Another important area within this field is the investigation of Anti-DNA-PKcs (DNA-dependent protein kinase catalytic subunit) antibodies.
These antibodies target a key enzyme involved in DNA repair and are utilized in the study of various DNA-related processes and disorders.
The Quest 5-hmC DNA ELISA Kit is another valuable tool in Antibodies, Anti-DNA research, enabling the detection and quantification of 5-hydroxymethylcytosine (5-hmC), an epigenetic modification of DNA that plays a crucial role in cellular function and development.
Additionally, the use of Anti-β-actin antibodies, which target a ubiquitous cytoskeletal protein, is often employed as a loading control or normalization factor in Antibodies, Anti-DNA-related experiments, ensuring the reliability and accuracy of the results.
PubCompare.ai's AI-driven optimization can enhance the reproducibility and accuracy of Antibodies, Anti-DNA research by leveraging its platform to locate the best protocols from literature, preprints, and patents, using intelligent comparisons to identify the most effective products and methods.
This cutting-edge technology can help improve research outcomes and elevate the findings in this important field of study.