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Arginine methyl ester

Arginine methyl ester: A modified form of the amino acid arginine, where the guanidino group is methylated.
This compound is used in research settings to investigate the biological roles and mechanisms of arginine methylation, a post-translational modification with important implications in cellular signaling, gene regulation, and protein function.
Arginine methyl ester serves as a tool for probing the effects of increased arginine methylation in experimental models and contributes to our understanding of this key regulatory process.

Most cited protocols related to «Arginine methyl ester»

Plasma Metabolite Extraction and Lipid Profiling

Cited 29 times

Water, isopropanol, and acetonitrile were purchased from Fisher Optima. Methanol was purchased from J.T. Baker. Ammonium formate, formic acid and methyl tert-butyl ester (MTBE) were purchased from Sigma Aldrich. Authentic standard compounds were purchased from Avanti Polar Lipids Inc., CDN Isotopes, Cayman Chemical, and Sigma Aldrich.
For hydrophilic interaction chromatography-MS/MS analysis of pharmaceutical agents present in a human plasma sample, all procedures for the metabolite extraction were kept on ice. 30 µL of human plasma was added to 1000 µL cold mix-solvent (acetonitrile/isopropanol/water, 3:3:2, v/v/v) on ice, then vortexed for 10 s, and shaken for 5 min at 4 °C using the Orbital Mixing Chilling/Heating Plate (Torrey Pines Scientific Instruments). After 2 min centrifugation at 14,000 rcf, 300 µL of the supernatant was transferred to a new 1.5 mL Eppendorf tube and evaporated to dryness in a Labconco Centrivap cold trap concentrator. The dried sample was re-suspended with 60 µL (80% acetonitrile in water) including 0.038 µg/mL choline-D₉, 0.050 µg/mL TMAO-D₉, 0.020 µg/mL betaine-D₉, 10.0 µg/mL glutamine-D₅, and 1.48 µg/mL arginine-¹⁵N₂ and centrifuged for 5 min at 16,000 rcf. The 50 µL aliquot was transferred to a glass amber vial (National Scientific) with a micro-insert (Supelco).
For lipid profiling, all samples for the metabolite extraction were kept on ice and performed as described previously³⁴. 225 µL of MeOH including 1.64 µg/mL PE (17:0/17:0), 6.55 µg/mL PG (17:0/17:0), 1.10 µg/mL PC (17:0/0:0), 0.24 µg/mL sphingosine (d17:1), 0.55 µg/mL ceramide (d18:1/17:0), 0.44 µg/mL SM (d18:1/17:0), 54.5 µg/mL palmitic acid-D3, 0.44 µg/mL PC (12:0/13:0), 22.7 µg/mL cholesterol-D7, 0.27 µg/mL TAG (17:0/17:1/17:0), 2.18 µg/mL DAG (12:0/12:0/0:0), 13.1 µg/mL DAG (18:1/2:0/0:0), 4.36 µg/mL MAG (17:0/0:0/0:0), and 0.55 µg/mL PE (17:1/0:0) were added to each dried algae on ice and vortexed for 10 seconds. Then, the MTBE including 21.8 µg/mL cholesteryl ester (22:1) was added on ice and vortexed for 10 seconds. After shaking for 6 min at 4 °C in the orbital mixer, 188 µL water was added and vortexed for 20 s. After centrifugation for 2 min at 14,000 rcf, 350 µL of the supernatant was transferred to a new 1.5 mL Eppendorf tube and evaporated to dryness in the Labconco Centrivap cold trap concentrator. The dried sample was re-suspended in 108.6 µL MeOH:toluene 90:10 (v/v) with CUDA (12-[[(cyclohexylamino)carbonyl]amino]-dodecanoic acid, 50 ng/mL). After vortexing for 20 s, each sample was sonicated for 5 min at room temperature. After centrifugation for 2 min at 16,000 rcf, 50 µL of the supernatant was transferred to a glass amber vial with micro-insert. The C. reinhardtii, C. sorokiniana, and C. variabilis samples were diluted by adding 50 µL of MeOH:toluene 90:10 (v/v). Moreover, the E. gracilis sample was diluted by adding 200 µL of MeOH:toluene 90:10 (v/v).

Tsugawa H., Cajka T., Kind T., Ma Y., Higgins B., Ikeda K., Kanazawa M., VanderGheynst J., Fiehn O, & Arita M. (2015). MS-DIAL: Data Independent MS/MS Deconvolution for Comprehensive Metabolome Analysis. Nature methods, 12(6), 523-526.

Publication 2015

Murine Models of Cardiac Hypertrophy

Cited 13 times

All experiments involving animals conformed to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication 8^th edition, update 2011) and were approved by the Institutional Animal Care and Use Committee of the University of Texas Southwestern Medical Center. The studies were in compliance with all ethical regulations. C57BL/6N mice were used for wild-type (WT) studies. Tetracycline responsive elements (TRE)-Xbp1s mice were crossed with mice harbouring tetracycline transactivator (tTA) transcription factor driven by α-myosin heavy chain promoter (αMHC-tTA) to generate mice with cardiomyocyte-specific inducible overexpression of Xbp1s (Xbp1s TG) as previously described^{10 (link)}. iNOS knockout mice (Nos2^−/−, B6.129P2-Nos2tm1Lau/J) were purchased from Jackson Laboratory (Bar Harbor, Maine) to establish an in-house colony. ZSF1-obese (ZSF1-LeprfaLeprcp/Crl, strain code 378) and Wistar-Kyoto (WKY) rats were obtained from Charles River Laboratories (Wilmington, Massachusetts). Male adult (8/12 week-old) mice were used in the experiments. Analyses in rats were carried out when the animals reached 20 weeks of age. Mice and rats were maintained on a 12-hour light/dark cycle from 6 AM to 6 PM and had unrestricted access to food (#2916, Teklad for CHOW groups and D12492, Research Diet Inc. for the HFD groups) and water. N^[w]-nitro-l-arginine methyl ester (L-NAME; 0.5 g/L, Sigma Aldrich) was supplied in the drinking water for the indicated periods of time, after adjusting the pH to 7.4. L-N6-(1-iminoethyl)lysine (L-NIL, Cayman Chemical) was administered intraperitoneally (i.p.) at a dose of 80 mg/kg body weight twice a day for three days. Transverse aortic constriction (TAC or severe TAC, sTAC) was surgically induced as previously described^{24 (link)}.

Schiattarella G.G., Altamirano F., Tong D., French K.M., Villalobos E., Kim S.Y., Luo X., Jiang N., May H.I., Wang Z.V., Hill T.M., Mammen P.P., Huang J., Lee D.I., Hahn V., Sharma K., Kass D.A., Lavandero S., Gillette T.G, & Hill J.A. (2019). Nitrosative Stress Drives Heart Failure with Preserved Ejection Fraction. Nature, 568(7752), 351-356.

Publication 2019

Adult Animals Animals, Laboratory Aortic Valve Stenosis arginine methyl ester Body Weight Caimans Diet Food Institutional Animal Care and Use Committees Lysine Males Mice, House Mice, Inbred C57BL Mice, Knockout Myocytes, Cardiac Myosin Heavy Chains NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Type II NOS2A protein, human Obesity Operative Surgical Procedures Rats, Inbred WKY Rattus norvegicus Rivers Strains Tetracycline Trans-Activators Transcription Factor

Measuring Arteriolar Vasoreactivity

Cited 10 times

Resistance arterioles of ~50 μm in diameter and ~2 mm in length were carefully dissected from the skeletal muscle tissue and cleaned of fat and connective tissue. In an organ perfusion chamber, single vessels were cannulated with glass micropipettes (outer tip diameter ∼40 μm) filled with cold bicarbonate buffer consisting of 123 mmol/L NaCl, 4.4 mmol/L KCl, 2.5 mmol/L CaCl₂, 1.2 mmol/L MgSO₄, 20 mmol/L NaHCO₃, 1.2 mmol/L KH₂PO₄, and 11 mmol/L glucose. Both ends of the vessel were secured with 10‐0 nylon Ethilon monofilament suture, and the vessels were maintained at an intraluminal pressure of 20 mmHg for 30 min. Each preparation was transferred to the stage of an inverted microscope (magnification ×200) attached to a video camera, monitor, and video‐measuring device (model VIA‐100; Boeckeler Instruments, Tucson, AZ). The external bathing medium was continuously superfused with heated buffer solution (pH = 7.4 ± 0.05, Po₂ = 140 ± 10 mmHg) aerated with a gas mixture of 21% O₂–5% CO₂–74% N₂ and maintained at 37°C. The pressure was slowly increased to 100 mmHg and maintained for 30 min. Vessels were preconstricted 30–50% with ET‐1 (100–200 pM). Vessels that did not constrict >30% were excluded from the analysis. Flow was produced by simultaneously changing the heights of the reservoirs in equal and opposite directions to generate an intraluminal pressure gradient of ∆10–∆100 cmH₂O (equivalent to ~7–70 mmHg), which covers the physiological range of arteriolar pressure in the human body (Phillips et al. 2007; Grizelj et al. 2015). In separate experiments, vasoreactivity measures were determined in response to incremental doses of acetylcholine (ACh) (10⁻⁹–10⁻⁴ mol/L). Steady‐state internal arterial diameters were measured before and during intraluminal flow or ACh in the absence or presence of the NO synthase (NOS) inhibitor N^ω‐nitro‐l‐arginine methyl ester (L‐NAME; 10⁻⁴ mol/L). Maximal diameter of every vessel was determined in the presence of papaverine (10⁻⁴ mol/L), and the diameter in response to flow at a gradient of 100 cmH₂O was measured in the presence of papaverine first in one direction, and then with the gradient reversed to verify pipette resistance matching (Phillips et al. 2007).

Mahmoud A.M., Szczurek M.R., Blackburn B.K., Mey J.T., Chen Z., Robinson A.T., Bian J., Unterman T.G., Minshall R.D., Brown M.D., Kirwan J.P., Phillips S.A, & Haus J.M. (2016). Hyperinsulinemia augments endothelin‐1 protein expression and impairs vasodilation of human skeletal muscle arterioles. Physiological Reports, 4(16), e12895.

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Publication 2016

Embryonic Aortic Haemodynamics and Stem Cell Differentiation

Cited 7 times

Haemodynamic shear stress in the embryonic aorta was estimated using previously published fluid dynamic data from the developing mouse embryo10 (link),17 (link),30 (link) and assuming circular Poiseuille flow. Ainv15 CDX4-inducible mouse embryonic stem cells (iCDX4 ES) were cultured as described22 (link) and differentiated via the hanging-drop embryoid body method. Differentiated ES cells were plated at 100,000 per cm² on 95 cm² plates, and exposed to shear stress in the presence or absence of 2 mM N(G)-nitro-L-arginine methyl ester (L-NAME) or 2 mM N(G)-nitro-D-arginine methyl ester (D-NAME). Pregnant Swiss–Webster mice were purchased from Taconic farms. Noon on the day the copulation plug was found was designated 0.5 days of gestation (E0.5). PSp/AGM regions were dissected with a conservative approach, preserving the somites. Two-dimensional primary PSp/AGM cultures were optimized in the absence or presence of growth factors (ECGS, heparin, SCF, VEGF, TPO and Flt3 ligand).

Adamo L., Naveiras O., Wenzel P.L., McKinney-Freeman S., Mack P.J., Gracia-Sancho J., Suchy-Dicey A., Yoshimoto M., Lensch M.W., Yoder M.C., García-Cardeña G, & Daley G.Q. (2009). Biomechanical forces promote embryonic haematopoiesis. Nature, 459(7250), 1131-1135.

Publication 2009

Aorta arginine methyl ester Electrocardiogram Embryo Embryoid Bodies Embryonic Stem Cells flt3 ligand Growth Factor Hemodynamics Heparin Mouse, Swiss Mouse Embryonic Stem Cells Mus Pregnancy Somites Vascular Endothelial Growth Factors

Aortic Vascular Tension Measurement

Cited 7 times

Aortic segments were mounted in 10 ml organ baths as described before [19 (link)]. Isometric force was acquired at 10 Hz and was reported in mN. To avoid any vasomotor interference due to prostanoids, 10 μM indomethacin was present in all experiments. Endothelial cells were always present but the basal NO formation was inhibited by adding a combination of 300 μM N^Ω-nitro-L-arginine methyl ester (L-NAME) and 300 μM N^Ω-nitro-L-arginine (L-NNA).

Fransen P., Van Hove C.E., Leloup A.J., Martinet W., De Meyer G.R., Lemmens K., Bult H, & Schrijvers D.M. (2015). Dissecting out the Complex Ca2+-Mediated Phenylephrine-Induced Contractions of Mouse Aortic Segments. PLoS ONE, 10(3), e0121634.

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Publication 2015

A 300 Aorta Arginine arginine methyl ester Bath Endothelial Cells Indomethacin Nitroarginine Prostaglandins

Most recents protocols related to «Arginine methyl ester»

Biochemical Agents Storage Protocol

N(G)-Nitro-L-arginine methyl ester (L-NAME) and 2′,3′-O-trinitrophenyl ATP (TNP-ATP) were obtained from Tocris (Oxford, UK), diluted in distilled water and stored at −20 °C.

Ibrahim H., Retailleau K., Hornby F., Maignel J., Beard M, & Daly D.M. (2024). A Novel Catalytically Inactive Construct of Botulinum Neurotoxin A (BoNT/A) Directly Inhibits Visceral Sensory Signalling. Toxins, 16(1), 30.

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Publication 2024

Vascular Bioactive Compound Analysis

Hydroxylamine hydrochloride, pyridine, magnesium sulphate, l-phenylephrine hydrochloride (PE), acetylcholine chloride (ACh), N^G-nitro-l-arginine methyl ester (l-NAME) were bought from Sigma-Aldrich (St Luis, MO, USA). The metabolite and oxime were dissolved in DMSO (0.1% final concentration).

Palacios J., Asunción-Alvarez D., Aravena D., Chiong M., Catalán M.A., Parra C., Cifuentes F, & Paredes A. (2024). A new oxime synthesized from Senecio nutans SCh. Bip (chachacoma) reduces calcium influx in the vascular contractile response in rat aorta. RSC Advances, 14(14), 9933-9942.

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Publication 2024

Measuring Nitric Oxide Synthase Activity

Atrial and ventricular NOS activity was examined by measuring the conversion of [¹⁴C]L-arginine (Amersham) to citrulline, in the presence of an arginase inhibitor (N^ω-hydroxy-nor-Arginine; Calbiochem), by high-performance liquid chromatography (HPLC), as described previously.^{11 (link)} Four left (LA) or right atria (RA) of the same genotype were pooled together to obtain 300 µg of protein, which was used for each measurement. Likewise, two right ventricles (RV) of the same genotype were pooled together to achieve 600 µg of RV protein. Six hundred µg of protein from one LV was used to measure NOS activity. NOS activity is reported as the N omega-nitro-L-arginine methyl ester hydrochloride (L-NAME)-inhibitable fraction of arginine-to-citrulline conversion.

Nguyen M.N., Hooper C., Stefanini M., Vrellaku B., Carnicer R., Wood M.J., Simon J.N, & Casadei B. (2024). Why is early-onset atrial fibrillation uncommon in patients with Duchenne muscular dystrophy? Insights from the mdx mouse. Cardiovascular Research, 120(5), 519-530.

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Publication 2024

Pharmacological Modulation of Nociception

Paracetamol, β-caryophyllene oxide, methiothepin, glibenclamide, [1 ,2 (link),4 (link)] oxadiazolo [4,3-a]quinoxalin-1-one (ODQ), N(G)-nitro-l-arginine methyl ester hydrochloride (l-NAME), and l-arginine were obtained from Sigma-Aldrich (St. Louis, MO, USA), and naloxone was acquired from Pisa Laboratories (Mexico City, Mexico). All drugs were suspended in carboxymethylcellulose (0.1%) and prepared minutes before being given to the animals. β-caryophyllene oxide and Paracetamol were administered orally (0.1 mL/10 g of body weight). Naloxone, methiothepin, l-NAME, ODQ, l-arginine, and glibenclamide were injected intraperitoneally (0.1 mL/10 g of body weight). Formalin (2%) was prepared by diluting aqueous formaldehyde to 37% from J.T. Baker (Matsonford Rd. Radnor Township, PA, USA).

Espinosa-Juárez J.V., Arrieta J., Briones-Aranda A., Cruz-Antonio L., López-Lorenzo Y, & Sánchez-Mendoza M.E. (2024). Synergistic Antinociceptive Effect of β-Caryophyllene Oxide in Combination with Paracetamol, and the Corresponding Gastroprotective Activity. Biomedicines, 12(5), 1037.

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Publication 2024

Vascular Reactivity Pharmacological Assay

N-ω-Nitro-L-arginine methyl ester (L-NAME), phenylephrine hydrochloride, tetraethylammonium (TEA), glibenclamide, 4-aminopyridine (4-AP), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), indomethacin, atropine, propranolol, sodium chloride (NaCl), potassium chloride (KCl), sodium bicarbonate (NaHCO₃), magnesium sulfate (MgSO₄), calcium chloride (CaCl₂), potassium dihydrogen phosphate (KH₂PO₄), and glucose were all purchased from Sigma–Aldrich, Inc. (St. Louis, MO, USA).

Mariano L.N., da Silva R.D., Niero R., Cechinel Filho V., da Silva-Santos J.E, & de Souza P. (2024). Vasodilation and Blood Pressure-Lowering Effect of 3-Demethyl-2-Geranyl-4-Prenylbellidifoline, a Xanthone Obtained from Garcinia achachairu, in Hypertensive Rats. Plants, 13(4), 528.

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Publication 2024

Top products related to «Arginine methyl ester»

L name by Merck Group

Sourced in United States, Germany, United Kingdom, Sao Tome and Principe, France, Brazil, Italy, Belgium, China, Canada, Spain

L-NAME is a synthetic compound that functions as a nitric oxide synthase inhibitor. It is commonly used in research applications to study the role of nitric oxide in biological processes.

Indomethacin by Merck Group

Sourced in United States, Germany, United Kingdom, Brazil, Italy, Sao Tome and Principe, China, India, Japan, Denmark, Australia, Macao, Spain, France, New Zealand, Poland, Singapore, Switzerland, Belgium, Canada, Argentina, Czechia, Hungary

Indomethacin is a laboratory reagent used in various research applications. It is a non-steroidal anti-inflammatory drug (NSAID) that inhibits the production of prostaglandins, which are involved in inflammation and pain. Indomethacin can be used to study the role of prostaglandins in biological processes.

Nω nitro l arginine methyl ester hydrochloride l name by Merck Group

Sourced in United States, Germany, United Kingdom

Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) is a synthetic compound used in research applications. It is a nitric oxide synthase inhibitor.

Nω nitro l arginine methyl ester l name by Merck Group

Sourced in United States, Germany, Brazil, Belgium, France

Nω-nitro-L-arginine methyl ester (L-NAME) is a laboratory reagent used as a nitric oxide synthase inhibitor. It is commonly used in research applications to study the role of nitric oxide in various biological processes.

Ng nitro l arginine methyl ester l name by Merck Group

Sourced in United States, Canada

NG-nitro-L-arginine methyl ester (L-NAME) is a laboratory reagent used in research applications. It functions as a nitric oxide synthase (NOS) inhibitor, which is useful for studying the role of nitric oxide in various biological processes.

Acetylcholine by Merck Group

Sourced in United States, Germany, United Kingdom, Italy, Sao Tome and Principe, Spain, France, China, Switzerland, Macao

Acetylcholine is a chemical compound that functions as a neurotransmitter in the body. It plays a crucial role in the transmission of signals between nerve cells and muscle cells, as well as within the central nervous system.

Ng nitro l arginine methyl ester by Merck Group

Sourced in United States, Germany

NG-nitro-L-arginine methyl ester is a laboratory chemical compound that is commonly used as a research tool in the study of nitric oxide (NO) signaling and function. It is a well-known inhibitor of nitric oxide synthase (NOS), the enzyme responsible for the production of NO from L-arginine. This compound can be used to investigate the role of NO in various biological processes.

Glibenclamide by Merck Group

Sourced in United States, Germany, United Kingdom, Spain, Brazil, Mexico, Japan, Sao Tome and Principe, India

Glibenclamide is a medication used as a lab equipment product. It is a sulfonylurea drug that helps regulate blood sugar levels. The core function of Glibenclamide is to stimulate the release of insulin from the pancreas, which can be useful in research and laboratory settings.

N nitro l arginine methyl ester l name by Merck Group

Sourced in United States

N-nitro-L-arginine methyl ester (L-NAME) is a chemical compound commonly used as a research tool in laboratory settings. It functions as a nitric oxide synthase (NOS) inhibitor, which means it can be used to block the production of nitric oxide (NO) in biological systems. The core function of L-NAME is to provide researchers with a means to investigate the role of nitric oxide in various physiological and pathological processes.

Dimethyl sulfoxide (dmso) by Merck Group

Sourced in United States, Germany, United Kingdom, China, Italy, Sao Tome and Principe, France, Macao, India, Canada, Switzerland, Japan, Australia, Spain, Poland, Belgium, Brazil, Czechia, Portugal, Austria, Denmark, Israel, Sweden, Ireland, Hungary, Mexico, Netherlands, Singapore, Indonesia, Slovakia, Cameroon, Norway, Thailand, Chile, Finland, Malaysia, Latvia, New Zealand, Hong Kong, Pakistan, Uruguay, Bangladesh

DMSO is a versatile organic solvent commonly used in laboratory settings. It has a high boiling point, low viscosity, and the ability to dissolve a wide range of polar and non-polar compounds. DMSO's core function is as a solvent, allowing for the effective dissolution and handling of various chemical substances during research and experimentation.

What are the common applications of Arginine methyl ester?

Arginine methyl ester is a valuable tool used in research settings to investigate the biological roles and mechanisms of arginine methylation. This post-translational modification has important implications in cellular signaling, gene regulation, and protein function. Researchers use arginine methyl ester to probe the effects of increased arginine methylation in experimental models, contributing to our understanding of this key regulatory process.

How can PubCompare.ai assist with optimizing the use of Arginine methyl ester?

PubCompare.ai allows researchers to screen protocol literature more efficiently and leverage AI to pinpoint critical insights. The platform can help identify the most effective protocols related to Arginine methyl ester for your specific research goals. PubCompare.ai's AI-driven analysis can highlight key differences in protocol effectiveness, enabling you to choose the best option for reproducibility and accuracy, elevating your research with innovative tools and expertise.

Are there any common usage challenges with Arginine methyl ester?

One of the key challenges with using Arginine methyl ester is ensuring optimal experimental design and protocol selection. Researchers need to carefully consider factors such as concentration, incubation time, and cell type to obtain reliable and reproducible results. PubCompare.ai can assist in this process by providing a comprehensive analysis of protocols from literature, preprints, and patents, helping researchers identify the most effective approach for their specific research needs.

Are there different variations or types of Arginine methyl ester?

Yes, there are several variations of Arginine methyl ester that researchers may encounter. These include mono-methylated, asymmetric di-methylated, and symmetric di-methylated forms, each with slightly different properties and applications. Understandiing the nuances of these variations is crucial for selecting the appropriate form for your research and interpreting your results accurately. PubCompare.ai can provide insights into the unique characteristics and uses of different Arginine methyl ester variants to support your experimental design.

More about "Arginine methyl ester"

Arginine methyl ester (AME) is a modified form of the amino acid arginine, where the guanidino group is methylated.
This compound is a valuable tool in research settings, used to investigate the biological roles and mechanisms of arginine methylation, a post-translational modification with important implications in cellular signaling, gene regulation, and protein function.
AME serves as a probe for studying the effects of increased arginine methylation in experimental models, contributing to our understanding of this key regulatory process.
Related compounds like L-NAME (Nω-Nitro-L-arginine methyl ester hydrochloride), Indomethacin, and Acetylcholine are also utilized in research on arginine metabolism and nitric oxide signaling pathways.
The methylation of arginine residues can modulate protein-protein interactions, alter subcellular localization, and affect enzymatic activity, making it a critical mechanism for cellular function.
AME provides a valuable tool for dissecting these complex regulatory mechanisms and uncovering new insights into arginine methylation and its downstream effects.
Optimizing research protocols involving AME can be greatly facilitated by innovative AI-driven platforms like PubCompare.ai, which help researchers seamlessly locate the best protocols from literature, pre-prints, and patents, while providing insightful comparisons to identify the most effective solutions.
Leveraging these cutting-edge tools can elevate the quality and efficiency of AME-related research, accelerating the pace of discovery in this important field of study.